CN111778136A - Multifunctional cell culture solution providing device - Google Patents
Multifunctional cell culture solution providing device Download PDFInfo
- Publication number
- CN111778136A CN111778136A CN202010597555.4A CN202010597555A CN111778136A CN 111778136 A CN111778136 A CN 111778136A CN 202010597555 A CN202010597555 A CN 202010597555A CN 111778136 A CN111778136 A CN 111778136A
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- Prior art keywords
- liquid outlet
- cell culture
- culture solution
- culture container
- outlet pipe
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- 238000004113 cell culture Methods 0.000 title claims abstract description 33
- 239000007788 liquid Substances 0.000 claims abstract description 54
- 238000000429 assembly Methods 0.000 claims abstract description 10
- 230000000712 assembly Effects 0.000 claims abstract description 10
- 239000002245 particle Substances 0.000 claims abstract description 7
- 125000003003 spiro group Chemical group 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000000889 atomisation Methods 0.000 abstract description 9
- 210000004027 cell Anatomy 0.000 description 13
- 238000002474 experimental method Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 7
- 230000035764 nutrition Effects 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000012010 growth Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 238000007906 compression Methods 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a multifunctional cell culture solution supply device which comprises a culture container, wherein a transverse support shaft is rotatably supported on the outer side of the culture container, a driving groove is formed in the outer end of the transverse support shaft, a plurality of ball pushing assemblies are uniformly arranged on the outer side of the culture container around the transverse support shaft, a circle of positioning grooves are uniformly formed in the outer side of the transverse support shaft, a rotary table is fixedly supported by the transverse support shaft, a plurality of liquid outlets are embedded in a disc body of the rotary table, a sleeve disc is sleeved on the outer side of the rotary table, an inner liquid outlet pipe is communicated between the inner side of the sleeve disc and the culture container, the inner side of each liquid outlet pipe is abutted against an end plate on the inner side of the sleeve disc, a liquid inlet convenient to be communicated with the inner liquid outlet pipe is formed in the inner side of each liquid outlet pipe, the liquid. The invention adds the atomization function on the basis of providing the liquid discharge of the cell culture solution, and can select the atomization nozzles with different atomization particle diameters.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a multifunctional cell culture solution supply device.
Background
With the development of biotechnology, the field of cell culture is rapidly developed, cell culture is an important branch of regenerative medicine and life science, when culturing cells, a cell culture device is concerned with important factors for culturing cells, and in the culture of cells, a cell culture solution is a basic substance for supplying cell nutrition and promoting cell reproduction and proliferation in the cultured cells and is also a living environment for the growth and proliferation of the cultured cells, so that the cell culture solution needs to be detected and extracted when in use. The conventional device for supplying cell culture solution in real time also has the problems that the device is inconvenient to adjust and place bottles with different sizes for use and does not have the function of collecting residual liquid.
Therefore, the patent specification with the publication number of CN210560488U discloses a novel cell culture solution real-time providing device, which comprises a base, a bottom plate, a culture bottle, a bottle cavity, a bottle cap, an upper communicating head, a middle communicating head, a lower communicating head, a first manual valve, a first liquid outlet pipe, a second manual valve, a second liquid outlet pipe, a third manual valve, a third liquid outlet pipe, a providing bottle, an adjusting support plate frame structure and a collecting, dismounting and mounting box structure, wherein a bottom plate bolt is installed on the upper surface of the base; the culture bottles are integrally arranged at the upper end of the bottom plate; the bottle cap is in threaded connection with the upper part of the culture bottle; the upper, middle and lower connecting heads are respectively and integrally arranged at the upper part, the middle part and the lower part of the right side of the culture bottle.
However, the cell culture solution supply device has the defects in use, does not have an atomization function, can not select atomization nozzles with different atomization particle sizes according to needs, and can not ensure the nutrition required by cell survival and normal growth in an experiment, can not immerse the whole cell in the cell culture solution too much to influence the experiment effect, and can ensure the normal activity of the cell in an infection experiment. Therefore, it is necessary to optimize and improve it.
Disclosure of Invention
The present invention has been made to overcome the above problems occurring in the conventional art, and an object of the present invention is to provide a multi-functional cell culture solution supply apparatus.
In order to achieve the technical purpose and achieve the technical effect, the invention is realized by the following technical scheme:
a multifunctional cell culture solution providing device comprises a culture container, wherein a transverse supporting shaft is rotatably supported outside the culture container, a driving groove is arranged at the outer end of the transverse supporting shaft, a plurality of heading ball assemblies are uniformly arranged outside the culture container around the transverse supporting shaft, a circle of positioning groove matched with the heading ball assemblies is uniformly arranged outside the transverse supporting shaft, a rotary disc is fixedly supported on a shaft body of the transverse supporting shaft outside the heading ball assemblies, a plurality of liquid outlets are embedded in a disc body of the rotary disc, a sleeve disc is sleeved outside the rotary disc, an inner liquid outlet pipe is communicated between the inner side of the sleeve disc and the culture container, an opening valve is mounted on a pipe body of the inner liquid outlet pipe, the inner side of each liquid outlet head is abutted against an inner side end plate of the sleeve disc, a liquid inlet conveniently communicated with the inner liquid outlet pipe is arranged on the inner side of each liquid outlet head, and the liquid inlet of each liquid outlet pipe can be communicated with the, and a tray component is arranged below the sleeve tray of the culture container.
Furthermore, in the above multifunctional cell culture solution providing device, an included angle between two adjacent ball pushing assemblies is equal to an included angle between two adjacent liquid outlet heads.
Furthermore, in the multifunctional cell culture solution providing device, four ball pushing assemblies and four liquid outlet heads are respectively arranged.
Furthermore, in the above multifunctional cell culture solution supply device, the liquid outlet head comprises a common liquid outlet head, and the rest are atomizing nozzles, and the atomizing particle size of the atomizing nozzles gradually decreases.
Further, among the multi-functional device that provides of above-mentioned cell culture liquid, the tray subassembly is including being fixed in the supporting seat in the culture container outside, the direction spout has been seted up to the supporting seat outside, the slip restriction has the tray in the direction spout, the downside of tray is equipped with the convex seat, the below that the supporting seat outside is located the direction spout is fixed with the layer board, the spiro union has the screw rod of increaseing in the layer board, the top rotation restriction of screw rod of increaseing is in the convex seat.
Further, in the multifunctional cell culture solution supply device, a water receiving flange is arranged at the edge of the upper end of the tray.
The invention has the beneficial effects that:
the invention has reasonable structural design, the provided device can realize the height adjustment of the tray, the atomization function is added on the basis of providing the liquid discharge of the cell culture solution, the atomization nozzles with different atomization particle diameters can be selected, the nutrition required by the survival and normal growth of cells in an experiment can be ensured, the experiment effect cannot be influenced by too wet whole cells immersed in the cell culture solution, and the normal activity of the cells in an infection experiment can be ensured.
Of course, it is not necessary for any one product that embodies the invention to achieve all of the above advantages simultaneously.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural view of the present invention as a whole;
FIG. 2 is a schematic view of the construction of the lateral support shaft and ball ejection assembly of the present invention;
FIG. 3 is a left side view of the structure of the sleeve disk of the present invention;
FIG. 4 is a right side view of the structure of the sleeve disk of the present invention;
in the drawings, the parts are numbered as follows:
1-culture vessel, 2-transverse support shaft, 3-drive groove, 4-heading ball component, 401-sleeve, 402-compression spring, 403-heading ball, 5-rotary disc, 6-liquid outlet head, 7-sleeve disc, 8-inner liquid outlet pipe, 9-opening valve, 10-support seat, 11-guide sliding groove, 12-tray, 13-convex seat, 14-supporting plate and 15-height-adjusting screw rod.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, the present embodiment is a multifunctional cell culture solution supply device, which includes a culture container 1, a transverse support shaft 2 rotatably supported on the outer side of the culture container 1, a driving groove 3 disposed at the outer end of the transverse support shaft 2, and a plurality of top ball assemblies 4 uniformly disposed around the transverse support shaft 2 on the outer side of the culture container 1. The outside of horizontal back shaft 2 evenly is equipped with round and pushes up ball assembly 4 complex constant head tank, and horizontal back shaft 2 is located the outer axis body fixed support of pushing up ball assembly 4 and has carousel 5. A plurality of liquid outlet heads 6 are embedded in the plate body of the rotary plate 5, a sleeve plate 7 is sleeved outside the rotary plate 5, an inner liquid outlet pipe 8 is communicated between the inner side of the sleeve plate 7 and the culture container 1, and an opening valve 9 is arranged on the pipe body of the inner liquid outlet pipe 8. The inboard of liquid outlet head 6 offsets with the inboard end plate of set 7, and the inboard of liquid outlet head 6 is equipped with the inlet of being convenient for with interior drain pipe 8 switch-on, can make the inlet of liquid outlet head 6 and interior drain pipe 8 switch-on through rotary table 5, cultivates container 1 and is equipped with tray assembly in the below of set 7.
In this embodiment, an included angle between two adjacent ball pushing assemblies 4 is equal to an included angle between two adjacent liquid outlet heads 6. The ball-ejecting assembly 4 comprises a sleeve 401, the inner cavity of the sleeve 401 supports a ball-ejecting member 403 through a compression spring 402, and the open end of the sleeve 401 is provided with a baffle plate which prevents the ball-ejecting member 403 from being completely exposed.
In this embodiment, four ball pushing assemblies 4 and four liquid outlet heads 6 are respectively arranged. The liquid outlet head 6 comprises a common liquid outlet head, and the rest are atomizing nozzles, and the atomizing particle size of the atomizing nozzles gradually becomes smaller.
In this embodiment, the tray assembly includes the supporting seat 10 that is fixed in the culture container 1 outside, and guide chute 11 has been seted up in the supporting seat 10 outside, and the restriction of sliding has tray 12 in the guide chute 11, and the downside of tray 12 is equipped with convex seat 13, and the supporting seat 10 outside is located the below of guide chute 11 and is fixed with layer board 14, and the spiro union has heightening screw rod 15 in layer board 14, and heightening screw rod 15's top is rotated the restriction in convex seat 13, and the upper end edge of tray 12 is equipped with the water receiving flange.
One specific application of this embodiment is: this embodiment structural design is reasonable, and it provides the device and can realize the regulation of tray height, increases the atomizing function on the basis that provides cell culture liquid ejection of compact, can need select the atomizer of different atomizing particle diameters, realizes both guaranteeing cell survival and the required nutrition of normal growth in the experiment, can not immerse cell whole again in cell culture liquid too moist influence experiment effect, and it is normal to keep guaranteeing cell activity in the contamination experiment.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (6)
1. A multifunctional cell culture solution supply device comprises a culture container, and is characterized in that: a transverse supporting shaft is rotatably supported at the outer side of the culture container, a driving groove is arranged at the outer end of the transverse supporting shaft, a plurality of heading ball components are uniformly arranged on the outer side of the culture container around the transverse supporting shaft, a circle of positioning grooves matched with the heading ball components are uniformly arranged on the outer side of the transverse supporting shaft, a rotary table is fixedly supported on a shaft body of the transverse support shaft positioned outside the ball ejecting assembly, a plurality of liquid outlet heads are embedded in a disc body of the rotary table, the outer side of the rotary disc is sleeved with a sleeve disc, the inner side of the sleeve disc is communicated with an inner liquid outlet pipe between the culture container and the inner side of the sleeve disc, the body of the inner liquid outlet pipe is provided with an opening valve, the inner side of the liquid outlet head is abutted against the end plate at the inner side of the sleeve disc, the inner side of the liquid outlet head is provided with a liquid inlet which is conveniently communicated with the inner liquid outlet pipe, through rotating the carousel can make the inlet of liquid outlet head and interior drain pipe switch-on, the below that is located the set dish of culture vessel is equipped with tray assembly.
2. The multi-purpose supplying apparatus of cell culture solution according to claim 1, characterized in that: and the included angle between every two adjacent ball ejecting components is equal to the included angle between every two adjacent liquid outlet heads.
3. The multi-purpose supplying apparatus of cell culture solution according to claim 2, characterized in that: four ball pushing assemblies and four liquid outlet heads are arranged respectively.
4. The multi-purpose supplying apparatus of cell culture solution according to claim 1, characterized in that: the liquid outlet head comprises a common liquid outlet head, the rest of the liquid outlet head is an atomizing nozzle, and the atomizing particle size of the atomizing nozzle is gradually reduced.
5. The multi-purpose supplying apparatus of cell culture solution according to claim 1, characterized in that: the tray subassembly is including being fixed in the supporting seat in the culture container outside, the direction spout has been seted up in the supporting seat outside, the restriction of sliding has the tray in the direction spout, the downside of tray is equipped with the convex seat, the supporting seat outside is located the below of direction spout and is fixed with the layer board, the spiro union has the screw rod of increaseing in the layer board, the top rotation restriction of screw rod of increaseing is in the convex seat.
6. The multi-purpose supplying apparatus of cell culture solution according to claim 5, wherein: and a water receiving flange is arranged at the edge of the upper end of the tray.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010597555.4A CN111778136A (en) | 2020-06-28 | 2020-06-28 | Multifunctional cell culture solution providing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010597555.4A CN111778136A (en) | 2020-06-28 | 2020-06-28 | Multifunctional cell culture solution providing device |
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| Publication Number | Publication Date |
|---|---|
| CN111778136A true CN111778136A (en) | 2020-10-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010597555.4A Pending CN111778136A (en) | 2020-06-28 | 2020-06-28 | Multifunctional cell culture solution providing device |
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| Country | Link |
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| CN (1) | CN111778136A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102010822A (en) * | 2010-09-14 | 2011-04-13 | 天津开发区合普工贸有限公司 | Cell culture fluid atomizing supply device |
| US20120125483A1 (en) * | 2009-08-07 | 2012-05-24 | Aes Chemunex | Device and method for dispensing a product into a petri dish |
| CN204816945U (en) * | 2015-06-07 | 2015-12-02 | 南通均元化工科技有限公司 | Multi -functional plastics atomizer |
| CN208449640U (en) * | 2018-06-08 | 2019-02-01 | 广州市德天环保科技有限公司 | A kind of automatic atomising nozzle arrangements |
| CN210560488U (en) * | 2019-08-20 | 2020-05-19 | 天津斯坦姆生物科技有限公司 | Novel cell culture solution provides in real time device |
-
2020
- 2020-06-28 CN CN202010597555.4A patent/CN111778136A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120125483A1 (en) * | 2009-08-07 | 2012-05-24 | Aes Chemunex | Device and method for dispensing a product into a petri dish |
| CN102010822A (en) * | 2010-09-14 | 2011-04-13 | 天津开发区合普工贸有限公司 | Cell culture fluid atomizing supply device |
| CN204816945U (en) * | 2015-06-07 | 2015-12-02 | 南通均元化工科技有限公司 | Multi -functional plastics atomizer |
| CN208449640U (en) * | 2018-06-08 | 2019-02-01 | 广州市德天环保科技有限公司 | A kind of automatic atomising nozzle arrangements |
| CN210560488U (en) * | 2019-08-20 | 2020-05-19 | 天津斯坦姆生物科技有限公司 | Novel cell culture solution provides in real time device |
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Application publication date: 20201016 |