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CN110235857B - Method for killing tobacco leaf pink borers in storage by feeding and transporting adult meadowworms with tobacco pink borers as hosts - Google Patents

Method for killing tobacco leaf pink borers in storage by feeding and transporting adult meadowworms with tobacco pink borers as hosts Download PDF

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CN110235857B
CN110235857B CN201910489260.2A CN201910489260A CN110235857B CN 110235857 B CN110235857 B CN 110235857B CN 201910489260 A CN201910489260 A CN 201910489260A CN 110235857 B CN110235857 B CN 110235857B
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tobacco
box
adult
feeding
moth
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金鑫
杨茂发
刁朝强
廖勇
欧后丁
杨洪
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Guizhou University
Guiyang Office of Guizhou Tobacco Corp
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Guiyang Office of Guizhou Tobacco Corp
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
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    • A23K20/105Aliphatic or alicyclic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/111Aromatic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/116Heterocyclic compounds
    • A23K20/137Heterocyclic compounds containing two hetero atoms, of which at least one is nitrogen
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/90Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B1/00Preparation of tobacco on the plantation
    • A24B1/02Arrangements in barns for preparatory treatment of the tobacco, e.g. with devices for drying
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D85/00Containers, packaging elements or packages, specially adapted for particular articles or materials
    • B65D85/50Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage

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Abstract

The invention discloses a method for killing tobacco stem borers in storage by feeding and transporting adult bracon hebetor with the tobacco stem borers as hosts, belonging to the technical field of storage prevention and control of stem borers and moth pests, and comprising the following steps: s1, preparing a feed for the tobacco meal moth; s2, providing a growing environment for the tobacco powder borers and collecting the eggs of the tobacco powder borers at regular time; s3, transferring eggs of the tobacco meal moth to a first feeding box, and adding the feed prepared in the S1 into the first feeding box; s4, transferring the hatched tobacco pink moth larvae into a second rearing box, and putting paired adult 1-generation bracon hebetor into the second rearing box; s5, after eggs, larvae and pupae of the 2-generation braconid mothflies are finally eclosized into adult flies, collecting the adult flies at low temperature, and transferring the adult flies into a transport box for loading; and S6, releasing bees after the boxes to be transported are transferred to a tobacco warehouse, and killing the tobacco powder borers in the tobacco leaves in the warehouse in a parasitic manner.

Description

Method for killing tobacco leaf pink borers in storage by feeding and transporting adult meadowworms with tobacco pink borers as hosts
Technical Field
The invention relates to the technical field of prevention and control of moth pests in storage, in particular to a method for killing tobacco borers in storage by feeding and transporting adult braconid flies of wheat moths by taking the tobacco borers as hosts.
Background
Tobacco pink borer Ephestia elutella (Hubner), belonging to Lepidoterera of Lepidoptera, Pyralidae of Bombycidae, also known as tobacco pink borer, tobacco borer, etc., are widely distributed in countries all over the world and in China (Romeihao and Li Zhengjun, 2010) except Heilongjiang, Jilin, Xinjiang and Tibet (unrefined), have serious occurrences in Yunnan and Guizhou (Gaojiahe et al, 1999), and have distribution and occurrence in cigarette warehouses of Guizhou (Chengxi and Gansu shou, 1996). The larvae have wide feeding habits, not only harm the tobacco leaves in the storage period, but also harm cocoa, wheat and the like, so that the stored tobacco leaves are not broken completely, even only leave leaf veins, the tobacco shredding process requirement is influenced, and the quality of the tobacco leaves can be reduced by 1-2 grades due to insect feces and insect corpses (protein foreign matters) generated in the damaging process (Gong-letter et al, 1995); according to research reports, the loss rate of tobacco leaves in a young pine tobacco factory in Guizhou province in 1986 is as high as 57 percent (Duyan et al, 2000) because of the tobacco powder borer, and the tobacco leaves are damaged together with other storage pests to cause about 3115 million kg of tobacco leaves in China per year. The existing research shows that the bracon hebetor can effectively kill the tobacco meal moth in a parasitic way, thereby achieving the biological control effect; compared with the physical or chemical method for treating the tobacco powder borer, the biological control method has the advantages of small pollution, quick response and high killing degree of the tobacco powder borer in a warehouse, but at present, aiming at how to kill the tobacco powder borer by using the wheat moth bracon in a large batch, domestic research is still in a blank state, and a series of problems in the process from raising a host to storing the tobacco powder borer in the warehouse are solved by the method without a corresponding device or means.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the method for killing the tobacco whiteflies in the warehouse by feeding and transporting the adult mothflies using the tobacco whiteflies as hosts is provided, so as to solve the problem that an effective method for killing the tobacco whiteflies in the warehouse by using the mothflies in batches is not available in China at present.
In order to solve the problems, the invention provides the following technical scheme:
a method for killing tobacco leaf pink borers in storage by feeding and transporting adult wheat moth bracon flies with the tobacco leaf pink borers as hosts comprises the following steps:
s1, preparing an artificial feed for raising the larvae of the tobacco meal moth: the artificial feed comprises the following components in parts by weight: 27-33% of dry corn grit, 36.3-73.3% of dry broken wheat, 5.5-8.5% of yeast powder, 1.2-2.8% of cooked soybean powder, 0-7% of oatmeal, 0.15-0.35% of methyl ni-platinum, 0.10-0.4% of sorbic acid, 0.1-0.3% of moroxydine hydrochloride tablets, 6-8% of glycerol, 5-9% of honey and 2.5-3.5% of water; the preparation method comprises the steps of weighing the materials and then stirring;
s2, transferring the 1-3d eclosion adult tobacco meal moth larvae population into an oviposition box, wherein the environment temperature of the oviposition box is 27-29 ℃, the relative humidity is 70-80%, and the photoperiod is 16L; adding 20-30% of honey water into the spawning box every day, and collecting eggs in the spawning box in a period of 1-3 d;
s3, placing the artificial feed prepared in the S1 into a first feeding box, uniformly scattering the eggs collected in the S2 into the first feeding box, and placing the first feeding box into an environment with the same environmental conditions as the egg laying box;
s4, transferring a certain number of larvae of the tobacco meal moth larvae from the first rearing box to the second rearing box after the eggs of the tobacco meal moth larvae are hatched into larvae, and introducing paired 1-generation bracon hebetor imagoes into the second rearing box; adding 10-70% honey sugar water into the second feeding box every day; placing the second feeding box in an environment with the same environmental conditions as the spawning box; obtaining 2 generations of bracon hebetor after the 1 generation of bracon hebetor parasitizes and spawns the tobacco pink moth larvae;
s5, after the eggs, larvae and pupae of the 2-generation braconid flies in the S4 are finally eclosized into adult flies, the second feeding box is transferred to the environment with the temperature of 2-6 ℃ until the adult flies are anesthetized at low temperature and can not move; transferring the adult braconid flies of the male moths from the second feeding box to a transport box for loading;
s6, transporting the transport box to a warehouse where tobacco leaves are stored, and transferring the moth cocoon bees in the transport box into a bee releasing device; and opening the bee placer for releasing the bees when the tobacco pink borer larvae appear on the tobacco bale, and parasitizing and killing the tobacco pink borer on the tobacco leaves by the adult wasps with the moth braconid.
Preferably, the ratio of the number of the tobacco meal moth larvae to the pair of bracon hebetor larvae in the step S2 is 10: 1.
Preferably, the second feeding box has a cylindrical box body structure with the bottom surface diameter of 10cm and the height of 8 cm.
Preferably, the spawning box used in the step S2 includes a bottle body and a bottle cap arranged on the bottle body, an air hole is formed in the upper end face of the bottle cap, a first gauze used for sealing is arranged in the air hole, an insect inlet is formed in the bottle body below the bottle neck, the insect inlet is sealed by a cotton plug, a round hole is formed in the bottom of the bottle body, a second gauze used for sealing is arranged in the round hole, and an egg collecting disc is arranged below the bottle body.
Preferably, the transportation box used in the step S5 includes a square box body, and an installation groove is arranged on the upper part of the inner wall of the box body; the top cover is arranged on the top of the box body in a drawing way through the mounting groove; a clapboard is arranged in the middle of the box body; the box body is divided into a plurality of cells by the partition boards; the inner wall of each unit cell is provided with a positioning block; the bee storage tank is detachably placed in the box body through the positioning block; more than one drip irrigation main pipe is arranged on the lower bottom end surface of the top cover opposite to the box body; the lower bottom of the drip irrigation main pipe is provided with drippers with the positions and the number corresponding to the unit grids; the water dropper is communicated with the drip irrigation main pipe; one end of the drip irrigation main pipe is connected with the honey water container through a connecting pipe; a nutrient solution pipe communicated with the inside and the outside of the bee storage tank is arranged on the top end surface of the bee storage tank; the adult braconid flies of the wheat moth are placed in the bee storage tank.
Furthermore, the bee storage tank is of a cylindrical structure, the upper top surface of the bee storage tank is of an inwards concave conical surface structure, the outer wall of the lower end of the bee storage tank is provided with external threads, and the positioning blocks are of four column block structures which are symmetrically arranged in a cross manner in the unit cells; and one side of the positioning block, which is connected with the bee storage tank, is of a cambered surface structure; an inner screw section matched with the outer screw section is arranged on the arc surface side of the positioning block connected with the bee storage tank; one side that the nutrition liquid pipe stretches out and stores up bee tank deck portion is the funnel structure, and nutrition liquid pipe stretches into and opens on the pipeline in storing up the bee jar and has the overflow hole, and has wrapped up in the cotton layer in the outside of pipeline.
Furthermore, the four walls of the box body are surrounded by three long plates with the same structure and a short plate; the mounting grooves are arranged on the three long plates and higher than the short plates, and the lower end face of the top cover is also provided with a protective sleeve; the protective sleeve surrounds the drip irrigation main pipe along the circumferential direction of the drip irrigation main pipe; and a through hole is arranged at the position of the protective sleeve opposite to the dripper; the upper top of the short plate is provided with a step; the step surface of the step is arranged towards the outside of the box body; the top cover is of an L-shaped plate structure, and one end of the L-shaped plate is lapped on the step; connecting blocks are respectively arranged on the outer end faces of the L-shaped plate and the step; the two connecting blocks are provided with pin holes and are fixed through fixing pins.
The invention has the beneficial effects that:
the beneficial effects of the invention are shown in the following aspects: 1. by configuring the feed of the tobacco meal moth and providing the environmental conditions required by the growth and oviposition of the tobacco meal moth cocoon, the problem that the batch cultivation cannot be realized because a proper oviposition host is difficult to find in the process of cultivating the wheat moth cocoon bee is effectively solved; 2. reliable environmental conditions are provided for the normal mating and oviposition of 1 generation of bracon hebetor, and the parasitism rate of the larvae of the tobacco meal moth as hosts is improved, so that the number of the 2 generation of bracon hebetor is increased; 3. the method for transferring, packaging and transporting the adult braconid flies of the wheat moth can improve the life cycle of the braconid flies of the wheat moth, thereby enabling customers in different areas to be used. On the other hand, the invention also provides an oviposition box convenient for the tobacco meal moth to lay eggs and a transportation box capable of supplementing nutrition to adult braconid flies during transportation, so as to solve the problems of inconvenient collection and low survival rate of the adult braconid flies during specific implementation.
Drawings
FIG. 1 is a schematic diagram of the structure of an ovipositing cassette of the present invention;
FIG. 2 is a schematic view of the construction of the transport box of the present invention;
FIG. 3 is a top view of the lower cassette of FIG. 2;
FIG. 4 is a bottom plan view of the upper header of FIG. 2;
FIG. 5 is a schematic view of the construction of the bee storage tank of the present invention;
description of reference numerals: 1. the insect pest killing device comprises a bottle body, a bottle cover, a bottle body 3, air holes 4, a first gauze, a first insect pest inlet 5, an insect pest inlet 6 and a cotton plug; 7. a second gauze 8, an egg collecting plate;
1A, a box body, 2A, the mounting groove, 3A, the baffle, 4A, the locating piece, 5A, store up the bee jar, 6A, drip irrigation the person in charge, 7A, the honey water container, 8A, the connecting block, 11A, the top cap, 12A, the long slab, 13A, the short slab, 14A, the cell, 31A, horizontal baffle, 32A, longitudinal baffle, 51A, the nutrient solution pipe, 52A, the cotton layer, 61A, the water dropper, 62A, the connecting tube, 81A, the fixed pin, 111A, protection casing, 131A, the guide way, 132A, the step.
Detailed Description
The invention will be further described with reference to the following drawings and specific embodiments:
example (b):
referring to fig. 1, the present embodiment provides a method for killing tobacco borer in storage tobacco by feeding and transporting adult bracon hebetor using tobacco borer as host, comprising the following steps:
s1, preparing an artificial feed for raising the larvae of the tobacco meal moth: the artificial feed comprises the following components in parts by weight: 28% of dry corn grit, 42.3% of dry crushed wheat, 7% of yeast powder, 2.5% of cooked soybean meal, 3% of oatmeal, 0.25% of methyl nipagin, 0.25% of sorbic acid, 0.2% of moroxydine hydrochloride tablets, 7% of glycerol, 6.5% of honey and 3% of water; the preparation method comprises the steps of weighing the materials and then stirring;
s2, transferring the 1-3d eclosion adult tobacco meal moth larvae population into an ovipositing box, wherein the environment temperature of the ovipositing box is 28 ℃, the relative humidity is 75% and the photoperiod is 16L; adding 25% of honey water into the spawning box every day, and collecting eggs in the spawning box in a period of 1-3 d;
s3, placing the artificial feed prepared in the S1 into a first feeding box, uniformly scattering the eggs collected in the S2 into the first feeding box, and placing the first feeding box into an environment with the same environmental conditions as the egg laying box; in the feeding process after the first feeding box is arranged in the artificial climate chamber or the artificial climate box, the growth and development of the larvae, the infection of pathogenic bacteria and the eclosion of adults are observed every day, if the larvae are infected with the pathogenic bacteria, the larvae are immediately isolated, and the feed cannot be stirred in the larvae and pupal stage;
s4, transferring a certain number of larvae of the tobacco meal moth larvae from the first rearing box to the second rearing box after the eggs of the tobacco meal moth larvae are hatched into larvae, and introducing paired 1-generation bracon hebetor imagoes into the second rearing box; adding honey water with concentration of 30% into the second feeding box every day; placing the second feeding box in an environment with the same environmental conditions as the spawning box; obtaining 2 generations of bracon hebetor after the 1 generation of bracon hebetor parasitizes and spawns the tobacco pink moth larvae;
s5, after the eggs of the 2-generation bracon hebetor in the S4 are finally eclosized into adult bees through eggs, larvae and pupae, transferring the second feeding box to the environment with the environmental temperature of 4 ℃ until the adult bees are anesthetized at low temperature and cannot move; transferring the adult braconid flies of the male moths from the second feeding box to a transport box for loading;
s6, transporting the transport box to a warehouse where tobacco leaves are stored, and transferring the moth cocoon bees in the transport box into a bee releasing device; and opening the bee placer for releasing the bees when the tobacco pink borer larvae appear on the tobacco bale, and parasitizing and killing the tobacco pink borer on the tobacco leaves by the adult wasps with the moth braconid.
The ratio of the number of the tobacco meal moth larvae to the number of the paired bracon hebetor larvae in the step S2 is 10: 1.
The second feeding box is of a cylindrical box body structure with the bottom surface diameter of 10cm and the height of 8 cm.
The spawning box that uses in step S2 includes bottle 1 and the bottle lid 2 of setting on bottle 1 bleeder vent 3 has been seted up to the up end of bottle lid 2 be equipped with the first gauze 4 that is used for sealing in the bleeder vent 3 the bottle 1 is located the opening of bottleneck below department and has gone into insect population 5 go into insect population 5 department and seal with cotton plug 6 the round hole has been seted up to the bottom of bottle 1 be equipped with the second gauze 7 that is used for sealing in the round hole, the below of bottle 1 is provided with album ovum dish 8. In the embodiment, the first gauze 4, the tampon 6 and the second gauze 7 are all detachable structures with the bottle body 1.
When the oviposition box is used, the tobacco meal moth imagoes are transferred into the oviposition box through the insect inlet 5 and then sealed by the cotton plug 6; dipping honey water with the concentration of 20% on the first gauze 4 to feed tobacco powder borer adults, replacing the first gauze 4 every day, collecting eggs in a period determined according to the specific growth time generally within 1-3d, lightly brushing the eggs stuck on the second gauze 7 by using a writing brush during egg collection, brushing the eggs on an egg collection plate 8, taking down the egg collection plate 8 to collect the eggs, and then putting back the egg collection plate.
The transportation box used in the step S5 includes a square box body 1A, and an installation groove 2A is provided on the upper part of the inner wall of the box body 1A; the top cover 11A is arranged at the top of the box body 1A in a drawing way through the mounting groove 2A; a partition plate 3A is arranged in the middle of the box body 1A; the box body 1A is divided into a plurality of unit cells 14A by the partition boards 3A; a positioning block 4A is arranged on the inner wall of each unit cell 14A; the bee storage tank 5A is detachably arranged in the box body 1A through the positioning block 4A; more than one drip irrigation main pipe 6A is arranged on the lower bottom end surface of the top cover 11A opposite to the box body 1A; the lower bottom of the drip irrigation main pipe 6A is provided with drippers 61A with the positions and the number corresponding to the cells 14A; the dripper 61A is communicated with the drip irrigation main pipe 6A; one end of the drip irrigation main pipe 6A is connected with the honey water container 7A through a connecting pipe 62A; a nutrient liquid pipe 51A communicated with the inside and the outside of the bee storage tank 5A is arranged on the top end surface of the bee storage tank 5A; the adult braconid flies are placed in the bee storage tank 5A.
The bee storage tank 5A is of a cylindrical structure, the upper top surface of the bee storage tank is of an inwards concave conical surface structure, the outer wall of the lower end of the bee storage tank is provided with external threads, and the positioning blocks 4A are of four column block structures which are symmetrically arranged in a cross manner in the cells 14A; and one side of the positioning block 4A connected with the bee storage tank 5A is of a cambered surface structure; an inner screw section matched with the outer screw section is arranged on the arc surface side where the positioning block 4A is connected with the bee storage tank 5A; nutrient solution pipe 51A stretches out one side at storage bee jar 5A top and is the funnel structure, and nutrient solution pipe 51A stretches into and opens on the pipeline in the storage bee jar 5A has the overflow hole, and has wrapped up in cotton layer 52A in the outside of pipeline.
The four walls of the box body 1A are enclosed by three long plates 12A and a short plate 13A with the same structure; the mounting grooves are arranged on the three long plates 12A at positions higher than the short plates 13A, and the lower end face of the top cover 11A is also provided with a protective sleeve 111A; the protective sleeve 111A surrounds the drip irrigation main pipe 6A along the circumferential direction of the drip irrigation main pipe 6A; a through hole is formed at the position of the protective sleeve 111A opposite to the dripper 61A; the upper top of the short plate 13A is provided with a step 132A; the step 132A is provided with its step facing the outside of the case 1A; the top cover 11A is of an L-shaped plate structure, and one end of the L-shaped plate is lapped on the step 132A; the outer end faces of the L-shaped plate and the step 132A are respectively provided with a connecting block 8A; the two connecting blocks 8A are provided with pin holes and fixed by fixing pins 81A.
When the transportation box in the embodiment is used for transporting the bracon hebetor, the movable box body 2A is firstly installed in the outer packaging box along the positioning slot 13A, the bee storage tank 6A containing the adult bracon hebetor pupae is inserted into the movable box body 2A along the positioning block 5A for fixing, and then the upper top opening of the outer packaging box is sealed; the whole external packing box is placed in a transport vehicle, and after the belt packing is finished, the air outlet short of the air conditioning pump 3A is connected with the first air inlet hole 11A through an air pipe; in the transportation process, only the switch of the air cooling pump 3A needs to be opened to enable cold air to continuously cool and ventilate the inside of the movable box body 2A through the holes of the device, so that the survival rate of the bracon hebetor is improved in the transportation process.
Example 2:
meanwhile, the invention also provides a feed for artificially breeding the larvae of the tobacco meal moth;
before preparing the feed, the corn grit and the half-crushed wheat are dried in an oven (60 ℃) for 1-3 hours to obtain the feed, the total weight of the prepared feed is determined, A, B, C three groups of experiments are made for comparison, the weight of each group of components is weighed according to the corresponding proportion (see table 1), the solid is weighed firstly, the liquid is weighed, and after all the materials are weighed, the materials are stirred uniformly;
TABLE 1 tobacco meal moth artificial feed formulation (g)
Figure BDA0002086439280000071
According to the feeding results in step S3, the artificial feed of the present invention was evaluated and analyzed as follows:
(1) the development period is as follows:
the tobacco meal moth eaten by the three feed formulas has insignificant development difference in the egg stage, the larva stage and the pupal stage, particularly has little difference in the egg stage. However, the survival rate difference from eggs to adults is remarkable (P <0.05), the survival rate of the feed A is as high as 75.91%, and the survival rate of the feed C is as low as 69.42% (table 2), but the survival rate of the tobacco pink borer bred by the three feeds is greater than that of the optimal formula C obtained by the high-home and the research of the people and the like.
TABLE 2 influence of the three feed formulations on the survival rate of the tobacco meal moth at the immature stage and from egg to adult
Figure BDA0002086439280000072
Note: values are mean ± sem. The different lower case letters in each column indicate significant differences (P <0.05, Duncan)
(2) Adult life, single-female egg laying amount and sex ratio:
as can be seen from Table 3, the three feed formulations have insignificant effects on the life span, the single female egg laying amount and the male-female ratio of the male-female adults of the tobacco meal moth. The average life of the female and male worms fed by the formula C is 10.00d and 9.00d respectively, which are higher than those of the other two feed formulas, and the egg laying amount of the female is the highest (67.40 grains). The sex ratio of the tobacco powder borers bred by the three feed formulas is close to 1: 1.
TABLE 3 influence of three feed formulations on the service life, ovipositivity and sex ratio of the tobacco meal moth imagoes
Figure BDA0002086439280000073
From the results, a large number of populations can be obtained by feeding the tobacco meal moth with the three feed formulas, but indexes such as a larval stage, a pupal stage, a survival rate and the like and economic cost are comprehensively considered, the feed B is considered to be the optimal formula of the tobacco meal moth, and the probability of the larval infection with viruses can be reduced by adding moroxydine hydrochloride tablets in the invention. Therefore, the formula has low economic cost, the bred insects grow consistently and can obtain a large number of adults, which has important significance for population multiplication. Therefore, the feed B has good development and application prospects for raising the tobacco meal moth.

Claims (6)

1. A method for killing tobacco leaf pink borers in storage by raising and transporting adult wheat moth bracon flies with the tobacco leaf pink borers as hosts is characterized in that: the method comprises the following steps:
s1, preparing an artificial feed for raising the larvae of the tobacco meal moth: the artificial feed comprises the following components in parts by weight: 27-33% of dry corn grit, 36.3-73.3% of dry broken wheat, 5.5-8.5% of yeast powder, 1.2-2.8% of cooked soybean powder, 0-7% of oatmeal, 0.15-0.35% of methyl ni-platinum, 0.10-0.4% of sorbic acid, 0.1-0.3% of moroxydine hydrochloride tablets, 6-8% of glycerol, 5-9% of honey and 2.5-3.5% of water; the preparation method comprises the steps of weighing the materials and then stirring;
s2, transferring the 1-3d eclosion adult tobacco meal moth larvae population into an oviposition box, wherein the environment temperature of the oviposition box is 27-29 ℃, the relative humidity is 70-80%, and the photoperiod is 16L; adding 20-30% of honey water into the spawning box every day, and collecting eggs in the spawning box in a period of 1-3 d;
s3, placing the artificial feed prepared in the S1 into a first feeding box, uniformly scattering the eggs collected in the S2 into the first feeding box, and placing the first feeding box into an environment with the same environmental conditions as the egg laying box;
s4, transferring a certain number of larvae of the tobacco meal moth larvae from the first rearing box to the second rearing box after the eggs of the tobacco meal moth larvae are hatched into larvae, and introducing paired 1-generation bracon hebetor imagoes into the second rearing box; adding 10-70% honey sugar water into the second feeding box every day; placing the second feeding box in an environment with the same environmental conditions as the spawning box; obtaining 2 generations of bracon hebetor after the 1 generation of bracon hebetor parasitizes and spawns the tobacco pink moth larvae; wherein the ratio of the number of the tobacco pink borer larvae to the number of the paired bracon hebetor is 10: 1;
s5, after the eggs, larvae and pupae of the 2-generation braconid flies in the S4 are finally eclosized into adult flies, the second feeding box is transferred to the environment with the temperature of 2-6 ℃ until the adult flies are anesthetized at low temperature and can not move; transferring the adult braconid flies of the male moths from the second feeding box to a transport box for loading;
s6, transporting the transport box to a warehouse where tobacco leaves are stored, and transferring the moth cocoon bees in the transport box into a bee releasing device; and opening the bee placer for releasing the bees when the tobacco pink borer larvae appear on the tobacco bale, and parasitizing and killing the tobacco pink borer on the tobacco leaves by the adult wasps with the moth braconid.
2. The method for killing the tobacco meal moth in the warehouse by feeding and transporting the adult bracon hebetor with the tobacco meal moth as a host according to claim 1, which is characterized in that: the second feeding box is of a cylindrical box body structure with the bottom surface diameter of 10cm and the height of 8 cm.
3. The method for killing the tobacco meal moth in the warehouse by feeding and transporting the adult bracon hebetor with the tobacco meal moth as a host according to claim 1, which is characterized in that: the spawning box that uses in step S2 includes bottle (1) and bottle lid (2) of setting on bottle (1) bleeder vent (3) have been seted up to the up end of bottle lid (2) be equipped with in bleeder vent (3) and be used for the first gauze (4) that seal bottle (1) are located bottleneck below department and open there is insect inlet (5) department seals with cotton plug (6) round hole has been seted up to the bottom of bottle (1) be equipped with in the round hole and be used for the second gauze (7) that seal, the below of bottle (1) is provided with album ovum dish (8).
4. The method for killing the tobacco meal moth in the warehouse by feeding and transporting the adult bracon hebetor with the tobacco meal moth as a host according to claim 1, which is characterized in that: the transportation box used in the step S5 comprises a square box body (1A), wherein the upper part of the inner wall of the box body (1A) is provided with a mounting groove (2A); the top cover (11A) is installed at the top of the box body (1A) in a drawing way through the installation groove (2A); a clapboard (3A) is arranged in the middle of the box body (1A); the box body (1A) is divided into a plurality of cells (14A) by the partition boards (3A); a positioning block (4A) is arranged on the inner wall of each unit cell (14A); the bee storage tank (5A) is detachably arranged in the box body (1A) through the positioning block (4A); more than one drip irrigation main pipe (6A) is arranged on the lower bottom end surface of the top cover (11A) opposite to the box body (1A); the lower bottom of the drip irrigation main pipe (6A) is provided with drippers (61A) the positions and the number of which correspond to the cells (14A); the dripper (61A) is communicated with the drip irrigation main pipe (6A); one end of the drip irrigation main pipe (6A) is connected with the honey water container (7A) through a connecting pipe (62A); a nutrient solution pipe (51A) communicated with the inside and the outside of the bee storage tank (5A) is arranged on the top end surface of the bee storage tank (5A); the adult braconid flies of the moth cocoon are placed in the bee storage tank (5A).
5. The method for killing the tobacco meal moth in the warehouse by feeding and transporting the adult bracon hebetor with the tobacco meal moth as a host according to claim 4, which is characterized in that: the bee storage tank (5A) is of a cylindrical structure, the upper top surface of the bee storage tank is of an inwards concave conical surface structure, the outer wall of the lower end of the bee storage tank is provided with external threads, and the positioning blocks (4A) are of four column block-shaped structures which are symmetrically arranged in a cross manner in the unit cells (14A); and one side of the positioning block (4A) connected with the bee storage tank (5A) is of a cambered surface structure; an inner screw section matched with the outer screw section is arranged on the side of the cambered surface connected with the positioning block (4A) and the bee storage tank (5A); one side of the nutrient solution pipe (51A) extending out of the top of the bee storage tank (5A) is of a funnel structure, an overflow hole is formed in a pipeline of the nutrient solution pipe (51A) extending into the bee storage tank (5A), and a cotton layer (52A) is wrapped on the outer side of the pipeline.
6. The method for killing the tobacco meal moth in the warehouse by feeding and transporting the adult bracon hebetor with the tobacco meal moth as a host according to claim 4, which is characterized in that: the four walls of the box body (1A) are surrounded by three long plates (12A) with the same structure and a short plate (13A); the mounting grooves are arranged on the three long plates (12A) at positions higher than the short plates (13A), and the lower end face of the top cover (11A) is also provided with a protective sleeve (111A); the protective sleeve (111A) surrounds the drip irrigation main pipe (6A) along the circumferential direction of the drip irrigation main pipe (6A); a through hole is formed at the position of the protective sleeve (111A) opposite to the dripper (61A); a step (132A) is arranged at the upper top of the short plate (13A); the step (132A) is arranged facing the outside of the box body (1A); the top cover (11A) is of an L-shaped plate structure, and one end of the L-shaped plate is lapped on the step (132A); the outer end faces of the L-shaped plate and the step (132A) are respectively provided with a connecting block (8A); the two connecting blocks (8A) are provided with pin holes and are fixed through fixing pins (81A).
CN201910489260.2A 2019-06-06 2019-06-06 Method for killing tobacco leaf pink borers in storage by feeding and transporting adult meadowworms with tobacco pink borers as hosts Active CN110235857B (en)

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