CN110743634B - Micro-fluidic device - Google Patents
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- CN110743634B CN110743634B CN201910890656.8A CN201910890656A CN110743634B CN 110743634 B CN110743634 B CN 110743634B CN 201910890656 A CN201910890656 A CN 201910890656A CN 110743634 B CN110743634 B CN 110743634B
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- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The present invention relates to a microfluidic device comprising: a microfluidic chip for generating droplets; the oil phase injection device comprises an oil phase injector for injecting oil phase into the microfluidic chip, wherein the top end of the oil phase injector is provided with an oil phase injection port communicated with the microfluidic chip, and an oil phase piston moving towards the oil phase injection port along the inner wall of the oil phase injector is arranged in the oil phase injector; the water phase injection device comprises a water phase injector for injecting water phase into the micro-fluidic chip, a water phase injection port communicated with the micro-fluidic chip is arranged at the top end of the water phase injector, and a water phase piston moving towards the water phase injection port along the inner wall of the water phase injector is arranged in the water phase injector. The invention adopts the injector to inject liquid, and separates the droplets wrapping the air from the required microemulsion droplets, thereby improving the product quality.
Description
Technical Field
The invention relates to the technical field of microfluidic control, in particular to microfluidic equipment.
Background
Microfluidics (Microfluidics) refers to the science and technology involved in systems that use microchannels (tens to hundreds of microns in size) to process or manipulate tiny fluids (nanoliters to attoliters in volume). The technology is currently more researched on precise medical treatment and biological materials, can also be used for preparing monodisperse emulsion, and particularly has good technical advantages on preparing multiple emulsions or microcapsules.
Chinese patent No. CN201721802854.7 discloses a microfluidic preparation device of polymer microemulsion, referring to fig. 1 and fig. 2, fig. 1 is a schematic structural diagram of the microfluidic preparation device of polymer microemulsion, and fig. 2 is an enlarged schematic diagram of a point a in fig. 1. The microfluidic preparation device comprises a controller 1, a stage 2, a first injection device 3, a second injection device 4 and a droplet generation chip 5. The droplet generation chip 5 is provided on the stage 2, and the droplet generation chip 5 is provided with a first inlet 51, a first inlet 52, a first channel 53, and a second channel 54. One end of the first channel 53 is connected to the first injection port 51, and the other end of the first channel 53 is an emulsion outlet 55. The second channel 54 includes a first branch 541 and a second branch 542, one ends of the first branch 541 and the second branch 542 are both communicated with the first injection port 52, and the other ends of the first branch 541 and the second branch 542 are intersected with the first channel 53 at a point. The first injection device 3 comprises a first injector 31 and a first injection pump 32, wherein the discharge end of the first injector 31 is communicated with the first injection port 51 through a pipeline, and the first injection pump 32 is electrically connected with the controller 1. The second injection device 4 comprises a second injector 41 and a second injection pump 42, wherein the discharge end of the second injector 41 is communicated with the first injection port 52 through a pipeline, and the second injection pump 42 is electrically connected with the controller 1.
The working principle of the microfluidic preparation device is as follows: loading an oil phase (e.g., an oil phase of 0.05g of polystyrene particles dissolved in 5mL of chloroform) into the first syringe 31, and placing the first syringe 31 loaded with the oil phase on the first syringe pump 32; the aqueous phase (e.g., an aqueous phase of 0.1g 1% tween 80 and 0.1g 1% glycerol in 10mL of water) is loaded into the second syringe 41, and the second syringe 41 with the aqueous phase is placed on the second syringe pump 42; according to the size requirement of the required microemulsion droplets, the controller 1 controls the first injection pump 32 and the second injection pump 42 to respectively inject an oil phase and a water phase into the first injection port 51 and the first injection port 52 on the droplet generation chip 5, and the flow rates of the water phase and the oil phase are controlled to be different (for example, the flow rate ratio is that the water phase: the oil phase is 45 muL/min: 1045 muL/min); the oil phase enters the first channel 53 through the first injection port 51, the water phase enters the first branch 541 and the second branch 542 through the first injection port 52, and then meets the oil phase at the intersection point of the first branch 541, the second branch 542 and the first channel 53, and due to the flow speed difference of the water phase and the oil phase, the linear oil phase is extruded and broken, so that the dispersed microemulsion droplets are obtained.
According to the micro-fluidic preparation device, the linear oil phase is extruded to form dispersed liquid drops through the flow speed difference between the water phase and the oil phase, so that the liquid drops with good uniformity are obtained. However, the injector of the microfluidic preparation device is horizontally arranged, the injection port of the injector is positioned at one end of the injector, and the piston in the injector horizontally moves along the inner wall of the injector. When the syringe is used for fluid infusion, the liquid can carry a trace amount of air into the syringe pump, so that the syringe is filled with the trace amount of air. During the process of injecting liquid into the droplet generation chip 5 by the injector, the injector injects the trace amount of air into the microfluidic chip, which may cause the continuous droplets to be doped with unqualified droplets during the formation process of the continuous droplets, thereby greatly reducing the product quality.
Disclosure of Invention
Based on this, the present invention aims to provide a microfluidic device which also uses an injector to inject liquid, but which can separate air-encapsulated microemulsion droplets from desired microemulsion droplets, thereby contributing to the improvement of product quality.
A microfluidic device comprising:
a microfluidic chip for generating droplets;
the oil phase injection device comprises an oil phase injector for injecting oil phase into the microfluidic chip, the top end of the oil phase injector is provided with an oil phase injection port communicated with the microfluidic chip, and an oil phase piston moving towards the oil phase injection port along the inner wall of the oil phase injector is arranged in the oil phase injector;
the water phase injection device comprises a water phase injector for injecting water phase into the microfluidic chip, a water phase injection port communicated with the microfluidic chip is formed in the top end of the water phase injector, and a water phase piston moving towards the water phase injection port along the inner wall of the water phase injector is arranged in the water phase injector.
Compared with the prior art, when the injector replenishes the liquid phase, the piston moves downwards, the injector injects the liquid phase, and during the process, air in the injector flows to the top of the cavity of the injector; after the injector is supplemented, the cavity of the injector is divided into an air layer and a liquid layer which are an upper layer and a lower layer; when the injector injects the liquid phase, the piston moves upwards along the inner wall of the injector, air is firstly discharged from the injector, and when the air in the injector is completely discharged, the liquid phase is discharged from the injector. Through the injection mode, the liquid drops carrying air can be effectively prevented from being mixed in the continuous liquid drop forming process, and the product quality is improved.
The liquid distribution device is used for controlling the flow direction of liquid drops and comprises a two-position three-way electromagnetic valve, wherein a liquid inlet communicated with an outlet of the microfluidic chip, a first discharge hole for qualified liquid drops to flow out and a second discharge hole for unqualified liquid drops to flow out are formed in the two-position three-way electromagnetic valve. By adopting the scheme, the liquid drops carrying air are uniformly sent into the container by the liquid separating device, and the qualified liquid drops are uniformly sent into the other container, so that the improvement of the product quality is facilitated. And, simple structure can control the flow direction of liquid drop fast and accurately.
Furthermore, an output port for discharging qualified liquid drops on the two-position three-way electromagnetic valve is connected with a liquid drop electric pump. By adopting the scheme, the flow velocity of qualified liquid drops can be accelerated, and the production efficiency is improved.
The industrial personal computer is electrically connected with the oil phase injection device, the water phase injection device and the microscope camera respectively, integrates a video identification technology, can judge the size of the generated liquid drop in real time and gives feedback information to control the flow speed of the oil phase or the water phase. By adopting the scheme, the industrial personal computer identifies and analyzes the image of the picture shot by the microscope camera, and controls the flow direction of the unqualified liquid drops and the qualified liquid drops through the liquid separating device according to the analysis result, so that the unqualified liquid drops and the qualified liquid drops are completely separated, and the automatic production is realized.
Further, a first edible pigment is added into the oil phase, a second edible pigment is added into the water phase, the color of the first edible pigment is different from that of the second edible pigment, the color of the first edible pigment is green, and the color of the second edible pigment is red. By adopting the scheme, the color of the oil phase is inconsistent with the color of the water phase, and the image recognition efficiency and accuracy are accelerated. The color of the first edible pigment is green, and the color of the second edible pigment is red, so that the recognition rate between the oil phase color and the water phase color is the highest, and the image recognition efficiency and accuracy are further accelerated.
Further, the device also comprises a transparent chip with a transparent pipeline, wherein the transparent chip is positioned in the shooting range of the microscope camera, and one end of the transparent pipeline is connected with the second discharge hole. By adopting the scheme, when the industrial personal computer identifies that the micro-fluidic chip generates qualified liquid drops and outputs the liquid drops meeting the requirements in the transparent pipeline, the liquid dividing device changes the flow direction of the subsequent liquid drops, so that the qualified liquid drops are further prevented from being doped with unqualified liquid drops, and the product quality is improved.
Further, an ultrasonic module is also included for eliminating air in the oil phase injector or for discharging air in the water phase injector. By adopting the scheme, air in the oil phase or the water phase is discharged through ultrasonic waves, so that the air flows to the top of the oil phase injector or the water phase injector, and the product quality is further improved.
Further, the oil phase injector is vertically arranged, and the oil phase piston vertically moves up and down along the inner wall of the oil phase injector; the water phase injector is vertically arranged, and the water phase piston vertically moves up and down along the inner wall of the water phase injector; also comprises an oil phase container and a water phase container; the oil phase injection device also comprises an oil phase throttling valve, an oil phase first one-way valve, an oil phase reversing valve, an oil phase electric pump, an oil phase second one-way valve, an oil phase third one-way valve and an oil phase overflow valve, an oil phase injection port of the oil phase injector is communicated to the microfluidic chip through an oil phase throttling valve and an oil phase first one-way valve in sequence, the oil phase reversing valve is provided with a main oil port, a first working oil port, a second working oil port and an oil return port, the oil phase container is connected to the oil phase injector through an oil phase valve, an oil phase electric pump, a main oil port, a first working oil port and an oil phase second one-way valve in sequence to push the piston to move, the oil phase injector is connected to the oil phase container through a second working oil port, an oil return port and an oil phase third one-way valve in sequence to recover the oil phase, an oil phase overflow valve is arranged between the outlet of the oil phase electric pump and the outlet of the oil phase third one-way valve; the water phase injection device also comprises a water phase throttling valve, a water phase first one-way valve, a water phase reversing valve, a water phase electric pump, a water phase second one-way valve, a water phase third one-way valve and a water phase overflow valve, a water phase injection port of the water phase injector is communicated to the micro-fluidic chip through a water phase throttle valve and a water phase first one-way valve in sequence, the water phase reversing valve is provided with a main water port, a first working water port, a second working water port and a water return port, the water phase container is connected to the water phase injector through a water phase valve, a water phase electric pump, a main water port, a first working water port and a water phase second one-way valve in sequence to push the piston to move, the water phase injector is connected to the water phase container through a second working water gap, a water return port and a water phase third one-way valve in sequence to recover the water phase, and a water phase overflow valve is arranged between the outlet of the water phase electric pump and the outlet of the water phase third one-way valve. By adopting the scheme, the installation state of the injector is set, so that the liquid phase is injected after the air is exhausted by the injector, and the quality of liquid drops is further improved. And the oil phase injection device and the water phase injection device have simple structures, and can realize automatic injection of the oil phase and the water phase and supplement of the oil phase and the water phase.
Further, the micro-fluidic chip is generated by micro-nano 3D printing and made of a transparent material, a liquid outlet pipeline and at least 5 liquid drop generating pipelines communicated with the liquid outlet pipeline are arranged in the micro-fluidic chip, each liquid drop generating pipeline comprises an outer pipeline and an inner pipeline, a liquid outlet end of each inner pipeline is arranged in the outer pipeline, a liquid outlet direction of each inner pipeline is parallel to a liquid flowing direction in the outer pipeline, an outer pipe wall of the liquid outlet end of each inner pipeline is conical, a liquid outlet end of each outer pipeline is connected with the liquid outlet pipeline, each outer pipeline is used for feeding any one of an oil phase and a water phase, and correspondingly, each inner pipeline is used for feeding the other of the oil phase and the water phase. By adopting the scheme, the micro-fluidic chip is manufactured by micro-nano 3D printing, so that the design diversity of the channel structure of the micro-fluidic chip is improved conveniently, and the channel structure can be designed into a three-dimensional structure. In addition, with the above channel structure, the microfluidic chip can generate oil-in-water droplets or water-in-oil droplets. And only the aperture of the inner pipeline needs to be designed into a smaller aperture, and the aperture of the outer pipeline does not need to be designed into a smaller aperture, namely, the aperture of the inner pipeline only has the processing precision requirement, and the aperture of the outer pipeline can be properly increased, so that the processing difficulty of the micro-fluidic chip is favorably reduced, and the liquid drop meeting the requirement is favorably obtained. And, because the aperture of outer pipeline is great, help reducing the flow resistance of liquid.
Further, the liquid drop generating pipeline further comprises a secondary pipeline, the liquid outlet end of the outer pipeline is arranged in the secondary pipeline, the liquid outlet direction of the outer pipeline is parallel to the liquid flowing direction in the secondary pipeline, the outer pipe wall of the liquid outlet end of the outer pipeline is conical, the liquid outlet end of the secondary pipeline is connected with the liquid outlet pipeline, and the liquid phase sent by the secondary pipeline is the same as the liquid phase sent by the inner pipeline. By adopting the scheme, the micro-fluidic chip can generate the liquid drop of water-in-oil-in-water type or the liquid drop of water-in-oil type.
For a better understanding and practice, the invention is described in detail below with reference to the accompanying drawings.
Drawings
FIG. 1 is a schematic structural diagram of a microfluidic preparation device for polymer microemulsion;
FIG. 2 is an enlarged schematic view at A in FIG. 1;
fig. 3 is a schematic structural diagram of a microfluidic device according to the first embodiment;
fig. 4 is a schematic piping diagram of a microfluidic device according to the first embodiment;
FIG. 5 is a schematic structural diagram of a microfluidic chip according to the first embodiment;
FIG. 6 is a schematic structural diagram of an oil phase injector and a water phase injector according to the first embodiment;
FIG. 7 is a schematic diagram of an electrical communication of the microfluidic chip according to the first embodiment;
FIG. 8 is a schematic piping diagram of a microfluidic device according to the second embodiment;
FIG. 9 is a schematic structural view of a droplet generation circuit according to the third embodiment;
fig. 10 is a first schematic piping diagram of the microfluidic device according to the third embodiment;
fig. 11 is a second schematic piping diagram of the microfluidic device according to the third embodiment;
description of the drawings:
1. a controller; 2. an object stage; 3. a first injection device; 31. a first syringe; 32. a first syringe pump; 4. a second injection device; 41. a second syringe; 42. a second syringe pump; 5. a droplet generation chip; 51. a first injection port; 52. a first injection port; 53. a first channel; 54. a second channel; 541. a first branch; 542. a second branch circuit; 55. an emulsion outlet;
100. a machine platform; 110. a display screen; 200. a microfluidic chip; 210. an oil phase main channel; 220. a main aqueous phase channel; 230. a droplet generation conduit; 231. an outer conduit; 232. an inner conduit; 233. a secondary pipeline; 240. a liquid outlet pipeline; 300. an oil phase container; 400. an aqueous phase container; 500. an oil phase injection device; 510. an oil phase injector; 511. an oil phase injection port; 512. an oil phase piston; 520. an oil phase throttling valve; 530. an oil phase first one-way valve; 540. an oil phase reversing valve; 541. a first working oil port; 542. a second working oil port; 543. a main oil port; 544. an oil return port; 550. an oil phase second one-way valve; 560. an oil phase valve; 570. an oil phase electric pump; 580. an oil phase third one-way valve; 590. an oil phase overflow valve; 600. an aqueous phase injection device; 610. a water phase injector; 611. a water phase injection port; 612. a water phase piston; 620. a water phase throttle valve; 630. a water phase first one-way valve; 640. a water phase reversing valve; 641. a first working gate; 642. a second working water gap; 643. a main water gap; 644. a water return port; 650. a water phase second one-way valve; 660. a water phase valve; 670. a water phase electric pump; 680. a water phase third one-way valve; 690. a water phase overflow valve; 700. a microscope camera; 800. a height adjustment device; 810. a linear motor; 820. a movable seat; 900. a liquid separating device; 910. a two-position three-way electromagnetic valve; 920. a droplet motor pump; 1000. a waste liquid container; 1100. a collection container; 1200. an industrial personal computer; 1300. an ultrasonic module; 1400. a transparent chip; 1410 a transparent tube.
Detailed Description
Example one
A microfluidic device, see fig. 3 and 4, includes a machine 100, a microfluidic chip 200, an oil phase container 300, a water phase container 400, an oil phase injection device 500, a water phase injection device 600, a microscope camera 700, a height adjustment device 800, a liquid separation device 900, a waste liquid container 1000, a collection container 1100, and an industrial personal computer 1200. Wherein, the microfluidic chip 200 is placed on the machine 100. The oil phase injection device 500 injects the oil phase in the oil phase container 300 into the microfluidic chip 200. The aqueous phase injection device 600 injects the aqueous phase in the aqueous phase container 400 into the microfluidic chip 200. The liquid separating device 900 is connected with an outlet of the microfluidic chip 200, and is used for controlling the flow direction of liquid drops, a first discharge port for qualified liquid drops to flow out and a second discharge port for unqualified liquid drops to flow out are arranged on the liquid separating device, the first discharge port is connected to the collecting container 1100, and the second discharge port is connected to the waste liquid container 1000. The microscope camera 700 is used to photograph the droplet generation process in the microfluidic chip 200 in real time. The height adjustment device 800 is used to adjust the vertical distance from the microscope camera 700 to the microfluidic chip 200. The industrial personal computer 1200 is electrically connected with the oil phase injection device 500, the water phase injection device 600, the microscope camera 700, the height adjusting device 800 and the liquid separating device 900 respectively. The industrial personal computer 1200 obtains dispersed droplets by controlling a difference in flow rate between the oil phase injection device 500 and the water phase injection device 600. Meanwhile, the industrial personal computer 1200 integrates a video recognition technology, which is pointed out here to belong to the prior art, and as long as the industrial personal computer 1200 can perform image recognition, it is not discussed here. Specifically, the industrial personal computer 1200 identifies an image acquired by the microscope camera 700, and determines the condition of generating liquid droplets in real time, and if the liquid droplets are waste liquid, the industrial personal computer 1200 discharges the liquid droplets into the waste liquid container 1000 through the liquid separation device 900; if the droplets are the desired emulsion, industrial computer 1200 drains the droplets through a liquid separation device 900 into a collection vessel 1100.
Referring to fig. 4 and 5, the microfluidic chip 200 is produced by micro-nano 3D printing. As shown in fig. 5, the microfluidic chip 200 is made of transparent material, and five independent droplet generation units are integrated, and in practical application, the number of arrays can be increased according to requirements. An oil phase main channel 210, a water phase main channel 220, five droplet generation pipelines 230 and a liquid outlet pipeline 240 are arranged in the micro-fluidic chip 200, wherein the oil phase main channel 210 injects oil phase into all the droplet generation pipelines, the water phase main channel 220 injects water phase into all the droplet generation pipelines, all the droplet generation pipelines deliver the generated droplets into the liquid outlet pipeline 240, and all the droplets are delivered to the outside from the liquid outlet pipeline 240 in a unified manner. Specifically, the droplet generation line 230 includes an outer line 231 and an inner line 232. The inlet of the outer pipe 231 is communicated with the main water phase pipe, and the outlet of the outer pipe 231 is communicated with the liquid outlet pipe 240. The inlet of the inner pipe 232 is communicated with the main oil phase pipe, and the outlet of the inner pipe 232 is connected into the outer pipe 231. The outer pipe wall of the liquid outlet end of the inner pipe 232 is tapered, the liquid outlet end of the inner pipe 232 is located in the outer pipe 231, the outer diameter of the inner pipe 232 is smaller than the inner diameter of the outer pipe 231, the liquid outlet direction of the inner pipe 232 is parallel to the liquid phase flow direction in the outer pipe 231, and the inner pipe 232 is communicated with the liquid outlet pipe 240 through the outer pipe 231. Further, in order to improve the droplet generation efficiency and the droplet quality, the center line of the portion of the inner pipe 232 located on the outer pipe 231 is located on the same line as the center line of the outer pipe 231.
The micro-fluidic chip 200 is manufactured by micro-nano 3D printing, so that the design diversity of the channel structure of the micro-fluidic chip 200 is improved conveniently, and the channel structure can be designed into a three-dimensional structure. Further, the microfluidic chip 200 adopts the above channel structure, only the aperture of the inner pipe 232 needs to be designed to be a smaller aperture, and the aperture of the outer pipe 231 does not need to be designed to be a smaller aperture, that is, only the aperture of the inner pipe 232 has a requirement on the processing precision, and the aperture of the outer pipe 231 can be properly increased, which is beneficial to reducing the processing difficulty of the microfluidic chip 200 and obtaining a droplet meeting the requirement. Also, since the aperture of the outer pipe 231 is large, it helps to reduce the flow resistance of the liquid.
Referring to fig. 3, 4, and 6, the oil phase container 300 is a metal casing for storing oil phase. Further, in order to facilitate the extraction of the oil phase from the oil phase container 300, the bottom of the oil phase container 300 is designed to be funnel-shaped, so that the oil phase can converge toward the outlet at the bottom end of the oil phase container 300.
Referring to fig. 3, 4, 6, the aqueous phase container 400 is a metal housing for storing the aqueous phase. Further, in order to facilitate the extraction of the aqueous phase from the aqueous phase container 400, the bottom of the aqueous phase container 400 is designed to be funnel-shaped, so that the aqueous phase can be converged toward the outlet at the bottom end of the aqueous phase container 400.
In addition, in order to facilitate image recognition, the oil phase is added with a first edible pigment, the water phase is added with a second edible pigment, in order to improve the product quality and ensure that the waste liquid is completely separated from the required liquid drops, and the color of the edible pigment in the water phase is different from that of the edible pigment in the oil phase.
Referring to fig. 3, 4, and 6, oil phase injection device 500 includes oil phase injector 510, oil phase throttle valve 520, oil phase first check valve 530, oil phase reversing valve 540, oil phase second check valve 550, oil phase valve 560, oil phase electric pump 570, oil phase third check valve 580, and oil phase overflow valve 590. The oil phase injector 510 is vertically arranged, an oil phase piston 512 is sleeved in an inner cavity of the oil phase injector 510, the oil phase piston 512 divides the inner cavity of the oil phase injector 510 into an upper cavity and a lower cavity, and the oil phase piston 512 performs reciprocating motion from the top of the oil phase injector 510 to the bottom of the oil phase injector 510 in the inner cavity of the oil phase injector 510. An oil phase injection port 511 is arranged at the top end of the oil phase injector 510, the oil phase injection port 511 is communicated with the upper cavity of the oil phase cylinder body, and the oil phase injection port 511 is communicated with the oil phase main channel 210 through an oil phase throttling valve 520 and an oil phase first one-way valve 530 in sequence. The oil phase change valve 540 is provided with a first working port 541, a second working port 542, a main oil port 543, and an oil return port 544. The first working oil port 541 of the oil phase change valve 540 is connected to the upper chamber of the oil phase injector 510 through the oil phase second change valve. The second working oil port 542 of the oil phase change valve 540 is connected to the lower chamber of the oil phase injector 510. Oil phase container 300 is connected to main oil port 543 of oil phase change valve 540 sequentially through oil phase valve 560 and oil phase electric pump 570, oil phase container 300 is further connected to oil return port 544 of oil phase change valve 540 through oil phase third check valve 580, and oil phase relief valve 590 is installed between the outlet of oil phase electric pump 570 and the outlet of oil phase third check valve 580. In this embodiment, oil phase valve 560 is an existing valve, oil phase electric pump 570 is a centrifugal pump, oil phase reversing valve 540 is an existing two-way four-position electromagnetic valve, oil phase first check valve 530, oil phase second check valve 550, and oil phase third check valve 580 are all existing check valves, oil phase throttling valve 520 is an existing adjustable throttling valve, and oil phase overflow valve 590 is an existing overflow valve.
Referring to fig. 3, 4, and 6, the aqueous phase injection device 600 includes an aqueous phase injector 610, an aqueous phase throttle valve 620, an aqueous phase first check valve 630, an aqueous phase reversing valve 640, an aqueous phase second check valve 650, an aqueous phase valve 660, an aqueous phase electric pump 670, an aqueous phase third check valve 680, and an aqueous phase overflow valve 690. The water phase injector 610 is vertically arranged, a water phase piston 612 is sleeved in an inner cavity of the water phase injector 610, the inner cavity of the water phase injector 610 is divided into an upper cavity and a lower cavity by the water phase piston 612, and the water phase piston 612 does reciprocating motion from the top of the water phase injector 610 to the bottom of the water phase injector 610 in the inner cavity of the water phase injector 610. The top end of the water phase injector 610 is provided with a water phase injection port 611, the water phase injection port 611 is communicated with the upper cavity of the water phase cylinder, and the water phase injection port 611 is communicated with the water phase main channel 220 through a water phase throttling valve 620 and a water phase first one-way valve 630 in sequence. The water phase reversing valve 640 is provided with a first working water gap 641, a second working water gap 642, a main water gap 643 and a water return port 644. The first working water port 641 of the water phase reversing valve 640 is connected with the upper cavity of the water phase injector 610 through the water phase second reversing valve. The second working water port 642 of the aqueous phase reversing valve 640 is connected to the lower chamber of the aqueous phase injector 610. The water phase container 400 is connected with a main water port 643 of the water phase reversing valve 640 through a water phase valve 660 and a water phase electric pump 670 in sequence, the water phase container 400 is further connected with a water return port 644 of the water phase reversing valve 640 through a water phase third check valve 680, and a water phase overflow valve 690 is arranged between the outlet of the water phase electric pump 670 and the outlet of the water phase third check valve 680. In this embodiment, the water phase valve 660 is an existing valve, the water phase electric pump 670 is a centrifugal pump, the water phase reversing valve 640 is an existing two-way four-position electromagnetic valve, the water phase first check valve 630, the water phase second check valve 650, and the water phase third check valve 680 are all existing check valves, the water phase throttle valve 620 is an existing adjustable throttle valve, and the water phase overflow valve 690 is an existing overflow valve.
In addition, in order to further discharge air to the top of the inner cavity of the syringe, the ultrasonic module 1300 is disposed on the outer sides of the oil phase syringe 510 and the water phase syringe 610, and the air of the oil phase or the water phase is vibrated by the ultrasonic module 1300.
Referring to fig. 4, the liquid separating device 900 includes a two-position three-way electromagnetic valve 910, and the liquid outlet pipe 240 of the microfluidic chip 200 is respectively communicated with the waste liquid container 1000 and the collecting container 1100 through the two-position three-way electromagnetic valve 910. In order to improve efficiency, a droplet electric pump 920 is further disposed between the two-position three-way solenoid valve 910 and the collection container 1100, and in this embodiment, the droplet electric pump 920 is a centrifugal pump.
Referring to fig. 3 and 6, the height adjusting device 800 is a linear motor 810 in the prior art, the linear motor 810 is mounted on the machine 100 through bolts, a moving base 820 is disposed on the linear motor 810, and the moving base 820 moves up and down on the linear motor 810 in the vertical direction.
Referring to fig. 3 and 6, the microscope camera 700 is detachably mounted on the movable base 820, the micro-fluidic chip 200 is contained in the photographing area of the microscope camera 700, and the microscope camera 700 is used for observing the micro-fluidic chip 200.
Referring to fig. 7, the industrial personal computer 1200 is electrically connected to the oil phase electric pump 570, the oil phase reversing valve 540, the water phase electric pump 670, the water phase reversing valve 640, the ultrasonic module 1300, the two-position three-way solenoid valve 910, the droplet electric pump 920, the linear motor 810, and the microscope camera 700, respectively. The industrial personal computer 1200 is provided with a display screen 110, and a worker can control the operation of the machine and observe the condition of the microfluidic chip 200 through the display screen 110.
The liquid supplementing process of the injector comprises the following steps: opening the oil phase valve 560 and the water phase valve 660, and starting the oil phase electric pump 570 and the water phase electric pump 670 by the industrial personal computer 1200; wherein, the oil phase in the oil phase container 300 sequentially passes through the oil phase valve 560, the oil phase electric pump 570, the main oil port 543 of the oil phase reversing valve 540, the first working oil port 541 of the oil phase reversing valve 540 and the oil phase second one-way valve 550 to enter the upper cavity of the oil phase injector 510, and the oil phase in the lower cavity of the oil phase injector 510 sequentially passes through the second working oil port 542 of the oil phase reversing valve 540, the oil return port 544 of the oil phase reversing valve 540 and the oil phase third reversing valve to flow into the oil phase container 300; the water phase in the water phase container 400 sequentially passes through the water phase valve 660, the water phase electric pump 670, the main water port 643 of the water phase reversing valve 640, the first working water port 641 of the water phase reversing valve 640 and the water phase second one-way valve 650 to enter the upper cavity of the water phase injector 610, and the water phase in the lower cavity of the water phase injector 610 sequentially passes through the second working water port 642 of the water phase reversing valve 640, the water return port 644 of the water phase reversing valve 640 and the water phase third reversing valve to flow into the water phase container 400; meanwhile, the industrial personal computer 1200 starts the ultrasonic module 1300 to work, air in the oil phase injector 510 flows to the top of the upper cavity of the oil phase injector 510, and air in the water phase injector 610 flows to the top of the upper cavity of the water phase injector 610; finally, an air layer and an oil phase layer are formed in the upper cavity of the oil phase injector 510, and the air layer is positioned above the oil phase layer; an air layer and an aqueous phase layer are formed in the upper chamber of the aqueous phase injector 610, and the air layer is located above the aqueous phase layer.
The injection process of the injector comprises the following steps: after the liquid is supplemented, the industrial personal computer 1200 respectively controls the oil phase reversing valve 540 and the water phase reversing valve 640 to reverse, the first working oil port 541 is connected to the lower cavity of the oil phase injector 510, the second working oil port 542 is connected to the upper cavity of the oil phase injector 510, the first working water port 641 is connected to the lower cavity of the water phase injector 610, and the second working water port 642 is connected to the upper cavity of the water phase injector 610; then, the oil phase in the oil phase container 300 sequentially passes through the oil phase valve 560, the oil phase electric pump 570, the main oil port 543 of the oil phase reversing valve 540 and the second working oil port 542 of the oil phase reversing valve 540 to enter the lower cavity of the oil phase injector 510, and the oil phase in the upper cavity of the oil phase injector 510 flows into the oil phase main channel 210 through the oil phase injection port 511 of the oil phase injector 510, the oil phase throttling valve 520 and the oil phase first one-way valve 530; the water phase in the water phase container 400 sequentially passes through the water phase valve 660, the water phase electric pump 670, the main water port 643 of the water phase reversing valve 640 and the second working water port 642 of the water phase reversing valve 640 to enter the lower cavity of the water phase injector 610, and the water phase in the upper cavity of the water phase injector 610 flows into the water phase main channel 220 through the water phase injection port 611 of the water phase injector 610, the water phase throttling valve 620 and the water phase first one-way valve 630; during the period, the oil phase injector 510 injects air first and then injects the oil phase, and the water phase injector 610 injects air first and then injects the water phase; finally, the microscope camera 700 takes a photograph, the industrial personal computer 1200 image-recognizes the photograph, and when it is determined that the droplet is defective, the droplet is flowed into the waste liquid container 1000, and when it is determined that the droplet is defective, the droplet is rapidly flowed into the collection container 1100 by the action of the droplet motor pump 920.
Compared with the prior art, the microfluidic device in the first embodiment can automatically generate liquid drops, and the injector is also used for injecting liquid, but the device can automatically identify qualified liquid drops and unqualified liquid drops and automatically separate the qualified liquid drops and the unqualified liquid drops, so that the product quality is improved. And the injector discharges all air and then discharges the liquid phase, so that qualified liquid drops and unqualified liquid drops can be treated uniformly, the unqualified liquid drops are prevented from being doped in the qualified liquid drops, and the product quality is further improved. In addition, feedback information is given by means of data collected by the microscope head, so that the flow velocity of the water phase and the oil phase is operated, and full-automatic preparation of the liquid drops can be realized.
In addition, it should be emphasized that the microfluidic device described in this embodiment can be not only an apparatus for generating oil-in-water droplets, but also an apparatus for generating water-in-oil droplets, and only the inner conduit 232 of the microfluidic chip 200 is connected to the aqueous phase injector 610, and the outer conduit 231 of the microfluidic chip 200 is connected to the oil phase injector 510.
Example two
A microfluidic device, see fig. 8, which differs from the microfluidic device described in the first embodiment by: a transparent chip 1400 is arranged in the shooting range of the microscope camera 700, a transparent pipeline is arranged in the transparent chip 1400, and two ends of the transparent pipeline are respectively connected with the two-position three-way electromagnetic valve 910 and the waste liquid container 1000; specifically, the transparent chip 1400 is generated by micro-nano 3D printing, and the transparent chip 1400 is made of a transparent material; when the industrial personal computer 1200 recognizes that droplets meeting the requirements are output in the transparent pipeline, the two-position three-way electromagnetic valve 910 is reversed, so that the subsequent droplets flow into the collection container 1100.
Compared with the microfluidic device described in the first embodiment, the microfluidic device described in the second embodiment performs droplet inversion after detecting that qualified droplets flow to the waste liquid container 1000, which is beneficial to improving the quality of products.
EXAMPLE III
A microfluidic device, see fig. 9, 10, 11, which differs from the microfluidic device described in the first embodiment by: the droplet generation pipeline 230 further includes a secondary pipeline 233, the liquid outlet end of the outer pipeline 231 is disposed in the secondary pipeline 233, the liquid outlet direction of the outer pipeline 231 is parallel to the liquid flowing direction in the secondary pipeline 233, the outer wall of the liquid outlet end of the outer pipeline 231 is tapered, the liquid outlet end of the secondary pipeline 233 is connected to the liquid outlet pipeline 240, the liquid phase sent by the secondary pipeline 233 is the same as the liquid phase sent by the inner pipeline 232, the secondary pipeline 233 also sends the oil phase, the oil phase in the secondary pipeline 233 and the oil phase in the inner pipeline 232 may be provided by the same oil phase injection device 500, or may be provided by two independent oil phase injection devices 500, which is not limited herein.
It should be noted that the microfluidic device described in this embodiment is not only a device for generating water-in-oil and oil-in-water droplets, but also a device for generating water-in-oil and water-in-water droplets, and only the inner pipe 232 of the microfluidic chip 200 is connected to the aqueous phase injector 610, the outer pipe 231 of the microfluidic chip 200 is connected to the oil phase injector 510, and the secondary pipe 233 of the microfluidic chip 200 is connected to the aqueous phase injector 610.
In contrast to the microfluidic device described in the first embodiment, the microfluidic device described in the third embodiment can generate not only a single-layer droplet but also a double-layer droplet.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (10)
1. A microfluidic device, characterized in that it comprises:
a microfluidic chip (200) for generating droplets;
the oil phase injection device (500) comprises an oil phase injector (510) for injecting oil phase into the microfluidic chip (200), the top end of the oil phase injector (510) is provided with an oil phase injection port (511) communicated with the microfluidic chip (200), and an oil phase piston (512) moving to the oil phase injection port (511) along the inner wall of the oil phase injector (510) is arranged in the oil phase injector (510);
the water phase injection device (600) comprises a water phase injector (610) for injecting a water phase into the microfluidic chip (200), the top end of the water phase injector (610) is provided with a water phase injection port (611) communicated with the microfluidic chip (200), and a water phase piston (612) moving to the water phase injection port (611) along the inner wall of the water phase injector (610) is arranged in the water phase injector (610);
and the liquid separation device (900) is used for controlling the flow direction of the liquid drops, is connected with an outlet of the microfluidic chip (200), and is provided with a first discharge hole for qualified liquid drops to flow out and a second discharge hole for unqualified liquid drops to flow out.
2. The microfluidic device according to claim 1, wherein: the liquid separating device (900) comprises a two-position three-way electromagnetic valve (910), and a liquid inlet communicated with an outlet of the microfluidic chip (200), a first discharge hole for qualified liquid drops to flow out and a second discharge hole for unqualified liquid drops to flow out are arranged on the two-position three-way electromagnetic valve (910).
3. The microfluidic device according to claim 2, wherein: an output port for discharging qualified liquid drops on the two-position three-way electromagnetic valve (910) is connected with a liquid drop electric pump (920).
4. The microfluidic device according to claim 1, wherein: still including being used for shooing microscope camera (700), industrial computer (1200) of micro-fluidic chip (200) liquid droplet generating conditions, industrial computer (1200) respectively with oil phase injection device (500), aqueous phase injection device (600), microscope camera (700) electric connection, industrial computer (1200) have integrateed the video identification technique, industrial computer (1200) can judge the size that generates the liquid droplet in real time to give feedback information in order to control the velocity of flow of oil phase or aqueous phase.
5. The microfluidic device according to claim 4, wherein: the oil phase is added with a first edible pigment, the water phase is added with a second edible pigment, the color of the first edible pigment is different from that of the second edible pigment, the color of the first edible pigment is green, and the color of the second edible pigment is red.
6. The microfluidic device according to claim 4, wherein: the microscope further comprises a transparent chip (1400) with a transparent pipeline, wherein the transparent chip (1400) is located in the shooting range of the microscope camera (700), and one end of the transparent pipeline is connected with the second discharge hole.
7. The microfluidic device according to claim 1, wherein: also included is an ultrasonic module (1300), the ultrasonic module (1300) for eliminating air in the oil phase injector (510) or for discharging air in the water phase injector (610).
8. The microfluidic device according to claim 1, wherein:
the oil phase injector (510) is vertically arranged, and the oil phase piston (512) vertically moves up and down along the inner wall of the oil phase injector (510);
the water phase injector (610) is vertically arranged, and the water phase piston (612) vertically moves up and down along the inner wall of the water phase injector (610);
also comprises an oil phase container (300) and a water phase container (400);
the oil phase injection device (500) further comprises an oil phase throttling valve (520), an oil phase first one-way valve (530), an oil phase reversing valve (540), an oil phase valve (560), an oil phase electric pump (570), an oil phase second one-way valve (550), an oil phase third one-way valve (580) and an oil phase overflow valve (590), wherein an oil phase injection port (511) of the oil phase injector (510) is communicated to the micro-fluidic chip (200) through the oil phase throttling valve (520) and the oil phase first one-way valve (530) in sequence, a main oil port (543), a first working oil port (541), a second working oil port (542) and an oil return port (544) are arranged on the oil phase reversing valve (540), the oil phase container (300) is connected to the oil phase injector (510) through the oil phase valve (560), the oil phase electric pump (570), the main oil port (543), the first working oil port (541) and, the oil phase injector (510) is connected to the oil phase container (300) through a second working oil port (542), an oil return port (544) and an oil phase third one-way valve (580) in sequence to recover the oil phase, and an oil phase overflow valve (590) is arranged between the outlet of the oil phase electric pump (570) and the outlet of the oil phase third one-way valve (580);
the water phase injection device (600) further comprises a water phase throttling valve (620), a water phase first one-way valve (630), a water phase reversing valve (640), a water phase valve (660), a water phase electric pump (670), a water phase second one-way valve (650), a water phase third one-way valve (680) and a water phase overflow valve (690), a water phase injection port (611) of the water phase injector (610) is communicated to the micro-fluidic chip (200) through the water phase throttling valve (620) and the water phase first one-way valve (630) in sequence, a main water port (643), a first working water port (641), a second working water port (642) and a water return port (644) are arranged on the water phase reversing valve (640), the water phase container (400) is connected to the water phase injector (610) to push the piston to move through the water phase valve (660), the water phase electric pump (670), the main water port (643), the first working, the water phase injector (610) is connected to the water phase container (400) through a second working water port (642), a water return port (644) and a water phase third one-way valve (680) in sequence to recover the water phase, and a water phase overflow valve (690) is arranged between an outlet of the water phase electric pump (670) and an outlet of the water phase third one-way valve (680).
9. The microfluidic device according to claim 1, wherein: the micro-fluidic chip (200) is generated by micro-nano 3D printing and is made of transparent materials, a liquid outlet pipeline (240) and at least 5 liquid drop generating pipelines (230) communicated with the liquid outlet pipeline (240) are arranged in the micro-fluidic chip (200), the droplet generation conduit (230) comprises an outer conduit (231), an inner conduit (232), the liquid outlet end of the inner pipeline (232) is arranged in the outer pipeline (231), the liquid outlet direction of the inner pipeline (232) is parallel to the liquid flowing direction in the outer pipeline (231), the outer pipe wall of the liquid outlet end of the inner pipe (232) is conical, the liquid outlet end of the outer pipe (231) is connected with the liquid outlet pipe (240), the outer pipe (231) is used for feeding either one of the liquid phases of the oil phase and the water phase, and correspondingly, the inner pipe (232) is used for feeding the other one of the liquid phases of the oil phase and the water phase.
10. The microfluidic device according to claim 9, wherein: the liquid drop generating pipeline (230) further comprises a secondary pipeline (233), the liquid outlet end of the outer pipeline (231) is arranged in the secondary pipeline (233), the liquid outlet direction of the outer pipeline (231) is parallel to the liquid flowing direction in the secondary pipeline (233), the outer pipe wall of the liquid outlet end of the outer pipeline (231) is conical, the liquid outlet end of the secondary pipeline (233) is connected with the liquid outlet pipeline (240), and the liquid phase sent by the secondary pipeline (233) is the same as the liquid phase sent by the inner pipeline (232).
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| CN111330657B (en) * | 2020-03-06 | 2021-12-31 | 上海材料研究所 | Micro-fluidic device based on phased array ultrasonic transducer |
| CN112871096A (en) * | 2021-01-12 | 2021-06-01 | 厦门柯迪森科技有限公司 | Multi-mode micro-fluidic device |
| CN113092796A (en) * | 2021-04-02 | 2021-07-09 | 浙江大学 | Accurate sample introduction system for water phase and oil phase |
| CN113248338A (en) * | 2021-05-12 | 2021-08-13 | 中北大学 | Preparation system and method of HMX-based energetic microspheres based on droplet microfluidic technology |
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