CN110075937B

CN110075937B - Pipette gun head

Info

Publication number: CN110075937B
Application number: CN201810078895.9A
Authority: CN
Inventors: 郭敏; 王海鹏; 于雪
Original assignee: Kangma Healthcode Shanghai Biotech Co Ltd
Current assignee: Kangma Healthcode Shanghai Biotech Co Ltd
Priority date: 2018-01-26
Filing date: 2018-01-26
Publication date: 2024-09-20
Anticipated expiration: 2038-01-26
Also published as: CN110075937A

Abstract

The invention discloses a pipette tip, which comprises a tip body, wherein the tip body is integrated or integrally formed, the tip body is tubular, the tip body comprises a suction nozzle area, a liquid storage area, a transition area and a pipette action area from bottom to top, and the inner cavity of the tubular tip body is of a tapered structure from top to bottom; the height of the suction nozzle area is 7-10 mm, the apex angle is alpha, wherein alpha is more than or equal to 10 degrees and less than or equal to 45 degrees, and the inner diameter is 0.4-0.9 mm; the height of the liquid storage area is 30-35 mm, the apex angle is beta, wherein beta is more than or equal to 10 degrees and less than or equal to 45 degrees, and the inner diameter is 0.8-1.8 mm; the height of the transition zone is 10-11 mm, and the inner diameter is 1.8-5.4 mm; the height of the action area of the pipette is 5.4-5.8 mm, and the inner diameter is 5.3-6.5 mm. The pipette gun head provided by the invention has the advantages of simple structure, moderate measuring range, high structural strength and high pipetting accuracy, and is suitable for the widely existing experiments of medium and small volumes and high flux.

Description

Pipette gun head

Technical Field

The invention relates to the field of biological experiment equipment, in particular to a small-range and high-precision pipette gun head suitable for biochemical reactions with high flux, small volume and high precision.

Background

With the rapid development of scientific and technical research, more and more high-throughput experiments are widely applied in the fields of biological scientific research, drug development, analytical chemistry, clinical diagnosis and the like. Manual operation has failed to meet the high efficiency, accuracy and safety requirements of high throughput sample processing, automated operation of part or all of the experimental procedures has become a necessary trend, and more liquid workstations have been developed and used. In the fields of life science research, drug research and development, etc., along with the improvement of sensitivity of a used detection instrument and the limitation of factors such as difficulty in sample preparation, etc., small-volume reaction systems and components are increasingly applied, such as microliter (mul) level reaction systems and small-component establishment below microliter level. The small volume reaction system means that higher pipetting accuracy requirements are imposed on the high throughput liquid work stations used, and the design and quality of the pipette tips used are critical for improving pipetting accuracy. The current range of the pipette tips used in many liquid workstations is larger, the precision is lower, such as the common 100 mu L gun tip, and the pipetting precision is 2 mu L; a 50 mu L gun head, and the pipetting accuracy is 1 mu L; or the precision is proper, but the measuring range is smaller, such as a 10 mu L gun head, and the pipetting precision is 0.2 mu L; such pipette tips are difficult to adapt to the current high throughput, medium and small volume and high precision biochemical reaction requirements, and therefore there is an urgent need in the art to develop a pipette tip with a medium range, but high pipetting accuracy that is compatible with currently used liquid workstations.

Disclosure of Invention

The invention aims to provide a pipette tip, which has the advantages of simple structure, moderate measuring range, high structural strength and high pipetting accuracy, and is suitable for the widely existing experiments with medium and small volumes and high flux.

The invention provides a pipette tip, which comprises a tip body, wherein the tip body is integrated or integrally formed, the tip body is tubular, the tip body comprises a suction nozzle area, a liquid storage area, a transition area and a pipette action area from bottom to top, and the inner cavity of the tubular tip body is of a tapered structure from top to bottom; the height of the suction nozzle area is 7-10 mm, the vertex angle is alpha, wherein alpha is more than or equal to 1 degree and less than or equal to 30 degrees, and the inner diameter is 0.4-0.9 mm; the height of the liquid storage area is 30-35 mm, the apex angle is beta, wherein beta is more than or equal to 1 degree and less than or equal to 30 degrees, and the inner diameter is 0.8-1.8 mm; the height of the transition zone is 10-11 mm, and the inner diameter is 1.8-5.4 mm; the height of the action area of the liquid transfer device is 5.4-5.8 mm, and the inner diameter is 5.3-6.5 mm.

It should be noted that, because the inner cavity of the pipette tip of the invention is in a tapered structure from top to bottom, the inner cavities of different sections of the pipette tip form a truncated cone-shaped space, and the cone apex angle of the truncated cone-shaped space corresponding to the cone is the "apex angle" of the invention.

In another preferred embodiment, the suction nozzle area is in communication with the liquid storage area, the liquid storage area is in communication with the transition area, and the transition area is in communication with the pipette action area.

In another preferred example, α=β.

In another preferred embodiment, the transition zone has a plurality of first protrusions uniformly disposed on an outer circumference thereof.

In another preferred embodiment, the number of the first protrusions is 4-12.

In another preferred embodiment, the transition zone comprises a first transition zone and a second transition zone, wherein the first transition zone is fixedly connected with the liquid storage zone, and the second transition zone is fixedly connected with the action zone of the liquid transfer device.

In another preferred embodiment, the inner diameter of the first transition zone is 1/3-2/3 of the inner diameter of the second transition zone.

In another preferred embodiment, the inner diameter of the first transition zone is 2/5 of the inner diameter of the second transition zone.

In a further preferred embodiment, an intermediate transition section is provided between the first transition region and the second transition region.

In another preferred embodiment, the first plurality of protrusions are uniformly disposed on the outer circumference of the intermediate transition section.

In another preferred embodiment, a plurality of second protrusions are uniformly provided on the outer periphery of the action area of the pipette.

In another preferred embodiment, the number of the second protrusions is 4-12.

In another preferred embodiment, the pipette action zone comprises a first action zone and a second action zone, wherein the first action zone is fixedly connected with the transition zone, and the second action zone is fixedly connected with the first action zone.

In another preferred embodiment, the inner diameter of the first active region is 0.5-1.5 mm greater than the inner diameter of the second active region.

In another preferred embodiment, the plurality of second protrusions are uniformly disposed on the outer circumference of the second active region.

In another preferred embodiment, the inner cavity of the active area of the pipette is detachably adapted to the existing conventional pipettes.

In another preferred embodiment, the range of the pipette tip is 20-40 mu L; preferably 30 μl.

In another preferred example, the pipetting accuracy of the pipette tip is 0.3-0.8 mu L; preferably 0.5 μl.

In another preferred embodiment, the gun head is a plastic gun head.

In another preferred embodiment, the gun head is a polypropylene gun head.

In another preferred embodiment, scale marks are arranged on the outer surface of the gun head, so that an operator can conveniently read the approximate volume of the liquid to be measured sucked by the gun head.

In another preferred embodiment, the suction nozzle region, the liquid storage region, the transition region and the pipette action region are coaxially arranged.

The main advantages of the invention include:

(a) The range of the pipette gun head is small, and the pipetting accuracy is high;

(b) Is particularly suitable for a high-flux and small-volume reaction system;

(c) The structure is simple, and the appearance is exquisite;

(d) The action area of the liquid transfer device can be compatible with the liquid transfer device of the currently mainstream high-flux liquid work station, a new liquid work station does not need to be redesigned and built, the cost is reduced, and the liquid transfer device is easy to popularize.

It is understood that within the scope of the present invention, the above-described technical features of the present invention and technical features specifically described below (e.g., in the examples) may be combined with each other to constitute new or preferred technical solutions. And are limited to a space, and are not described in detail herein.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a cross-sectional view of a pipette tip in one example of the invention.

Fig. 2 is a front view of a pipette tip in one example of the invention.

Fig. 3 is a bottom view of a pipette tip in one example of the invention.

Fig. 4 is a perspective view of a pipette tip in one example of the invention.

In the drawings, each is indicated as follows:

1-a suction nozzle area;

2-a liquid storage area;

A 3-transition zone;

4-a pipette action zone;

31-a first transition zone;

32-a second transition zone;

33-a first bump;

41-a first region of action;

42-a second region of action;

43-second bump.

Detailed Description

The present inventors have developed a pipette tip for the first time through extensive and intensive studies, and compared with the prior art, the pipette tip of the present invention is particularly suitable for experiments with medium and small volumes and high throughput, and has the advantages of simple structure, high structural strength, moderate range, high pipetting accuracy, and the pipetting accuracy of 0.5 μl, taking the present tip with a range of 30 μl as an example, and completed the present invention on this basis.

The invention will be further illustrated with reference to specific examples. It is to be understood that these examples are illustrative of the present invention and are not intended to limit the scope of the present invention. Furthermore, the drawings are schematic representations, and thus the apparatus and device of the present invention are not limited by the dimensions or proportions of the schematic representations.

It should be noted that in the claims and the description of this patent, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.

Examples

The invention provides a pipette tip, which is a pipette tip with a specific structure.

The pipette tip of this embodiment is shown in FIGS. 1-4. The pipette tip of the embodiment comprises a tip body, wherein the tip body comprises a suction nozzle area (1), a liquid storage area (2), a transition area (3) and a pipette action area (4) from bottom to top, and the tip body is formed by integrally forming.

The suction nozzle area (1) is tubular and is communicated with the liquid storage area (2), the inner diameter of the suction nozzle area (1) is 0.4 mm-0.9 mm so as to meet the requirement of high pipetting accuracy, and the vertex angle alpha of the inner cavity of the suction nozzle area (1) is 1-30 degrees. The liquid storage area (2) is communicated with the transition area (3), the inner diameter of the liquid storage area is gradually reduced from top to bottom, and the vertex angle beta and the vertex angle alpha of the inner cavity of the liquid storage area (2) are the same. The transition area (3) is communicated with the action area (4) of the liquid transfer device and comprises a first transition area (31) and a second transition area (32), the inner diameter of the first transition area (31) is smaller than that of the second transition area (32), the transition area (3) can reduce the probability of liquid entering the liquid transfer device, cross contamination is prevented, the liquid transfer device is protected, and 8 first protrusions (33) are arranged between the first transition area (31) and the second transition area (32), so that the gun head is conveniently placed in a gun head box. The action area (4) of the liquid dispenser comprises a first action area (41) and a second action area (42), the inner diameter of the first action area (41) is slightly larger than that of the second action area (42), the inner diameter of the second action area (42) is slightly larger than that of the transition area (3), 6 second bulges (43) are arranged on the outer wall of the second action area (42), the action area (4) of the liquid dispenser can be compatible with the existing liquid dispenser, and mechanical support is provided for inserting and taking out a gun head for the liquid dispenser of an automatic liquid workstation, so that the liquid storage area (2) and the suction nozzle area (1) are protected.

The inner diameter of the gun head body is gradually increased from bottom to top. The inner diameter of the liquid storage area (2) is 0.8 mm-1.8 mm. The inner diameter of the first transition zone (31) is 1/3-2/3, preferably 2/5, of the inner diameter of the second transition zone (32). The first region of action (41) has an inner diameter that is 0.5 mm-1.5 mm greater than the inner diameter of the second region of action (42). The gun head of the present invention is made of a plastic material, preferably polypropylene.

The pipette tip is set to be 30 mu L in measuring range and 0.5 mu L in pipetting accuracy through industrial design, and is suitable for a reaction system with small volume, high accuracy and high flux. And through the design to the pipettor action area, can be compatible with the pipettor of the high flux liquid workstation of current mainstream, need not redesign and build new liquid workstation, reduce cost, and easily promote.

All documents mentioned in this disclosure are incorporated by reference in this disclosure as if each were individually incorporated by reference. Further, it will be appreciated that various changes and modifications may be made by those skilled in the art after reading the above teachings, and such equivalents are intended to fall within the scope of the application as defined in the appended claims.

Claims

1. The pipette tip is characterized by comprising a tip body, wherein the tip body is integrated or integrally formed, the tip body is tubular, the tip body comprises a suction nozzle area, a liquid storage area, a transition area and a pipette action area from bottom to top, and the inner cavity of the tubular tip body is of a tapered structure from top to bottom;

The height of the suction nozzle area is 7-10mm, the vertex angle is alpha, wherein alpha is more than or equal to 1 degree and less than or equal to 30 degrees, and the inner diameter is 0.4-0.9mm;

The height of the liquid storage area is 30-35mm, the vertex angle is beta, wherein beta is more than or equal to 1 degree and less than or equal to 30 degrees, and the inner diameter is 0.8-1.8mm;

the height of the transition zone is 10-11mm, and the inner diameter is 1.8-5.4mm;

the height of the action area of the liquid transfer device is 5.4-5.8mm, and the inner diameter is 5.3-6.5mm;

A plurality of first bulges are uniformly arranged on the periphery of the transition zone;

and a plurality of second bulges are uniformly arranged on the periphery of the action area of the liquid transfer device.

2. The pipette tip of claim 1 wherein α = β.

3. The pipette tip of claim 2 wherein the transition zone comprises a first transition zone and a second transition zone, wherein the first transition zone is fixedly connected to the reservoir zone and the second transition zone is fixedly connected to the pipette action zone.

4. A pipette tip as recited in claim 3 wherein said first transition zone has an inner diameter of 1/3-2/3 of an inner diameter of said second transition zone.

5. A pipette tip as recited in claim 3 wherein an intermediate transition is provided between said first transition zone and said second transition zone.

6. The pipette tip of claim 5 wherein the pipette action zone comprises a first action zone and a second action zone, wherein the first action zone is fixedly connected with the transition zone and the second action zone is fixedly connected with the first action zone.

7. The pipette tip of claim 6 wherein the inner diameter of the first action zone is 0.5-1.5mm greater than the inner diameter of the second action zone.

8. The pipette tip of claim 6 wherein the plurality of second protrusions are uniformly disposed on the outer periphery of the second action zone.