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CN111821235A - Photo-protection plant extraction composition and application thereof - Google Patents

Photo-protection plant extraction composition and application thereof Download PDF

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CN111821235A
CN111821235A CN202010669767.9A CN202010669767A CN111821235A CN 111821235 A CN111821235 A CN 111821235A CN 202010669767 A CN202010669767 A CN 202010669767A CN 111821235 A CN111821235 A CN 111821235A
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extract
parts
photoprotective
composition
plant
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招敏聪
孙美娟
杨悬
郭文姣
贺锐
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Plant Doctor Guangdong Biotechnology Co ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/004Aftersun preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/005Preparations for sensitive skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59Mixtures
    • A61K2800/592Mixtures of compounds complementing their respective functions
    • A61K2800/5922At least two compounds being classified in the same subclass of A61K8/18
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/74Biological properties of particular ingredients
    • A61K2800/78Enzyme modulators, e.g. Enzyme agonists
    • A61K2800/782Enzyme inhibitors; Enzyme antagonists

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  • Gerontology & Geriatric Medicine (AREA)
  • Cosmetics (AREA)

Abstract

The invention discloses a photoprotective plant extract composition and application thereof. The photoprotective plant extract composition comprises the following components: apple extract, butterflybush flower extract, rhodiola extract, and cistanche extract. The photo-protection plant extract composition effectively absorbs ultraviolet light and blue light through the synergistic effect of the components, has excellent anti-ultraviolet and anti-blue light effects, can effectively inhibit the increase of active oxygen caused by the induction of ultraviolet light, blue light and red light, keeps the activity of human epidermal keratinocytes and the activity of human dermal cells, promotes the generation of collagen, effectively resists the generation of free radicals, can achieve the comprehensive photo-protection effect, is beneficial to repairing the damage of radiation to the skin and relieves the skin sensitivity problem.

Description

Photo-protection plant extraction composition and application thereof
Technical Field
The invention relates to the technical field of radiation protection, and particularly relates to a light-protection plant extraction composition and application thereof.
Background
Ultraviolet rays in sunlight damage human skin to a certain extent, and the epidermis of a person can be tanned by light irradiation of 280 nm-400 nm. Among them, light of 280nm to 320nm, called UV-B light, causes skin burn and erythema to destroy the formation of natural suntan, and thus UV-B irradiation should be masked off. Also, UV-A light of 320nm to 400nm wavelength, which causes tanning, is itself prone to adverse effects, especially in the case of sensitive skin or skin which is constantly exposed to sun radiation, especially in the case of skin which loses elasticity and wrinkles, leading to premature ageing, and it is therefore necessary to mask UV-A radiation from the skin. At present, in daily life of people, besides ultraviolet rays, blue light produced by electronic products cannot be looked at a glance, and with the popularization of electronic products such as mobile phones, computers, televisions, readers and LED lamps, blue light can be generated, so that the risk that people suffer from blue light injury is increased. The blue light is used as one of visible light, the wavelength of the blue light is as long as 400-500nm, and the damage to the skin is obvious. As the wave band with the shortest wavelength and the highest energy in the visible spectrum, the blue light has stronger penetrating power than ultraviolet rays, so the blue light is easier to penetrate into the hypodermis of the skin, a large amount of active oxygen is generated under the radiation of the blue light, the excessive active oxygen can degrade collagen, the synthesis of matrix metalloproteinase MMP is increased, the skin aging process is accelerated, oxidative damage is caused, the skin is aged, and the skin is seriously damaged. Just as with uv protection, it is also necessary to protect the skin from the hazards of blue light, which can help alleviate premature aging of the skin. In addition, the damage of infrared rays to the skin is not paid enough attention, the penetrating power of heat radiation caused by the infrared rays to the skin is stronger than that of ultraviolet rays, the damage of the ultraviolet rays can be enhanced, and when the skin is irradiated by the ultraviolet rays and the infrared rays, erythema is more easily generated, and the moisture in the skin is correspondingly lost. In addition, as the climate becomes warm and the ozone layer is destroyed, the exposure opportunities of visible blue light (400-.
Based on the above, a few cosmetics with ultraviolet resistance, blue light resistance and infrared resistance appear in the market, but the cosmetics generally have the defects of unobvious efficacy, easy allergy phenomenon, no universality and the like. In the technical specification of cosmetic safety (2015 edition), 27 quasi-use sunscreens are regulated, wherein 25 organic sunscreens and 2 inorganic sunscreens are provided, but the organic sunscreens and the inorganic sunscreens are artificially synthesized chemical sunscreen structures, and the ingredients can only protect ultraviolet rays in a UVB wave band and a UVA wave band generally, do not relate to the protection of a near infrared wave band and a visible blue wave band, and the research on plant compositions capable of effectively preventing ultraviolet rays, blue light and infrared rays is less. CN109771353A discloses a blue light and infrared resistant composition and cosmetics thereof, which comprises some plant extracts, which have certain blue light and infrared resistant effects, but do not give related technical suggestions on free radical scavenging and collagen generating effects, and cannot provide comprehensive blue light and infrared resistant photoprotective effects for sensitive skin.
Disclosure of Invention
The invention aims to solve the technical problems that the existing anti-radiation preparation cannot realize full-light protection, is particularly not suitable for sensitive skin, and has defects in the aspects of free radical removal, collagen generation and the like, and provides a photoprotective plant extract composition which can well resist blue light, prevent infrared and repair photodamaged skin through the synergistic effect of various components.
Another object of the present invention is to provide the use of a photoprotective plant extract composition for the preparation of UV-, blue-and IR-resistant photoprotective preparations.
It is still another object of the present invention to provide a use of a photoprotective plant extract composition for preparing a sunscreen preparation having the effects of reducing the generation of reactive oxygen species and scavenging free radical DPPH.
The invention also aims to provide application of the photoprotective plant extract composition in preparing a sunscreen preparation with the effects of inhibiting matrix metalloproteinase MMP degradation and promoting collagen production.
It is a further object of the present invention to provide a sunscreen formulation.
The above purpose of the invention is realized by the following technical scheme:
a photoprotective plant extract composition comprising the following components: apple extract, butterflybush flower extract, rhodiola extract, and cistanche extract.
The components of the photoprotective plant extract composition of the invention are illustrated below:
the fructus Mali Pumilae extract is also called yellow Melia, and is dried trunk of yellow Melia (Hespeerhusacrenulata (Roxb.) Roem) belonging to Citrus of Rutaceae, whose bark contains polyphenol, flavanone, alkaloid, coumarin, lignan, sterols and triterpenes, etc., and has antiinflammatory, antibacterial, mosquito preventing, ultraviolet injury preventing, ultraviolet absorbing and antioxidant effects.
The buddleja officinalis extract contains a large amount of yellow pigments, has a strong absorption peak at a wavelength of 400-500, has a good synergistic blue light resistance effect in the composition, contains various amino acids, trace elements, A-crocin, 4, 5, 7-trihydroxyflavone, mannitol, buddlejasaponins and other components, has nutrition and health care effects on a human body, and can be directly used without purification. The inventor also finds that the crude extract of the yellow pigment of the buddleja officinalis contains active ingredients for removing DPPH free radicals, and researches on the buddleja officinalis total flavonoids show that the buddleja officinalis has stronger effect of removing DPPH free radicals than VC, and the buddleja officinalis total flavonoids have the effect of removing hydroxyl free radicals and have the effect of inhibiting the lipid peroxidation of lecithin induced by the hydroxyl free radicals.
The rhodiola rosea extract contains a large amount of anti-aging active superoxide dismutase (SOD), salidroside tyrosol, rhodiola rosea lactone, 35 microelements such as calcium, phosphorus, iron, zinc, manganese and the like with biological activity, 18 amino acids (8 of which are essential amino acids which can not be synthesized by human bodies), vitamin A, D, E and other more than 100 chemical components, and has natural ultraviolet radiation resistance and ionizing radiation resistance and strong free radical scavenging capacity.
The phenylethanol total glycosides in herba cistanches extract has effects of whitening skin and preventing sunburn, and can inhibit tyrosinase activity and reduce melanogenesis.
Tyrosinase is a rate-limiting enzyme for catalyzing melanogenesis in melanocytes, and the total phenylethanol glycoside extracted from cistanche deserticola can permeate into skin, effectively inhibit the activity of tyrosinase in the skin and block the formation of melanin while not influencing the cell proliferation concentration, thereby reducing skin pigmentation, removing stains and freckles, and not generating toxic, irritant, sensitizing and other side effects on the melanocytes.
The catalytic action of tyrosinase and dopaquinone and subsequent autoxidation reaction all need the participation of free radicals, the number of the free radicals is increased or the activity is enhanced, and the melanogenesis speed can be accelerated. The cistanche deserticola extract, the butterflybush flower extract and the rhodiola rosea extract have the effects of scavenging free radicals and resisting oxidation, and further inhibit the catalytic action of tyrosinase and dopaquinone and subsequent automatic oxidation reaction through synergistic action, so that the generation speed of melanin is inhibited, the damage of ultraviolet, infrared, blue light and the like to the skin is reduced, a good all-optical protection effect is achieved, and the photoaging of the skin is slowed down.
The photo-protection plant extract composition provided by the invention has a synergistic effect, can realize effective absorption and blocking of ultraviolet rays, blue light and infrared rays, has a good all-optical protection effect, and can effectively repair sensitive skin. In order to realize better effects of resisting ultraviolet blue light, resisting infrared rays and slowing down skin photoaging, the photoprotective plant extract composition also comprises the following components: caulis Seu folium Lespedezae Bicoloris extract, herba Viciae Cylindricae extract, flos Tagetis Erectae extract, calyx Seu fructus Physalis extract, and Cistus extract.
The effect of the lespedeza extract is as follows: lespedeza (Lespedeza capitata) is a long-history traditional Chinese herbal medicine also called round-head clover, belongs to leguminous plants, regulates and controls blue-light mediated oxidative stress, limits blue-light injury by enhancing the Nrf2 approach, reduces the accumulation of hydroxylated proteins, and reduces inductivity. And can protect the skin by reducing free radicals produced by induced oxidative stress.
The function of the physalis alkekengi calyx extract is as follows: physalis, commonly known as lantern, is a root-like perennial plant with bitter and sweet red orange fruit and calyx. Phytosterols are considered to be the main class of compounds found in the physalis species, phytosterols being a natural steroid found in a class of plants, with a chemical structure similar to that of cholesterol. The phytosterol blend can be used as antioxidant to reduce lipid peroxidation and scavenge free radicals. In addition, studies have shown the ability of phytosterols to neutralize Reactive Oxygen Species (ROS).
The function of the rosa roxburghii extract is as follows: the skin care product is rich in a natural antioxidant molecule, namely Myricetin glycoside, and the substance can protect the skin from damage caused by sunlight, accelerate the renewal of epidermis and save the skin from exposure to multiple sunlight. Collagen and collagen fibers are protected from blue light radiation from the sun or electronic products. Reducing free radicals and oxidative stress caused by UVA and UVB. And the skin care product can relieve damages caused by ultraviolet light, blue light and infrared light, synthesizes more recovery protein 1, ECM1 (extracellular matrix protein 1) in keratinocytes, helps skin recovery and accelerates epidermal renewal.
The function of the wild soybean extract is as follows: the extract of Glycine max Merr contains gamma-oryzanol as photoprotectant, phytosterol has antiinflammatory effect, phenolic acid (ferulic acid, salicylic acid) is chelating agent, and the extract of Glycine max Merr contains photoprotectan and antioxidant, and has strong antiinflammatory and soothing effects against free radicals generated by ultraviolet, blue light and red light. Photodamage stimulation induces the expression of the COX-2 (cyclooxygenase-2) gene, which primarily controls inflammatory markers, and the wild soybean extract can effectively inhibit the inflammatory factor.
The marigold extract has the following functions: the marigold extract contains lutein, has strong oxidation resistance, can directly absorb blue light of 400-500nm, can absorb free radicals caused by the blue light, and delays skin aging.
The plant extracts of the invention can be purchased in the market or prepared by extraction.
Preferably, the photoprotective plant extract composition comprises the following components in parts by mass: 1-15 parts of a malus extract, 10-30 parts of a buddleja officinalis extract, 10-30 parts of a rhodiola rosea extract and 20-50 parts of a cistanche salsa extract.
More preferably, the composition comprises 7-12 parts of malus extract, 15-25 parts of buddleja officinalis extract, 15-25 parts of rhodiola rosea extract and 25-40 parts of cistanche salsa extract.
More preferably, the composition comprises the following components in parts by mass: 10 parts of apple extract, 20 parts of butterflybush flower extract, 20 parts of rhodiola rosea extract and 30 parts of cistanche salsa extract.
Preferably, the photoprotective plant extract composition comprises the following components in parts by mass: 1-15 parts of a malus extract, 10-30 parts of a buddleja officinalis extract, 10-30 parts of a rhodiola rosea extract, 20-50 parts of a cistanche deserticola extract, 1-50 parts of a lespedeza extract, 1-50 parts of a wild soybean extract, 1-50 parts of a marigold extract, 1-50 parts of a wintercherry calyx extract and 1-50 parts of a rosa multiflora extract.
Preferably, 7-12 parts of malus extract, 15-25 parts of butterflybush flower extract, 15-25 parts of rhodiola rosea extract, 25-40 parts of cistanche deserticola extract, 1-20 parts of lespedeza extract, 1-20 parts of wild soybean extract, 1-20 parts of marigold extract, 1-20 parts of wintercherry calyx extract and 1-20 parts of rosa multiflora extract.
More preferably, the composition comprises the following components in parts by mass: 10 parts of apple extract, 20 parts of butterflybush flower extract, 20 parts of rhodiola extract, 30 parts of herba cistanches extract, 10 parts of lespedeza extract, 10 parts of wild soybean extract, 10 parts of marigold extract, 10 parts of wintercherry extract and 10 parts of rock rose extract.
The application of the photo-protection plant extract composition in preparing the anti-ultraviolet, anti-blue-light and anti-infrared photo-protection preparation is also within the protection scope of the invention.
The application of the light protection plant extract composition in preparing the sunscreen preparation with the effects of reducing the generation of active oxygen and scavenging free radical DPPH is also within the protection scope of the invention.
The application of the light protection plant extract composition in preparing the sunscreen preparation with the effects of inhibiting matrix metalloproteinase MMP degradation and promoting collagen generation is also within the protection scope of the invention.
The invention also provides a sunscreen preparation which is prepared from the photoprotective plant extract composition and other acceptable auxiliary materials.
Wherein, in the sunscreen preparation, the mass content of the photoprotective plant extract composition is 1-50%. For example, it may be 1%, 5% or 20%, more preferably 5%.
Compared with the prior art, the invention has the beneficial effects that:
the photo-protection plant extract composition effectively absorbs ultraviolet light and blue light through the synergistic effect of the components, has excellent anti-ultraviolet and anti-blue light effects, can effectively inhibit the increase of matrix metalloproteinase MMP synthesis caused by red light induction, promotes the generation of collagen, effectively resists the generation of free radicals, can achieve the comprehensive photo-protection effect, is beneficial to repairing the damage of radiation to the skin and relieves the skin sensitivity problem.
Drawings
Fig. 1 is a photograph of whitening effect before and after using a sample by volunteers.
FIG. 2 is a photograph of the soothing effect before and after the sample was used by the volunteer.
Detailed Description
The present invention will be further described with reference to specific embodiments, but the present invention is not limited to the examples in any way. The starting reagents employed in the examples of the present invention are, unless otherwise specified, those that are conventionally purchased.
Example 1
A photoprotective plant extract composition comprising the following components: 1 part of apple extract, 10 parts of butterflybush flower extract, 10 parts of rhodiola extract and 10 parts of cistanche extract.
Example 2
A photoprotective plant extract composition comprising the following components: 15 parts of apple extract, 30 parts of butterflybush flower extract, 30 parts of rhodiola extract and 50 parts of cistanche extract.
Example 3
A photoprotective plant extract composition comprising the following components: 10 parts of apple extract, 20 parts of butterflybush flower extract, 20 parts of rhodiola rosea extract and 30 parts of cistanche salsa extract.
Example 4
A photoprotective plant extract composition comprising the following components: 7 parts of apple extract, 15 parts of butterflybush flower extract, 15 parts of rhodiola rosea extract and 25 parts of cistanche salsa extract.
Example 5
A photoprotective plant extract composition comprising the following components: 12 parts of apple extract, 25 parts of butterflybush flower extract, 25 parts of rhodiola rosea extract and 40 parts of cistanche salsa extract.
Example 6
A photoprotective plant extract composition comprising the following components: 1 part of apple extract, 10 parts of butterflybush flower extract, 10 parts of rhodiola rosea extract, 10 parts of herba cistanches extract, 1 part of lespedeza extract, 1 part of wild soybean extract, 1 part of marigold extract, 1 part of physalis calyx extract and 1 part of cistanches extract.
Example 7
A photoprotective plant extract composition comprising the following components: 15 parts of apple extract, 30 parts of butterflybush flower extract, 30 parts of rhodiola rosea extract, 50 parts of herba cistanches extract, 50 parts of lespedeza extract, 50 parts of wild soybean extract, 50 parts of marigold extract, 50 parts of wintercherry extract and 50 parts of rock rose extract.
Example 8
A photoprotective plant extract composition comprising the following components: 10 parts of apple extract, 20 parts of butterflybush flower extract, 20 parts of rhodiola extract, 30 parts of herba cistanches extract, 10 parts of lespedeza extract, 10 parts of wild soybean extract, 10 parts of marigold extract, 10 parts of wintercherry extract and 10 parts of rock rose extract.
Example 9
A photoprotective plant extract composition comprising the following components: 10 parts of apple extract, 20 parts of butterflybush flower extract, 20 parts of rhodiola extract, 30 parts of herba cistanches extract, 20 parts of lespedeza extract, 20 parts of wild soybean extract, 20 parts of marigold extract, 20 parts of wintercherry extract and 20 parts of rock rose extract.
Example 10
A cream comprises the following specific components, wherein the photoprotective plant extract composition of example 3 is contained, and the content of the photoprotective plant extract composition is 1%.
TABLE 1 specific Components of the cream
Figure BDA0002581848240000071
Example 11
A cream comprises the specific components shown in Table 1, wherein the photoprotective plant extract composition of example 3 is contained in an amount of 5%.
Example 12
A cream comprises the specific components shown in Table 1, wherein the photoprotective plant extract composition of example 3 is contained in an amount of 20%.
Comparative example 1
A photoprotective plant extract composition comprising the following components: 20 parts of butterflybush flower extract, 20 parts of rhodiola rosea extract and 30 parts of cistanche salsa extract.
Comparative example 2
A photoprotective plant extract composition comprising the following components: 10 parts of apple extract, 20 parts of rhodiola extract and 30 parts of cistanche extract.
Comparative example 3
A photoprotective plant extract composition comprising the following components: 10 parts of apple extract, 20 parts of butterflybush flower extract and 30 parts of minced meat floss extract.
Comparative example 4
A photoprotective plant extract composition comprising the following components: 10 parts of apple extract, 20 parts of butterflybush flower extract and 20 parts of rhodiola extract.
Result detection
(1) Active oxygen production amount detection
Human fibroblasts were pretreated for 24 hours, then the cells were irradiated with artificial UVA \ UVB, pictures were taken using a fluorescence microscope, the intracellular reactive oxygen species ROS were quantified, and the percentage of decrease in the amount of reactive oxygen species generated under artificial UVA \ UVB by the photo-protective plant compositions of examples and comparative examples was tested for human fibroblasts, respectively.
The results are shown in table 2 below:
TABLE 2
Figure BDA0002581848240000081
Figure BDA0002581848240000091
Comparative examples 1-4 the percent reduction in active oxygen production of the photoprotective compositions was much lower than that of the photoprotective composition of example 3, indicating that the components work synergistically, and that apple extract, butterflybush flower extract, and minced meat floss extract must be combined, but none of them work synergistically, to significantly reduce the active oxygen produced by UVA \ UVB radiation, and to significantly reduce the skin damage caused by UVA \ UVB light.
(2) Blue light resistance detection test
The blue light resistance detection tests of the application examples 1-9, the application comparative examples 1-4 and the blank control group are carried out in the test example, the test blank group, the test example and the comparative example test instrument and the test method are the same, and the difference is only that the blank control group (without LED (476nm, 1900lux) blue light irradiation) is added, and the test results of the blue light resistance detection tests are shown in Table 3.
TABLE 3
Figure BDA0002581848240000092
Figure BDA0002581848240000101
The results in table 3 show that the activity of human epidermal keratinocytes and the activity of human dermal fibroblasts in examples 2-3, 5-6 containing the photo-protective composition are more than 98%, which indicates that the photo-protective composition has a very strong blue light resistance and can effectively resist the damage of blue light to the skin. And the activity was also more than 90% for the 8.9 ratio cells. Comparative examples 1 to 4, in which the activity of human epidermal keratinocytes and the activity of human dermal fibroblasts are relatively low, show that the active ingredients in the photo-protective composition of the present invention not only exert their own effects, but also supplement each other and synergize synergistically, so that the skin damaged by blue light is protected while the skin is protected from the damage of blue light, the effect of repairing the skin damaged by blue light is achieved, and the resistance of the skin to blue light is improved.
(2) Detection of Effect of promoting collagen I production
The method for detecting the collagen production rate comprises the following steps:
the test blanks, examples and comparative examples the test instruments and test methods were identical except that a blank control (no LED (625nm, 2000lux) red illumination) was added. And (4) carrying out sample determination according to the instruction of the human type I collagen enzyme-linked immunoassay kit. After human skin fibroblasts were pretreated with the photo-protective composition of the above different examples for 24 hours, the supernatant of the cell culture was collected and centrifuged at 1000 rpm for 15min at 2-8 ℃ and the supernatant was immediately subjected to measurement. Adding a sample and a collagen standard into an enzyme-labeled hole coated with an antibody in advance, adding a recognition antigen marked by horseradish peroxidase (HRP), incubating for 1h at 37 ℃, competitively combining the two with an in-phase antigen to form an immune complex, washing by PBST, enabling the combined HRP to catalyze Tetramethylbenzidine (TMB) to turn blue, then turning yellow under the action of acid, and measuring the absorbance at the wavelength of 450 nm. The collagen production rate was calculated.
The specific detection results are shown in table 5:
TABLE 5
Type I collagen production Rate (%)
Substrate group 35%
Blank group 100%
Example 1 82%
Example 2 98%
Example 3 98%
Example 4 96%
Example 5 97%
Example 6 83%
Example 7 99%
Example 8 98%
Example 9 98%
Example 10 53%
Example 11 95%
Example 12 97%
Comparative example 1 78%
Comparative example 2 76%
Comparative example 3 79%
Comparative example 4 77%
Under the influence of artificial red light, the synthesis of type I collagen is significantly reduced. However, the synthesis of type I collagen was hardly affected by the cells exposed to red light after addition of the photoprotective composition. The synthesis rate of type I collagen of examples 2-3, 5-6 containing the photo-protective composition was above 98%, indicating that the photo-protective composition has a very strong anti-red effect and can still synthesize type I collagen under the irradiation of red light. Can effectively resist the damage of blue light to the skin.
(3) Radical scavenging DPPH assay
The detection method comprises the following steps: the ultraviolet and blue light red light irradiation can lead the organism to generate a large amount of free radicals, the free radicals are active chemicals with extremely strong oxidizing capability, and the large amount of free radicals can cause damage to the skin, such as accelerating the formation of color spots and melanin. DPPH is a stable free radical whose light absorption is reduced by pairing with its single electron in the presence of a free radical scavenger, thereby evaluating the ability of a substance to scavenge free radicals and thus assess its antioxidant capacity. The absorbance is measured by DPPH free radical scavenging test.
The results of the measurement are shown in table 3 below:
TABLE 3
DPPH radical scavenging ratio (%)
Substrate group 0%
Example 1 83%
Example 2 99%
Example 3 98%
Example 4 95%
Example 5 98%
Example 6 85%
Example 7 99%
Example 8 98%
Example 9 98%
Example 10 43%
Example 11 95%
Example 12 96%
Comparative example 1 73%
Comparative example 2 70%
Comparative example 3 75%
Comparative example 4 78%
The human body using effect of the specific sample of the invention is the using effect of volunteers shown in figure 1 and figure 2, and has the effect of relieving and whitening.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims (10)

1. A photoprotective plant extract composition comprising the following components: apple extract, butterflybush flower extract, rhodiola extract, and cistanche extract.
2. The photoprotective plant extract composition of claim 1, further comprising the following components: caulis Seu folium Lespedezae Bicoloris extract, herba Viciae Cylindricae extract, flos Tagetis Erectae extract, calyx Seu fructus Physalis extract, and Cistus extract.
3. The photoprotective plant extract composition of claim 1, comprising the following components in parts by mass: 1-15 parts of a malus extract, 10-30 parts of a buddleja officinalis extract, 10-30 parts of a rhodiola rosea extract and 20-50 parts of a cistanche salsa extract.
4. The photoprotective plant extract composition of claim 3, comprising the following components in parts by mass: 10 parts of apple extract, 20 parts of butterflybush flower extract, 20 parts of rhodiola rosea extract and 30 parts of cistanche salsa extract.
5. The photoprotective plant extract composition of claim 2, comprising the following components in parts by mass: 1-15 parts of a malus extract, 10-30 parts of a buddleja officinalis extract, 10-30 parts of a rhodiola rosea extract, 20-50 parts of a cistanche deserticola extract, 1-50 parts of a lespedeza extract, 1-50 parts of a wild soybean extract, 1-50 parts of a marigold extract, 1-50 parts of a wintercherry calyx extract and 1-50 parts of a rosa multiflora extract.
6. Use of a photoprotective plant extract composition of any one of claims 1 to 5 in the preparation of a UV-, blue-and IR-resistant photoprotective preparation.
7. Use of a photoprotective plant extract composition of any one of claims 1 to 5 in the preparation of a sunscreen formulation effective in reducing reactive oxygen species generation and scavenging free radical DPPH.
8. Use of the photoprotective plant extract composition of any one of claims 1 to 5 in the preparation of a sunscreen formulation effective in inhibiting degradation of matrix metalloproteinases MMP and promoting collagen production.
9. A sunscreen preparation, characterized by being prepared from the photoprotective plant extract composition of any one of claims 1 to 5 and other acceptable adjuvants.
10. The sunscreen formulation of claim 9 wherein the photoprotective plant extract composition comprises from 1% to 50% by weight of the sunscreen formulation.
CN202010669767.9A 2020-07-13 2020-07-13 Photo-protection plant extraction composition and application thereof Pending CN111821235A (en)

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