CN110376265A - A method of detection ascorbic acid - Google Patents
A method of detection ascorbic acid Download PDFInfo
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- CN110376265A CN110376265A CN201910751824.5A CN201910751824A CN110376265A CN 110376265 A CN110376265 A CN 110376265A CN 201910751824 A CN201910751824 A CN 201910751824A CN 110376265 A CN110376265 A CN 110376265A
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- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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Abstract
The invention discloses a kind of methods for detecting ascorbic acid, are working electrode by glass-carbon electrode, and saturated calomel electrode is reference electrode, and platinum filament is to be put into the three-electrode system of electrode containing 1.00 × 10‑3The L-lysine of mol/L, 3.00 × 10‑4In the solution of mol/L silver nitrate, 0.05mol/L nitric acid and 0.10mol/L potassium nitrate, in -0.4~2.6V potential range, 5s is stood, Ag doping polylysine modified electrode is obtained using 10 circle of cyclic voltammetry scanning with the scanning speed of 60mV/s；With the modified electrode, in the potential range of -0.6~0.8V, the time of repose of 14s under the scanning speed of 160mV/s, uses ascorbic acid solution of the cyclic voltammetry using the phosphate buffer solution of pH=3.0 as supporting electrolyte.The result shows that the oxidation peak current of ascorbic acid and its concentration are 1.00 × 10‑4~5.00 × 10‑3It is in good linear relationship within the scope of mol/L, detection is limited to 3.00 × 10‑5mol/L.The present invention can be used for the Sensitive Detection of ascorbic acid, have the characteristics that accuracy is high, selectivity is good, simple and easy, low in cost, finding speed is fast.
Description
Technical field
The invention belongs to electrochemical technology fields, and in particular to a method of detection ascorbic acid.
Background technique
Ascorbic acid also known as vitamin C (Vitamin C) are clear crystal, sour, soluble easily in water.It is that high spirit is long
The required nutriment of class animal and other a small number of biologies, plays a very important role in vivo, can be as a kind of anti-
Oxidant eliminates threat of the free radical for body；It promotes the shape of collagen in tissue in the form of coenzyme again simultaneously
At participating in the metabolism of substance in vivo.But vitamin C, cannot be in body as a kind of indispensable nutriment of human body
It is inside automatically synthesized, can only be absorbed from the external world.It is widely present in food, animals and plants body fluid and tissue, especially in Kiwi berry, tangerine
Content in the fresh vegetables and fruit such as son, tomato, capsicum is extremely abundant.When the vitamin C of excess intake in human body, suffer from pain
The probability of the diseases such as wind, calculus will increase；If when opposite hypovitaminosis C, the intracorporal cell connection of people will occur obstacle into
And a variety of diseases such as cause scurvy and hypoimmunity.So during daily trophic analysis and certain medicals diagnosis on disease,
The height of ascorbic acid content is often used as one of important indicator.Therefore the analysis side of good effective measurement ascorbic acid is established
Method daily life, health care and in terms of have important researching value and realistic meaning.
The method of measurement ascorbic acid content mainly has a potentiometric titration at present, stripping voltammetry, Extractive catalytic spectrophotometry,
Ultraviolet spectrophotometry, high performance liquid chromatography etc..The operating procedure of the above measuring method is relatively complicated, expends the time
Long, using expensive equipment, analysis cost is high, therefore is unfavorable for preferably quickly being analyzed.Therefore, it establishes a kind of simple easy
The method fast, low in cost, that amount of reagent is small of row, high sensitivity, finding speed has a very important significance.
Summary of the invention
It in view of the deficiencies of the prior art, should the purpose of the present invention is to provide a kind of method for detecting ascorbic acid
Detection method is selectively good, and high sensitivity is simple and easy, and finding speed is fast, low in cost, so as to simpler convenient and fast
Ascorbic acid is detected, provides better measuring means for daily life, health care and medicine etc..
In order to achieve the above objectives, the invention adopts the following technical scheme:
A method of detection ascorbic acid, the various parameters that this method is related to are optimal values, rather than particular value, tool
Steps are as follows for body:
S1: it prepares Ag doping polylysine modified electrode: being working electrode by glass-carbon electrode, saturated calomel electrode is ginseng
Than electrode, platinum filament is to be put into the three-electrode system of electrode containing 1.00 × 10-3The L-lysine of mol/L, 3.00 × 10- 4It is quiet in -0.4~2.6V potential range in the solution of mol/L silver nitrate, 0.05mol/L nitric acid and 0.10mol/L potassium nitrate
5s is set, with the scanning speed of 60mV/s, using 10 circle of cyclic voltammetry scanning to get the polylysine prepared under optimum condition
Modified electrode；
S2: measurement ascorbic acid: with modified electrode obtained above, in the potential range of -0.6~0.8V, the standing of 14s
Time under the scanning speed of 160mV/s, uses cyclic voltammetry using the phosphate buffer solution of pH=3.0 as supporting
The ascorbic acid solution of electrolyte.
The phosphate buffer solution is by Na3PO4、NaH2PO4、Na2HPO4Or K3PO4、KH2PO4、K2HPO4Composition, respectively
Material concentration is 0.1moL/L, H3PO4Adjusting pH is 3.0.
Testing result show ascorbic acid oxidation peak current and its concentration 1.00 × 10-4~5.00 × 10-3mol/L
It is in good linear relationship in range, detection is limited to 3.00 × 10-5mol/L.Beneficial effects of the present invention:
1) Ag doping polylysine modified electrode of the present invention has the wider range of linearity and lower detection limit, is sweeping
It retouches in current potential -0.6~section 0.8V, there is obvious oxidation peak on Ag doping polylysine modified electrode in ascorbic acid,
Spike potential is 0.188, meanwhile, electric current significantly increases, and Ascorbic Acid has stronger electricity to urge after illustrating silver and L-lysine polymerization
Change effect, significantly improves the selectivity and sensitivity of electrode Ascorbic Acid.
2) detection method is selectively good, and high sensitivity is simple and easy, and finding speed is fast, low in cost, amount of reagent
Small, required detection device is convenient, and testing conditions are simple, is daily life so as to simpler convenient and fast detection ascorbic acid
Living, health care and medicine etc. provide better measuring means.
Detailed description of the invention
Fig. 1 is the cyclic voltammogram of the Ag doping polylysine modified electrode prepared under optimum condition；
Fig. 2 is cyclic voltammetry curve of the ascorbic acid on Different electrodes.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
Embodiment 1 prepares Ag doping polylysine modified electrode
It is working electrode by glass-carbon electrode, saturated calomel electrode is reference electrode, and platinum filament is the three-electrode system to electrode
It is put into containing 1.00 × 10-3The L-lysine of mol/L, 3.00 × 10-4Mol/L silver nitrate, 0.05mol/L nitric acid and
In the solution of 0.10mol/L potassium nitrate, in -0.4~2.6V potential range, 5s is stood, with the scanning speed of 60mV/s, is utilized
10 circle of cyclic voltammetry scanning, obtains Ag doping polylysine modified electrode.
The principle of the Ag doping polylysine modified electrode detection ascorbic acid prepared under above-mentioned optimum condition is as follows:
Fig. 1 be optimum condition under prepare Ag doping polylysine modified electrode cyclic voltammetry curve (1~10 indicate sweep
Retouch 1~10 circle), it is seen that there is more apparent silver-colored oxidation peak in 0.3V or so when starting polymerization, and with
The increase of scanning circle number, response spike potential are basically unchanged, and response peak current is slowly increased and with its width of the increase of scanning circle number
It spends also smaller and smaller.This is because the polymer of electrode surface can gradually tend to the increase of circle number during electrode modification
Completely, polymerization speed slows down, and tends towards stability.
Fig. 2 is cyclic voltammetry curve of the ascorbic acid on Different electrodes, it can be seen from the figure that in same electric potential scanning
In section, ascorbic acid (without apparent oxidation peak on curve a), shows that the catalysis of glass-carbon electrode Ascorbic Acid is made in glass-carbon electrode
With very weak, and (there is apparent oxidation peak in ascorbic acid on curve b), spike potential in Ag doping polylysine modified electrode
It is 0.188, and peak current has apparent increase.It follows that silver and L-lysine polymerization after Ascorbic Acid have it is stronger
Catalytic action significantly improves the selectivity and sensitivity of electrode Ascorbic Acid.
Embodiment 2 directly measures 8.00 × 10-4Mol/L ascorbic acid
The Ag doping polylysine modified electrode that will be prepared by embodiment 1, the phosphate-buffered for being put into pH=3.0 are molten
In liquid, as supporting electrolyte, which is by Na3PO4、NaH2PO4、Na2HPO4Composition, each material concentration are equal
For 0.1moL/L, H is utilized3PO4Adjusting pH is 3.0, after standing 14s, in -0.6~0.8V potential range, using cyclic voltammetry
With the scanning speed of 160mV/s to contain 8.00 × 10-4The ascorbic acid solution of mol/L is scanned measurement, then measures respectively
A series of ascorbic acid solutions containing known concentration draw standard curve.
According to standard curve, acquiring ascorbic acid concentrations using oxidation peak current is 8.112 × 10-4Mol/L, with true value
Relative standard deviation be 1.4%.
The ascorbic acid of 3 Standard Addition Method for Determination unknown concentration of embodiment
According to 2 condition of embodiment, modified electrode prepared by embodiment 1 is placed in containing in unknown concentration ascorbic acid solution
Measurement is added 2.00 × 10 in the solution-3After mol/L ascorbic acid, measure again.
Test result shows that the concentration of ascorbic acid in unknown sample is 4.15 × 10-4Mol/L, standard addition method measure
The rate of recovery is 98.21%.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details.
Claims (3)
1. a kind of method for detecting ascorbic acid, it is characterised in that: Ag doping polylysine modified electrode is used, in pH=
3.0 phosphate buffer solution, the potential range of -0.6~0.8V, the time of repose of 14s under the scanning speed of 160mV/s, are known
Not with detection ascorbic acid；
The Ag doping polylysine modified electrode preparation method: using glass-carbon electrode as working electrode, saturated calomel electrode is
Reference electrode, platinum filament are three-electrode system to be constructed to electrode, and immerse and contain 1.00 × 10-3The L-lysine of mol/L, 3.00 ×
10-4In the mixed solution of mol/L silver nitrate, 0.05mol/L nitric acid and 0.10mol/L potassium nitrate, in -0.4~2.6V current potential model
In enclosing, 5s is stood, Ag doping polylysine is obtained using 10 circle of cyclic voltammetry scanning with the scanning speed of 60mV/s and repairs
Adorn electrode.
2. a kind of method for detecting ascorbic acid according to claim 1, which is characterized in that the phosphate buffer solution
It is by Na3PO4、NaH2PO4、Na2HPO4Or K3PO4、KH2PO4、K2HPO4Composition, each material concentration is 0.1moL/L, H3PO4It adjusts
Saving pH is 3.0.
3. a kind of method for detecting ascorbic acid according to claim 1, which is characterized in that the oxidation peak electricity of ascorbic acid
Stream is with its concentration 1.00 × 10-4~5.00 × 10-3It is in good linear relationship within the scope of mol/L, detection is limited to 3.00 × 10-5mol/L。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112345618A (en) * | 2020-10-29 | 2021-02-09 | 蚌埠学院 | Preparation method of Cu/VB12/GCE modified electrode and application of Cu/VB12/GCE modified electrode in VC detection |
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| CN101587094A (en) * | 2009-05-26 | 2009-11-25 | 上海大学 | Method for concurrent measurement of dopamine and ascorbic acid using glassy carbon electrode |
| CN109298047A (en) * | 2018-10-23 | 2019-02-01 | 大连大学 | A method of ascorbic acid is measured using silver nanowires/nanogold combination electrode |
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2019
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| US20060021881A1 (en) * | 2003-09-30 | 2006-02-02 | Nano-Proprietary, Inc. | Nanobiosensor and carbon nanotube thin film transistors |
| CN101587094A (en) * | 2009-05-26 | 2009-11-25 | 上海大学 | Method for concurrent measurement of dopamine and ascorbic acid using glassy carbon electrode |
| CN109298047A (en) * | 2018-10-23 | 2019-02-01 | 大连大学 | A method of ascorbic acid is measured using silver nanowires/nanogold combination electrode |
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| 胡文娜 等: "Ag／聚Ｌ-甲硫氨酸复合修饰电极的制备及其用于果蔬中维生素Ｃ的测定", 《皖西学院学报》 * |
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| CN112345618A (en) * | 2020-10-29 | 2021-02-09 | 蚌埠学院 | Preparation method of Cu/VB12/GCE modified electrode and application of Cu/VB12/GCE modified electrode in VC detection |
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