CN112881496B - Method for identifying lonicera japonica and honeysuckle through chemical oscillation fingerprint spectrum technology - Google Patents
Method for identifying lonicera japonica and honeysuckle through chemical oscillation fingerprint spectrum technology Download PDFInfo
- Publication number
- CN112881496B CN112881496B CN202110067229.7A CN202110067229A CN112881496B CN 112881496 B CN112881496 B CN 112881496B CN 202110067229 A CN202110067229 A CN 202110067229A CN 112881496 B CN112881496 B CN 112881496B
- Authority
- CN
- China
- Prior art keywords
- honeysuckle
- sample
- lonicera japonica
- oscillation
- fingerprint
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/4166—Systems measuring a particular property of an electrolyte
- G01N27/4168—Oxidation-reduction potential, e.g. for chlorination of water
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides a method for identifying lonicera japonica and honeysuckle by using a chemical oscillation fingerprint spectrum technology, which is characterized by comprising the following steps of: the method comprises the following steps: (1) Adding an oscillation system solution into a sample to be detected, uniformly mixing, and communicating the obtained mixed solution with an electrochemical workstation through an electrode to obtain a chemical oscillation fingerprint; (2) And (3) preprocessing the chemical oscillation fingerprint and extracting characteristic parameters, and establishing a regression relation between the characteristic parameters and the content of lonicera japonica and/or lonicera japonica in the sample to be detected. The method for identifying the lonicera japonica and the honeysuckle by using the chemical oscillation fingerprint technology has the advantages of strong characteristics, high sensitivity, large parameter range and the like, and provides a simple method for qualitative and quantitative analysis of the lonicera japonica and the honeysuckle; and secondly, the sample does not need complex pretreatment during detection, and the analysis cost is low, so that the method is convenient to popularize and apply further.
Description
Technical Field
The invention belongs to the technical field of detection of traditional Chinese medicinal materials, and particularly relates to a method for identifying lonicera confusa and lonicera japonica by using a chemical oscillation fingerprint technology.
Background
Honeysuckle and lonicera confusa are both honeysuckle plants, have similar forms and are difficult to distinguish by naked eyes, and the honeysuckle plants and the lonicera confusa are classified into a pharmacopoeia catalog from Chinese pharmacopoeia (2005 edition). The flos Lonicerae is dried flower bud or flower with initial opening of Caprifoliaceae plant L.japonica thunb, and the flos Lonicerae is dried flower bud or flower with initial opening of Caprifoliaceae Lonicera macranthoides L.macroanthoides hand-Mazz. The Chinese pharmacopoeia (2020 edition) prescribes that the content measurement standard of the honeysuckle is that chlorogenic acid is more than or equal to 1.5%, phenolic acids are more than or equal to 3.8% and luteolin is more than or equal to 0.050%; the content measurement standard of the lonicera japonica is that chlorogenic acid is more than or equal to 2.0%, and the total amount of the lonicera macranthoides saponin B and the dipsacus root saponin B is more than or equal to 5.0%. In recent years, the application of honeysuckle in the fields of traditional Chinese medicine and food industry is becoming widespread, the price is continuously high, the yield of lonicera japonica is high, the price is relatively low, and the phenomenon that lonicera japonica is mixed with honeysuckle in the market and even replaces honeysuckle with lonicera japonica is frequent. Therefore, it is necessary to search an effective, simple and economical identification and analysis method for the mixture of honeysuckle and lonicera japonica.
Currently, quality control of honeysuckle and lonicera confusa and preparations is mainly carried out by measuring single component or multiple components according to High Performance Liquid Chromatography (HPLC), gas Chromatography (GC), ultraviolet-visible spectrophotometry (UV-Vis), capillary Electrophoresis (CE), liquid-mass spectrometry (HPLC-MS) and the like. The method for determining the quality evaluation of the medicinal materials by the analysis indexes of a plurality of components is very easy to give a multiplying opportunity to illegal vendors. Therefore, there is a need to develop an analysis and detection method with higher comprehensiveness and better sensitivity aiming at qualitative and quantitative discrimination of mixed medicinal materials of lonicera japonica and honeysuckle.
The chemical oscillation fingerprint spectrum is obtained by monitoring the change of the oxidation-reduction potential value of a system along with time under a certain oscillation system. The Chinese medicine is added into the system to disturb the blank oscillation system, and the obtained spectrum is called the chemical oscillation fingerprint of the Chinese medicine. The current application practice shows that the traditional Chinese medicine chemical oscillation fingerprint technology has the characteristics of strong integrity, multiple characteristic parameters, rich information, no need of complex processing of samples, low analysis cost and the like.
Disclosure of Invention
In view of the above, the invention aims to overcome the defects in the prior art, and provides a method for identifying lonicera japonica and honeysuckle by using a chemical oscillation fingerprint technology.
In order to achieve the above purpose, the technical scheme of the invention is realized as follows:
a method for identifying flos Lonicerae and flos Lonicerae by chemical oscillation fingerprint technology comprises the following steps:
(1) Adding an oscillation system solution into a sample to be detected, uniformly mixing, and communicating the obtained mixed solution with an electrochemical workstation through an electrode to obtain a chemical oscillation fingerprint;
(2) And (3) preprocessing the chemical oscillation fingerprint and extracting characteristic parameters, and establishing a regression relation between the characteristic parameters and the content of lonicera japonica and/or lonicera japonica in the sample to be detected.
Further, the oscillating system solution in the step (1) comprises acid liquor, a catalyst, a dissipater and an oxidant; the acid liquor is a dilute sulfuric acid solution; the catalyst is at least one of copper ion solution, cerium ion solution, manganese ion solution or iron ion solution; the dissipater is at least one of acetone, malonic acid, citric acid, succinic acid or hydrogen peroxide; the oxidant is at least one of perchlorate ion solution, iodate ion solution, bromate ion solution or bromate; the specific steps of adding the oscillating system solution into the sample to be detected in the step (1) are as follows: adding acid liquor, a catalyst and a dissipation object into a sample to be detected, stirring, adding an oxidant, and uniformly mixing to obtain the catalyst; the stirring step specifically comprises the following steps: stirring at 35-42deg.C and 800-1200r/min for 8-15min.
Further, the solid-to-liquid ratio of the sample to be tested in the step (1) to the oscillating system solution is 1: (100-150); the volume ratio of the acid liquor, the catalyst, the dissipater and the oxidant is 1: (0.75-1): (0.5-0.75): (0.25-0.5); the concentration of the acid liquor is 0.72-0.80mol/L; the concentration of the catalyst is 0.0018-0.0020mol/L; the concentration of the oxidant is 0.072-0.080mol/L.
Further, the characteristic parameter in the step (2) is at least one of peak-to-valley time, peak Gu Dianwei, peak top time, peak top potential, induction time, starting potential, oscillation period, oscillation frequency, oscillation life, maximum amplitude, stopping time or stopping potential; preferably, the characteristic parameter is at least one of induction time, oscillation period, peak-to-valley time, wave stopping time, peak top time or oscillation life; more preferably, the characteristic parameter is induction time.
Further, the formula of the regression relation in the step (2) is as follows: y is Y 1 ＝AX 1 In "% +B, where Y 1 For induction time, X 1 % is the content of the lonicera japonica, the range of A is 0.01-8, and the range of B is 5-3000; the formula of the regression relation in the step (2) is as follows: y is Y 2 ＝CX 2 In%d, where Y 2 For induction time, X 2 % is the content of honeysuckle, C ranges from-0.01 to 8, and D ranges from 5 to 3000.
Further, the method of the preprocessing step in the step (2) is at least one of least square smoothing, savitzky-Golay smoothing or Gauss blurring.
Further, the sample to be detected in the step (1) is obtained after crushing and sieving; the grain diameter of the screen mesh in the sieving step is 80-120 meshes; the content of the lonicera confusa in the sample to be detected in the step (1) is 0.014-100%; the honeysuckle content of the sample to be detected in the step (1) is 0-99.986%.
Further, the electrode in the step (1) specifically comprises: a platinum wire electrode is used as an indicating electrode, and a saturated calomel electrode is used as a reference electrode; the recording time range of the chemical oscillation fingerprint in the step (1) is 0-3500 seconds, and the sampling interval is 0.1-0.2 seconds.
The application of the method is that the method is used for identifying the lonicera confusa and/or the lonicera in the mixture sample.
The application of the method in determining the content of the lonicera japonica and/or the honeysuckle in the mixture sample.
Compared with the prior art, the invention has the following advantages:
the method for identifying the lonicera japonica and the honeysuckle by using the chemical oscillation fingerprint technology has the advantages of strong characteristics, high sensitivity, large parameter range and the like, and provides a simple method for qualitative and quantitative analysis of the lonicera japonica and the honeysuckle; secondly, the sample does not need complex pretreatment during detection, and the analysis cost is low, so that the method is convenient to popularize and apply further; more importantly, the quality control analysis of the medicinal materials is based on the analysis of the whole redox activity of the medicinal materials, has strong integrity, avoids the limitation problem that the detection of single or a plurality of components cannot fully reflect the quality of the medicinal materials, and provides a new method and a new thought for the whole quality evaluation of the medicinal materials.
Drawings
Fig. 1 is a chemical oscillation fingerprint of honeysuckle, a mixture of honeysuckle and lonicera japonica, and lonicera japonica according to embodiment 1 of the present invention: curve a is honeysuckle, curve B is a mixture of honeysuckle and honeysuckle, and curve C is honeysuckle;
FIG. 2 is a graph showing the linear relationship between induction time, oscillation period, peak-to-valley time, wave-stopping time, peak-top time, oscillation life and flos Lonicerae content in example 2 of the present invention: 2-A is induction time, 2-B is oscillation period, 2-C is wave stopping time, 2-D is peak-valley time, 2-E is peak top time, and 2-F is oscillation life;
FIG. 3 is a graph showing the linear relationship between the induction time and the honeysuckle flower content in example 2 of the present invention.
Detailed Description
Unless defined otherwise, technical terms used in the following examples have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention pertains. The test reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents; the experimental methods are conventional methods unless otherwise specified.
The platinum wire electrode and the saturated calomel electrode in the embodiment of the invention are purchased from Shanghai Chenhua instruments.
The honeysuckle medicinal material in the embodiment of the invention is purchased from Henan and the mountain honeysuckle medicinal material is purchased from Hunan.
The present invention will be described in detail with reference to examples.
The mixed medicinal materials in the embodiments 1-4 are prepared by crushing and sieving honeysuckle medicinal materials and wild honeysuckle medicinal materials and mixing according to a certain proportion.
Wherein, the mass fractions of the mixed medicinal material A prepared in the embodiment 1 are respectively as follows: 100% of lonicera japonica and 0 of honeysuckle; the mass fractions of the preparation mixed medicinal material B are respectively as follows: 40% of honeysuckle and 60% of honeysuckle; the mass fractions of the preparation mixed medicinal material C are respectively as follows: flos Lonicerae 0, flos Lonicerae 100%;
the mass fractions of the mixed medicinal materials D prepared in example 2 are respectively as follows: 100% of lonicera japonica and 0 of honeysuckle; the mass fractions of the prepared mixed medicinal material E are respectively as follows: 80% of lonicera japonica and 20% of honeysuckle; the mass fractions of the prepared mixed medicinal material F are respectively as follows: 60% of lonicera japonica and 40% of honeysuckle; the mass fractions of the prepared mixed medicinal material G are respectively as follows: 40% of honeysuckle and 60% of honeysuckle; the mass fractions of the prepared mixed medicinal material H are respectively as follows: 20% of lonicera japonica and 80% of honeysuckle; the mass fractions of the preparation mixed medicinal material I are respectively as follows: 0.014% of honeysuckle and 99.986% of honeysuckle;
the mass fractions of the mixed medicinal materials J prepared in example 3 are respectively as follows: 30% of lonicera japonica and 70% of honeysuckle;
the mass fractions of the prepared mixed medicinal material K in example 4 are respectively as follows: 90% of lonicera japonica and 10% of honeysuckle.
The pretreatment process of the mixed medicinal material L in the embodiment 5 of the invention is as follows: 30.7mL of commercially available wild honeysuckle flower dew (Hubei edible is Tian pharmaceutical industry Co., ltd., mark amount is 0.0625 g/mL) is taken, reduced pressure distillation is carried out, concentration is carried out to 3.07mL, 0.0307mL of concentrated liquid is taken, and 0.38g of honeysuckle flower powder is added to obtain a mixed medicinal material, wherein the mass fraction of the honeysuckle flower is 95%, the mass fraction of the honeysuckle flower is 4.8%, and the content of other impurities is 0.2%.
Example 1 chemical oscillation fingerprint test of Mixed medicinal materials containing lonicera japonica and/or lonicera japonica
0.4g of mixed medicinal material A, 0.72mol/L of dilute sulfuric acid solution, 0.0018mol/L of copper sulfate catalyst solution and acetone dissipation substance solution which are crushed and sieved by a 80-mesh sieve are sequentially added into a three-port bottle under the condition that the temperature is 35 ℃ and the rotating speed is 800r/min, the solution is stirred for 8min, 0.072mol/L of potassium bromate oxidant solution is added, and the volumes of acid liquid, catalyst, dissipation substance and oxidant are 16mL,12mL,8mL and 4mL respectively. The platinum wire electrode is used as a working electrode, the saturated calomel electrode is used as a reference electrode, an electrochemical workstation is immediately started, a chemical oscillation fingerprint is recorded until 2800 seconds, and the sampling interval is 0.1 seconds. The curve A in figure 1 is the chemical oscillation fingerprint of flos Lonicerae.
The above operation is repeated except that: and replacing the sample to be tested with the mixed medicinal material B, recording the chemical oscillation fingerprint, and adjusting to 3000 seconds under the condition that other conditions are unchanged, so as to obtain the chemical oscillation fingerprint of the mixed medicinal material containing the lonicera japonica and the honeysuckle, wherein the chemical oscillation fingerprint is shown as a curve B in fig. 1.
The above operation is repeated except that: and replacing the sample to be detected with the mixed medicinal material C, recording the chemical oscillation fingerprint, adjusting to 3500 seconds, and obtaining the chemical oscillation fingerprint of the honeysuckle medicinal material under the condition that other conditions are unchanged, wherein the chemical oscillation fingerprint is shown as a curve C in fig. 1.
Example 2 content determination method of Mixed medicinal materials containing lonicera japonica and/or lonicera japonica
0.4g of mixed medicinal material D, 0.72mol/L of dilute sulfuric acid solution, 0.0018mol/L of copper sulfate catalyst solution and acetone dissipation substance solution which are crushed and sieved by a 80-mesh sieve are sequentially added into a three-port bottle under the condition that the temperature is 35 ℃ and the rotating speed is 800r/min, the solution is stirred for 8min, 0.072mol/L of potassium bromate oxidant solution is added, and the volumes of acid liquid, catalyst, dissipation substance and oxidant are 16mL,12mL,8mL and 4mL respectively. The platinum wire electrode is used as a working electrode, the saturated calomel electrode is used as a reference electrode, an electrochemical workstation is immediately started, a chemical oscillation fingerprint is recorded to 3000 seconds, and the sampling interval is 0.1 seconds.
The above operations are repeated in sequence, except that: respectively replacing the sample to be detected with a mixed medicinal material E; chemical oscillation fingerprint of the mixed medicinal material E;
the above operations are repeated in sequence, except that: respectively replacing the sample to be detected with the mixed medicinal material F; chemical oscillation fingerprint of mixed medicinal material F;
the above operations are repeated in sequence, except that: respectively replacing the sample to be detected with the mixed medicinal materials G; chemical oscillation fingerprint of the mixed medicinal material G;
the above operations are repeated in sequence, except that: respectively replacing the sample to be detected with a mixed medicinal material H; chemical oscillation fingerprint of mixed medicinal material H;
the above operations are repeated in sequence, except that: respectively replacing the sample to be tested with the mixed medicinal materials I; chemical oscillation fingerprint of the mixed medicinal material I.
And carrying out least square smoothing treatment on all obtained chemical oscillation fingerprints, extracting characteristic parameters of peak-valley time, peak Gu Dianwei, peak top time, peak top potential, induction time, wave starting potential, oscillation period, oscillation times, oscillation service life, maximum amplitude, wave stopping time and wave stopping potential, examining the linear relation between the characteristic parameters and the content of the lonicera japonica or the lonicera japonica, and establishing an optimal test method of the content of the lonicera japonica or the lonicera japonica. FIG. 2 shows the induction time, oscillation period, peak-to-valley time, wave-stopping time,The peak top time and oscillation life are linearly related to the amount of lonicera confusa, wherein the linear correlation of the induction time and the amount of lonicera confusa is optimal, the induction time (Y 1 ) Is combined with the flos Lonicerae content (X) 1 ) According to the linear equation Y 1 ＝2.7X 1 Form% 1371.6. FIG. 3 is a linear relationship between induction time and honeysuckle content, induction time (Y 2 ) With honeysuckle flower content (X) 2 ) According to the linear equation Y 2 ＝-2.7X 2 Form% 1642.5.
Example 3 determination of the content of silver flowers in Mixed medicinal materials containing silver flowers
0.4g of the mixed medicinal material J, 0.80mol/L of dilute sulfuric acid solution, 0.0020mol/L of manganese sulfate catalyst solution and malonic acid dissipation substance solution which are crushed and sieved by a 120-mesh sieve are sequentially added into a three-port bottle under the condition that the temperature is 42 ℃ and the rotating speed is 1200r/min, the mixture is stirred for 15min, then 0.080mol/L of potassium iodate oxidant solution is added, and the volumes of acid liquid, catalyst, dissipation substance and oxidant are 16mL,12mL,8mL and 4mL respectively. The platinum wire electrode is used as a working electrode, the saturated calomel electrode is used as a reference electrode, an electrochemical workstation is immediately started, a chemical oscillation fingerprint is recorded to 3000 seconds, and the sampling interval is 0.1 seconds. The extraction induction time was 1461s, and according to the induction time (Y 1 ) Is combined with the flos Lonicerae content (X) 1 ) According to the linear equation Y 1 ＝2.7X 1 The calculated percentage of the honeysuckle flower is 33.1 percent which is basically consistent with the true value of 30 percent, which shows that the method has high accuracy.
Example 4 determination of honeysuckle content in Mixed medicine containing honeysuckle
Under the conditions of the temperature of 40 ℃ and the rotating speed of 1000r/min, 0.4g of the crushed and 100-mesh mixed medicinal material K, 0.80mol/L of dilute sulfuric acid solution, 0.0020mol/L of manganese sulfate catalyst solution and acetone dissipator solution are sequentially added into a three-mouth bottle, and after stirring for 10min, 0.080mol/L of potassium bromate oxidant solution is added, wherein the volumes of acid liquid, catalyst, dissipator and oxidant are 16mL,12mL,8mL and 4mL respectively. The platinum wire electrode is used as a working electrode, the saturated calomel electrode is used as a reference electrode, an electrochemical working station is immediately started, a chemical oscillation fingerprint is recorded to 3000 seconds, and the sampling interval is 0.1 second. The extraction induction time was 1609s, and the time (Y 2 ) With honeysuckle flower content (X) 2 ) According to the linear equation Y 2 ＝-2.7X 2 The honeysuckle content obtained by calculation of the percent+ 1642.5 is 12.4 percent and basically coincides with the true value of 10 percent, which proves that the method has high accuracy.
Example 5 content determination of Mixed medicinal materials containing lonicera japonica and honeysuckle
Adding mixed medicinal material L into a three-mouth bottle at the temperature of 40 ℃ and the rotating speed of 1000r/min, adding 0.80mol/L dilute sulfuric acid solution, 0.0020mol/L manganese sulfate catalyst solution and acetone dissipator solution, stirring for 10min, and adding 0.080mol/L potassium bromate oxidant solution, wherein the volumes of acid solution, catalyst, dissipator and oxidant are 16mL,12mL,8mL and 4mL respectively. The platinum wire electrode is used as a working electrode, the saturated calomel electrode is used as a reference electrode, an electrochemical workstation is immediately started, a chemical oscillation fingerprint is recorded to 3000 seconds, and the sampling interval is 0.1 seconds. According to the induction time 1631s, the content of the lonicera japonica is calculated to be 96%, and the content of the lonicera japonica is calculated to be 4.2% and basically coincides with a true value, so that the accuracy of the method is high.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (7)
1. A method for identifying lonicera japonica and honeysuckle by using a chemical oscillation fingerprint spectrum technology is characterized by comprising the following steps of: the method comprises the following steps:
(1) Adding an oscillation system solution into a sample to be detected, uniformly mixing, and communicating the obtained mixed solution with an electrochemical workstation through an electrode to obtain a chemical oscillation fingerprint;
(2) Preprocessing the chemical oscillation fingerprint and extracting characteristic parameters, and establishing a regression relation between the characteristic parameters and the content of lonicera japonica and honeysuckle in a sample to be detected;
the characteristic parameters in the step (2) are at least one of induction time, oscillation period, peak-valley time or wave stopping time;
the formula of the regression relation in the step (2) is as follows: y is Y 1 =AX 1 In "% +B, where Y 1 For induction time, X 1 % is the content of the lonicera japonica, the range of A is 0.01-8, and the range of B is 5-3000; the formula of the regression relation in the step (2) is as follows: y is Y 2 =CX 2 In%d, where Y 2 For induction time, X 2 % is the content of honeysuckle, the range of C is-0.01-8, and the range of D is 5-3000;
the oscillating system solution in the step (1) comprises acid liquor, a catalyst, a dissipater and an oxidant; the acid liquor is a dilute sulfuric acid solution; the catalyst is at least one of copper ion solution, cerium ion solution, manganese ion solution or iron ion solution; the dissipater is at least one of acetone, malonic acid, citric acid, succinic acid or hydrogen peroxide; the oxidant is at least one of perchlorate ion solution, iodate ion solution, bromate ion solution or bromate; the specific steps of adding the oscillating system solution into the sample to be detected in the step (1) are as follows: adding acid liquor, a catalyst and a dissipation object into a sample to be detected, stirring, adding an oxidant, and uniformly mixing to obtain the catalyst; the stirring step specifically comprises the following steps: stirring at 35-42deg.C and 800-1200r/min for 8-15min.
2. The method according to claim 1, characterized in that: the solid-liquid ratio of the sample to be tested in the step (1) to the oscillating system solution is 1: (100-150); the volume ratio of the acid liquor, the catalyst, the dissipater and the oxidant is 1: (0.75-1): (0.5-0.75): (0.25-0.5); the concentration of the acid liquor is 0.72-0.80mol/L; the concentration of the catalyst is 0.0018-0.0020mol/L; the concentration of the oxidant is 0.072-0.080mol/L.
3. The method according to claim 1, characterized in that: the method of the preprocessing step in the step (2) is at least one of least square smoothing, savitzky-Golay smoothing or Gauss blurring processing.
4. The method according to claim 1, characterized in that: the sample to be detected in the step (1) is obtained after crushing and sieving; the grain diameter of the screen mesh in the sieving step is 80-120 meshes; the content of the lonicera confusa in the sample to be detected in the step (1) is 0.014-100%; the honeysuckle content of the sample to be detected in the step (1) is 0-99.986%.
5. The method according to claim 1, characterized in that: the electrode in the step (1) is specifically: a platinum wire electrode is used as an indicating electrode, and a saturated calomel electrode is used as a reference electrode; the recording time range of the chemical oscillation fingerprint in the step (1) is 0-3500 seconds, and the sampling interval is 0.1-0.2 seconds.
6. Use of the method according to any one of claims 1-5, characterized in that: the method is applied to the identification of the lonicera japonica and the honeysuckle in the mixture sample.
7. Use of the method according to any one of claims 1-5, characterized in that: the method is applied to the determination of the content of the lonicera japonica and the honeysuckle in the mixture sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110067229.7A CN112881496B (en) | 2021-01-19 | 2021-01-19 | Method for identifying lonicera japonica and honeysuckle through chemical oscillation fingerprint spectrum technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110067229.7A CN112881496B (en) | 2021-01-19 | 2021-01-19 | Method for identifying lonicera japonica and honeysuckle through chemical oscillation fingerprint spectrum technology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112881496A CN112881496A (en) | 2021-06-01 |
| CN112881496B true CN112881496B (en) | 2023-06-23 |
Family
ID=76049616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110067229.7A Active CN112881496B (en) | 2021-01-19 | 2021-01-19 | Method for identifying lonicera japonica and honeysuckle through chemical oscillation fingerprint spectrum technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112881496B (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL1931998T3 (en) * | 2005-09-15 | 2010-11-30 | Alk Abello As | A method for quantification of allergens |
| CN107144661B (en) * | 2017-05-11 | 2018-12-07 | 湖南省中医药研究院 | Detection method that is a kind of while obtaining material composition and total antioxidant activity in Honeysuckle flower |
| CN107607598B (en) * | 2017-11-09 | 2020-09-01 | 湖南省食品质量监督检验研究院 | Method for identifying authenticity of lycium ruthenicum based on nonlinear chemical fingerprint technology |
-
2021
- 2021-01-19 CN CN202110067229.7A patent/CN112881496B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN112881496A (en) | 2021-06-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106053628B (en) | A kind of method that fast qualitative quantifies fragrance component in tealeaves | |
| CN106706794B (en) | A kind of method of main tongue fur faint scent characteristic chicken flavor substance in quantitative analysis ramuscule cigarette mainstream flue gas | |
| Wang et al. | Age-dependent characterization of volatile organic compounds and age discrimination in Chinese rice wine using an untargeted GC/MS-based metabolomic approach | |
| CN103412034A (en) | Measuring method for quickly measuring contents of heavy metals in tobacco by using microwave digestion/ICP-MS method | |
| Husáková et al. | Analytical capabilities of inductively coupled plasma orthogonal acceleration time-of-flight mass spectrometry (ICP-oa-TOF-MS) for multi-element analysis of food and beverages | |
| CN107102077B (en) | A kind of analysis method identifying acacia honey and the adulterated acacia honey of syrup | |
| CN111650297B (en) | Method for efficiently and rapidly determining content of organic acid in berry juice | |
| CN110850000A (en) | Method for quantifying honey MRJP2 by liquid chromatography-tandem mass spectrometry | |
| CN112881496B (en) | Method for identifying lonicera japonica and honeysuckle through chemical oscillation fingerprint spectrum technology | |
| CN113125409B (en) | Method for rapidly detecting butralin in tobacco by surface enhanced Raman scattering | |
| CN102539463A (en) | X-ray fluorescent spectrometry for analyzing contents of 17 elements in organic silicon contact mass | |
| Xu et al. | Determining the geographical origin of milk by multivariate analysis based on stable isotope ratios, elements and fatty acids | |
| CN106908527B (en) | A method of identifying the honey of lychee flowers place of production | |
| CN113390980A (en) | Method for evaluating flavor substance change in pancake processing | |
| Yang et al. | Advances in the analysis methods of inorganic elements in foods | |
| Nguyen et al. | Quantitative determination of bromine and iodine in food samples using ICP-MS | |
| CN102520106A (en) | Brewed soy discrimination method based on fragrance component | |
| Wang et al. | Metabolomic fingerprinting based on network analysis of volatile aroma compounds during the forced aging of Huangjiu: Effects of dissolved oxygen and temperature | |
| CN113759047B (en) | Method for detecting content of saponins in ginseng and method for identifying garden ginseng and wild ginseng | |
| CN114235988B (en) | High performance liquid chromatography for determining content of anti-sprouting pellet | |
| CN105136773A (en) | Method of rapidly detecting cyflumetofen by utilizing Raman spectra | |
| CN113984952B (en) | Method for analyzing components of fermented soy sauce | |
| CN102608050B (en) | Identification and quantitative analysis method for refined gutter oil | |
| Tan et al. | Determination of Trace Metals in Garlic Bulbs (Allium sativum L.): A Variety Discrimination by Inductively Coupled Plasma Mass Spectrometry | |
| CN111579664A (en) | Method for identifying processing quality of platycladus orientalis charcoal based on odor identification and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |