CN112730398A

CN112730398A - Preparation method, use method and detection device of rectal part detection reagent

Info

Publication number: CN112730398A
Application number: CN202011564728.9A
Authority: CN
Inventors: 林丽萍
Original assignee: Guangdong Lvkang Medical Equipment Co ltd
Current assignee: Guangdong Lvkang Medical Equipment Co ltd
Priority date: 2020-12-25
Filing date: 2020-12-25
Publication date: 2021-04-30

Abstract

Discloses a preparation method, a using method and a detection device of a detection reagent for rectum parts, which have the advantages of simple operation, low cost, short detection period, no need of going to hospitals and avoidance of cross infection. The detection reagent for the rectal part comprises a reagent I, a reagent II and a reagent III; each part of reagent I consists of 0.2g of tetramethylbenzidine, 3ml of dimethyl sulfoxide, 1.6g of citric acid, 0.6g of sodium ethylene diamine tetracetate, 2ml of glacial acetic acid and 995ml of purified water; each part of reagent II consists of 200ml of hydrogen peroxide, 2.4g of sodium acetate, 10g of citric acid and 800ml of purified water; each reagent III consists of 10g of sodium chloride, 2g of citric acid and 1000ml of purified water.

Description

Preparation method, use method and detection device of rectal part detection reagent

Technical Field

The invention belongs to the technical field of medical instruments, and particularly relates to a preparation method of a detection reagent for a rectal part and a detection device using the detection reagent.

Background

The colorectal cancer is the third most advanced cancer next to lung cancer and gastric cancer in China, and according to the statistical data of 'expert consensus of screening strategy for early diagnosis of colorectal tumor in China' in 2018, 42.92 thousands of new cases and 28.14 thousands of death cases are shown every year, so that the prevention and control situation is severe. However, there is a long process for colorectal cancer to progress from precancerous lesions (adenomas) to malignant lesions, and colorectal cancer is one of the few malignant tumors that can be detected and treated early by screening. Data from screening cost analysis of large intestine cancer of Beijing population shows that the first stage of colorectal cancer detected by the examinee through physical examination accounts for 72%, while the first stage of colorectal cancer detected in outpatient clinic accounts for 13%, and 28% of patients are already detected in advanced stage. Therefore, the compliance rate of enteroscope screening can be improved through physical examination screening, and early colorectal cancer can be effectively discovered, so that early discovery, early diagnosis and early treatment of intestinal diseases are realized.

The existing rectal site detection needs instruments, but the instruments used in hospitals are easy to cause cross infection due to the fact that disinfection is not in place, and frost is added to the snow of patients.

Ferroprotoporphyrin, which is widely present in cells, is involved in the constitution of various enzymes, and normally functions as a binding protein in a normal physiological state. When pathogenic factors cause cell stability disorder, stable structures in rectal mucosa cells are destroyed, metabolism disorder and intracellular protein conformation change, certain lipophilic substances enter hydrophobic cores containing ferrous protoporphyrin protein, so that the ferrous protoporphyrin falls off into a free state, and free ferrous protoporphyrin in the rectal mucosa cells is increased. The ferrous ion is used as a third major metabolite of heme catalyzed by heme oxygenase, is not only a nutrient substance and promotes the growth and proliferation of cells, but also can generate excessive ROS through Fenton reaction due to the multi-position endowment of the Fe-O-H. Meanwhile, the regulation of the tumor cells on iron also provides convenience for self growth, and promotes the self growth and drug resistance. Recent studies have found that iron-regulated proteins can promote extrinsic modification of DNA and histone regulatory proteins, such as Jumonj idemain-accompanying protein 2A (JMJD2A), and that this abnormal change can be reversed by iron chelators; it has been reported that the synthesis of ribonuclease and deoxyribonuclease depends on the participation of iron during cell division, and iron deficiency finally leads to the termination of cell division activity by influencing the activity of ribonuclease. In addition, iron metabolism levels regulate other proteins associated with cell cycle, DNA damage, such as Mdm2, GADD45, p21, and WAF 1.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: overcomes the defects of the prior art, provides a preparation method of a rectal detection reagent, has simple operation, short detection period and low price, does not need to go to a hospital, and avoids cross infection.

The technical solution of the invention is as follows: the preparation method of the rectal detection reagent comprises the steps of preparing a rectal detection reagent, wherein the rectal detection reagent comprises a reagent I, a reagent II and a reagent III;

each part of reagent I consists of 0.2g of tetramethylbenzidine, 3ml of dimethyl sulfoxide, 1.6g of citric acid, 0.6g of sodium ethylene diamine tetracetate, 2ml of glacial acetic acid and 995ml of purified water;

each part of reagent II consists of 200ml of hydrogen peroxide, 2.4g of sodium acetate, 10g of citric acid and 800ml of purified water;

each reagent III consists of 10g of sodium chloride, 2g of citric acid and 1000ml of purified water.

The present invention stains cells containing a target by a redox reaction of a reagent I and a reagent II with a reagent III containing the target. Therefore, the free heme in the rectal mucosal cell can be detected, the detection reagent can be operated by an individual, the operation is simple, the detection period is short, the price is low, the hospital does not need to be visited, and the cross infection is avoided.

Also provided is a method of using the rectal site detection reagent, the method comprising the steps of:

(1) sampling;

(2) processing a sample;

(3) taking a reagent I, and pouring the reagent I into a test pool on a box support;

(4) sucking 1-2ml of the completely dissolved and mixed sample treatment liquid by using a plastic suction pipe and dripping the sample treatment liquid into a test pool;

(5) taking a reagent II, and pouring the reagent II into a test pool on the box support;

(6) comparing the color development in the test cell with a colorimetric plate within 180 seconds to obtain a result;

(7) and (5) judging a result: the color is negative when the color is not changed, and the color is changed into light blue, green and blue-green and is positive.

There is also provided a device for detecting a rectal site, comprising: reagent I, reagent II, reagent III, plastics straw, box hold in the palm, sample thief, colour comparison card, packing carton, sample part.

Drawings

FIG. 1 shows a flow chart of a method for using a rectal site detection reagent according to the present invention.

Detailed Description

The preparation method of the rectal detection reagent comprises the steps of preparing a rectal detection reagent, wherein the rectal detection reagent comprises a reagent I, a reagent II and a reagent III;

As shown in figure 1, a method for using the rectal site detection reagent is also provided, the method comprising the steps of:

(1) sampling;

(2) processing a sample;

Preferably, in the step (1), the sampling site is not cleaned, medicine is applied, scratching is carried out, the anoscope is placed into the dentate line of the anal canal through the anus, and rectal mucus sampler is used for wiping above the dentate line to obtain the rectal mucosa exudate.

Preferably, in the step (2), the head of the sampler is placed in the sample preservation solution and stirred, so that the sampled specimen and the sample preservation solution are uniformly mixed and are kept stand.

There is also provided a device for detecting a rectal site, comprising: reagent I, reagent II, reagent III, plastics straw, box hold in the palm, sample thief, colour comparison card, packing carton, sample part. The product has the advantages of noninvasive sampling, low cost, simple and convenient operation and wide applicable population, reduces the uncomfortable feeling of a user and has high acceptance degree of the user.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and all simple modifications, equivalent variations and modifications made to the above embodiment according to the technical spirit of the present invention still belong to the protection scope of the technical solution of the present invention.

Claims

1. The preparation method of the rectal detection reagent is characterized by comprising the following steps: the detection reagent for the rectal part comprises a reagent I, a reagent II and a reagent III;

2. The application method of the detection reagent for the rectal area is characterized in that: the method comprises the following steps:

(1) sampling;

(2) processing a sample;

3. The method for using a rectal site test reagent according to claim 2, characterized in that: in the step (1), the sampling part is not cleaned, medicine is applied, scratching is carried out, the anoscope is placed into the dentate line of the anal canal through the anus, and rectal mucus sampler is used for wiping above the dentate line to obtain the rectal mucosa exudate.

4. The method for using a rectal site test reagent according to claim 3, characterized in that: and (2) putting the head of the sampler into the sample preservation solution and stirring to uniformly mix the sampled specimen with the sample preservation solution, and standing.

5. Rectal area's detection device which characterized in that: it includes: reagent I, reagent II, reagent III, plastics straw, box hold in the palm, sample thief, colour comparison card, packing carton, sample part.