CN110679955A - Giant salamander active peptide nutritional composition capable of improving intestinal function - Google Patents
Giant salamander active peptide nutritional composition capable of improving intestinal function Download PDFInfo
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Images
Classifications
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
- A23L21/25—Honey; Honey substitutes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/198—Dry unshaped finely divided cereal products, not provided for in groups A23L7/117 - A23L7/196 and A23L29/00, e.g. meal, flour, powder, dried cereal creams or extracts
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Nutrition Science (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention discloses a giant salamander active peptide nutritional composition capable of improving intestinal functions, which comprises the following raw materials, by mass, 20 ~ 70 parts of giant salamander active peptide, 0.5 ~ 10 part of honey powder, 0.5 ~ 20 part of hawthorn extract, 0.1 ~ 25 part of inulin, 1 ~ 10 parts of green tea powder and 0.5 ~ 20 part of barley seedling powder.
Description
Technical Field
The invention belongs to the field of functional foods, and particularly relates to a giant salamander active peptide nutritional composition capable of improving intestinal functions.
Background
The intestinal tract refers to the digestive tract from the stomach pylorus to the anus. The intestinal tract of a mammal comprises three large sections, namely a small intestine, a large intestine and a rectum, wherein the small intestine mainly has the functions of food digestion and nutrition absorption, bacteria are resisted by depending on beneficial bacteria in the intestine while nutrition is absorbed, so that the bacteria are prevented from invading human body circulation, and the large intestine mainly has the functions of concentrating food residues, forming excrement and then discharging the excrement out of a body through a rectum and an anus. Therefore, the intestinal tract is an important digestive organ and an immune organ of a human body, so that the functions of maintaining the number of flora in the intestinal tract, improving the occupation ratio of beneficial bacteria, keeping normal peristalsis of the intestinal tract, clearing intestinal toxins (defecation) and the like are basically guaranteed for the health of the human body. Otherwise, intestinal diseases such as intestinal cancer, appendicitis, hemorrhoid, enteritis, constipation, body odor, halitosis, etc., and health problems such as obesity, skin darkening and roughness, etc. can be caused.
The invention discloses a nutritional composition for intestinal tract cleaning and intestinal tract toxin expelling, which is prepared from the following raw materials in percentage by mass, 23.79 to 53976.12 sugar alcohols, 4.76 to ~ 52.33 fructo-oligosaccharides, 0.24 to ~.28 hawthorn extracts, 0.24 to ~.28 honey powder and 0.1 to ~.88 inulin, and simultaneously carries out comparative experiments on constipation people in aspects of gas discharge feeling, defecation frequency and other body senses, so that the nutritional composition has the effects of promoting intestinal tract movement, intestinal tract cleaning and intestinal tract toxin expelling.
Chinese invention patent number ZL201610314751.X discloses a giant salamander active peptide and application, wherein the giant salamander active peptide is prepared according to the following steps:
homogenizing giant salamander meat, adding 1 ~ 3-time volume of 0.01 ~ 0.1.1M phosphate buffer solution with the pH value of 7 ~ 8 and 0.1 ~ 1% of complex enzyme with the weight ratio of the homogenate to carry out enzymolysis for 12 ~ 48 h, wherein the complex enzyme is marine alkaline protease and papain which are mixed according to the activity unit ratio of 7: 3;
centrifuging to obtain supernatant, separating with trypsin fixed ultrafiltration membrane separator with molecular weight cutoff below 4000 Da, and collecting liquid penetrating through the ultrafiltration membrane;
collecting chromatographic products by Sephadex LH-20 molecular sieve chromatographic column according to 1ml per tube, and freeze-drying the 30 th 30 ~ 36 th tube chromatographic product;
d. c, preparing the freeze-dried product obtained in the step c into a deionized water solution of 10 ~ 50mg/ml, centrifuging for 10 min at 8000 rpm and 4 ℃, and filtering by using a 0.22 mu m water film to obtain a supernatant;
e, preparing a 5% methanol buffer solution by using ddH2O, performing suction filtration through a water film with the aperture of 0.45 mu m, performing ultrasonic degassing to obtain a mobile phase A of the high performance liquid chromatography column, preparing a 95% methanol buffer solution by using ddH2O, performing suction filtration through an oil film with the aperture of 0.45 mu m, and performing ultrasonic degassing to obtain a mobile phase B of the high performance liquid chromatography column, performing gradient elution by using the mobile phase A and the mobile phase B under the conditions that the flow rate is 1ml/min, the elution gradient distribution is 0 ~ 20min, the mobile phase A is 100%, the mobile phase B is 20.5 ~ 40min, the mobile phase A is 50%, the mobile phase B is 50%, the mobile phase B is 40.5 ~ 60min, and the mobile phase B is 100%, injecting a supernatant into a sample inlet of a C18 column high performance liquid chromatograph, and collecting an absorption peak with;
and f, volatilizing the methanol in the collected matter, and carrying out vacuum freeze drying to obtain the giant salamander peptide product.
The prepared giant salamander bioactive peptide has the effect of promoting skin fibroblast proliferation.
So far, no report about the compatibility of giant salamander active peptide and other components to maintain the health of intestinal microbial flora and clear enterotoxin is disclosed.
Disclosure of Invention
The invention aims to solve the technical problems in the prior art and provides a giant salamander active peptide nutritional composition for improving intestinal functions.
The technical scheme is that the giant salamander active peptide nutritional composition capable of improving intestinal functions is characterized by comprising the following raw materials, by mass, 20 ~ 70 of giant salamander active peptide, 0.5 ~ 10 of honey powder, 0.5 ~ 20 of hawthorn extract, 0.1 ~ 25 of inulin, 1 ~ 10 of green tea powder and 0.5 ~ 20 of barley seedling powder.
The components of the invention have synergistic effect, can effectively maintain the number of the flora in the intestinal tract, improve the proportion of beneficial bacteria, keep the normal peristalsis of the intestinal tract, recover the intestinal motility and discharge the intestinal toxins, and can effectively improve the intestinal function and ensure the health of the intestinal tract.
Drawings
FIG. 1 is a schematic diagram of the population composition of the present invention comparing with a database, performing species classification of OTU and performing histogram analysis on each sample on a phylum level.
FIG. 2 is a schematic diagram of the flora composition of the embodiment of the present invention comparing with a database, performing species classification on OTU and performing histogram analysis on each sample on class level.
FIG. 3 is a schematic diagram of the flora composition of the embodiment of the present invention comparing with the database, performing species classification on OTU and performing histogram analysis on each sample on a target level.
FIG. 4 is a schematic diagram of the flora composition of the embodiment of the present invention comparing with the database, classifying OTU species and performing histogram analysis on each sample on the family level.
FIG. 5 is a schematic diagram of the flora composition of the embodiment of the present invention comparing with the database, performing species classification on OTU and performing histogram analysis on each sample on genus level.
Detailed Description
Example 1:
the giant salamander active peptide nutritional composition capable of improving intestinal functions, provided by the invention, comprises the following raw materials in percentage by mass: 5kg of giant salamander active peptide, 0.5kg of honey powder, 1kg of hawthorn extract, 1.5kg of inulin, 0.5kg of green tea powder and 1kg of barley seedling powder, and mixing the raw materials. Can be used as additive of candy, meal, solid beverage, liquid beverage or intestinal tract lavage toxin expelling substance.
The giant salamander active peptide is prepared according to a method disclosed by Chinese invention patent number ZL201610314751.X, giant salamander active peptide and application.
The honey powder is prepared according to a literature method (Yuanyun, etc. the spray drying process research of the honey powder, Chinese food additives, 206, (12): 139-142); the hawthorn extract is prepared according to a literature method (Liuchuyu, and the like, preparation process research of cordyceps militaris and hawthorn extract solid beverage, food research and development, 2018, 39 (24): 77-82), and inulin is purchased from Alibara; the barley grass powder is prepared according to a literature method (Qionghui, etc., barley grass powder nutrient component analysis and evaluation, modern traditional Chinese medicine research and practice, 2014, 28 (3): 55-57), and green tea powder is purchased in the east of Beijing.
Example 2:
the giant salamander active peptide nutritional composition capable of improving intestinal functions, provided by the invention, comprises the following raw materials in percentage by mass: 20kg of giant salamander active peptide, 0.5kg of honey powder, 0.5kg of hawthorn extract, 0.1kg of inulin, 1kg of green tea powder and 0.5kg of barley seedling powder, and mixing the raw materials. Can be used as additive of candy, meal, solid beverage, liquid beverage or intestinal tract lavage toxin expelling substance.
The starting materials used were from the same sources as in example 1.
Example 3:
the giant salamander active peptide nutritional composition capable of improving intestinal functions, provided by the invention, comprises the following raw materials in percentage by mass: 7kg of giant salamander active peptide, 1kg of honey powder, 2kg of hawthorn extract, 2.5kg of inulin, 1kg of green tea powder and 2kg of barley seedling powder, and mixing the raw materials. Can be used as additive of candy, meal, solid beverage, liquid beverage or intestinal tract lavage toxin expelling substance.
The starting materials used were from the same sources as in example 1.
Examples of the experiments
Firstly, the influence of the embodiment of the invention on the intestinal flora of mice is researched by utilizing a high-throughput sequencing technology
30 SPF-grade Kunming mice (male) were divided into 3 groups, normal (ads.c.), experimental (ads.m.) and experimental (ads.t.) groups, respectively.
Normal groups were injected subcutaneously daily with saline (0.5 mL/D), and the other two groups were injected subcutaneously daily with an equivalent amount (0.5 mL/D) of 5% D-galactose (saline formulation).
The normal group is gavaged with 200 mg/(kg.d) of normal saline every day, the experiment group is gavaged with 200 mg/(kg.d) of composition aqueous solution every day, the composition is prepared by other raw materials except the giant salamander active peptide in the embodiment 1 of the invention according to the mass ratio, and the experiment group is gavaged with 200 mg/(kg.d) of the nutritional composition aqueous solution in the embodiment 1 of the invention every day. The aqueous composition of one set of experiments was at the same concentration as the aqueous nutritional composition of example 1 of the present invention of the two sets of experiments.
The gavage was continued for 6 weeks, and 24h after the last gavage, the mouse feces were collected and immediately frozen in liquid nitrogen.
The V4 universal primer is adopted to carry out PCR amplification and sequencing on the V4 region of the 16S rRNA gene of the mouse feces. Removing connector pollution reads, reads containing N and low-complexity reads by adopting a window mass removal method, merging and establishing a library by using a barcode, splitting the sample by using the barcode, and analyzing the species composition and abundance of the sample, namely analyzing OTU and abundance thereof (OTU clustering, OTU Wien, OTU PCA analysis and OTU Rank curve), and analyzing the species and abundance thereof; sample complexity analysis, namely single sample complexity (Alpha diversity, Alpha diversity dilution curve chart, Alpha diversity aggregate chart), complexity comparison analysis among samples (Beta analysis and the like), significant difference factor among sample groups and colony difference analysis among LEFSe groups; and (3) performing function prediction and difference analysis on the 16S sequencing sample.
The results are shown in table 1, fig. 2, fig. 3, fig. 4, and fig. 5.
Alpha diversity statistics of the samples of Table 1
| Sample name | OTU | shannon | simpson | chao1 | ACE | goods_coverage | PD_whole_tree |
| ADS.C.1 | 560 | 4.687 | 0.897 | 628.043 | 648.281 | 0.998 | 44.267 |
| ADS.C.2 | 727 | 4.208 | 0.805 | 839.152 | 843.018 | 0.998 | 57.581 |
| ADS.C.3 | 582 | 4.176 | 0.77 | 660.269 | 666.368 | 0.998 | 42.431 |
| ADS.C.4 | 593 | 3.812 | 0.741 | 656.917 | 680.111 | 0.998 | 50.546 |
| ADS.C.5 | 1137 | 6.453 | 0.942 | 1209.065 | 1220.064 | 0.998 | 87.048 |
| ADS.C.6 | 601 | 6.073 | 0.964 | 659.958 | 666.505 | 0.999 | 46.154 |
| ADS.C.7 | 727 | 5.549 | 0.936 | 780.512 | 808.866 | 0.998 | 55.2 |
| ADS.C.8 | 661 | 5.016 | 0.927 | 726.724 | 731.285 | 0.998 | 54.203 |
| ADS.C.9 | 566 | 5.81 | 0.959 | 623.558 | 639.526 | 0.999 | 45.757 |
| ADS.C.10 | 576 | 5.422 | 0.925 | 607.281 | 628.079 | 0.999 | 46.293 |
| ADS.M.1 | 537 | 4.609 | 0.901 | 623.032 | 641.025 | 0.998 | 43.006 |
| ADS.M.2 | 736 | 4.849 | 0.888 | 859.58 | 873.028 | 0.997 | 59.056 |
| ADS.M.3 | 909 | 6.808 | 0.975 | 969.047 | 985.268 | 0.998 | 64.678 |
| ADS.M.4 | 667 | 5.23 | 0.921 | 759.243 | 785.672 | 0.998 | 48.489 |
| ADS.M.5 | 589 | 5.943 | 0.958 | 642.963 | 658.022 | 0.999 | 41.136 |
| ADS.M.6 | 625 | 6.161 | 0.957 | 670.535 | 685.571 | 0.999 | 42.884 |
| ADS.M.7 | 879 | 5.107 | 0.881 | 973.448 | 993.955 | 0.998 | 67.318 |
| ADS.M.8 | 761 | 6.368 | 0.975 | 840.342 | 849.645 | 0.998 | 58.474 |
| ADS.M.9 | 647 | 5.273 | 0.897 | 712.917 | 726.117 | 0.998 | 45.592 |
| ADS.M.10 | 765 | 6.182 | 0.967 | 840.271 | 870.086 | 0.998 | 56.124 |
| ADS.T.1 | 1403 | 7.056 | 0.966 | 1478.019 | 1471.336 | 0.998 | 104.037 |
| ADS.T.2 | 1114 | 6.556 | 0.963 | 1211.55 | 1220.532 | 0.997 | 86.647 |
| ADS.T.3 | 766 | 6.436 | 0.975 | 866.385 | 873.266 | 0.998 | 59.312 |
| ADS.T.4 | 612 | 6.272 | 0.973 | 657.868 | 665.41 | 0.999 | 41.437 |
| ADS.T.5 | 778 | 4.823 | 0.856 | 850.007 | 874.343 | 0.998 | 59.289 |
| ADS.T.6 | 723 | 4.792 | 0.882 | 805.409 | 832.988 | 0.998 | 54.707 |
| ADS.T.7 | 602 | 5.462 | 0.944 | 663.437 | 684.564 | 0.998 | 44.385 |
| ADS.T.8 | 611 | 4.998 | 0.896 | 680.515 | 700.233 | 0.998 | 50.209 |
| ADS.T.9 | 866 | 6.741 | 0.976 | 955.632 | 944.965 | 0.998 | 62.07 |
| ADS.T.10 | 770 | 6.072 | 0.948 | 829.509 | 845.877 | 0.998 | 58.845 |
The Alpha diversity analysis result shows that the flora diversity of the feces of two experimental groups of mice is the highest, the nutritional composition has a regulating effect on the intestinal flora of the mice, and the effect is the most prominent. At the phylum level (fig. 1), Firmicutes decreased in the experimental two groups, Firmicutes (Firmicutes) could help polysaccharide fermentation in the intestine, which indicates that the groups involved in sugar fermentation in the intestinal flora of the experimental two groups of mice decreased, and the nutritional composition of the present example helps to prevent obesity. At the class level (fig. 2), bacillus is a class of firmicutes, which contains many bacteria associated with adverse intestinal conditions, such as streptococcus, staphylococcus, enterococcus, and from experimental data, it can be seen that the proportion of bacillus in the experimental two groups is significantly reduced, indicating that the nutritional composition of the present invention can significantly improve the adverse environment of the intestinal tract of mice. At the objective level (fig. 3), the experimental two groups of Lactobacillales (Lactobacillales) were the most, Bacteroidales (Bacteroidales), Clostridiales (Clostridiales) were the least, indicating that the nutritional compositions of the present examples are able to selectively inhibit the growth of harmful bacteria and optimize the proportion of intestinal flora. At the family level (fig. 4), two experimental groups of veillonellaceae (Erysipelotrichaceae) have the highest proportion, and veillonella is beneficial and harmless to human bodies and can enhance the immunity of the respiratory system and the digestive system of the human bodies. On the subordinate level (fig. 5), the highest proportion is lactobacillus, and the intestinal flora of the experimental two groups of mice is the most abundant, which is beneficial to the development of the intestinal flora towards the health direction.
Second, the effect of the embodiment of the invention on constipation people
20 constipation people who defecate less than 3 times per week (excluding other organ diseases, systemic diseases and constipation caused by drug factors) are randomly divided into an experimental group and an experimental group, and each group takes 20 g of health care product 30min after lunch. The health care product taken by the experimental group is prepared by the raw materials except the giant salamander active peptide in the embodiment 1 of the invention according to the mass ratio, and the health care product taken by the experimental group is the embodiment 1 of the invention. The results are shown in Table 1.
TABLE 1
| Group of | Feeling of exhaust | Number of defecation | Other body sensations |
| Two groups of experiments | Has obvious feeling of exhausting after eating for 2-3h | The number of defecations is increased by 1-3 times for 10 people | Comfortable and relaxing intestinal tract |
| Experiment group | Has slight feeling of exhaust after eating for 2-3h | Only two people increase the defecation times for 1 time | Is free of |
As can be seen from Table 1, the inventive example 2 ~ 3 had a sensation of exsufflation after eating it for 3 hours, and 100% of the members of the experimental group were able to increase defecation 1-3 times, indicating that the inventive example is helpful for intestinal motility, excretion of intestinal toxins, and maintenance of intestinal health.
Claims (1)
1. A giant salamander active peptide nutritional composition capable of improving intestinal functions is characterized by comprising the following raw materials, by mass, 20 ~ 70 parts of giant salamander active peptide, 0.5 ~ 10 parts of honey powder, 0.5 ~ 20 parts of hawthorn extract, 0.1 ~ 25 parts of inulin, 1 ~ 10 parts of green tea powder and 0.5 ~ 20 parts of barley seedling powder.
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| US20180021399A1 (en) * | 2016-07-21 | 2018-01-25 | Infinitus (China) Company Ltd. | Composition and use thereof in manufacture of product for improving intestinal function |
| CN108239583A (en) * | 2018-04-02 | 2018-07-03 | 张家界金驰天问农业科技有限公司 | Giant salamander active peptide grape wine |
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| US20120269865A1 (en) * | 2009-11-12 | 2012-10-25 | Nestec S.A. | Nutritional composition for promoting gut microbiota balance and health |
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| CN106107397A (en) * | 2016-06-27 | 2016-11-16 | 张博 | A kind of Andrias davidianus Blanchard protein peptide powder solid beverage formula and preparation method thereof |
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