CN112316727A - Bacterial seedling ultrafiltration concentration equipment and ultrafiltration concentration method - Google Patents
Bacterial seedling ultrafiltration concentration equipment and ultrafiltration concentration method Download PDFInfo
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- CN112316727A CN112316727A CN202011173182.4A CN202011173182A CN112316727A CN 112316727 A CN112316727 A CN 112316727A CN 202011173182 A CN202011173182 A CN 202011173182A CN 112316727 A CN112316727 A CN 112316727A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/18—Apparatus therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/20—Accessories; Auxiliary operations
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
- B01D63/02—Hollow fibre modules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/10—Centrifuges combined with other apparatus, e.g. electrostatic separators; Sets or systems of several centrifuges
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Abstract
The invention discloses a bacterin ultrafiltration concentration device and an ultrafiltration concentration method, wherein the device comprises a tank body, the tank body is driven by a rotating motor to rotate around a central shaft of the tank body, an annular hollow fiber membrane column is arranged in the tank body, the tank body is divided into a first cavity and a second cavity which surrounds the first cavity by the hollow fiber membrane column, the upper surface of the tank body is correspondingly provided with a cover body, a feed inlet is arranged on the cover body and corresponds to the upper part of the second cavity, an eluent inlet is arranged on the cover body and corresponds to the upper part of the first cavity, the bottom of the first cavity is provided with a permeate outlet, and the bottom of the second cavity is provided with. The invention filters the bacteria liquid by a hollow fiber membrane column to separate macromolecular substances from small molecular substances, filters water and other small solute molecules by a centrifugal force through the hollow fiber membrane, collects the intercepted large-particle molecules to the wall and the bottom of the tank, is not easy to block the membrane, and then uses eluent to wash and filter the hollow fiber ultrafiltration membrane, thereby improving the yield and reducing the loss.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a bacterin ultrafiltration concentration device and a bacterin ultrafiltration concentration method.
Background
The importance of vaccines in disease prevention is increasing, and the quality requirements of vaccines are also increasing. In recent years, the technology of animal vaccine technology upstream tends to diversity technology, such as genetic engineering, new gene mutant strain screening and the like, and the innovation and upgrade of the downstream technology are rapidly raised. In the preparation of traditional and novel vaccines, the application of advanced separation and purification technology is an effective means for improving the efficacy of the vaccine and reducing side effects. At present, the purification and concentration methods for industrially producing the bacterial vaccine mainly comprise a continuous centrifugation method and an ultrafiltration method. The main equipment of the continuous centrifugation method is a high-speed tubular centrifuge, and the ultrafiltration method mainly uses membrane packages with different pore diameters to separate macromolecular substances from small molecular substances. The centrifugal method has the problems of low centrifugal yield, unclosed subsequent operation, complex operation, easy pollution and the like, and the ultrafiltration method has the defects that a membrane is easy to block, and the filtration effect such as yield, time and the like are influenced. Both of these two concentration methods, which operate at ambient temperature, can affect the activity of temperature sensitive antigens if they are filtered for a long time. Accordingly, there is a need for an improved technique to address this problem in the prior art.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide the bacterin ultrafiltration concentration equipment and the bacterin ultrafiltration concentration method, which solve the problems that the yield is low, a membrane package is easy to block, the concentration time is long, the activity of temperature-sensitive antigens is influenced and the like in the bacterin concentration process.
In order to achieve the purpose, the invention adopts the technical scheme that:
the utility model provides a bacterin ultrafiltration concentrator, its includes a jar body, and jar body is driven by a rotating electrical machines and is rotatory around jar center pin of body, jar internal annular hollow fiber membrane post that is equipped with, first cavity is separated into with jar body to the hollow fiber membrane post and around at the outer second cavity of first cavity, the upper surface of jar body corresponds and is equipped with the lid, it is equipped with the feed inlet to correspond second cavity top on the lid, corresponds first cavity top on the lid and is equipped with the eluant import, first cavity bottom is equipped with the permeate liquid export, and second cavity bottom is equipped with the eluant export.
Furthermore, a refrigeration jacket is arranged on the side wall of the tank body.
Furthermore, the bottom of the tank body is coaxially provided with a rotating wheel with a central shaft, and the rotating motor drives the rotating wheel to drive the tank body to rotate through a transmission belt.
Further, the feed inlet is connected with the fungus liquid storage tank of treating the concentration.
Further, feed inlet, permeate liquid export, eluant import and eluant export all are equipped with the valve, just feed inlet, eluant import, permeate liquid export, eluant export are equipped with universal joint respectively additional and are connected corresponding pipeline, and universal union can 360 rotatory make the pipeline not rotate along with the jar body.
The invention also provides a bacterin ultrafiltration concentration method using the bacterin ultrafiltration concentration equipment, which comprises the following steps:
1) installing the sterilized hollow fiber membrane column on the corresponding position of the tank body which is sterilized on line;
2) injecting water into a refrigeration jacket on the side wall of the tank body, and starting a refrigeration system;
3) connecting a discharge port of a bacterial liquid storage tank to be concentrated with a feed port of ultrafiltration concentration equipment, checking a pipeline flow path, opening a valve of the feed port, passing through a liquid outlet valve, closing all other valves, and allowing the bacterial liquid to be concentrated to enter a second cavity through the feed port;
4) the power supply is turned on for filtration, the tank body is driven by the rotating motor to rotate around the central shaft of the tank body so as to generate centrifugal force, the centrifugal speed is slowly adjusted to enable the centrifugal speed to reach a required value, the centrifugal force applied to the micromolecules in the second cavity is small, the micromolecules penetrate through the hollow fiber membrane column to enter the first cavity and flow out from the permeate liquid outlet at the bottom of the first cavity, the macromolecules in the second cavity are gathered to the tank wall and the tank body, the hollow fiber membrane column is not easy to block,
5) after the bacteria liquid is completely filtered, closing the feed inlet valve and the permeate outlet valve, opening the eluent outlet valve, and collecting the concentrated solution;
6) the bacterins entering the hollow fiber membrane column are eluted to improve the yield: opening an eluent inlet valve, injecting eluent into the first cavity, adjusting the centrifugal speed to reach a required value, eluting bacteria entering the membrane, collecting the eluent through an eluent outlet valve, merging the eluent into a concentrated solution, and finishing the concentration of the bacterial liquid;
7) and after the concentration is finished, closing the switch, and after the tank body stops rotating, detaching the hollow fiber membrane column and cleaning.
By adopting the technical scheme, the invention has the advantages that:
1. the invention filters the bacteria liquid by a hollow fiber membrane column to separate macromolecular substances from small molecular substances, and simultaneously filters water and other small solute molecules by a simple material method such as centrifugal force and the like through the hollow fiber membrane, and gathers the intercepted large particle molecules to the tank wall and the tank bottom, thereby being not easy to block the membrane and reducing the filtering time.
2. The refrigeration jacket is arranged on the wall of the tank, cold water can be injected into the side wall of the tank, and the antigen is prevented from influencing the activity due to temperature rise and overlong time during filtration.
3. The invention uses the eluent to wash and filter the hollow fiber ultrafiltration membrane, combines the washed and filtered eluent into the concentrated solution, collects most of the total thallus antigen solution, improves the yield and reduces the loss.
4. The invention can set different concentration parameters according to different bacterin types, including centrifugal rotation speed, hollow fiber membrane aperture and the like. The concentrated solution can be collected according to different requirements of products, and the permeate can also be collected.
5. The method has the characteristics of simple equipment, no complex operation process, high separation speed, high efficiency, energy conservation, high working efficiency, high recovery rate, high product titer, suitability for industrial production, capability of realizing totally-enclosed operation and reducing pollution risk and the like.
Drawings
The invention is described in further detail below with reference to the accompanying drawings and the detailed description;
FIG. 1 is a schematic structural diagram of a bacterin ultrafiltration concentration device of the present invention.
Detailed Description
As shown in figure 1, the invention discloses a bacterin ultrafiltration concentration device, which comprises a tank body 1, wherein the tank body 1 is driven by a rotary motor 2 to rotate around the central shaft of the tank body 1, an annular hollow fiber membrane column 3 is arranged in the tank body 1, the tank body 1 is divided into a first cavity 11 and a second cavity 12 surrounding the first cavity 1 by the hollow fiber membrane column 3, a cover body 4 is correspondingly arranged on the upper surface of the tank body 1, a feed inlet 5 is arranged on the cover body 4 corresponding to the upper part of the second cavity 12, an eluent inlet 6 is arranged on the cover body 4 corresponding to the upper part of the first cavity 11, the bottom of the first cavity 11 is provided with a permeate outlet 7, the bottom of the second cavity 12 is provided with an eluent outlet 8, wherein the feed inlet 5, eluent import 6, permeate liquid export 7, the pipeline of eluent export 8 install universal joint additional respectively, can 360 rotations for the pipeline does not rotate along with the jar body.
Furthermore, a refrigeration jacket is arranged on the side wall of the tank body 1.
Further, the bottom of the tank body 1 is coaxially provided with a rotating wheel 9 along a central axis, and the rotating motor 2 drives the rotating wheel 9 to drive the tank body 1 to rotate through a transmission belt.
Further, the feed inlet 5 is connected with a bacterium liquid storage tank to be concentrated.
Furthermore, the feed inlet 5, the permeate outlet 7, the eluent inlet 6 and the eluent outlet 8 are all provided with valves.
The bacterin ultrafiltration concentration method applying the bacterin ultrafiltration concentration equipment comprises the following steps:
1) installing the sterilized hollow fiber membrane column on the corresponding position of the tank body;
2) injecting water into a refrigeration jacket on the side wall of the tank body, and starting a refrigeration system;
3) connecting a discharge port of a bacterial liquid storage tank to be concentrated with a feed port of ultrafiltration concentration equipment, checking a pipeline flow path, opening a valve of the feed port, passing through a liquid outlet valve, closing all other valves, and allowing the bacterial liquid to be concentrated to enter a second cavity through the feed port;
4) the power supply is turned on for filtration, the tank body is driven by the rotating motor to rotate around the central shaft of the tank body so as to generate centrifugal force, the centrifugal speed is slowly adjusted to enable the centrifugal speed to reach a required value, the centrifugal force applied to the micromolecules in the second cavity is small, the micromolecules penetrate through the hollow fiber membrane column to enter the first cavity and flow out from the permeate liquid outlet at the bottom of the first cavity, the macromolecules in the second cavity are gathered to the tank wall and the tank body, the hollow fiber membrane column is not easy to block,
5) after the bacteria liquid is completely filtered, closing the feed inlet valve and the permeate outlet valve, opening the eluent outlet valve, and collecting the concentrated solution;
6) the bacterins entering the hollow fiber membrane column are eluted to improve the yield: opening an eluent inlet valve, injecting eluent into the first cavity, adjusting the centrifugal speed to reach a required value, eluting bacteria entering the membrane, collecting the eluent through an eluent outlet valve, merging the eluent into a concentrated solution, and finishing the concentration of the bacterial liquid;
7) and after the concentration is finished, closing the switch, and after the tank body stops rotating, detaching the hollow fiber membrane column and cleaning.
Example 1
And (3) concentrating the inactivated vaccine of the haemophilus parasuis: 30 ml of haemophilus parasuis bacterial liquid is prepared, centrifugal ultrafiltration is carried out on the bacterial liquid by adopting a hollow fiber membrane column with the aperture of 300KD, a permeate is removed, a concentrated solution is collected, and loss is reduced. The method comprises the following specific steps:
1) installing the sterilized hollow fiber membrane column on the corresponding position of the tank body;
2) injecting water into a refrigeration jacket on the side wall of the tank body, and starting a refrigeration system;
3) connecting a discharge port of a haemophilus parasuis liquid storage tank to be concentrated with a feed port of ultrafiltration concentration equipment, checking a pipeline flow path, opening a valve of the feed port, passing through a liquid outlet valve, closing all other valves, and allowing the liquid to be concentrated to enter a second cavity through the feed port;
4) the power supply is turned on for filtration, the tank body is driven by the rotating motor to rotate around the central shaft of the tank body so as to generate centrifugal force, the centrifugal speed is slowly adjusted to 5000r/min, the centrifugal force applied to the micromolecules in the second cavity is small, the micromolecules enter the first cavity through the hollow fiber membrane column and flow out from the permeate liquid outlet at the bottom of the first cavity, the macromolecules in the second cavity are gathered to the tank wall and the tank body, the hollow fiber membrane column is not easy to block,
5) after the bacteria liquid is completely filtered, closing the feed inlet valve and the permeate outlet valve, opening the eluent outlet valve, and collecting the concentrated solution;
6) the bacterins entering the hollow fiber membrane column are eluted to improve the yield: opening an eluent inlet valve, injecting eluent into the first cavity, adjusting the centrifugal speed to 5000r/min, eluting bacteria entering a membrane, collecting eluent through an eluent outlet valve, merging the eluent into a concentrated solution, and finishing the concentration of the bacterial liquid;
7) and after the concentration is finished, closing the switch, and after the tank body stops rotating, detaching the hollow fiber membrane column and cleaning.
Compared with a column type centrifuge filtering system, the device for concentrating the inactivated vaccine of the haemophilus parasuis has the advantages that 30 ten thousand ml of antigen is concentrated, the filtering time is shortened to 110min from the original average 200min, and the yield is improved to 80% from the original 60%.
Example 2
Concentration of mycoplasma hyopneumoniae inactivated vaccine: 30 thousands ml of mycoplasma hyopneumoniae fermentation liquor is prepared, a hollow fiber membrane column with the aperture of 750KD is adopted, the bacterial liquid is subjected to centrifugal ultrafiltration, the permeate is removed, the concentrated solution is collected, and the loss is reduced. The method comprises the following specific steps:
1) installing the sterilized hollow fiber membrane column on the corresponding position of the tank body;
2) injecting water into a refrigeration jacket on the side wall of the tank body, and starting a refrigeration system;
3) connecting a discharge port of a mycoplasma hyopneumoniae fermentation liquid storage tank to be concentrated with a feed port of ultrafiltration concentration equipment, checking a pipeline flow path, opening a valve of the feed port, closing a permeate outlet valve, closing all other valves, and allowing a bacterial liquid to be concentrated to enter a second cavity through the feed port;
4) the power supply is turned on for filtration, the tank body is driven by the rotating motor to rotate around the central shaft of the tank body so as to generate centrifugal force, the centrifugal speed is slowly adjusted to 3000r/min, the centrifugal force applied to the micromolecules in the second cavity is small, the micromolecules enter the first cavity through the hollow fiber membrane column and flow out from the permeate liquid outlet at the bottom of the first cavity, the macromolecules in the second cavity are gathered to the tank wall and the tank body, the hollow fiber membrane column is not easy to block,
5) after the bacteria liquid is completely filtered, closing the feed inlet valve and the permeate outlet valve, opening the eluent outlet valve, and collecting the concentrated solution;
6) the bacterins entering the hollow fiber membrane column are eluted to improve the yield: opening an eluent inlet valve, injecting eluent into the first cavity, adjusting the centrifugal speed to 3000r/min, eluting bacteria entering the membrane, collecting eluent through an eluent outlet valve, merging the eluent into concentrated solution, and finishing the concentration of the bacterial solution;
7) and after the concentration is finished, closing the switch, and after the tank body stops rotating, detaching the hollow fiber membrane column and cleaning.
Compared with a column type centrifuge filtering system, the device for concentrating the inactivated vaccine of the haemophilus parasuis has the advantages that 30 ten thousand ml of antigen is concentrated, the filtering time is shortened to 100min from the original average 160min, and the yield is improved to 80% from the original 60%.
Claims (8)
1. The utility model provides a bacterin ultrafiltration enrichment facility which characterized in that: it is including a jar body, and jar body is rotatory around jar center pin of body by a rotating electrical machines drive, jar internal annular hollow fiber membrane post that is equipped with, first cavity is separated into with jar body to hollow fiber membrane post and around the second cavity outside first cavity, the upper surface of jar body corresponds and is equipped with the lid, it is equipped with the feed inlet to correspond second cavity top on the lid, corresponds first cavity top on the lid and is equipped with the eluant import, first cavity bottom is equipped with the permeate liquid export, and second cavity bottom is equipped with the eluant export.
2. The bacterin ultrafiltration concentration device of claim 1, wherein: and a refrigeration jacket is arranged on the side wall of the tank body.
3. The bacterin ultrafiltration concentration device of claim 1, wherein: the bottom of the tank body is coaxially provided with a rotating wheel with a central shaft, and the rotating motor drives the rotating wheel to drive the tank body to rotate through a transmission belt.
4. The bacterin ultrafiltration concentration device of claim 1, wherein: the feed inlet is connected with the fungus liquid storage tank to be concentrated.
5. The bacterin ultrafiltration concentration device of claim 1, wherein: the feed inlet, the permeate outlet, the eluent inlet and the eluent outlet are all provided with valves.
6. A bacterin ultrafiltration concentration method using the bacterin ultrafiltration concentration apparatus of any one of claims 1 to 5, wherein: which comprises the following steps:
installing the sterilized hollow fiber membrane column on the corresponding position of the sterilized tank body;
2) injecting water into a refrigeration jacket on the side wall of the tank body, and starting a refrigeration system;
3) connecting a discharge port of a bacterial liquid storage tank to be concentrated with a feed port of ultrafiltration concentration equipment, checking a pipeline flow path, opening a valve of the feed port, passing through a liquid outlet valve, closing all other valves, and allowing the bacterial liquid to be concentrated to enter a second cavity through the feed port;
4) the power supply is turned on for filtration, the tank body is driven by the rotating motor to rotate around the central shaft of the tank body so as to generate centrifugal force, the centrifugal speed is slowly adjusted to reach a required value, the centrifugal force applied to the micromolecules in the second cavity is small, the micromolecules penetrate through the hollow fiber membrane column, enter the first cavity and flow out from a liquid penetrating outlet at the bottom of the first cavity, and the macromolecules in the second cavity are gathered to the tank wall and the tank body, so that the hollow fiber membrane column is not easy to block;
5) after the bacteria liquid is completely filtered, closing the feed inlet valve and the permeate outlet valve, opening the eluent outlet valve, and collecting the concentrated solution;
6) the bacterins entering the hollow fiber membrane column are eluted to improve the yield: opening the eluent inlet valve, injecting eluent into the first cavity, adjusting the centrifugal speed to reach a required value, eluting bacteria entering the membrane, collecting the eluent through the eluent outlet valve, merging the eluent into the concentrated solution, and finishing the concentration of the bacteria liquid.
7. The method for ultrafiltration concentration of a bacterin according to claim 6, wherein: it also includes the following steps: 7) and after the concentration is finished, closing the switch, and after the tank body stops rotating, detaching the hollow fiber membrane column and cleaning.
8. The method for ultrafiltration concentration of a bacterin according to claim 6, wherein: feed inlet, eluent import, permeate liquid export, eluent export are equipped with universal joint respectively additional and are connected corresponding pipeline, and universal movable joint can 360 rotatory make the pipeline not rotate along with the jar body.
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| CN109406252A (en) * | 2018-09-28 | 2019-03-01 | 苏州大学 | A kind of centrifugal ultrafiltration sample processing device and its application method |
| CN110382092A (en) * | 2017-03-09 | 2019-10-25 | 奥加诺株式会社 | It evaluates the method for the cleannes level of hollow fiber membrane device, wash the method for hollow fiber membrane device and the wash mill for hollow fiber membrane device |
| CN210631942U (en) * | 2019-06-06 | 2020-05-29 | 李宇宙 | Novel centrifugal multi-gradient membrane separation device |
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2020
- 2020-10-28 CN CN202011173182.4A patent/CN112316727A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101898088A (en) * | 2009-05-25 | 2010-12-01 | 河北医科大学 | Centrifugal ultrafiltration device suitable to be used in preprocessing of micro sample |
| CN101973602A (en) * | 2010-10-08 | 2011-02-16 | 褚礼政 | Centrifugal sewage treater |
| WO2012108754A2 (en) * | 2011-02-08 | 2012-08-16 | Universidad Autónoma De Yucatán | Method for the desalination of water by means of reverse osmosis, using a centrifugal filter and involving the generation of dean vortices |
| CN110382092A (en) * | 2017-03-09 | 2019-10-25 | 奥加诺株式会社 | It evaluates the method for the cleannes level of hollow fiber membrane device, wash the method for hollow fiber membrane device and the wash mill for hollow fiber membrane device |
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