CN112577902A - Centrifugal micro-fluidic chip for glycated albumin detection and use method thereof - Google Patents
Centrifugal micro-fluidic chip for glycated albumin detection and use method thereof Download PDFInfo
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Abstract
The invention provides a centrifugal microfluidic chip for glycated albumin detection and a using method thereof, wherein the centrifugal microfluidic chip comprises a chip disc, a sample conveying unit, a diluent conveying unit, a mixing tank and a detection unit are arranged on the surface of the chip disc, the detection unit is arranged at the edge of the chip disc, the mixing tank is arranged between the diluent conveying unit and the detection unit, the diluent quantifying tank of the diluent conveying unit is connected to the mixing tank through a first siphon flow channel, the sample quantifying tank of the sample conveying unit is connected to the mixing tank through a second siphon flow channel, and the mixing tank is connected to the detection unit through a third siphon flow channel. The method can integrate the operation processes of sample separation, quantification, dilution and mixing, sample conveying, reaction detection and the like, greatly reduce the dosage of the detection sample and the dosage of the detection reagent by the accurate operation of trace fluid, miniaturize, integrate and automate the detection platform, and solve the problem of interference on the detection of the glycated albumin caused by the intrinsic glycated amino acid contained in the detection sample.
Description
Technical Field
The invention belongs to the field of glycated albumin detection, and particularly relates to a centrifugal microfluidic chip for glycated albumin detection.
Background
Diabetes is a lifelong disease that has become a major health care and hygiene problem in countries around the world due to its high complications and mortality. Clinically, diabetes is mainly determined by detecting blood glucose parameters, but the blood glucose parameters only represent blood glucose levels during blood drawing and have limitations on accurate diagnosis. At present, glycated hemoglobin is the gold standard for measuring glycemic control and is also an important means for diagnosing and managing diabetes. However, recent medical studies have shown that: glycated albumin is an index that reflects the average blood glucose level over the past 2-3 weeks. The reaction period is shorter than that of the 'gold standard' glycosylated hemoglobin in the blood glucose detection. Therefore, Glycated Albumin (GA) is advantageous over glycated hemoglobin in confirming therapeutic effects and adjusting clinical drug doses. In addition, in many cases of abnormal hemoglobin metabolism, the result of glycated hemoglobin is affected and cannot truly reflect the blood glucose level of the patient, while the result of Glycated Albumin (GA) is not affected, such as the blood glucose test of diabetic nephropathy dialysis patients, anemia patients, pregnant women, etc., so Glycated Albumin (GA) is the first choice for blood glucose monitoring. At present, the detection of the glycated albumin is mainly completed by a large-scale biochemical analyzer, and the methods bring the defects of large sample consumption, complex operation procedure, high instrument cost and the like, and often require the operation environment at the laboratory level and professional technical operators, so that the popularization and the application of the timely and rapid detection of the diabetes are seriously hindered by the defects. Therefore, the development of a rapid, portable, intuitive, and easy-to-operate miniature detection system for glycated albumin has become a key point of domestic and foreign research.
The micro-fluidic chip technology is a new technology for accurately controlling and controlling nano-liter and pico-liter fluid (biological sample fluid) in a flow channel with a micron scale, and can integrate basic operation units of sample preparation, reaction, separation, detection, cell culture, sorting, cracking and the like in the fields of chemistry, biology and the like or a chip with a few square centimeters (even smaller) by applying the technology, and a network is formed by a micro-flow channel so as to control the fluid to penetrate through the whole system and replace a technical platform with various functions of a conventional chemical or biological laboratory. The basic characteristics and the greatest advantages of the microfluidic chip laboratory are that various unit technologies are flexibly combined and integrated on a small platform with controllable whole;
because the detection sample (serum or plasma) generally contains endogenous glycated amino acids, which can interfere the detection of glycated albumin, the traditional detection method of glycated albumin adopts a double-reagent detection method, firstly, the endogenous glycated amino acids in the detection sample are converted into glucose ketoaldehyde, amino acids and hydrogen peroxide by ketoamine oxidase, and then, the interfering substances in the detection sample are removed by the treatment solution. If the scheme is adopted in the microfluidic chip, the structural design of the chip is complex, the reliability in the detection process is reduced, and the detection result is inaccurate due to the fact that the hydrogen peroxide generated by the reaction of the endogenous glycated amino acid is not completely removed and the subsequent reaction is interfered. In order to solve the problem, the invention provides a micro-fluidic chip for detecting glycated albumin and a using method thereof, which can effectively solve the problem that the detection of glycated albumin is interfered by endogenous glycated amino acids contained in a detection sample.
Disclosure of Invention
In view of the above, the present invention is directed to a centrifugal microfluidic chip for glycated albumin detection and a method for using the same, which can automatically implement the whole processes of plasma separation, quantification, mixing, reaction, detection, etc. in the chip only by adding a detection sample, and the operation mode greatly simplifies the operation process, facilitates the integration, miniaturization, and automation of the detection platform, and implements the detection in the microfluidic chip, thereby greatly reducing the usage of the detection sample and the detection reagent, and reducing the detection time; and the problem that the detection of the glycated albumin is interfered by the intrinsic glycated amino acid contained in the detection sample can be effectively solved.
In order to achieve the purpose, the technical scheme of the invention is realized as follows:
the utility model provides a centrifugal micro-fluidic chip for glycated albumin detects, including the chip dish, chip dish surface is equipped with sample transport unit, diluent transport unit, mixing tank and detecting element, diluent transport unit sets up the first side at the chip dish, sample transport unit sets up the second side at the chip dish, detecting element sets up the edge at the chip dish, the mixing tank sets up between diluent transport unit and detecting element, diluent transport unit's diluent ration groove is connected to the mixing tank through a siphon runner, sample transport unit's sample ration groove is connected to the mixing tank through No. two siphon runners, the mixing tank is connected to detecting element through No. three siphon runners.
Further, the whole fan-shaped structure that is of chip dish, diluent conveying unit include diluent holding tank, diluent cross the aqueduct, diluent ration groove, and the chip dish sets gradually diluent holding tank, diluent cross the aqueduct, diluent ration groove from the direction of inner arc outer arc, and the diluent holding tank passes the aqueduct through a microchannel intercommunication to the diluent and crosses the aqueduct, and the diluent passes the aqueduct through No. two microchannel intercommunication to the diluent ration groove.
Further, the sample conveying unit comprises a sample loading slot and a sample quantifying slot, the chip tray is sequentially provided with the sample loading slot and the sample quantifying slot from the direction of the inner arc to the outer arc, and the sample loading slot is connected to the sample quantifying slot through a third micro-channel.
Further, the detecting unit includes a plurality of inspection holes and a plurality of locating hole, and inspection hole and locating hole agree with the even distribution of outer arc of chip dish on the chip dish, and the quantity of inspection hole is 7, is equipped with a locating hole between per two inspection holes, and wherein 4 inspection holes that are close to the mixing tank communicate to the mixing tank through the runner, and 4 inspection holes are from a left side to the right side as inspection hole, inspection hole No. two, inspection hole No. three and inspection hole No. four in proper order.
Furthermore, a first freeze-drying reagent is arranged in the first detection hole, the first freeze-drying reagent is a detection reagent for endogenous glycated amino acids, the main components of the first freeze-drying reagent are ketoamine oxidase or fructosyl oxidase, catalase and a color source material, and auxiliary materials, excipients and protective agents are also arranged.
Furthermore, a second freeze-drying reagent is arranged in the second detection hole, the second freeze-drying reagent is a detection reagent of glycated albumin, and the main components of the second freeze-drying reagent are proteolytic enzyme, ketoamine oxidase, catalase, a color source material and fructosyl amino acid oxidase, and auxiliary materials, excipients and protective agents are also arranged.
Furthermore, a third freeze-drying reagent is arranged in the third detection hole, the third freeze-drying reagent is an albumin detection reagent, the main component of the third freeze-drying reagent is bromocresol green, and auxiliary materials, excipients and protective agents are also arranged.
Furthermore, the excipient is inert substances, including sucrose, glucose, trehalose, melezitose, dextran and mannitol, and polymer PEG and PVP; the protective agent is protein, including BSA, gelatin, and collagen.
Furthermore, the width of the first micro-channel, the second micro-channel and the third micro-channel is 0.01-1mm, and the depth is 0.01-1 mm; the inner surfaces of the first siphon runner and the second siphon runner are hydrophilic surfaces.
A method for using a centrifugal microfluidic chip for glycated albumin assay,
s1, placing the packaged diluent sac into a diluent storage tank, adding a detection sample into a sample adding slot, and then placing the chip tray on a detection platform of a biochemical immune machine for fixation;
s2, controlling the biochemical immune machine to start, opening the diluent bag, rotating the motor of the biochemical immune machine, enabling the diluent to enter the diluent quantifying groove to complete quantification, and enabling the sample to enter the sample quantifying groove to complete quantification of the sample; the time for the motor to rotate is 10-300 s;
s3, stopping the rotation of the motor, and opening the first siphon flow passage and the second siphon flow passage due to the hydrophilicity of the first siphon flow passage and the second siphon flow passage; the motor is started, the diluent in the diluent quantifying groove enters the mixing groove through the first siphon flow channel under the action of centrifugal force generated by the rotation of the motor, and the sample in the sample quantifying groove enters the mixing groove through the second siphon flow channel under the action of centrifugal force generated by the rotation of the motor;
s4, the sample and the diluent enter a mixing tank, and the motor is controlled to rotate at the acceleration and deceleration moment to generate a vibration effect so that the sample and the diluent are fully mixed;
s5, stopping rotation of the motor, opening the third siphon flow channel, enabling a mixed solution of the sample and the diluent to sequentially enter the first detection hole, the second detection hole, the third detection hole and the fourth detection hole through the third siphon flow channel under the action of centrifugal force, starting the motor, and performing acceleration and deceleration rotation to enable the mixed solution to respectively react with reagents in the first detection hole, the second detection hole, the third detection hole and the fourth detection hole;
s6, after the reaction is finished, the optical module of the detection platform detects the first detection hole, the second detection hole, the third detection hole and the fourth detection hole, the photoelectric conversion module converts the optical signals in the first detection hole, the second detection hole, the third detection hole and the fourth detection hole into mathematical signals, the absorbance of the first detection hole is A1, the absorbance of the second detection hole is A2, the first detection hole is an auxiliary detection hole for eliminating the interference of the endogenous amino acid in the detection sample,
according to the detection principle of the glycated albumin, the data obtained by using A2-A1 is the absorbance of the glycated albumin, and then the concentration of the glycated albumin is calculated through a calibration curve of the absorbance and the concentration;
the absorbance of the third detection hole is used as A3, and then the concentration of albumin is calculated through a calibration curve of the absorbance and the concentration; then by the formula:
glycated Albumin (GA)% (concentration of glycated albumin/concentration of albumin × 100%)/1.14 +2.9
And calculating the ratio of the glycated albumin to the total protein.
Compared with the prior art, the centrifugal microfluidic chip for detecting the glycated albumin and the using method thereof have the following advantages:
(1) the centrifugal micro-fluidic chip for detecting the glycated albumin and the use method thereof have the advantages of simple chip structure, convenient realization, compact structure and high space utilization rate, and the glycated albumin is detected on the centrifugal micro-fluidic chip.
(2) According to the centrifugal microfluidic chip for detecting the glycated albumin and the using method thereof, the amount of a sample and a reaction reagent to be detected is only one tenth of the amount of a traditional large biochemical detection agent, so that the time required by detection is greatly shortened.
(3) According to the centrifugal micro-fluidic chip for detecting the glycated albumin and the using method thereof, the interference of the detection of the glycated albumin caused by the intrinsic glycated amino acid contained in the detection sample can be effectively solved through the arrangement of the auxiliary detection holes, so that the detection result of the glycated albumin is more stable.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate an embodiment of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic diagram of a centrifugal microfluidic chip for glycated albumin assay according to an embodiment of the present invention;
description of reference numerals:
1. a chip tray; 2. a sample transport unit; 21. a sample loading slot; 22. a sample quantification tank; 3. a diluent delivery unit; 31. a diluent storage tank; 32. a diluent transition groove; 33. a diluent quantitative tank; 4. a mixing tank; 5. a detection unit; 51. a first detection hole; 52. a second detection hole; 53. a third detection hole; 54. a fourth detection hole; 55. positioning holes; 6. a first siphon runner; 7. a second siphon runner; 8. and a third siphon flow channel.
Detailed Description
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.
A centrifugal micro-fluidic chip for glycated albumin detection: including chip dish 1, 1 surface of chip dish is equipped with sample conveying unit 2, diluent conveying unit 3, mixing tank 4 and detecting element 5, detecting element 5 sets up the edge at chip dish 1, mixing tank 4 sets up between diluent conveying unit 3 and detecting element 5, diluent conveying unit 3's diluent dosage tank 33 is connected to mixing tank 4 through a siphon runner 6, sample conveying unit 2's sample dosage tank 22 is connected to mixing tank 4 through No. two siphon runners 7, mixing tank 4 is connected to detecting element 5 through No. three siphon runners 8.
The chip tray 1 is integrally in a fan-shaped structure, the structural design can realize the free combination of a plurality of fan-shaped chips on the detection platform in the process of one-time detection, and one chip can detect one sample, so that the simultaneous detection of a plurality of samples and a plurality of detection items can be realized by one-time detection; the invention is not limited to the design of the fan-shaped structure, and the angle of the fan-shaped structure can be designed differently according to the requirement, and can also be a semicircular structure or a full-circle structure;
the diluent conveying unit 3 comprises a diluent storage tank 31, a diluent transition tank 32 and a diluent quantitative tank 33, wherein the diluent storage tank 31, the diluent transition tank 32 and the diluent quantitative tank 33 are sequentially arranged on the chip disc 1 from the direction of the inner arc and the outer arc, the diluent storage tank 31 is communicated to the diluent transition tank 32 through a first micro-channel, and the diluent transition tank 32 is communicated to the diluent quantitative tank 33 through a second micro-channel; because the diluent bag generally stores the diluent with larger volume, the transition groove structure is added in the middle, thus the flow of the fluid entering the diluent quantitative groove 33 can be controlled, the inaccurate quantitative volume caused by the bubbles generated in the quantitative process of the diluent is avoided,
the sample conveying unit 2 comprises a sample loading slot 21 and a sample quantifying slot 22, the chip tray 1 is sequentially provided with the sample loading slot 21 and the sample quantifying slot 22 from the direction of the inner arc to the outer arc, and the sample loading slot 21 is connected to the sample quantifying slot 22 through a third micro-channel.
The detection unit 5 comprises a plurality of detection holes and a plurality of positioning holes 55, the detection holes and the positioning holes 55 are uniformly distributed on the chip tray 1 in a manner of being matched with the outer arc of the chip tray 1, in the embodiment, the number of the detection holes is 7, one positioning hole 55 is arranged between every two detection holes, the positioning holes 55 can be matched with the detection position of a biochemical immunization machine when the chip tray 1 is placed on the biochemical immunization machine, wherein 4 detection holes close to the mixing tank 4 are communicated to the mixing tank 4 through a flow channel, and the 4 detection holes are sequentially used as a first detection hole 51, a second detection hole 52, a third detection hole 53 and a fourth detection hole 54 from left to right; more than 4 detection holes can be arranged, so that more detection items can be placed conveniently, and the joint detection of a plurality of detection items can be realized;
the first freeze-drying reagent is arranged in the first detection hole 51, is a detection reagent for endogenous glycated amino acids, mainly comprises ketoamine oxidase or fructosyl oxidase, catalase and a color source material, and is also provided with auxiliary materials, excipients and a protective agent.
The second freeze-drying reagent is arranged in the second detection hole 52, is a detection reagent of glycated albumin, and mainly comprises proteolytic enzyme, ketoamine oxidase, catalase, a color source material, fructosyl amino acid oxidase, and is also provided with auxiliary materials, an excipient and a protective agent.
A third freeze-drying reagent is arranged in the third detection hole 53, the third freeze-drying reagent is an albumin detection reagent, the main component of the third freeze-drying reagent is bromocresol green, and auxiliary materials, excipients and protective agents are further arranged.
The excipient is inert substance, including sucrose, glucose, trehalose, melezitose, dextran and mannitol, and polymer PEG and PVP; the protective agent is protein, including BSA, gelatin, and collagen.
The width of the first micro-channel, the second micro-channel and the third micro-channel is 0.01-1mm, the depth of the first micro-channel, the second micro-channel and the third micro-channel is 0.01-1mm, the inner surfaces of the first siphon channel 6 and the second siphon channel 7 are hydrophilic surfaces, and the inner surfaces of the first siphon channel 6 and the second siphon channel 7 are made to have hydrophilicity through one treatment mode of plasma surface treatment, ozone radiation treatment, surfactant treatment and graft copolymerization treatment.
A centrifugal microfluidic chip for glycated albumin detection and a using method thereof comprise the following steps:
s1, placing the packaged diluent sac into the diluent storage tank 31, adding the detection sample into the sample adding slot 21, and then placing the chip tray 1 on a detection platform of a biochemical immune machine for fixation;
s2, controlling the biochemical immune machine to start, opening the diluent bag, rotating the motor of the biochemical immune machine, enabling the diluent to enter the diluent quantifying groove 33 to complete quantification, and enabling the sample to enter the sample quantifying groove 22 to complete quantification of the sample; the time for the motor to rotate is 10-300 s;
s3, stopping the rotation of the motor, and opening the first siphon flow channel 6 and the second siphon flow channel 7 due to the hydrophilicity of the first siphon flow channel 6 and the second siphon flow channel 7; the motor is started, the diluent in the diluent quantifying groove 33 enters the mixing groove 4 through the first siphon flow channel 6 under the action of centrifugal force generated by the rotation of the motor, and the sample in the sample quantifying groove 22 enters the mixing groove 4 through the second siphon flow channel 7 under the action of centrifugal force generated by the rotation of the motor;
s4, the sample and the diluent enter the mixing tank 4, and the motor is controlled to rotate at the acceleration and deceleration moment to generate a vibration effect so that the sample and the diluent are fully mixed;
s5, stopping rotating the motor, opening the third siphon flow channel 8, enabling a mixed solution of the sample and the diluent to sequentially enter the first detection hole 51, the second detection hole 52, the third detection hole 53 and the fourth detection hole 54 through the third siphon flow channel 8 under the action of centrifugal force, starting the motor, and performing acceleration and deceleration rotation to enable the mixed solution to respectively react with reagents in the first detection hole 51, the second detection hole 52, the third detection hole 53 and the fourth detection hole 54;
s6, after the reaction is finished, the optical module of the detection platform detects the first detection hole 51, the second detection hole 52, the third detection hole 53 and the fourth detection hole 54, the photoelectric conversion module converts the optical signals in the first detection hole 51, the second detection hole 52, the third detection hole 53 and the fourth detection hole 54 into mathematical signals, the absorbance of the first detection hole 51 is A1, the absorbance of the second detection hole 52 is A2, wherein the first detection hole 51 is an auxiliary detection hole for eliminating the interference of endogenous amino acids in the detection sample,
according to the detection principle of the glycated albumin:
(1) measuring the glycated albumin;
a. in a detected sample (blood serum or blood plasma), firstly, saccharified amino acid oxidase (ketoamine oxidase) is injected to react to change endogenous saccharified amino acid into glucose ketoaldehyde, amino acid and hydrogen peroxide for removal;
b. injecting protease specific to albumin into the treatment solution, and saccharifying albumin to generate saccharified amino acid after the protease acts;
c. injecting saccharified amino acid oxidase to produce glucoketoaldehyde, amino acid and hydrogen peroxide;
d. hydrogen peroxide and a chromogenic substrate generate a chromogenic substance under the action of peroxidase, and the color depth is in direct proportion to the concentration of the glycated albumin;
the reaction equation is as follows:
(2) measuring albumin;
in a solution with the pH of 4.2, albumin and bromocresol green can generate a blue mixture, and the shade of the color is in direct proportion to the concentration of the albumin;
(3) calculating the ratio of glycated albumin to total protein;
according to the four equations in the glycated albumin determination, the data obtained by using A2-A1 is the absorbance of the glycated albumin after the interference of the endogenous glycated amino acid is subtracted, and then the concentration of the glycated albumin is calculated through a calibration curve of the absorbance and the concentration;
the absorbance of the third detection hole 53 is used as A3, and then the concentration of albumin is calculated through a calibration curve of the absorbance and the concentration; then by the formula:
glycated Albumin (GA)% (concentration of glycated albumin/concentration of albumin × 100%)/1.14 +2.9
And calculating the ratio of the glycated albumin to the total protein. The centrifugal micro-fluidic chip for detecting the glycated albumin and the use method thereof provided by the invention have the advantages that the chip is simple in structure and convenient to realize, and the interference of endogenous amino acid contained in a detected sample on the glycated albumin can be effectively removed.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A centrifugal micro-fluidic chip for glycated albumin assay, characterized in that: including the chip dish, chip dish surface is equipped with sample transport unit, diluent transport unit, mixing tank and detecting element, and the detecting element sets up the edge at the chip dish, and the mixing tank setting is between diluent transport unit and detecting element, and diluent transport unit's diluent ration groove is connected to the mixing tank through a siphon runner, and sample transport unit's sample ration groove is connected to the mixing tank through No. two siphon runners, and the mixing tank is connected to the detecting element through No. three siphon runners.
2. The centrifugal microfluidic chip for glycated albumin assay as claimed in claim 1, wherein: the whole fan-shaped structure that is of chip dish, diluent conveying unit include diluent holding tank, diluent cross the aqueduct, diluent ration groove, and the chip dish sets gradually diluent holding tank, diluent cross the aqueduct, diluent ration groove from the direction of inner arc outer arc, and the diluent holding tank communicates through a microchannel to the diluent and crosses the aqueduct, and the diluent passes the aqueduct and communicates through No. two microchannels to the diluent ration groove.
3. The centrifugal microfluidic chip for glycated albumin assay as claimed in claim 1, wherein: the sample conveying unit comprises a sample loading slot and a sample quantifying slot, the chip tray is sequentially provided with the sample loading slot and the sample quantifying slot from the direction of the inner arc to the outer arc, and the sample loading slot is connected to the sample quantifying slot through a third micro-channel.
4. The centrifugal microfluidic chip for glycated albumin assay as claimed in claim 3, wherein: the detecting unit comprises a plurality of detecting holes and a plurality of positioning holes, the detecting holes and the positioning holes are matched with the outer arc of the chip tray and are uniformly distributed on the chip tray, one positioning hole is arranged between every two detecting holes, 4 detecting holes close to the mixing tank are communicated to the mixing tank through a flow channel, and the 4 detecting holes are sequentially used as a first detecting hole, a second detecting hole, a third detecting hole and a fourth detecting hole from left to right.
5. The centrifugal microfluidic chip for glycated albumin assay as claimed in claim 4, wherein: the first freeze-drying reagent is a detection reagent for endogenous glycated amino acids, and mainly comprises ketoamine oxidase or fructosyl oxidase, catalase, a color source material, and auxiliary materials, an excipient and a protective agent.
6. The centrifugal microfluidic chip for glycated albumin assay as claimed in claim 4, wherein: the second freeze-drying reagent is a glycated albumin detection reagent, and mainly comprises proteolytic enzyme, ketoamine oxidase, catalase, a color source material, fructosyl amino acid oxidase, and auxiliary materials, an excipient and a protective agent.
7. The centrifugal microfluidic chip for glycated albumin assay as claimed in claim 4, wherein: a third freeze-drying reagent is arranged in the third detection hole, the third freeze-drying reagent is an albumin detection reagent, the main component is bromocresol green, and auxiliary materials, excipients and protective agents are further arranged.
8. The centrifugal microfluidic chip for glycated albumin assay as claimed in claim 7, wherein: the excipient is inert substance, including sucrose, glucose, trehalose, melezitose, dextran and mannitol, and polymer PEG and PVP; the protective agent is protein, including BSA, gelatin, and collagen.
9. The centrifugal microfluidic chip for glycated albumin assay as claimed in claim 3, wherein: the width of the first micro-channel, the second micro-channel and the third micro-channel is 0.01-1mm, and the depth is 0.01-1 mm; the inner surfaces of the first siphon runner and the second siphon runner are hydrophilic surfaces.
10. A method of using the centrifugal microfluidic chip for glycated albumin assay of claims 1-9, comprising the steps of:
s1, placing the packaged diluent sac into a diluent storage tank, adding a detection sample into a sample adding slot, and then placing the chip tray on a detection platform of a biochemical immune machine for fixation;
s2, controlling the biochemical immune machine to start, opening the diluent bag, rotating the motor of the biochemical immune machine, enabling the diluent to enter the diluent quantifying groove to complete quantification, and enabling the sample to enter the sample quantifying groove to complete quantification of the sample; the time for the motor to rotate is 10-300 s;
s3, stopping the rotation of the motor, and opening the first siphon flow passage and the second siphon flow passage due to the hydrophilicity of the first siphon flow passage and the second siphon flow passage; the motor is started, the diluent in the diluent quantifying groove enters the mixing groove through the first siphon flow channel under the action of centrifugal force generated by the rotation of the motor, and the sample in the sample quantifying groove enters the mixing groove through the second siphon flow channel under the action of centrifugal force generated by the rotation of the motor;
s4, the sample and the diluent enter a mixing tank, and the motor is controlled to rotate at the acceleration and deceleration moment to generate a vibration effect so that the sample and the diluent are fully mixed;
s5, stopping rotation of the motor, opening the third siphon flow channel, enabling a mixed solution of the sample and the diluent to sequentially enter the first detection hole, the second detection hole, the third detection hole and the fourth detection hole through the third siphon flow channel under the action of centrifugal force, starting the motor, and performing acceleration and deceleration rotation to enable the mixed solution to respectively react with reagents in the first detection hole, the second detection hole, the third detection hole and the fourth detection hole;
s6, after the reaction is finished, the optical module of the detection platform detects the first detection hole, the second detection hole, the third detection hole and the fourth detection hole, the photoelectric conversion module converts the optical signals in the first detection hole, the second detection hole, the third detection hole and the fourth detection hole into mathematical signals, the absorbance of the first detection hole is A1, the absorbance of the second detection hole is A2, the first detection hole is an auxiliary detection hole for eliminating the interference of the endogenous amino acid in the detection sample,
according to the detection principle of the glycated albumin, the data obtained by using A2-A1 is the absorbance of the glycated albumin, and then the concentration of the glycated albumin is calculated through a calibration curve of the absorbance and the concentration;
the absorbance of the third detection hole is used as A3, and then the concentration of albumin is calculated through a calibration curve of the absorbance and the concentration; then by the formula:
the ratio of Glycated Albumin (GA)% (concentration of glycated albumin/concentration of albumin × 100%)/1.14 +2.9 was calculated as the ratio of glycated albumin to total protein.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113546703A (en) * | 2021-07-30 | 2021-10-26 | 苏州含光微纳科技有限公司 | Centrifugal micro-fluidic chip |
| WO2022217548A1 (en) * | 2021-04-15 | 2022-10-20 | 天津纳百芯科技有限公司 | Microfluidic substrate, microfluidic chip, and operating method thereof |
| WO2023071365A1 (en) * | 2021-10-27 | 2023-05-04 | 含光微纳科技(太仓)有限公司 | Biochemical item detection device |
| WO2024130476A1 (en) * | 2022-12-19 | 2024-06-27 | 深圳迈瑞动物医疗科技股份有限公司 | Centrifugal microfluidic biochemistry analyzer and concentration measurement system |
| WO2024138383A1 (en) * | 2022-12-27 | 2024-07-04 | 深圳迈瑞动物医疗科技股份有限公司 | Microfluidic biochemical test disc and test method therefor |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201110454D0 (en) * | 2011-06-21 | 2011-08-03 | College The | Microfluidic photoporation |
| CN110508335A (en) * | 2019-03-27 | 2019-11-29 | 广州万孚生物技术股份有限公司 | Micro-fluidic chip and vitro detection device containing the micro-fluidic chip |
| CN110777049A (en) * | 2019-11-12 | 2020-02-11 | 北京金豪制药股份有限公司 | Microfluidic chip, nucleic acid detector and nucleic acid detection method |
| CN110872558A (en) * | 2019-12-13 | 2020-03-10 | 天津诺迈科技有限公司 | Micro-fluidic chip system for light-activated chemiluminescence and molecular detection and use method thereof |
| CN214794404U (en) * | 2020-12-29 | 2021-11-19 | 天津诺迈科技有限公司 | Centrifugal micro-fluidic chip for glycated albumin detection |
-
2020
- 2020-12-29 CN CN202011607490.3A patent/CN112577902A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201110454D0 (en) * | 2011-06-21 | 2011-08-03 | College The | Microfluidic photoporation |
| CN110508335A (en) * | 2019-03-27 | 2019-11-29 | 广州万孚生物技术股份有限公司 | Micro-fluidic chip and vitro detection device containing the micro-fluidic chip |
| CN110777049A (en) * | 2019-11-12 | 2020-02-11 | 北京金豪制药股份有限公司 | Microfluidic chip, nucleic acid detector and nucleic acid detection method |
| CN110872558A (en) * | 2019-12-13 | 2020-03-10 | 天津诺迈科技有限公司 | Micro-fluidic chip system for light-activated chemiluminescence and molecular detection and use method thereof |
| CN214794404U (en) * | 2020-12-29 | 2021-11-19 | 天津诺迈科技有限公司 | Centrifugal micro-fluidic chip for glycated albumin detection |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022217548A1 (en) * | 2021-04-15 | 2022-10-20 | 天津纳百芯科技有限公司 | Microfluidic substrate, microfluidic chip, and operating method thereof |
| CN113546703A (en) * | 2021-07-30 | 2021-10-26 | 苏州含光微纳科技有限公司 | Centrifugal micro-fluidic chip |
| WO2023071365A1 (en) * | 2021-10-27 | 2023-05-04 | 含光微纳科技(太仓)有限公司 | Biochemical item detection device |
| WO2024130476A1 (en) * | 2022-12-19 | 2024-06-27 | 深圳迈瑞动物医疗科技股份有限公司 | Centrifugal microfluidic biochemistry analyzer and concentration measurement system |
| WO2024138383A1 (en) * | 2022-12-27 | 2024-07-04 | 深圳迈瑞动物医疗科技股份有限公司 | Microfluidic biochemical test disc and test method therefor |
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