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CN112752757B - Tyrosine amide derivatives as RHO-kinase inhibitors - Google Patents

Tyrosine amide derivatives as RHO-kinase inhibitors Download PDF

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CN112752757B
CN112752757B CN201980047406.6A CN201980047406A CN112752757B CN 112752757 B CN112752757 B CN 112752757B CN 201980047406 A CN201980047406 A CN 201980047406A CN 112752757 B CN112752757 B CN 112752757B
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methyl
amino
oxy
pyrrolo
phenyl
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CN112752757A (en
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A·阿塞塔
F·兰卡蒂
A·M·卡佩利
D·E·克拉克
P·蒂塞利
C·爱德华兹
A·J·F·A·彻圭劳麦
G·巴莱
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Chiesi Farmaceutici SpA
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Abstract

The present invention relates to compounds of formula I (which are bicyclic dihydropyrimidine-carboxamide derivatives) that inhibit Rho kinase, methods for preparing such compounds, pharmaceutical compositions containing them and their therapeutic use. In particular, the compounds of the invention are useful in the treatment of a number of disorders associated with the ROCK enzyme mechanism, such as pulmonary diseases, including asthma, chronic Obstructive Pulmonary Disease (COPD), idiopathic Pulmonary Fibrosis (IPF) and Pulmonary Arterial Hypertension (PAH).

Description

Tyrosine amide derivatives as RHO-kinase inhibitors
Technical Field
The present invention relates to a compound that inhibits Rho kinase (hereinafter referred to as ROCK inhibitor); in particular, the present invention relates to compounds that are tyrosine amide derivatives, methods of preparing such compounds, pharmaceutical compositions containing them and their therapeutic uses.
The compounds of the invention are inhibitors of the activity or function of Rho-related coiled coil forming protein kinase (ROCK) isoforms of ROCK-I and/or ROCK-II.
Background
Rho-related coiled coil forming protein kinases (ROCK) belong to the AGC (PKA/PKG/PKC) family of serine-threonine kinases. Two human isoforms of ROCK have been described, ROCK-I (also known as p160 ROCK or ROKβ) and ROCK-II (ROKα) are approximately 160kDa proteins containing an N-terminal Ser/Thr kinase domain followed by a coiled-coil structure, a pleckstrin homology domain and a cysteine-rich region at the C-terminus (Riento, K.; ridley, A.J. Rocks: multifunctional kinases in cell behaviour. Nat. Rev. Mol. Cell biol.2003,4, 446-456).
ROCK-II and ROCK-I are both expressed in many human and rodent tissues, including heart, pancreas, lung, liver, skeletal muscle, kidney and brain (Riento and Ridley,2003 above). In patients with pulmonary hypertension (pulmonary hypertension), ROCK activity in lung tissue and circulating neutrophils was significantly higher than in controls (Duong-query S, bei Y, liu Z, dinh-Xuan at. Rotor of Rho-kinase and its inhibitors in pulmonary hypertension. Pharmacol ter. 2013;137 (3): 352-64). A significant correlation was established between neutrophil ROCK activity and severity and duration of pulmonary hypertension (dunng-Quy et al, 2013).
There is now a great deal of evidence that ROCK is involved in many pathways that promote pathology associated with several acute and chronic lung diseases, including asthma, COPD, bronchiectasis and ARDS/ALI. In view of the biological effects of ROCK, selective inhibitors have the potential to treat many pathological mechanisms of respiratory diseases, such as smooth muscle hyperreactivity, bronchoconstriction, airway inflammation and airway remodeling, neuromodulation, and exacerbation by respiratory viral infection (Fernandes LB, henry PJ, goldie rg. Rho kinase as a therapeutic target in the treatment of asthma and chronic obstructive pulmonary disease. Ter Adv respiratory dis.2007oct;1 (1): 25-33). In fact, rho kinase inhibitor Y-27632 causes bronchodilation and reduces pulmonary eosinophilia transport and airway hyperreactivity (Gosens, R.; schaafsma, D.; nelemans, S.A.; halayko, A.J. Rhokinase as a drug target for the treatment of airway hyperresponsiveness in aschma Mini-Rev. Med. Chem.2006,6,339-348). ROCK activation of the lung has been demonstrated in humans with Idiopathic Pulmonary Fibrosis (IPF) and in animal models of this disease. ROCK inhibitors can prevent fibrosis in these models and, more importantly, induce regression of established fibrosis, indicating that ROCK inhibitors are potentially potent pharmacological agents that prevent progression of pulmonary fibrosis (Jiang, c.; huang, h.; liu, j.; wang, y.; lu, z.; xu, z.
Various compounds have been described in the literature as Rho kinase inhibitors. See, for example, WO2004/039796, WO2006/009889, WO2010/032875, WO2009/079008, WO2014/118133 and WO2018/115383 of the same applicant.
There is still the potential to develop new and pharmacologically improved ROCK inhibitors in many therapeutic areas such as cardiovascular and respiratory diseases, erectile dysfunction, fibrotic diseases, insulin resistance, renal failure, central nervous system disorders, autoimmune diseases and oncology.
Given the number of pathological responses mediated by ROCK enzymes, there is a continuing need for inhibitors of such enzymes that can be used to treat a number of disorders. The present invention relates to novel compounds which are inhibitors of Rho-related coiled coil forming protein kinase (ROCK) isoforms of ROCK-I and ROCK-II, which have therapeutically desirable characteristics, particularly for some pulmonary diseases including asthma, chronic Obstructive Pulmonary Disease (COPD), idiopathic Pulmonary Fibrosis (IPF) and Pulmonary Hypertension (PH) and especially Pulmonary Arterial Hypertension (PAH). Our co-pending application number PCT/EP2018/052009 and the present invention address the above-mentioned need by providing compounds of this type. The compounds of the invention are active as inhibitors of ROCK-I and ROCK-II isoforms, which are potent and preferably advantageously exhibit other improved properties such as solubility.
Disclosure of Invention
The present invention relates to compounds of formula (I) which act as ROCK inhibitors, to processes for their preparation, to pharmaceutical compositions comprising them alone or in combination with one or more active ingredients in admixture with one or more pharmaceutically acceptable carriers
Wherein X is 1 、X 2 、R、R 0 、R 1 、R 2 、R 3 、R 4 、R 5 、R 6 And p is reported in the detailed description below.
In one aspect, the invention relates to a compound of formula (I) for use as a medicament. In one aspect, the invention provides the use of a compound of the invention for the preparation of a medicament.
In another aspect, the invention provides the use of a compound of the invention for the manufacture of a medicament for the treatment of any disease characterized by abnormal activity of a ROCK enzyme and/or in which inhibition of activity (and in particular selective inhibition of other kinases by a ROCK enzyme isoform) is desired.
Furthermore, the present invention provides a method for preventing and/or treating any disease in which inhibition of ROCK enzyme is desired, comprising administering to a patient in need of such treatment a therapeutically effective amount of a compound of the present invention.
In particular, the compounds of the invention, alone or in combination with other active ingredients, may be administered for the prevention and/or treatment of pulmonary diseases, including asthma, chronic Obstructive Pulmonary Disease (COPD), idiopathic Pulmonary Fibrosis (IPF) and Pulmonary Hypertension (PH) and especially Pulmonary Arterial Hypertension (PAH).
Detailed Description
Definition of the definition
The term "pharmaceutically acceptable salts" refers to derivatives of the compounds of formula (I), wherein the parent compound is suitably modified as follows: any free acidic or basic groups (if present) are converted to the corresponding addition salts with any base or acid conventionally considered pharmaceutically acceptable.
Suitable examples of such salts may thus include inorganic or organic acid addition salts of basic residues such as amino groups and inorganic or organic base addition salts of acidic residues such as carboxyl groups.
Cations of inorganic bases that may suitably be used to prepare the salts of the present invention comprise ions of alkali or alkaline earth metals such as potassium, sodium, calcium or magnesium. Those obtained by reacting a main compound functioning as a base with an inorganic acid or an organic acid to form a salt include, for example, salts of hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, methanesulfonic acid, camphorsulfonic acid, acetic acid, oxalic acid, maleic acid, fumaric acid, succinic acid, and citric acid.
Many organic compounds may form complexes with solvents in which they react or from which they precipitate or crystallize. These complexes are referred to as "solvates" and are another object of the present invention. Polymorphs and crystalline forms of a compound of formula (I) or a pharmaceutically acceptable salt thereof, or solvates thereof, are another object of the present invention.
The term "halogen" or "halogen atom" includes fluorine, chlorine, bromine and iodine atoms, preferably chlorine or fluorine, meaning fluorine, chlorine, bromine, iodine as substituents.
The term "(C) 1 -C 6 ) Alkyl "means a straight or branched chain alkyl group in which the number of carbon atoms of the component is in the range of 1 to 6. Specific alkyl groups are methyl, ethyl, n-propyl, isopropyl and tert-butyl.
Expression "(C) 1 -C 6 ) Haloalkyl "means" as defined above "(C 1 -C 6 ) An alkyl "group in which one or more hydrogen atoms are replaced by one or more halogen atoms, which may be the same or different from each other. Examples include halogenatedAlkyl groups, polyhalogenated alkyl groups and perhalogenated alkyl groups in which all hydrogen atoms are replaced by halogen atoms, such as trifluoromethyl or difluoromethyl.
By analogy, the term "(C) 1 -C 6 ) Hydroxyalkyl radicals "or" (C) 1 -C 6 ) Aminoalkyl "means an amino group as defined above" (C 1 -C 6 ) An alkyl "group in which one or more hydrogen atoms are replaced by one or more hydroxyl (OH) or amino groups, respectively. Examples are hydroxymethyl and aminomethyl groups and the like.
The definition of aminoalkyl includes those which are substituted with one or more amino groups (-NR) 7 R 8 ) Substituted alkyl (i.e., (C) 1 -C 6 ) Alkyl "groups"). An example of aminoalkyl is mono-aminoalkyl such as R 7 R 8 N-(C 1 -C 6 ) An alkyl group.
Refer to R as defined above and below 7 And R is 8 When R is 7 And R is 8 When taken together with the nitrogen atom to which they are attached form a 4-6 membered heterocyclic residue (similar to R above 2 And R is 3 ) At least one other ring carbon atom in the heterocyclic residue is optionally replaced by at least one heteroatom (e.g. N, NH, S or O) and/or may bear an-oxo (=o) substituent. The heterocyclic residue may further optionally be substituted at any available point in the ring (i.e., at a carbon atom or at any heteroatom available for substitution). Substitution on a carbon atom includes spiro di-substitution as well as substitution on 2 adjacent carbon atoms, in both cases thus forming an additional 5-6 membered heterocyclic ring. Examples of such heterocyclic residues are 1-pyrrolidinyl, 1-piperidinyl, 1-piperazinyl, 4-morpholinyl, piperazin-4-yl-2-one, 4-methylpiperazin-1-yl, 7-methyl-4, 7-diazaspiro [2.5 ]]Octan-4-yl, (3 aR,6 aS) -5-cyclopropyl hexahydropyrrolo [3,4-c]Pyrrol-2 (1H) -yl), (1S, 4S) -5-cyclopropyl-2, 5-diazabicyclo [2.2.1]Heptane-2-yl, 3, 4-dihydro-2, 7-naphthyridin-2 (1H) -yl, 7, 8-dihydro-1, 6-naphthyridin-6 (5H) -yl, and the like.
The term "(C) 3 -C 10 ) Cycloalkyl radicals "like" (C) 3 -C 6 ) Cycloalkyl "means containing the indicated number of ring carbon atoms Saturated cyclic hydrocarbyl of the sub (including the corresponding spiro disubstituted divalent radicals). Examples include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and cycloheptyl and polycyclic ring systems such as adamantyl.
The term "(C) 2 -C 6 ) Alkenyl "means a straight or branched carbon chain having one or more conjugated or non-conjugated double bonds in cis or trans configuration, wherein the number of atoms is in the range of 2-6.
By analogy, the term "(C) 5 -C 7 ) Cycloalkenyl "means a cyclic hydrocarbon group containing 5 to 7 ring carbon atoms and one or two double bonds.
The term "(C) 2 -C 6 ) Alkynyl "means a straight or branched carbon chain having one or more triple bonds, wherein the number of atoms is in the range of 2-6.
The term "(C) 2 -C 6 ) Hydroxy alkynyl "means (C) as defined above 1 -C 6 ) Alkynyl "groups in which one or more hydrogen atoms are replaced by one or more hydroxyl (OH) groups.
The term "(C) 2 -C 6 ) Amino alkynyl "means (C) as defined above 1 -C 6 ) Alkynyl "groups in which one or more hydrogen atoms are replaced by one or more (-NR) 7 R 8 ) And (3) replacing groups.
The expression "aryl" denotes a monocyclic, bicyclic or tricyclic carbocyclic ring system having 6 to 20, preferably 6 to 15, ring atoms, wherein at least one ring is aromatic. The expression "heteroaryl" denotes a monocyclic, bicyclic or tricyclic ring system having 5 to 20, preferably 5 to 15 ring atoms, wherein at least one ring is aromatic and wherein at least one ring atom is a heteroatom (e.g. N, S or O).
Examples of aryl or heteroaryl monocyclic ring systems include, for example, phenyl, thienyl, pyrrolyl, pyrazolyl, imidazolyl, iso-Azolyl, (-) -and (II) radicals>Azolyl groupIsothiazolyl, thiazolyl, pyridyl, pyrimidinyl, pyrazinyl, triazinyl, furyl residues and the like.
Examples of aryl or heteroaryl bicyclic ring systems include naphthyl, biphenylene, purinyl, pteridinyl, pyrazolopyrimidinyl, benzotriazolyl, benzimidazolyl, quinolinyl, isoquinolinyl, indolyl, isoindolyl, benzothienyl, benzodioxanyl (benzodioxanyl), dihydrobenzodioxanyl, indenyl, dihydro-indenyl, dihydrobenzo [1,4 ]]Dioxahexenyl, benzothiazol-2-yl, dihydrobenzodioxepinyl, benzodioxepinylOxazinyl residues, and the like.
Examples of aryl or heteroaryl tricyclic ring systems include fluorenyl and benzofused derivatives of the foregoing heteroaryl bicyclic ring systems.
In a similar manner, the expressions "arylene" and "heteroarylene" denote divalent radicals such as phenylene, biphenylene and thiophenylene. Such groups are also commonly referred to as "arenediyl" or "heteroarenediyl" groups. For example, ortho-phenylene is also known as benzene-1, 2-diyl. The thienylene group is alternatively referred to as a thienylene group.
Derived expression "(C) 3 -C 6 ) Heterocyclyl "denotes a saturated or partially unsaturated monocyclic ring (C 3 -C 6 ) Cycloalkyl, wherein at least one ring carbon atom is replaced by at least one heteroatom (e.g. N, NH, S or O) or may bear an-oxo (=o) substituent. The heterocycloalkyl group (i.e., a heterocyclic residue or group) may be further optionally substituted at the available point of the ring (i.e., at a carbon atom or at a heteroatom that is available for substitution). Substitution on a carbon atom includes spiro di-substitution as well as substitution on 2 adjacent carbon atoms, in both cases thus forming an additional fused 5-6 membered heterocyclic ring. (C) 3 -C 6 ) Examples of heterocycloalkyl groups are represented by: oxetanyl, tetrahydrofuranyl, pyrrolidinyl, imidazolidinyl, thiazolidinyl, piperazinyl, piperidinyl, morpholinyl, timololA pinyl, dihydro-or tetrahydro-pyridinyl, tetrahydropyranyl, pyranyl, 2H-or 4H-pyranyl, dihydro-or tetrahydrofuranyl, dihydro-iso-Oxazolyl, pyrrolidin-2-one-yl, dihydropyrrol residues, and the like.
Examples of such heterocyclic residues are 1-pyrrolidinyl, 1-methyl-2-pyrrolidinyl, 1-piperidinyl, 1-piperazinyl, 4-morpholinyl, piperazin-4-yl-2-one, 4-methylpiperazin-1-yl, 1-methylpiperidin-4-yl, 4-methylpiperazin-1-yl-2-one, 7-methyl-2, 7-diazaspiro [3.5] non-2-yl, 2-methyl-2, 9-diazaspiro [5.5] undec-9-yl, 9-methyl-3, 9-diazaspiro [5.5] undec-3-yl and (3 aR,6 aS) -5-methyl-octahydropyrrolo [3,4-c ] pyrrol-2-yl.
The term "aryl (C) 1 -C 6 ) Alkyl "means an aryl ring attached to a straight or branched chain alkyl group wherein the number of carbon atoms of the component is in the range of 1-6, such as phenylmethyl (i.e., benzyl), phenylethyl or phenylpropyl.
Likewise, the term "heteroaryl (C 1 -C 6 ) Alkyl "means a heteroaryl ring attached to a straight or branched chain alkyl group wherein the number of carbon atoms of the component is in the range of 1-6, such as furanylmethyl.
The term "alkanoyl" means HC (O) -or alkylcarbonyl (e.g. (C) 1 -C 6 ) Alkyl C (O) -, where the radical "alkyl" has the meaning defined above. Examples include formyl, acetyl, propionyl, butyryl.
Likewise, "(C) 1 -C 6 ) Alkyl-sulfonyl "means" (C) 1 -C 6 ) alkyl-S (O) 2 A group wherein alkyl has the meaning defined above. (C) 1 -C 6 ) Examples of alkyl-sulfonyl are methylsulfonyl, ethylsulfonyl, propylsulfonyl, isopropylsulfonyl.
"aryl (C) 1 -C 6 ) Alkyl-sulfonyl "denotes an aryl group further substituted with an aryl group as defined above (C 1 -C 6 ) Alkyl-sulfonyl. Aryl (C) 1 -C 6 ) An example of an alkyl-sulfonyl group is a benzylsulfonyl group.
The term "carbamoyl" denotes a radical of formula-C (O) NR 7 R 8 Represented aminocarbonyl-derived groups, wherein R 7 And R is 8 As defined above, including ortho, vicinal, and spirodisubstituted derivatives. Examples are aminocarbonyl, methylaminocarbonyl, methoxyethylaminocarbonyl, piperazine-1-carbonyl, morpholine-N-carbonyl and N- (2- (dimethylamino) ethyl) aminocarbonyl, N- (2- (dimethylamino) ethyl) -N-methylaminocarbonyl, N- (3- (dimethylamino) propyl) -N-methylaminocarbonyl, 4-methylpiperazine-1-carbonyl, 4- (dimethylamino) piperidine-1-carbonyl, N- (2- (4-methylpiperazin-1-yl) ethyl) aminocarbonyl, (2-morpholino-ethyl) aminocarbonyl, N-methyl-N- (2-morpholino-ethyl) aminocarbonyl, N- (2- (piperidin-1-yl) ethyl) aminocarbonyl, N-methyl-N- (2- (piperidin-1-yl) ethyl) aminocarbonyl, N- (1-methylpiperidin-4-yl-methyl) aminocarbonyl, N-methyl-N- (1-methylpiperidin-4-yl) aminocarbonyl, 5-methyl octahydropyrrolo [3,4-c]Pyrrole-2 carbonyl.
The term "hydroxycarbonyl" denotes the terminal group HOC (O) -.
The term "(C) 1 -C 10 ) Alkoxy group OR (C) 1 -C 10 ) Alkoxy "analogous to" (C) 1 -C 6 ) Alkoxy group OR (C) 1 -C 6 ) Alkoxy "and the like represent a straight or branched hydrocarbon of the indicated number of carbons attached to the remainder of the molecule through an oxygen bridge. "(C) 1 -C 6 ) Alkylthio "means the hydrocarbon described above linked through a sulfur bridge.
Derived expression "(C) 1 -C 6 ) Haloalkoxy "or" (C) 1 -C 6 ) Haloalkoxy "means a haloalkyl group as defined above which is linked through an oxygen bridge. (C) 1 -C 6 ) An example of a haloalkoxy group is trifluoromethoxy.
By analogy, derived expressions "(C) 3 -C 6 ) Heterocyclyloxy "and" (C) 3 -C 6 ) Heterocycloalkyl (C) 1 -C 6 ) Alkoxy "represents a heterocycloalkyl and a chain (charged) heterocycloalkyl-alkoxy group, respectively, linked by an oxygen bridge. Examples are (piperidin-4-yl) oxy, 1-methylpiperidin-4-yl) oxy, 2- (piperidin-4-yl) ethoxy, 2- (1-methylpiperidin-4-yl) ethoxy and 2- (4-morpholino) ethoxy, respectively.
The derived expressions "aryloxy" and "aryl (C 1 -C 6 ) Alkoxy "analogous to" heteroaryloxy "and" heteroaryl (C 1 -C 6 ) Alkoxy "means aryl or heteroaryl and chain aryl-alkoxy or heteroaryl-alkoxy linked through an oxygen bridge. Examples of such groups are phenoxy and benzyloxy and pyridyloxy, respectively.
Also, derived expression "(C) 3 -C 6 ) Heterocycloalkyl- (C) 1 -C 6 ) Alkyl "and" (C) 3 -C 6 ) Cycloalkyl- (C) 1 -C 6 ) Alkyl "represents heterocycloalkyl and cycloalkyl groups as defined above attached to the rest of the molecule via alkyl of the indicated number of carbons, e.g. piperidin-4-yl-methyl, cyclohexylethyl.
Derived expression "(C) 1 -C 6 ) Alkoxy- (C) 1 -C 6 ) Alkyl "represents an alkoxy group as defined above attached to the rest of the molecule via an alkyl group of the indicated number of carbons, such as methoxymethyl.
Derived expression "(C) 1 -C 6 ) Alkoxycarbonyl "means an alkoxy group as defined above attached to the rest of the molecule via a carbonyl group, such as ethoxycarbonyl.
Other derived expressions such as "(C) 1 -C 6 ) Alkoxycarbonyl-amino "means via carbonyl followed by amino group (-NR) 7 (-) alkoxy as defined above attached to the rest of the molecule, e.g. tert-butoxy-carbonyl-amino-.
“(C 1 -C 6 ) Alkoxycarbonyl group (C) 3 -C 6 ) Heterocycloalkyl (C) 1 -C 6 ) Alkyl "means chain-formed (interrupted) in the stated order and attached to the remainder of the molecule via alkyl of the indicated number of carbonsAn alkoxycarbonyl heterocycloalkyl substituent. An example is (tert-butylpiperidine-1-carboxylate) -4-yl-methyl.
Derived expression "(C) 1 -C 6 ) Amino alkoxy "denotes an amino group as defined above (C 1 -C 6 ) Aminoalkyl groups such as (2- (dimethylamino) ethoxy.
Expression "(C) 1 -C 6 ) Hydroxyalkoxy "means a hydroxyalkyl group as defined above, e.g. a hydroxyethoxy group, attached to the remainder of the molecule through an oxygen bridge.
Derived expression "(C) 1 -C 6 ) Aminoalkylcarbamoyl "denotes quilt (C) 1 -C 6 ) Aminoalkyl substituted "carbamoyl" groups as defined above (i.e. -C (O) NR 7 R 8 Wherein for example R 8 Is (C) 1 -C 6 ) Aminoalkyl). One example is 2- (dimethylamino) ethylcarbamoyl.
The term "arylalkanoyl" means "aryl-carbonyl" (i.e., aryl C (O)) or arylalkyl-carbonyl [ i.e., aryl (C) 1 -C 6 ) Alkyl C (O) radicals]Wherein aryl and alkyl have the meanings defined above. Examples are represented by: benzoyl (i.e., phenylcarbonyl), phenylacetyl, phenylpropionyl, or phenylbutyryl residues. Similarly, "arylsulfonyl" means aryl S (O) 2 A group wherein aryl has the meaning defined above. An example is benzenesulfonyl.
The term "heteroarylsulfonyl" means heteroaryl S (O) 2 A group wherein heteroaryl has the meaning defined above. An example is pyridylsulfonyl.
As in the definitions provided above, the chain-forming substituents derive their definition from the constituent fragments, such as "(C) 3 -C 6 ) Cycloalkyl-carbonyl "," (C) 3 -C 6 ) Heterocycloalkyl-carbonyl "," heteroaryl-carbonyl "; represents a fragment as defined above linked to the rest of the molecule via a carbonyl group. Examples of such groups include cyclopropanecarbonyl, pyrrolidine-3-carbonyl, (pyridin-3-yl) carbonyl.
The expression "saturated, partially unsaturated or aromatic 5-or 6-membered cycloalkane-diyl, arylene-diyl or heterocycle-diyl" denotes a suitable disubstituted cycloalkane or heterocycle or aromatic residue having 5 or 6 elements, including 1,2-, 1, 3-or 1, 4-benzene-diyl; 2,3-, 3,4-, 4, 5-or 5, 6-pyridin-diyl; 3,4-, 4, 5-or 5, 6-pyridazin-diyl; 4, 5-or 5, 6-pyrimidin-diyl; 2, 3-pyrazinediyl; 2,3-, 3, 4-or 4, 5-thiophen-diyl/furan-diyl/pyrrole-diyl; 4, 5-imidazol-diyl-Oxazolediyl/thiazolediyl; 3, 4-or 4, 5-pyrazol-diyl/i->Azolediyl/isothiazolediyl, saturated or partially unsaturated analogs thereof, and the like. Other non-vicinal disubstituted residues (diradicals) are also included, such as 4, 6-pyrimidine-diyl and the like.
The expression "ring system" denotes mono-or bi-or polycyclic ring systems which may be saturated, partially unsaturated or unsaturated, such as aryl, (C) 3 -C 10 ) Cycloalkyl, (C) 3 -C 6 ) Heterocycloalkyl or heteroaryl.
The oxo moiety is represented by (O) as a substitute for other general representations (e.g., (=o)). Thus, in the manner of the general formula, carbonyl is preferably denoted herein as-C (O) -as a substitute for other common expressions such as-CO-, - (CO) -or-C (=o) -. In general, the bracketed group is a pendant group, is not included in the chain, and when deemed useful, brackets are used to help disambiguate the linear chemical formula; for example sulfonyl-SO 2 Possibly also denoted as-S (O) 2 To disambiguate for example with respect to sulfinyl-S (O) O-.
When a numerical index is used as in the statement "p is 0 or an integer of 1 to 3", the statement (value) "p is 0" means that substituent R is not present, that is, substituent R is not present on the ring.
Whenever a basic amino or quaternary ammonium group is present in the compound of formula I, a physiologically acceptable anion may be present selected from chloride, bromide, iodide, trifluoroacetate, formate, sulfate, phosphate, methanesulfonate, nitrate, maleate, acetate, citrate, fumarate, tartrate, oxalate, succinate, benzoate, p-toluenesulfonate, pamoate (pamoate) and naphthalenedisulfonate (naphthalene disulfonate). Likewise, in the presence of acidic groups such as COOH groups, corresponding physiological cation salts, including for example alkali metal or alkaline earth metal ions, may also be present.
The compounds of formula (I) may exist as optical stereoisomers when they contain one or more stereogenic centers.
In the case of the compounds according to the invention having at least one stereocenter, they may accordingly exist as enantiomers. Where the compounds of the invention have two or more stereocenters, they may additionally exist as diastereomers. It is to be understood that all such single enantiomers, diastereomers, and mixtures thereof in any ratio thereof, are included within the scope of the present invention. The absolute configuration (R) or (S) of the carbon with the stereocenter is specified based on the Cahn-Ingold-Prelog naming convention based on the priority of the groups.
When reported near the chemical name of a compound, "single stereoisomer", "single diastereomer" or "single enantiomer" indicates that the isomer is separated into single diastereomers or enantiomers (e.g., via chromatography), but the absolute configuration at the relevant stereocenter is not determined/specified.
The atropisomers result from hindered rotation about a single bond, where the spatial dystonia of rotation is high enough to allow separation of conformational isomers (Brigmann G et al, angew.Chemie int. Ed.44 (34), 5384-5427,2005.Doi:10.1002/anie. 200462661).
Oki atropisomers are defined as conformational isomers: which interconvert at a given temperature with a half-life of more than 1000 seconds (Oki M, topics in Stereochemistry, 14,1-82,1983).
Atropisomers differ from other chiral compounds in that in many cases they can be thermally balanced, whereas in other forms of chirality isomerisation is often only chemically possible.
Separation of atropisomers is possible by chiral resolution methods such as selective crystallization. In the atrop-enantioselective or atropselective synthesis, one atropisomer is formed at the cost of the other atropisomer. The synthesis of the anti-rotation selectivity can be carried out by using chiral auxiliary such as Corey Bakshi Shibata (CBS) catalyst, i.e. an asymmetric catalyst derived from proline; or by a scheme based on thermodynamic equilibrium where the isomerisation reaction favors one atropisomer over another.
Within the scope of the present invention are the racemic forms of the compounds of formula (I) as well as the individual atropisomers (substantially free of their corresponding enantiomers) and the stereoisomer-enriched mixtures of atropisomers.
The invention further relates to corresponding deuterated derivatives of the compounds of formula (I).
All preferred groups or embodiments described above and below in relation to the compounds of formula I may be combined with one another and applied mutatis mutandis.
The present invention relates to classes of compounds that function as inhibitors of Rho kinase (ROCK).
The class of compounds inhibit the activity or function of ROCK enzymes, and more specifically, they are inhibitors of ROCK-I and ROCK-II isoforms of Rho-related coiled coil forming protein kinase (ROCK). The present invention relates to compounds of formula (I) or pharmaceutically acceptable salts and solvates thereof
Wherein the method comprises the steps of
X 1 And X 2 Independently at each occurrence is a CH group or a nitrogen atom.
p is 0 or an integer of 1 to 3
Each R, when present, is halogen;
R 0 and R is 1 Independently selected from:
-H，
(C 1 -C 6 ) An alkyl group, a hydroxyl group,
(C 3 -C 10 ) A cycloalkyl group,
aryl, heteroaryl and (C) 3 -C 6 ) Heterocycloalkyl group
The aryl, heteroaryl and (C 3 -C 6 ) Each of the heterocycloalkyl groups is in turn optionally and independently substituted with one or more groups selected from:
A halogen atom,
-OH，
r, identical or different 2 And R is 3 Selected from:
-H，
(C 1 -C 6 ) An alkyl group, a hydroxyl group,
(C 1 -C 6 ) A haloalkyl group, a halogen atom,
(C 1 -C 6 ) A hydroxyalkyl group, a hydroxyl group,
(C 1 -C 6 ) An amino alkyl group,
(C 1 -C 6 ) Alkoxy (C) 1 -C 6 ) An alkyl group, a hydroxyl group,
(C 3 -C 10 ) A cycloalkyl group,
(C 3 -C 8 ) A heterocycloalkyl group, a heterocyclic ring-like group,
an aryl group,
a heteroaryl group, which is a group,
aryl (C) 1 -C 6 ) An alkyl group, a hydroxyl group,
heteroaryl (C) 1 -C 6 ) An alkyl group, a hydroxyl group,
(C 3 -C 8 ) Cycloalkyl (C) 1 -C 6 ) An alkyl group, a hydroxyl group,
(C 3 -C 8 ) Heterocycloalkyl- (C) 1 -C 6 ) An alkyl group, a hydroxyl group,
each of the aryl, heteroaryl, cycloalkyl, heterocycloalkyl groups is further optionally selected independently by one or moreSubstituted from the following groups: halogen, -CN, -OH, (C) 1 -C 8 ) Alkyl, (C) 3 -C 6 ) Cycloalkyl, (C) 1 -C 6 ) Haloalkyl, (C) 1 -C 10 ) Alkoxy, heterocycloalkyl, aryl (C) 1 -C 6 ) Alkyl, -C (O) NR 7 R 8 、(C 1 -C 6 ) Aminoalkyl group (C) 1 -C 6 ) Hydroxyalkyl (C) 1 -C 6 ) Alkoxy (C) 1 -C 6 ) Alkyl, (C) 3 -C 8 ) Cycloalkyl (C) 1 -C 6 ) An alkyl group; or (b)
As an alternative, R 2 And R is 3 Together with the nitrogen atom to which they are attached, form a mono-or bi-cyclic saturated or partially saturated heterocyclic residue, preferably a 4-6 membered monocyclic residue, at least one other ring carbon atom of which is optionally substituted with at least one other heteroatom independently selected from N, NH, S or O and/or may bear an-oxo (=o) substituent, said heterocyclic residue further optionally comprising spiro di-substitution and substitution at two ortho or vicinal atoms, thereby forming an additional 5-6 membered cyclic or heterocyclic, saturated, partially saturated or aromatic ring;
The heterocyclic residue is optionally further substituted with one or more groups selected from:
a halogen atom,
-OH，
-NR 7 R 8 ，
-CH 2 NR 7 R 8 ，
(C 1 -C 6 ) An alkyl group, a hydroxyl group,
(C 1 -C 6 ) An alkyl-sulfonyl group,
(C 1 -C 6 ) A haloalkyl group, a halogen atom,
(C 1 -C 6 ) A hydroxyalkyl group, a hydroxyl group,
(C 2 -C 6 ) An alkenyl group,
(C 2 -C 6 ) An alkynyl group, an amino group,
(C 2 -C 6 ) Hydroxy alkynyl，
(C 1 -C 6 ) Alkoxy (C) 1 -C 6 ) An alkyl group, a hydroxyl group,
(C 1 -C 6 ) An alkanoyl group, which is a fatty acid,
-C(O)NR 7 R 8 ，
(C 3 -C 6 ) Cycloalkyl-carbonyl group, the cycloalkyl-carbonyl group,
(C 3 -C 6 ) A heterocycloalkyl-carbonyl group, a heterocyclic ring,
aryl (C) 1 -C 6 ) An alkyl group, a hydroxyl group,
an aryl alkanoyl group,
an aryl sulfonyl group,
aryl (C) 1 -C 6 ) An alkyl-sulfonyl group,
heteroaryl (C) 1 -C 6 ) An alkyl group, a hydroxyl group,
a heteroaryl-carbonyl group, which is a group,
heteroaryl sulfonyl
A heteroaryloxy group, a heteroaryl group,
(C 3 -C 6 ) Cycloalkyl, including cycloalkyl-groups,
(C 3 -C 8 ) Cycloalkyl (C) 1 -C 6 ) Alkyl group
(C 3 -C 6 ) Heterocycloalkyl- (C) 1 -C 6 ) An alkyl group, a hydroxyl group,
aryl and heteroaryl groups
Each of the cycloalkyl, aryl and heteroaryl groups is further optionally substituted with halogen, -OH, (C) 1 -C 8 ) Alkyl, (C) 1 -C 6 ) Haloalkyl, (C) 1 -C 10 ) Alkoxy, (C) 1 -C 6 ) Alkylthio, (C) 1 -C 6 ) Aminoalkyl group (C) 1 -C 6 ) Amino alkoxy, -C (O) NR 7 R 8 、(C 1 -C 6 ) Alkyl-sulfonyl substitution;
R 4 and R is 5 Independently at each occurrence selected from
H，
(C 1 -C 6 ) An alkyl group, a hydroxyl group,
R 6 selected from-H, (C) 1 -C 6 ) Alkyl, (C) 1 -C 6 ) A haloalkyl group;
R 7 and R is 8 Independently at each occurrence selected from
H，
(C 1 -C 6 ) An alkyl group, a hydroxyl group,
(C 1 -C 6 ) A haloalkyl group, a halogen atom,
(C 1 -C 6 ) A hydroxyalkyl group, a hydroxyl group,
(C 1 -C 6 ) An amino alkyl group,
(C 1 -C 6 ) An alkoxy group, an amino group,
(C 1 -C 6 ) Alkoxy- (C) 1 -C 6 ) An alkyl group, a hydroxyl group,
(C 3 -C 6 ) Heterocycloalkyl- (C) 1 -C 6 ) An alkyl group, a hydroxyl group,
(C 3 -C 6 ) Cycloalkyl, aryl, heteroaryl and (C) 3 -C 6 ) A heterocycloalkyl group;
wherein the aryl, heteroaryl and (C 3 -C 6 ) Any of the heterocycloalkyl groups are in turn optionally and independently substituted with one or more groups selected from:
a halogen atom,
-OH，
(C 1 -C 6 ) An alkyl group.
In a preferred embodiment, the invention relates to a compound of formula (I) as defined above, represented by formula Ia, wherein X 1 And X 2 Is CH:
in a second preferred embodiment, the present invention relates to a compound of formula (I) as defined above, represented by formula Ic:
wherein the method comprises the steps of
X 3 is-O-or- (CH) 2 ) n -, where n is an integer selected from 1, 2 and 3, and
R 9 selected from:
-C(O)NR 7 R 8 and (C) 1 -C 6 ) A hydroxyalkyl group;
all other variables are as defined above.
Preferred in this embodiment are compounds of formula (Ic) as defined above or pharmaceutically acceptable salts and solvates thereof,
wherein the method comprises the steps of
X 1 Is CH or N, and X 2 Is a CH group;
p is 0 or an integer of 1 to 3
Each R, when present, is halogen;
R 0 is-H, and
R 1 is (C) 1 -C 6 ) An alkyl group, a hydroxyl group,
R 3 is a group of the formula-H,
R 4 and R is 5 Is all the same as the hydrogen atom in the hydrogen atom,
R 6 is-H;
R 9 is-C (O) NR 7 R 8 Wherein R is 7 Is H and R 8 Selected from H, (C) 1 -C 6 ) Alkyl, (C) 1 -C 6 ) Hydroxyalkyl radical and (C) 1 -C 6 ) Alkoxy (C) 1 -C 6 ) An alkyl group.
A preferred group of compounds according to the invention are compounds of formula (I) and pharmaceutically acceptable salts and solvates thereof
Wherein the method comprises the steps of
X 1 And X 2 Independently at each occurrence a CH group or a nitrogen atom;
p is 0 or an integer from 1 to 3;
each R, when present, is fluorine;
R 0 is-H, and R 1 Is a methyl group, and is preferably a methyl group,
R 3 is-H or methyl, and R 2 Independently selected from:
-H
a methyl group,
(C 3 -C 10 ) Cycloalkyl which is cyclohexyl, cyclobutyl or cyclopentyl,
(C 3 -C 8 ) Heterocycloalkyl which is piperidinyl, pyranyl or pyrrolidinyl,
each of the cycloalkyl, heterocycloalkyl is further optionally substituted with one or more groups independently selected from: (C) 1 -C 8 ) Alkyl, which is methyl, ethyl, isobutyl, tert-butyl, 1-isopropyl; (C) 3 -C 6 ) Cycloalkyl which is cyclopropyl or cyclobutyl, (C) 1 -C 6 ) Haloalkyl, which is fluoropropyl, heterocycloalkyl, which is oxetanyl or tetrahydrofuranyl; -C (O) NR 7 R 8 It is aminocarbonyl, methylaminocarbonyl, methoxyethylaminocarbonyl or hydroxyethylaminocarbonyl; (C) 1 -C 6 ) Hydroxyalkyl, which is hydroxyethyl, hydroxymethyl; (C) 1 -C 6 ) Alkoxy (C) 1 -C 6 ) Alkyl, which is methoxyethyl, (C) 3 -C 8 ) Cycloalkyl (C) 1 -C 6 ) Alkyl, which is cyclopropylmethyl; or alternatively
In the alternative, R 2 And R is 3 Together with the nitrogen atom to which they are attached, form a monocyclic group which is piperidin-N-yl, pyrrolidin-N-yl, piperazin-N-yl;
Or a bicyclic group which is 4, 7-diazaspiro [2.5] oct-4-yl, (3 ar,6 as) -5-cyclopropyl-hexahydropyrrolo [3,4-c ] pyrrol-2 (1H) -yl), (1 s,4 s) -5-cyclopropyl-2, 5-diazabicyclo [2.2.1] heptan-2-yl, 3, 4-dihydro-2, 7-naphthyridin-2 (1H) -yl, 5, 8-dihydropyrido [3,4-d ] pyrimidin-7 (6H) -yl, 6, 7-dihydrothiazolo [5,4-c ] pyridin-5 (4H) -yl, 7, 8-dihydro-1, 6-naphthyridin-6 (5H) -yl;
R 4 、R 5 and R is 6 is-H.
The invention also provides a pharmaceutical composition comprising a compound of formula I, or a pharmaceutically acceptable salt thereof, alone or in combination with one or more other active ingredients, in admixture with one or more pharmaceutically acceptable carriers or excipients.
In one aspect, the present invention provides a compound of formula (I) for use as a medicament.
In another aspect, the invention provides the use of compound (I) or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for the treatment of a disorder associated with the ROCK enzyme mechanism, in particular for the treatment of a disorder such as a pulmonary disease.
In particular, the present invention provides compounds of formula (I) for use in the prevention and/or treatment of a pulmonary disease selected from asthma, chronic obstructive pulmonary disease COPD, idiopathic Pulmonary Fibrosis (IPF), pulmonary Hypertension (PH) and in particular Pulmonary Arterial Hypertension (PAH).
Furthermore, the present invention provides a method for preventing and/or treating disorders associated with the ROCK enzyme mechanism, comprising administering to a patient in need of such treatment a therapeutically effective amount of a compound of the present invention.
In particular, the invention provides a method for the prevention and/or treatment of a disorder, wherein the disorder is asthma, chronic obstructive pulmonary disease COPD Idiopathic Pulmonary Fibrosis (IPF), pulmonary Hypertension (PH) and especially Pulmonary Arterial Hypertension (PAH).
According to particular embodiments, the present invention provides the compounds listed in the following table, as well as pharmaceutically acceptable salts thereof.
The compounds of the present invention, including all of the compounds listed above, can be prepared from readily available starting materials using the general methods and procedures described below or by using slightly modified methods readily available to one of ordinary skill in the art. Although specific embodiments of the invention have been illustrated or described herein, those of ordinary skill in the art will recognize that all embodiments or aspects of the invention may be prepared using the methods described herein or by using other known methods, reagents, and starting materials. When typical or preferred process conditions (process conditions) (i.e., reaction temperature, time, molar ratio of reactants, solvent, pressure, etc.) are given, other process conditions may also be used unless otherwise indicated. Although the optimum reaction conditions may vary with the particular reactants or solvents used, such conditions can be readily determined by one skilled in the art by routine optimization procedures.
Thus, the preparation methods described below and reported in the schemes below should not be construed as limiting the scope of synthetic methods that can be used to prepare the compounds of the invention.
In some cases, a step is required to mask or protect sensitive or reactive moieties, and commonly known Protecting Groups (PG) may be employed according to general chemistry (Protective group in organic syntheses, 3 rd edition T.W.Greene, P.G.M.Wuts).
The preparation methods described below and reported in the schemes below should not be construed as limiting the scope of synthetic methods that can be used to prepare the compounds of the invention.
The compounds of formula I, including all compounds listed above, can generally be prepared according to the procedures shown in the schemes below. Where specific details or steps differ from the general approach, they have been described in particular embodiments and/or in additional approaches.
The compounds of formula I contain at least one stereocenter, which is marked with an asterisk in the figure below.
By means of enantiomerically pure (enantiomerically pure) starting materials and intermediates, enantiomerically pure compounds can be prepared according to the reaction described below. By means of enantiomerically pure intermediates IV and XII found in the scheme below, the carrying-NR can be accomplished 4 R 5 The preparation of enantiomerically pure compounds of formula I on the carbon (which is marked with an asterisk in the above figure). These intermediates may be commercially available or readily produced from commercial sources.
In another method, enantiomerically pure compounds can be prepared from the corresponding racemates by means of chiral chromatography. Whenever two or more stereocenters are present in a compound of formula I, the structure is characterized by different stereoisomers. The stereochemically pure compounds can be obtained as follows: by chiral separation from a mixture of diastereomers, or by chromatographic separation of the diastereomers followed by further chiral separation into the individual stereoisomers.
According to scheme 1 as described below, compounds of formula I may be prepared wherein R 5 Is H. Scheme 1 provides at least one non-limiting synthetic route for preparing examples 1-39, 41 and 43-47.
Typical Protecting Groups (PG) for protecting NH of 5-membered ring of bicyclic intermediate II 1 ) Can be 2- [ (trimethylsilyl) ethoxy]Methyl (SEM), 4-toluenesulfonyl (Ts) and p-methoxybenzyl (PMB), and the use of other protecting groups is not limited in any way. From the corresponding intermediate II and the appropriate PG 1 Reagents introduced, e.g. Ts-Cl (tosyl chloride), SEM-Cl ([ 2- (trimethylsilyl) ethoxy)]Methyl chloride) or PMB-Br (p-methoxybenzyl bromide) can be used to prepare intermediate III. The reaction between the components may be carried out in a polar organic solvent such as DMF, DCM or MeCN at room temperature or below in the presence of a base such as NaH or DIPEA.
The carboxylic acid of intermediate IV may be PG 2 Suitably protected as esters(e.g., as methyl ester), and using PG for amino groups 3 (e.g., boc group) is protected as a carbamate. These transformations can be effected using generally known methods starting from unprotected tyrosine-like derivatives.
Intermediate V can be obtained from intermediates III and IV by palladium catalyzed O-arylation. For example, the reaction may be accomplished as follows: in a suitable organic solvent such as toluene or THF in an inorganic base such as K 2 CO 3 In the presence of a suitable palladium catalytic system such as Pd 2 dba 3 XPhos or another palladium source/phosphine-based ligand, the aryl halide intermediate III and the phenol derivative IV are reacted at elevated temperature (about 100 ℃) for several hours.
In a different method, intermediate V can be obtained by a two-step synthesis starting from intermediate VIII. In a high boiling point organic solvent such as DMSO, at a temperature equal to or higher than 100deg.C and in an inorganic base such as K 2 CO 3 In the presence, the substitution of the nitro group of intermediate VIII by phenol of intermediate IV (ipso-substitution) can be achieved to yield intermediate VII. Intermediate VII can be converted to intermediate V as follows: chlorine atoms are removed by heterogeneous palladium-catalyzed hydrogenation (hetergeneous palladium catalyzed hydrogenation) by reacting VII in the presence of Pd/C and an organic base such as TEA under a hydrogen atmosphere. Intermediate VIII can be prepared analogously to intermediate III from the corresponding unprotected heterocycle as described above.
Using inorganic bases such as LiOH or Ba (OH) 2 PG can be removed from intermediate V by hydrolysis in an organic solvent such as THF and/or a mixture of methanol and water, typically at room temperature, for a period of from 1h to overnight 2 (when PG 2 Methyl) to give intermediate VI without affecting other Protection (PG) 1 SEM, ts or PMB and PG 3 Boc). In some cases, hydrolysis may be effected at a temperature equal to or higher than 50℃for ease of synthesis, and may result in simultaneous PG 1 Cleavage to yield intermediate VIa. Intermediate VIa may be used in a similar manner as intermediate VI.
Under suitable amide coupling reaction conditions, a reaction between intermediate VI (or VIa) and intermediate IX can be effected to yield intermediate X (or Xa). For example, intermediates VI (or VIa) and IX may be reacted with an organic base such as DIPEA or TEA in the presence of an activator such as COMU or HATU in a suitable organic solvent such as DCM or DMF and for a period of time ranging from a few hours to overnight, typically at a temperature around room temperature.
Alternatively, intermediate X may be prepared from intermediate XI and intermediate III by palladium-catalyzed O-arylation in a similar manner as described above for the preparation of intermediate V. In some cases, X in intermediate X 1 When =n, by dissolving in a polar organic solvent such as DMSO in an inorganic base such as K 2 CO 3 Heating intermediate III (wherein X 1 =n) and intermediate XI, O-arylation can be performed under alternative conditions.
In an alternative process, intermediate X can be prepared in a similar manner to that described above with respect to the reaction of intermediate VIII and intermediate IV, by means of the substitution in situ from intermediate XI and intermediate VIII, followed by hydrogenation to produce intermediate V as with respect to intermediate VII.
Intermediate XI can be obtained by amide coupling of intermediate XII with intermediate IX in a similar manner as described above for the preparation of intermediate X from intermediates VI and IX.
PG can be achieved stepwise or in parallel, depending on the cutting conditions used (Protective group in organic syntheses, 3 rd edition T.W.Greene, P.G.M.Wuts) 1 And PG 3 From intermediate X (or Xa, which carries PG alone) 3 ) To produce a compound of formula I (wherein R 5 Is H). For example, acidic cleavage using a mixture of TFA in an organic solvent such as DCM may deprotect Boc and PMB, while SEM may require additional treatment in concentrated methanolic ammonia or LiOH. Tosyl (Ts) groups can be hydrolyzed in a solution of an inorganic base such as LiOH in water/methanol at a temperature equal to or above 50 ℃.
In the deprotection of PG 1 And PG 3 R of the (elabelate) intermediate X can be prepared with further care before 2 Or R is 3 Substituents to produce compounds of formula I. For example, asFruit R 2 Is a methyl 1-cyclohexanecarboxylate residue and R 3 Is H, R 2 Can be easily converted to the corresponding amide in a two-step process comprising hydrolysis of the methyl ester and amide coupling.
Thus, the present invention also relates to a process for preparing a compound of formula I, which comprises reacting a compound of formula VI with a compound of formula IX under amine coupling conditions, followed by removal of the protecting group.
The invention also relates to compounds of formula VI.
Wherein PG 1 And PG 3 Is a protecting group.
Preferred are compounds of formula VI wherein X 1 、X 2 、R、R 0 、R 1 、R 4 、R 5 、R 6 And p is as defined according to the first embodiment of formula (I) or preferably according to the preferred embodiment of formula (Ib) or (Ic).
The invention also relates to the use of the compounds of formula VI as intermediates in the preparation of the compounds of formula I.
The invention also relates to the use of process VI as described above as an intermediate in the preparation of compounds of formula I.
In another method, a compound of formula I (wherein R is 5 ＝H，R 1 Is alkyl or cycloalkyl) to provide at least one non-limiting synthetic pathway for the preparation of examples 40 and 42.
Intermediate V (wherein R) can be obtained by electrophilic halogenation with the corresponding NXS (N-halosuccinimide, X: cl, br or I) achieved in an organic solvent such as MeCN and maintained at a temperature of around room temperature for several hours 1 H) to intermediate XIII.
R is introduced by cross-coupling via metal catalysis, such as palladium catalyzed Suzuki cross-coupling or other cross-couplings described in the following references (Strategic application of named reactions in organic synthesis, L.Kurti, B.Czako, code 2005) 1 A group which can convert intermediate XIII to intermediate V (wherein R 1 Is alkyl or cycloalkyl). For example, the insertion of R may be performed as follows 1 Is a Suzuki coupling of (a): in a mixture of water/organic solvents such as DMF or THF in a Pd catalyst such as PdCl 2 (dppf) 2 Intermediate XIII and the appropriate boric acid or pinacolate derivative are reacted with an inorganic base such as an alkaline carbonate or phosphate in the presence of DCM addition compound or PdXPhos G2 at a temperature of about 80 ℃ to 100 ℃ or higher for several hours. Intermediate V (wherein R 1 Is alkyl or cycloalkyl) to intermediate X (wherein R 1 Is alkyl or cycloalkyl) by a two-step process comprising the use of a process for converting intermediate V to intermediate VI (removal of PG) in accordance with scheme 1 2 ) And then intermediate VI is converted to intermediate X (removal of PG 2 ) The same reaction has been described to remove PG 2 And amide coupling.
In a different approach, intermediate V is prepared by the procedure described above with respect to intermediate XIII (wherein R 1 Is alkyl or cycloalkyl) can be obtained from intermediate XIV (where R 1 Is alkyl or cycloalkyl). To be as described above with respect to V (wherein R 1 Is H) to XIII in a manner analogous to that already described, it is possible to obtain the desired starting material from intermediate X (wherein R 1 H) to give intermediate XIV.
As already described in scheme 1, intermediate X (wherein R 1 Is alkyl or cycloalkyl) to a compound of formula I (wherein R 5 ＝H，R 1 Alkyl or cycloalkyl). Halogenated compounds
The compounds of the invention are inhibitors of kinase activity, in particular Rho-kinase activity. In general, compounds that are ROCK inhibitors are useful in the treatment of a number of disorders associated with the mechanism of ROCK enzymes.
In one embodiment, disorders treatable by the compounds of the invention include glaucoma, inflammatory Bowel Disease (IBD), and pulmonary diseases selected from asthma, chronic Obstructive Pulmonary Disease (COPD), interstitial lung diseases such as Idiopathic Pulmonary Fibrosis (IPF) and Pulmonary Arterial Hypertension (PAH).
In another embodiment, the disorder treatable by the compounds of the invention is selected from asthma, chronic Obstructive Pulmonary Disease (COPD) and interstitial lung diseases such as Idiopathic Pulmonary Fibrosis (IPF) and Pulmonary Arterial Hypertension (PAH).
In another embodiment, the disorder is selected from the group consisting of Idiopathic Pulmonary Fibrosis (IPF) and Pulmonary Arterial Hypertension (PAH).
The methods of treatment of the present invention comprise administering to a patient in need thereof a safe and effective amount of a compound of formula (I) or a pharmaceutically acceptable salt thereof. As used herein, a "safe and effective amount" with respect to a compound of formula (I) or a pharmaceutically acceptable salt or other pharmaceutically active agent thereof refers to the amount of such compound: it is sufficient to treat the patient's condition, but low enough to avoid serious side effects, although it can be routinely determined by the skilled artisan. The compound of formula (I), or a pharmaceutically acceptable salt thereof, may be administered at one time or according to a dosing regimen (dosing regimen) in which several doses are administered at varying time intervals over a given period. Typical daily dosages may vary depending upon the particular route of administration selected.
The invention also provides pharmaceutical compositions of compounds of formula (I) in admixture with one or more pharmaceutically acceptable carriers or excipients, such as those described in Remington's Pharmaceutical Sciences Handbook, XVII Ed., mack pub, n.y., u.s.a.
The compounds of the present invention and their pharmaceutical compositions may be administered according to patient needs, for example, orally, nasally, parenterally (subcutaneously, intravenously, intramuscularly, intrasternally and by infusion), by inhalation, rectally, vaginally, topically (local), transdermally and by ocular administration.
Various solid oral dosage forms may be used to administer the compounds of the present invention, including the following solid dosage forms: tablets, soft capsules (gelcaps), capsules, caplets (caplets), granules, lozenges and self-contained powders (bulk powders). The compounds of the present invention may be administered alone or in combination with various pharmaceutically acceptable carriers, diluents (such as sucrose, mannitol, lactose, starch) and known excipients including suspending agents, solubilizers, buffers, binders, disintegrants, preservatives, colorants, flavoring agents, lubricants and the like. Timed release capsules, tablets and gels are also advantageous.
Various liquid oral dosage forms can also be used for administration of the compounds of the present invention, including aqueous and nonaqueous solutions, emulsions, suspensions, syrups and elixirs. Such dosage forms may also contain suitable known inert diluents such as water and suitable known excipients such as preservatives, wetting agents, sweeteners, flavoring agents, and agents for emulsifying and/or suspending the compounds of the present invention. For example, the compounds of the invention may be injected intravenously in the form of isotonic sterile solutions. Other formulations are also possible.
Suppositories for rectal administration of the compounds of the invention can be prepared by mixing the compounds with suitable excipients such as cocoa butter, salicylates and polyethylene glycols.
Formulations for vaginal administration may be in the form of creams, gels, pastes, foams or sprays containing in addition to the active ingredient such carriers as are also known in the art to be appropriate.
For topical administration, the pharmaceutical composition may be in the form of creams, ointments, liniments, lotions, emulsions, suspensions, gels, solutions, pastes, powders, sprays and drops suitable for application to the skin, eye, ear or nose. Topical administration may also include transdermal administration by means such as transdermal patches.
For the treatment of respiratory diseases, the compounds according to the invention are preferably administered by inhalation.
Inhalable formulations include inhalable powders, metered dose aerosols containing a propellant, or inhalable formulations without a propellant.
For administration as a dry powder, single or multi-dose inhalers known from the prior art can be used. In this case, the powder may be filled in gelatin, plastic or other capsules, cartridges or blister packs, or in a reservoir.
Diluents or carriers that are generally non-toxic and chemically inert to the compounds of the invention (e.g., lactose) or any other additives suitable for improving the respirable fraction (respirable fraction) may be added to the powdered compounds of the invention.
Inhalation aerosols comprising a propellant gas (e.g., a hydrofluoroalkane) may comprise the compounds of the invention in solution or dispersion. Propellant-driven formulations may also contain other ingredients, such as co-solvents, stabilizers or optionally other excipients.
Propellant-free inhalable formulations comprising the compounds of the invention may be in the form of solutions or suspensions in aqueous, alcoholic or hydroalcoholic media, and they may be sprayed or sonicated by spraying or by soft mist sprayers known from the prior art, such asTo be delivered.
The compounds of the invention may be administered as the sole active agent, or in combination with other pharmaceutically active ingredients (i.e. as co-therapeutic agents administered in fixed dose combinations or in combination therapy of separately formulated active ingredients) selected from the group consisting of: organic nitrate (organic nitrate) and NO donor; NO inhaled; a stimulus of soluble guanylate cyclase (sGC); agonists of the prostacyclin analog PGI2 and prostacyclin receptor; compounds that inhibit degradation of cyclic guanylate (cGMP) and/or cyclic adenosine monophosphate (cAMP), such as inhibitors of Phosphodiesterases (PDE) 1, 2, 3, 4 and/or 5, in particular PDE 5 inhibitors; human neutrophil elastase inhibitors; compounds that inhibit the signal transduction cascade, such as tyrosine kinase and/or serine/threonine kinase inhibitors; antithrombotics, such as platelet aggregation inhibitors, anticoagulants or fibrinolytic substances; active substances for lowering blood pressure, such as calcium antagonists, angiotensin II antagonists, ACE inhibitors, endothelin antagonists, renin inhibitors, aldosterone synthase inhibitors, alpha receptor blockers, beta receptor blockers and mineralocorticoid receptor antagonists; neutral endopeptidase inhibitors; a penetrant; ENaC blockers; anti-inflammatory agents, including corticosteroids and antagonists of chemokine receptors; antihistamines; antitussive; antibiotics such as macrolides and dnase pharmaceutical substances and selective cleavage agents such as recombinant human deoxyribonuclease I (rhdnase); agents that inhibit ALK5 and/or ALK4 phosphorylation of Smad2 and Smad 3; tryptophan hydrolase 1 (TPH 1) inhibitors and multi-kinase inhibitors.
In a preferred embodiment, the compounds of the invention are administered in combination with the following agents: phosphodiesterase V such as sildenafil, vardenafil and tadalafil; organic nitrates and NO donors (e.g. sodium nitroprusside, nitroglycerin, isosorbide mononitrate, isosorbide nitrate, molsidomine or SIN-1 and inhaled NO); synthetic prostacyclin analogs PGI2 such as iloprost, treprostinil, epoprostenol, and beraprost; agonists of the prostacyclin receptor such as selexiptag and compounds of WO 2012/00759; stimuli of soluble guanylate cyclase (sGC) such as riocidine and tyrosine kinases such as imatinib, sorafenib and nilotinib and endothelin antagonists (e.g., ma Xiteng (macitentan), bosentan, sitaxsentan and ambrisentan).
The dosage of the compounds of the invention will depend on a variety of factors including the particular disease to be treated, the severity of the symptoms, the route of administration, the frequency of dosage intervals, the particular compound used, the potency of the compound, the toxicological profile and the pharmacokinetic profile.
Advantageously, the compound of formula (I) may be administered at a dose of, for example, 0.001 to 1000 mg/day, preferably 0.1 to 500 mg/day.
When the compounds of formula (I) are administered by the inhaled route, they are preferably administered in a dose comprised between 0.001 and 500 mg/day, preferably between 0.1 and 100 mg/day.
Pharmaceutical compositions comprising the compounds of the invention are suitable for administration by inhalation, such as inhalable powders, metered dose aerosols containing a propellant or inhalable formulations free of a propellant.
The invention also relates to a device comprising a pharmaceutical composition comprising a compound according to the invention, which device may be a single-or multi-dose dry powder inhaler, a metered dose inhaler and a soft mist (soft mist) nebulizer.
The following examples illustrate the invention in more detail.
Preparation of intermediates and examples
Details of general experiments
Purification by chromatography indicates usePurification by company purification system or Biotage SP1 purification system. In the case of purification of the product using a Si column (cartridge), this means +.>A pre-packed polypropylene column (column) containing a polymer having an average size of 50 μm and a nominal +.>Irregular particles of porosity. Fractions containing the desired product (identified by TLC and/or LCMS analysis) were combined and concentrated in vacuo. In the case of using an SCX-2 column, 'SCX-2 column' means +.A strong cation exchange adsorbent containing un-end capped propylsulfonic acid functionalized silica is represented by +. >A pre-packed polypropylene column. After HPLC was used for purification (purification by MDAP) Fractions containing the desired product (identified by TLC and/or LCMS analysis) were pooled and the solvent was removed using a Biotage EV10 evaporator. Alternatively, the combined product fractions are lyophilized.
NMR spectra were obtained on a Varian Unity Inova spectrometer (with a 5mm inverted detection triple resonance probe running at 400 MHz), or on a Bruker Avance DRX 400 spectrometer (with a 5mm inverted detection triple resonance TXI probe running at 400 MHz), or on a Bruker Avance DPX 300 spectrometer (with a standard 5mm double frequency probe running at 300 MHz), or on a Bruker Fourier 300 spectrometer (with a 5mm double probe running at 300 MHz), or on a Bruker AVANCE III HD spectrometer (with a 5mm probe running at 600 MHz). Migration is given in ppm relative to tetramethylsilane.
LCMS method 1
The acquisition UPLC (binary Pump/PDA detector) +ZQ Mass spectrometer with C18-reverse phase column (ACQUITY UPLC BEH C1.7 μm,100×2.1 mm) maintained at 40℃eluting with A: water+0.1% formic acid; meCN+0.1% formic acid.
Gradient:
gradient-time Flow rate (mL/min) ％A ％B
0.00 0.4 95 5
0.40 0.4 95 5
6.00 0.4 5 95
6.80 0.4 5 95
7.00 0.4 95 5
8.00 0.4 95 5
detection-MS, UV PDA
MS ionization method-electrospray (positive/negative ions).
LCMS method 2
Acquity i-Class (quad pump/PDA detector) +Quattro Micro Mass Spectrometry with C18-reverse phase column (ACQUITY UPLC BEH C18 1.7 μm,100×2.1 mm) maintained at 40 ℃ with A: water+0.1% formic acid; meCN+0.1% formic acid.
Gradient:
detection-MS, UV PDA
MS ionization method-electrospray (positive/negative ions).
LCMS method 3
Acquity H-Class (quaternary pump/PDA detector) + QDa Mass spectrometer with C18-reverse phase column (Acquity UPLC CSH C18 1.7 μm, 50X 2.1 mm) maintained at 40℃eluting with A: water +0.1% formic acid; b: mecn+0.1% formic acid.
Gradient:
gradient-time Flow rate (mL/min) ％A ％B
0.00 1.0 97 3
4.00 1.0 1 99
4.4 1.0 1 99
4.5 1.0 97 3
5.0 1.0 97 3
detection-MS, UV PDA
MS ionization method-electrospray (positive/negative ions).
LCMS method 4
UPLC+Waters DAD+Waters SQD2, single quadrupole UPLC-MS with C18-reverse phase column (Acquity UPLC BEH Shield RP 18.7 μm 100X2.1 mm), elution using A: water containing 10mM ammonium bicarbonate; b: meCN.
Gradient:
detection-MS, UV PDA
MS ionization method-electrospray (positive/negative ions).
LCMS method 5UPLC+Waters DAD+Waters SQD2, single quadrupole UPLC-MS with C18-reverse phase column (Acquity UPLC BEH Shield RP 18.7 μm 100x2.1 mM), eluting with A: water containing 10mM ammonium bicarbonate; and B is MeCN.
Gradient:
gradient-time Flow rate (mL/min) ％A ％B
0.0 0.4 95 5
0.4 0.4 95 5
6.0 0.4 5 95
6.8 0.4 5 95
7.0 0.4 95 5
8.0 0.4 95 5
detection-MS, UV PDA
MS ionization method-electrospray (positive/negative ions).
LCMS method 6
UPLC+Waters DAD+Waters SQD2, single quadrupole UPLC-MS with C18-reverse phase column (Acquity UPLC HSS C18.8 μm 100x2.1 mm), eluting with A: water containing 0.1% formic acid; and B, meCN containing 0.1% formic acid.
Gradient:
detection-MS, UV PDA
MS ionization method-electrospray (positive/negative ions).
LCMS method 7
Acquity H-Class (quaternary pump/PDA detector) + QDa Mass spectrometer with C18-reverse phase column maintained at 50deg.C (Acquity BEH 1.7 μm, 50X2.1 mm), eluting with A: water+0.1% formic acid; meCN+0.1% formic acid.
Gradient:
gradient-time Flow rate (mL/min) ％A ％B
0.00 1 97 3
1.50 1 1 99
1.90 1 1 99
2.00 1 97 3
2.50 1 97 3
detection-MS, UV PDA
MS ionization method-electrospray (positive/negative ions).
LCMS method 8
UPLC+Waters DAD+Waters SQD2, single quadrupole UPLC-MS with C18 reverse phase column (Acquity UPLC HSS C18.8 μm 100×2.1 mm), eluting with A: water containing 0.1% formic acid; and B, meCN containing 0.1% formic acid.
Gradient:
gradient-time Flow rate (mL/min) ％A ％B
0.00 0.4 95 5
0.40 0.4 95 5
6.00 0.4 5 95
6.80 0.4 5 95
7.0 0.4 95 5
8.0 0.4 95 5
detection-MS, UV PDA
MS ionization method-electrospray (positive/negative ions).
LCMS method 9
Acquity H-Class (quaternary pump/PDA detector) + QDa Mass spectrometer with C18-reverse phase column (Acquity UPLC CSH C18 1.7 μm, 50X 2.1 mm) maintained at 40℃eluting with A: water+0.1% formic acid; meCN+0.1% formic acid.
Gradient:
gradient-time Flow rate (mL/min) ％A ％B
0.0 1.0 97 3
1.5 1.0 1 99
1.9 1.0 1 99
2.0 1.0 97 3
2.5 1.0 97 3
detection-MS, UV PDA
MS ionization method-electrospray (positive/negative ions).
MDAP method
Agilent Technologies 1260 Infinicity purification System with column maintained at room temperature and flow rate of 20 ml/min. The column, eluent and gradient are illustrated in the respective experimental descriptions.
SFC method
Supercritical Fluid Chromatography (SFC) was performed using a Waters Thar Prep100 preparative SFC system (P200 CO2 pump, 2545 regulated pump, 2998 uv/vis light detector, 2767 liquid processor with Stacked Injection Module) or Waters Thar Investigator semi-preparative system (Waters Fluid Delivery Module,2998 uv/vis light detector, waters Fraction Collection Module). The columns and isocratic methods used are indicated for each compound and the single enantiomer is analyzed using the methods given. In order to achieve the desired% ee purity, some compounds may have undergone a second purification process.
Abbreviations used：
Boc=tert-butoxycarbonyl; COMU (1-cyano-2-ethoxy-2-oxoethyleneaminooxy) -dimethylamino-morpholino carbonHexafluorophosphate; DCE = 1, 2-dichloroethane; DCM = dichloromethane; DEA = diethylamine; DIPEA = diisopropylethylamine; DMF = N, N-dimethylformamide; DMSO = dimethyl sulfoxide; h=small When in use; hatu= (1- [ bis (dimethylamino) methylene ]]-1H-1,2, 3-triazolo [4,5-b]Pyridine->3-oxide hexafluorophosphate); HPLC = high performance liquid chromatography; ims=industrial methylated spirits; LCMS = liquid chromatography-mass spectrometry; MDAP = mass directed auto-purification; meCN = acetonitrile; NIS = N-iodosuccinimide; pd (Pd) 2 (dba) 3 =tris (dibenzylideneacetone) dipalladium (0); pd (dppf) Cl 2 DCM = bis (diphenylphosphino) ferrocene]Palladium (II) dichloride, forming a complex with dichloromethane; pd Xphos g2=chloro (2-dicyclohexylphosphino-2 ',4',6 '-triisopropyl-1, 1' -biphenyl) [2- (2 '-amino-1, 1' -biphenyl)]Palladium (II); rt = retention time; RT = room temperature; SCX = strong cation exchange; SFC = supercritical fluid chromatography; TEA = triethylamine; TFA = trifluoroacetic acid; THF = tetrahydrofuran; xphos=2-dicyclohexylphosphino-2 ',4',6' -triisopropylbiphenyl.
In the following operations, some starting materials are identified by an "intermediate" or "example" number with a step number indicating. This is provided only to assist the skilled chemist.
By "similar" or "similar" operations is meant that such operations may involve minor variations, such as reaction temperature, reagent/solvent amounts, reaction time, work-up conditions, or chromatographic purification conditions.
Where indicated, the stereochemistry of the compounds in the examples are specified on the following assumptions: the absolute configuration at the resolved stereocenter of the starting material is maintained throughout any subsequent reaction conditions.
Ee% (enantiomeric excess) is measured by readily available chiral LC or SFC methods, e.g., as reported with respect to example 8. This method should be regarded as an example of an analytical method to be used for determining ee%.
Unless otherwise indicated, in the case where absolute configuration (R) or (S) is reported in the compound name, ee% must be regarded as equal to or greater than 90%. For those embodiments having less than 90% by ee measurement values, the exact values are reported. In the case where the measured values of ee% have not been determined, they are marked as n.d. (not determined).
Example 1
Step A
(S) -2-amino-3- (3-fluoro-4-hydroxyphenyl) propionic acid methyl ester hydrochloride (intermediate 1A-a)
Thionyl chloride (36.6 mL,0.5 mol) was added dropwise to cold methanol (200 mL) with stirring at 0deg.C. The mixture was stirred cold at this temperature for 15min, then 3-fluoro-L-tyrosine (20 g,100 mmol) was added in portions. The resulting solution was warmed to room temperature and stirred for 18h. The mixture was concentrated in vacuo to give intermediate 1A-a (25.2 g) as a solid.
LCMS (method 9) Rt=0.16 min and 0.25min, m/z 214.1[ M+H ]] +
Step B
(S) -2- ((tert-Butoxycarbonyl) amino) -3- (3-fluoro-4-hydroxyphenyl) -propionic acid methyl ester (intermediate 1B-a)
Saturated sodium bicarbonate solution (200 mL) was added to a vigorously stirred suspension of intermediate 1A-a (25.2 g,100 mmol) in THF (200 mL). The mixture was stirred until gas evolution ceased, then a solution of di-tert-butyl dicarbonate (25.55 g,117 mmol) in THF (20 mL) was added in one portion. The mixture was stirred until significant gas evolution ceased, then stirred for an additional 1.25h. The mixture was partitioned between water (200 mL) and ethyl acetate (200 mL). The organic phase was washed with water (100 mL), and then the combined aqueous phases were washed with ethyl acetate (100 mL). The combined organic phases were washed with brine, dried (sodium sulfate) and concentrated in vacuo to afford intermediate 1B-a (34.2 g).
LCMS (method 9) Rt=0.81 min, m/z 312.1[ M-H ]] -
Step C
4-bromo-3-methyl-1- ((2- (trimethylsilyl) ethoxy) methyl) -1H-pyrrolo [2,3-b]Pyridine compound (intermediate 1C-a)
To a cold (ice/water bath) suspension of sodium hydride (3.41 g 60% dispersion in mineral oil, 85 mmol) in acetonitrile (200 mL) under a stream of nitrogen was added 4-bromo-3-methyl-1H-pyrrolo [2,3-b ] in portions ]Pyridine (20 g,94.8 mmol). The mixture was stirred cold until gas evolution ceased. A solution of 2- (trimethylsilyl) ethoxymethyl chloride (20.1 mL,114 mmol) in acetonitrile (20 mL) was slowly added. The cold mixture was stirred for 2h (maintaining the temperature below 15 ℃) and then diluted with ethyl acetate (200 mL). Water (200 mL) was carefully added. The phases were separated. The organic phase was washed with water (2×100 mL), then brine (100 mL), then dried (Na 2 SO 4 ) And concentrated in vacuo. The residue was chromatographed on a pad of silica gel, eluting with 0-10% ethyl acetate in cyclohexane. The appropriate fractions were concentrated to give intermediate 1C-a (28.3 g).
LCMS (method 7) Rt=1.76 min, m/z 341.1/343.0[ M+H ]] +
Step D
(S) -2- ((tert-Butoxycarbonyl) amino) -3- (3-fluoro-4- ((3-methyl-1- ((2- (trimethylsilyl)) amino) Ethoxy) methyl) -1H-pyrrolo [2,3-b]Pyridin-4-yl) oxy) phenyl) -propionic acid methyl ester (intermediate 1D-a)
Intermediate 1B-a (52.0 g,166 mmol) and1C-a(59.5g,174mmol)、Pd 2 (dba) 3 a mixture of (7.6 g,8.3 mmol), XPhos (7.91 g,17 mmol) and potassium carbonate (49.3 g, 356 mmol) in toluene (600 mL) was purged with argon for 5min. The mixture was heated at 100 ℃ under argon for 5h, then cooled to room temperature, then passed through And (5) filtering. The solvent was evaporated, the residue was diluted with ethyl acetate and the organic layer was washed 3 times with water. The combined aqueous layers were extracted with ethyl acetate and the combined organic extracts were washed with brine, dried (Na 2 SO 4 ) And evaporating. The crude product was chromatographed on a pad of silica gel, eluting with a solution of 10-25% ethyl acetate in isohexane to give the title compound (69.5 g).
LCMS (method 9) Rt=1.88 min, m/z 574.4[ M+H ]] +
Step E
(S) -2- ((tert-Butoxycarbonyl) amino) -3- (3-fluoro-4- ((3-methyl-1- ((2- (trimethylsilyl)) amino) Ethoxy) methyl) -1H-pyrrolo [2,3-b]Pyridin-4-yl) oxy) phenyl) propanoic acid lithium salt (intermediate 1E-a)
Intermediate 1D-a (4.85 g,8.45 mmol) was dissolved in a mixture of methanol (42 mL), water (42 mL) and THF (21 mL). Lithium hydroxide hydrate (1.06 g,25.35 mmol) was added and the reaction mixture was stirred at room temperature for 10min. The solvent was reduced and the product was extracted into ethyl acetate (3×20 mL). The combined organic extracts were washed with brine (30 mL), dried (Na 2 SO 4 ) And evaporated to give the title compound (4.74 g).
LCMS (method 7) Rt=1.79 min, m/z 560.4[ M+H ]] +
Step F
(S) - (1- ((1-Cyclobutylpiperidin-4-yl) amino) -3- (3-fluoro-4- ((3-methyl-1- ((2- (trimethylsilyl)) Alkyl) ethoxy) methyl) -1H-pyrrolo [2,3-b]Pyridin-4-yl) oxy) phenyl) -1-oxopropan-2-yl) aminomethyl Tert-butyl acid (intermediate 1F-a)
To a mixture of intermediate 1E-a (0.2 g,0.37 mmol), 1-cyclobutylpiperidin-4-amine (0.066 g,0.43 mmol) and DIPEA (0.19 mL,1.07 mmol) in DCM (10 mL) was added COMU (0.18 g,0.43 mmol) and the reaction stirred at room temperature for 2h and then concentrated in vacuo. The residue was taken up in ethyl acetate (3X 30 mL) and saturated NaHCO 3 Aqueous solution (20 mL) was partitioned between. The organic layer was washed with brine (20 mL), dried (Na 2 SO 4 ) And evaporated in vacuo to give the desired product, which was used in the next step without further purification.
LCMS (method 9) Rt=1.20 min, m/z 696.5[ M+H ]] +
Step G
(S) -2-amino-N- (1-cyclobutylpiperidin-4-yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2, 3-b)] Pyridin-4-yl) oxy) phenyl) propanamide (example 1)
Intermediate 1F-a (0.28 g,0.403 mmol) was dissolved in a mixture of DCM (10 mL) and TFA (10 mL) and the reaction stirred at room temperature for 1h. The mixture was passed through a 20g scx-2 column eluting with DCM followed by methanol and 2M methanolic ammonia. After 18H of standing, the ammonia solution was evaporated to give a pale yellow residue which was purified by MDAP using an Xb ridge Phenyl column (19X 150mm,10 μm particle size) and 40-100% MeOH/H 2 O(10mM NH 4 CO 3 ) Elution gave the title compound (111 mg).
LCMS (method 1): rt=1.95 min,m/z 466.3[M+H] +
1 H NMR(400MHz,d6-DMSO)δ11.4(s,1H),7.97(d J＝5.45,1H),7.64(d J＝7.94,1H),7.30-7.17(m,2H),7.15-7.04(m,1H),6.15(d J＝5.48Hz,1H),3.51-3.41(m,1H),2.91-2.79(m,1H),2.76-2.55(m,4H),2.38(s,3H),1.99-1.86(m,2H),1.65-1.48(m,4H),1.38-1.15(m,2H)。
Preparation of intermediates 1C-a and 1C-b
The following intermediates were prepared from the indicated starting materials in a similar manner to intermediate 1C-a.
Preparation of intermediate 1D-b
The following intermediates were prepared from the starting materials specified in a similar manner to intermediate 1D-a.
Preparation of intermediate 1E-b
The following intermediates were prepared from the indicated starting materials in a similar manner to intermediate 1E-a.
Intermediate 19C
Step A
4- (Cyclopropylamino) piperidine-1-carboxylic acid benzyl ester (intermediate 19A)
Sodium triacetoxyborohydride (1.36 g, 6)43 mmol) was added in portions to a solution of ice-cooled benzyl 4-oxopiperidine-1-carboxylate (1.0 g,4.29 mmol), cyclopropylamine (0.45 mL,6.43 mmol) and acetic acid (0.37 mL,6.43 mmol) in DCM (10 mL). The resulting mixture was warmed to room temperature and stirred for 18 hours. Additional amounts of cyclopropylamine (0.15 mL,2.15 mmol), acetic acid (0.12 mL,2.15 mmol) and sodium triacetoxyborohydride (0.45 g,2.15 mmol) were added and the resulting mixture was stirred for 7 days. The reaction was quenched by addition of saturated NaHCO 3 The aqueous solution was quenched and extracted with DCM (×3). The organic phase was dried (Na 2 SO 4 ) Filtered and concentrated under reduced pressure. Purification on a 40g Si column eluting with 0-5% 7N methanolic ammonia in DCM gave the desired product (385 mg).
LCMS (method 9) Rt=0.74 min, m/z 275.1[ M+H ]] +
Step B
4- ((tert-Butoxycarbonyl) (cyclopropyl) amino) piperidine-1-carboxylic acid benzyl ester (intermediate 19B)
Intermediate 19A (385 mg,1.40 mmol) was dissolved in THF (4.0 mL), treated with 2M aqueous sodium carbonate (1.2 mL,2.46 mmol) followed by di-tert-butyl dicarbonate (365 mg,1.68 mmol) and the resulting mixture was stirred at room temperature for 72 hours. The mixture was diluted with water and extracted with EtOAc (×3). The organic phase was dried (Na 2 SO 4 ) Filtered and concentrated under reduced pressure. Purification on a 25g Si column eluting with 0-50% EtOAc in cyclohexane afforded the desired product (454 mg).
LCMS (method 9) Rt=1.57 min, m/z 275.1[ M+H-Boc] +
Step C
Cyclopropyl (piperidine-4-Tert-butyl carbamate (intermediate 19C)
Intermediate 19B (454 mg,1.21 mmol) was dissolved in IMS (10.0 mL), treated with 10% palladium on charcoal (50 mg), and the resulting mixture was stirred under hydrogen for 18 hours. Passing the reaction mixture throughFiltered, and the filtrate was concentrated under reduced pressure to give the desired product (264 mg).
LCMS (method 9) Rt=0.74 min, m/z 241.1[ M+H ]] +
Intermediate 20D
Step A
(1-benzyl-4- (hydroxymethyl) piperidin-4-yl) carbamic acid tert-butyl ester (intermediate 20A)
(4-amino-1-benzylpiperidin-4-yl) methanol (500 mg,1.89 mmol) was dissolved in DCM (8.0 mL) and the resulting mixture was cooled with an ice bath. Boc anhydride (470 mg,2.18 mmol) was then added in one portion followed by TEA (0.26 mL) dropwise. The resulting suspension was stirred at room temperature overnight. The reaction was taken up with saturated NaHCO 3 Aqueous (5 mL) wash. The organic phase was dried (Na 2 SO 4 ) Filtered and concentrated under reduced pressure to give a colourless oil. Purification on a Si column eluting with 0-10% DCM in MeOH afforded the desired product (580 mg).
1 H NMR(400MHz,CDCl 3 )d 7.36-7.35(m,5H),5.14-5.12(m,2H),4.52(s,1H),3.79(s,2H),3.70(d,J＝6.1Hz,2H),3.62-3.49(m,1H),3.28-3.20(m,2H),1.89(d,J＝13.4Hz,2H),1.68-1.57(m,2H),1.45(s,9H)。
Step B
(4- (hydroxymethyl) piperidin-4-yl) carbamic acid tert-butyl ester (intermediate 20B)
Intermediate 20A (580 mg,1.59 mmol) and palladium (10%) (169 mg,0.10 mmol) were suspended in IMS (4.0 mL), and the reaction mixture was then exposed to a hydrogen atmosphere via a balloon. The reaction mixture was stirred at room temperature overnight, then the reaction mixture was passed throughThe pad was rinsed and dried to give a pale yellow oil. The residue was used in the next step without purification (367 mg).
1 H NMR(400MHz,CDCl 3 )δ,4.61(s,1H),2.96-2.78(m,4H),
2.73-2.21(m,2H),1.87(d,J＝13.9Hz,2H),1.70-1.59(m,2H),1.44(s,9H)。
Step C
(1-cyclopropyl-4- (hydroxymethyl) piperidin-4-yl) carbamic acid tert-butyl ester (intermediate 20C)
Intermediate 20B (281mg, 1.22 mmol) was dissolved in methanol (5.0 mL), then (1-ethoxycyclopropylpropoxy) trimethylsilane (0.74 mL,3.66 mmol) was added dropwise, and sodium cyanoborohydride (230 mg,3.66 mmol) was added in one portion. The resulting mixture was stirred at 60 ℃ overnight. The reaction mixture was cooled to room temperature and passed through Pad, eluting with methanol. The solution was concentrated, redissolved in ethyl acetate (5 mL) and washed with 1M NaOH (5 mL). The organic phase was dried (Na 2 SO 4 ) Filtered and concentrated to give the desired product (240 mg).
LCMS (method 9) Rt=0.19 min, m/z 271.2[ M+H ]] +
Step D
(4-amino-1-cyclopropylpiperidin-4-yl) methanol (intermediate 20D)
Intermediate 20C (240 mg,0.88 mmol) was dissolved in DCM (4.0 mL) and TFA (2 mL) was added dropwise. The resulting mixture was then stirred at room temperature for 18h. The reaction mixture was loaded onto a 5g SCX-2 column, eluted with methanol and then with 2M methanolic ammonia solution. The eluate was concentrated to give the desired product (151 mg).
LCMS (method 9) Rt=0.17 min, m/z 171.2[ M+H ]] +
Intermediate 21D
Step A
4- ((tert-Butoxycarbonyl) amino) -4-carbamoylpiperidine-1-carboxylic acid benzyl ester (intermediate 21A)
1- ((benzyloxy) carbonyl) -4- ((tert-butoxycarbonyl) amino) piperidine-4-carboxylic acid (500 mg,1.32 mmol) and ammonium chloride (141 mg,2.64 mmol) were stirred in DMF (15 mL) and added(849 mg,1.98 mmol) and DIPEA (0.92 mL,5.29 mmol). The reaction mixture was stirred at room temperature overnight, then the mixture was partitioned between water and ethyl acetate. The phases were separated and the organic phase was then dried (Na 2 SO 4 ) Filtered and concentrated. Purification by flash column chromatography on a 40g Si column eluting with 0-5% DCM in methanol gave the desired product (422 mg).
LCMS (method 9) Rt=1.17 min, m/z 400[ M+Na ]] +
Step B
(4-carbamoyl piperidin-4-yl) carbamic acid tert-butyl ester (intermediate 21B)
Intermediate 21A (420 mg,1.11 mmol) and palladium hydroxide on carbon (20%) (42 mg,0.30 mmol) were suspended in IMS (15 mL), and the reaction mixture was then exposed to a hydrogen atmosphere via a balloon. The reaction mixture was stirred at room temperature for 72h and then passed throughThe pad was rinsed and dried. The white solid was used in the next step without purification (258 mg).
LCMS (method 9) Rt=0.16 min, m/z 244[ M+H ]] +
Step C
(4-carbamoyl-1-cyclopropylpiperidin-4-yl) carbamic acid tert-butyl ester (intermediate 21C)
Intermediate 21B (258 mg,1.06 mmol) was dissolved in methanol (5.0 mL), then (1-ethoxycyclopropylpropoxy) trimethylsilane (0.64 mL,3.18 mmol) was added followed by sodium cyanoborohydride (200 mg,3.18 mmol). The resulting mixture was stirred at 60 ℃ overnight. The reaction mixture was then cooled to room temperature and concentrated. Flash column chromatography on a 25g Si column eluting with 0-5% DCM in methanol afforded the desired product (101 mg).
1 H NMR(400MHz,CDCl 3 )δ6.71(s,1H),5.52(s,1H),4.89(s,1H),3.06-1.65(m,9H),1.46(s,9H),0.52(m,4H)。
Step D
4-amino-1-cyclopropylpiperidine-4-carboxamide (intermediate 21D)
Intermediate 21C (101 mg,0.356 mmol) was dissolved in DCM (4.0 mL) and TFA (2 mL) was added. The resulting mixture was stirred at room temperature for 1h. The reaction mixture was diluted with methanol and then loaded onto a methanol-wet 5g SCX-2 column eluting with methanol and then with 2M methanolic ammonia solution. The ammonia solution was concentrated to give the desired product (68 mg).
LCMS (method 9) Rt=0.24 min, m/z 184.2[ M+H ]] +
Intermediate 31C
Step A
4- (Methylphenylthio) piperidine-1-carboxylic acid tert-butyl ester (intermediate 31A)
To a solution of di-tert-butyl dicarbonate (0.99 mL,4.31 mmol) in DCM (30 mL) was added 4- ((3-methylphenyl) thiopiperidine hydrochloride (1.00 g,4.10 mmol) followed by TEA (1.70 mL,12.31 mmol) the mixture was stirred for 4h while it was warmed to room temperature the reaction mixture was evaporated and then purified by flash column chromatography on 80g Si column eluting with 0-50% EtOAc in cyclohexane to give the title compound (1.14 g).
LCMS (method 9) rt=1.67 min.208.1[ M-Boc+H] +
1 H NMR(300MHz,CDCl 3 )δ7.26(s,1H),7.25-7.23(m,1H),7.22-7.20(m,1H),7.09-7.04(m,1H),3.96(d,J＝12.7Hz,2H),3.25-3.14(m,1H),2.98-2.86(m,2H),2.33(s,3H),1.96-1.85(m,2H),1.60-1.48(m,2H),1.44(s,9H)。
Step B
4- (Methanesulfonyl) piperidine-1-carboxylic acid tert-butyl ester (intermediate 31B)
A solution of intermediate 31A (1.14 g,3.09 mmol) in DCM (30 mL) was cooled to 0deg.C and then 3-chloroperbenzoic acid (1.47 g,8.53 mmol) was added. The mixture was stirred at 0 ℃ for 10 minutes, then it was stirred at room temperature overnight. The reaction mixture was purified by addition of saturated NaHCO 3 Aqueous solution (25 mL) and sodium metabisulfite (916 mg) were quenched and stirred. DCM was added and the organics separated and evaporated using a phase separation column. The crude material was purified by flash column chromatography on an 80g Si column eluting with 0-50% EtOAc in cyclohexane to give the title compound (806 mg).
LCMS (method 9) Rt=1.38, m/z 240.1[ M-Boc+H] +
1 H NMR(300MHz,CDCl 3 )δ7.69-7.63(m,2H),7.48-7.44(m,2H),4.23(d,J＝12.6Hz,2H),3.08-2.96(m,1H),2.72-2.57(m,2H),2.46(s,3H),1.98(d,J＝12.8Hz,2H),1.69-1.53(m,2H),1.43(s,9H)。
Step C
4- (Methanesulfonyl) piperidine (intermediate 31C)
To a solution of intermediate 31B (800 mg,2.36 mmol) in DCM (10 mL) was added TFA (5 mL) under argon and the reaction mixture was stirred at room temperature for 2.75h. The mixture was diluted with methanol and then applied to a methanol-wet SCX-2 column (10 g), washed with methanol and then eluted with 2N ammonia in methanol. The ammonia fraction was evaporated to give the title compound (546 mg).
LCMS (method 9) Rt=0.62 min, m/z 240.1[ M+H ]] +
Examples 2 to 39
In a similar manner to example 1, the following examples were prepared by substituting intermediate 1E and amine starting materials indicated in the following table in step F in the same synthetic sequence.
Example 40
Step A
4-bromo-1-tosyl-1H-pyrrolo [2,3-b]Pyridine (intermediate 40A)
4-bromo-7-azaindole (5.0 g,28.90 mmol) was dissolved in DMF (40 mL) and the solution was stirred at room temperature under a stream of nitrogen. Sodium hydride (60% in mineral oil, 1.50g,37.58 mmol) was added in portions and the reaction stirred for 30min. A solution of 4-toluenesulfonyl chloride (5.77 g,30.37 mmol) in DMF (10 mL) was added dropwise over 10min, and the reaction was stirred for an additional 2h. The reaction mixture was carefully poured into cold water (100 mL) and stirred for 30min. The resulting precipitate was collected by filtration and dried in vacuo to give compound (9.12 g).
LCMS (method 7) Rt=1.59 min, m/z 351.1/353.1[ M+H ]] +
Step B
(S) -2- ((tert-Butoxycarbonyl) amino) -3- (3-fluoro-4- ((1-toluenesulfonyl-1H-pyrrolo [2, 3-b)] Pyridin-4-yl) oxy) phenyl) methyl propionate (intermediate 40B)
Intermediate 1B-a (0.47 g,1.49 mmol), intermediate 40A (0.5 g,1.42 mmol), pd 2 (dba) 3 A solution of (0.065 g,0.071 mmol), XPhos (0.068 g,0.142 mmol), potassium carbonate (0.59 g,4.27 mmol) in toluene (10 mL) was stirred at 95℃for 24h. Passing the reaction mixture throughAnd (5) filtering. The solution diluted with ethyl acetate (50 mL) was washed with water (50 mL). The product was extracted into ethyl acetate (2×50 mL). The combined extracts were dried (Na 2 SO 4 ) And evaporated. The residue was chromatographed on a 25g Si column, eluting with 0-100% ethyl acetate in isohexane to give intermediate 40B (0.273 g).
LCMS (method 9) Rt=1.70 min, m/z 584.3[ M+H ]] +
Step C
(S) -2- ((tert-Butoxycarbonyl) amino) -3- (3-fluoro-4- ((3-iodo-1-tosyl) -1H-pyrrolo [ 2), 3-b]pyridin-4-yl) oxy) phenyl) methyl propionate (intermediate 40C)
NIS (0.11 g,0.49 mmol) was added in portions to the ice-cooled intermediate40B (0.273 g,0.47 mmol) in MeCN (10 mL) and the resulting mixture was warmed to room temperature and stirred for 3h, followed by stirring at 50℃for 2h, then after another portion of NIS (0.33 g,1.47 mmol) had been added, the reaction mixture was stirred at 80℃for an additional 2 days. The reaction was quenched by addition of aqueous sodium metabisulfite (1M) and the resulting mixture was extracted with ethyl acetate (×3). The ethyl acetate layers were separated, combined, dried (Na 2 SO 4 ) And evaporated under reduced pressure. The residue was chromatographed on a column of 10g Si, eluting with 0-100% ethyl acetate in isohexane to give the desired product (0.16 g).
LCMS (method 9) Rt=1.68 min, m/z 710.2[ M+H ]] +
Step D
(S) -2- ((tert-Butoxycarbonyl) amino) -3- (4- ((3-cyclopropyl-1-tosyl-1H-pyrrolo [ 2), 3-b]pyridin-4-yl) oxy) -3-fluorophenyl propionic acid methyl ester (intermediate 40D)
Intermediate 40C (0.50 g,0.71 mmol), cyclopropylboronic acid (0.15 g,1.76 mmol), pd (dppf) Cl 2 .CH 2 Cl 2 A mixture of (0.029 g,0.035 mmol) and potassium carbonate (0.29 g,2.11 mmol) in DMF (5 mL) was sonicated under argon for 5min. The mixture was heated at 100 ℃ for 5h, then cooled to room temperature, and then diluted with water. The mixture was extracted with ethyl acetate, and the organic phase was dried (Na 2 SO 4 ) And evaporating. The crude product was chromatographed on a 40g Si column, eluting with 0-100% ethyl acetate in isohexane to give the desired product (0.17 g).
LCMS (method 9) Rt=1.68 min, m/z 624.3[ M+H ]] +
Step E
(S) -2- ((tert-Butoxycarbonyl) amino) -3- (4- ((3-cyclopropyl-1-tosyl-1H-pyrrolo [ 2), 3-b]pyridin-4-yl) oxy) -3-fluorophenyl propionic acid (intermediate 40E)
Intermediate 40D (0.21 g,0.34 mmol) was dissolved in a mixture of methanol (1.7 mL), water (1.7 mL) and THF (1 mL). Lithium hydroxide hydrate (0.014 g,0.34 mmol) was added and the reaction mixture was stirred at room temperature for 5h. The solvent was reduced and the product was extracted into ethyl acetate (×2). The combined organic extracts were washed with brine, dried (Na 2 SO 4 ) And evaporated to give the title compound (0.15 g).
LCMS (method 9) Rt=1.61 min, m/z 610.1[ M+H ]] +
Step F
(S) - (3- (4- ((3-cyclopropyl-1-tosyl-1H-pyrrolo [2, 3-b)]Pyridin-4-yl) oxy) -3- Fluorophenyl) -1- ((1-methylpiperidin-4-yl) amino) -1-oxopropan-2-yl) carbamic acid tert-butyl ester (intermediate 40F)
Intermediate 40F was prepared from intermediate 40E and 1-methylpiperidin-4-amine using a procedure similar to that used in step F of example 1.
LCMS (method 9) Rt=1.19 min, m/z 706.3[ M+H ]] +
Step G
(S) - (3- (4- ((3-cyclopropyl-1H-pyrrolo [2, 3-b)]Pyridin-4-yl) oxy) -3-fluorophenyl) -1- ((1- Methyl piperidin-4-yl) amino) -1-oxopropan-2-yl carbamic acid tert-butyl ester (intermediate)Body 40G)
Lithium hydroxide monohydrate (20 mg,0.48 mmol) was added to a solution of intermediate 40F (113 mg,0.16 mmol) in a mixture of methanol (0.7 mL), water (0.7 mL) and THF (0.4 mL), and the resulting mixture was stirred at room temperature for 18h and then at 60 ℃ for 2h. The solvent was removed under reduced pressure and the residue was diluted with water and extracted with ethyl acetate (×3). The ethyl acetate layers were separated, combined, dried (Na 2 SO 4 ) And evaporated under reduced pressure to give the desired product (71 mg), which was used in the next step without further purification.
LCMS (method 9) Rt=1.04 min, m/z 552.3[ M+H ]] +
Step H
(S) -2-amino-3- (4- ((3-cyclopropyl-1H-pyrrolo [2, 3-b)]Pyridin-4-yl) oxy) -3-fluorophenyl N- (1-methylpiperidin-4-yl) propanamide (example 40)
Intermediate 40G (71 mg,0.13 mmol) was dissolved in a mixture of DCM (1.0 mL) and TFA (0.2 mL) and the reaction stirred at room temperature for 1h. The mixture was diluted with methanol and passed through a 5g SCX-2 column eluting with methanol and then with 3.5M methanolic ammonia solution. The ammonia solution was evaporated to give a residue which was purified by MDAP using an Xbridge Phenyl column (19X 150mm,10 μm particle size) and 40-60% MeOH/H 2 O(10mM NH 4 CO 3 ) Elution gave the desired compound (13 mg).
LCMS (method 2) Rt=1.91 min, m/z 452.1[ M+H ]] +
1 H NMR(400MHz,DMSO)δ11.42(s,1H),7.98(d,J＝5.4Hz,1H),7.65(d,J＝7.9Hz,1H),7.28-7.20(m,2H),7.10-7.04(m,2H),6.18(d,J＝5.4Hz,1H),3.51-3.46(m,1H),3.37(dd,J＝6.7,6.7Hz,1H),2.86(dd,J＝5.9,13.3Hz,1H),2.74-2.52(m,3H),2.12(s,3H),2.11-2.07(m,1H),1.94-1.88(m,2H),1.66-1.56(m,2H),1.42-1.27(m,2H),0.78(ddd,J＝3.9,6.0,8.3Hz,2H),0.63-0.58(m,2H)。
ee＝36％
Example 41
Step A
(S) - (3, 5-difluoro-4-hydroxyphenyl) -1-oxo-1- (4- (benzenesulfonyl) -piperidin-1-yl) propane-2- Tert-butyl carbamate (intermediate 41A)
A mixture of 4- (benzenesulfonyl) piperidine hydrochloride (1.0 g,3.8 mmol), (S) -2- ((tert-butoxycarbonyl) amino) -3- (3, 5-difluoro-4-hydroxyphenyl) propionic acid (1.1 g,3.5 mmol), DIPEA (1.8 mL,10.4 mmol), DCM (16 mL) and DMF (4 mL) was treated with HATU (1.59 g,4.2 mmol). The mixture was stirred for 1h, and then the resulting solution was allowed to stand for 18h. The mixture was diluted with dichloromethane (30 mL) and washed with saturated sodium bicarbonate (aqueous solution) (20 mL) and then saturated brine (2×20 mL). The organic phase was dried (Na 2 SO 4 ) And concentrated in vacuo and purified by chromatography on a Si column eluting with 0-20% DCM in ethyl acetate to give intermediate 41A (780 mg).
LCMS (method 3) rt=1.25 min. m/z 525.3[ M+H ]] +
Step B
(S) - (3, 5-difluoro-4- ((5-methyl-7- ((2- (trimethylsilyl) ethoxy) -methyl) -7H-pyridine) Pyrrolo [2,3-d]Pyrimidin-4-yl) oxy) phenyl) -1-oxo-1- (4- (benzenesulfonyl) -piperidin-1-yl) propan-2-yl) ammonia Tert-butyl benzoate (intermediate 41B)
Intermediate 41A (315 mg,0.60 mmol), intermediate 1C-b (268 mg,0.9 mmol) and potassium carbonateA mixture of 249mg,1.80 mmol) in DMSO (6 mL) was stirred and heated at 110℃for 2h. The mixture was cooled, diluted with ethyl acetate (30 mL), and washed with a mixture of water (20 mL) and saturated brine (5 mL). The aqueous phase was washed with ethyl acetate (10 mL). The combined organic phases were washed with saturated brine, dried (MgSO 4 ) And concentrated in vacuo. The residue was purified by flash chromatography on a 5g Si column eluting with 0-50% DCM in ethyl acetate to give the desired product (320 mg).
LCMS (method 9) rt=1.81 min. m/z 786.4[ M+H ]] +
Step C
(S) -2-amino-3- (3, 5-difluoro-4- ((5-methyl-7H-pyrrolo [2, 3-d) ]Pyrimidin-4-yl) oxy) benzene Phenyl) -1- (4- (benzenesulfonyl) piperidin-1-yl) propan-1-one (example 41)
Example 41 was prepared from intermediate 41B according to step G of example 1.
LCMS (method 1) 3.13 min, m/z 556.2[ M+H ]] +
1 H NMR (400 mhz, dmso) δ12.01 (s, 1H), 8.22 (apparent d J =10.9 hz, 1H), 7.85 (d J =7.7 hz, 2H), 7.82-7.75 (m, 1H), 7.73-7.65 (m, 2H), 7.27 (s, 1H), 7.16 (d J =9.0 hz, 2H), 4.48 (d J =12.5 hz, 2H), 4.16-4.05 (m, 1H), 3.96-3.88 (m, 1H), 3.63-3.50 (m, 1H), 3.06-2.88 (m, 1H), 2.84-2.76 (m, 1H), 2.70-2.50 (m), 2.43(s) and 2.42(s) (together 3H), 1.91-1.63 (m, 3H), 01.63-1.18 (m, 2H).
Example 42
Step A
(S) - (3- (4- ((6-chloro-1- ((2- (trimethylsilyl) ethoxy) methyl)) -1H-pyrrolo [2,3-b] Pyridin-4-yl) oxy) -3, 5-difluorophenyl) -1-oxo-1- (4- (phenyl-sulfonyl) piperidin-1-yl) propan-2-yl) ammonia Tert-butyl benzoate (intermediate 42A)
A solution of intermediate 1C-C (0.27 g,0.813 mmol), intermediate 41A (0.64 g,1.22 mmol) and potassium carbonate (0.34 g,1.56 mmol) in DMSO (5 mL) was stirred at 120℃for 2h. The reaction mixture was then partitioned between ethyl acetate (3×30 mL) and water (20 mL). The organic layer was washed with brine (20 mL), dried over sodium sulfate and evaporated in vacuo. The residue was chromatographed on a Si column, eluting with 0-70% ethyl acetate in isohexane to give intermediate 42A (460 mg).
LCMS (method 9) Rt=1.80 min, m/z 805.4/807.4[ M+H ]] +
Step B
(S) - (3, 5-difluoro-4- ((1- ((2- (trimethylsilyl) ethoxy) methyl) -1H-pyrrolo [ 2), 3-b]pyridin-4-yl) oxy) phenyl) -1-oxo-1- (4- (benzenesulfonyl) piperidin-1-yl) propan-2-yl) carbamic acid tert-butyl ester Butyl ester (intermediate 42B)
A solution of intermediate 42A (460 mg,0.57 mmol) and trimethylamine (0.096 mL,0.69 mmol) in IMS (20 mL) was stirred at room temperature under a hydrogen atmosphere over 10% palladium on charcoal (50 mg). After 4 days, a second aliquot of 10% palladium on charcoal (50 mg) was added. Stirring was continued for a total of 10 days, and the mixture was then passed throughFiltration and evaporation of the solvent gave the crude product. It was further purified by flash chromatography using a 5g silica column eluting with 0-50% ethyl acetate in DCM to give the desired product (410 mg).
LCMS (method 9) Rt=1.67 min, m/z 771.4[ M ]+H] +
Step C
(S) - (3, 5-difluoro-4- ((3-iodo-1- ((2- (trimethylsilyl) ethoxy) -methyl) -1H-pyrrole And [2,3-b ]]Pyridin-4-yl) oxy) phenyl) -1-oxo-1- (4- (benzenesulfonyl) -piperidin-1-yl) propan-2-yl) amino Tert-butyl formate (intermediate 42C)
NIS (0.13 g,0.578 mmol) was added to a solution of intermediate 42B (0.405 g,0.525 mmol) in MeCN (10 mL) at 0 ℃ and the resulting mixture was stirred at room temperature for 16h. The reaction was quenched by addition of saturated aqueous sodium sulfite (30 mL) and stirred for 30 min. The resulting mixture was extracted with DCM (×2). The DCM layers were washed with brine (20 mL), combined, dried (Na 2 SO 4 ) And evaporated under reduced pressure to give intermediate 42C (0.39 g), which was used in the next step without further purification.
LCMS (method 9) Rt=1.75 min, m/z 897.3[ M+H ]] +
Step D
(S) - (3, 5-difluoro-4- ((3-methyl-1- ((2- (trimethylsilyl) ethoxy) -methyl) -1H-pyridine) Pyrrolo [2,3-b]Pyridin-4-yl) oxy) phenyl) -1-oxo-1- (4- (benzenesulfonyl) -piperidin-1-yl) propan-2-yl) ammonia Tert-butyl benzoate (intermediate 42D)
Intermediate 42C (0.39 g,0.435 mmol), tripotassium phosphate (0.277 g,1.30 mmol) and SPhosPdG2 (0.047 g,0.0652 mmol) were dissolved in THF (9 mL) and purged with nitrogen for 5min, then 2,4, 6-trimethyl-1,3,5,2,4,6-trioxatriborocyclohexane (trioxatrioxariborinane) (0.37 mL,1.30 mmol) and were addedWater (3 mL) and the mixture was purified for an additional 2min, then heated in a microwave reactor at 80℃for 1h. The cold mixture was partitioned between water (20 mL) and ethyl acetate (2 x40 mL). The combined organic phases were washed with water (2×20 mL), HCl (0.5M) (2×20 mL), brine (2×20 mL), dried (Na 2 SO 4 ) And evaporating. The residue was chromatographed on a column of Si, eluting with 0-100% ethyl acetate in isohexane to give the title compound (200 mg).
LCMS (method 9) Rt=1.75 min, m/z 785.4[ M+H ] ] +
Step E
(S) -2-amino-3- (3, 5-difluoro-4- ((3-methyl-1H-pyrrolo [2, 3-b)]Pyridin-4-yl) oxy) benzene Phenyl) -1- (4- (benzenesulfonyl) piperidin-1-yl) propan-1-one (example 42)
Intermediate 42D (0.2 g,0.255 mmol) was dissolved in a mixture of DCM (10 mL) and TFA (10 mL) and the reaction mixture was stirred at room temperature for 1h. The mixture was passed through a 20g scx-2 column eluting with DCM, methanol, and then 2M methanolic ammonia solution. After standing for 18h, the ammonia solution was evaporated to give a pale yellow residue (170 mg). The crude material was purified by MDAP using an Xbridge Phenyl column (19X 150mm,10 μm particle size) and purified with 20-80% MeOH/H 2 O(10mM NH 4 CO 3 ) Elution to give the title compound.
LCMS (method 2) Rt=2.71 min, m/z 555.3[ M+H ]] +
1 H NMR (400 MHz, DMSO). Delta.11.43 (s, 1H), 8.02-7.93 (m, 1H), 7.88-7.82 (m, 2H), 7.81-7.74 (m, 1H), 7.73-7.64 (m, 2H), 7.21 (d J =9.13 Hz, 2H), 7.16 (s, 1H), 6.18-6.09 (m, 1H), 4.55-4.39 (m, 1H), 4.20-4.02 (m, 1H), 3.98-3.84 (m, 1H), 3.67-3.48 (m, 1H), 3.08-2.87 (m, 1H), 2.87-2.74 (m, 1H), 2.72-2.52 (m, 2H), 2.44(s) and 2.42(s) (together 3H), 1.93-1.64 (m, 4H), 1.42-1.42 (m, 2H).
Example 43
Step A
(S) -1- (2- ((tert-Butoxycarbonyl) amino) -3- (3-fluoro-4- ((3-methyl-1- ((2- (trimethylsilyl)) amino) Group) ethoxy) methyl) -1H-pyrrolo [2,3-b]Pyridin-4-yl) oxy) phenyl) -propionylamino) cyclohexane-1-carboxylic acid methyl ester Esters (intermediate 43A)
Intermediate 1E-a (100 mg,0.18 mmol), methyl 1-aminocyclohexane-1-carboxylate (31 mg,0.20 mmol) and COMU (92 mg,0.21 mmol) were dissolved in DCM (3.0 mL) and DIPEA (0.068 mL,0.39 mmol) was added. The reaction was stirred at room temperature for 1.5h. Additional amounts of methyl 1-aminocyclohexane-1-carboxylate (7 mg,0.045 mmol), COMU (19 mg,0.045 mmol) and DIPEA (0.017 ml,0.098 mmol) were added and the resulting mixture was stirred for another 30min. Water was added and the DCM layer was separated. The aqueous layer was further extracted with DCM (×2) and the combined organic extracts were dried (Na 2 SO 4 ) And evaporating. The product was purified by chromatography on a Si column eluting with 0-60% ethyl acetate in cyclohexane to give intermediate 43A (79 mg).
LCMS (method 9) Rt=1.82 min, m/z 699.3[ M+H ]] +
Step B
(S) -1- (2- ((tert-Butoxycarbonyl) amino) -3- (3-fluoro-4- ((3-methyl-1- ((2- (trimethylsilyl)) amino) Group) ethoxy) methyl) -1H-pyrrolo [2,3-b]Pyridin-4-yl) oxy) phenyl) -propionylamino) cyclohexane-1-carboxylic acid (intermediate 43B)
Intermediate 43A (79 mg,0.11 mmol) was dissolved in a mixture of methanol (0.6 mL), water (0.6 mL) and THF (0.3 mL). Adding hydrogen oxide Lithium hydrate (14 mg,0.34 mmol) and the reaction mixture was stirred at room temperature for 2h. An additional amount of lithium hydroxide hydrate (14 mg,0.34 mmol) was added and the reaction mixture was stirred at room temperature for 18h. An additional amount of lithium hydroxide hydrate (14 mg,0.34 mmol) was added and the reaction mixture was stirred at room temperature for 18h. The solvent was reduced and the product was extracted into ethyl acetate (×2). The combined organic extracts were washed with brine, dried (Na 2 SO 4 ) And evaporated to give the desired product (68 mg).
LCMS (method 9) Rt=1.73 min, m/z 685.4[ M+H ]] +
Step C
(S) - (3- (3-fluoro-4- ((3-methyl-1- ((2- (trimethylsilyl) ethoxy) methyl) -1H-pyrrolo) [2,3-b]Pyridin-4-yl) oxy) phenyl) -1- ((1- ((2-methoxyethyl) carbamoyl) -cyclohexyl) amino) -1-oxy Tert-butyl substituted propan-2-yl carbamate (intermediate 43C)
Intermediate 43B (68 mg,0.099 mmol), 2-methoxyethylamine (8.2 mg,0.11 mmol) and COMU (51 mg,0.12 mmol) were dissolved in DCM (1.7 mL) and DIPEA (0.038 mL,0.22 mmol) was added. The reaction was stirred at room temperature for 3.5h. Additional amounts of 2-methoxyethylamine (8.2 mg,0.11 mmol), COMU (51 mg,0.12 mmol) and DIPEA (0.038 ml,0.22 mmol) were added and the resulting mixture was stirred for an additional 2h. Water was added and the DCM layer was separated. The aqueous layer was further extracted with DCM (×2) and the combined organic extracts were dried (Na 2 SO 4 ) And evaporating. The product was purified by chromatography on a Si column eluting with 0-100% ethyl acetate in cyclohexane to give intermediate 43C (28 mg).
LCMS (method 9) Rt=1.72 min, m/z 742.4[ M+H ]] +
Step D
(S) -1- (2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2, 3-b)]Pyridin-4-yl) oxy) phenyl) Propionylamino) -N- (2-methoxyethyl) cyclohexane carboxamide (example 43)
Example 43 was prepared from intermediate 43C using a method similar to that used in step G of example 1.
LCMS:Rt＝2.59min,m/z 512.3[M+H] + (method 1)
1 H NMR(400MHz,DMSO)δ11.40(d,J＝1.3Hz,1H),7.98(d,J＝5.4Hz,1H),7.69(s,1H),7.40(dd,J＝5.7,5.7Hz,1H),7.33(dd,J＝1.9,12.0Hz,1H),7.24(dd,J＝8.4,8.4Hz,1H),7.16-7.13(m,2H),6.17(d,J＝5.4Hz,1H),3.53(dd,J＝5.7,7.8Hz,1H),3.29-3.25(m,2H),3.22(s,3H),3.21-3.14(m,2H),2.96(dd,J＝5.6,13.5Hz,1H),2.73-2.66(m,1H),2.38(d,J＝1.0Hz,3H),2.03-1.89(m,3H),1.66-1.56(m,2H),1.48-1.45(m,3H),1.33(d,J＝12.2Hz,1H),1.19-1.14(m,1H)。
Example 44
Step A
(S) - (3- (3-fluoro-4- ((3-methyl-1- ((2- (trimethylsilyl) ethoxy) methyl) -1H-pyrrolo) [2,3-b]Pyridin-4-yl) oxy) phenyl) -1- ((1- ((2-hydroxyethyl) carbamoyl) -cyclohexyl) amino) -1-oxo Tert-butyl propan-2-yl carbamate (intermediate 44A)
Intermediate 43B (138 mg,0.20 mmol) was dissolved in DMF (1.0 mL) and cooled in an ice-bath. TBTU (129 mg,0.40 mmol), HOBt (54 mg,0.40 mmol) and DIPEA (0.11 mL,0.60 mmol) were added and the resulting mixture was stirred for 10 min. Ethanolamine (0.015 mL,0.24 mmol) was added and the mixture warmed to room temperature and stirred for 5h. Adding water and ethyl acetate, and mixingThe layers were separated. The aqueous layer was further extracted with ethyl acetate (×2) and the combined organic extracts were dried (Na 2 SO 4 ) And evaporating. The product was purified by chromatography on a 25gSi column eluting with 0-100% ethyl acetate in cyclohexane to give intermediate 44A (68 mg).
LCMS(U1152340):Rt＝1.63min,m/z 728.4[M+H] +
ee％＝73％
Step B
(S) -1- (2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2, 3-b)]Pyridin-4-yl) oxy) phenyl) Propionylamino) -N- (2-hydroxyethyl) cyclohexane carboxamide (example 44)
Example 44 was prepared from intermediate 44A using the conditions described in step G of example 1.
LCMS (method 1) Rt=2.47 min, m/z 498.3[ M+H ]] +
1 H NMR(400MHz,DMSO)δ11.39(s,1H),7.98(d,J＝5.4Hz,1H),7.73(s,1H),7.38-7.31(m,2H),7.24(dd,J＝8.4,8.4Hz,1H),7.15-7.12(m,2H),6.18-6.16(m,1H),4.52(s,1H),3.54(dd,J＝5.9,7.8Hz,1H),3.16-3.03(m,2H),2.95(dd,J＝5.8,13.5Hz,1H),2.74-2.66(m,1H),2.38(d,J＝1.0Hz,3H),2.09-1.83(m,3H),1.67-1.56(m,2H),1.48-1.45(m,3H),1.36-1.31(m,1H),1.15(dd,J＝9.2,9.2Hz,2H)。
Examples 45 to 47 were prepared
In a similar manner to example 1, the following examples were prepared according to the same synthesis sequence by substituting the amine starting materials indicated in the following table in step F and using 5 equivalents of DIPEA.
Examples 8a and 8b preparation
The compound (2S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N- (1, 3-trimethylpiperidin-4-yl) propionamide of example 8 was resolved using the conditions given in the following table to give two separate single diastereomers:
the first eluting single diastereomer (first diastereomer) of (2S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N- (1, 3-trimethylpiperidin-4-yl) acrylamide (example 8 a)
The second eluting single diastereomer (second diastereomer) of (2S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N- (1, 3-trimethylpiperidin-4-yl) acrylamide (example 8 b)
Pharmacological Activity of Compounds of the invention
In vitro inhibition Activity assay description
Using ADP-Glo kit (Promega) 10. Mu.l containing 40mM Tris pH7.5, 20mM MgCl 2 The effectiveness of the compounds of the invention in inhibiting Rho kinase activity was determined in an assay of 0.1mg/ml BSA, 50. Mu.M DTT and 2.5. Mu.M peptide substrate (myelin basic protein). Compounds were dissolved in DMSO such that the final concentration of DMSO in the assay was 1%. All reactions/incubations were performed at 25 ℃. The compound (2. Mu.l) and Rho kinase 1 or 2 (4. Mu.l) were mixed and incubated for 30 minutes. The reaction was started by adding ATP (4. Mu.l) such that the final concentration of ATP in the assay was 10. Mu.M. After 1 hour incubation, 10. Mu.l ADP-Glo reagent was added and after an additional 45 minutes incubation, 20. Mu.l kinase assay buffer was added and the mixture was incubated for an additional 30 minutes. The luminescence signal is measured on a photometer. The control consisted of assay wells without compound and the background was determined using assay wells without enzyme added. Test compounds in a dose-response format and calculate kinase at each concentration of the compound Inhibition of activity. To determine IC 50 (concentration of compound required to inhibit 50% of enzyme activity) data were fitted to% inhibition versus Log using sigmoid fit with variable slope 10 Plot of compound concentration, and fix maximum to 100% and minimum to 0%. To determine Ki values, the Cheng-Prusoff equation (ki=ic 50 /(1+[S]/Km)。
The compounds according to the invention exhibit Ki values below 5 μm and Ki is even below 500nM for most of the compounds of the invention.
The results for the various compounds of the examples are provided in table 1 below and expressed as ranges of activity.
TABLE 1
Wherein the compounds are classified in a manner with respect to their potency for the inhibitory activity of ROCK-I and ROCK-II isoforms according to the following classification criteria:
+++：Ki<3nM
++: ki is in the range of 3-30nM
+：Ki>30nM

Claims (6)

1. A compound selected from:
(S) -2-amino-N- (1-cyclobutylpiperidin-4-yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propanamide;
(2S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N- (1- (tetrahydrofuran-3-yl) piperidin-4-yl) propionamide;
(S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N- (1- (oxetan-3-yl) piperidin-4-yl) propionamide;
(S) -2-amino-N- (1, 4-dimethylpiperidin-4-yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propanamide;
(S) -2-amino-N- (1-cyclopropylpiperidin-4-yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propanamide;
(S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N- (1-isobutylpiperidin-4-yl) propionamide;
(S) -2-amino-N- (1-ethylpiperidin-4-yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propanamide;
(2S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N- (1, 3-trimethylpiperidin-4-yl) propionamide;
(S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -1- (4-hydroxy-4- (hydroxymethyl) piperidin-1-yl) propan-1-one;
(S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N- ((R) -1-methylpyrrolidin-3-yl) propionamide;
(S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -1- (7-methyl-4, 7-diazaspiro [2.5] octan-4-yl) propan-1-one;
(S) -2-amino-1- ((3 ar,6 as) -5-cyclopropyl-hexahydropyrrolo [3,4-c ] pyrrol-2 (1H) -yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propan-1-one;
(S) -2-amino-1- ((1S, 4S) -5-cyclopropyl-2, 5-diazabicyclo [2.2.1] heptan-2-yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propan-1-one;
(S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N- (1- (2-methoxyethyl) piperidin-4-yl) propionamide;
(S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N- (1- (3-fluoropropyl) piperidin-4-yl) propionamide;
(S) -2-amino-N- (1- (cyclopropylmethyl) piperidin-4-yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propanamide;
(2S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N- (1-methylpiperidin-3-yl) propionamide;
(S) -2-amino-1- (4- (cyclopropylamino) piperidin-1-yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propan-1-one;
(S) -2-amino-N- (1-cyclopropyl-4- (hydroxymethyl) piperidin-4-yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propanamide;
(S) -4- (2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propionylamino) -1-cyclopropylpiperidine-4-carboxamide;
(S) -2-amino-1- (3, 4-dihydro-2, 7-naphthyridin-2 (1H) -yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propan-1-one;
(S) -2-amino-1- (5, 8-dihydropyrido [3,4-d ] pyrimidin-7 (6H) -yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propan-1-one;
(S) -2-amino-1- (6, 7-dihydrothiazolo [5,4-c ] pyridin-5 (4H) -yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propan-1-one;
(S) -2-amino-1- (3, 4-dihydro-2, 6-naphthyridin-2 (1H) -yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propan-1-one;
(S) -2-amino-1- (7, 8-dihydro-1, 6-naphthyridin-6 (5H) -yl) -3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propan-1-one;
(S) -1- (2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propionylamino) cyclobutane-1-carboxamide;
(S) -1- (2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propionylamino) -N-methylcyclopentane-1-carboxamide;
(S) -1- (2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propionylamino) -N-methylcyclohexane-1-carboxamide;
(S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N- (1- (hydroxymethyl) cyclobutyl) propionamide;
(S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -1- (4- (m-toluenesulfonyl) piperidin-1-yl) propan-1-one;
(S) -1- (2-amino-3- (3-fluoro-4- ((5-methyl-7H-pyrrolo [2,3-d ] pyrimidin-4-yl) oxy) phenyl) propionylamino) cyclohexanecarboxamide;
(S) -1- (2-amino-3- (3-fluoro-4- ((5-methyl-7H-pyrrolo [2,3-d ] pyrimidin-4-yl) oxy) phenyl) propionylamino) -N-methylcyclohexane carboxamide;
(S) -2-amino-3- (3-fluoro-4- ((5-methyl-7H-pyrrolo [2,3-d ] pyrimidin-4-yl) oxy) phenyl) -N- (1- (2-hydroxyethyl) cyclohexyl) propionamide;
(S) -2-amino-3- (3-fluoro-4- ((5-methyl-7H-pyrrolo [2,3-d ] pyrimidin-4-yl) oxy) phenyl) -N- (1- (hydroxymethyl) cyclohexyl) propionamide;
(S) -2-amino-3- (3-fluoro-4- ((5-methyl-7H-pyrrolo [2,3-d ] pyrimidin-4-yl) oxy) phenyl) -N- (4- (hydroxymethyl) tetrahydro-2H-pyran-4-yl) propionamide;
(S) -2-amino-3- (3-fluoro-4- ((5-methyl-7H-pyrrolo [2,3-d ] pyrimidin-4-yl) oxy) phenyl) -1- (4- ((4-fluorophenyl) sulfonyl) piperidin-1-yl) propan-1-one;
(S) -2-amino-3- (3-fluoro-4- ((5-methyl-7H-pyrrolo [2,3-d ] pyrimidin-4-yl) oxy) phenyl) -1- ((S) -3- (benzenesulfonyl) pyrrolidin-1-yl) propan-1-one;
(S) -2-amino-N- (1-cyclopropylpiperidin-4-yl) -3- (3-fluoro-4- ((5-methyl-7H-pyrrolo [2,3-d ] pyrimidin-4-yl) oxy) phenyl) propanamide;
(S) -2-amino-3- (4- ((3-cyclopropyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) -3-fluorophenyl) -N- (1-methylpiperidin-4-yl) propionamide;
(S) -2-amino-3- (3, 5-difluoro-4- ((5-methyl-7H-pyrrolo [2,3-d ] pyrimidin-4-yl) oxy) phenyl) -1- (4- (benzenesulfonyl) piperidin-1-yl) propan-1-one;
(S) -2-amino-3- (3, 5-difluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -1- (4- (benzenesulfonyl) piperidin-1-yl) propan-1-one;
(S) -1- (2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propionylamino) -N- (2-methoxyethyl) cyclohexane carboxamide;
(S) -1- (2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propionylamino) -N- (2-hydroxyethyl) cyclohexane carboxamide;
(S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N, N-dimethylpropionamide;
(S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N-methylpropanamide;
(S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) propanamide;
(2S) -2-amino-3- (3-fluoro-4- ((3-methyl-1H-pyrrolo [2,3-b ] pyridin-4-yl) oxy) phenyl) -N- (1, 3-trimethylpiperidin-4-yl) propionamide;
or a pharmaceutically acceptable salt thereof.
2. A pharmaceutical composition comprising a compound as defined in claim 1, or a pharmaceutically acceptable salt thereof, in admixture with one or more pharmaceutically acceptable carriers or excipients.
3. The pharmaceutical composition according to claim 2, which is suitable for administration by inhalation, selected from the group consisting of inhalable powders, propellant-containing metered dose aerosols or propellant-free inhalable formulations.
4. Use of a compound according to claim 1 in the manufacture of a medicament for the prevention and/or treatment of a pulmonary disease selected from: asthma, chronic obstructive pulmonary disease, idiopathic pulmonary fibrosis, and pulmonary hypertension.
5. Use of a compound according to claim 1 in the manufacture of a medicament for the prevention and/or treatment of a pulmonary disease, which is pulmonary arterial hypertension.
6. A combination of a compound as defined in claim 1 with one or more active ingredients selected from the following classes: organic nitrates and NO donors; NO inhaled; a stimulus of a soluble guanylate cyclase; agonists of the prostacyclin analog PGI2 and prostacyclin receptor; a compound that inhibits degradation of cyclic guanylic acid and/or cyclic adenylate; human neutrophil elastase inhibitors; compounds that inhibit the signal transduction cascade; an active substance for lowering blood pressure; neutral endopeptidase inhibitors; a penetrant; ENaC blockers; anti-inflammatory agents, including corticosteroids and antagonists of chemokine receptors; antihistamines; antitussive; antibiotics and dnase medicinal substances and selective cleavage agents; agents that inhibit ALK5 and/or ALK4 phosphorylation of Smad2 and Smad 3; tryptophan hydrolase 1 inhibitors and multi-kinase inhibitors.
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