CN118210138A - A slide detection and positioning system and method based on digital application - Google Patents

Abstract

The invention discloses a slide glass detection positioning system and method based on digital application, and belongs to the technical field of big data. The invention installs a positioning beam source on a slide glass detection table, and carries out initial detection positioning on the slide glass by the positioning beam source, and carries out real-time transmission on positioning data to a detection control unit by sensing equipment; the detection control unit carries out space evaluation on the placement square matrix of the current slide according to the initial positioning data of the slide, analyzes the offset degree of the initial position, and carries out self-adaptive correction regulation and control on the slide according to the analysis result; carrying out data scanning extraction on the slide after the correction, carrying out dynamic programming adjustment on a data extraction scanning path of a slide detector by combining slide positioning correction data with slide scanning data, and carrying out difference item processing on the scanning data; and storing slide positioning data, slide detection path data corresponding to the slide detector and scanning data, and outputting the scanning data.

Description

A slide detection and positioning system and method based on digital application

Technical Field

The present invention relates to the field of big data technology, and in particular to a slide detection and positioning system and method based on digital applications.

Background technique

A slide is a piece of glass or quartz used for microscopic observation in the laboratory. It can be used to carry and fix samples that need to be observed. Data can be recorded on the slide through special coating or printing technology. It is widely used in biology, medicine, pathology, histology and other fields.

Under the existing technology, people can replace manual observation and data analysis of slides to a certain extent through automatic slide detectors; however, the instrument relies heavily on the correct spatial placement of the slides; this often leads to deviations or errors in the placement of the slides due to negligence when the slides are placed manually, which will cause omissions or inability to scan the observation target when the instrument scans the data on the slide; this will cause great interference to the slide observation work.

Summary of the invention

The object of the present invention is to provide a slide detection and positioning system and method based on digital application to solve the problems raised in the above background technology.

In order to solve the above technical problems, the present invention provides the following technical solutions:

A method for detecting and positioning a glass slide based on digital application, the method comprising the following steps:

S100, installing a positioning beam source on a glass slide detection table, performing initial detection and positioning of the glass slide by the positioning beam source, and transmitting the positioning data to the inspection and control unit in real time by a sensor device;

S200, the inspection and control unit performs a spatial evaluation on the placement matrix of the current glass slide according to the initial positioning data of the glass slide, analyzes the offset degree of the initial position, and performs adaptive position correction control on the glass slide according to the analysis result;

S300, scanning and extracting data from the corrected slide, dynamically planning and adjusting the data extraction scanning path of the slide detector through the slide positioning correction data combined with the slide scanning data, and performing difference processing on the scanning data;

S400, storing the slide positioning data, the slide detection path data corresponding to the slide detector and the scan data, and outputting the scan data.

The S100 installs a positioning beam source on the glass slide detection table, performs initial detection and positioning of the glass slide through the positioning beam source, and transmits the positioning data to the inspection and control unit in real time through the sensor device:

S101, pre-installing a positioning beam source on a glass slide detection platform, wherein the glass slide detection platform is composed of a glass slide carrying table and a microscope detector; wherein the positioning beam source is installed on both the glass slide carrying table and the microscope detector respectively; the positioning beam source emits a group of uniform weak laser beams for positioning detection;

S102. In the two-dimensional plane of the glass slide supporting table, randomly select a horizontal side and any adjacent vertical side to position the light beam source; detect the initial position data of the glass slide by translating the positioning light beam source on the horizontal side and the adjacent vertical side, collect the detection data and transmit it to the inspection and control center in real time through the sensor equipment; take the scanning center point of the microscope detector as the correction point, use the correction point as a reference point, position the light beam source around the scanning lens of the microscope detector, perform positioning detection on the scanning position of the microscope detector on the glass slide, collect the detection data and transmit it to the inspection and control center in real time through the sensor equipment; the sensor equipment is an IoT sensor.

The S200 inspection and control unit performs a spatial evaluation on the current placement matrix of the glass slide according to the initial positioning data of the glass slide, analyzes the degree of deviation of the initial position, and performs adaptive position correction and control on the glass slide according to the analysis results as follows:

S201, the inspection and control center receives the initial positioning data of the glass slide, and evaluates the spatial position of the placement matrix of the glass slide according to the initial positioning data of the glass slide, and the evaluation steps are as follows:

S201-1, using the positioning beam source installed on the slide carrier to determine the side edge of the slide, and using the vertical angle relationship between the two positioning beam sources to digitally determine the plane position of the slide;

S201-2, constructing a spatial cloud coordinate system for the glass slide carrier through projection, taking the two positioning beams of the glass slide carrier as the dynamic coordinate base axes, and obtaining the offset triangle between the initial position of the current glass slide and the two positioning beams by translating the positioning beams from the starting point to the glass slide; the starting point is any end point on the side of the positioning beam source of the device on the glass slide carrier;

S201-3, calculating the offset angle of the initial position of the current slide by offsetting the triangle, and the calculation formula is: ; where /> is the offset angle, /> is the length of the hypotenuse of the offset triangle, /> is the length of the vertical side adjacent to the offset angle; since the positioning beams are in a vertical relationship, the offset triangle constructed is a right triangle, and when taking the offset angle, the smaller acute angle of the two acute angles is taken as the offset angle, because when performing position correction, the offset position of the slide can be adjusted with fewer correction operations;

S201-4, by evaluating the initial position deviation angle of the current slide on the slide carrier plane, if , the slides are manually repositioned and the initial position is re-analyzed; if /> , then the slide carrier automatic correction system will perform adaptive correction operation on the current slide offset; if /> , then the initial position of the slide does not need to be corrected; where /> Preset offset angles for the system;

S202, obtaining the coordinates of the positioning point of the positioning beam on the plane of the glass slide carrier by emitting a positioning beam to the glass slide through a positioning beam source installed around the scanning lens of the microscope detector , by determining the spatial dislocation of the coordinates, it is determined whether to perform a correction operation on the current microscope detector position. The judgment method is whether the current microscope detector observation point a exists in the slide position matrix; if /> , then the current microscope detector observation point exists in the slide position matrix, and there is no need to perform position correction on the microscope detector; if/> , then the current microscope detector observation point does not exist in the slide position matrix, and the microscope detector is corrected; where the coordinates of observation point a are / > or/> or/> , the slide position matrix A is/> ; where i is the distance from the positioning beam source around the scanning lens of the microscope detector to the center of the scanning lens, /> 、/> , and/> are the coordinates of the edge endpoints of the slide respectively; the observation point coordinates are determined according to the position of the positioning beam source device, so although its coordinates are unique, there are many situations when the position is uncertain; the slide position matrix is a position matrix constructed by the coordinates of the four endpoints of the slide, because the remaining coordinates are within these four endpoints;

S203, correcting the initial offset position of the glass slide by means of a mechanical clamping and pushing device installed on the side of the glass slide carrier; the mechanical clamping and pushing device is folded and stored at the edge of the glass slide carrier, and when the position of the glass slide is corrected, it is released and the glass slide is fixed and the offset angle is adjusted by using its terminal clamping arm; the observation point of the glass slide is adjusted by moving the position of the observation microscope of the microscope detector; the top of the observation microscope of the microscope detector is connected to a connecting rod that supports extension and steering.

The steps of performing data scanning and extraction on the corrected slide in S300, dynamically planning and adjusting the data extraction scanning path of the slide detector by combining the slide positioning correction data with the slide scanning data, and performing difference processing on the scanning data are as follows:

S301, after the slide and the observation microscope are aligned, the slide is scanned and data is extracted using the observation microscope; the coordinates of the current observation point are obtained in the coordinate plane of the slide carrier and the coordinate matrix corresponding to the slide observation target/> ; By judging whether the coordinates of the current observation point exist in the coordinate matrix of the target observed on the slide; if so, the coordinates of the current observation point are used as the starting point of the microscope data scan, and the coordinate matrix of the current observation target is fully scanned using the adjacency traversal method; if not, the distance between the current observation point and the observation target is calculated, and the calculation formula is/> ; Where v is the distance between the current observation point and the observation target; take/> The minimum translation distance is used to translate the current observation point to the coordinate matrix of the observation target. The coordinates of the current observation point are used as the starting point of the microscope data scanning, and the coordinate matrix of the current observation target is fully scanned by using the adjacent traversal method; the adjacent traversal method is to use the coordinates of the current point as the starting point, analyze and calculate the adjacent coordinate points, and generate a movement path that traverses all coordinate points in the target area without duplication;

S302, traversing the coordinate matrix of the observation target on the slide through the microscope, and extracting the observation target image data at each coordinate point; marking the extracted image data according to the corresponding observation target coordinates, and transmitting the processed image data to the graphic analysis unit;

S303, in the graphic analysis unit, the received image data is restored to relative spatial position according to its corresponding coordinate data; the restored image data is translated and spliced through difference processing, the repeated image data in adjacent pictures are compared and fused, and the pixel points in the overlapped area of the image are calculated by data dimensionality reduction, and the calculation formula is: ; where /> is the pixel coordinate in the image/> The pixel value after dimension reduction, is the pixel coordinate in the image/> Compare the pixel values of two adjacent images after fusion and coverage, /> is the pixel coordinate in the image/> The pixel values before the two adjacent images are compared and fused; since the target observed on the slide is fully scanned, the target image data collected by the scan will have repeated parts in most cases. When analyzing and observing these repeated data, the amount of analysis and calculation will increase and there will be data interference. Therefore, the repeated parts can be covered and the data changes of the corresponding pixels before and after can be used for dimensionality reduction calculation to reduce interference;

S304, by performing image data splicing and dimensionality reduction operations on all collected images, the collected multi-image data are merged into comprehensive microscopic image data of the slide observation target.

The S400 stores the slide positioning data, the slide detection path data corresponding to the slide detector and the scan data, and outputs the scan data:

S401, outputting the comprehensive microscopic image data of the slide observation target to a port;

S402, using the time point as an index, storing the current slide positioning data, the slide detection path data corresponding to the slide detector, the scanning data and the image analysis data in a diary period.

A slide detection and positioning system based on digital application, the system comprises a positioning data acquisition module, a detection and control center, a slide detection data analysis module and a multi-data storage and feedback module;

The positioning data acquisition module performs initial detection and positioning of the slide through the positioning beam source of the slide detection table device, and transmits the positioning data to the inspection and control unit in real time through the sensor equipment; the inspection and control center performs spatial evaluation of the placement matrix of the current slide according to the initial positioning data of the slide, analyzes the degree of offset of the initial position, and performs adaptive correction and regulation of the slide according to the analysis result; the slide detection data analysis module performs data scanning and extraction on the corrected slide, and dynamically plans and adjusts the data extraction scanning path of the slide detector and performs difference processing on the scanning data through the slide positioning correction data combined with the slide scanning data; the multi-data storage feedback module stores the slide positioning data, the slide detection path data corresponding to the slide detector and the scanning data, and outputs the scanning data.

The positioning data acquisition module includes a glass slide positioning data acquisition unit and a data transmission unit; the glass slide positioning data acquisition unit pre-installs a positioning beam source on a glass slide detection platform, and the glass slide detection platform is composed of a glass slide carrying table and a microscope detector; wherein the positioning beam source is installed on both the glass slide carrying table and the microscope detector respectively; the positioning beam source emits a group of uniform weak laser beams for positioning detection; in the two-dimensional plane of the glass slide carrying table, any horizontal side and any adjacent vertical side are installed with the positioning beam source; the initial position data of the glass slide is detected and collected by translating the positioning beam source on the horizontal side and its adjacent vertical side; with the scanning center point of the microscope detector as the correction point, by using the correction point as a reference point, the positioning beam source is installed around the scanning lens of the microscope detector, and the scanning position and orientation of the microscope detector on the glass slide are positioned, detected and collected; the data transmission unit transmits the collected data to the inspection and control center in real time through the sensing device; the sensing device is an IoT sensor.

The inspection and control center includes a spatial array evaluation unit, an initial position offset analysis unit and an adaptive position correction control unit; the spatial array evaluation unit uses the positioning beam source installed on the slide carrier to determine the side edge of the slide, and uses the vertical angle relationship between the two positioning beam sources to digitally determine the plane position of the slide; the slide carrier is constructed into a spatial cloud coordinate system through projection, and the two positioning beams of the slide carrier are used as the dynamic coordinate base axis, and the initial position of the current slide and the offset triangle constructed by the two positioning beams are obtained by translating the positioning beam from the starting point to the slide; the starting point is any end point on the side of the positioning beam source installed on the slide carrier; the offset angle of the initial position of the current slide is calculated through the offset triangle; the initial position offset angle of the current slide on the plane of the slide carrier is evaluated; the initial position offset analysis unit uses the positioning beam source installed around the scanning lens of the microscope detector to emit the positioning beam from the slide The method comprises the following steps: positioning the light beam, obtaining the coordinates of the positioning point of the positioning light beam on the plane of the glass slide carrier, determining the spatial dislocation of the coordinates, judging whether to perform a correction operation on the current position of the microscope detector, and judging whether the current observation point of the microscope detector exists in the glass slide position matrix; the adaptive correction control unit judges the correction demand for the initial position of the glass slide and the observation microscope according to the evaluation results of the above-mentioned spatial matrix and the initial position offset analysis results, and performs corresponding correction operations according to the judgment results; the initial offset position of the glass slide is corrected by a mechanical clamping and pushing device installed on the side of the glass slide carrier; the mechanical clamping and pushing device is folded and stored at the edge of the glass slide carrier, and when the slide position is corrected, it is released and its terminal clamping arm is used to fix and adjust the offset angle of the glass slide; the observation point of the glass slide is adjusted by moving the position of the observation microscope of the microscope detector; the top of the observation microscope of the microscope detector is connected with a connecting rod that supports extension and steering.

The slide detection data analysis module includes a dynamic scanning path planning unit and a graphic analysis unit; after the dynamic scanning path planning unit completes the alignment of the slide and the observation microscope, the observation microscope is used to scan and extract data from the slide; the coordinates of the current observation point and the coordinate matrix of the corresponding slide observation target are obtained in the coordinate plane of the slide carrier; by judging whether the coordinates of the current observation point exist in the coordinate matrix of the slide observation target; if so, the coordinates of the current observation point are used as the starting point of the microscope data scan, and the coordinate matrix of the current observation target is fully scanned by the adjacency traversal method; if not, the distance between the current observation point and the observation target is calculated; the minimum translation distance is taken, and the current observation point is translated to the coordinate matrix of the observation target, and the coordinates of the current observation point are used as the starting point of the microscope data scan, and the coordinate matrix of the current observation target is fully scanned by the adjacency traversal method; the adjacency traversal method The method is as follows: taking the coordinates of the current point as the starting point, analyzing and calculating the adjacent coordinate points, generating a moving path that traverses all coordinate points in the target area without duplication and in full; traversing the coordinate matrix of the target observed on the slide through a microscope, and extracting the image data of the observed target at each coordinate point; marking the extracted image data according to the corresponding coordinates of the observed target, and transmitting the processed image data to a graphic analysis unit; the graphic analysis unit restores the relative spatial position of the received image data according to its corresponding coordinate data; using difference processing to translate and splice the restored image data, compare and fuse the repeated image data in the adjacent pictures, and perform data dimensionality reduction calculation on the pixel points in the overlapping coverage area of the image; by performing image data splicing and dimensionality reduction operations on the collected images, the collected multiple image data are fused into comprehensive microscopic image data of the target observed on the slide.

The multi-data storage feedback module includes a target microscopic image data output unit and a full analysis data diary storage unit; the target microscopic image data output unit outputs the comprehensive microscopic image data of the slide observation target through a port; the full analysis data diary storage unit uses the time point as an index to store the current slide positioning data, the slide detection path data corresponding to the slide detector, the scanning data and the image analysis data in a diary period.

Compared with the prior art, the beneficial effects achieved by the present invention are as follows: the present invention realizes the whole process of data observation, scanning and analysis of the glass slide through the action of multiple modules; the present invention has an additional positioning detection procedure before observing the glass slide, and can avoid the observation failure caused by artificially induced problems to a certain extent by performing initial position self-detection and adaptive position correction on the glass slide and the observation microscope; secondly, the present invention can realize full data scanning through dynamic planning route in the subsequent slide observation target image data extraction and processing process, and can perform dimensionality reduction processing on the scanned data, so as to present the image data of the observation target to the greatest extent while reducing the amount of analysis data and interference.

BRIEF DESCRIPTION OF THE DRAWINGS

The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

FIG1 is a schematic structural diagram of a slide detection and positioning system based on digital application according to the present invention.

Detailed ways

The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

Please refer to Figure 1, the present invention provides a technical solution:

A method for detecting and positioning a glass slide based on digital application, the method comprising the following steps:

S100, installing a positioning beam source on a glass slide detection table, performing initial detection and positioning of the glass slide by the positioning beam source, and transmitting the positioning data to the inspection and control unit in real time by a sensor device;

S200, the inspection and control unit performs a spatial evaluation on the placement array of the current glass slide according to the initial positioning data of the glass slide, analyzes the degree of deviation of the initial position, and performs adaptive position correction control on the glass slide according to the analysis result;

S300, scanning and extracting data from the corrected slide, dynamically planning and adjusting the data extraction scanning path of the slide detector through the slide positioning correction data combined with the slide scanning data, and performing difference processing on the scanning data;

S400, storing the slide positioning data, the slide detection path data corresponding to the slide detector and the scan data, and outputting the scan data.

The S100 installs a positioning beam source on the glass slide detection table, performs initial detection and positioning of the glass slide by the positioning beam source, and transmits the positioning data to the inspection and control unit in real time by the sensor device:

S101, pre-installing a positioning beam source on a glass slide detection platform, wherein the glass slide detection platform is composed of a glass slide carrying table and a microscope detector; wherein the positioning beam source is installed on both the glass slide carrying table and the microscope detector respectively; the positioning beam source emits a group of uniform weak laser beams for positioning detection;

S102. In the two-dimensional plane of the glass slide supporting table, randomly select a horizontal side and any adjacent vertical side to position the light beam source; detect the initial position data of the glass slide by translating the positioning light beam source on the horizontal side and the adjacent vertical side, collect the detection data and transmit it to the inspection and control center in real time through the sensor equipment; take the scanning center point of the microscope detector as the correction point, use the correction point as a reference point, position the light beam source around the scanning lens of the microscope detector, perform positioning detection on the scanning position of the microscope detector on the glass slide, collect the detection data and transmit it to the inspection and control center in real time through the sensor equipment; the sensor equipment is an IoT sensor.

The S200 inspection and control unit performs a spatial evaluation on the current placement matrix of the glass slide according to the initial positioning data of the glass slide, analyzes the degree of deviation of the initial position, and performs adaptive position correction and control on the glass slide according to the analysis results as follows:

S201, the inspection and control center receives the initial positioning data of the glass slide, and evaluates the spatial position of the placement matrix of the glass slide according to the initial positioning data of the glass slide, and the evaluation steps are as follows:

S201-1, using the positioning beam source installed on the slide carrier to determine the side edge of the slide, and using the vertical angle relationship between the two positioning beam sources to digitally determine the plane position of the slide;

S201-2, constructing a spatial cloud coordinate system for the glass slide carrier through projection, taking the two positioning beams of the glass slide carrier as the dynamic coordinate base axes, and obtaining the offset triangle between the initial position of the current glass slide and the two positioning beams by translating the positioning beams from the starting point to the glass slide; the starting point is any end point on the side of the positioning beam source of the device on the glass slide carrier;

S201-3, calculating the offset angle of the initial position of the current slide by offsetting the triangle, and the calculation formula is: ; where /> is the offset angle, /> is the length of the hypotenuse of the offset triangle, /> is the length of the vertical side adjacent to the offset angle;

S201-4, by evaluating the initial position deviation angle of the current slide on the slide carrier plane, if , the slides are manually repositioned and the initial position is re-analyzed; if /> , the slide carrier automatic correction system will perform adaptive correction operation on the current slide offset; if /> , then the initial position of the slide does not need to be corrected; where /> Preset offset angles for the system;

S202, using a positioning beam source installed around the scanning lens of the microscope detector to emit a positioning beam to the glass slide, and obtaining the coordinates of the positioning point of the positioning beam on the plane of the glass slide carrier , by determining the spatial dislocation of the coordinates, it is determined whether to perform a correction operation on the current microscope detector position. The judgment method is whether the current microscope detector observation point a exists in the slide position matrix; if /> , then the current microscope detector observation point exists in the slide position matrix, and there is no need to perform position correction on the microscope detector; if/> , then the current microscope detector observation point does not exist in the slide position matrix, and the microscope detector is corrected; where the coordinates of observation point a are / > or/> or/> , the slide position matrix A is/> ; where i is the distance from the positioning beam source around the scanning lens of the microscope detector to the center of the scanning lens, /> 、/> , and/> are the coordinates of the end points of the edge of the slide respectively;

S203, correcting the initial offset position of the glass slide by means of a mechanical clamping and pushing device installed on the side of the glass slide carrier; the mechanical clamping and pushing device is folded and stored at the edge of the glass slide carrier, and when the position of the glass slide is corrected, it is released and the glass slide is fixed and the offset angle is adjusted by using its terminal clamping arm; the observation point of the glass slide is adjusted by moving the position of the observation microscope of the microscope detector; the top of the observation microscope of the microscope detector is connected to a connecting rod that supports extension and steering.

The steps of performing data scanning and extraction on the corrected slide in S300, dynamically planning and adjusting the data extraction scanning path of the slide detector by combining the slide positioning correction data with the slide scanning data, and performing difference processing on the scanning data are as follows:

S301, after the slide and the observation microscope are aligned, the slide is scanned and data is extracted using the observation microscope; the coordinates of the current observation point are obtained in the coordinate plane of the slide carrier and the coordinate matrix corresponding to the slide observation target/> ; By judging whether the coordinates of the current observation point exist in the coordinate matrix of the target observed on the slide; if so, the coordinates of the current observation point are used as the starting point of the microscope data scan, and the coordinate matrix of the current observation target is fully scanned using the adjacency traversal method; if not, the distance between the current observation point and the observation target is calculated, and the calculation formula is/> ; Where v is the distance between the current observation point and the observation target; take/> The minimum translation distance is used to translate the current observation point to the coordinate matrix of the observation target. The coordinates of the current observation point are used as the starting point of the microscope data scanning, and the coordinate matrix of the current observation target is fully scanned by using the adjacent traversal method; the adjacent traversal method is to use the coordinates of the current point as the starting point, analyze and calculate the adjacent coordinate points, and generate a movement path that traverses all coordinate points in the target area without duplication;

S302, traversing the coordinate matrix of the observation target on the slide through the microscope, and extracting the observation target image data at each coordinate point; marking the extracted image data according to the corresponding observation target coordinates, and transmitting the processed image data to the graphic analysis unit;

S303, in the graphic analysis unit, the received image data is restored to relative spatial position according to its corresponding coordinate data; the restored image data is stitched by translation through difference processing, the repeated image data in adjacent pictures are compared and fused, and the pixel points in the overlapped area of the image are calculated by data dimensionality reduction, and the calculation formula is: ; where /> is the pixel coordinate in the image/> The pixel value after dimension reduction, is the pixel coordinate in the image/> Compare the pixel values of two adjacent images after fusion and coverage, /> is the pixel coordinate in the image/> Compare the pixel values of two adjacent images before fusion and coverage;

S304, by performing image data splicing and dimensionality reduction operations on all collected images, the collected multi-image data are merged into comprehensive microscopic image data of the slide observation target.

The S400 stores the slide positioning data, the slide detection path data corresponding to the slide detector and the scan data, and outputs the scan data:

S401, outputting the comprehensive microscopic image data of the slide observation target to a port;

S402, using the time point as an index, storing the current slide positioning data, the slide detection path data corresponding to the slide detector, the scanning data and the image analysis data in a diary period.

A slide detection and positioning system based on digital application, the system comprises a positioning data acquisition module, a detection and control center, a slide detection data analysis module and a multi-data storage and feedback module;

The positioning data acquisition module performs initial detection and positioning of the slide through the positioning beam source of the slide detection table device, and transmits the positioning data to the inspection and control unit in real time through the sensor equipment; the inspection and control center performs spatial evaluation of the placement matrix of the current slide according to the initial positioning data of the slide, analyzes the degree of offset of the initial position, and performs adaptive correction and regulation of the slide according to the analysis result; the slide detection data analysis module performs data scanning and extraction on the corrected slide, and dynamically plans and adjusts the data extraction scanning path of the slide detector and performs difference processing on the scanning data through the slide positioning correction data combined with the slide scanning data; the multi-data storage feedback module stores the slide positioning data, the slide detection path data corresponding to the slide detector and the scanning data, and outputs the scanning data.

The positioning data acquisition module includes a glass slide positioning data acquisition unit and a data transmission unit; the glass slide positioning data acquisition unit pre-installs a positioning beam source on a glass slide detection platform, and the glass slide detection platform is composed of a glass slide carrying table and a microscope detector; wherein the positioning beam source is installed on both the glass slide carrying table and the microscope detector respectively; the positioning beam source emits a group of uniform weak laser beams for positioning detection; in the two-dimensional plane of the glass slide carrying table, any horizontal side and any adjacent vertical side are installed with the positioning beam source; the initial position data of the glass slide is detected and collected by translating the positioning beam source on the horizontal side and its adjacent vertical side; with the scanning center point of the microscope detector as the correction point, by using the correction point as a reference point, the positioning beam source is installed around the scanning lens of the microscope detector, and the scanning position and orientation of the microscope detector on the glass slide are positioned, detected and collected; the data transmission unit transmits the collected data to the inspection and control center in real time through the sensing device; the sensing device is an IoT sensor.

The inspection and control center includes a spatial array evaluation unit, an initial position offset analysis unit and an adaptive position correction control unit; the spatial array evaluation unit uses the positioning beam source installed on the slide carrier to determine the side edge of the slide, and uses the vertical angle relationship between the two positioning beam sources to digitally determine the plane position of the slide; the slide carrier is constructed into a spatial cloud coordinate system through projection, and the two positioning beams of the slide carrier are used as the dynamic coordinate base axis, and the initial position of the current slide and the offset triangle constructed by the two positioning beams are obtained by translating the positioning beam from the starting point to the slide; the starting point is any end point on the side of the positioning beam source installed on the slide carrier; the offset angle of the initial position of the current slide is calculated through the offset triangle; the initial position offset angle of the current slide on the plane of the slide carrier is evaluated; the initial position offset analysis unit uses the positioning beam source installed around the scanning lens of the microscope detector to emit the positioning beam from the slide The method comprises the following steps: positioning the light beam, obtaining the coordinates of the positioning point of the positioning light beam on the plane of the glass slide carrier, determining the spatial dislocation of the coordinates, judging whether to perform a correction operation on the current position of the microscope detector, and judging whether the current observation point of the microscope detector exists in the glass slide position matrix; the adaptive correction control unit judges the correction demand for the initial position of the glass slide and the observation microscope according to the evaluation results of the above-mentioned spatial matrix and the initial position offset analysis results, and performs corresponding correction operations according to the judgment results; the initial offset position of the glass slide is corrected by a mechanical clamping and pushing device installed on the side of the glass slide carrier; the mechanical clamping and pushing device is folded and stored at the edge of the glass slide carrier, and when the slide position is corrected, it is released and its terminal clamping arm is used to fix and adjust the offset angle of the glass slide; the observation point of the glass slide is adjusted by moving the position of the observation microscope of the microscope detector; the top of the observation microscope of the microscope detector is connected with a connecting rod that supports extension and steering.

The slide detection data analysis module includes a dynamic scanning path planning unit and a graphic analysis unit; after the dynamic scanning path planning unit completes the alignment of the slide and the observation microscope, the observation microscope is used to scan and extract data from the slide; the coordinates of the current observation point and the coordinate matrix of the corresponding slide observation target are obtained in the coordinate plane of the slide carrier; by judging whether the coordinates of the current observation point exist in the coordinate matrix of the slide observation target; if so, the coordinates of the current observation point are used as the starting point of the microscope data scan, and the coordinate matrix of the current observation target is fully scanned by the adjacency traversal method; if not, the distance between the current observation point and the observation target is calculated; the minimum translation distance is taken, and the current observation point is translated to the coordinate matrix of the observation target, and the coordinates of the current observation point are used as the starting point of the microscope data scan, and the coordinate matrix of the current observation target is fully scanned by the adjacency traversal method; the adjacency traversal method The method is to take the coordinates of the current point as the starting point, analyze and calculate the adjacent coordinate points, and generate a moving path that traverses all coordinate points in the target area without duplication; use a microscope to fully traverse the coordinate matrix of the slide observation target, and extract the observation target image data at each coordinate point; mark the extracted image data according to the corresponding observation target coordinates, and transmit the processed image data to the graphic analysis unit; the graphic analysis unit restores the relative spatial position of the received image data according to its corresponding coordinate data; through difference processing, the restored image data is translated and spliced, the repeated image data in the adjacent pictures are compared and fused and covered, and the pixel points in the overlapped coverage area of the image are calculated for data dimensionality reduction; by performing image data splicing and dimensionality reduction operations on the collected images, the collected multiple image data are fused into comprehensive microscopic image data of the slide observation target.

The multi-data storage feedback module includes a target microscopic image data output unit and a full analysis data diary storage unit; the target microscopic image data output unit outputs the comprehensive microscopic image data of the slide observation target through a port; the full analysis data diary storage unit uses the time point as an index to store the current slide positioning data, the slide detection path data corresponding to the slide detector, the scanning data and the image analysis data in a diary period.

In the examples:

There is an automatic glass slide detector for glass slide data detection. The relevant staff places a glass slide carrying an observation target on a glass slide carrier, and then uses the positioning beam source installed on the glass slide carrier to determine the side edge of the glass slide, and uses the vertical angle relationship between the two positioning beam sources to digitally determine the plane position of the glass slide; the glass slide carrier constructs a spatial cloud coordinate system through projection, and uses the two positioning beams of the glass slide carrier as the dynamic coordinate base axis. The positioning beam is translated from the starting point to the glass slide to approximate the current initial position of the glass slide and the offset triangle constructed by the two positioning beams; the starting point is any end point on the side of the positioning beam source installed on the glass slide carrier; the offset angle of the current initial position of the glass slide is calculated by the offset triangle, and the calculation formula is: ; The calculated result of the offset angle of the current glass slide is 5 degrees; By evaluating the initial position offset angle of the current glass slide on the glass slide carrier plane, the current evaluation result is/> ; where /> If the position of the slide is 10 degrees, the automatic position correction system of the slide carrier will perform adaptive position correction on the current slide offset; the initial offset position of the slide will be corrected by the mechanical clamping and pushing device installed on the side of the slide carrier; the slide will be fixed and the offset angle will be adjusted by the terminal clamping arm;

The positioning beam source installed around the scanning lens of the microscope detector emits a positioning beam to the slide, and obtains the positioning point coordinates of the positioning beam on the plane of the slide carrier. , by determining the spatial dislocation of the coordinates, it is determined whether to perform a correction operation on the current microscope detector position. The judgment method is whether the current microscope detector observation point a exists in the slide position matrix; the current judgment result is/> , then the current microscope detector observation point exists in the slide position matrix, and there is no need to perform position correction operation on the microscope detector;

After the slide and the observation microscope are aligned, the slide is scanned and data is extracted using the observation microscope; the coordinates of the current observation point are obtained in the coordinate plane of the slide carrier. and the coordinate matrix corresponding to the slide observation target/> ; By judging whether the coordinates of the current observation point exist in the coordinate matrix of the target observed on the slide; if the current judgment result is yes, the coordinates of the current observation point are used as the starting point of the microscope data scan, and the coordinate matrix of the current observation target is fully scanned by the adjacency traversal method; the coordinate matrix of the target observed on the slide is fully traversed by the microscope, and the image data of the observed target is extracted at each coordinate point; the extracted image data is marked according to the corresponding coordinates of the observed target; the processed data is restored to relative spatial position, and the restored image data is translated and spliced through difference processing, the repeated image data in adjacent pictures are compared, fused and covered, and the pixel points in the overlapped area of the image are calculated for data dimensionality reduction. The calculation formula is/> ; By performing image data splicing and dimensionality reduction operations on the collected images, the collected multi-image data are fused into comprehensive microscopic image data of the slide observation target; the comprehensive microscopic image data of the slide observation target is output through a port, and the current slide positioning data, the slide detection path data corresponding to the slide detector, the scanning data and the image analysis data are stored in a diary periodicity with the time point as the index.

It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims (10)

1. A slide detection and positioning method based on digital application, characterized in that the method comprises the following steps: S100, installing a positioning beam source on a glass slide detection table, performing initial detection and positioning of the glass slide by the positioning beam source, and transmitting the positioning data to the inspection and control unit in real time by a sensor device; S200, the inspection and control unit performs a spatial evaluation on the placement array of the current glass slide according to the initial positioning data of the glass slide, analyzes the degree of deviation of the initial position, and performs adaptive position correction control on the glass slide according to the analysis result; S300, scanning and extracting data from the corrected slide, dynamically planning and adjusting the data extraction scanning path of the slide detector through the slide positioning correction data combined with the slide scanning data, and performing difference processing on the scanning data; S400, storing the slide positioning data, the slide detection path data corresponding to the slide detector and the scan data, and outputting the scan data. 2. A method for detecting and positioning a glass slide based on digital application according to claim 1, characterized in that: said S100 is provided with a positioning beam source on the glass slide detection table, performs initial detection and positioning of the glass slide by the positioning beam source, and transmits the positioning data to the inspection and control unit in real time by the sensor device: S101, pre-installing a positioning beam source on a glass slide detection platform, wherein the glass slide detection platform is composed of a glass slide carrying table and a microscope detector; wherein the positioning beam source is installed on both the glass slide carrying table and the microscope detector respectively; the positioning beam source emits a group of uniform weak laser beams for positioning detection; S102. In the two-dimensional plane of the glass slide supporting table, randomly select a horizontal side and any adjacent vertical side to position the light beam source; detect the initial position data of the glass slide by translating the positioning light beam source on the horizontal side and the adjacent vertical side, collect the detection data and transmit it to the inspection and control center in real time through the sensor equipment; take the scanning center point of the microscope detector as the correction point, use the correction point as a reference point, position the light beam source around the scanning lens of the microscope detector, perform positioning detection on the scanning position of the microscope detector on the glass slide, collect the detection data and transmit it to the inspection and control center in real time through the sensor equipment; the sensor equipment is an IoT sensor. 3. A method for detecting and positioning a glass slide based on digital application according to claim 2, characterized in that: the S200 inspection and control unit performs spatial evaluation on the placement matrix of the current glass slide according to the initial positioning data of the glass slide, analyzes the degree of deviation of the initial position, and performs adaptive position correction and control on the glass slide according to the analysis results as follows: S201, the inspection and control center receives the initial positioning data of the glass slide, and evaluates the spatial position of the placement matrix of the glass slide according to the initial positioning data of the glass slide, and the evaluation steps are as follows: S201-1, using the positioning beam source installed on the slide carrier to determine the side edge of the slide, and using the vertical angle relationship between the two positioning beam sources to digitally determine the plane position of the slide; S201-2, constructing a spatial cloud coordinate system for the glass slide carrier through projection, taking the two positioning beams of the glass slide carrier as the dynamic coordinate base axes, and obtaining the offset triangle between the initial position of the current glass slide and the two positioning beams by translating the positioning beams from the starting point to the glass slide; the starting point is any end point on the side of the positioning beam source of the device on the glass slide carrier; S201-3, calculating the offset angle of the initial position of the current slide by offsetting the triangle, and the calculation formula is: ; where /> is the offset angle, /> is the length of the hypotenuse of the offset triangle, /> is the length of the vertical side adjacent to the offset angle; S201-4, by evaluating the initial position deviation angle of the current slide on the slide carrier plane, if , the slides are manually repositioned and the initial position is re-analyzed; if /> , then the slide carrier automatic correction system will perform adaptive correction operation on the current slide offset; if /> , then the initial position of the slide does not need to be corrected; where /> Preset offset angles for the system; S202, obtaining the coordinates of the positioning point of the positioning beam on the plane of the glass slide carrier by emitting a positioning beam to the glass slide through a positioning beam source installed around the scanning lens of the microscope detector , by determining the spatial dislocation of the coordinates, it is determined whether to perform a correction operation on the current microscope detector position. The judgment method is whether the current microscope detector observation point a exists in the slide position matrix; if /> , then the current microscope detector observation point exists in the slide position matrix, and there is no need to perform position correction on the microscope detector; if/> , then the current microscope detector observation point does not exist in the slide position matrix, and the microscope detector is corrected; where the coordinates of observation point a are / > or or/> , the slide position matrix A is/> ; where i is the distance from the positioning beam source around the scanning lens of the microscope detector to the center of the scanning lens, /> 、/> , and/> are the coordinates of the end points of the edge of the slide respectively; S203, correcting the initial offset position of the glass slide by means of a mechanical clamping and pushing device installed on the side of the glass slide carrier; the mechanical clamping and pushing device is folded and stored at the edge of the glass slide carrier, and when the position of the glass slide is corrected, it is released and the glass slide is fixed and the offset angle is adjusted by using its terminal clamping arm; the observation point of the glass slide is adjusted by moving the position of the observation microscope of the microscope detector; the top of the observation microscope of the microscope detector is connected to a connecting rod that supports extension and steering. 4. A method for detecting and positioning a glass slide based on digital application according to claim 3, characterized in that: said S300 performs data scanning and extraction on the corrected glass slide, and dynamically plans and adjusts the data extraction scanning path of the glass slide detector by combining the glass slide positioning correction data with the glass slide scanning data, and performs difference processing on the scanning data as follows: S301, after the slide and the observation microscope are aligned, the slide is scanned and data is extracted using the observation microscope; the coordinates of the current observation point are obtained in the coordinate plane of the slide carrier and the coordinate matrix corresponding to the slide observation target/> ; By judging whether the coordinates of the current observation point exist in the coordinate matrix of the target observed on the slide; if so, the coordinates of the current observation point are used as the starting point of the microscope data scan, and the coordinate matrix of the current observation target is fully scanned using the adjacency traversal method; if not, the distance between the current observation point and the observation target is calculated, and the calculation formula is/> ; Where v is the distance between the current observation point and the observation target; take/> The minimum translation distance is used to translate the current observation point to the coordinate matrix of the observation target. The coordinates of the current observation point are used as the starting point of the microscope data scanning, and the adjacent traversal method is used to fully scan the coordinate matrix of the current observation target. The adjacent traversal method is to use the coordinates of the current point as the starting point, analyze and calculate the adjacent coordinate points, and generate a movement path that traverses all coordinate points in the target area without duplication; S302, traversing the coordinate matrix of the observation target on the slide through the microscope, and extracting the observation target image data at each coordinate point; marking the extracted image data according to the corresponding observation target coordinates, and transmitting the processed image data to the graphic analysis unit; S303, in the graphic analysis unit, the received image data is restored to relative spatial position according to its corresponding coordinate data; the restored image data is stitched by translation through difference processing, the repeated image data in adjacent pictures are compared and fused, and the pixel points in the overlapped area of the image are calculated by data dimensionality reduction, and the calculation formula is: ; where /> is the pixel coordinate in the image/> The pixel value after dimension reduction, is the pixel coordinate in the image/> Compare the pixel values of two adjacent images after fusion and coverage, /> is the pixel coordinate in the image/> Compare the pixel values of two adjacent images before fusion and coverage; S304, by performing image data splicing and dimensionality reduction operations on all collected images, the collected multi-image data are merged into comprehensive microscopic image data of the slide observation target. 5. A method for detecting and positioning a glass slide based on digital application according to claim 4, characterized in that: said S400 stores the glass slide positioning data, the glass slide detection path data corresponding to the glass slide detector and the scan data, and outputs the scan data: S401, outputting the comprehensive microscopic image data of the slide observation target to a port; S402, using the time point as an index, storing the current slide positioning data, the slide detection path data corresponding to the slide detector, the scanning data and the image analysis data in a diary period. 6. A slide detection and positioning system based on digital application, characterized in that: the system includes a positioning data acquisition module, a detection and control center, a slide detection data analysis module and a multi-data storage and feedback module; The positioning data acquisition module performs initial detection and positioning of the slide through the positioning beam source of the slide detection table device, and transmits the positioning data to the inspection and control unit in real time through the sensor equipment; the inspection and control center performs spatial evaluation of the placement matrix of the current slide according to the initial positioning data of the slide, analyzes the degree of offset of the initial position, and performs adaptive correction and regulation of the slide according to the analysis result; the slide detection data analysis module performs data scanning and extraction on the corrected slide, and dynamically plans and adjusts the data extraction scanning path of the slide detector and performs difference processing on the scanning data through the slide positioning correction data combined with the slide scanning data; the multi-data storage feedback module stores the slide positioning data, the slide detection path data corresponding to the slide detector and the scanning data, and outputs the scanning data. 7. A slide detection and positioning system based on digital application according to claim 6, characterized in that: the positioning data acquisition module includes a slide positioning data acquisition unit and a data transmission unit; the slide positioning data acquisition unit pre-installs a positioning beam source on a slide detection platform, and the slide detection platform is composed of a slide bearing table and a microscope detector; wherein the positioning beam source is installed on the slide bearing table and the microscope detector respectively; the positioning beam source emits a group of uniform weak laser beams for positioning detection; in the two-dimensional plane of the slide bearing table, any horizontal side and any adjacent vertical side are installed with the positioning beam source; the initial position data of the slide is detected and collected by translating the positioning beam source on the horizontal side and the adjacent vertical side; the scanning center point of the microscope detector is taken as the correction point, and the positioning beam source is installed around the scanning lens of the microscope detector by taking the correction point as the reference point, so as to perform positioning detection and collection on the scanning position and orientation of the microscope detector on the slide; the data transmission unit transmits the collected data to the inspection and control center in real time through the sensor device; the sensor device is an IoT sensor. 8. A glass slide detection and positioning system based on digital application according to claim 7, characterized in that: the inspection and control center includes a spatial array evaluation unit, an initial position offset analysis unit and an adaptive position correction control unit; the spatial array evaluation unit uses the positioning beam source installed on the glass slide carrier to determine the side edge of the glass slide, and uses the vertical angle relationship between the two positioning beam sources to digitally determine the plane position of the glass slide; the glass slide carrier is constructed into a spatial cloud coordinate system through projection, and the two positioning beams of the glass slide carrier are used as the dynamic coordinate base axis, and the current initial position of the glass slide and the offset triangle constructed by the two positioning beams are obtained by translating the positioning beam from the starting point to the glass slide; the starting point is any end point of the side of the positioning beam source installed on the glass slide carrier; the offset angle of the current initial position of the glass slide is calculated through the offset triangle; the initial position offset angle of the current glass slide on the plane of the glass slide carrier is evaluated; the initial position offset analysis unit is installed on the scanning device of the microscope detector The positioning beam source around the scanning lens emits a positioning beam to the glass slide, obtains the coordinates of the positioning point of the positioning beam on the plane of the glass slide carrier, and determines the spatial dislocation of the coordinates to determine whether to perform a correction operation on the current microscope detector position, and the judgment method is whether the current microscope detector observation point exists in the glass slide position matrix; the adaptive correction control unit judges the correction demand for the initial position of the glass slide and the observation microscope according to the evaluation results of the above-mentioned spatial matrix and the initial position offset analysis results, and performs corresponding correction operations according to the judgment results; the initial offset position of the glass slide is corrected by a mechanical clamping and pushing device installed on the side of the glass slide carrier; the mechanical clamping and pushing device is folded and stored at the edge of the glass slide carrier, and when the slide position is corrected, it is released and its terminal clamping arm is used to fix and adjust the offset angle of the glass slide; the observation point of the slide is adjusted by moving the position of the observation microscope of the microscope detector; the top of the observation microscope of the microscope detector is connected to a connecting rod that supports extension and steering. 9. A slide detection and positioning system based on digital applications according to claim 8, characterized in that: the slide detection data analysis module includes a dynamic scanning path planning unit and a graphic analysis unit; after the dynamic scanning path planning unit completes the correction of the slide and the observation microscope, the observation microscope is used to scan and extract data from the slide; the coordinates of the current observation point and the coordinate matrix of the corresponding slide observation target are obtained in the coordinate plane of the slide carrier; by judging whether the coordinates of the current observation point exist in the coordinate matrix of the slide observation target; if so, the current observation point coordinates are used as the starting point of the microscope data scan, and the coordinate matrix of the current observation target is fully scanned by the adjacency traversal method; if not, the distance between the current observation point and the observation target is calculated; the minimum translation distance is taken, and the current observation point is translated to the coordinate matrix of the observation target, and the current observation point coordinates are used as the starting point of the microscope data scan, and the adjacency traversal method is used to fully scan the coordinate matrix of the current observation target. The coordinate matrix of the target is fully scanned; the adjacency traversal method takes the coordinate of the current point as the starting point, and generates a movement path that traverses all coordinate points in the target area without duplication and fully by analyzing and calculating the adjacent coordinate points; the coordinate matrix of the target observed on the slide is fully traversed through a microscope, and the image data of the observed target is extracted at each coordinate point; the extracted image data is marked according to the corresponding observed target coordinates, and the processed image data is transmitted to the graphic analysis unit; the graphic analysis unit restores the relative spatial position of the received image data according to its corresponding coordinate data; the restored image data is translated and spliced through difference processing, the repeated image data in the adjacent pictures are compared and fused and covered, and the pixel points in the overlapped coverage area of the image are calculated for data dimensionality reduction; by performing image data splicing and dimensionality reduction operations on the collected images, the collected multiple image data are fused into comprehensive microscopic image data of the target observed on the slide. 10. A slide detection and positioning system based on digital applications according to claim 9, characterized in that: the multi-data storage feedback module includes a target microscopic image data output unit and a full analysis data diary storage unit; the target microscopic image data output unit outputs the comprehensive microscopic image data of the slide observation target through a port; the full analysis data diary storage unit uses the time point as an index to store the current slide positioning data, the slide detection path data corresponding to the slide detector, the scanning data and the image analysis data in a diary period.