CN110935274A

CN110935274A - Air filtering device for reducing bacterial contamination of amino acid fermentation

Info

Publication number: CN110935274A
Application number: CN201911288340.8A
Authority: CN
Inventors: 王小平; 曹博超; 董吉子; 尤学波
Original assignee: Zhao Lankun
Current assignee: Zhao Lankun
Priority date: 2019-09-18
Filing date: 2019-12-16
Publication date: 2020-03-31

Abstract

The invention discloses an air filtering device for reducing amino acid fermentation contamination, which comprises an air inlet pipe; a filter box, an air compressor, an air storage tank, a cooling tank, a cyclone separator, a dehumidifier, a heating tank, a cooling tank, a main air filter and a sub-air filter are sequentially arranged behind the air inlet pipe, and clean air enters the sterile air storage tank to be stored after passing through the sub-air filter; the sterile air storage tank is connected with the fermentation equipment. The invention has simple structure, adopts multi-layer filtration and sterilization, and the filter elements can be replaced at any time by connecting a plurality of groups in parallel, thereby effectively solving the technical problems of poor filtration effect and easy contamination of bacteria in the air system in the amino acid fermentation in the prior art.

Description

Air filtering device for reducing bacterial contamination of amino acid fermentation

Technical Field

The invention belongs to the technical field of fermentation equipment, and particularly relates to an air filtering device for reducing amino acid fermentation contamination.

Background

In the production of amino acid fermentation industry, the production process is mostly a pure culture process, and an important condition for smooth fermentation production is to ensure the pure culture of microorganisms and to perform the fermentation production under the condition of no mixed bacteria pollution. In actual production, fermentation and bacterial contamination are often caused due to reasons such as unreasonable process design, equipment defects, personnel errors and the like. Since the fermentation production with the mixed bacteria consumes the nutrient substances in the culture medium, the metabolite of the mixed bacteria even influences the growth and metabolism of the producing bacteria, and the fermentation yield is reduced. Once the contamination occurs, the cause of the contamination should be found as soon as possible and corresponding effective measures should be taken to minimize the loss caused by the contamination of fermentation. The glutamic acid fermentation phage contamination rate is reported to be 1% -2%. Therefore, the reduction of the contamination rate is the key point of the fermentation production and is an important means for improving the yield of new products and reducing the product cost of enterprises.

In the fermentative production of amino acids, the so-called infectious bacteria include two types: one is the staining of phage, which is extremely harmful to production, and if large-scale outbreaks occur, the plant is forced to stop production. Although the bacteriophage is extremely harmful, as long as various work of the bacteriophage is prevented from being implemented in place, the infection of the bacteriophage can be basically avoided in the fermentation process, and the other is infectious microbes which are thalli except amino acid producing bacteria. Meanwhile, the invasion of the mixed bacteria is often a precursor of the pollution of the bacteriophage in the pure culture process, so the idea of preventing the bacteria from being infected mainly needs to be established, the management work in production is enhanced, and the bacteria-infecting factors are eliminated in the bud. The contamination reasons in the fermentation process mainly focus on the following aspects: air system, environmental factors, equipment factors, operational factors, and seed factors.

The air system is the main reason for causing the fungus contamination in the fermentation process, and the air is filtered and sterilized before entering the fermentation equipment, wherein the PP cotton filter element is adopted for filtering, dust and impurities in the air are filtered, and an ultraviolet spot lamp, a filter and the like are adopted for sterilization. Although most of fungi can be filtered, the air is not clean, a small amount of fungi spores and other impurities still exist, the fungi and the impurities enter the fermentation tank, required oxygen and environment are obtained, the fungi can grow rapidly, particularly, the growth and the division of the fungi are extremely fast, and the product quality is seriously influenced. And the filter core has been used for a long time and can be the saturation state, need stop the disconnection with equipment is whole when changing, and it is very inconvenient to use.

Disclosure of Invention

The invention aims to solve the technical problems of poor filtering effect and easy bacterial contamination of an air system in amino acid fermentation in the prior art, and provides an air filtering device for reducing bacterial contamination in amino acid fermentation.

In order to achieve the purpose, the invention adopts the following technical scheme:

an air filtering device for reducing the bacterial contamination of amino acid fermentation comprises an air inlet pipe; a filter box, an air compressor, an air storage tank, a cooling tank, a cyclone separator, a dehumidifier, a heating tank, a cooling tank, a main air filter and a sub-air filter are sequentially arranged behind the air inlet pipe, and clean air enters the sterile air storage tank to be stored after passing through the sub-air filter; the sterile air storage tank is connected with the fermentation equipment.

Furthermore, at least two branch pipes are arranged behind the air inlet pipe; the filter box is arranged on the branch pipe; the branch pipe behind the filter box is connected with the air compressor through a communicating pipe; and control valves are respectively arranged on the branch pipes corresponding to the two ends of the filter box.

Furthermore, a plurality of vertically distributed partition plates are arranged in the filter box; filter elements which are independently distributed are arranged in a cavity formed between the partition plates; vent holes are reserved between the partition plates and the filter box, and the vent holes of the adjacent partition plates are distributed in a staggered manner; a cover plate is arranged on the filter box; a lock pin is arranged between the cover plate and the filter box; the periphery of the cover plate corresponding to the filter box and the corresponding part of the partition plate are provided with sealing gaskets.

Further, a pressure gauge and a pressure regulating valve are arranged on the air storage tank; and a one-way valve is arranged on a communicating pipe between the air storage tank and the air compressor.

Furthermore, two hollow air discs which are distributed in parallel are arranged in the cooling box; the air discs are connected and communicated through branch pipes; the communicating pipe extends into the cooling box and is respectively connected with the rear sides of the two hollow air discs; and a cooling water circulation interface is arranged on the cooling box.

Further, a silk screen layer is arranged in the dehumidifier.

Furthermore, the heating box adopts electric heating, and a heating wire is wound on a pipeline through which air flows.

Furthermore, the air main filter is divided into two chambers which are symmetrically distributed by a middle partition plate, and two ends of each chamber are respectively connected with the communicating pipe through branch pipes with control valves; a filter cylinder and a corresponding sterilizing tube are arranged in the chamber; the communicating pipe at the air inlet end enters two chambers of the air main filter through the branch pipe, and the branch pipe is connected with the filter cartridge through the flexible joint pipe; a stop block for fixing the filter cartridge is arranged in the chamber.

Furthermore, the air branch filters are provided with a plurality of groups which are mutually distributed in parallel through branch pipes; and a sub-filter membrane is arranged in the air sub-filter.

The invention has simple structure, adopts multi-layer filtration and sterilization, and the filter elements can be replaced at any time by connecting a plurality of groups in parallel, thereby effectively solving the technical problems of poor filtration effect and easy contamination of bacteria in the air system in the amino acid fermentation in the prior art.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the filter cassette of the present invention;

FIG. 3 is a schematic view of the cooling box structure of the present invention;

fig. 4 is a schematic top view of fig. 3.

In figure 1, an air inlet pipe; 2. a branch pipe; 3. a control valve; 4. a filter cartridge; 5. a partition plate; 6. a filter element; 7. a communicating pipe; 8. an air compressor; 9. a one-way valve; 10. an air reservoir; 11. a pressure gauge; 12. a pressure regulating valve; 13. a cooling tank; 14. pipe distribution; 15. a circulation interface; 16. a cyclone separator; 17. a dehumidifier; 18. a silk screen layer; 19. a heating box; 20. heating wires; 21. a flexible joint pipe; 22. a main air filter; 23. a sterilizing tube; 24. a middle partition plate; 25. a filter cartridge; 26. an air separation filter; 27. separating filter membranes; 28. a sterile air storage tank; 29. a cover plate; 30. a lock pin; 31. a gasket; 32. an air disk.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it is apparent that the embodiments described below are some, but not all embodiments of the present invention. All other embodiments which can be obtained by a person skilled in the art without any inventive work based on the embodiments of the present invention belong to the protection scope of the present invention, and it should be noted that words indicating the orientation in the text, such as upper and lower, left and right, etc., are expressed based on the description given in the drawings, not specific limitations to the components.

Example 1: referring to fig. 1, 2, 3 and 4, an air filter device for reducing contamination of amino acid fermentation includes an air inlet pipe 1; the rear part of the air inlet pipe is sequentially provided with a filter box 4, an air compressor 8, an air storage tank 10, a cooling tank 13, a cyclone separator 16, a dehumidifier 17, a heating tank 19, a cooling tank 13, an air main filter 22 and an air sub-filter 26, and clean air enters a sterile air storage tank 28 to be stored after passing through the air sub-filter; the sterile air storage tank is connected with the fermentation equipment.

Air enters the filter box 4 through the air inlet pipe 1, is subjected to primary filtration, then enters the air storage tank 10 through the air compressor 8 and is stored; the temperature of the compressed air rises to a certain extent, the compressed air is cooled by the cooling box 13 and then is dedusted by the cyclone separator 16, and fine dust impurities are separated; then the moisture is removed in the dehumidifier 17; heated to sterilize in the heating box 19, then cooled again, passed through the main air filter 22 and the sub air filter 26 in sequence, and finally stored in the sterile air storage 28 for later use.

At least two branch pipes 2 are arranged behind the air inlet pipe 1; the filter box 4 is arranged on the branch pipe; the branch pipe behind the filter box is connected with the air compressor through a communicating pipe 7; and control valves 3 are respectively arranged on the branch pipes corresponding to the two ends of the filter box.

The two groups of filter boxes connected in parallel are arranged, so that the other group can be started under the stopping states of replacing the filter element, cleaning, adjusting and the like, the working efficiency is not influenced, sufficient time is brought to replacing and cleaning, and the work is more careful.

A plurality of vertically distributed partition plates 5 are arranged in the filter box 4; filter elements 6 which are independently distributed are arranged in a cavity formed between the partition plates; vent holes are reserved between the partition plates and the filter box, and the vent holes of the adjacent partition plates are distributed in a staggered manner; a cover plate 29 is arranged on the filter box; a lock pin 30 is arranged between the cover plate and the filter box; the periphery of the cover plate corresponding to the filter box and the corresponding part of the partition plate are provided with sealing gaskets 31.

The filter element 6 in the filter box 4 adopts a PP cotton filter element, and the density is gradually increased from one side of the air inlet pipe 1 to the other side.

A pressure gauge 11 and a pressure regulating valve 12 are arranged on the air storage tank 10; and a one-way valve 9 is arranged on a communicating pipe between the air storage tank and the air compressor.

Two hollow air discs 32 which are distributed in parallel are arranged in the cooling box 13; the air discs are connected and communicated through branch pipes 14; the communicating pipe extends into the cooling box and is respectively connected with the rear sides of the two hollow air discs; and a cooling water circulation interface 15 is arranged on the cooling box.

The branch pipes 14 are ceramic pipes, cooling water circulates in the cooling box 13 through the circulation connectors 15, and heat exchange is achieved through gaps of the branch pipes 14 for cooling.

Under the condition permission circumstances, can make more than 3m boxes of a volume with cooler bin 13, the box adopts open structure or sets up the fin structure all around, need not to set up circulation interface 15 like this, reduces a pump package, reduces the loss.

A silk screen layer 18 is arranged in the dehumidifier 17. The silk screen layer 18 is made of woven cloth, after air enters the cooling box 13, the temperature drops suddenly, moisture can be condensed into fine particles, and the fine particles are adsorbed after passing through the silk screen layer 18.

The heating box 19 is electrically heated, and a heating wire 20 is wound on a pipeline through which air flows.

The heating box 19 is used for high-temperature sterilization, the time for air to flow through the heating box 19 is 20-45 seconds, and the air flow pipeline in the heating box 19 can adopt a coil pipe to increase the path; the heating wire 20 controls the temperature of the air at 105-120 ℃, at which most of the fungi, bacteria, spores, etc. are killed.

The air main filter 22 is divided into two chambers which are symmetrically distributed by a middle partition plate 24, and two ends of each chamber are respectively connected with a communicating pipe through branch pipes with control valves; a filter cylinder 25 and a corresponding sterilizing tube 23 are arranged in the chamber; the communicating pipe at the air inlet end enters two chambers of the air main filter through the branch pipe, and the branch pipe is connected with the filter cartridge through a soft joint pipe 21; a stop block for fixing the filter cartridge is arranged in the chamber.

The main air filter 22 is divided into two symmetrically distributed chambers which can be used alternately, for the same reason as the filter cassette 4. The sterilizing tube 23 can be sterilized for the second time, and the chamber is contacted with the outside to be infected when the filter cartridge 25 is replaced, and is also sterilized through the sterilizing tube 23.

The filter cartridge 25 comprises a membrane body, a bracket and a front cover body; the bracket is used for supporting the membrane body; the front cover body is provided with a threaded hole corresponding to the soft joint pipe 21; the membrane body is a composite membrane and comprises a plurality of layers of polytetrafluoroethylene membrane layers and PET non-woven fabric layers which are alternately distributed; the membrane pores of the polytetrafluoroethylene membrane layer are 20-50 microns.

The filter cartridge 25 thoroughly filters impurities such as micro-dust, spores, bacteria and the like, so that air is clean.

The air filter 26 is provided with a plurality of groups which are distributed in parallel through branch pipes; the air sub-filter is internally provided with a sub-filter membrane 27.

The filtering membrane 27 is a multi-layer composite membrane, which comprises a polytetrafluoroethylene membrane layer, a PET non-woven fabric layer and a dispersed resin membrane, wherein the membrane pores are 20-35 microns and are formed by extruding a plurality of membranes.

Example 2, experimental comparison, the influence of the filter device and the common filter on the fermentation contamination, and other conditions are the same, as shown in the following table:

fermenting for 10h	Experimental group	Control group
			Mould fungus	0.03%	0.55%
Coccus bacterium	0	0.35%
			Spiral body	0	0.10%
Other bacterial cells	0.04%	0.70%

As can be seen from the table above, with the application, the probability of bacterial infection is extremely low, and only a small amount of mould and other thalli are infected; and the ordinary filter equipment can cause more than 1% of other bacterial infection, and the filter effect of the application is improved by about 20 times.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims

1. An air filtering device for reducing the bacterial contamination of amino acid fermentation comprises an air inlet pipe; the air purifier is characterized in that a filter box, an air compressor, an air storage tank, a cooling tank, a cyclone separator, a dehumidifier, a heating tank, a cooling tank, a main air filter and a sub-air filter are sequentially arranged behind the air inlet pipe, and clean air passes through the sub-air filter and then enters the sterile air storage tank for storage; the sterile air storage tank is connected with the fermentation equipment.

2. The air filtering device for reducing the contamination of amino acid fermentation bacteria according to claim 1, wherein: at least two branch pipes are arranged behind the air inlet pipe; the filter box is arranged on the branch pipe; the branch pipe behind the filter box is connected with the air compressor through a communicating pipe; and control valves are respectively arranged on the branch pipes corresponding to the two ends of the filter box.

3. The air filtering device for reducing the contamination of amino acid fermentation according to claim 1 or 2, wherein: a plurality of vertically distributed partition plates are arranged in the filter box; filter elements which are independently distributed are arranged in a cavity formed between the partition plates; vent holes are reserved between the partition plates and the filter box, and the vent holes of the adjacent partition plates are distributed in a staggered manner; a cover plate is arranged on the filter box; a lock pin is arranged between the cover plate and the filter box; the periphery of the cover plate corresponding to the filter box and the corresponding part of the partition plate are provided with sealing gaskets.

4. The air filtering device for reducing the contamination of amino acid fermentation bacteria according to claim 1, wherein: a pressure gauge and a pressure regulating valve are arranged on the air storage tank; and a one-way valve is arranged on a communicating pipe between the air storage tank and the air compressor.

5. The air filtering device for reducing the contamination of amino acid fermentation bacteria according to claim 1, wherein: two hollow air discs distributed in parallel are arranged in the cooling box; the air discs are connected and communicated through branch pipes; the communicating pipe extends into the cooling box and is respectively connected with the rear sides of the two hollow air discs; and a cooling water circulation interface is arranged on the cooling box.

6. The air filtering device for reducing the contamination of amino acid fermentation bacteria according to claim 1, wherein: and a wire mesh layer is arranged in the dehumidifier.

7. The air filtering device for reducing the contamination of amino acid fermentation bacteria according to claim 1, wherein: the heating box adopts electrical heating, and a heating wire is wound on a pipeline for air circulation.

8. The air filtering device for reducing the contamination of amino acid fermentation bacteria according to claim 1, wherein: the air main filter is divided into two chambers which are symmetrically distributed by a middle partition plate, and two ends of each chamber are respectively connected with the communicating pipe through branch pipes with control valves; a filter cylinder and a corresponding sterilizing tube are arranged in the chamber; the communicating pipe at the air inlet end enters two chambers of the air main filter through the branch pipe, and the branch pipe is connected with the filter cartridge through the flexible joint pipe; a stop block for fixing the filter cartridge is arranged in the chamber.

9. The air filtering device for reducing the contamination of amino acid fermentation bacteria according to claim 1, wherein: the air branch filters are provided with a plurality of groups and are mutually distributed in parallel through branch pipes; and a sub-filter membrane is arranged in the air sub-filter.

10. The air filtering device for reducing the contamination of amino acid fermentation bacteria according to claim 8, wherein: the filter cartridge comprises a membrane body, a bracket and a front cover body; the bracket is used for supporting the membrane body; the front cover body is provided with a threaded hole corresponding to the soft joint pipe; the membrane body is a composite membrane and comprises a plurality of layers of polytetrafluoroethylene membrane layers and PET non-woven fabric layers which are alternately distributed; the membrane pores of the polytetrafluoroethylene membrane layer are 20-50 microns.