CN110031960A - Automatic adjustment method and device - Google Patents
Automatic adjustment method and device Download PDFInfo
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- CN110031960A CN110031960A CN201910462387.5A CN201910462387A CN110031960A CN 110031960 A CN110031960 A CN 110031960A CN 201910462387 A CN201910462387 A CN 201910462387A CN 110031960 A CN110031960 A CN 110031960A
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/241—Devices for focusing
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
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Abstract
The embodiment of the present application provides a kind of Automatic adjustment method and device, the described method includes: the initial focusing point according to the first image adjusts microscope to reference state from original state, the first image is to obtain the image of measured object in the microscopical original state, and the measured object includes at least one cell；The second image of the measured object is obtained in the reference state, and is divided from the confidence for obtaining each cell at least one described cell in second image；According to the confidence of each cell point, the microscope is adjusted to dbjective state.Therefore, efficiency when microscope adjustment can be promoted to a certain extent.
Description
Technical field
This application involves technical field of data processing, and in particular to a kind of Automatic adjustment method and device.
Background technique
With the continuous development of science and technology, miniature object observe etc. using microscope more more and more universal.Tradition
When being observed by microscope object, usually have human eye by eyepiece observe, manually adjust between camera lens and glass slide
Distance, by coarse adjustment to it is substantially clear when, then be finely adjusted by vernier knob, keep the image of observed object clear
Clear imaging needs to spend longer time in microscope adjustment, although there are also the electron microscope of auto-focusing,
Auto-focusing can be achieved, camera lens and glass slide are adjusted, but in currently existing scheme, mainly use extremely complex focusing
Algorithm causes in auto-focusing, and the efficiency of system is lower.
Summary of the invention
The embodiment of the present application provides a kind of Automatic adjustment method and device, is able to ascend efficiency when microscope adjustment.
The first aspect of the embodiment of the present application provides a kind of Automatic adjustment method, is applied to microscope, wherein the side
Method includes:
Microscope is adjusted from original state to reference state, the first image according to the initial focusing point of the first image
For the image for obtaining measured object in the microscopical original state, the measured object includes at least one cell；
Obtain the second image of the measured object in the reference state, and obtain from second image it is described to
The confidence point of each cell in a few cell；
According to the confidence of each cell point, the microscope is adjusted to dbjective state.
Optionally, the microscope includes objective table and camera lens, and the original state includes that the objective table is located at initially
Stage position and the camera lens are located at initial lens location, and the reference state includes that the objective table is located at reference to loading
Platform position and the camera lens are located at reference lens position, and the initial focusing point according to the first image is by microscope from initial
State is adjusted to reference state, comprising:
The camera lens is adjusted from the initial lens location to the first lens location according to the initial focusing point, it is described
First lens location is the position that the focusing of the image of the measured object is divided into the first default focusing point, and the initial focusing point is small
In the described first default focusing point；
In first lens location, according to preset method of adjustment, by the objective table from the initial objective table position
It sets and adjusts to the first stage position；
The first focusing point of the image of the measured object is obtained in first stage position, according to first focusing
Divide and adjust the camera lens to the second lens location from first lens location, second lens location is the measured object
The focusing of image be divided into the position of the second default focusing point, the described second default focusing point is greater than the described first default focusing
Point；
In second lens location, according to the preset method of adjustment, by the objective table from first loading
Platform position is adjusted to the second stage position, and second stage position is the referenced stage position；
Second focusing point of the image of the measured object described in the referenced stage position acquisition, according to second focusing
Divide and adjusts the camera lens to the reference lens position from second lens location.
Optionally, described in first lens location, according to preset method of adjustment, by the objective table from it is described just
Beginning stage position is adjusted to the first stage position, comprising:
Obtain the reference picture of the measured object of first lens location；
It with the central point of the reference picture, is divided, is obtained as four image blocks, institute according to preset division methods
The area for stating four image blocks is identical；
Obtain the focusing point of each image block in four image blocks；
Determine that target image block, the target image block are the highest image of focusing point from four image blocks
Block；
The objective table is moved into target range along the extending direction of direction ray, obtains first objective table position
It sets, the endpoint of the direction ray is the central point of the reference picture, pair of the direction ray and the target image block
Claim overlapping of axles and far from the target image block, central point of the symmetry axis by the reference picture, the target range
The endpoint and the direction ray for being the ray for the half of the length of target segment, the target segment are with the mesh
Line segment between the intersection point of logo image block.
It is optionally, described to divide from the confidence for obtaining each cell at least one described cell in second image,
Include:
By preset network model, the posting of each cell is determined；
The confidence of the posting of each cell is allocated as the confidence point for corresponding cell.
It optionally, include multiple intermediate lens positions, institute between the initial lens location and first lens location
It states and is adjusted the camera lens to the first lens location from the initial lens location according to the initial focusing point, comprising:
According to the initial focusing point, using preset camera lens moving method, the camera lens is moved to the first intermediate mirror
Head position, first intermediate lens position be in the multiple intermediate lens position after the initial lens location and phase
Adjacent position；
The measured object is obtained in the focusing point of the middle position of the image of first intermediate lens position, and according to institute
Middle position focusing point is stated, using the preset camera lens moving method, the camera lens is moved to the second intermediate lens position,
Second intermediate lens position is after first intermediate lens position and adjacent in the multiple intermediate lens position
Position；
Using the method for above-mentioned moving lens, the camera lens is moved between the multiple intermediate lens position,
Until the camera lens is moved to first lens location.
Optionally, described according to the initial focusing point, using preset camera lens moving method, the camera lens is moved to
First intermediate lens position, comprising:
According to the initial focusing point, formula is determined according to preset step-length, determines that target adjusts step-length；
Step-length is adjusted using the target, the camera lens is moved to first intermediate lens position；
The preset step-length determines formula are as follows:
Y=k (1-0.9x),
Wherein, y is to adjust step-length, and x is focusing point, and k is adjustment factor.
Optionally, the confidence according to each cell point, the microscope is adjusted to dbjective state, comprising:
Determine that target cell, the target cell are to set at least one described cell from least one described cell
The highest cell of letter point；
The objective table is moved to target stage position, target objective table position from the referenced stage position
It is set to the position that the target cell is located at the central region of the camera lens.
Optionally, the method also includes:
The target cell is analyzed, determines the title of the measured object.
The second aspect of the embodiment of the present application provides a kind of self-checking device, is applied to microscope, described device packet
Include the first adjustment unit, acquiring unit and second adjustment unit, wherein
The first adjustment unit adjusts microscope to reference from original state according to the initial focusing point of the first image
State, the first image are to obtain the image of measured object in the microscopical original state, and the measured object includes at least
One cell；
The acquiring unit, for obtaining the second image of the measured object in the reference state, and from described
The confidence point of each cell at least one described cell is obtained in two images；
The second adjustment unit, for according to the confidence of each cell point, the microscope to be adjusted to target
State.
Optionally, the microscope includes objective table and camera lens, and the original state includes that the objective table is located at initially
Stage position and the camera lens are located at initial lens location, and the reference state includes that the objective table is located at reference to loading
Platform position and the camera lens are located at reference lens position, in the initial focusing point according to the first image by microscope from first
Beginning state is adjusted to reference state, and the first adjustment unit is specifically used for:
The camera lens is adjusted from the initial lens location to the first lens location according to the initial focusing point, it is described
First lens location is the position that the focusing of the image of the measured object is divided into the first default focusing point, and the initial focusing point is small
In the described first default focusing point；
In first lens location, according to preset method of adjustment, by the objective table from the initial objective table position
It sets and adjusts to the first stage position；
The first focusing point of the image of the measured object is obtained in first stage position, according to first focusing
Divide and adjust the camera lens to the second lens location from first lens location, second lens location is the measured object
The focusing of image be divided into the position of the second default focusing point, the described second default focusing point is greater than the described first default focusing
Point；
In second lens location, according to the preset method of adjustment, by the objective table from first loading
Platform position is adjusted to the second stage position, and second stage position is the referenced stage position；
Second focusing point of the image of the measured object described in the referenced stage position acquisition, according to second focusing
Divide and adjusts the camera lens to the reference lens position from second lens location.
Optionally, in first lens location, according to preset method of adjustment, by the objective table from the initial load
In terms of object platform position is adjusted to the first stage position, the first adjustment unit is specifically used for:
Obtain the reference picture of the measured object of first lens location；
It with the central point of the reference picture, is divided, is obtained as four image blocks, institute according to preset division methods
The area for stating four image blocks is identical；
Obtain the focusing point of each image block in four image blocks；
Determine that target image block, the target image block are the highest image of focusing point from four image blocks
Block；
The objective table is moved into target range along the extending direction of direction ray, obtains first objective table position
It sets, the endpoint of the direction ray is the central point of the reference picture, pair of the direction ray and the target image block
Claim overlapping of axles and far from the target image block, central point of the symmetry axis by the reference picture, the target range
The endpoint and the direction ray for being the ray for the half of the length of target segment, the target segment are with the mesh
Line segment between the intersection point of logo image block.
Optionally, in terms of the confidence for obtaining each cell from second image point, the acquiring unit tool
Body is used for:
By preset network model, the posting of each cell is determined；
The confidence of the posting of each cell is allocated as the confidence point for corresponding cell.
It optionally, include multiple intermediate lens positions between the initial lens location and first lens location,
It is described to be divided by the camera lens in terms of the initial lens location is adjusted to the first lens location according to the initial focusing, it is described
The first adjustment unit is specifically used for:
According to the initial focusing point, using preset camera lens moving method, the camera lens is moved to the first intermediate mirror
Head position, first intermediate lens position be in the multiple intermediate lens position after the initial lens location and phase
Adjacent position；
The measured object is obtained in the focusing point of the middle position of the image of first intermediate lens position, and according to institute
Middle position focusing point is stated, using the preset camera lens moving method, the camera lens is moved to the second intermediate lens position,
Second intermediate lens position is after first intermediate lens position and adjacent in the multiple intermediate lens position
Position；
Using the method for above-mentioned moving lens, the camera lens is moved between the multiple intermediate lens position,
Until the camera lens is moved to first lens location.
Optionally, using preset camera lens moving method, the camera lens is mobile described according to the initial focusing point
In terms of the first intermediate lens position, the first adjustment unit is specifically used for:
According to the initial focusing point, formula is determined according to preset step-length, determines that target adjusts step-length；
Step-length is adjusted using the target, the camera lens is moved to first intermediate lens position；
The preset step-length determines formula are as follows:
Y=k (1-0.9x),
Wherein, y is to adjust step-length, and x is focusing point, and k is adjustment factor.
Optionally, in the confidence according to each cell point, in terms of the microscope is adjusted to dbjective state,
The second adjustment unit is specifically used for:
Determine that target cell, the target cell are to set at least one described cell from least one described cell
The highest cell of letter point；
The objective table is moved to target stage position, target objective table position from the referenced stage position
It is set to the position that the target cell is located at the central region of the camera lens.
Optionally, the self-checking device also particularly useful for:
The target cell is analyzed, determines the title of the measured object.
The third aspect of the embodiment of the present application provides a kind of terminal, including processor, input equipment, output equipment and storage
Device, the processor, input equipment, output equipment and memory are connected with each other, wherein the memory is for storing computer
Program, the computer program include program instruction, and the processor is configured for calling described program instruction, are executed such as this
Apply for the step instruction in embodiment first aspect.
The fourth aspect of the embodiment of the present application provides a kind of computer readable storage medium, wherein above-mentioned computer can
Read the computer program that storage medium storage is used for electronic data interchange, wherein above-mentioned computer program executes computer
The step some or all of as described in the embodiment of the present application first aspect.
5th aspect of the embodiment of the present application provides a kind of computer program product, wherein above-mentioned computer program produces
Product include the non-transient computer readable storage medium for storing computer program, and above-mentioned computer program is operable to make to count
Calculation machine executes the step some or all of as described in the embodiment of the present application first aspect.The computer program product can be
One software installation packet.
Implement the embodiment of the present application, at least has the following beneficial effects:
By the way that microscope is adjusted from original state to reference state according to the initial focusing point of the first image, the first figure
As the image to obtain measured object in microscopical original state, measured object includes at least one cell, is obtained in reference state
Second image of measured object, and from the confidence of each cell obtained in the second image at least one cell point, according to every
The confidence of a cell point, microscope is adjusted to dbjective state, accordingly, with respect in existing scheme, is calculated using complicated focusing
Method is carried out the confidence point of focusing point extraction and each cell by the image to measured object, and is divided according to focusing point and confidence
Microscope is adjusted from original state to dbjective state, the required time is less when being calculated due to score, can certain journey
Time when microscope adjustment is reduced on degree, to promote efficiency when microscope adjustment.
Detailed description of the invention
In order to illustrate the technical solutions in the embodiments of the present application or in the prior art more clearly, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of application for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 provides a kind of schematic diagram of the regulating effect of Automatic adjustment method for the embodiment of the present application；
Fig. 2A provides a kind of flow diagram of Automatic adjustment method for the embodiment of the present application；
Fig. 2 B provides a kind of adjust camera lens to the first lens location from initial position for the embodiment of the present application and adjusts effect
The schematic diagram of fruit；
Fig. 2 C provides a kind of effect diagram that objective table is moved to the first stage position for the embodiment of the present application；
Fig. 2 D provides a kind of image block processing schematic for the embodiment of the present application；
Fig. 2 E is a kind of effect diagram that objective table is moved to target stage position from referenced stage position；
Fig. 3 provides the flow diagram of another Automatic adjustment method for the embodiment of the present application；
Fig. 4 provides the flow diagram of another Automatic adjustment method for the embodiment of the present application；
Fig. 5 is a kind of structural schematic diagram of terminal provided by the embodiments of the present application；
Fig. 6 provides a kind of structural schematic diagram of self-checking device for the embodiment of the present application.
Specific embodiment
Below in conjunction with the attached drawing in the embodiment of the present application, technical solutions in the embodiments of the present application carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of embodiments of the present application, instead of all the embodiments.It is based on
Embodiment in the application, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall in the protection scope of this application.
The description and claims of this application and to state term " first " in attached drawing, " second " etc. be for distinguishing not
Same object, is not use to describe a particular order.In addition, term " includes " and " having " and their any deformations, it is intended that
Non-exclusive include in covering.Such as the process, method, system, product or equipment for containing a series of steps or units do not have
It is defined in listed step or unit, but optionally further comprising the step of not listing or unit, or optionally further comprising
For other intrinsic step or units of these process, methods, product or equipment.
" embodiment " mentioned in this application is it is meant that a particular feature, structure, or characteristic described can be in conjunction with the embodiments
Included at least one embodiment of the application.The phrase, which occurs, in each position in the description might not each mean phase
Same embodiment, nor the independent or alternative embodiment with other embodiments mutual exclusion.Those skilled in the art are explicitly
Implicitly understand, embodiments described herein can be combined with other embodiments.
In order to better understand a kind of Automatic adjustment method provided by the embodiments of the present application, first below to automatic adjustment side
The progress of method is briefly introduced.Referring to Fig. 1, Fig. 1 provides a kind of regulating effect of Automatic adjustment method for the embodiment of the present application
Schematic diagram.As shown in Figure 1, by show microscope is adjusted when three kinds of states under measured object image, to automatic
Adjusting method is described, and in the initial state, the focusing point of measured object is lower, and image is more fuzzy, according to pair of the image
Coke point, focusing point can be understood as the parameter characterized to the clarity of image, and the value focused point is bigger, and image is more clear,
Conversely, the value of focusing point is smaller, image is fuzzyyer, and microscope is adjusted to reference state, and under reference state, measured object can be with
It is clearly observed, under reference state, the confidence point of each cell is calculated, the confidence point of each cell is obtained, sets
Letter point can be understood as the parameter characterized to the clarity of cell, and the highest cell of confidence point can be determined that clearest
Representative observation sample, such as in figure, the cell of confidence point 0.8 and 0.9 adjusts microscope to mesh further according to the confidence point
Mark state, under dbjective state, the highest cell of confidence point is presented on the optic centre of camera lens.Accordingly, with respect to existing scheme
It is middle that the confidence point of focusing point extraction and each cell is carried out by the image to measured object using complicated focus algorithm, and
Microscope is adjusted from original state to dbjective state according to focusing point and confidence point, when being calculated due to score the required time compared with
It is few, therefore, time when microscope adjustment can be reduced to a certain extent, to promote efficiency when microscope adjustment.
Fig. 2A is please referred to, Fig. 2A provides a kind of flow diagram of Automatic adjustment method for the embodiment of the present application.Such as figure
Shown in 2A, Automatic adjustment method includes step 201-203, specific as follows:
201, microscope is adjusted from original state to reference state according to the initial focusing point of the first image, the first image
For the image for obtaining measured object in microscopical original state, measured object includes at least one cell.
Optionally, microscope includes objective table and camera lens.Original state may include that objective table is located at initial objective table position
It sets and camera lens is located at initial lens location.Initial stage position carries it is to be understood that when glass slide is fixed on objective table
The location of object platform, for fixing measured object, initial lens location can be understood as glass slide being fixed on load glass slide
When object platform, the location of microscope.First image can be understood as at this time through measured object acquired in microscopical camera lens
Image.Measured object can be multicellular organism, or unicellular organism is set for example, middle school student's Bioexperiment is common
In on glass slide cell or biological tissue etc., be not especially limited herein.
Optionally, further include extracting initial focusing point in the embodiment of the present application, it is a kind of it is possible extract the first image just
Begin the method divided of focusing are as follows: determines model according to preset focusing point, determines that initial focus of the first image is divided.Wherein, in advance
If focusing point determine that model includes being trained to obtain to samples pictures by VGG convolutional neural networks model, wherein right
When network model is trained, positive training first is carried out to it, reverse train then is carried out to it, is finally restrained in network model
When, training is completed.The value range of focusing point is [0,1].The value of focusing point is bigger, and image is more clear, conversely, the value of focusing point
Smaller, image is fuzzyyer.
Optionally, reference state includes that objective table is located at referenced stage position and camera lens is located at reference lens position,
A kind of possible basis point method for adjusting microscope to reference state of initially focusing includes step A1-A5, specific as follows:
A1, camera lens is adjusted from initial lens location to the first lens location, the first lens location according to initial focusing point
It is divided into the position of the first default focusing point for the focusing of the image of measured object, initial focusing point is less than the first default focusing point；
Optionally, the value range of the first default focusing point can be [0.19,0.21], specifically for example can be 0.20,
0.21 etc..
It optionally, include multiple intermediate lens positions, intermediate lens position between initial lens location and the first lens location
It sets and can be understood as a middle position, i.e., the position reached after to camera adjusting target step, at the beginning of a kind of possible basis
The method that camera lens is adjusted from initial lens location to the first lens location is included step A11-A13 by beginning focusing point, specific as follows:
A11, divided according to initial focusing, using preset camera lens moving method, camera lens is moved to the first intermediate lens position
Set, the first intermediate lens position be in multiple intermediate lens positions after initial lens location and adjacent position；
Optionally, a kind of possible using preset camera lens moving method, camera lens is moved to the first intermediate lens position
Method include step A111-A112:
A111, divided according to initial focusing, determine formula according to preset step-length, determine that target adjusts step-length；
Wherein, preset step-length determines formula are as follows:
Y=k (1-0.9x),
Wherein, y is to adjust step-length, and x is focusing point, and k is adjustment factor.Adjustment factor k by empirical value or can be gone through
History data are set, it can be understood as the most fast adjustment factor determined by experiment of repeatedly focusing.According to above-mentioned tune
Save the determination formula of step-length, it can be understood as, it can determine whether out to need to carry out camera lens next time in each intermediate lens position
Mobile adjusting step-length.
A122, camera lens is moved to by the first intermediate lens position using target adjusting step-length.
In this example, step-length is adjusted by dynamically obtaining target, more accurately microscope can be adjusted, from
And accuracy when microscope is adjusted can be promoted to a certain extent.
A12, measured object is obtained in the focusing point of the middle position of the image of the first intermediate lens position, and according to interposition
Focusing point is set, using preset camera lens moving method, camera lens is moved to the second intermediate lens position, the second intermediate lens position
For in multiple intermediate lens positions after the first intermediate lens position and adjacent position；
Wherein, the method that the method for obtaining middle position focusing point specifically can refer to the initial focusing point of aforementioned acquisition.
A13, using the method for above-mentioned moving lens, camera lens is moved between multiple intermediate lens positions, until
Camera lens is moved to the first lens location.
Optionally, the mobile method of above-mentioned camera lens is repeated it is to be understood that camera lens is moved to the from initial lens location
Then one intermediate lens position is moved to the second intermediate lens position from the first intermediate lens position, by camera lens be moved to
The adjacent next intermediate lens position in intermediate lens position, that is, camera lens is moved to down from current intermediate lens position
One intermediate lens position, current lens position are adjusted at a distance from step-length between next intermediate lens position at a distance of target,
Target adjusts step-length and periodically needs to divide progress true in the focusing of the middle position of the image of current lens position according to measured object really
It is fixed, it can dynamic to determine that target adjusts step-length.The possible adjusting example of one kind please refers to Fig. 2 B, as shown in Figure 2 B, Fig. 2 B
A kind of camera lens is adjusted from initial position to the schematic diagram of the first lens location regulating effect to be provided by the embodiments of the present application.Such as
Shown in Fig. 2 B, by image in this exemplary diagram, camera adjusting is shown, when camera lens is located at initial position, focusing
It is point lower, measured object be it is fuzzy, after being adjusted by the above method, gradually reach the first lens location, the first lens location
When, the focusing point of image increases, then image is relatively clear.Different focusing point, corresponding different lens location.
In this example, camera lens is adjusted from initial position to the first lens location by the above method, using repeatedly progressive
The method of adjusting is adjusted, the shake generated when can reduce camera adjusting to a certain extent, when can promote camera lens adjustment
Accuracy and measured object adjusted image obtain when accuracy.
A2, in the first lens location, according to preset method of adjustment, objective table is adjusted from initial stage position to
One stage position；
Optionally, a kind of possible method for adjusting objective table to the first stage position from initial position includes step
A21-A25, specific as follows:
A21, obtain the first lens location measured object reference picture；
Optionally, as shown in Figure 2 C, Fig. 2 C is provided for the embodiment of the present application and a kind of objective table is moved to the first loading
The effect diagram of platform position.As shown in Figure 2 C, since reference picture is to be obtained by camera lens, camera lens is circular lens, then obtains
The image got is circular image.
A22, the central point with reference picture, are divided according to preset division methods, are obtained as four image blocks, four
The area of a image block is identical；
Optionally, the central point of reference picture, it can be understood as the center of circle of circular image.Reference picture is divided into four
Image block may refer to the division methods in Fig. 2 C.
A23, the focusing point for obtaining each image block in four image blocks；
Optionally, as shown in Figure 2 C, four image blocks can be expressed as the first image block, the second image block, third image
Block and the 4th image block.In the focusing timesharing for obtaining each image block, when being input to focusing point determination model, the image of input is only
For the image of the image block, the value of remaining image block is 0, as shown in Figure 2 D.
A24, determine that target image block, target image block are the highest image block of focusing point from four image blocks；
A25, objective table is moved into target range along the extending direction of direction ray, obtains the first stage position, mesh
Central point of the endpoint of mark ray for reference picture, symmetrical overlapping of axles and the separate target image block of ray and target image block,
Symmetry axis passes through the central point of reference picture, and target range is the half of the length of target segment, and target segment is ray
Endpoint and ray with the line segment between the intersection point of target image block.
Optionally, as shown in Figure 2 C, the moving direction of objective table is by the moving direction that identifies in figure.
In this example, by moving objective table, then measured object can be moved in the visual field of camera lens, to keep away
Exempt from that measured object is moved to except the visual field when microscope is adjusted, has led to not the feelings being observed to measured object
Condition, so as to promote accuracy when microscope is adjusted to a certain extent.
A3, the first focusing point that the image of measured object is obtained in the first stage position, divide according to the first focusing by camera lens
It adjusts from the first lens location to the second lens location, the second lens location, which is divided into second for the focusing of the image of measured object, to be preset
The position of focusing point, the second default focusing point are greater than the first default focusing point；
Wherein, the value range of the second default focusing point can be [0.69,0.71], specifically for example can be 0.70,
0.71 etc..When moving, using method with it is aforementioned camera lens is carried out it is mobile by the way of move.
A4, in the second lens location, according to preset method of adjustment, objective table is adjusted from the first stage position to
Two stage positions, the second stage position are referenced stage position；
A5, referenced stage position acquisition measured object image the second focusing point, according to the second focusing point by camera lens
It adjusts from the second lens location to reference lens position.
Optionally, method camera lens adjusted to reference lens position can refer to the method in step A1, no longer superfluous herein
It states.
In this example, microscope is adjusted first, the focusing of measured object point is reached into the first default focusing point, then right
Objective table is adjusted, and measured object is moved to the region of the highest image block of focusing point, then camera lens is adjusted, so that by
The focusing for surveying object point reaches the second default focusing point, then the position of objective table is adjusted, finally by microscope from initial shape
State is adjusted to reference state, by repeatedly detailed-oriented adjusting, can be promoted to a certain extent when microscope is adjusted
Accuracy.
202, the second image of measured object is obtained in reference state, and is obtained at least one cell from the second image
Each cell confidence point.
Optionally, a kind of method of the possible confidence for obtaining each cell point includes step B1-B2, specific as follows:
B1, pass through preset network model, determine the posting of each cell；
Wherein, preset network model can be modified faster RCNN network model.Modified faster RCNN
Network model be by the feature extraction network in traditional faster RCNN network model using mobileNet network structure into
It is obtained after row replacement.Feature extraction network is replaced by mobileNet network structure, by faster RCNN network mould
Type miniaturization makes it possible to be applied in the lesser electronic equipment of resource.
B2, the confidence of the posting of each cell is allocated as the confidence point for corresponding cell.
Wherein, after the posting for determining each cell, the corresponding Region Proposal of posting can be passed through
The confidence that Network (RPN network) is determined point obtains the confidence point of each posting.
203, according to the confidence of each cell point, microscope is adjusted to dbjective state.
Optionally, a kind of possible confidence according to each cell point, microscope is adjusted to the method packet of dbjective state
Step C1-C2 is included, specific as follows:
C1, determine that target cell, target cell are that confidence divides highest at least one cell from least one cell
Cell；
C2, objective table is moved to target stage position from referenced stage position, target stage position is target
Cell is located at the position of the central region of camera lens.
Optionally, objective table is moved to target objective table position from referenced stage position to be a kind of refering to Fig. 2 E, Fig. 2 E
The effect diagram set.As shown in Figure 2 E, highest confidence is divided into 0.9, then moving stage makes corresponding with the confidence point
Until cell is located at the central region of camera lens.
In a possible example, the embodiment of the present application can also analyze target cell, determine measured object
Title.
Optionally, determine that the method for the title of measured object can be with are as follows: in the training of the preset network model of the application
During, target is distinguished according to Bioexperiment common cell category, the confidence that posting is determined is divided into: right
Highest confidence point in the confidence of common whole cell categories point, then can be according to the cell kind corresponding to the confidence point
Class determines the type of the cell in posting.Such as during testing to measured object, " onion epidermis cell " is right
The confidence point highest answered can then determine that the classification of measured object is " onion epidermis cell ".Identifiable cell category can be with
It include being related in Bioexperiment as onion epidermis cell, Onion root-tip cell, people are blood red thin including plant cell, zooblast
Born of the same parents etc..
Referring to Fig. 3, Fig. 3 provides the flow diagram of another Automatic adjustment method for the embodiment of the present application.Such as Fig. 3
Shown, Automatic adjustment method includes step 301-308, specific as follows:
301, microscope is adjusted from original state to reference state according to the initial focusing point of the first image, the first image
For the image for obtaining measured object in microscopical original state, measured object includes at least one cell；
Wherein, above-mentioned original state includes that objective table is located at initial stage position and camera lens is located at initial camera lens position
It sets, reference state includes that objective table is located at referenced stage position and camera lens is located at reference lens position, and microscope includes carrying
Object platform and camera lens.
302, camera lens is adjusted from initial lens location to the first lens location, the first lens location according to initial focusing point
It is divided into the position of the first default focusing point for the focusing of the image of measured object；
Wherein, initial focusing point is less than the first default focusing point.
303, in the first lens location, according to preset method of adjustment, by objective table from initial stage position adjust to
First stage position；
304, the first focusing point that the image of measured object is obtained in the first stage position, divides according to the first focusing by camera lens
It adjusts from the first lens location to the second lens location, the second lens location, which is divided into second for the focusing of the image of measured object, to be preset
The position of focusing point；
Wherein, the second default focusing point is greater than the first default focusing point.
305, in the second lens location, according to preset method of adjustment, by objective table from the first stage position adjust to
Second stage position, the second stage position are referenced stage position；
306, in the second focusing point of the image of referenced stage position acquisition measured object, divided according to the second focusing by camera lens
It adjusts from the second lens location to reference lens position；
307, the second image of measured object is obtained in reference state, and is obtained at least one cell from the second image
Each cell confidence point；
308, according to the confidence of each cell point, microscope is adjusted to dbjective state.
In this example, camera lens is adjusted from initial position to the first lens location by the above method, using repeatedly progressive
The method of adjusting is adjusted, the shake generated when can reduce camera adjusting to a certain extent, when can promote camera lens adjustment
Accuracy and measured object adjusted image obtain when accuracy.
Referring to Fig. 4, Fig. 4 provides the flow diagram of another Automatic adjustment method for the embodiment of the present application.Such as Fig. 4
Shown, Automatic adjustment method includes step 401-405, specific as follows:
401, microscope is adjusted from original state to reference state according to the initial focusing point of the first image, the first image
For the image for obtaining measured object in microscopical original state, measured object includes at least one cell；
Wherein, microscope includes objective table and camera lens.
402, the second image of measured object is obtained in reference state；
403, by preset network model, the posting of each cell is determined；
Wherein, preset network model can be modified faster RCNN network model.Modified faster RCNN
Network model be by the feature extraction network in traditional faster RCNN network model using mobileNet network structure into
It is obtained after row replacement.Feature extraction network is replaced by mobileNet network structure, by faster RCNN network mould
Type miniaturization makes it possible to be applied in the lesser electronic equipment of resource.
404, the confidence of the posting of each cell is allocated as to the confidence point for corresponding cell；
405, according to the confidence of each cell point, microscope is adjusted to dbjective state.
In this example, by follow-on faster RCNN network model, it is lesser this programme can be applied to resource
In equipment, so as to promote the practicability of this programme to a certain extent.
It is consistent with above-described embodiment, referring to Fig. 5, Fig. 5 is that a kind of structure of terminal provided by the embodiments of the present application is shown
Be intended to, as shown, include processor, input equipment, output equipment and memory, processor, input equipment, output equipment and
Memory is connected with each other, wherein and for the memory for storing computer program, the computer program includes program instruction,
The processor is configured for calling described program instruction, and above procedure includes the instruction for executing following steps；
Adjusted to reference state from original state according to the initial focusing of the first image point by microscope, the first image be
Microscopical original state obtains the image of measured object, and measured object includes at least one cell；
The second image of measured object is obtained in reference state, and every at least one cell from obtaining in the second image
The confidence of a cell point；
According to the confidence of each cell point, microscope is adjusted to dbjective state.
In this example, by the way that microscope is adjusted from original state to reference shape according to the initial focusing point of the first image
State, the first image are to obtain the image of measured object in microscopical original state, and measured object includes at least one cell, is being referred to
State obtains the second image of measured object, and the confidence from each cell obtained at least one cell in the second image
Point, according to the confidence of each cell point, microscope is adjusted to dbjective state, accordingly, with respect in existing scheme, using complexity
Focus algorithm, focusing point is carried out by the image to measured object and is extracted and the confidence point of each cell, and according to focusing point
Microscope is adjusted from original state to dbjective state with confidence point, the required time is less when being calculated due to score, can
To reduce time when microscope adjustment to a certain extent, to promote efficiency when microscope adjustment.
It is above-mentioned that mainly the scheme of the embodiment of the present application is described from the angle of method side implementation procedure.It is understood that
, in order to realize the above functions, it comprises execute the corresponding hardware configuration of each function and/or software module for terminal.This
Field technical staff should be readily appreciated that, in conjunction with each exemplary unit and algorithm of embodiment description presented herein
Step, the application can be realized with the combining form of hardware or hardware and computer software.Some function actually with hardware also
It is the mode of computer software driving hardware to execute, the specific application and design constraint depending on technical solution.Profession
Technical staff can specifically realize described function to each using distinct methods, but this realization should not be recognized
For beyond scope of the present application.
The embodiment of the present application can carry out the division of functional unit according to above method example to terminal, for example, can be right
The each functional unit of each function division is answered, two or more functions can also be integrated in a processing unit.
Above-mentioned integrated unit both can take the form of hardware realization, can also realize in the form of software functional units.It needs
Illustrate, is schematical, only a kind of logical function partition to the division of unit in the embodiment of the present application, it is practical to realize
When there may be another division manner.
Consistent with the above, referring to Fig. 6, Fig. 6 provides a kind of structure of self-checking device for the embodiment of the present application
Schematic diagram.Self-checking device includes the first adjustment unit 601, acquiring unit 602 and second adjustment unit 603, wherein
The first adjustment unit 601, for according to the first image it is initial focusing point by microscope from original state adjust to
Reference state, the first image are to obtain the image of measured object in microscopical original state, and measured object includes at least one cell；
Acquiring unit 602, for reference state obtain measured object the second image, and from the second image obtain to
The confidence point of each cell in a few cell；
Second adjustment unit 603, for according to the confidence of each cell point, microscope to be adjusted to dbjective state.
Optionally, microscope includes objective table and camera lens, original state include objective table be located at initial stage position with
And camera lens is located at initial lens location, reference state includes that objective table is located at referenced stage position and camera lens is located at reference mirror
Head position adjusts microscope to reference state, the first adjustment list from original state according to the initial focusing point of the first image
Member 601 is specifically used for:
Camera lens is adjusted from initial lens location to the first lens location according to initial focusing point, the first lens location is quilt
The focusing for surveying the image of object is divided into the position of the first default focusing point, and initial focusing point is less than the first default focusing point；
In the first lens location, according to preset method of adjustment, objective table is adjusted from initial stage position to first
Stage position；
The first focusing point of the image of measured object is obtained in the first stage position, according to the first focusing point by camera lens from the
One lens location is adjusted to the second lens location, and the second lens location is that the focusing of the image of measured object is divided into the second default focusing
The position divided, the second default focusing point are greater than first and preset focusing point；
In the second lens location, according to preset method of adjustment, objective table is adjusted from the first stage position to second
Stage position, the second stage position are referenced stage position；
In the second focusing point of the image of referenced stage position acquisition measured object, according to the second focusing point by camera lens from the
Two lens locations are adjusted to reference lens position.
Optionally, in the first lens location, according to preset method of adjustment, objective table is adjusted from initial stage position
To the first stage position, the first adjustment unit 601 is specifically used for:
Obtain the reference picture of the measured object of the first lens location；
It with the central point of reference picture, is divided, is obtained as four image blocks, four figures according to preset division methods
As the area of block is identical；
Obtain the focusing point of each image block in four image blocks；
Determine that target image block, target image block are the highest image block of focusing point from four image blocks；
Objective table is moved into target range along the extending direction of direction ray, obtains the first stage position, target is penetrated
Central point of the endpoint of line for reference picture, symmetrical overlapping of axles and the separate target image block of direction ray and target image block,
Symmetry axis passes through the central point of reference picture, and target range is the half of the length of target segment, and target segment is ray
Endpoint and direction ray with the line segment between the intersection point of target image block.
Optionally, in terms of the confidence for obtaining each cell from the second image point, acquiring unit 602 is specifically used for:
By preset network model, the posting of each cell is determined；
The confidence of the posting of each cell is allocated as the confidence point for corresponding cell.
It optionally, include multiple intermediate lens positions between initial lens location and the first lens location, according to initial
By camera lens in terms of initial lens location is adjusted to the first lens location, the first adjustment unit 601 is specifically used for for focusing point:
According to initial focusing point, using preset camera lens moving method, camera lens is moved to the first intermediate lens position, the
One intermediate lens position be in multiple intermediate lens positions after initial lens location and adjacent position；
Measured object is obtained in the focusing point of the middle position of the image of the first intermediate lens position, and according to middle position pair
Coke point, using preset camera lens moving method, is moved to the second intermediate lens position for camera lens, the second intermediate lens position is more
In a intermediate lens position after the first intermediate lens position and adjacent position；
Using the method for above-mentioned moving lens, camera lens is moved between multiple intermediate lens positions, until by mirror
Head is moved to the first lens location.
Optionally, according to initial focusing point, using preset camera lens moving method, camera lens is moved to the first intermediate mirror
In terms of head position, the first adjustment unit 601 is specifically used for:
According to initial focusing point, formula is determined according to preset step-length, determines that target adjusts step-length；
Step-length is adjusted using target, camera lens is moved to the first intermediate lens position；
Preset step-length determines formula are as follows:
Y=k (1-0.9x),
Wherein, y is to adjust step-length, and x is focusing point, and k is adjustment factor.
Optionally, according to the confidence of each cell point, in terms of microscope is adjusted to dbjective state, second adjustment unit
602 are specifically used for:
Determine that target cell, target cell are that confidence point is highest thin at least one cell from least one cell
Born of the same parents；
Objective table is moved to target stage position from referenced stage position, target stage position is target cell
Positioned at the position of the central region of camera lens.
Optionally, self-checking device also particularly useful for:
Target cell is analyzed, determines the title of measured object.
The embodiment of the present application also provides a kind of computer storage medium, wherein computer storage medium storage is for electricity
The computer program of subdata exchange, it is as any in recorded in above method embodiment which execute computer
A kind of some or all of Automatic adjustment method step.
The embodiment of the present application also provides a kind of computer program product, and the computer program product includes storing calculating
The non-transient computer readable storage medium of machine program, the computer program make computer execute such as above method embodiment
Some or all of any Automatic adjustment method of middle record step.
It should be noted that for the various method embodiments described above, for simple description, therefore, it is stated as a series of
Combination of actions, but those skilled in the art should understand that, the application is not limited by the described action sequence because
According to the application, some steps may be performed in other sequences or simultaneously.Secondly, those skilled in the art should also know
It knows, the embodiments described in the specification are all preferred embodiments, related actions and modules not necessarily the application
It is necessary.
In the above-described embodiments, it all emphasizes particularly on different fields to the description of each embodiment, there is no the portion being described in detail in some embodiment
Point, reference can be made to the related descriptions of other embodiments.
In several embodiments provided herein, it should be understood that disclosed device, it can be by another way
It realizes.For example, the apparatus embodiments described above are merely exemplary, such as the division of the unit, it is only a kind of
Logical function partition, there may be another division manner in actual implementation, such as multiple units or components can combine or can
To be integrated into another system, or some features can be ignored or not executed.Another point, shown or discussed is mutual
Coupling, direct-coupling or communication connection can be through some interfaces, the indirect coupling or communication connection of device or unit,
It can be electrical or other forms.
The unit as illustrated by the separation member may or may not be physically separated, aobvious as unit
The component shown may or may not be physical unit, it can and it is in one place, or may be distributed over multiple
In network unit.It can select some or all of unit therein according to the actual needs to realize the mesh of this embodiment scheme
's.
It, can also be in addition, applying for that each functional unit in bright each embodiment can integrate in one processing unit
It is that each unit physically exists alone, can also be integrated in one unit with two or more units.Above-mentioned integrated list
Member both can take the form of hardware realization, can also be realized in the form of software program module.
If the integrated unit is realized in the form of software program module and sells or use as independent product
When, it can store in a computer-readable access to memory.Based on this understanding, the technical solution of the application substantially or
Person says that all or part of the part that contributes to existing technology or the technical solution can body in the form of software products
Reveal and, which is stored in a memory, including some instructions are used so that a computer equipment
(can be personal computer, server or network equipment etc.) executes all or part of each embodiment the method for the application
Step.And memory above-mentioned includes: USB flash disk, read-only memory (read-only memory, ROM), random access memory
The various media that can store program code such as (random access memory, RAM), mobile hard disk, magnetic or disk.
Those of ordinary skill in the art will appreciate that all or part of the steps in the various methods of above-described embodiment is can
It is completed with instructing relevant hardware by program, which can store in a computer-readable memory, memory
It may include: flash disk, read-only memory, random access device, disk or CD etc..
The embodiment of the present application is described in detail above, specific case used herein to the principle of the application and
Embodiment is expounded, the description of the example is only used to help understand the method for the present application and its core ideas；
At the same time, for those skilled in the art can in specific embodiments and applications according to the thought of the application
There is change place, in conclusion the contents of this specification should not be construed as limiting the present application.
Claims (10)
1. a kind of Automatic adjustment method characterized by comprising
Microscope is adjusted from original state to reference state according to the initial focusing point of the first image, the first image be
The microscopical original state obtains the image of measured object, and the measured object includes at least one cell；
The second image of the measured object is obtained in the reference state, and obtains described at least one from second image
The confidence of each cell in a cell point；
According to the confidence of each cell point, the microscope is adjusted to dbjective state.
2. described initial the method according to claim 1, wherein the microscope includes objective table and camera lens
State includes that the objective table is located at initial stage position and the camera lens is located at initial lens location, the reference state
It is located at referenced stage position including the objective table and the camera lens is located at reference lens position, it is described according to the first image
Initial focusing point microscope is adjusted from original state to reference state, comprising:
The camera lens is adjusted from the initial lens location to the first lens location according to the initial focusing point, described first
Lens location is the position that the focusing of the image of the measured object is divided into the first default focusing point, and the initial focusing point is less than institute
State the first default focusing point；
In first lens location, according to preset method of adjustment, by the objective table from the initial stage position tune
It is whole to the first stage position；
The first focusing point of the image of the measured object is obtained in first stage position, it will according to first focusing point
The camera lens is adjusted from first lens location to the second lens location, and second lens location is the figure of the measured object
The focusing of picture is divided into the position of the second default focusing point, and the described second default focusing point is greater than the described first default focusing point；
In second lens location, according to the preset method of adjustment, by the objective table from first objective table position
It sets and adjusts to the second stage position, second stage position is the referenced stage position；
Second focusing point of the image of the measured object described in the referenced stage position acquisition, will according to second focusing point
The camera lens is adjusted from second lens location to the reference lens position.
3. according to the method described in claim 2, it is characterized in that, described in first lens location, according to preset tune
Adjusting method adjusts the objective table to the first stage position from the initial stage position, comprising:
Obtain the reference picture of the measured object of first lens location；
It with the central point of the reference picture, is divided, is obtained as four image blocks, described four according to preset division methods
The area of a image block is identical；
Obtain the focusing point of each image block in four image blocks；
Determine that target image block, the target image block are the highest image block of focusing point from four image blocks；
The objective table is moved into target range along the extending direction of direction ray, obtains first stage position, institute
The endpoint for stating direction ray is the central point of the reference picture, the symmetry axis weight of the direction ray and the target image block
It closes and the separate target image block, the symmetry axis passes through the central point of the reference picture, the target range is target
The half of the length of line segment, the endpoint and the direction ray that the target segment is the ray are with the target image
Line segment between the intersection point of block.
4. according to the method in claim 2 or 3, which is characterized in that the initial lens location and first camera lens position
Include multiple intermediate lens positions between setting, it is described according to the initial focusing point by the camera lens from the initial lens location
It adjusts to the first lens location, comprising:
According to the initial focusing point, using preset camera lens moving method, the camera lens is moved to the first intermediate lens position
It sets, first intermediate lens position is after the initial lens location and adjacent in the multiple intermediate lens position
Position；
The measured object is obtained in the focusing point of the middle position of the image of first intermediate lens position, and according in described
Between position focusing point, using the preset camera lens moving method, the camera lens is moved to the second intermediate lens position, it is described
Second intermediate lens position be in the multiple intermediate lens position after first intermediate lens position and adjacent position
It sets；
Using the method for above-mentioned moving lens, the camera lens is moved between the multiple intermediate lens position, until
The camera lens is moved to first lens location.
5. a kind of self-checking device, which is characterized in that be applied to microscope, described device includes the first adjustment unit, obtains
Unit and second adjustment unit, wherein
The first adjustment unit adjusts microscope to reference shape from original state according to the initial focusing point of the first image
State, the first image are to obtain the image of measured object in the microscopical original state, and the measured object includes at least one
A cell；
The acquiring unit, for obtaining the second image of the measured object in the reference state, and from second figure
The confidence point of each cell at least one described cell is obtained as in；
The second adjustment unit, for according to the confidence of each cell point, the microscope to be adjusted to dbjective state.
6. device according to claim 5, which is characterized in that the microscope includes objective table and camera lens, described initial
State includes that the objective table is located at initial stage position and the camera lens is located at initial lens location, the reference state
It is located at referenced stage position including the objective table and the camera lens is located at reference lens position, described according to the first figure
The initial focusing point of picture adjusts microscope to reference state from original state, and the first adjustment unit is specifically used for:
The camera lens is adjusted from the initial lens location to the first lens location according to the initial focusing point, described first
Lens location is the position that the focusing of the image of the measured object is divided into the first default focusing point, and the initial focusing point is less than institute
State the first default focusing point；
In first lens location, according to preset method of adjustment, by the objective table from the initial stage position tune
It is whole to the first stage position；
The first focusing point of the image of the measured object is obtained in first stage position, it will according to first focusing point
The camera lens is adjusted from first lens location to the second lens location, and second lens location is the figure of the measured object
The focusing of picture is divided into the position of the second default focusing point, and the described second default focusing point is greater than the described first default focusing point；
In second lens location, according to the preset method of adjustment, by the objective table from first objective table position
It sets and adjusts to the second stage position, second stage position is the referenced stage position；
Second focusing point of the image of the measured object described in the referenced stage position acquisition, will according to second focusing point
The camera lens is adjusted from second lens location to the reference lens position.
7. device according to claim 6, which is characterized in that in first lens location, according to preset adjustment side
Method, by the objective table in terms of the initial stage position is adjusted to the first stage position, the first adjustment unit
It is specifically used for:
Obtain the reference picture of the measured object of first lens location；
It with the central point of the reference picture, is divided, is obtained as four image blocks, described four according to preset division methods
The area of a image block is identical；
Obtain the focusing point of each image block in four image blocks；
Determine that target image block, the target image block are the highest image block of focusing point from four image blocks；
The objective table is moved into target range along the extending direction of direction ray, obtains first stage position, institute
The endpoint for stating direction ray is the central point of the reference picture, the symmetry axis weight of the direction ray and the target image block
It closes and the separate target image block, the symmetry axis passes through the central point of the reference picture, the target range is target
The half of the length of line segment, the endpoint and the direction ray that the target segment is the ray are with the target image
Line segment between the intersection point of block.
8. device according to claim 6 or 7, which is characterized in that the initial lens location and first camera lens position
Include multiple intermediate lens positions between setting, it is described according to the initial focusing point by the camera lens from the initial camera lens position
It sets in terms of adjusting to the first lens location, the first adjustment unit is specifically used for:
According to the initial focusing point, using preset camera lens moving method, the camera lens is moved to the first intermediate lens position
It sets, first intermediate lens position is after the initial lens location and adjacent in the multiple intermediate lens position
Position；
The measured object is obtained in the focusing point of the middle position of the image of first intermediate lens position, and according in described
Between position focusing point, using the preset camera lens moving method, the camera lens is moved to the second intermediate lens position, it is described
Second intermediate lens position be in the multiple intermediate lens position after first intermediate lens position and adjacent position
It sets；
Using the method for above-mentioned moving lens, the camera lens is moved between the multiple intermediate lens position, until
The camera lens is moved to first lens location.
9. a kind of terminal, which is characterized in that the processor, defeated including processor, input equipment, output equipment and memory
Enter equipment, output equipment and memory to be connected with each other, wherein the memory is for storing computer program, the computer
Program includes program instruction, and the processor is configured for calling described program instruction, is executed such as any one of claim 1-4
The method.
10. a kind of computer readable storage medium, which is characterized in that the computer storage medium is stored with computer program,
The computer program includes program instruction, and described program instruction makes the processor execute such as right when being executed by a processor
It is required that the described in any item methods of 1-4.
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