CN112891246A - Composition capable of repairing damaged skin staying up all night and application of composition in skin care product - Google Patents
Composition capable of repairing damaged skin staying up all night and application of composition in skin care product Download PDFInfo
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- CN112891246A CN112891246A CN202110415938.XA CN202110415938A CN112891246A CN 112891246 A CN112891246 A CN 112891246A CN 202110415938 A CN202110415938 A CN 202110415938A CN 112891246 A CN112891246 A CN 112891246A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/42—Amides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/965—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of inanimate origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
The invention belongs to the technical field of skin care products, and particularly relates to a composition capable of repairing overnight damaged skin and application thereof in a skin care product, which solves various skin problems caused by overnight aging in the prior art, wherein the composition capable of repairing the overnight damaged skin is prepared by compounding carnosine, a saccharomyces cerevisiae fermentation product lysate, amber and panthenol, and the composition comprises the following raw materials in parts by weight: 3-5 parts of carnosine, 3-5 parts of schizolysis product of yeast fermentation product, 1-2 parts of amber and 5-8 parts of panthenol. The composition capable of repairing the damaged skin after being taken out of the night provided by the invention can protect mitochondria, regulate mitochondrial biological clock from the source, smoothen mitochondrial activity, activate cell energy supply, improve cell activity and improve skin function, and the skin can play a self-repairing function at night, so that the healthy circulation of the skin can be maintained even if the life and rest are disturbed, and the skin is prevented from aging.
Description
Technical Field
The invention relates to the technical field of skin care products, in particular to a composition capable of repairing skin damaged all night and application of the composition in skin care products.
Background
Mitochondria are the center for the production of ATP, which is an energy-transfer substance in cells, and chemical energy stored in ATP is used to supply skin cells to perform normal operations. The mitochondrial inner membrane contains ATP-ADP carrier protein, so that the ATP in the mitochondria and the ADP outside the mitochondria are reversely exchanged, and the ATP enters the intracellular environment outside the mitochondria to provide energy for cell extraction.
The normal operation of the biological rhythm of the skin of the human body is influenced when the human body stays up, and the cells in the skin of the human body are damaged in different degrees along with the increase of the staying up time of the human body. Since mitochondria are controlled by biological clock, the division fusion mechanism of mitochondria is slowed down along with the disturbance of biological clock, so that the number of mitochondria is reduced, and the energy substance ATP in cells is different according to the amount of mitochondria. The content of APT as the cell activity motive force is also suddenly reduced along with the prolonging of the night time, and the cells can not normally metabolize and run, so that various skin problems are caused. The research and development team discovers that the skin problem is accelerated to appear when the research and development team stays up through night muscle experiments, and the root of the research and development team lies in mitochondrial rhythm disorder of a 'cell energy factory', so that the energy supply is insufficient, and the cell decline and the skin aging are accelerated. Based on the statement, the invention provides a composition capable of repairing the skin damaged all night and application thereof in skin care products.
Disclosure of Invention
The invention aims to repair various skin problems caused by staying up all night, and provides a composition capable of repairing the skin damaged all night and application thereof in a skin care product.
The composition is prepared by compounding carnosine, a schizosaccharomyces cerevisiae fermentation product lysate, amber and panthenol, and comprises the following raw materials in parts by weight: 3-5 parts of carnosine, 3-5 parts of schizolysis product of yeast fermentation product, 1-2 parts of amber and 5-8 parts of panthenol.
Preferably, the composition capable of repairing the damaged skin after being taken out all night comprises the following raw materials in parts by weight: 3.5-4.5 parts of carnosine, 3.5-4.5 parts of schizolysis product of yeast fermentation product, 1.2-1.8 parts of amber and 6-7 parts of panthenol.
Preferably, the composition capable of repairing the damaged skin after being taken out all night comprises the following raw materials in parts by weight: 4 parts of carnosine, 4 parts of schizosaccharomyces cerevisiae fermentation product lysate, 1.5 parts of amber and 6.5 parts of panthenol.
Preferably, the composition is prepared by the following method: grinding amber into fine powder according to the weight parts, then blending the fine powder with carnosine, a schizosaccharomyces cerevisiae fermentation product lysate and panthenol, and performing ultrasonic dispersion for 10-20min at room temperature to obtain the composition.
Preferably, the composition is used for preparing a skin care product capable of repairing skin damaged all night.
The invention also provides a skin care product capable of repairing the damaged skin staying up all night, which comprises the following components in percentage by weight: 1-8% of composition capable of repairing damaged skin staying up overnight, 4-7% of glycerin, 2-6% of nicotinamide, 2-6% of isopropyl myristate, 2-5% of 1, 3-butanediol, 0.5-1% of chitosamine, 0.1-0.3% of xanthan gum, 0.1-0.3% of hyaluronic acid, 0.1-0.3% of carbomer and the balance of water.
The composition capable of repairing the damaged skin staying up all night provided by the invention has the following beneficial effects:
1. the composition capable of repairing the damaged skin staying up night is obtained by compounding carnosine, a split yeast fermentation product lysate, amber and panthenol, can protect mitochondria, regulate mitochondrial biological clock from the source, dredge mitochondrial activity, activate cell energy supply, improve cell activity and improve skin function, can achieve the self-repairing function of the skin at night, can still maintain the healthy circulation of the skin and prevent skin aging even if the life and the rest are disturbed, and has the best effects of repairing the damaged skin staying up night and resisting aging when the raw materials of the composition are 4 parts by weight, 4 parts by weight of the split yeast fermentation product lysate, 1.5 parts by weight of amber and 6.5 parts by weight of panthenol.
2. The composition capable of repairing the skin damaged in a night can be applied to preparing skin care products capable of repairing the skin damaged in a night, and the obtained skin care products are safe, mild and non-irritant and have good effects of resisting oxidation, ageing and inflammation, whitening and fading spots and the like.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples.
Example one
The composition capable of repairing the overnight damaged skin is prepared by compounding carnosine, a schizosaccharomyces cerevisiae fermentation product lysosome, amber and panthenol, and comprises the following raw materials in parts by weight: 3 parts of carnosine, 3 parts of schizosaccharomyces cerevisiae fermentation product lysate, 1 part of amber and 5 parts of panthenol; the composition is prepared by the following method: grinding amber into fine powder according to the weight parts, then blending the fine powder with carnosine, a schizosaccharomyces cerevisiae fermentation product lysate and panthenol, and performing ultrasonic dispersion for 10min at room temperature to obtain the composition.
Example two
The composition capable of repairing the overnight damaged skin is prepared by compounding carnosine, a schizosaccharomyces cerevisiae fermentation product lysosome, amber and panthenol, and comprises the following raw materials in parts by weight: 3.5 parts of carnosine, 3.5 parts of schizosaccharomyces cerevisiae fermentation product lysate, 1.2 parts of amber and 6 parts of panthenol; the composition is prepared by the following method: grinding amber into fine powder according to the weight parts, then blending the fine powder with carnosine, a schizosaccharomyces cerevisiae fermentation product lysate and panthenol, and performing ultrasonic dispersion for 12min at room temperature to obtain the composition.
EXAMPLE III
The composition capable of repairing the overnight damaged skin is prepared by compounding carnosine, a schizosaccharomyces cerevisiae fermentation product lysosome, amber and panthenol, and comprises the following raw materials in parts by weight: 4 parts of carnosine, 4 parts of schizosaccharomyces cerevisiae fermentation product lysate, 1.5 parts of amber and 6.5 parts of panthenol; the composition is prepared by the following method: grinding amber into fine powder according to the weight parts, then blending the fine powder with carnosine, a schizosaccharomyces cerevisiae fermentation product lysate and panthenol, and performing ultrasonic dispersion for 15min at room temperature to obtain the composition.
Example four
The composition capable of repairing the overnight damaged skin is prepared by compounding carnosine, a schizosaccharomyces cerevisiae fermentation product lysosome, amber and panthenol, and comprises the following raw materials in parts by weight: 4.5 parts of carnosine, 4.5 parts of schizosaccharomyces cerevisiae fermentation product lysate, 1.8 parts of amber and 7 parts of panthenol; the composition is prepared by the following method: grinding amber into fine powder according to the weight parts, then blending the fine powder with carnosine, a schizosaccharomyces cerevisiae fermentation product lysate and panthenol, and performing ultrasonic dispersion for 18min at room temperature to obtain the composition.
EXAMPLE five
The composition capable of repairing the overnight damaged skin is prepared by compounding carnosine, a schizosaccharomyces cerevisiae fermentation product lysosome, amber and panthenol, and comprises the following raw materials in parts by weight: 5 parts of carnosine, 5 parts of schizosaccharomyces cerevisiae fermentation product lysate, 2 parts of amber and 8 parts of panthenol; the composition is prepared by the following method: grinding amber into fine powder according to the weight parts, then blending the fine powder with carnosine, a schizosaccharomyces cerevisiae fermentation product lysate and panthenol, and performing ultrasonic dispersion for 20min at room temperature to obtain the composition.
In order to verify the protective effect of the composition capable of repairing the damaged skin in a late season on mitochondria, the applicant designs 3 groups of cell experiments, wherein the cell experiments are divided into a control group, a late season group and a composition group in the first embodiment of the invention, and the composition capable of repairing the damaged skin in the late season prepared in the first embodiment of the invention is contrastively verified to influence on mitochondrial ATP.
Materials and methods
1. Material
1.1 Primary reagents
| Name of reagent | Manufacturer of the product | Goods number |
| Fetal bovine serum | GIBCO | 16000-044 |
| DMEM | Hyclone | SH30243.01 |
| Trypsin-EDTA digestive juice (0.25%) | Solarbio | T1300-100 |
| Mitochondrial extraction kit | Beyotime | C3601 |
| ATP content testing box | Build up in Nanjing | A095 |
1.2 preparation of solution
PBS solution: 600ml of ddH2O dissolved with 8g NaCl, 0.2g KCl, 1.44Na2HPO4And 0.24gKH2PO4Adjusting pH of the solution to 7.4 with HCl, diluting to 1L, filtering with filter, autoclaving, and storing at room temperature.
1.3 Main instruments and consumables
2 method
2.1 cell culture
The epidermal Fibroblast (fibroplast) is prepared by placing DMEM high-glucose culture solution containing 10% fetal calf serum and 1% double antibody (streptomycin qing mixture) in 37℃，5％CO2The incubator of (2) for cultivation. Cells are observed as adherent cells under a microscope, and the trypan blue staining live cell rate reaches more than 95%.
Cells in logarithmic phase are digested by pancreatin and then are inoculated into a six-well plate at the density of 30 ten thousand cells/well, after the cells grow in an adherent manner for 24 hours, the original culture medium is removed, and the cells are continuously cultured for 12 hours by using a serum-free DMEM culture medium.
Through 3 groups of cell experiments, the state change of the skin in a non-overnight state and a overnight state of the rest rule and the state change of the composition capable of repairing the overnight damaged skin prepared in the first embodiment of the invention when the skin is overnight are simulated, wherein 10% of the composition capable of repairing the overnight damaged skin is added into cells in the first embodiment of the invention.
After the 3 groups of cells are respectively cultured for 12 hours, 16 hours, 20 hours, 24 hours and 28 hours, the cells are digested by pancreatin, the states of 10 o ' clock, 0 o ' clock, 2 o ' clock in the morning, 4 o ' clock in the morning and 6 o ' clock in the morning after the skin normally works and lives for 12 hours are simulated, so that the ATP content of the skin with regular work and rest and the ATP content of the skin in late night are observed, and the ATP content of the skin in late night of the composition which is prepared in the first embodiment of the invention and can repair the damaged skin in late night is changed, and the cells are collected for subsequent detection.
2.2 mitochondrial extraction
Adding 1-2.5ml of mitochondrion separating reagent or mitochondrion separating reagent added with PMSF into 5000-ten thousand cells before use, suspending the cells lightly, and placing in ice bath for 10-15 minutes. The cell suspension was transferred to a glass homogenizer of suitable size and homogenized for about 10-30 minutes. The cell homogenate was centrifuged at 600g at 4 ℃ for 10 minutes.
2.3ATP assay
And mixing the sample and the reagent uniformly according to the kit specification, standing at room temperature for 5 minutes, adjusting the optical path to be 0.5cm at 636nm, adjusting to zero by double distilled water, and measuring the absorbance value of each tube. After data were obtained, ATP content was calculated.
Test results
The ATP content of the skin mitochondria at 10 o ' clock, 0 o ' clock, 2 o ' clock, 4 o ' clock in the morning and 6 o ' clock in the morning of the composition group cells of the first embodiment of the invention cultured with 10% of the composition capable of repairing the damaged skin in the late morning prepared in the first embodiment of the invention is simulated by culturing for 12h, 16h, 20h, 24h and 28h at different time periods as follows:
according to experimental data, under the conditions that normal skin is not in a night and the biological rhythm is stable, the mitochondrion fusion mechanism normally operates, and the mitochondrial ATP content is normally supplied from 10 nights to 6 early morning days. The content of the compound is 308.707-349.276 mu mol/gprot from 0 to 4 am, so that the normal cell activity can be stabilized, and the cell activity is stable.
When the skin is in a late-night state, the mitochondrion fusion mechanism is disordered and the amount is reduced sharply, when the point 0 to the point 4 in the morning, the ATP content is reduced to 275.781-312.985 mu mol/gprot, and the ATP energy provided by mitochondria is insufficient, so that the cells cannot be supplied for normal activities, the cell activity is reduced, and the skin function is declined.
The composition for repairing the skin damaged in the late night state has the advantages that the mitochondrion fusion mechanism of the skin in the late night state is disordered and cannot supply ATP energy required by cells, the ATP content of mitochondria is maintained by the aid of the effect of the composition for repairing the damaged skin in the late night state, the ATP content is remarkably increased to 463.091-545.770 mu mol/gprot from 0 point to 4 points in the morning, cell activity is further excited, skin function is improved, and the skin can perform a self-repairing function at night.
The compositions capable of repairing the skin damaged all night are added according to the following formulas to prepare skin care products I-V.
A skin care product capable of repairing skin damaged overnight comprises the following components in percentage by weight: 8% of composition capable of repairing damaged skin staying up overnight, 7% of glycerin, 5% of nicotinamide, 4% of isopropyl myristate, 4% of 1, 3-butanediol, 0.5% of chitosan amine, 0.3% of xanthan gum, 0.3% of hyaluronic acid, 0.1% of carbomer and the balance of water.
And (3) safety evaluation:
the test method comprises the following steps: spot test, refer to cosmetic safety specification 2015.
The test substance: corresponding skin care products one-five to which the composition capable of repairing overnight damaged skin of examples one-five was added.
Negative control: blank.
Subject: 30 women of 20-40 years old, 6 persons in each group, according with the volunteer enrollment standard of the subject, respectively test the skin care products I-V.
The test method comprises the following steps: the selection area is not more than 50mm2About 0.020 to about 0.025g of the test substance was applied to a suitable spot tester having a depth of about 1mm by the closed patch test method. The spot tester is pasted on the front arm curve side of a subject, the subject is removed after 24 hours, the skin reaction is observed after 0.5 hour, 24 hours and 48 hours after the removal, and the result is recorded according to the skin reaction grading standard in the technical Specification for cosmetic safety 2015. The scoring criteria are shown in Table 1 and the test results are shown in Table 2.
TABLE 1 skin closed Patch test skin response grading Standard
TABLE 2 Patch test results
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. The composition capable of repairing the skin damaged in the late night is characterized by being prepared by compounding carnosine, a schizosaccharomyces cerevisiae fermentation product lysate, amber and panthenol, wherein the composition comprises the following raw materials in parts by weight: 3-5 parts of carnosine, 3-5 parts of schizolysis product of yeast fermentation product, 1-2 parts of amber and 5-8 parts of panthenol.
2. The composition capable of repairing damaged skin staying up all night according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 3.5-4.5 parts of carnosine, 3.5-4.5 parts of schizolysis product of yeast fermentation product, 1.2-1.8 parts of amber and 6-7 parts of panthenol.
3. The composition capable of repairing damaged skin staying up all night according to claim 1 or 2, which is characterized by comprising the following raw materials in parts by weight: 4 parts of carnosine, 4 parts of schizosaccharomyces cerevisiae fermentation product lysate, 1.5 parts of amber and 6.5 parts of panthenol.
4. The composition for repairing damaged skin over night according to claim 3, wherein the composition is prepared by the following method: grinding amber into fine powder according to the weight parts, then blending the fine powder with carnosine, a schizosaccharomyces cerevisiae fermentation product lysate and panthenol, and performing ultrasonic dispersion for 10-20min at room temperature to obtain the composition.
5. The composition for repairing damaged skin staying up night according to claim 4, wherein the composition is used for preparing a skin care product for repairing damaged skin staying up night.
6. A skin care product capable of repairing skin damaged overnight is characterized by comprising the following components in percentage by weight: 1-8% of composition capable of repairing damaged skin staying up overnight, 4-7% of glycerin, 2-6% of nicotinamide, 2-6% of isopropyl myristate, 2-5% of 1, 3-butanediol, 0.5-1% of chitosamine, 0.1-0.3% of xanthan gum, 0.1-0.3% of hyaluronic acid, 0.1-0.3% of carbomer and the balance of water.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110415938.XA CN112891246A (en) | 2021-04-19 | 2021-04-19 | Composition capable of repairing damaged skin staying up all night and application of composition in skin care product |
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| CN202110415938.XA CN112891246A (en) | 2021-04-19 | 2021-04-19 | Composition capable of repairing damaged skin staying up all night and application of composition in skin care product |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113384496A (en) * | 2021-07-02 | 2021-09-14 | 广州今盛美精细化工有限公司 | Synthesis process and application of schizosaccharomyces cerevisiae fermentation product lysate and carnosine composition |
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| WO2009043277A1 (en) * | 2007-09-25 | 2009-04-09 | Tianjin Sinobiotech Ltd. | Skin care composition containing hsa fusion protein, method for preparation and use thereof |
| CN104666214A (en) * | 2013-11-30 | 2015-06-03 | 烟台巨先药业有限公司 | Foundation cream containing amber extract |
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| CN110538111A (en) * | 2019-09-26 | 2019-12-06 | 湖南御家化妆品制造有限公司 | Composition and application thereof in preparation of skin product for caring skin of person staying up at night |
| CN111529417A (en) * | 2020-06-08 | 2020-08-14 | 武汉马应龙大健康有限公司 | Repair eye cream with blue light resisting effect and preparation method thereof |
| CN112641687A (en) * | 2020-12-24 | 2021-04-13 | 广州筱蔻生物科技有限公司 | Whitening composition, preparation method and application |
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2021
- 2021-04-19 CN CN202110415938.XA patent/CN112891246A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6036946A (en) * | 1997-12-24 | 2000-03-14 | Shaklee Corporation | Methods for protecting skin from damaging effects of ultraviolet light |
| WO2009043277A1 (en) * | 2007-09-25 | 2009-04-09 | Tianjin Sinobiotech Ltd. | Skin care composition containing hsa fusion protein, method for preparation and use thereof |
| CN104666214A (en) * | 2013-11-30 | 2015-06-03 | 烟台巨先药业有限公司 | Foundation cream containing amber extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113384496A (en) * | 2021-07-02 | 2021-09-14 | 广州今盛美精细化工有限公司 | Synthesis process and application of schizosaccharomyces cerevisiae fermentation product lysate and carnosine composition |
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Application publication date: 20210604 |