CN111487240A - Special EPI diagnostic reagent for animals - Google Patents
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- CN111487240A CN111487240A CN202010269182.8A CN202010269182A CN111487240A CN 111487240 A CN111487240 A CN 111487240A CN 202010269182 A CN202010269182 A CN 202010269182A CN 111487240 A CN111487240 A CN 111487240A
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 155
- 241001465754 Metazoa Species 0.000 title claims abstract description 52
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims abstract description 36
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 34
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 34
- 239000012498 ultrapure water Substances 0.000 claims abstract description 34
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000011780 sodium chloride Substances 0.000 claims abstract description 17
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 13
- 239000008103 glucose Substances 0.000 claims abstract description 13
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 claims abstract description 9
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 5
- FHNINJWBTRXEBC-UHFFFAOYSA-N Sudan III Chemical group OC1=CC=C2C=CC=CC2=C1N=NC(C=C1)=CC=C1N=NC1=CC=CC=C1 FHNINJWBTRXEBC-UHFFFAOYSA-N 0.000 claims description 10
- 229940099373 sudan iii Drugs 0.000 claims description 10
- 239000003755 preservative agent Substances 0.000 claims description 8
- 230000002335 preservative effect Effects 0.000 claims description 8
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- 229910052740 iodine Inorganic materials 0.000 claims description 3
- 239000011630 iodine Substances 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical group [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 claims description 3
- 229940033663 thimerosal Drugs 0.000 claims description 3
- 238000003745 diagnosis Methods 0.000 abstract description 10
- 201000010099 disease Diseases 0.000 abstract description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 9
- 230000000007 visual effect Effects 0.000 abstract description 3
- 229920002472 Starch Polymers 0.000 description 10
- 239000008107 starch Substances 0.000 description 10
- 235000019698 starch Nutrition 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 8
- 238000005303 weighing Methods 0.000 description 8
- 210000003608 fece Anatomy 0.000 description 5
- 239000008187 granular material Substances 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 230000002421 anti-septic effect Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 239000000644 isotonic solution Substances 0.000 description 4
- 239000006059 cover glass Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 208000035467 Pancreatic insufficiency Diseases 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 201000007089 exocrine pancreatic insufficiency Diseases 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 239000012046 mixed solvent Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- RCTGMCJBQGBLKT-PAMTUDGESA-N scarlet red Chemical compound CC1=CC=CC=C1\N=N\C(C=C1C)=CC=C1\N=N\C1=C(O)C=CC2=CC=CC=C12 RCTGMCJBQGBLKT-PAMTUDGESA-N 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000001810 trypsinlike Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
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- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Engineering & Computer Science (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses an animal special EPI diagnostic reagent, which consists of a first diagnostic reagent A, a second diagnostic reagent B and a third diagnostic reagent C, wherein the first diagnostic reagent A can be matched with the second diagnostic reagent B or the third diagnostic reagent C for use; the first diagnostic reagent A comprises the following components in parts by weight: 0.85-5.5 parts of sodium chloride or glucose and 100 parts of ultrapure water; the second diagnostic reagent comprises the following components in parts by weight: 1-5 parts of iodine, 2-10 parts of potassium iodide and 85-97 parts of ultrapure water; the third diagnostic reagent comprises the following components in parts by weight: 1-2 parts of Sudan dye, 50 parts of 70% ethanol and 50 parts of acetone. The animal special EPI diagnostic reagent fills the blank in the field of animal EPI disease diagnostic reagents at home at present, and is convenient to use, rapid in diagnosis and visual in result.
Description
Technical Field
The invention relates to the technical field of medical biological detection,
in particular, the invention relates to an EPI diagnostic reagent special for animals.
Background
Generally speaking, methods for diagnosing EPI diseases mainly comprise general diagnosis (comprising clinical symptoms, stool examination, blood biochemical examination, blood routine examination) and specific diagnosis (comprising serum trypsin-like immune response (T L I), B12 content and folic acid content), wherein T L I is the confirmed diagnosis index for diagnosing EPI, but the method can only be carried out in foreign laboratories at present, and takes about half a month for a long period.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide an animal special EPI diagnostic reagent.
In order to solve the problems, the invention adopts the following technical scheme:
an animal-specific EPI diagnostic reagent, which consists of a first diagnostic reagent, a second diagnostic reagent and a third diagnostic reagent, wherein the first diagnostic reagent can be matched with the second diagnostic reagent or the third diagnostic reagent for use;
the first diagnostic reagent comprises the following components in parts by weight: 0.85-5.5 parts of sodium chloride or glucose and 100 parts of ultrapure water;
the second diagnostic reagent comprises the following components in parts by weight: 1-5 parts of iodine, 2-10 parts of potassium iodide and 85-97 parts of ultrapure water;
the third diagnostic reagent comprises the following components in parts by weight: 1-2 parts of Sudan dye, 50 parts of 70% ethanol and 50 parts of acetone.
Preferably, the first diagnostic reagent contains the following components in parts by weight: 0.85-0.95 parts of sodium chloride and 100 parts of ultrapure water.
Preferably, the first diagnostic reagent contains the following components in parts by weight: 4.5-5.5 parts of glucose and 100 parts of ultrapure water.
Preferably, the sudan dye is sudan III dye or sudan iv dye.
Preferably, the first diagnostic reagent contains the following components in parts by weight: 0.9 part of sodium chloride and 100 parts of ultrapure water.
Preferably, the first diagnostic reagent contains the following components in parts by weight: 5 parts of glucose and 100 parts of ultrapure water.
Preferably, the third diagnostic reagent contains 1.5 parts of Sudan III dye.
Preferably, the second diagnostic reagent contains the following components in parts by weight: iodine 1.67 parts, potassium iodide 3.33 parts and ultrapure water 95 parts.
Preferably, the first diagnostic reagent further comprises the following components in parts by weight: 0.005-0.015 part of preservative.
Preferably, the preservative is thimerosal sodium.
Compared with the prior art, the invention has the technical effects that:
the special animal EPI diagnostic reagent can be used as a diagnostic reagent for animal pancreatic exocrine Insufficiency (EPI), starch in animal excrement can be qualitatively detected by matching a first diagnostic reagent with a second diagnostic reagent, fat in animal excrement can be qualitatively detected by matching the first diagnostic reagent with a third diagnostic reagent, and the possibility of the animal having the EPI disease is judged by detecting the starch and the fat in the animal excrement. The animal special EPI diagnostic reagent fills the blank in the field of animal EPI disease diagnostic reagents at home at present, and is convenient to use, rapid in diagnosis and visual in result.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
EPI, the Exocrine Pancreatic Insufficiency, causes dyspepsia in animals suffering from EPI diseases, and causes large amounts of starch granules and fat droplets in feces. Based on this, the present invention provides an EPI diagnostic reagent, wherein undigested starch granules and fat granules in feces can be specifically bound with a dye in the EPI diagnostic reagent, thereby generating corresponding colors. The possible diseases can be preliminarily diagnosed by measuring and counting the diameters of the fat particles under a high power microscope.
The embodiment of the invention provides an animal special EPI diagnostic reagent, which consists of a first diagnostic reagent A, a second diagnostic reagent B and a third diagnostic reagent C, wherein the first diagnostic reagent A can be matched with the second diagnostic reagent B or the third diagnostic reagent C for use;
the first diagnostic reagent A comprises the following components in parts by weight: 0.85-5.5 parts of sodium chloride or glucose and 100 parts of ultrapure water;
the second diagnostic reagent comprises the following components in parts by weight: 1-5 parts of iodine, 2-10 parts of potassium iodide and 85-97 parts of ultrapure water;
the third diagnostic reagent comprises the following components in parts by weight: 1-2 parts of Sudan dye, 50 parts of 70% ethanol and 50 parts of acetone.
Wherein, the first diagnostic reagent A is an isotonic solution of sodium chloride or glucose, and when the first diagnostic reagent A is the isotonic solution of sodium chloride, the first diagnostic reagent A contains the following components in parts by weight: 0.85-0.95 parts of sodium chloride and 100 parts of ultrapure water, preferably 0.9 part of sodium chloride; when the first diagnostic reagent A is an isotonic solution of glucose, it contains the following components in parts by weight: glucose 4.5-5.5 parts, and ultrapure water 100 parts, preferably containing glucose 5 parts. Preferably, the first diagnostic reagent a of the present embodiment is an isotonic solution of sodium chloride, which is lower in cost, easy to prepare, and more storage-stable.
When the first diagnostic reagent A is matched with the second diagnostic reagent B or the third diagnostic reagent C for use, the second diagnostic reagent B or the third diagnostic reagent C can be diluted, and meanwhile, the first diagnostic reagent can adjust a detection microenvironment, so that the second diagnostic reagent B and the third diagnostic reagent C are easier to generate a color reaction with starch and fat in excrement.
Preferably, the second diagnostic reagent contains the following components in parts by weight: iodine 1.67 parts, potassium iodide 3.33 parts and ultrapure water 95 parts. The second diagnostic reagent can react with starch in animal excrement, and starch granules are in bluish purple concentric circle radial lines under a high power lens.
The Sudan dye in the third diagnostic reagent can react with fat in animal excrement, and if the Sudan III dye is selected, fat drops are in an orange red round shape under a high power lens, have strong refractivity and are easy to gather at the edge position of a cover glass. If Sudan IV dye is selected, the fat drop is scarlet under high power lens. Preferably, the third diagnostic reagent contains 1.5 parts of sudan III dye.
Preferably, the first diagnostic reagent further comprises the following components in parts by weight: 0.005-0.015 part of preservative. Preferably, the preservative is thimerosal sodium. The preservative can prevent the first diagnostic reagent from deteriorating during repeated use and affecting the detection result.
The detection of animal feces using the EPI diagnostic reagent of this example can be carried out in the following manner:
(1) starch granules in animal feces were detected:
1. 1-2 drops of a first diagnostic reagent A liquid are dripped on the clean glass, abnormal parts of excrement are selected or excrement at different parts is picked up to be directly smeared and inspected.
2. Dripping 2 drops of second diagnostic reagent B, mixing to thin layer, and covering with cover glass.
3. And (6) microscopic observation.
(2) Fat droplets in animal faeces were detected:
1. 1-2 drops of a first diagnostic reagent A liquid are dripped on the clean glass, abnormal parts of excrement are selected or excrement at different parts is picked up to be directly smeared and inspected.
2. Dripping 2 drops of a third diagnostic reagent C, uniformly mixing to a thin layer, and covering with a cover glass.
3. And (6) microscopic observation.
The animal special EPI diagnostic reagent can shorten the EPI disease diagnosis time of animals to about one week, compared with the T L I diagnosis, the diagnosis time can be obviously shortened, and the diagnostic reagent is convenient to use and rapid in diagnosis.
The animal special EPI diagnostic reagent can be used as a diagnostic reagent for animal pancreatic exocrine insufficiency, can qualitatively detect starch in animal excrement by matching the first diagnostic reagent with the second diagnostic reagent, can qualitatively detect fat in animal excrement by matching the first diagnostic reagent with the third diagnostic reagent, and can judge whether the animal has the possibility of EPI diseases or not by detecting the starch and the fat in the animal excrement. The animal special EPI diagnostic reagent fills the blank in the field of animal EPI disease diagnostic reagents at home at present, and is convenient to use, rapid in diagnosis and visual in result.
The composition and preparation method of the special EPI diagnostic reagent for animals are further described in the following with reference to specific examples.
Example 1
The embodiment 1 of the invention provides an animal special EPI diagnostic reagent and a preparation method thereof.
An animal special EPI diagnostic reagent consists of a first diagnostic reagent A, a second diagnostic reagent B and a third diagnostic reagent C.
The first diagnostic reagent A was prepared from 0.9g of sodium chloride, 0.01g of an antiseptic and 100m L g of ultrapure water.
The preparation method comprises weighing ultrapure water with a certain volume, weighing sodium chloride 0.9g, adding into ultrapure water, stirring for 2-4min to dissolve completely, metering to 100m L with ultrapure water, weighing antiseptic 0.01g, adding into the above solution, stirring for 3 min to dissolve completely.
The second diagnostic reagent B was prepared from 1.67g of iodine, 3.33g of potassium iodide and 95m L g of ultrapure water.
The preparation method comprises weighing 95m L ultrapure water, weighing 3.33g potassium iodide, adding into ultrapure water, stirring to dissolve completely, weighing 1.67g iodine, adding into the above solution, and stirring to dissolve completely.
The third diagnostic reagent C is prepared from Sudan III dye 1.5g, 70% ethanol 50m L, and acetone 50m L.
The preparation method comprises weighing 70% ethanol 50m L and acetone 50m L, mixing, weighing Sudan III 1.5g, adding into the above mixed solvent, stirring to dissolve completely, and vacuum filtering with double-layer filter paper.
Example 2
The embodiment 2 of the invention provides an animal special EPI diagnostic reagent and a preparation method thereof.
An animal special EPI diagnostic reagent consists of a first diagnostic reagent A, a second diagnostic reagent B and a third diagnostic reagent C.
The first diagnostic reagent A was prepared from 5g of glucose, 0.01g of an antiseptic and 100m L g of ultrapure water.
The second diagnostic reagent B is composed of 3g of iodine, 6g of potassium iodide and 91m L g of ultrapure water.
The third diagnostic reagent C is prepared from Sudan III dye 2g, 70% ethanol 50m L, and acetone 50m L.
The first diagnostic reagent a, the second diagnostic reagent B, and the third diagnostic reagent C were prepared in the same manner as in example 1.
Example 3
The embodiment 3 of the invention provides an animal special EPI diagnostic reagent and a preparation method thereof.
An animal special EPI diagnostic reagent consists of a first diagnostic reagent A, a second diagnostic reagent B and a third diagnostic reagent C.
The first diagnostic reagent A was prepared from 0.85g of sodium chloride, 0.005g of an antiseptic and 100m L g of ultrapure water.
The second diagnostic reagent B was prepared from 1g of iodine, 3g of potassium iodide and 97m of ultrapure water L.
The third diagnostic reagent C is prepared from Sudan IV dye 1g, 70% ethanol 50m L, and acetone 50m L.
The first diagnostic reagent a, the second diagnostic reagent B, and the third diagnostic reagent C were prepared in the same manner as in example 1.
Example 4
The embodiment 4 of the invention provides an animal special EPI diagnostic reagent and a preparation method thereof.
An animal special EPI diagnostic reagent consists of a first diagnostic reagent A, a second diagnostic reagent B and a third diagnostic reagent C.
The first diagnostic reagent A was prepared from 0.95g of sodium chloride, 0.015g of preservative and 100m L g of ultrapure water.
The second diagnostic reagent B is prepared from 5g of iodine, 10g of potassium iodide and 85m of ultrapure water L.
The third diagnostic reagent C is prepared from Sudan III dye 1g, 70% ethanol 50m L, and acetone 50m L.
The first diagnostic reagent a, the second diagnostic reagent B, and the third diagnostic reagent C were prepared in the same manner as in example 1.
The present invention is not limited to the above-described specific embodiments, and various modifications and variations are possible. Any modifications, equivalents, improvements and the like made to the above embodiments in accordance with the technical spirit of the present invention should be included in the scope of the present invention.
Claims (10)
1. An animal-specific EPI diagnostic reagent, which is characterized by consisting of a first diagnostic reagent, a second diagnostic reagent and a third diagnostic reagent, wherein the first diagnostic reagent can be matched with the second diagnostic reagent or the third diagnostic reagent for use;
the first diagnostic reagent comprises the following components in parts by weight: 0.85-5.5 parts of sodium chloride or glucose and 100 parts of ultrapure water;
the second diagnostic reagent comprises the following components in parts by weight: 1-5 parts of iodine, 2-10 parts of potassium iodide and 85-97 parts of ultrapure water;
the third diagnostic reagent comprises the following components in parts by weight: 1-2 parts of Sudan dye, 50 parts of 70% ethanol and 50 parts of acetone.
2. The animal-specific EPI diagnostic reagent according to claim 1, wherein the first diagnostic reagent comprises the following components in parts by weight: 0.85-0.95 parts of sodium chloride and 100 parts of ultrapure water.
3. The animal-specific EPI diagnostic reagent according to claim 1, wherein the first diagnostic reagent comprises the following components in parts by weight: 4.5-5.5 parts of glucose and 100 parts of ultrapure water.
4. The animal specific EPI diagnostic reagent of claim 1, wherein the Sudan dye is Sudan III dye or Sudan IV dye.
5. The animal-specific EPI diagnostic reagent according to claim 2, wherein the first diagnostic reagent comprises the following components in parts by weight: 0.9 part of sodium chloride and 100 parts of ultrapure water.
6. The animal-specific EPI diagnostic reagent according to claim 3, wherein the first diagnostic reagent comprises the following components in parts by weight: 5 parts of glucose and 100 parts of ultrapure water.
7. The animal-specific EPI diagnostic reagent according to claim 4, wherein the third diagnostic reagent comprises 1.5 parts of Sudan III dye.
8. The animal-specific EPI diagnostic reagent according to any one of claims 1 to 7, wherein the second diagnostic reagent comprises the following components in parts by weight: iodine 1.67 parts, potassium iodide 3.33 parts and ultrapure water 95 parts.
9. The animal-specific EPI diagnostic reagent according to claim 8, wherein the first diagnostic reagent further comprises the following components in parts by weight: 0.005-0.015 part of preservative.
10. The animal specific EPI diagnostic reagent of claim 9, wherein said preservative is thimerosal sodium.
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| CN202010269182.8A CN111487240A (en) | 2020-04-08 | 2020-04-08 | Special EPI diagnostic reagent for animals |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010269182.8A CN111487240A (en) | 2020-04-08 | 2020-04-08 | Special EPI diagnostic reagent for animals |
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2020
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| US4859604A (en) * | 1987-08-27 | 1989-08-22 | Ampor, Inc. | Composition for stabilization of diagnostic reagents |
| CN1381728A (en) * | 2001-04-20 | 2002-11-27 | 呼和浩特同日试剂有限公司 | Process for preparing diagnosing reagent kit of amylase and isozyme |
| CN104198474A (en) * | 2014-08-14 | 2014-12-10 | 上海睿康生物科技有限公司 | Detection kit for measuring content of lipase in serum by colorimetric method |
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| Title |
|---|
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