CN112410209A - Temperature control system for chromosome harvesting equipment and automatic temperature control method - Google Patents
Temperature control system for chromosome harvesting equipment and automatic temperature control method Download PDFInfo
- Publication number
- CN112410209A CN112410209A CN202011350056.1A CN202011350056A CN112410209A CN 112410209 A CN112410209 A CN 112410209A CN 202011350056 A CN202011350056 A CN 202011350056A CN 112410209 A CN112410209 A CN 112410209A
- Authority
- CN
- China
- Prior art keywords
- temperature
- control system
- heating
- centrifugal
- temperature control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B13/00—Control arrangements specially designed for centrifuges; Programme control of centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
- B04B15/02—Other accessories for centrifuges for cooling, heating, or heat insulating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
- B04B5/0421—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q3/00—Condition responsive control processes
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Thermal Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a temperature control system for chromosome harvesting equipment, which belongs to the field of chromosome harvesting equipment and comprises a centrifugal module, a centrifugal cavity, a heating terminal and a temperature controller, wherein the centrifugal module comprises a plurality of oscillation centrifuges, the heating terminal is positioned at the periphery or the bottom of the centrifugal module, the centrifugal module and the heating terminal are both positioned in the centrifugal cavity, the temperature controller is electrically connected with the heating terminal, and the temperature controller controls the heating terminal to assist in heating the centrifugal cavity, so that each oscillation centrifuge on the centrifugal module is in a preset temperature working range. The temperature control system for the chromosome harvesting equipment disclosed by the invention not only can maintain the constant temperature of the equipment in the whole harvesting process, especially the constant temperature in the hypotonic step, but also can realize the full automation of the whole temperature control process, and is beneficial to harvesting high-quality chromosomes.
Description
Technical Field
The invention relates to the field of chromosome harvesting equipment, in particular to an automatic temperature control method for a temperature control system for chromosome harvesting equipment.
Background
The chromosome harvesting equipment can automatically harvest metaphase chromosomes of peripheral blood, bone marrow, amniotic fluid, umbilical cord blood, villus cells and other samples, and has wide application in the fields of cell culture, metaphase chromosomes and other biological medicines. Chromosome harvesting equipment often needs to carry out operations such as centrifugation, supernatant suction, hypotonic solution addition, liquid fixing and oscillation, and the existing chromosome harvesting equipment is difficult to further implement constant-temperature harvesting due to the fact that the whole chromosome harvesting equipment is difficult to place in a constant-temperature environment, and further cannot carry out automatic constant-temperature control in a hypotonic step.
Chinese patent publication No. CN209117705U discloses a harvesting processing system for full-automatic chromosome harvesting equipment, which comprises a liquid supply mechanism, a liquid injection mechanism, a liquid suction mechanism, a flushing liquid suction needle mechanism, a centrifugal mechanism and an oscillation mechanism, wherein the centrifugal mechanism comprises a rotary turntable, a test tube bracket, a rotary driving mechanism and an oscillation mechanism; the flushing imbibition needle mechanism comprises an imbibition pump, an imbibition pool and a blowing mechanism, the blowing mechanism comprises a blowing pump and a blowing pipe, the imbibition mechanism comprises an imbibition pump, an imbibition needle and a waste liquid barrel, and the blowing pipe is arranged on the imbibition pool. The centrifugal mechanism of the harvesting processing system provided by the above patent is continuously rotated, and it is difficult to directly heat the harvesting processing system, so that the existing harvesting processing system is difficult to maintain a constant temperature state in the whole harvesting process.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a temperature control system for chromosome harvesting equipment, which can maintain the equipment at a constant temperature in the whole harvesting process, particularly at a constant temperature in a hypotonic step, can realize full automation of the whole temperature control process, and is beneficial to harvesting high-quality chromosomes.
In order to achieve the purpose, the invention adopts the following technical scheme:
the temperature control system for the chromosome harvesting equipment comprises a centrifugal module, a centrifugal cavity, a heating terminal and a temperature controller, wherein the centrifugal module comprises a plurality of oscillating centrifuges, the heating terminal is positioned at the periphery or the bottom of the centrifugal module, the centrifugal module and the heating terminal are both positioned in the centrifugal cavity, the temperature controller is electrically connected with the heating terminal, and the temperature controller controls the heating terminal to assist in heating the centrifugal cavity, so that each oscillating centrifuge on the centrifugal module is in a preset temperature working range.
The technical scheme of the invention is that the heating terminal is adjusted by the temperature controller according to the temperature signals of different oscillation centrifuges to assist heating.
A further technical solution of the present invention is that the heating terminal is configured as a heating ring; or
The heating terminal is configured into a plurality of heating plates, the heating plates correspond to the plurality of oscillating centrifuges, and each heating plate is positioned at one side or the bottom of the corresponding oscillating centrifuges; or
The heating terminal is configured to be a plurality of heating wire coils, and each heating wire coil is wound on the corresponding oscillation centrifuge or forms a wire coil to be positioned at the bottom of the oscillation centrifuge.
The further technical scheme of the invention is that the centrifugal vibration centrifugal machine further comprises a plurality of baffles and supporting plates, the baffles are connected end to form a protective frame, the baffles are fixed on the supporting plates, the centrifugal module is positioned in the protective frame, and the heating terminal is positioned between the protective frame and the baffles or below the centrifugal module and is matched with the corresponding vibration centrifugal machine.
The technical scheme is that a top sealing cover is arranged above the protection frame, the top sealing cover covers an upper opening of the protection frame, and the top sealing cover, the support plate and the protection frame enclose the centrifugal cavity.
The invention has the further technical scheme that a liquid adding mechanism and a liquid absorbing mechanism are arranged above the centrifugal module, a liquid adding hole array and a liquid absorbing hole array are arranged above the top sealing cover, the liquid adding hole array is matched with a liquid adding needle array in the liquid adding mechanism, and the liquid absorbing hole array is matched with a liquid absorbing needle array in the liquid absorbing mechanism.
The invention further adopts the technical scheme that the top sealing cover is provided with an observation port and a window plate for covering the observation port, the window plate is hinged with one side edge of the observation port, and a handle for opening and closing the window plate is arranged on the window plate.
The technical scheme of the invention is that the centrifugal module also comprises a rotating disk and a plurality of clamping devices fixed on the rotating disk, and the oscillating centrifuges containing the test tube sleeve arrays are fixed on the corresponding clamping devices.
The further technical scheme of the invention is that the temperature controller controls the temperature in the centrifugal cavity to be 37 +/-0.5 ℃; and/or the temperature controller is configured as a temperature control unit of a central control system of the chromosome harvesting device.
The invention also provides an automatic temperature control method for the temperature control system for the chromosome harvesting equipment, which is implemented according to the following steps:
s00: a central control system of the chromosome harvesting equipment sends a first temperature control instruction to the heating terminal, and the heating terminal heats the temperature in the centrifugal cavity to 37 +/-0.5 ℃;
s10: the central control system sends a liquid adding instruction to the liquid adding mechanism, and the liquid adding mechanism sequentially adds liquid to each oscillating centrifuge on the centrifugal module one by one;
s20: the chromosome harvesting equipment enters a chromosome harvesting stage;
s30: after the chromosome harvesting stage is completed, the central control system sends a liquid suction instruction to the liquid suction mechanism, and the liquid suction mechanism sequentially performs liquid suction operation on the oscillation centrifuges on the centrifugal module one by one;
s40: and the central control system of the chromosome harvesting equipment sends a second temperature control instruction to the heating terminal, and the heating terminal stops heating.
The invention has the beneficial effects that:
according to the temperature control system for the chromosome harvesting equipment, the centrifugal module and the heating terminal are arranged in the centrifugal cavity, and the centrifugal cavity is subjected to auxiliary heating, so that the centrifugal module is placed in a constant-temperature harvesting environment with the temperature of 37 +/-0.5 ℃, the temperature of the constant-temperature harvesting environment is closer to the body temperature of a human body or an animal and closer to the ideal temperature for maintaining chromosome activity, and the chromosome harvesting equipment can realize full-process automatic constant-temperature harvesting, especially constant temperature in a hypotonic step. The temperature control system realizes closed-loop control of the temperature in the centrifugal cavity through the temperature collector, and can effectively maintain the temperature in the centrifugal cavity to be stable. In addition, the chromosome harvesting equipment can completely and automatically implement the whole cell constant-temperature harvesting process by the creative defect supplementation, so that the product quality and the yield of metaphase chromosomes are further improved.
Drawings
FIG. 1 is a schematic structural view of a chromosome harvesting apparatus provided in an embodiment of the present invention;
FIG. 2 is a partial schematic view of a chromosome harvesting apparatus provided in an embodiment of the invention;
FIG. 3 is a perspective view of a temperature control system provided in an embodiment of the present invention;
FIG. 4 is a top view of a temperature control system provided in an embodiment of the present invention;
fig. 5 is a functional block diagram of a circuit of a temperature control system provided in an embodiment of the present invention.
In the figure:
1. a centrifuge module; 2. a centrifugal chamber; 3. heating the terminal; 4. a temperature controller; 11. oscillating a centrifuge; 5. a temperature collector; 61. a baffle plate; 62. a support plate; 6. a protective frame; 63. a top sealing cover; 7. a liquid adding mechanism; 8. a liquid suction mechanism; 631. a liquid adding hole array; 632. an array of pipetting holes; 71. a liquid adding needle array; 81. an array of pipette needles; 633. a window panel; 634. a handle; 12. rotating the disc; 13. a holder; 14. a drive mechanism.
Detailed Description
The technical scheme of the invention is further explained by the specific implementation mode in combination with the attached drawings.
Example one
As shown in fig. 1 to 5, the temperature control system for chromosome harvesting equipment provided by the present invention includes a centrifugal module 1 including a plurality of oscillation centrifuges 11, a centrifugal cavity 2, a heating terminal 3 and a temperature controller 4, wherein the heating terminal 3 is located at the periphery or bottom of the centrifugal module 1, the centrifugal module 1 and the heating terminal 3 are both located in the centrifugal cavity 2, the temperature controller 4 is electrically connected to the heating terminal 3, and the temperature controller 4 controls the heating terminal 3 to supplement heat to the centrifugal cavity 2, so that each oscillation centrifuges 11 on the centrifugal module 1 are within a preset temperature working range. Centrifugal cavity 2 is seal chamber, through arranging centrifugal module 1 in seal chamber, in seal chamber was arranged in to the vibration centrifuge 11 that will each install on indirect test tube array to the realization is to the long-time constant temperature state that maintains of test tube array internal cell suspension, can maintain heating terminal 3 all the time through temperature controller 4 and carry out stable auxiliary heating, and timely temperature variation reacts.
In order to accurately control the temperature of the test tube array in each oscillation centrifuge 11 in the sealed cavity in a closed loop manner, furthermore, a plurality of temperature collectors 5 are arranged on the centrifuge module 1, the temperature collectors 5 respectively collect the temperature signals of the corresponding oscillation centrifuges 11, and transmit the temperature signals of different oscillation centrifuges 11 to the temperature controller 4, and the temperature controller 4 adjusts the heating terminal 3 to assist in heating according to the temperature signals of different oscillation centrifuges 11. A plurality of temperature collector 5 gather the temperature signal of different vibration centrifuges 11, also indirectly gather test tube array's temperature signal, and convey temperature signal to temperature controller 4 in real time, temperature controller 4 can be according to the temperature signal rational adjustment heating terminal 3 calorific capacity of different positions of different vibration centrifuges 11, in order to reach the accurate accuse temperature to different test tube arrays, make the temperature of the different test tube arrays of constant temperature harvest environment all be closer to human or animal's body temperature, the ideal temperature that keeps chromosome activity is pressed close to more, thereby make chromosome harvesting equipment can realize full process automation constant temperature harvest, and then promote the quality and the productivity of cell, the ideal temperature of chromosome activity is about 37 ℃ +/-0.5 ℃.
In order to assist the heat respectively in each vibration centrifuge 11 test tube array in seal chamber, furtherly, heating terminal 3 disposes into a plurality of hot plates, a plurality of hot plates are corresponding with a plurality of vibration centrifuges 11, every hot plate all is located one side or the bottom of corresponding vibration centrifuge 11, thereby assist the heat to different vibration centrifuges 11, temperature controller 4 assists the heat to different vibration centrifuges 11 through controlling different hot plates respectively, just can reach effectual temperature of guaranteeing each different test tube array and be in stable state for a long time, reach independent control and accurate control to different vibration centrifuges 11 and assist the heat.
The temperature control system for the chromosome harvesting equipment further comprises a plurality of baffles 61 and a support plate 62, the baffles 61 are connected end to form a protection frame 6, the baffles 61 are fixed on the support plate 62, the baffles 61 are all perpendicular to the support plate 62, the number of the baffles 61 is generally consistent with the number of the oscillation centrifuges 11, for example, eight oscillation centrifuges 11 are disclosed in the drawing, and the number of the corresponding baffles 61 is eight. The centrifuge module 1 is located in a protective frame 6, and the heating terminals 3 are located between the protective frame 6 and the baffle 61 or below the centrifuge module 1 and are adapted to the respective oscillating centrifuge 11. The protective frame 6 can protect the centrifugal module 1, is an important component of the centrifugal cavity 2 and can form a heat-preservation cavity.
In order to form the sealed centrifugal chamber 2, a top cover 63 that can be closed and opened is provided above the protective frame 6, the top cover 63 covers the upper opening of the protective frame 6, and the top cover 63, the support plate 62, and the protective frame 6 enclose the centrifugal chamber 2. Because the top sealing cover 63 and the supporting plate 62 respectively seal the openings at the upper end and the lower end of the protective frame 6, the centrifugal module 1 is arranged in the closed centrifugal cavity 2, and a constant temperature foundation is laid for implementing the full-process automatic constant temperature harvesting of chromosome harvesting equipment, especially the constant temperature in the hypotonic step.
In order to close the top of the centrifugal module 1 by the top sealing cover 63, a hole needs to be opened on the top sealing cover 63 so that the liquid adding mechanism 7 and the liquid absorbing mechanism 8 can add liquid and absorb liquid to the test tube arrays in different oscillation centrifuges 11 on the centrifugal module 1, further, the liquid adding mechanism 7 and the liquid absorbing mechanism 8 are arranged above the centrifugal module 1, a liquid adding hole array 631 and a liquid absorbing hole array 632 are arranged above the top sealing cover 63, the liquid adding hole array 631 is matched with the liquid adding needle array 71 in the liquid adding mechanism 7, and the liquid absorbing hole array 632 is matched with the liquid adding needle array 81 in the liquid absorbing mechanism 8. The liquid adding needle array 71 in the liquid adding mechanism 7 is inserted into the corresponding test tube array in the oscillation centrifuge 11 from the liquid adding hole array 631, and liquid adding operation is performed on each test tube of the test tube array. Accordingly, the pipette needle array 81 in the pipette mechanism 8 is inserted from the pipette well array 632 into the corresponding tube array in the oscillation centrifuge 11, and the respective tubes of the tube array are subjected to the pipette operation.
In order to facilitate observation of the working condition in the centrifugal chamber 2, the top cover 63 is provided with an observation port and a window plate 63 covering the observation port, the window plate 63 is hinged to one side edge of the observation port, and the window plate 63 is provided with a handle 64 for opening and closing the window plate 63. Can open luffer boards 63 through pulling handle 64, the user can be through the behavior in the centrifugation cavity 2 of viewing aperture, and of course top closing cap 63 also can be dismantled and opened, but it is dismantled and open relatively troublesome, because it itself has sealed requirement, just can the person of facilitating the use learn sealed cavity's intracavity situation rapidly through luffer boards 63, easy operation is convenient.
The centrifugal module 1 further comprises a rotating disc 12 and a plurality of holders 13 fixed on the rotating disc 12, wherein each oscillating centrifuge 11 comprising a test tube sleeve array is fixed on the corresponding holder 13, a driving mechanism 14 is arranged at the bottom of the rotating disc 12, and a power output shaft of the driving mechanism 14 penetrates through the supporting plate 62 and is fixedly connected with the rotating disc 12, so that the rotating disc 12 is driven to rotate, the centrifugal module 1 in the centrifugal cavity 2 is driven to rotate, and the plurality of oscillating centrifuges 11 complete the centrifugal operation.
Further preferably, the temperature controller 4 controls the temperature in the centrifugal chamber 2 to be within a temperature range of 37 ℃. + -. 0.5 ℃ which is close to the body temperature of the human or animal body, more closely to the ideal temperature for maintaining the chromosome activity. Preferably, the temperature controller 4 is configured as a temperature control unit of a central control system of the chromosome harvesting apparatus, so that the temperature control unit shares hardware resources of the central control system. The temperature controller 4 can be independently set so as to adopt the high-sensitivity independently working temperature controller 4, and can also be directly embedded into a central control system of the chromosome harvesting equipment to be used as a temperature control unit to send out a temperature control instruction.
Example two
The temperature control system for chromosome harvesting equipment provided in this embodiment, including centrifugal module 1 that contains a plurality of vibration centrifuges 11, centrifugal cavity 2, heating terminal 3 and temperature controller 4, heating terminal 3 is located the periphery or the bottom of centrifugal module 1, centrifugal module 1 and heating terminal 3 all are located centrifugal cavity 2, temperature controller 4 is connected with heating terminal 3 electricity, temperature controller 4 control heating terminal 3 assists heat to centrifugal cavity 2, thereby make each vibration centrifuges 11 on the centrifugal module 1 all be in predetermineeing temperature working range.
The second embodiment is different from the first embodiment in that:
the heating terminals 3 are configured as heating rings which are located at the bottom of the centrifuge module 1 or in the space between the centrifuge module 1 and the protective frame 6 or are fixed to the inner wall of the protective frame 6. The heating ring has the main characteristics that the centrifugal cavity 2 can be subjected to integral auxiliary heating, the heating consistency is better, and the test tube arrays in different oscillation centrifuges 11 are difficult to realize independent accurate temperature control.
EXAMPLE III
The temperature control system for chromosome harvesting equipment provided in this embodiment, including centrifugal module 1 that contains a plurality of vibration centrifuges 11, centrifugal cavity 2, heating terminal 3 and temperature controller 4, heating terminal 3 is located the periphery or the bottom of centrifugal module 1, centrifugal module 1 and heating terminal 3 all are located centrifugal cavity 2, temperature controller 4 is connected with heating terminal 3 electricity, temperature controller 4 control heating terminal 3 assists heat to centrifugal cavity 2, thereby make each vibration centrifuges 11 on the centrifugal module 1 all be in predetermineeing temperature working range.
The third embodiment is different from the first and second embodiments in that:
the heating terminals 3 are configured as several heating wire coils, each of which is wound on a corresponding oscillatory centrifuge 11 or is wound on the bottom of the oscillatory centrifuge 11. A plurality of heater strip coils are similar with the hot plate, and the two both can realize assisting heat respectively to each vibration centrifuge 11 pilot tube array in seal chamber. But the installation mode of the heating wire coil can be more diversified and flexible, and the heating wire coil can be wound on the outer surface of the heated object, so that the heating effect is higher.
Example four
The automatic temperature control method for the temperature control system for the chromosome harvesting equipment disclosed in the embodiment is implemented according to the following steps:
and step S00: a central control system of the chromosome harvesting equipment sends a first temperature control instruction to the heating terminal 3, and after receiving the first temperature control instruction, the heating terminal 3 heats the temperature in the centrifugal cavity 2 to 37 +/-0.5 ℃ for a long time, so that the cell suspension is in an optimal temperature state in a chromosome harvesting stage.
And step S10: the central control system sends a liquid adding instruction to the liquid adding mechanism 7, the liquid adding mechanism 7 sequentially carries out liquid adding operation on each oscillation centrifuge 11 on the centrifugal module 1 one by one, a liquid adding needle array 71 in the liquid adding mechanism 7 is inserted into a corresponding test tube array in the oscillation centrifuge 11 from a liquid adding hole array 631, liquid adding operation is carried out on each test tube of the test tube array, and after the liquid adding operation is completed, the liquid adding mechanism 7 resets the oscillation centrifuge 11.
And step S20: and (4) the chromosome harvesting equipment enters a chromosome harvesting stage to obtain cell chromosomes.
And step S30: after the chromosome harvesting stage is completed, the central control system sends a liquid suction instruction to the liquid suction mechanism 8, and the liquid suction mechanism 8 performs liquid suction operation on the oscillation centrifuges 11 on the centrifugal module 1 one by one in sequence to complete the waste liquid removing process. The pipette needle array 81 in the pipette mechanism 8 is inserted from the pipette well array 632 into the corresponding tube array in the oscillation centrifuge 11, and performs a pipette operation on each tube of the tube array.
And step S40: and the central control system of the chromosome harvesting equipment sends a second temperature control instruction to the heating terminal 3, and the heating terminal 3 stops heating after receiving the second temperature control instruction.
In conclusion, from the steps S00 to S40, it can be seen that the chromosome harvesting device is in a constant temperature state, which greatly affects the harvesting effect of chromosome harvesting, and the chromosome harvesting device provided by the invention can fully automatically implement the chromosome harvesting process, thereby further improving the product quality and yield of the chromosome harvesting device.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the spirit and scope of the invention. The present invention is not to be limited by the specific embodiments disclosed herein, and other embodiments that fall within the scope of the claims of the present application are intended to be within the scope of the present invention.
Claims (10)
1. A temperature control system for chromosome harvesting equipment, comprising:
the centrifugal module comprises a plurality of oscillating centrifuges and a centrifugal cavity;
heating the terminal; the heating terminal is positioned at the periphery or the bottom of the centrifugal module;
the centrifugal module and the heating terminal are both positioned in the centrifugal cavity;
a temperature controller; the temperature controller is electrically connected with the heating terminal;
the temperature controller controls the heating terminal to assist the centrifugal cavity body in heating, so that each oscillation centrifuge on the centrifugal module is in a preset temperature working range.
2. The temperature control system for chromosome harvesting equipment according to claim 1, and characterized in that:
the temperature collectors are arranged on the centrifugal module;
the temperature collectors respectively collect temperature signals of corresponding oscillation centrifuges and transmit the temperature signals of different oscillation centrifuges to the temperature controller, and the temperature controller adjusts the heating terminal to assist in heating according to the temperature signals of the different oscillation centrifuges.
3. The temperature control system for chromosome harvesting equipment according to claim 1 or 2, and characterized in that:
the heating terminal is configured as a heating ring; or
The heating terminal is configured into a plurality of heating plates, the heating plates correspond to the plurality of oscillating centrifuges, and each heating plate is positioned at one side or the bottom of the corresponding oscillating centrifuges; or
The heating terminal is configured to be a plurality of heating wire coils, and each heating wire coil is wound on the corresponding oscillation centrifuge or forms a wire coil to be positioned at the bottom of the oscillation centrifuge.
4. The temperature control system for chromosome harvesting equipment according to claim 1 or 2, and characterized in that:
the protective frame is characterized by also comprising a plurality of baffles and supporting plates, wherein the baffles are connected end to form the protective frame;
a plurality of baffle plates are fixed on the supporting plate;
the centrifugal module is located in the protective frame, and the heating terminal is located between the protective frame and the baffle or below the centrifugal module and is matched with the corresponding oscillation centrifuge.
5. The temperature control system for chromosome harvesting equipment according to claim 4, and characterized in that:
a top sealing cover is arranged above the protection frame;
the top cover covers an upper opening of the protection frame;
the centrifugal cavity is enclosed by the top sealing cover, the supporting plate and the protection frame.
6. The temperature control system for chromosome harvesting equipment according to claim 5, and characterized in that:
a liquid adding mechanism and a liquid absorbing mechanism are arranged above the centrifugal module;
a liquid feeding hole array and a liquid sucking hole array are arranged above the top sealing cover;
the liquid adding hole array is matched with a liquid adding needle array in the liquid adding mechanism;
the liquid suction hole array is matched with a liquid suction needle array in the liquid suction mechanism.
7. The temperature control system for chromosome harvesting equipment according to claim 5 or 6, and characterized in that: the top seal cover is provided with an observation port and a window plate covering the observation port;
the window board is hinged with one side edge of the observation port;
and the window plate is provided with a handle for opening and closing the window plate.
8. The temperature control system for chromosome harvesting equipment according to claim 1, and characterized in that:
the centrifugal module also comprises a rotating disk and a plurality of clampers fixed on the rotating disk;
each of the shaker centrifuges containing an array of test tube sockets is secured to a respective one of the holders.
9. The temperature control system for chromosome harvesting equipment according to claim 1, and characterized in that:
the temperature controller controls the temperature in the centrifugal cavity to be 37 +/-0.5 ℃; and/or
The temperature controller is configured as a temperature control unit of a central control system of the chromosome harvesting device.
10. An automated temperature control method for the temperature control system for chromosome harvesting equipment according to any one of claims 1 to 9, and is characterized by being implemented by the following steps:
s00: a central control system of the chromosome harvesting equipment sends a first temperature control instruction to the heating terminal, and the heating terminal heats the temperature in the centrifugal cavity to 37 +/-0.5 ℃;
s10: the central control system sends a liquid adding instruction to the liquid adding mechanism, and the liquid adding mechanism sequentially adds liquid to each oscillating centrifuge on the centrifugal module one by one;
s20: the chromosome harvesting equipment enters a chromosome harvesting stage;
s30: after the chromosome harvesting stage is completed, the central control system sends a liquid suction instruction to the liquid suction mechanism, and the liquid suction mechanism sequentially performs liquid suction operation on the oscillation centrifuges on the centrifugal module one by one;
s40: and the central control system of the chromosome harvesting equipment sends a second temperature control instruction to the heating terminal, and the heating terminal stops heating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011350056.1A CN112410209A (en) | 2020-11-26 | 2020-11-26 | Temperature control system for chromosome harvesting equipment and automatic temperature control method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011350056.1A CN112410209A (en) | 2020-11-26 | 2020-11-26 | Temperature control system for chromosome harvesting equipment and automatic temperature control method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112410209A true CN112410209A (en) | 2021-02-26 |
Family
ID=74842126
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011350056.1A Pending CN112410209A (en) | 2020-11-26 | 2020-11-26 | Temperature control system for chromosome harvesting equipment and automatic temperature control method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112410209A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115873707A (en) * | 2022-12-29 | 2023-03-31 | 北京大学第三医院（北京大学第三临床医学院） | Cell harvesting system |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD272961A3 (en) * | 1987-07-03 | 1989-11-01 | Akad Wissenschaften Ddr | CENTRIFUGE FOR THE EVOLUTION OF BIOLOGICAL MATERIAL |
| CN2609932Y (en) * | 2003-04-04 | 2004-04-07 | 季海生 | Chromosome preparation instrument |
| JP2007070227A (en) * | 2005-09-02 | 2007-03-22 | Nagoya Institute Of Technology | Artificial protein complex and use thereof |
| WO2007048832A1 (en) * | 2005-10-26 | 2007-05-03 | Genial Genetic Solutions Limited | Apparatus for processing biological material |
| JP2007185101A (en) * | 2006-01-11 | 2007-07-26 | Hitachi High-Technologies Corp | Biological sample-analyzing apparatus |
| EP2227559A1 (en) * | 2007-11-30 | 2010-09-15 | Corbett Research Pty Ltd | Thermal cycling device |
| CN202011879U (en) * | 2010-11-12 | 2011-10-19 | 天津市新药安全评价研究中心 | Preparation instrument for metaphase of cell chromosome of mammal |
| CN204154727U (en) * | 2014-11-04 | 2015-02-11 | 深圳市爱康生物科技有限公司 | Constant-temperature incubation centrifugal device |
| CN106119088A (en) * | 2016-06-07 | 2016-11-16 | 李琳 | Chromosome gathers in the crops instrument automatically |
| CN205731668U (en) * | 2016-05-16 | 2016-11-30 | 江西培康营养科技有限公司 | A kind of Fat centrifugal machine |
| CN207923568U (en) * | 2018-03-21 | 2018-09-28 | 深圳市鹏一医疗仪器有限公司 | Full-automatic liquid-based cell sample manufacturing dyes all-in-one machine |
| WO2019074455A2 (en) * | 2017-10-12 | 2019-04-18 | Ozcan Mustafa | The centrifuge device for platelet-rich fibrin and concentrated growth factor with photobiostimulation |
| CN209117705U (en) * | 2018-11-28 | 2019-07-16 | 佛山市艾达思精密仪器有限公司 | A kind of harvest processing system for full-automatic chromosome harvest equipment |
-
2020
- 2020-11-26 CN CN202011350056.1A patent/CN112410209A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD272961A3 (en) * | 1987-07-03 | 1989-11-01 | Akad Wissenschaften Ddr | CENTRIFUGE FOR THE EVOLUTION OF BIOLOGICAL MATERIAL |
| CN2609932Y (en) * | 2003-04-04 | 2004-04-07 | 季海生 | Chromosome preparation instrument |
| JP2007070227A (en) * | 2005-09-02 | 2007-03-22 | Nagoya Institute Of Technology | Artificial protein complex and use thereof |
| WO2007048832A1 (en) * | 2005-10-26 | 2007-05-03 | Genial Genetic Solutions Limited | Apparatus for processing biological material |
| JP2007185101A (en) * | 2006-01-11 | 2007-07-26 | Hitachi High-Technologies Corp | Biological sample-analyzing apparatus |
| EP2227559A1 (en) * | 2007-11-30 | 2010-09-15 | Corbett Research Pty Ltd | Thermal cycling device |
| CN202011879U (en) * | 2010-11-12 | 2011-10-19 | 天津市新药安全评价研究中心 | Preparation instrument for metaphase of cell chromosome of mammal |
| CN204154727U (en) * | 2014-11-04 | 2015-02-11 | 深圳市爱康生物科技有限公司 | Constant-temperature incubation centrifugal device |
| CN205731668U (en) * | 2016-05-16 | 2016-11-30 | 江西培康营养科技有限公司 | A kind of Fat centrifugal machine |
| CN106119088A (en) * | 2016-06-07 | 2016-11-16 | 李琳 | Chromosome gathers in the crops instrument automatically |
| WO2019074455A2 (en) * | 2017-10-12 | 2019-04-18 | Ozcan Mustafa | The centrifuge device for platelet-rich fibrin and concentrated growth factor with photobiostimulation |
| CN207923568U (en) * | 2018-03-21 | 2018-09-28 | 深圳市鹏一医疗仪器有限公司 | Full-automatic liquid-based cell sample manufacturing dyes all-in-one machine |
| CN209117705U (en) * | 2018-11-28 | 2019-07-16 | 佛山市艾达思精密仪器有限公司 | A kind of harvest processing system for full-automatic chromosome harvest equipment |
Non-Patent Citations (2)
| Title |
|---|
| 刘利兵等: "《实验基础医学(第三版)》", 31 July 2009, 第四军医大学出版社 * |
| 董烁等: "浅谈人体外周血淋巴细胞染色体的制备", 《食品工程》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115873707A (en) * | 2022-12-29 | 2023-03-31 | 北京大学第三医院（北京大学第三临床医学院） | Cell harvesting system |
| CN115873707B (en) * | 2022-12-29 | 2023-11-24 | 北京大学第三医院（北京大学第三临床医学院） | Cell harvesting system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN206396247U (en) | Band automatically controls the new incubator of oscillating uniform device | |
| CN108138114B (en) | Improvements in and relating to bio-manufacturing equipment | |
| CN112410209A (en) | Temperature control system for chromosome harvesting equipment and automatic temperature control method | |
| CN110676477B (en) | Automatic batch sample preparation method for button batteries | |
| CN117327564A (en) | Constant temperature rotary cell tissue dissociation instrument | |
| CN212713578U (en) | Batch cell resuscitator | |
| CN210127238U (en) | Food microorganism detection device | |
| CN210119387U (en) | Convenient dyeing box capable of uniformly dyeing samples in batches | |
| CN213924562U (en) | Electric automatization organic fertilizer fermenting installation | |
| CN213977725U (en) | Cell bio-genetic engineering is with bio-pharmaceuticals stem cell reaction equipment | |
| CN216513886U (en) | Solid state fermentation fodder scale production equipment | |
| CN206559948U (en) | A kind of soil animal collection device | |
| CN213924869U (en) | Cell culture device | |
| CN213975356U (en) | Portable box for blood sampling in clinical laboratory | |
| CN209715150U (en) | A kind of use for laboratory insulating utensils | |
| CN211713051U (en) | Porphyridium culture device | |
| CN219194979U (en) | Separation and screening device for lactobacillus production | |
| CN206706116U (en) | A kind of tissue digestion device | |
| CN110791431A (en) | Online ultrasonic stripping cell disruption bioreactor and virus harvesting method | |
| CN206887123U (en) | Cell draw-gear | |
| CN221254549U (en) | Constant temperature shaking table | |
| CN109550535A (en) | A kind of intelligent constant-temperature instrument | |
| CN217757383U (en) | Rice wine production is with constant temperature fermentation cylinder | |
| CN220831753U (en) | Green tea processing stir-frying device | |
| CN218981635U (en) | Agarose gel heater |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210226 |