CN110898211A - Antibacterial composition for inhibiting pseudosciaena crocea visceral white spot pathogenic bacteria in vitro and preparation method thereof - Google Patents
Antibacterial composition for inhibiting pseudosciaena crocea visceral white spot pathogenic bacteria in vitro and preparation method thereof Download PDFInfo
- Publication number
- CN110898211A CN110898211A CN201911211831.2A CN201911211831A CN110898211A CN 110898211 A CN110898211 A CN 110898211A CN 201911211831 A CN201911211831 A CN 201911211831A CN 110898211 A CN110898211 A CN 110898211A
- Authority
- CN
- China
- Prior art keywords
- nisin
- preparing
- preparation
- composition according
- taking
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/164—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a bacteriostatic composition for inhibiting pseudosciaena crocea visceral white spot pathogenic bacteria in vitro and a preparation method thereof, and relates to the technical field of bacteriostatic compositions, wherein the preparation method of the bacteriostatic composition comprises the following steps: s1. extraction of nisin-B: taking lactobacillus lactis K4 producing nisin-B to perform overnight culture in nutrient broth, taking overnight culture solution of the lactobacillus lactis, adding the nutrient broth into the culture solution, performing constant-temperature culture, performing first centrifugation, taking supernatant, adding saturated ammonium sulfate, standing overnight, performing second centrifugation, taking precipitate, adding sterile distilled water, and preparing a crude extract of nisin-B; s2, preparation of the antibacterial composition: dissolving EDTA-2Na in sterile water, adding the crude extract of nisin-B, and mixing uniformly to obtain the antibacterial composition.
Description
Technical Field
The invention relates to the technical field of antibacterial compositions, and particularly relates to an antibacterial composition for inhibiting pseudosciaena crocea visceral white-spot pathogenic bacteria in vitro and a preparation method thereof.
Background
The large yellow croaker has fresh and tender meat quality and high nutritive value, and is a main offshore economic fish in China. The visceral ichthyophthiriasis of the large yellow croakers is one of the more common diseases of the large yellow croakers cultured in net cages in recent years. Related studies have shown that Pseudomonas spp may be the major pathogen causing visceral ichthyophthiriasis in large yellow croakers. Generally, cage farmers mainly adopt a method of excessively dosing medicines to inhibit pseudomonas during the period of internal ichthyophthiriasis of large yellow croakers. However, the excessive administration of excessive amount of drugs, especially antibiotic drugs, easily causes the deterioration of the culture environment and the generation of pathogenic bacteria resistance. The pseudomonads separated from the diseased large yellow croaker all have drug resistance. In particular to antibiotics such as penicillin, vancomycin, rifampicin, erythromycin, furazolidone, chloramphenicol, compound sulfanilamide, nitrofurantoin and the like. Therefore, screening suitable pseudomonas-inhibiting substances is necessary to solve the problem of visceral white spot disease of large yellow croakers.
The lactobacillus bacteriocin is a polypeptide which is synthesized by lactobacillus ribosome and has the bacteriostatic action on relative bacteria. The only commercially available lactobacillus bacteriocin currently approved by FDA/WHO for addition to food is Nisin (amino acid sequence MSTKDFNLDLVSVSKKDSGASPRITSISLCTPGCKTGALMGCNMKTATCHCSIHVS K), hereinafter referred to as Nisin-a. The inhibition mechanism of Nisin is mainly to anchor the lipid membrane ii (lipid ii) on the cell wall of bacteria, causing the cell structure of bacteria to be loose, thereby killing bacteria. However, due to different structures of bacterial cell walls, Nisin has a remarkable inhibitory effect on gram-positive bacteria only, and has not been found yet. Therefore, Nisin alone does not inhibit Pseudomonas (gram negative bacteria).
A novel Nisin (with an amino acid sequence of MSTKDFNLDLV SVSKKDSGASPRITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK) screened from shellfish in Fujian region is called Nisin-B. Nisi n-B is a variant of Nisin-A, and the two have no difference in bacteriostasis. Nisin-B is extracted from aquatic products, so that the method is more suitable for living environment of aquatic products and provides more choices for bacteriostasis.
At present, no method for inhibiting pseudomonad pathogenic bacteria of pseudosciaena crocea by using a bacteriostatic composition prepared by mixing Nisin-B and EDTA-2Na in the prior art exists.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a preparation method of a bacteriostatic composition for inhibiting pseudosciaena crocea visceral white spot pathogenic bacteria in vitro, wherein the preparation method of the bacteriostatic composition comprises the following steps: s1. extraction of nisin-B: taking lactobacillus lactis K4 producing nisin-B to perform overnight culture in nutrient broth, taking overnight culture solution of the lactobacillus lactis, adding the nutrient broth into the culture solution, performing constant-temperature culture, performing first centrifugation, taking supernatant, adding saturated ammonium sulfate, standing overnight, performing second centrifugation, taking precipitate, adding sterile distilled water, and preparing a crude extract of nisin-B; s2, preparation of the antibacterial composition: dissolving EDTA-2Na in sterile water, adding the crude extract of nisin-B, and mixing uniformly to obtain the antibacterial composition.
The weight part ratio of the lactobacillus lactis K4 to the nutrient broth in S1 is 1-3: 100.
The culture volume of the overnight lactobacillus lactis in S1 was 1% of the original culture volume, the volume of the nutrient broth was 1L, and the overnight culture time was 24 hours.
Conditions for isothermal cultivation described in S1: 30 ℃ and 8 h.
Centrifugation conditions in S1: centrifuging for the first time at 10000rpm for 30 min; second centrifugation, 9500rpm, 30 min.
The volume ratio of the supernatant to the saturated ammonium sulfate in S1 was 100: 45.
The amount of the sterilized distilled water added in S1 was 20 ml.
The temperature of standing overnight in S1 was 4 ℃.
The volume of the crude extract of nisin-B described in S2 can be 20ml, EDTA-2Na can be 0.1g (EDTA-2Na is available from national pharmaceutical products chemical Co., Ltd., CAS number: 6381-92-6), sterile water can be 10ml, and all of the above 3 substances can be prepared at room temperature.
The invention also aims to protect the bacteriostatic composition prepared by the preparation method of the bacteriostatic composition.
The invention has the beneficial effects that: the method utilizes the common EDTA-2Na and nisin-B with lower concentration to prepare the bacteriostatic composition for inhibiting the pseudomonas and has obvious inhibiting effect on the pseudomonas. The antibacterial composition can effectively solve the problem of large yellow croaker visceral white spot disease, and provides a novel antibacterial composition for replacing antibiotics, reducing the drug-resistant bacterial outbreak rate and inhibiting pseudomonas.
Drawings
In order to more clearly illustrate the detailed description of the invention or the technical solutions in the prior art, the drawings that are needed in the detailed description of the invention or the prior art will be briefly described below. Throughout the drawings, like elements or portions are generally identified by like reference numerals. In the drawings, elements or portions are not necessarily drawn to scale.
FIG. 1 is a time-sterilization curve;
in the attached figure, 1-blank control, 2-nisin-B, 3-EDTA-2Na, 4-nisin-B and EDTA-2Na are used as bacteriostatic compositions.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.
It is to be noted that, unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the invention pertains.
Example 1
A preparation method of a bacteriostatic composition for inhibiting pseudosciaena crocea visceral white spot pathogenic bacteria in vitro comprises the following steps: s1. extraction of nisin-B: taking lactobacillus lactis K4 producing nisin-B to perform overnight culture in nutrient broth, taking overnight culture solution of the lactobacillus lactis, adding the nutrient broth into the culture solution, performing constant-temperature culture, performing first centrifugation, taking supernatant, adding saturated ammonium sulfate, standing overnight, performing second centrifugation, taking precipitate, adding sterile distilled water, and preparing a crude extract of nisin-B; s2, preparation of the antibacterial composition: dissolving EDTA-2Na in sterile water, adding the crude extract of nisin-B, and mixing uniformly to obtain the antibacterial composition.
The weight part ratio of the lactobacillus lactis K4 to the nutrient broth in S1 is 1: 100.
The culture volume of the overnight lactobacillus lactis in S1 was 1% of the original culture volume, the volume of the nutrient broth was 1L, and the overnight culture time was 24 hours.
Conditions for isothermal cultivation described in S1: 30 ℃ and 8 h.
Centrifugation conditions in S1: centrifuging for the first time at 10000rpm for 30 min; second centrifugation, 9500rpm, 30 min.
The volume ratio of the supernatant to the saturated ammonium sulfate in S1 was 100: 45.
The amount of the sterilized distilled water added in S1 was 20 ml.
The temperature of standing overnight in S1 was 4 ℃.
Example 2
A preparation method of a bacteriostatic composition for inhibiting pseudosciaena crocea visceral white spot pathogenic bacteria in vitro comprises the following steps: s1. extraction of nisin-B: taking lactobacillus lactis K4 producing nisin-B to perform overnight culture in nutrient broth, taking overnight culture solution of the lactobacillus lactis, adding the nutrient broth into the culture solution, performing constant-temperature culture, performing first centrifugation, taking supernatant, adding saturated ammonium sulfate, standing overnight, performing second centrifugation, taking precipitate, adding sterile distilled water, and preparing a crude extract of nisin-B; s2, preparation of the antibacterial composition: dissolving EDTA-2Na in sterile water, adding the crude extract of nisin-B, and mixing uniformly to obtain the antibacterial composition.
The weight part ratio of the lactobacillus lactis K4 to the nutrient broth in S1 is 2: 100.
The culture volume of the overnight lactobacillus lactis in S1 was 1% of the original culture volume, the volume of the nutrient broth was 1L, and the overnight culture time was 24 hours.
Conditions for isothermal cultivation described in S1: 30 ℃ and 8 h.
Centrifugation conditions in S1: centrifuging for the first time at 10000rpm for 30 min; second centrifugation, 9500rpm, 30 min.
The volume ratio of the supernatant to the saturated ammonium sulfate in S1 was 100: 45.
The amount of the sterilized distilled water added in S1 was 20 ml.
The temperature of standing overnight in S1 was 4 ℃.
Example 3
A preparation method of a bacteriostatic composition for inhibiting pseudosciaena crocea visceral white spot pathogenic bacteria in vitro comprises the following steps: s1. extraction of nisin-B: taking lactobacillus lactis K4 producing nisin-B to perform overnight culture in nutrient broth, taking overnight culture solution of the lactobacillus lactis, adding the nutrient broth into the culture solution, performing constant-temperature culture, performing first centrifugation, taking supernatant, adding saturated ammonium sulfate, standing overnight, performing second centrifugation, taking precipitate, adding sterile distilled water, and preparing a crude extract of nisin-B; s2, preparation of the antibacterial composition: dissolving EDTA-2Na in sterile water, adding the crude extract of nisin-B, and mixing uniformly to obtain the antibacterial composition.
The weight part ratio of the lactobacillus lactis K4 to the nutrient broth in S1 is 3: 100.
The culture volume of the overnight lactobacillus lactis in S1 was 1% of the original culture volume, the volume of the nutrient broth was 1L, and the overnight culture time was 24 hours.
Conditions for isothermal cultivation described in S1: 30 ℃ and 8 h.
Centrifugation conditions in S1: centrifuging for the first time at 10000rpm for 30 min; second centrifugation, 9500rpm, 30 min.
The volume ratio of the supernatant to the saturated ammonium sulfate in S1 was 100: 45.
The amount of the sterilized distilled water added in S1 was 20 ml.
The temperature of standing overnight in S1 was 4 ℃.
Test example 1
Minimum inhibitory concentration detection of pseudomonas
The test method comprises the following steps: 1. inoculating Pseudomonas bacteria to 5mL of Pseudomonas broth, and culturing overnight at 30 deg.C under aerobic condition;
2. pouring 100 mu L of pseudomonas cultured overnight into 5mL of soft agar (0.8% agar powder), fully and uniformly pouring into a brain heart extract culture medium, and after the soft agar is solidified, adopting a sample application method to inhibit pseudomonas by using a bacteriostatic composition prepared by mixing nisin-B with different concentrations, EDTA-2Na with different concentrations and the two components;
3. carrying out constant-temperature culture on the spotted culture medium at the temperature of 30 ℃;
4. observing the culture medium cultured overnight, and recording the concentration of the obvious inhibition zone on the culture medium;
5. mixing nisin-B and EDTA-2Na mixed solution with different concentrations with 100 mu L of overnight cultured pseudomonas by using a 96-well cell plate, culturing the mixture at the temperature of 30 ℃ in a 200 mu L system until the OD600nm of bacteria reaches 0.4-0.5, and recording the minimum inhibition concentration of the mixed solution;
6. the lowest concentration of pseudomonas inhibitory concentration of the mixed solution was added to 5mL of brain-heart leachate, 100 μ L of pseudomonas was added, the mixture was cultured at 30 ℃, and the mixture was spread on a pseudomonas culture medium at regular intervals, and the number of pseudomonas was recorded, and compared with the number of pseudomonas of a blank group (brain-heart leachate to which only pseudomonas was added but no nisin-B + EDTA-2Na was added).
TABLE 1 minimum inhibitory concentration of nisin-B, EDTA-2Na and mixture of both against Pseudomonas
The experimental results show that the minimum inhibitory concentrations of the two against pseudomonas are shown in table 1. Wherein nisin-B still has no inhibition effect on pseudomonas, and the minimum inhibition concentration of EDTA-2Na is 0.4 mg/ml; the nisin-B + EDTA-2Na antibacterial composition has an obvious inhibition effect on pseudomonas, wherein the addition concentration of EDTA-2Na is obviously reduced, the minimum inhibition concentration is reduced to 0.0625mg/ml, and the FIC value of the nisin-B + EDTA-2Na antibacterial composition is calculated to be 0.16 (less than 0.5), namely the nisin-B, EDTA-2Na antibacterial composition prepared by mixing the nisin-B + EDTA-2Na has an obvious synergistic inhibition effect on pseudomonas.
The results of the above experiments prove that the bacteriostatic composition prepared by using common EDTA-2Na and nisin-B with lower concentrations in the method inhibits pseudomonas and has obvious inhibition effect on pseudomonas. The method can effectively solve the problem of large yellow croaker visceral white spot disease, and provides a new method for replacing antibiotics, reducing the outbreak rate of drug-resistant bacteria and inhibiting pseudomonas.
Test example 2
Pseudomonas 24h inhibition number assay
The test method comprises the following steps:
adding 5mL LB liquid culture medium into 4 test tubes (numbered 1, 2, 3 and 4), respectively adding 100 μ L of overnight-cultured Pseudomonas, adding nisin-B into test tube No. 2, wherein the addition of nisin-B is MIC (minimum inhibitory concentration) when the indicator bacterium is inhibited alone; no. 3 test tube is added with EDTA-2Na, and the addition amount of the EDTA-2Na is MIC when the EDTA-2Na alone inhibits the indicator bacterium; test tube No. 4 is the bacteriostatic composition of EDTA-2Na and nisin-B of example 1, and the addition amounts of EDTA-2Na and nisin-B were adjusted to the lowest FIC addition amount in the combination. Initial concentration of bacterial suspension was 2X 107CFU/ml, putting into a constant temperature incubator, culturing at 30 ℃, sampling for 0, 2, 4, 6, 9 and 12 hours, taking 100 mu L of bacterial suspension in 4 test tubes respectively, carrying out gradient dilution in a 1.5ml centrifuge tube, taking 100 mu L of mixed liquor under corresponding dilution, coating, making three parallel groups for each dilution, culturing the coated plate at 30 ℃ for 24 hours, and determining the viable count by adopting a plate counting method. Finally, the time is used as an abscissa, and the logarithm of the total viable count (TVC, unit CFU/mL) is used as an ordinate to plot a time-sterilization curve, as shown in FIG. 1.
As can be seen from FIG. 1, the bacteriostatic composition of example 1 has a significant inhibitory effect on the amount of Pseudomonas bacteria.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.
SEQUENCE LISTING
<110> research institute of aquatic products in east Fujian province
<120> bacteriostatic composition for inhibiting pseudosciaena crocea visceral white spot pathogenic bacteria in vitro and preparation method thereof
<130>
<160>1
<170>PatentIn version 3.3
<210>1
<211>57
<212>PRT
<213>Lactobacillus lactis
<400>1
Met Ser Thr Lys Asp Phe Asn Leu Asp Leu Val Ser Val Ser Lys Lys
1 5 10 15
Asp Ser Gly Ala Ser Pro Arg Ile Thr Ser Ile Ser Leu Cys Thr Pro
20 25 30
Gly Cys Lys Thr Gly Ala Leu Met Gly Cys Asn Met Lys Thr Ala Thr
35 40 45
Cys Asn Cys Ser Ile His Val Ser Lys
50 55
Claims (10)
1. A preparation method of an antibacterial composition for inhibiting pseudosciaena crocea visceral white spot pathogenic bacteria in vitro is characterized by comprising the following steps: the preparation method of the antibacterial composition comprises the following steps: s1. extraction of nisin-B: taking lactobacillus lactis K4 producing nisin-B to perform overnight culture in nutrient broth, taking overnight culture solution of the lactobacillus lactis, adding the nutrient broth into the culture solution, performing constant-temperature culture, performing first centrifugation, taking supernatant, adding saturated ammonium sulfate, standing overnight, performing second centrifugation, taking precipitate, adding sterile distilled water, and preparing a crude extract of nisin-B; s2, preparation of the antibacterial composition: dissolving EDTA-2Na in sterile water, adding the crude extract of nisin-B, and mixing uniformly to obtain the antibacterial composition.
2. A method for preparing a bacteriostatic composition according to claim 1, characterized in that: the weight part ratio of the lactobacillus lactis K4 to the nutrient broth in S1 is 1-3: 100.
3. A method for preparing a bacteriostatic composition according to claim 1, characterized in that: the culture volume of the overnight lactobacillus lactis in S1 was 1% of the original culture volume, the volume of the nutrient broth was 1L, and the overnight culture time was 24 hours.
4. A method for preparing a bacteriostatic composition according to claim 1, characterized in that: conditions for isothermal cultivation described in S1: 30 ℃ and 8 h.
5. A method for preparing a bacteriostatic composition according to claim 1, characterized in that: centrifugation conditions in S1: centrifuging for the first time at 10000rpm for 30 min; second centrifugation, 9500rpm, 30 min.
6. A method for preparing a bacteriostatic composition according to claim 1, characterized in that: the volume ratio of the supernatant to the saturated ammonium sulfate in S1 was 100: 45.
7. A method for preparing a bacteriostatic composition according to claim 1, characterized in that: the amount of the sterilized distilled water added in S1 was 20 ml.
8. A method for preparing a bacteriostatic composition according to claim 1, characterized in that: the temperature of standing overnight in S1 was 4 ℃.
9. A method for preparing a bacteriostatic composition according to claim 1, characterized in that: the volume of the crude extract of nisin-B described in S2 is 20ml, EDTA-2Na0.1g, and 10ml of sterile water.
10. A bacteriostatic composition prepared by the method for preparing the bacteriostatic composition according to any one of claims 1-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911211831.2A CN110898211A (en) | 2019-12-02 | 2019-12-02 | Antibacterial composition for inhibiting pseudosciaena crocea visceral white spot pathogenic bacteria in vitro and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911211831.2A CN110898211A (en) | 2019-12-02 | 2019-12-02 | Antibacterial composition for inhibiting pseudosciaena crocea visceral white spot pathogenic bacteria in vitro and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110898211A true CN110898211A (en) | 2020-03-24 |
Family
ID=69821076
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911211831.2A Pending CN110898211A (en) | 2019-12-02 | 2019-12-02 | Antibacterial composition for inhibiting pseudosciaena crocea visceral white spot pathogenic bacteria in vitro and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110898211A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993013793A1 (en) * | 1992-01-17 | 1993-07-22 | Applied Microbiology, Inc. | Pharmaceutical bacteriocin compositions |
| CN101066994A (en) * | 2007-04-20 | 2007-11-07 | 上海奇泓生物科技有限公司 | Process of extracting lactic streptocin from fermented liquid |
| CN101558781A (en) * | 2009-05-14 | 2009-10-21 | 浙江工商大学 | Composite biological antistaling agent for minced fillet product and method for using same |
| CN102250806A (en) * | 2011-07-01 | 2011-11-23 | 安徽农业大学 | Method for screening lactic acid bacteria capable of producing bacteriocin from plant source materials |
| CN104774890A (en) * | 2015-04-13 | 2015-07-15 | 光明乳业股份有限公司 | Paenibacillus sp. bacteriocin crude extract as well as preparation method and application thereof |
-
2019
- 2019-12-02 CN CN201911211831.2A patent/CN110898211A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993013793A1 (en) * | 1992-01-17 | 1993-07-22 | Applied Microbiology, Inc. | Pharmaceutical bacteriocin compositions |
| CN101066994A (en) * | 2007-04-20 | 2007-11-07 | 上海奇泓生物科技有限公司 | Process of extracting lactic streptocin from fermented liquid |
| CN101558781A (en) * | 2009-05-14 | 2009-10-21 | 浙江工商大学 | Composite biological antistaling agent for minced fillet product and method for using same |
| CN102250806A (en) * | 2011-07-01 | 2011-11-23 | 安徽农业大学 | Method for screening lactic acid bacteria capable of producing bacteriocin from plant source materials |
| CN104774890A (en) * | 2015-04-13 | 2015-07-15 | 光明乳业股份有限公司 | Paenibacillus sp. bacteriocin crude extract as well as preparation method and application thereof |
Non-Patent Citations (5)
| Title |
|---|
| 唐春红: "《天然防腐剂与抗氧化剂》", 31 May 2010, 中国轻工业出版社 * |
| 江汉湖: "《食品微生物学》", 31 August 2002, 中国农业出版社 * |
| 王国良等: "《大黄鱼主要病害临床诊断和防治手册》", 30 April 2013, 厦门大学出版社 * |
| 邓舜扬: "《食品保鲜技术》", 31 January 2006, 中国轻工业出版社 * |
| 郝素娥等: "《食品添加剂制备与应用技术》", 31 March 2003, 化学工业出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101536901B1 (en) | Probiotics composition for fishes containing a mixture of Bacillus subtilis and phage | |
| Wang et al. | Current developments of bacteriocins, screening methods and their application in aquaculture and aquatic products | |
| CN110819548A (en) | Lactobacillus paracasei strain LBP-YE01, culture thereof, bacterial solution thereof, method for selecting and preserving subculture strain and application thereof | |
| CN114085789A (en) | Pediococcus pentosaceus MA.WTPQJ01 and application thereof | |
| WO2020031191A1 (en) | Microorganism mixtures, molecules derived therefrom, and methods of use thereof | |
| CN113040390A (en) | Probiotic and salt-tolerant Lactobacillus johnsonii strain and application thereof in prevention and treatment of pathogenic bacteria in livestock and poultry aquaculture | |
| KR101471033B1 (en) | Novel Strains of Weissella sp. F22 with Anti-Obesity Ability | |
| TWI433651B (en) | Novel pediococcus pentosaceus and its composition and use thereof | |
| CN109652334A (en) | A kind of complex microbial inoculum and its preparation method and application | |
| CN110898211A (en) | Antibacterial composition for inhibiting pseudosciaena crocea visceral white spot pathogenic bacteria in vitro and preparation method thereof | |
| CN117448213B (en) | Lactobacillus plantarum for inhibiting clostridium perfringens and its progeny and application | |
| CN109985027B (en) | Application of myricetin in preparation of gram-negative bacterium virulence resisting medicine | |
| CN116064324B (en) | Lactobacillus rhamnosus, culture method thereof and application thereof in preventing and treating diarrhea and enteritis | |
| CN114717150B (en) | Lactobacillus plantarum CRS33 and application thereof | |
| CN117126778A (en) | Bacillus bailii and application thereof | |
| CN116875502A (en) | Composite microbial preparation and application thereof | |
| CN114869871A (en) | Application of pterostilbene in resisting infection of aquatic aeromonas hydrophila of aquaculture animals | |
| RU2393214C1 (en) | Immunobiologial antiallergic agent (versions), lactobacillus acidophilus nkjc strain, lactobacillus acidophilus jch strain, lactobacillus acidophilus kaa strain | |
| NL2032407B1 (en) | Screening and Application of Enterococcus faecium G12 | |
| CN115975846B (en) | Streptomyces saramycin, microecological preparation and preparation method thereof | |
| RU2735230C1 (en) | Method for increasing cicatric digestion in ruminant cattle animals | |
| CN113368137B (en) | Natural bacterial quorum sensing inhibitor and application thereof | |
| CN118222516B (en) | Phage RDP-SA-21030 capable of lysing salmonella at stable phase, bactericidal composition and application thereof | |
| CN106749538B (en) | Polypeptide capable of blocking drug-resistant bacteria quorum sensing system and application thereof | |
| TW201136529A (en) | A lactobacillus and its use in aquaculture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200324 |
|
| RJ01 | Rejection of invention patent application after publication |