CN111903666A - Hu sheep semen freezing tube and thawing apparatus - Google Patents

Abstract

The invention discloses a Hu sheep semen freezing pipe which comprises a pipe body, a pipe cover matched with an opening at the top of the pipe body and an accommodating cavity arranged in the pipe body, wherein a clamping convex part is arranged on the pipe cover, the pipe body comprises an outer pipe and an inner pipe, annular accommodating cavities are formed between the outer pipe and the inner pipe at intervals, a cavity is arranged on the inner pipe, and the lower end of the cavity is communicated with the outside. Compared with the traditional method that liquid nitrogen only contacts with the outer wall of the tank body, the method increases the contact area between the pipe body and the liquid nitrogen and the contact area between the inner wall of the pipe body and semen in the freezing process, accelerates the freezing speed of the semen, reduces the damage of freezing to the semen and improves the survival rate of the semen.

Description

A kind of Hu sheep semen freezing tube and thawing device

technical field

The invention relates to the technical field of Hu sheep breeding, in particular to a Hu sheep semen freezing tube and a thawing device.

Background technique

Hu sheep is one of the important livestock in the Taihu Plain, and it is a first-class protected local livestock and poultry breed in my country. Hu sheep are medium in physique, both male and female have no horns, long and narrow head, raised nose bridge, large drooping ears, slender neck, long and narrow body, straight back and waist, slightly drooping abdomen, flat tail, upturned tail tip, and slanted limbs. Thin and tall. The coat is all white, the belly hair is thick, thin and short, and the physique is strong. Hu sheep is a rare breed of white lambskin sheep. It has early maturity, four seasons of estrus, two litters a year, multiple lambs per litter, good lactation performance, rapid growth and development, ideal meat production performance after improvement, high temperature and humidity resistance and other excellent traits. Distribution In the Taihu Lake area of my country, the Chinese lambskin sheep breeds are kept year-round, and the Xiaohu sheepskins that are slaughtered and peeled 1 to 2 days after giving birth are famous for their beautiful patterns.

Semen cryopreservation and artificial insemination technology play a pivotal role in modern animal husbandry production. The use of frozen semen for artificial insemination is not limited by time, region, breed and individual, and greatly reduces the number of male animals and production costs. Improve the utilization rate of high-quality male animals and improve the economic benefits of the breeding industry. At the same time, the technology is of great significance for variety introduction, hybrid improvement, improvement of reproductive performance, disease control and protection of genetic diversity. Sheep sperm are more fragile than chicken and bovine sperm, and are easily damaged during freezing and thawing.

SUMMARY OF THE INVENTION

The purpose of the present invention is to address the above-mentioned problems in the prior art, and to provide a Hu sheep semen freezing tube and a thawing device that are helpful for reducing the damage to sperm.

The purpose of the present invention can be achieved by the following technical solutions: a frozen semen tube of Hu sheep, comprising a tube body, a tube cover matched with the opening at the top of the tube body, and a accommodating cavity provided in the tube body, and the tube cover is provided with There are clamping protrusions, the tube body includes an outer tube and an inner tube, an annular accommodating cavity is formed at intervals between the outer tube and the inner tube, the inner tube is provided with a cavity, and the lower end of the cavity is connected to the inner tube. Connected to the outside world.

During the freezing process of the present invention, the liquid nitrogen enters the cavity and contacts the outer wall of the inner tube. Compared with the traditional liquid nitrogen that only contacts the outer wall of the tank, the present invention increases the contact area between the tube body and the liquid nitrogen during the freezing process. The contact area between the inner wall of the tube and the semen accelerates the freezing speed of the semen, reduces the damage to sperm caused by freezing, and improves the survival rate of the sperm; and the freezing tube in the present invention is in the process of transportation, because the semen is in the annular accommodating cavity. , the semen is not easy to shake violently with the shaking of the vehicle before, once the semen shakes violently, it is easy to damage the sperm and affect its survival rate; after the semen is stored in this freezing tube, since the semen is in the annular container, the semen will be exhausted. It may be attached to the wall of the tube body in a state of evenly spreading along the tube wall, which is convenient for operators to observe whether there are impurities in the semen.

As a preferred solution of the present invention, a plurality of connecting pipes are connected between the outer pipe and the inner pipe, the connecting pipe is provided with a flow channel, and the outer pipe is provided with a first opening communicating with one end of the flow channel, so The inner tube is provided with a second opening that communicates with the other end of the flow channel; when the freezing tube is placed in liquid nitrogen for freezing, the liquid nitrogen will flow into the flow channel to contact the inner wall of the connecting tube, and the outer wall of the connecting tube will be in contact with the tube. The semen in the body is in contact, and the contact area between the semen and liquid nitrogen is further increased, which speeds up the freezing speed of semen, reduces the damage to sperm caused by freezing, and improves the survival rate of sperm. The water will flow into the flow channel and come into contact with the inner wall of the connecting pipe, thereby improving the thawing efficiency of the semen; and the arrangement of multiple connecting pipes improves the overall strength of the pipe body, which is not easy to be damaged during transportation, which is convenient for the preservation and transportation of semen .

As a preferred solution of the present invention, the cross-section of the connecting tube is an inverted triangle, and the shape of the inverted triangle is such that when the operator thaws the semen and dumps it outwards, the semen in the holding cavity will be blocked by the sharp corners of the connecting tube when the semen flows downward. It can effectively prevent the semen from adhering to the connecting tube when it flows, which will reduce the utilization rate of the semen, so that the semen in the accommodating cavity can be discharged as much as possible to improve the utilization rate.

As a preferred solution of the present invention, the inner tube is provided with a plurality of first arc-shaped portions along its circumferential direction; the outer tube is provided with a plurality of second arc-shaped portions along its circumferential direction; the first arc-shaped portion And the setting of the second arc-shaped part further increases the contact area between the semen in the holding chamber and the liquid nitrogen or hot water, which helps to improve the freezing and thawing efficiency of the semen. It is not easy to roll when placed on the test bench, the placement is more stable, and the risk of damage to the tube body is reduced.

As a preferred solution of the present invention, the clamping protrusions are provided with two clamping grooves, the two clamping grooves are respectively symmetrically arranged on both sides of the clamping protrusions, and the clamping grooves are inclined upward from the outside to the inside. Setting; During the long-term operation of vitrifying and thawing embryos/gametes, the front end of the forceps will freeze due to repeated in and out of liquid nitrogen. Through the setting of the clamping grooves on both sides, when the tweezers clamp the clamping protrusions, both sides of the tweezers can be respectively clamped into the corresponding clamping grooves, so as to exert a certain force to lift the cryotube, which improves the tweezers clamping and freezing. tube stability.

A thawing device for a freezing tube of Hu sheep semen, comprising a base and a thawing part arranged on the base, the thawing part is provided with a plurality of thawing grooves for the tube body to be inserted into, and the thawing groove is provided with a thawing groove for inserting into the empty space. The thawing rod in the cavity is provided with a heating driver for heating the thawing part and the thawing tank; when the semen in the freezing tube needs to be thawed, the freezing tube is inserted into the thawing tank, and the thawing rod is inserted into the freezing tube at the same time. In the cavity, the freezing rod and the inner wall of the thawing tank can limit the position of the freezing tube, so that the thawing device can be used synchronously during transportation, so as to adapt to some situations that need to be used in other places after thawing. Simultaneous thawing during transportation greatly saves the preparation time in the early stage, and timely utilization of semen after thawing is helpful to improve the survival rate of semen.

As a preferred solution of the present invention, the thawing part has a first thawing cavity, the thawing rod has a second thawing cavity, the first thawing cavity is communicated with the second thawing cavity, and the first thawing cavity and the second thawing cavity are communicated with each other. Both the two thawing chambers are filled with thawing liquid; the present invention heats the thawing liquid by heating the driver, and then transfers the heat to the freezing tube through the thawing liquid to thaw the semen, compared with directly transferring the heat of the heating driving member to the freezing tube. , the thawing liquid can absorb the heat of the heating driver and evenly transfer it to the freezing tube to thaw the semen. The temperature variation range of the frozen liquid is small, which is convenient to control the temperature within a certain range, and can avoid the semen thawing caused by the temperature is too low Incomplete and overheated semen inactivation occurs

As a preferred solution of the present invention, the thawing part and the thawing rod are made of silica gel material; the silica gel material makes both the thawing part and the thawing rod have good thermal conductivity, which is convenient for fast and efficient heat transfer of the thawing liquid to the freezing tube, At the same time, the silicone material has good deformation ability. When the freezing tube is inserted into the thawing tank, the thawing part will deform to wrap the freezing tube and improve the heat conduction ability. The degree of fit, thereby improving the thermal conductivity of the thawing rod; and the thawing part made of silicone material has a very good buffering capacity when it is filled with thawing liquid. Most of the vibration shock received by the cryotube will be buffered by the thawing part, so that the cryotube can be thawed in a relatively stable condition, ensuring the survival rate of the semen after reaching the destination, and ensuring that the semen can be used in time.

As a preferred solution of the present invention, the volume of the accommodating cavity is 5-15ml; if the volume is too small, the space utilization rate of the cryotube will be too low, which will greatly increase the cost. It is easy to shake in the cryovial and is more likely to fall when the operator picks it up with tweezers.

Compared with the prior art, compared with the traditional liquid nitrogen in the present invention, it only contacts the outer wall of the tank. It improves the freezing speed of semen, reduces the damage to sperm caused by freezing, and improves the survival rate of sperm.

Description of drawings

1 is a schematic structural diagram of Embodiment 1 of the present invention;

2 is a cross-sectional view one of the first embodiment of the present invention;

Fig. 3 is the partial schematic diagram of A place in Fig. 2;

Fig. 4 is a partial schematic diagram at B in Fig. 2;

5 is a sectional view 2 of Embodiment 1 of the present invention;

6 is a schematic structural diagram of Embodiment 2 of the present invention;

7 is a cross-sectional view of the second embodiment of the present invention;

In the figure, 1, the tube body; 2, the tube cover; 3, the accommodating cavity; 4, the clamping protrusion; 5, the outer tube; 6, the inner tube; 7, the accommodating cavity; 8, the cavity; 9, the connecting tube; 10, runner; 11, first opening; 12, second opening; 13, first arc part; 14, second arc part; 15, clamping groove; 16, base; 17, defrosting part; 18, 19. thawing rod; 20. heating driver; 21. first thawing cavity; 22. second thawing cavity.

Detailed ways

The following are specific embodiments of the present invention and the accompanying drawings to further describe the technical solutions of the present invention, but the present invention is not limited to these embodiments.

Example 1

As shown in Figures 1-5, a Hu sheep semen freezing tube and thawing device, comprising a tube body 1, a tube cover 2 matched with the opening at the top of the tube body 1, and an accommodating cavity 3 disposed in the tube body 1, the tube The body 1 is provided with an opening for filling semen, the tube cover 2 is mounted on the opening of the tube body 1 through a thread to seal the accommodating cavity 3, the tube cover 2 is provided with a clamping protrusion 4, and the clamping protrusion 4 is on the tube cover 2. The end face is vertically upwardly protruding cylindrical body, the clamping protrusion 4 is integrally formed with the tube cover 2, the tube body 1 includes an outer tube 5 and an inner tube 6, the outer tube 5 and the inner tube 6 are both cylindrical, and the inner tube 6 is on the A plurality of first arc-shaped portions 13 are arranged along its circumferential direction, and a plurality of second arc-shaped portions 14 are arranged on the outer tube 5 along its circumferential direction. An annular accommodating cavity 7 is formed at intervals between the tubes 6, the upper end of the inner tube 6 is provided with a cover, the lower part of the cover communicates with the outside to form a cavity 8, and the lower end of the inner tube 6 and the lower end of the outer tube 5 are sealed and connected through the bottom cover , to prevent the leakage of semen in the accommodating cavity 7 .

Specifically, a plurality of connecting pipes 9 are connected between the outer pipe 5 and the inner pipe 6 , and a plurality of connecting pipes 9 are arranged at equal intervals along the height direction of the pipe body 1 , and are arranged at equal intervals along the circumferential direction of the pipe body 1 . 4 groups, the connecting pipe 9 is arranged horizontally between the outer pipe 5 and the inner pipe 6, the connecting pipe 9 is provided with a flow channel 10, the flow channel 10 extends along the length direction of the connecting pipe 9, and the outer pipe 5 is provided with a flow channel. 10 A first opening 11 communicated at one end, the inner tube 6 is provided with a second opening 12 communicated with the other end of the flow channel 11, the flow channel 10 communicates with the outer space of the outer tube 5 through the first opening 11, and the flow channel 10 It communicates with the cavity 8 through the second opening 12,

In the present embodiment, the cross-section of the connecting pipe 9 is an inverted triangle. When the inverted pipe body 1 pours the semen, the sharp corner of the connecting pipe 9 can divide the semen to prevent the semen from adhering to the connecting pipe 9 and improve the semen. utilization rate.

In this embodiment, in order to prevent the operator from slipping off when the operator uses tweezers to grasp the clamping protrusions 4, the clamping protrusions 4 are provided with two clamping grooves 15, and the two clamping grooves 15 are respectively symmetrically arranged in the clamping protrusions 4. On both sides of the convex portion 4 , the clamping grooves 15 are inclined upward from the outside to the inside.

Embodiment 2

As shown in Figures 6-7, a thawing device for Hu sheep semen freezing tube includes a base 16 and a thawing part 17 arranged on the base 16, the base 16 is arranged in a square shape, and the thawing part 17 is fixed on the upper end surface of the base 16 The thawing part 17 is provided with a plurality of thawing grooves 18 for inserting the pipe body 1. The thawing grooves 18 are formed by the downward depression of the upper end of the thawing part 17, and the groove diameter is the same as the diameter of the outer pipe 5. The thawing grooves 18 There is a thawing rod 19 for inserting into the cavity 8. The thawing rod 19 is formed by the upward protrusion of the bottom wall of the thawing groove 18. The thawing part 17 has a first thawing cavity 21, and the thawing rod 19 has a second thawing cavity. 22. The first thawing cavity 21 and the second thawing cavity 22 are communicated with each other. The first thawing cavity 21 and the second thawing cavity 22 are both filled with thawing liquid. The base 16 is provided with a heating driver 20 for heating the thawing liquid. The driving member 20 is a heating rod that can be purchased directly from the market, and the thawing part 17 and the thawing rod 19 are made of silica gel material.

The specific embodiments described herein are merely illustrative of the spirit of the invention. Those skilled in the art to which the present invention pertains can make various modifications or additions to the described specific embodiments or substitute in similar manners, but will not deviate from the spirit of the present invention or go beyond the definitions of the appended claims range.

Although the term is used extensively in this paper, the possibility of using other terms is not excluded. These terms are used only to more conveniently describe and explain the essence of the present invention; it is contrary to the spirit of the present invention to interpret them as any kind of additional limitation.

Claims (9)

1. The utility model provides a hu sheep seminal fluid cryostraw which characterized in that: including body (1), with body (1) top opening part matched with tube cap (2) and locate body (1) in hold chamber (3), be equipped with centre gripping convex part (4) on tube cap (2), body (1) includes outer tube (5) and inner tube (6), the interval forms annular chamber (7) that holds between outer tube (5) and inner tube (6), be equipped with cavity (8) on inner tube (6), cavity (8) lower extreme is linked together with the external world.

2. The hu-sheep semen freezing pipe as claimed in claim 1, wherein: be connected with a plurality of connecting pipes (9) between outer tube (5) and inner tube (6), be equipped with runner (10) in connecting pipe (9), be equipped with first opening (11) that are linked together with runner (10) one end on outer tube (5), be equipped with second opening (12) that are linked together with the runner (11) other end on inner tube (6).

3. The hu-sheep semen freezing pipe as claimed in claim 2, wherein: the cross section of the connecting pipe (9) is in an inverted triangle shape.

4. The hu-sheep semen freezing pipe as claimed in claim 1, wherein: a plurality of first arc-shaped parts (13) are arranged on the inner pipe (6) along the circumferential direction of the inner pipe; a plurality of second arc-shaped parts (14) are arranged on the outer pipe (5) along the circumferential direction of the outer pipe.

5. The hu-sheep semen freezing pipe as claimed in claim 1, wherein: be equipped with two centre gripping grooves (15) on centre gripping convex part (4), two centre gripping groove (15) symmetry respectively locates centre gripping convex part (4) both sides, and centre gripping groove (15) are outside-in slant slope and incline upward and set up.

6. The thawing device of the Hu sheep semen freezing pipe as claimed in claim 1, wherein: including base (16) and locate thawing portion (17) on base (16), be equipped with a plurality of confession bodys (1) male thawing groove (18) on thawing portion (17), be equipped with in thawing groove (18) and be used for inserting stick (19) that unfreeze to in cavity (8), be equipped with on base (16) and be used for heating driving piece (20) to thawing portion (17) and thawing groove (18) heating.

7. The thawing device of the Hu sheep semen freezing pipe as claimed in claim 1, wherein: the thawing device is characterized in that a first thawing cavity (21) is formed in the thawing part (17), a second thawing cavity (22) is formed in the thawing rod (19), the first thawing cavity (21) is communicated with the second thawing cavity (22), and thawing liquid is filled in the first thawing cavity (21) and the second thawing cavity (22).

8. The thawing device of the Hu sheep semen freezing pipe as claimed in claim 7, wherein: the unfreezing part (17) and the unfreezing rod (19) are made of silica gel materials.

9. The thawing device of the Hu sheep semen freezing pipe as claimed in claim 1, wherein: the volume of the accommodating cavity (7) is 5-15 ml.

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