CN110123946A - A kind of process using saccharomyces cerevisiae solid state fermentation rhizoma polygonati - Google Patents
A kind of process using saccharomyces cerevisiae solid state fermentation rhizoma polygonati Download PDFInfo
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Abstract
The present invention provides a kind of process using saccharomyces cerevisiae solid state fermentation rhizoma polygonati, comprising the following steps: step 1 takes clean cadmium yellow essence, cuts 1~2mm sheet after steaming 30min~2h, ultraviolet light irradiates 30~40min after drying, obtains fermentation substrate；Step 2 takes saccharomyces cerevisiae strain, culture of crossing on solid medium, by the fluid nutrient medium culture of the single colonie of acquisition；Bacterium solution in step 2 is accessed fluid nutrient medium culture to logarithmic phase latter stage according to the ratio of 3:100 by step 3, obtains brewer's yeast fermented liquid；Fermentation substrate in step 1 and the fermentation liquid in step 3 are carried out solid state fermentation by step 4；After step 5, solid state fermentation, crushed after fermenting mixture is dried.The present invention ferments to rhizoma polygonati using saccharomyces cerevisiae, irritating simultaneously in the raw sealwort for reducing rhizoma polygonati, can generate light durable aroma, lays the foundation for exploitation King solomonseal rhizome tea and drink.
Description
Technical field
The present invention relates to a kind of processes of rhizoma polygonati that ferments, and in particular to a kind of yellow using saccharomyces cerevisiae solid state fermentation
The process of essence.
Background technique
Rhizoma polygonati is polygonatum kingianurn Polygonatum kingianum coll.et Hemsl., rhizoma polygonati Polygonatum
The dry rhizome of sibirifum Red. and polygonatum cyrtonema Polygonatum cyrtonema Hua.Rhizoma polygonati is sweet in flavor, being capable of tonifying Qi
Yin-nourishing, invigorating the spleen, moistening lung, kidney-nourishing are one of Chinese medicine common drugs.Rhizoma polygonati main component have polysaccharide, saponin(e, alkaloid, lignanoid,
Flavones and volatile oil etc., polysaccharide account for 4.47%~21.34% or so, and wherein polysaccharide and saponin(e are main pharmacodynamics ingredients.Modern times grind
Study carefully and shows that rhizoma polygonati has hypoglycemic, Adjust-blood lipid, adjusting immunity, antitumor, anti-aging, antibacterial and improving studing ability etc.
Pharmacological action.
Raw sealwort has numb taste, and when health product is taken, dispute is numb, stimulates throat, and be easy to cause diarrhea.Ancients are for Huang
The method that the processing of essence mostly uses " nine, which steam nine, shines ", this traditional handicraft are also used till today always, but long the time required to this method,
The process is more complicated, time-consuming.Therefore, from long-range economic benefit, the processing technology of rhizoma polygonati is badly in need of injecting new skill
Art, come solve the problems, such as there is currently.
Saccharomyces cerevisiae is common fermented yeast in Beer Brewage, in addition to for beer brewing, is also commonly used for fermentation face
Packet.Saccharomyces cerevisiae thallus vitamin, protein content are high, can eat, medicinal and fodder yeast, can also be from wherein extracting
Cromoci, nucleic acid, glutathione, thromboplastinum, coacetylase and atriphos etc..Brewer's yeast is practically free of fat, starch
And sugar, contain excellent protein, complete B family vitamin, a variety of biological state minerals and high-quality dietary fiber.Due to beer
The characteristic of saccharomycete is deeply recognized, and brewer's yeast is now widely used in the guarantor such as weight reducing, diabetes, fatty liver, Vitamin B deficiency
Strong field.If the zymotechnique of rhizoma polygonati can be successfully applied to, it is not only a very big innovation, and certainly will be to rhizoma polygonati
The further exploitation of medicinal material is played the role of vital.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of technique using saccharomyces cerevisiae solid state fermentation rhizoma polygonati
Method, this method can significantly reduce the irritation of rhizoma polygonati, shorten rhizoma polygonati process time, improve the quality and curative effect of rhizoma polygonati medicinal material.
The technical solution of the present invention is as follows:
A kind of process using saccharomyces cerevisiae solid state fermentation rhizoma polygonati, comprising the following steps:
Step 1 takes clean cadmium yellow essence, cuts 1~2mm sheet after steaming 30min~2h, and ultraviolet light irradiation 30 after drying~
40min obtains fermentation substrate；
Step 2 takes saccharomyces cerevisiae strain, and culture of crossing on solid medium trains the single colonie of acquisition with liquid
Support base culture；
Bacterium solution in step 2 is accessed fluid nutrient medium culture to logarithmic phase latter stage according to the ratio of 3:100 by step 3, is obtained
To brewer's yeast fermented liquid；
Fermentation substrate in step 1 and the fermentation liquid in step 3 are carried out solid state fermentation by step 4；
After step 5, solid state fermentation, crushed after fermenting mixture is dried.
Further, cross on solid medium in the step 2 culture time be 45~60h.
Further, the composition of the solid medium are as follows: brewer's wort dry powder 0.131g/mL, agar 0.02g/mL.
Further, by the single colonie of the acquisition condition of fluid nutrient medium culture in the step 2 are as follows: 37 DEG C of temperature,
Revolving speed 180rpm, 7~10h of incubation time.
Further, the condition of culture in the step 3 are as follows: 37 DEG C of temperature, revolving speed 120rpm.
Further, the composition of the fluid nutrient medium are as follows: brewer's wort dry powder 0.131g/mL.
Further, in the step 4 solid state fermentation condition are as follows: bacterium material is than 5~25%, 37 DEG C of fermentation temperature, the time
40~60h.
Further, the condition of solid state fermentation is preferred in the step 4 are as follows: and bacterium material is than 15%, and 37 DEG C of fermentation temperature, the time
48h。
Further, the condition of the drying are as follows: 55~65 DEG C of temperature, time 20min~6h.
The beneficial effects of the present invention are: the present invention ferments to rhizoma polygonati using saccharomyces cerevisiae, in the life for reducing rhizoma polygonati
Rhizoma polygonati is irritating simultaneously, can generate light durable aroma, lays the foundation for exploitation King solomonseal rhizome tea and drink.Also, pharmacology is real
Verify bright, the rhizoma polygonati after being fermented using saccharomyces cerevisiae can significantly increase the spleen of mouse and the organ index of thymus gland, and can drop
The content of low MDA increases the content of hepatic glycogen and muscle glycogen, moreover it is possible to extend the swimming time of mouse.This illustrates that rhizoma polygonati is sent out
Better antifatigue, anti-oxidant and immunological enhancement can be played after ferment.
Specific embodiment
The bacterial strain (Sac-charomyces cerevisiae) of saccharomyces cerevisiae used in the embodiment of the present invention is purchased from Shanghai
Lu Wei Science and Technology Ltd., bacterial strain deposit number are ATCC9763.
In the description of the present invention, it should be noted that the person that is not specified actual conditions in embodiment, according to normal conditions or
The condition that manufacturer suggests carries out.Reagents or instruments used without specified manufacturer is that can be obtained by commercially available purchase
Conventional products.
The present invention is described in further details below with reference to specific embodiment, it is described be explanation of the invention without
It is to limit.
Embodiment 1
The present embodiment provides a kind of processes using saccharomyces cerevisiae solid state fermentation rhizoma polygonati, comprising the following steps:
Step 1 takes clean cadmium yellow essence, cuts 2mm sheet after steaming 1h, with ultraviolet light irradiation 30min after 60 DEG C of baking 2h, obtains
Fermentation substrate；
Step 2 takes saccharomyces cerevisiae strain, the scribing line culture 45h on solid medium, by the single colonie liquid of acquisition
Culture is based on 37 DEG C, revolving speed 180rpm, culture 7h；
Bacterium solution in step 2 is accessed fluid nutrient medium according to the ratio of 3:100 by step 3, in 37 DEG C, the item of 120rpm
Culture obtains brewer's yeast fermented liquid to logarithmic phase latter stage under part；
Fermentation substrate in step 1 and the fermentation liquid in step 3 are carried out solid state fermentation, solid state fermentation conditions by step 4
Are as follows: bacterium material is than 5%, and 37 DEG C of fermentation temperature, time 40h；
After step 5, solid state fermentation, fermenting mixture is crushed after 60 DEG C of baking 2h.
The composition of the solid medium are as follows: brewer's wort dry powder 0.131g/mL, agar 0.02g/mL.
The composition of the fluid nutrient medium are as follows: brewer's wort dry powder 0.131g/mL.
Embodiment 2
The present embodiment provides a kind of processes using saccharomyces cerevisiae solid state fermentation rhizoma polygonati, comprising the following steps:
Step 1 takes clean cadmium yellow essence, cuts 2mm sheet after steaming 1h, with ultraviolet light irradiation 30min after 60 DEG C of baking 1h, obtains
Fermentation substrate；
Step 2 takes saccharomyces cerevisiae strain, the scribing line culture 50h on solid medium, by the single colonie liquid of acquisition
Culture is based on 37 DEG C, revolving speed 180rpm, culture 7h；
Bacterium solution in step 2 is accessed fluid nutrient medium according to the ratio of 3:100 by step 3, in 37 DEG C, the item of 120rpm
Culture obtains brewer's yeast fermented liquid to logarithmic phase latter stage under part；
Fermentation substrate in step 1 and the fermentation liquid in step 3 are carried out solid state fermentation, solid state fermentation conditions by step 4
Are as follows: bacterium material is than 15%, and 37 DEG C of fermentation temperature, time 48h；
After step 5, solid state fermentation, fermenting mixture is crushed after 60 DEG C of baking 2h.
The composition of the solid medium are as follows: brewer's wort dry powder 0.131g/mL, agar 0.02g/mL.
The composition of the fluid nutrient medium are as follows: brewer's wort dry powder 0.131g/mL.
Embodiment 3
The present embodiment provides a kind of processes using saccharomyces cerevisiae solid state fermentation rhizoma polygonati, comprising the following steps:
Step 1 takes clean cadmium yellow essence, cuts 2mm sheet after steaming 1h, with ultraviolet light irradiation 30min after 60 DEG C of baking 2h, obtains
Fermentation substrate；
Step 2 takes saccharomyces cerevisiae strain, the scribing line culture 60h on solid medium, by the single colonie liquid of acquisition
Culture is based on 37 DEG C, revolving speed 180rpm, culture 8h；
Bacterium solution in step 2 is accessed fluid nutrient medium according to the ratio of 3:100 by step 3, in 37 DEG C, the item of 120rpm
Culture obtains brewer's yeast fermented liquid to logarithmic phase latter stage under part；
Fermentation substrate in step 1 and the fermentation liquid in step 3 are carried out solid state fermentation, solid state fermentation conditions by step 4
Are as follows: bacterium material is than 25%, and 37 DEG C of fermentation temperature, time 55h；
After step 5, solid state fermentation, fermenting mixture is crushed after 60 DEG C of baking 1h.
The composition of the solid medium are as follows: brewer's wort dry powder 0.131g/mL, agar 0.02g/mL.
The composition of the fluid nutrient medium are as follows: brewer's wort dry powder 0.131g/mL.
Comparative example 1
The present embodiment provides a kind of processes of rhizoma polygonati that ferments, according to the process of tradition " nine, which steam nine, shines ", specifically
Operation are as follows: clean cadmium yellow essence is removed into fibrous root, be steamed in pot 6h, and taking-up is dried, and it shines one and steams again, 3~4 times repeatedly, until interior
There is black in the heart, when turbid profit until, taking-up is dried or is dried with small fire, is crushed.
Embodiment 4
The rhizoma polygonati powder and raw sealwort prepare to Examples 1 to 3, comparative example 1 carries out quality testing:
1) properties and characteristics compare
Example 1~3, comparative example 1 and raw sealwort are compared rhizoma polygonati quality from color, quality, gas, taste etc.,
The results are shown in Table 1.
1 rhizoma polygonati of table fermentation front and back character compares
2) measurement of Siberian solomonseal rhizome polysaccharide content
A, the preparation of standard curve
Using DEXTROSE ANHYDROUS as reference substance, reference substance solution is prepared, takes solution 0.2mL in cleaned glass test tube.Using
Phend-sulphuric acid adds 5% phenol solution 0.2mL, shakes up, be rapidly added concentrated sulfuric acid 1mL, shakes up postposition and react at room temperature
40min.The solution 0.2mL after answering is negated in 96 orifice plates, with extinction of the microplate reader measurement glucose solution under 490nm wavelength
Degree draws standard curve.
B, polysaccharide determination
Rhizoma polygonati powder and cadmium yellow essence sample prepared by Example 1~3, comparative example 1, takes after 60 DEG C of drying to constant weights
0.25g, it is accurately weighed, it sets in round-bottomed flask, adds 80% ethyl alcohol 150ml, set and be heated to reflux in water-bath 1 hour, filtered while hot, it is residual
Slag is washed 3 times with 80% hot ethanol, each 10ml.Residue and filter paper are set in flask, adds water 150ml, sets in boiling water bath and heat
Reflux 1 hour, filters while hot, and residue and flask are washed 4 times, each 10ml with hot water, and merging filtrate and washing lotion are let cool, transfer
Into 250ml measuring bottle, scale is added water to, is shaken up, test solution is obtained.The method measurement of absorbance is surveyed for examination with reference substance
The absorbance of product solution obtains the concentration (mg/mL) of DEXTROSE ANHYDROUS in sample according to standard curve, finally determines Huang after fermentation
The polyoses content of essence.Measurement result is as shown in table 2, and formula is such as shown in [1]:
2 Siberian solomonseal rhizome polysaccharide content of table
3) measurement of rhizoma polygonati extract content
Example 1~3 and comparative example 1 and cadmium yellow essence sample, each sample about 2g.It is accurately weighed, set the cone of 100ml
In shape bottle, precision plus ethyl alcohol 50ml, close plug, weighed weight.After standing 1 hour, reflux condensing tube is connected, is heated to boiling, and
It is kept for slightly boiled 1 hour.After letting cool, conical flask, close plug, then weighed weight are removed, the weight of less loss is supplied with ethyl alcohol, is shaken up, used
Dry filter filtration.Precision measures filtrate 25mL, sets and has dried into the evaporating dish of constant weight, after being evaporated in water-bath.In 105 DEG C
It is 3 hours dry, set 30 minutes cooling in drier, rapid accurately weighed weight.Water-soluble leaching in test sample is calculated with dry product
The content (%) of object out.Measurement result is as shown in table 3, shown in calculation formula such as formula [2]:
Content (%)=[m of water-soluble extractives2-m0] * V/25m1*100%, [2]
In formula [2], m0For the dry evaporating dish weight to constant weight, m1For test sample quality, m2For the dry evaporation to constant weight
The total weight of ware and test sample, V are the volume of filtrate)
3 rhizoma polygonati extract content of table
4) assay of total saposins
A, the preparation of reference substance solution
Precision weighs Dioscin reference substance 2.0mg, is placed in 5mL volumetric flask, adds methanol to dissolve and is diluted to scale, shakes
It is even to get every 1mL Dioscin containing 0.4mg reference substance solution.
B, prepared by test solution
Rhizoma polygonati powder and cadmium yellow essence sample prepared by Example 1~3, comparative example 1, accurate title after 60 DEG C of drying to constant weights
0.5g is taken, ethyl alcohol 15mL, 60 DEG C of ultrasonic extraction 50min are added, is filtered, 80% ethyl alcohol of 15mL, 60 DEG C of ultrasonic extractions are added in the dregs of a decoction
45min, filtering merge filtrate twice.Constant volume is into 50mL volumetric flask up to test solution.
C, the determination of Detection wavelength
Absorption comparison liquid 0.5mL, test liquid 0.1mL volatilize methanol in tool plug test tube, water-bath respectively, and precision, which is added, newly matches
5% (w/v) vanillic aldehyde glacial acetic acid solution of system: perchloric acid (2:8, v/v) mixed liquor 1.0mL shakes up, sets 60 DEG C of water-bath 25min,
Cooling in cold water is set rapidly, it is rear that 5mL glacial acetic acid is added, it shakes up, with ultraviolet-visible spectrophotometer within the scope of 400-700nm
Scanning, determines a length of measurement wavelength of maximum absorption wave, is 550nm.
D, prepared by standard curve
Accurate 0,0.05,0.10,0.20,0.30,0.40,0.50,0.60mL reference substance solution of drawing is in tool plug examination respectively
Pipe, water-bath volatilize methanol, and 5% (w/v) vanillic aldehyde glacial acetic acid solution newly configured: perchloric acid (2:8) mixed liquor is added in precision
1.0mL shakes up, and sets 60 DEG C of water-bath 25min, sets cooling in cold water rapidly, rear that 5mL glacial acetic acid is added, and shakes up.It is with reagent blank
Control measures absorbance value A in surveying given wavelength.Using the A value measured at 550nm as ordinate, it is with Dioscin content X
Abscissa draws standard curve, obtains equation of linear regression.
E, measuring method
Accurate measuring test solution 0.1mL volatilizes methanol in tool plug test tube, water-bath, develops the color by C, middle method, with reagent
Blank is control, measures A value in surveying given wavelength, and calculate content according to standard curve, takes 3 average values as measurement knot
Fruit.The results are shown in Table 4.
4 saponins of rhizoma polygonati content of table
Pass through the data of table 1~4, it can be seen that the content of rhizoma polygonati extract, polysaccharide and total saposins after everfermentation is than passing
System " nine steam nine systems " gained sample and raw sealwort increased, wherein with 2 content highest of embodiment.As it can be seen that using brewer's yeast
After bacterium solid state fermentation rhizoma polygonati, its numb taste and throat hurt feeling are not removed only, fragrance is increased, also makes active constituent content obvious
Increase.
Embodiment 5
Pharmacological testing
One, the preparation of tested material drug
(1) preparation of fermentation rhizoma polygonati extracting solution
Rhizoma polygonati prepared by Example 1~3 and comparative example 1, adds 10 times of amount distilled water, immersion 30min, with being cooked by slow fire
0.5h, the distilled water of second plus 8 times amount are cooked by slow fire 0.5h, merge 2 fried liquid, stand filter and remove residue, are concentrated as crude drug
The rhizoma polygonati extracting solution of 0.60g/mL is measured, it is spare.
(2) preparation of raw sealwort and Ginseng extract
Raw sealwort and ginseng are taken, respectively plus 10 times of amount distilled water, immersion 30min add 8 with 0.5h is cooked by slow fire for the second time
The distilled water of amount is cooked by slow fire 0.5h again, merges 2 fried liquid, stands filter and remove residue, is concentrated as the life of crude drug amount 0.60g/mL
Rhizoma polygonati extracting solution and Ginseng extract.
Two, experimental animal grouping and processing
Cleaning grade male mice in kunming 70.7 groups are randomly divided into, every group 10, respectively Examples 1 to 3 group compare
1 group of example, raw sealwort control group, ginseng control group and blank group.Each administration group liquor strength is crude drug amount 0.60g/mL.More than
7 groups, by stomach-filling volume 0.1mL/10g gastric infusion, negative control group (blank group) is filled with the physiological saline of same volume.Daily
Sooner or later it after each stomach-filling 1 time, continuous gavage 15d, last dose 30min, carries out swimming with a load attached to the body power and exhausts experiment.
Three, mouse power exhausts swimming test method
According to the administration mode in step 2: daily sooner or later it is each stomach-filling 1 time, continuous gavage 15d, each perfusion according to
0.1mL/10g, drug concentration is it is known that daily administration amount can be instructed, and according to mouse weight difference, dosage can be poor
It is different.It is negative with Oxford cup (the 5% of mouse weight) in mouse tail root, it is put into water temperature (25.0 ± 0.5) DEG C, the swimming of depth of water 40cm
In case, with stopwatch record from swim time for being exhausted to power.The judgment criteria that power exhausts is that 5s is still unable to emersion after mouse is sunk
The water surface.
Four, Content of MDA measures
Mouse carry out swimming power exhaust experiment after, eyeball blood sampling, be put into 4 DEG C of refrigerators and save.Blood is taken to be centrifuged at 4 DEG C,
Revolving speed is 3500r/min, takes supernatant liquor, detects Content of MDA by kit measurement method.
Five, the measurement of liver glycogen and Body development
Swimming terminates, and puts to death mouse immediately, takes out liver and muscle, is put into -80 DEG C of refrigerators and saves.It is tested and is tried with glycogen
Agent box is measured.
Six, thymus index, index and spleen index
Mouse is put to death, takes out thymus gland and spleen immediately, is cleaned, is blotted with physiological saline, is weighed, with organ wet weight (mg/
G) Thymus and Spleen index is indicated.
Seven, result counts
It is carried out using statistical method according to analysis.Using SPSS20 statistical software, one-way analysis of variance, metering money are carried out
Material is with average valueIt indicates.It is that difference is statistically significant with P < 0.05, P < 0.01 is the great statistical significance of difference.
1) power exhausts swimming test result
As shown in 5 data of table, 6 administration groups are compared with physiological saline group, can significantly improve mouse swimming time,
Wherein ginseng control group and 2 groups of embodiment have extremely significant difference, 2 groups of time longests of embodiment, it is seen that different rhizoma polygonati administration groups and
Ginseng control group all has antifatigue effect, wherein most strong with 2 groups of effects of embodiment.It is compared with ginseng control group, physiology salt
The mouse swimming time of water group, raw sealwort control group and comparative example group significantly reduces, and 1,3 group of embodiment, time drop
It is low, but no difference of science of statistics, 2 groups of times of embodiment are higher than ginseng control group, but no difference of science of statistics.After visible solid state fermentation
The antifatigue significant effect of rhizoma polygonati increases, and 2 groups of embodiment suitable with ginseng.
Influence (n=10) of the 5 rhizoma polygonati extracting solution of table to mouse swimming time
Note: " * ", which is represented, has compared conspicuousness with physiological saline group, and " * * " is represented to have compared with physiological saline group and extremely be shown
Work property；
" △ ", which is represented, has compareed conspicuousness with ginseng group, and " △ △ " representative has compareed extremely significant property with ginseng group.
2) glycogen measures
As shown in 6 data of table, 6 administration groups are compared with physiological saline group, can dramatically increase hepatic glycogen and muscle glycogen
Content, wherein ginseng control group and 2 groups of embodiment have extremely significant difference, 2 groups of hepatic glycogen of embodiment and muscle glycogen content highest, can
See that different rhizoma polygonati administration groups and ginseng control group all have antifatigue effect, wherein most strong with 2 groups of effects of embodiment.And ginseng
Control group is compared, and physiological saline group, the hepatic glycogen of raw sealwort control group and comparative example group and muscle glycogen content significantly reduce, real
Though applying 1,3 group of example has reduction, no difference of science of statistics, the content that 2 groups of embodiment is higher than ginseng control group, but poor without statistics
It is different.The antifatigue significant effect of rhizoma polygonati after visible solid state fermentation increases, and wherein the rhizoma polygonati and ginseng of embodiment 2 are suitable.
Influence of the 6 rhizoma polygonati extracting solution of table to sports fatigue Glycogen
Note: " * ", which is represented, has compared conspicuousness with physiological saline group, and " * * " is represented to have compared with physiological saline group and extremely be shown
Work property；
" △ ", which is represented, has compareed conspicuousness with ginseng group, and " △ △ " representative has compareed extremely significant property with ginseng group.
3) serum MDA measures
As shown in 7 data of table, 6 administration groups are compared with physiological saline group, can significantly reduce the content of MDA, wherein people
Joining control group and 2 groups of embodiment has extremely significant difference, and 2 groups of MDA contents of embodiment are minimum, it is seen that different rhizoma polygonati administration groups and ginseng
Control group all has oxidation resistant effect, wherein most strong with 2 groups of effects of embodiment.It is compared with ginseng control group, removes physiological saline
Group and raw sealwort control group MDA content are significantly higher than outside control group, the not statistically significant difference of remaining each group, embodiment 2
The MDA content of group is slightly below ginseng control group, and the content of each processing group of rhizoma polygonati is below raw sealwort control group, it is seen that rhizoma polygonati passes through
After processing, its antioxidant effect can be improved, wherein the effect of embodiment 2 is best.
Influence of the 7 rhizoma polygonati extracting solution of table to sports fatigue mice serum MDA
Note: " * ", which is represented, has compared conspicuousness with physiological saline group, and " * * " is represented to have compared with physiological saline group and extremely be shown
Work property；
" △ ", which is represented, has compareed conspicuousness with ginseng group, and " △ △ " representative has compareed extremely significant property with ginseng group.
4) organ index measures
As shown in 8 data of table, rhizoma polygonati processing group is compared with physiological saline group, can dramatically increase Thymus and spleen index,
Wherein ginseng control group and 2 groups of embodiment have extremely significant difference, 2 groups of index and spleen index highests of embodiment, raw sealwort control group and life
Salt water group is managed without significant difference, it is seen that rhizoma polygonati administration group and ginseng control group after difference processing all have the work of Immune-enhancing effect
With.It is compared with ginseng control group, except physiological saline group and raw sealwort control group index and spleen index and thymus index are substantially less than and compare
Group is outer, the not statistically significant difference of remaining each group, and the index and spleen index and thymus index that 2 groups of embodiment are compareed higher than ginseng
Group, it is seen that rhizoma polygonati can improve its immunological enhancement, wherein the effect of embodiment 2 is best after processing.
Influence of the 8 rhizoma polygonati extracting solution of table to sports fatigue mice organs index
Note: " * ", which is represented, has compared conspicuousness with physiological saline group, and " * * " is represented to have compared with physiological saline group and extremely be shown
Work property；
" △ ", which is represented, has compareed conspicuousness with ginseng group, and " △ △ " representative has compareed extremely significant property with ginseng group.
To sum up, its effect antifatigue, anti-oxidant and that enhancing is immune, saccharomyces cerevisiae can be improved in rhizoma polygonati after processing
Solid state fermentation rhizoma polygonati becomes apparent from than the reinforcing effect of traditional diamond-making technique, wherein best with 2 groups of effects of embodiment.It can be seen that the present invention
Novel processing method, can reach enhancing rhizoma polygonati curative effect practical application effect.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, modifies, replacement and variant.
Claims (9)
1. a kind of process using saccharomyces cerevisiae solid state fermentation rhizoma polygonati, which comprises the following steps:
Step 1 takes clean cadmium yellow essence, cuts 1~2mm sheet after steaming 30min~2h, ultraviolet light irradiates 30~40min after drying, obtains
To fermentation substrate；
Step 2 takes saccharomyces cerevisiae strain, culture of crossing on solid medium, by the single colonie fluid nutrient medium of acquisition
Culture；
Bacterium solution in step 2 is accessed fluid nutrient medium culture to logarithmic phase latter stage according to the ratio of 3:100 by step 3, obtains beer
Brewer yeast fermented liquid；
Fermentation substrate in step 1 and the fermentation liquid in step 3 are carried out solid state fermentation by step 4；
After step 5, solid state fermentation, crushed after fermenting mixture is dried.
2. a kind of process using saccharomyces cerevisiae solid state fermentation rhizoma polygonati according to claim 1, which is characterized in that
Cross on solid medium in the step 2 culture time be 45~60h.
3. a kind of process using saccharomyces cerevisiae solid state fermentation rhizoma polygonati according to claim 1 or 2, feature exist
In the composition of the solid medium are as follows: brewer's wort dry powder 0.131g/mL, agar 0.02g/mL.
4. a kind of process using saccharomyces cerevisiae solid state fermentation rhizoma polygonati according to claim 1, which is characterized in that
By the single colonie of the acquisition condition of fluid nutrient medium culture in the step 2 are as follows: 37 DEG C of temperature, revolving speed 180rpm, when culture
Between 7~10h.
5. a kind of process using saccharomyces cerevisiae solid state fermentation rhizoma polygonati according to claim 1, which is characterized in that
Condition of culture in the step 3 are as follows: 37 DEG C of temperature, revolving speed 120rpm.
6. according to claim 1, a kind of using saccharomyces cerevisiae solid state fermentation rhizoma polygonati described in 4,5 any one claims
Process, which is characterized in that the composition of the fluid nutrient medium are as follows: brewer's wort dry powder 0.131g/mL.
7. a kind of process using saccharomyces cerevisiae solid state fermentation rhizoma polygonati according to claim 1, which is characterized in that
The condition of solid state fermentation in the step 4 are as follows: bacterium material is than 5~25%, and 37 DEG C of fermentation temperature, 40~60h of time.
8. a kind of process using saccharomyces cerevisiae solid state fermentation rhizoma polygonati according to claim 7, which is characterized in that
The condition of solid state fermentation is preferred in the step 4 are as follows: and bacterium material is than 15%, and 37 DEG C of fermentation temperature, time 48h.
9. a kind of process using saccharomyces cerevisiae solid state fermentation rhizoma polygonati according to claim 1, which is characterized in that
The condition of the drying are as follows: 55~65 DEG C of temperature, time 20min~6h.
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