CN1111635A - Sphingomyelin extracting and application in cosmetics and foods - Google Patents
Sphingomyelin extracting and application in cosmetics and foods Download PDFInfo
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- CN1111635A CN1111635A CN94111318A CN94111318A CN1111635A CN 1111635 A CN1111635 A CN 1111635A CN 94111318 A CN94111318 A CN 94111318A CN 94111318 A CN94111318 A CN 94111318A CN 1111635 A CN1111635 A CN 1111635A
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- sheath
- phosphatide
- nerve
- liposome
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Abstract
The sphingomyelin capable of using in cosmetics and food can be obtained from the following processes: crushing the animal's viscera, extracting the cholesterol with acetone, extracting the sphingomyelin (containing lecithin and albumen) with hot alcohol, removing the lecithin by ether and washing out the albumen with acetone. Through the silica gel bilaterial thin-layer chromatography and phosphorous quantity test, the content of cholesterol is less than 3%, and moisture is less than 1%.
Description
The present invention relates to the extraction of nerve (sheath) phospholipid (Sphingomyelins) and make liposome (Liposomes) film material be used for daily cosmetics and foodstuff additive with neural (sheath) phosphatide.
Neural (sheath) phospholipid is non-glycerine phosphatide, and widely distributed in vivo, function is important, but content is lower.The report (1961) that extracts neural (sheath) phosphatide from red corpuscle was once arranged, but the techniqueflow complexity yields poorly, the cost height is only as biochemical reagents.（Hanahan D.J.，Biochem.Prep 1961;8：121）
Hanahan method of reporting main contents are: anti-freezing ox blood 10L, and 4 ℃ of following centrifuging and taking red blood corpuscle, physiological saline is centrifugal again after cleaning, and gets red blood corpuscle 3.9L.The gained red corpuscle adds 0.25 volume distilled water mixed room temperature and leaves standstill 3～5 ', add 3 volumes, 95% ethanol, stir the back room temperature and place 2h.After adding the stirring of 1 volume diethyl ether, put 4h, cross leaching filtrate and preserve (4 ℃), repeat above-mentioned steps, merging filtrate, after organic solvent is removed in the placement decompression, chromatography column separates, collecting precipitation thing behind the elutriant deepfreeze, vacuum-drying, get 2.2g nerve (sheath) phosphatide, wherein lecithin content is not more than 5%.Output/raw material is 0.022%, uses ethanol 13L, diethyl ether 5L and other medicine in the production.
The objective of the invention is to avoid above-mentioned weak point of the prior art and provide a kind of, and technology is simple, cost is low, output is high, can be widely used among makeup and the food with the extraction raw material of animal viscera as neural (sheath) phosphatide.
Purpose of the present invention can reach by following measure:
1, rationale:
A, animal viscera contain nerve (sheath) phosphatide of higher proportion, and for example: aorta 38.1%(accounts for the per-cent of lipid, down together), brain 5.6%, peripheral nerve 30.2%, lung 11.1%.
B, different lipid different solubility in different organic solvents.
The acetone ethanol ether
Cholesterol is broad molten
Kephalin is insoluble not broad
Yelkin TTS is insoluble broad
Neural (sheath) phosphatide not broad (heat) is insoluble
2, technical process,
Internal organs are pulverized, acetone extracting cholesterol, hot ethanol extracting nerve (sheath) phosphatide (containing Yelkin TTS and soluble protein), Yelkin TTS is removed in the ether extracting, washing with acetone removes Deproteinization, must can be used for nerve (sheath) phosphatide (can get cholesterol, Yelkin TTS simultaneously) of makeup and food.
As use the induced labor foetus internal organs to be raw material, can get humanized's nerve (sheath) phosphatide.
The present invention has following advantage compared to existing technology:
Now the phosphatide as the liposome coating materials is mainly Yelkin TTS and kephalin.The shortcoming of their maximums is a unstable, and deterioration by oxidation very easily under the normal temperature loses effect in air.Compare with Yelkin TTS, kephalin, neural (sheath) phosphatide not only in air, stable under the illumination, and contain matter important component ceramide between skin.So the liposome that makes with it adds in the makeup, obtains peculiar effect.In addition, because its stable and many-sided function also will come into one's own as food liposome additive.
This product is nontoxicity after testing, nonirritant, and no supersensitivity, all indexs of health meet the provisions of the relevant regulations issued by the State.
With the liposome that neural (sheath) phosphatide is made, particle carefully less, be evenly distributed, it is spherical that form mostly is, minority is thruster shape (electromicroscopic photograph image analysis, the average 124.25 ± 44.33nm of diameter, (50～300nm)).
Crowd's experimental result was good after simple nerve (sheath) the phosphatide plastid of making and nerve (sheath) phosphatide-snake oil liposome added makeup to.Addition 10
-4The time, skin is moist, comfortable.Once a day, attenuate tender, flexible, wrinkle of a week back skin reduces.The skin chloasma is thin out after January.While is not sticking, oiliness, air permeability is good.
Embodiment 1,
Pig brain homogenate 200g+ acetone 300ml stirs 4h, filters.
Filter cake adds the qdx dehydrated alcohol, and 60 ℃ water-soluble.Stir 4h, filter repetitive operation 3 times.
Merging filtrate is separated out precipitation under-15 ℃.Cold filtration, drying under reduced pressure gets crude product.
Crude product adds the qdx ether, cap seal in Erlenmeyer flask or beaker, and vibration 4h, taking precipitate repeats the ether washing once.
Ether sedimentation thing washing with acetone 2 times, lyophilize gets neural (sheath) phosphatidase 10 .8g.
Embodiment 2,
Cattle sciatic nerve homogenate 100g+ acetone 200ml stirs 4h, filters.
Following steps with embodiment 1,
Get neural (sheath) phosphatidase 12 .5g.
Gained nerve (sheath) phosphatide is through two-way thin-layer chromatography of silica gel and phosphorus quantitative testing, neural (sheath) phospholipids content 〉=96%, cholesterol≤3%, moisture content＜1%.
Embodiment 3, liposome-snake gall frost
Stearic acid 12%, Solsperse 2000 8%, lanolin 4%, glycerine 10%, spices 1%, sanitas is an amount of, and smart water adds to 100%, phosphatidic acid 1.5%, cholesterol 0.5%, neural (sheath) phosphatidase 10 .01%, snake gall 0.1%.
Phosphatidic acid, cholesterol, nerve (sheath) phosphatide, snake gall are made liposome, rest part is made lotion, then thorough mixing.
Liposome-snake gall frost is specially adapted to oily skin.
Embodiment 4, liposome oral fluid
Marine alga concentrated solution 3%, soybean extract 3%, white sugar 1%, honey 1%, spices is an amount of, and sanitas is an amount of, NaHCO
30.2%, cerebrolysin 0.1%, Vitc0.1%, neural (sheath) phosphatidase 10 .01%, phosphatidic acid 1%, smart water adds to 100%.
With cerebrolysin, VitC, neural (sheath) phosphatide, phosphatidic acid is prepared into liposome, is made into liposome oral fluid jointly with other compositions then.
This kind oral liquid activeconstituents is subjected to the phosphatide protection, has obvious brain-invigorating.
Claims (3)
1, the extraction process of a kind of nerve (sheath) phosphatide and in makeup and Application in Food, it is characterized in that animal viscera is pulverized, acetone extracting cholesterol, hot ethanol extracting nerve (sheath) phosphatide (containing Yelkin TTS and soluble protein), Yelkin TTS is removed in the ether extracting, washing with acetone removes Deproteinization, must can be used for nerve (sheath) phosphatide of makeup and food.
2, the application of nerve according to claim 1 (sheath) phosphatide in makeup, it is characterized in that phosphatidic acid, cholesterol, nerve (sheath) phosphatide, snake gall are made liposome, stearic acid, Solsperse 2000, lanolin, glycerine, spices, sanitas are made lotion, then thorough mixing liposome-snake gall frost.
3, nerve according to claim 1 (sheath) phosphatide is in Application in Food, it is characterized in that cerebrolysin, Vitc, nerve (sheath) phosphatide, phosphatidic acid are prepared into liposome, then with marine alga concentrated solution, soybean extract, white sugar, honey, spices, sanitas, NaHCO
3Be made into liposome oral fluid jointly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94111318A CN1111635A (en) | 1994-05-10 | 1994-05-10 | Sphingomyelin extracting and application in cosmetics and foods |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94111318A CN1111635A (en) | 1994-05-10 | 1994-05-10 | Sphingomyelin extracting and application in cosmetics and foods |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1111635A true CN1111635A (en) | 1995-11-15 |
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ID=5035201
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN94111318A Pending CN1111635A (en) | 1994-05-10 | 1994-05-10 | Sphingomyelin extracting and application in cosmetics and foods |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1111635A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0887070A1 (en) * | 1997-05-30 | 1998-12-30 | Kibun Food ChemiFA Co., Ltd. | External composition for skin comprising sphingoglycolipid |
| CN101272793B (en) * | 2005-09-22 | 2011-02-09 | 雪印乳业株式会社 | Medicine, food, drink, or feed containing sphingomyelin |
| CN1997376B (en) * | 2004-03-30 | 2011-05-11 | 雪印乳业株式会社 | skin-beautifying agent |
-
1994
- 1994-05-10 CN CN94111318A patent/CN1111635A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0887070A1 (en) * | 1997-05-30 | 1998-12-30 | Kibun Food ChemiFA Co., Ltd. | External composition for skin comprising sphingoglycolipid |
| CN1997376B (en) * | 2004-03-30 | 2011-05-11 | 雪印乳业株式会社 | skin-beautifying agent |
| CN101272793B (en) * | 2005-09-22 | 2011-02-09 | 雪印乳业株式会社 | Medicine, food, drink, or feed containing sphingomyelin |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |