CN111349558B

CN111349558B - Lattice-shaped cell guiding device with controllable magnetic induction intensity

Info

Publication number: CN111349558B
Application number: CN202010080692.0A
Authority: CN
Inventors: 李兴富
Original assignee: Chongqing Technology and Business University
Current assignee: Chongqing Technology and Business University
Priority date: 2020-02-05
Filing date: 2020-02-05
Publication date: 2023-06-06
Anticipated expiration: 2040-02-05
Also published as: CN111349558A

Abstract

The invention discloses a lattice-shaped cell guiding device with controllable magnetic induction intensity, which is characterized in that limiting holes distributed in an array form are formed in a fixed module, miniature power-on solenoids are arranged in the limiting holes, each limiting hole is internally provided with a miniature power-on solenoid, the center of each miniature power-on solenoid is provided with a soft magnetic iron wire, and the miniature power-on solenoid is wound on the periphery of the soft magnetic iron wire to form an electromagnet; the power-on lead led out from the bottom of each micro power-on solenoid is independently connected with the controller, the power-on state of each micro power-on solenoid is controlled by the controller, and the magnetization and demagnetization of the soft magnet wire are realized by changing the magnetic field state generated by each micro power-on solenoid; each micro-electrified solenoid can generate an independent magnetic field, a plurality of micro-electrified solenoids distributed in an array form a locally controllable variable magnetic field, the accurate and flexible control of the magnetic induction intensity of the surface of each soft magnet wire is realized through magnetic field superposition, and the ordered position distribution of cells is accurately guided through a magnetic microcarrier.

Description

Lattice-shaped cell guiding device with controllable magnetic induction intensity

Technical Field

The invention relates to the technical field of tissue engineering cell assembly, in particular to a lattice-shaped cell guiding device with controllable magnetic induction intensity.

Background

Various unexpected natural disasters and behavioral accidents easily cause damage to tissues and organ loss of human bodies, and aging and degradation of tissues and organs also threaten human health. Tissue engineering develops an ordered structure with the functions of human organs through three-dimensional assembly of cells, and provides a new way for organ repair and regeneration. The human tissue is an ordered structure composed of a plurality of cells, and how to realize the precise spatial arrangement of different cells is a difficult problem faced by the development of the current cell three-dimensional assembly technology.

The cell microcarrier wraps the cells through biological materials, and provides basic life activity places for the cells, so that the survival rate is improved. The construction of artificial tissue scaffolds by manipulating cell microcarriers is more efficient than direct three-dimensional assembly of cells, and can also reduce mechanical damage to cells. Three key conditions are required for cell microcarrier operation: first, soft and sufficient assembly effort; second, effective spatial position control; thirdly, simulating an in-situ environment to perform micro-operation.

The micro-operation robot system can bring the accuracy, flexibility and high efficiency of robot operation into the three-dimensional assembly of the cell microcarrier, but the conventional micro-operation method needs complex peripheral equipment and higher manufacturing cost, and has the problems of direct contact and the like. The three-dimensional assembly of the robot micromanipulation in the air has certain control precision, but the spatial position of the cell microcarrier is difficult to accurately control under the influence of fluid disturbance in a liquid environment.

The cell microcarrier has the characteristics of small size, softness, easy deformation, poor controllability and the like, is difficult to be effectively arranged at a desired position in space, and the addition of the magnetic nano particles into the cell microcarrier has small influence on cells, so that the response characteristic to an external magnetic field can be improved, and the controllability of the cells is improved.

In the process of operating the cell microcarrier by using the conventional permanent magnet in a liquid environment, the problems that the intensity of the magnetic field is difficult to change, the generation and elimination of the magnetic field are inflexible and the like exist, and the permanent magnet with the nonuniform magnetic field can gather the magnetic microcarrier to the surface edge and make the magnetic microcarrier difficult to arrange orderly, so that the construction of a complex artificial tissue scaffold is not facilitated.

How to precisely and flexibly control the spatial distribution of the magnetic field is a technical problem to be solved at present for those skilled in the art.

Disclosure of Invention

The invention provides a lattice-shaped cell guiding device with controllable magnetic induction intensity, which can accurately and flexibly control the space distribution state of a magnetic field, and the specific scheme is as follows:

the utility model provides a controllable cell guiding device of lattice form magnetic induction intensity, includes fixed module, set up the spacing hole of a plurality of array form distributions on the fixed module, be used for assembling spacing miniature circular telegram solenoid in the spacing hole, every miniature circular telegram solenoid's center sets up soft magnetism iron wire, every circular telegram wire that miniature circular telegram solenoid's bottom was drawn forth is independent to be connected in the controller.

Optionally, the spacing hole is the through-hole, the bottom of fixed module sets up the wire piece, set up the wire passageway on the wire piece, the circular telegram wire clamps in the wire passageway.

Optionally, the wire block is formed by fixing a plurality of wire guide plates which are stacked; the miniature energizing solenoids are arranged in a multi-circle surrounding manner, and energizing wires led out by each circle of miniature energizing solenoids are clamped on one wire guide plate;

the energizing wires are radially distributed and led out from the edge of the wire guide plate.

Optionally, the energizing wires led out from the miniature energizing solenoids of each turn from the outer turn to the inner turn are correspondingly clamped on each wire guide plate distributed from top to bottom in sequence.

Optionally, the micro-energized solenoids are distributed in a rectangular array.

Optionally, the securing module and the wire block are made of Polydimethylsiloxane (PDMS).

The invention provides a lattice-shaped cell guiding device with controllable magnetic induction intensity, which comprises a fixed module, wherein a plurality of limit holes distributed in an array form are formed in the fixed module, the limit holes are deep holes, miniature power-on solenoids can be installed in the limit holes in a limited mode, each limit hole is internally provided with a miniature power-on solenoid, each miniature power-on solenoid is in a spiral winding wire structure, the center of each miniature power-on solenoid is provided with a soft magnetic iron wire, and the miniature power-on solenoid is wound on the periphery of the soft magnetic iron wire to form an electromagnet; the bottom of each micro-electrifying solenoid is led out of an electrifying wire, each electrifying wire is independently connected with a controller, the electrifying state of each micro-electrifying solenoid is controlled by the controller, and the magnetization and demagnetization of the soft magnet wire are realized by changing the magnetic field state generated by each micro-electrifying solenoid; because each micro-electrified solenoid can generate an independent magnetic field, a plurality of micro-electrified solenoids distributed in an array form a locally controllable changing magnetic field, thereby realizing the precise and flexible control of the magnetic induction intensity of the surface of each soft magnet wire through magnetic field superposition and further precisely guiding the ordered position distribution of cells through the magnetic microcarrier.

Drawings

In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is an isometric view of the appearance of a lattice-shaped cell guidance device with controllable magnetic induction;

FIGS. 2A and 2B are an isometric view and a top view, respectively, of the opposite connection of the outermost miniature energized solenoid to the wire guide plate;

FIGS. 3A and 3B are an isometric view and a top view, respectively, of the secondary outer ring micro-energized solenoid in opposing connection with the wire guide plate;

FIGS. 4A and 4B are an isometric view and a top view, respectively, of a third turn of a micro-energized solenoid from the outside toward the inside, in opposing connection with a wire guide plate;

FIGS. 5A and 5B are an isometric view and a top view, respectively, of the opposing connection of the secondary inner ring micro-energized solenoid to the wire guide plate;

fig. 6A and 6B are an isometric view and a plan view, respectively, of the opposite connection of the innermost miniature energized solenoid to the wire guide plate.

The drawings include:

the device comprises a fixed module 1, a limiting hole 11, a miniature power-on solenoid 2, a soft magnetic iron wire 21, a power-on wire 22 and a wire block 3.

Detailed Description

The invention aims at providing a lattice-shaped cell guiding device with controllable magnetic induction intensity, which can accurately and flexibly control the space distribution state of a magnetic field.

In order to make the technical solution of the present invention better understood by those skilled in the art, the lattice-shaped magnetic induction controllable cell guiding device of the present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

As shown in fig. 1, an isometric view of an appearance structure of the lattice-shaped magnetic induction intensity controllable cell guiding device provided by the invention is shown; the device comprises a fixed module 1, wherein the fixed module 1 is of a limit bearing structure, a plurality of limit holes 11 distributed in an array form are formed in the fixed module 1, the limit holes 11 can be through holes or blind holes, the limit columns 11 are of a slender columnar structure, a limit miniature electrifying solenoid 2 is assembled in the limit holes 11, the miniature electrifying solenoid 2 is spirally wound on the periphery of a soft magnetic iron wire 21 to form an electromagnet, and two poles of the electromagnet are respectively arranged along the length direction of the soft magnetic iron wire 21; the bottom of each micro-energizing solenoid 2 is led out of an energizing wire 22, the bottom of which refers to the direction shown in the figure, the energizing wire 22 extends out of the fixed module 1, each energizing wire 22 is independently connected with a controller, and the energizing state of each micro-energizing solenoid 2 is independently controlled by the controller, so that the magnetization and demagnetization of the soft magnetic iron wires are realized.

The magnetic field is formed independently after each micro-energizing solenoid 2 is energized, the energizing state of each micro-energizing solenoid 2 can be controlled independently, and the controller adjusts the current of each micro-energizing solenoid 2, so that the magnetization and demagnetization of the soft magnetic iron wires 21 are realized. The strength of the magnetic induction intensity of the surface of each soft magnetic iron wire 21 is flexibly changed through magnetic field superposition, and the generation and elimination of the magnetic field can be flexibly controlled, so that the spatial position of the magnetic microcarrier is accurately and flexibly controlled, and the ordered position distribution of cells is accurately guided; when in use, the generation and elimination of the magnetic induction intensity and the strength of the surface of each soft magnetic iron wire 21 are controlled, so that the magnetic cell microcarrier moves to a designated position according to a designed route under the action of a magnetic field.

On the basis of the scheme, the limiting holes 11 are through holes, and the limiting holes 11 penetrate through the upper surface and the lower surface of the fixed module 1; the bottom of the fixed module 1 is provided with a wire block 3, the wire block 3 is used for bearing the fixed module 1, and the fixed module 1 and the wire block 3 are relatively and fixedly arranged; the wire block 3 is provided with a wire channel, the energizing wires 22 are clamped in the wire channel, the wire channel is used for guiding the energizing wires 22, the energizing wires 22 are led out of the fixed module 1 through the wire channel and then connected to the controller, the wire block 3 provides guidance for the energizing wires 22, so that the energizing wires 22 led out by the miniature energizing solenoids 2 are regularly distributed and do not interfere with each other.

Further, the conductor block 3 in the present invention is constituted by laminating and fixing a plurality of conductor plates each having a thickness larger than the diameter of the energizing conductor 22, grooves for guiding the energizing conductor 22 are provided in the plate surface direction on the respective conductor plates, and the respective energizing conductors 22 extend in the plate surface direction of the conductor plates.

The miniature power-on solenoids 2 are arranged in a multi-circle surrounding manner, corresponding limit holes 11 on the fixed module 1 are also arranged in a multi-circle surrounding manner, the miniature power-on solenoids 2 are arranged in a rectangular surrounding manner, a rectangular array structure is formed by surrounding a plurality of miniature power-on solenoids 2, each circle of rectangles are concentrically arranged, the miniature power-on solenoids 2 positioned on the same rectangle are one circle, and the size of each circle is gradually reduced from outside to inside; the micro-energizing solenoids 2 may be arranged concentrically, or may not be arranged concentrically, in addition to the rectangular concentric arrangement.

The number of turns of the miniature energizing solenoid 2 is equal to the number of layers of the wire guide plates, and energizing wires 22 led out by each turn of miniature energizing solenoid 2 are clamped on one wire guide plate; the energizing conductors 22 are radially distributed from the edge of the conductor plate, and each energizing conductor 22 is led out of the conductor plate along a relatively short path.

The energizing wires 22 led out from each turn of the micro energizing solenoid 2 from the outer ring to the inner ring are correspondingly clamped on each wire guide plate distributed from top to bottom in sequence, namely, the energizing wires 22 led out from the micro energizing solenoid 2 at the outermost ring are guided by the wire guide plate at the uppermost layer, the energizing wires 22 led out from the micro energizing solenoid 2 at the innermost layer are guided by the wire guide plate at the lowermost layer, and the energizing wires 22 led out from the micro energizing solenoids 2 at other turns are sequentially guided by the wire guide plates at each layer.

As shown in fig. 2A and 2B, which are respectively an isometric view and a plan view of the connection of the outermost miniature energized solenoid 2 and the wire guide plate, the energized wires 22 of the outermost miniature energized solenoid are assembled in cooperation with the uppermost wire guide plate, and the energized wires 22 extend along the wire guide channels formed in the uppermost wire guide plate.

As shown in fig. 3A and 3B, which are respectively an isometric view and a plan view of the connection of the secondary outer ring micro-energizing solenoid 2 and the wire guide plate, the energizing wires 22 of the secondary outer ring are assembled in a matched manner with the wire guide plate of the second layer from top to bottom, and the energizing wires 22 led out from the secondary outer ring micro-energizing solenoid 2 pass through the holes on the wire guide plate of the uppermost layer from top to bottom and then extend along the wire guide channels formed by the wire guide plate of the second layer.

As shown in fig. 4A and 4B, the third turn of micro-energizing solenoid 2 is connected to the wire guide plate from outside to inside in an isometric view and a plan view, respectively, and the energizing wires 22 led out from the third turn of micro-energizing solenoid 2 first pass through the openings on the uppermost wire guide plate and the second wire guide plate from top to bottom, and then extend along the wire guide channel formed on the third wire guide plate.

As shown in fig. 5A and 5B, which are respectively an isometric view and a plan view of the connection of the secondary inner ring micro-energized solenoid 2 and the wire guide plate, the energized wires 22 led out from the secondary inner ring micro-energized solenoid 2 first pass through the openings on the uppermost layer, the second layer and the third layer of wire guide plate from top to bottom, and then extend along the wire guide channel formed on the fourth layer of wire guide plate.

As shown in fig. 6A and 6B, which are respectively an isometric view and a plan view of the connection of the innermost micro-energized solenoid 2 and the wire guide plate, the energized wires 22 led out from the innermost micro-energized solenoid 2 first pass through the openings in the uppermost layer, the second layer, the third layer and the fourth layer of wire guide plate from top to bottom, and then extend along the wire guide channels formed in the lowermost layer of wire guide plate.

The above figures illustrate a configuration in which five turns of the micro-energizing solenoid 2 and five layers of wire guide plates are provided, and more or fewer turns of the micro-energizing solenoid and the number of layers of wire guide plates may be provided if desired. The invention guides the energizing wires 22 through the wire plates which are arranged in a laminated way, avoids mutual interference among the energizing wires 22 of each circle, and solves the difficult problem that the structure of the device with complicated energizing input and output wiring of the conventional miniature coil occupies a large space and is difficult to miniaturize.

Specifically, the fixing module 1 and the wire block 3 are made of Polydimethylsiloxane (PDMS) in the present invention.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. The lattice-shaped cell guiding device with controllable magnetic induction intensity is characterized by comprising a fixing module (1), wherein a plurality of limit holes (11) distributed in an array shape are formed in the fixing module (1), limiting miniature power-on solenoids (2) are assembled in the limit holes (11), soft magnetic iron wires (21) are arranged at the center of each miniature power-on solenoid (2), and power-on wires (22) led out from the bottom of each miniature power-on solenoid (2) are independently connected to a controller;

the limiting hole (11) is a through hole, a wire block (3) is arranged at the bottom of the fixing module (1), a wire channel is arranged on the wire block (3), and the energizing wire (22) is clamped in the wire channel;

the wire guide block (3) is formed by fixing a plurality of wire guide plates which are arranged in a stacked manner; the miniature power-on solenoid (2) is arranged in a multi-circle surrounding manner, the number of turns of the miniature power-on solenoid (2) is equal to the number of layers of the wire guide plate, and each circle of power-on wires (22) led out by the miniature power-on solenoid (2) are clamped on one wire guide plate;

the energizing wires (22) are radially distributed and led out from the edge of the wire guide plate.

2. The lattice-like magnetic induction controllable cell guiding device according to claim 1, wherein the energizing wires (22) led out from the micro energizing solenoids (2) of each turn from the outer turn to the inner turn are respectively clamped on the wire guide plates distributed from top to bottom.

3. The lattice-like magnetic induction controllable cell guiding device according to claim 1, wherein the micro-energized solenoids (2) are distributed in a rectangular array.

4. The lattice-like magnetic induction controllable cell guiding device according to claim 1, characterized in that the fixing module (1) and the wire block (3) are made of Polydimethylsiloxane (PDMS).