CN117606885B - Intelligent pathological imaging system and method - Google Patents
Intelligent pathological imaging system and method Download PDFInfo
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- CN117606885B CN117606885B CN202410095591.9A CN202410095591A CN117606885B CN 117606885 B CN117606885 B CN 117606885B CN 202410095591 A CN202410095591 A CN 202410095591A CN 117606885 B CN117606885 B CN 117606885B
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- 238000000034 method Methods 0.000 title claims abstract description 42
- 230000001575 pathological effect Effects 0.000 title claims abstract description 12
- 238000003384 imaging method Methods 0.000 title claims description 9
- 238000002360 preparation method Methods 0.000 claims abstract description 9
- 239000011521 glass Substances 0.000 claims description 172
- 238000004062 sedimentation Methods 0.000 claims description 136
- 230000007246 mechanism Effects 0.000 claims description 102
- 238000007789 sealing Methods 0.000 claims description 74
- 238000001914 filtration Methods 0.000 claims description 50
- 238000004043 dyeing Methods 0.000 claims description 40
- 238000003860 storage Methods 0.000 claims description 38
- 238000012546 transfer Methods 0.000 claims description 38
- 239000007788 liquid Substances 0.000 claims description 25
- 230000007170 pathology Effects 0.000 claims description 18
- 230000000712 assembly Effects 0.000 claims description 16
- 238000000429 assembly Methods 0.000 claims description 16
- 238000004891 communication Methods 0.000 claims description 10
- 230000009471 action Effects 0.000 claims description 8
- 238000007599 discharging Methods 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 8
- 238000013461 design Methods 0.000 claims description 7
- 230000005540 biological transmission Effects 0.000 claims description 6
- 230000000149 penetrating effect Effects 0.000 claims description 4
- 230000001086 cytosolic effect Effects 0.000 claims 1
- 230000008569 process Effects 0.000 abstract description 21
- 230000002195 synergetic effect Effects 0.000 abstract description 4
- 238000011084 recovery Methods 0.000 description 27
- 230000000694 effects Effects 0.000 description 15
- 230000000670 limiting effect Effects 0.000 description 11
- 238000010586 diagram Methods 0.000 description 9
- 238000009434 installation Methods 0.000 description 8
- 238000004140 cleaning Methods 0.000 description 5
- 230000001678 irradiating effect Effects 0.000 description 4
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- 238000010276 construction Methods 0.000 description 2
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- 230000010354 integration Effects 0.000 description 2
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- 238000012216 screening Methods 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 230000001174 ascending effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002457 bidirectional effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
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Abstract
The invention discloses the technical field of cell slide preparation equipment, in particular to an intelligent pathological slide preparation system and an intelligent pathological slide preparation method. The method is implemented based on the system. The invention has the technical characteristics of simple and reasonable structure, high tabletting efficiency and better process connection and synergistic effect.
Description
Technical Field
The invention relates to the technical field of cell tabletting equipment, in particular to an intelligent pathological tabletting system.
Background
The pathological flaking technique is a technique in which exfoliated cells are preserved in a liquid, and the cells are uniformly dispersed and attached to a glass slide by a special device to make a smear.
The cell pelleter is an automatic pelleter for finishing the smear of the exfoliated cells by using a cytology detection technology, can obviously improve the quality of pathological cytology smear, and is an ideal screening tool for cervical lesions. In the prior art, most of pelleters are either semi-automatic or combined, and a small amount of pelleters are fully automatic; the semi-automatic type manual matching part operation is required, the efficiency is low, mistakes are easy to occur, and the problems of human errors and sample pollution exist; the combined type device is formed by arranging a plurality of devices with different functions according to the sequence of the film-making working procedures, and the device can basically separate from the operation of people, so that human errors can be reduced, but the devices are not tightly connected, the efficiency is low, the whole device occupies large area and has high cost, and manual screening and assistance are still needed in most cases; finally, although the full-automatic equipment in the prior art can realize the flaking process in a full-automatic way, manual participation is not needed, the fault tolerance rate is low, the equipment structure is complex, each procedure is completed by a plurality of modularized structures, part of flaking procedures are still completed by separate equipment and then are carried out by flaking equipment, the control and collaboration difficulties are large, the manufacturing cost is high, and the large-scale industrial application is not facilitated.
In view of the above, the present invention aims to provide a tabletting apparatus, which reduces the cost and the structural complexity of the tabletting apparatus, and improves the synergistic effect and tabletting efficiency of the apparatus.
Disclosure of Invention
The invention aims to provide an intelligent pathological imaging system which has the technical characteristics of simple and reasonable structure, high imaging efficiency, and better working procedure connection and synergistic effect.
The aim of the invention is mainly realized by the following technical scheme: an intelligent pathological imaging system comprises an equipment main body, wherein a sample bottle code scanning and uncovering system, a liquid transferring system, a cell liquid filtering system, a glass slide feeding and coding system, a multi-station sedimentation dyeing system, a glass slide ultraviolet sealing system and a glass slide box storage assembly system are arranged on the equipment main body; the sample bottle code scanning and uncovering system is used for transferring the sample bottles to the liquid transferring system and executing code scanning and uncovering operation on the sample bottles; the pipetting system is used for transferring samples in the sample bottles to the cell liquid filtering system for filtering, and transferring the filtered samples to the glass slide feeding and coding system; the cell fluid filtering system is used for filtering a sample to extract effective cell fluid; the slide glass feeding and coding system is used for providing slide glass to bear samples, coding the slide glass and sequentially conveying the slide glass to the multi-station sedimentation and dyeing system and the slide glass ultraviolet sealing system; the multi-station sedimentation dyeing system is used for carrying out sedimentation dyeing on a sample on a glass slide; the slide ultraviolet sealing system is used for performing ultraviolet sealing on the sample on the sedimentation-dyed slide; the slide box storage assembly system is used for collecting and storing the slide glass subjected to ultraviolet sealing.
Based on the technical scheme, the sample bottle code scanning and cover opening system comprises a sample bottle positioning and conveying device, a sample bottle transferring device and a scanner; the sample bottle positioning and conveying device comprises a first conveying belt and a first positioning plate fixed above the first conveying belt, wherein a plurality of first through grooves for positioning sample bottles are formed in the first positioning plate, and the first through grooves are formed in the conveying direction of the first conveying belt; the sample bottle transferring device comprises a first two-axis moving mechanism and a sample bottle clamping jaw connected with the first two-axis moving mechanism, wherein the sample bottle clamping jaw is used for grabbing a sample bottle and transferring the sample bottle to a pipetting system based on the first two-axis moving mechanism; the scanner is arranged in the moving track of the first two-axis moving mechanism, and is used for scanning the information code outside the sample bottle after the sample bottle is transported to the pipetting system.
Based on the technical scheme, the pipetting system comprises a sample bottle bearing seat, a TIP pipette bearing seat, a first TIP pipetting system and a second TIP pipetting system; the sample bottle bearing seat comprises a pneumatic rotary clamping jaw arranged on one side of the TIP pipette bearing seat, and the clamping jaw end of the pneumatic rotary clamping jaw is used for grasping a sample bottle and driving the sample bottle to rotate under the action of the rotating end of the pneumatic rotary clamping jaw; the TIP pipette bearing seat comprises a supporting frame, wherein a plurality of mounting grooves are formed in the supporting frame and are used for mounting a TIP pipette tray, and a plurality of TIP pipette placing holes are formed in the TIP pipette tray; the first TIP pipetting system is used for adsorbing the sample in the sample bottle to the cell sap filtering system; the second TIP pipetting system is used for transferring the sample filtered by the cell liquid filtering system to a glass slide supply coding system; the TIP pipette tray is disposed within a track range of pipette TIPs of the first TIP pipetting system and the second TIP pipetting system.
Based on the technical scheme, the TIP pipetting system further comprises a TIP pipette temporary storage rack, wherein a plurality of hanging holes for hanging the TIP pipettes are formed in the TIP pipette temporary storage rack; after the first TIP pipetting system collects the sample, the TIP pipette can be temporarily stored on the hanging hole for the second TIP pipetting system to use when the same sample is transferred.
Based on the technical scheme, the cell sap filtering system comprises a first bracket, a rotating shaft is arranged on the first bracket, at least one clamping jaw assembly for bearing a filtering cup is fixed on the rotating shaft, and the filtering cup is used for containing samples transferred by a pipetting system; the rotating shaft is also connected with a first driving mechanism, and the first driving mechanism is used for driving the rotating shaft to rotate; the rotating shaft is also sleeved with a rotating frame, the rotating frame is connected with a second driving mechanism, and the second driving mechanism is used for driving the rotating frame to rotate relative to the rotating shaft; still be provided with flexible subassembly on the rotating turret, flexible end of flexible subassembly is connected with the closure board, the closure board is used for sealing filter bowl, still be provided with the pressurization pipeline on the closure board, the pressurization pipeline is provided with the multichannel and every way pressurization pipeline all runs through the closure board, every way the pressurization pipeline all corresponds alone behind the penetration of closure board one filter bowl.
Based on the technical scheme, the clamping jaw assemblies and the telescopic assemblies are driven by adopting pneumatic structures; the cell sap filtering system also comprises a pneumatic control system; the pneumatic control system comprises a communicated air source, a filter valve and a first three-way valve, wherein two outlets of the first three-way valve are respectively communicated with a control loop and a pressurizing loop; the control loop comprises a plurality of middle sealing electromagnetic valves which are communicated with the outlets of the first three-way valve, and the outlet of each middle sealing electromagnetic valve is independently communicated with a clamping jaw assembly or a telescopic assembly; the pressurizing loop comprises a first two-way valve, a precise pressure reducing valve and a plurality of second two-way valves which are communicated, wherein the first two-way valve is communicated with an outlet of the first three-way valve, the plurality of second two-way valves are communicated with an outlet of the precise pressure reducing valve, and outlet ends of the second two-way valves are independently communicated with one pressurizing pipeline.
Based on the technical scheme, the cell sap filtering system further comprises a filter cup transferring system; the filter cup transferring system comprises a filter cup positioning and conveying device and a filter cup transferring device; the filter cup positioning and conveying device comprises a second conveying belt and a second positioning plate fixed above the second conveying belt, wherein a plurality of second through grooves for positioning filter cups are formed in the second positioning plate, and the second through grooves are formed in the conveying direction of the second conveying belt; the filter cup transferring device comprises a second two-axis moving mechanism and a filter cup clamping jaw connected with the second two-axis moving mechanism, wherein the filter cup clamping jaw is used for grabbing a filter cup and transferring a sample filter cup to a clamping jaw assembly based on the second two-axis moving mechanism.
Based on the technical scheme, the slide glass supplying and coding system comprises a slide glass mounting system, a multi-station turntable system and a laser coding machine; the multi-station turntable system comprises a rotation driving mechanism and a turntable connected with the rotation driving mechanism, wherein a plurality of glass slide placing grooves are circumferentially distributed on the turntable at intervals, each glass slide placing groove is provided with a sedimentation cup clamping plate, and the sedimentation cup clamping plates are opposite to the glass slide placing grooves up and down and form a gap therebetween; the middle part of the sedimentation cup clamping plate vertically penetrates through a sedimentation cup mounting hole, and two first through-hole grooves are oppositely formed in the side wall of the sedimentation cup mounting hole; the slide glass mounting system comprises a second bracket, a slide glass feed box is mounted on the second bracket, a feed cavity for stacking slide glass is formed in the slide glass feed box, a discharge channel is horizontally formed in the lower end of the slide glass feed box, and the discharge channel is communicated with the feed cavity; the second bracket is also provided with a linear driving mechanism and a push plate connected with the moving end of the linear driving mechanism, and the push plate penetrates through the slide feeding box and can extend out through the outlet end of the discharging channel so as to push out the slide in the slide feeding box into the slide placing groove; the carousel is located every slide glass standing groove's below still runs through and is provided with the breach, the laser coding machine sets up in the carousel below, and its laser emission end pass through the breach with the slide glass is relative.
Based on the technical scheme, the push plate is a flat plate, the discharging channel is a square channel penetrating through the slide glass feeding box, and the heights and the widths of the flat plate and the square channel are the same as those of the slide glass.
Based on the above technical scheme, the exit end of discharge channel with the marginal interval setting of carousel, and interval distance is less than the length of slide glass, just the exit end of discharge channel with slide glass standing groove flushes the setting.
Based on the technical scheme, the slide glass supplying and coding system further comprises a sedimentation cup transferring system; the sedimentation cup transfer system comprises a chain plate conveying device and a sedimentation cup transfer device; the chain plate conveying device comprises a chain plate driving mechanism and a conveying chain plate assembly in transmission connection with the chain plate driving mechanism, wherein the chain plates of the conveying chain plate assembly are provided with sedimentation cup positioning grooves, a plurality of sedimentation cup positioning grooves are arranged at intervals along the length direction of the chain plate, and two second through-hole grooves are formed in the side wall of each sedimentation cup positioning groove relatively; the sedimentation cup transfer device comprises a third two-axis moving mechanism and a sedimentation cup rotating clamping jaw connected with the third two-axis moving mechanism, wherein the sedimentation cup rotating clamping jaw is used for grabbing a sedimentation cup, and the sample sedimentation cup is transported into a sedimentation cup mounting hole based on the third two-axis moving mechanism and is fixed in the sedimentation cup mounting hole through rotation.
Based on the technical scheme, the multi-station sedimentation dyeing system comprises a plurality of dyeing devices which are sequentially arranged along the circumferential direction of the turntable; the dyeing equipment comprises a lifting rotating assembly and a pump head assembly connected with the lifting rotating assembly, wherein the pump head assembly is used for conveying corresponding liquid agent into the sedimentation cup so as to dye and clean a sample, and sucking the liquid agent.
Based on the technical scheme, the slide ultraviolet sealing system comprises a base, wherein a slide chute is arranged on the base, and two ends of the slide chute are respectively arranged at intervals with a turntable and a slide box storage assembly system; the base is sequentially provided with a curing agent adding system and an ultraviolet lamp irradiation system along the upper part of the slide chute; the bottom of the slide chute is hollowed out to form a communication port, the base is also provided with a lifting module, a linear module connected to the lifting module and a slide push-pull plate connected to the linear module, and the slide push-pull plate can push a slide from the turntable to the slide chute and push the slide to the slide box storage assembly system along the slide chute under the action of the lifting module and the linear module.
Based on the technical scheme, the slide glass push-pull plate is provided with the baffle plates in an upward protruding mode along the two ends and the middle of the slide glass push-pull plate in the length direction, and a first limit groove and a second limit groove are formed between the three baffle plates.
Based on the technical scheme, the lengths of the first limit groove and the second limit groove are larger than or equal to the length of the glass slide; the width of the slide glass push-pull plate is smaller than the width of the communication port, and the width of the slide glass push-pull plate in the area where the first limiting groove is located is smaller than or equal to the width of a notch below the slide glass placing groove.
Based on the technical scheme, slide box storage assembly system includes lift base and sets up the slide box on the lift base, and the slide box is provided with an opening that is used for the slide glass to get into at least, be provided with the multirow draw-in groove along its direction of height symmetry in the slide box.
Compared with the prior art, the invention has the following beneficial effects:
1. the intelligent pathological tabletting system has the advantages of simple and reasonable overall structure, tight connection between each system and each working procedure, good synergistic effect, high overall tabletting efficiency, low fault tolerance, low cost and good application prospect.
2. The pipetting system can simultaneously realize pipetting procedures of samples in the sample bottle and samples in the filtering system, has a simple overall structure and high integration degree, and the first TIP pipetting system and the second TIP pipetting system can share the TIP pipette of the same sample, so that the waste of the TIP pipette is reduced, the system structure can be simplified, the control difficulty is reduced, and the implementation of the system are facilitated.
3. The filter system can realize the sealing or opening of the filter cup by utilizing the sealing plate, further can realize the state control of the filter cup by combining the first bracket, the rotating frame, the first driving mechanism, the rotating shaft and the second driving mechanism, can pressurize the filter cup by the pressurizing pipeline in the filtering process, can improve the filtering effect by combining the shaking effect of the integral structure, ensures that invalid cells of a sample can be filtered maximally and only valid cells are reserved, and improves the accuracy of the sample.
4. The slide glass feeding and coding system can realize feeding, sample adding, coding and multi-station sedimentation dyeing of a plurality of slide glass by utilizing the turntable structure, so that the slide glass preparation operation of a plurality of samples is better realized, the occupied area of equipment is reduced, the slide preparation process is simplified, and the slide preparation efficiency is improved.
5. According to the slide ultraviolet sealing system, the transfer requirements of different stations of a slide can be met through the slide push-pull plate, so that the slide can be kept in a horizontal state in the transfer process, further, samples on the slide can be ensured to be stably carried at corresponding positions, scattering or scattering of the samples is avoided, other equipment or procedures cannot be influenced in the process of executing the corresponding stations through the slide push-pull plate, independent slide operation is achieved, the slide directly enters a slide box storage assembly system for storage after solidification is finished, damage of the samples in the secondary transfer process can be avoided, the slide is directly sent into a slide chute in a horizontal and equal-height state by utilizing a multi-station turntable system before the slide enters the solidification, the stability of the slide can be further ensured, the position and the state of the slide ultraviolet sealing are further ensured by combining the limiting effect of the slide chute, and the ultraviolet sealing effect can be improved.
Meanwhile, the invention also provides an intelligent pathology sheeting method based on the intelligent pathology sheeting system, which comprises the following steps:
S1
the sample bottle code scanning and cover opening system transfers the sample bottle to a sample bottle bearing seat of the pipetting system, and performs code scanning and cover opening operation on the sample bottle, and the filter cup transferring system synchronously transfers the filter cup to the clamping jaw assembly for clamping;
S2
the first TIP pipetting system adsorbs samples in the sample bottles on the sample bottle bearing seat to the filter cup;
S3
the second driving mechanism drives the rotating frame to rotate relative to the rotating shaft, the sealing plate is rotated to a position opposite to the cup opening of the filter cup, the telescopic assembly starts to drive the sealing plate to move until the sealing cup opening is pressed, the pressurizing pipeline blows air into the filter cup to pressurize and keep a pressurizing state, the first driving mechanism is started to drive the rotating shaft to rotate after the pressurization pipeline blows air into the filter cup, the clamping jaw assembly and the rotating frame are driven to synchronously and circularly reciprocate, the first driving mechanism stops after the rotation design time, and the second driving mechanism is started to drive the rotating frame to rotate relative to the rotating shaft and stagger the filter cup;
S4
the glass slide mounting system conveys glass slides to a glass slide placing groove, the rotary table rotates to the position of the sedimentation cup transfer system, the sedimentation cup transfer system conveys sedimentation cups to the sedimentation cup mounting holes and clamps the sedimentation cups through the first through-hole groove, the rotary table rotates again to rotate the glass slides to the position of the second TIP liquid transfer system, the second TIP liquid transfer system adsorbs samples in the filter cups on the clamping jaw assemblies onto the glass slides on the rotary table, the rotary table continuously rotates and sequentially carries out coding at the position of the laser coding machine, the multi-station sedimentation dyeing system carries out sedimentation dyeing and then conveys the glass slides to the glass slide ultraviolet sealing system for sedimentation dyeing, and the rotary table continuously rotates to the glass slide ultraviolet sealing system after the sedimentation dyeing is completed;
S5
The slide glass push-pull plate extends to the slide glass sliding groove and extends below the slide glass placing groove, and synchronously rises to enable the slide glass to be located in a first limit groove of the slide glass push-pull plate, the slide glass push-pull plate moves back to hook the slide glass on the slide glass sliding groove by using a baffle plate at the outer end part and is located below a curing agent adding system, after the curing agent adding system adds the curing agent to a sample area on the slide glass, the slide glass push-pull plate moves downwards and synchronously translates a second limit groove to the lower part of the slide glass, then moves upwards to limit the slide glass to the second limit groove, after the slide glass push-pull plate translates to the lower part of an ultraviolet lamp irradiation system to carry out ultraviolet sealing piece, after the slide glass push-pull plate moves downwards and synchronously translates a baffle plate at the outer end part of the second limit groove to one side of the slide glass, and then moves upwards and translates, and pushes the slide glass to a slide box storage assembly system by using the baffle plate at the outer end part of the second limit groove;
S6
the slide box storage assembly system adjusts the height of the slide box so that any row of clamping grooves in the slide box are aligned with the slides, and the slide push-pull plate pushes the slides to enter the slide box from the opening of the slide box and to be clamped in the corresponding clamping grooves for storage, so that the slide preparation is completed.
The method has the characteristics of compact and simple integral process connection, improved sample filtering effect and tabletting efficiency, simple tabletting process, high efficiency and good effect, and is suitable for large-area popularization and application.
Drawings
The accompanying drawings, which are included to provide a further understanding of embodiments of the invention and are incorporated in and constitute a part of this application, illustrate embodiments of the invention. In the drawings:
FIG. 1 is a schematic diagram of a first configuration of an intelligent pathology sheeting system according to an embodiment;
FIG. 2 is a second schematic diagram of an embodiment of a system for intelligent pathology imaging;
FIG. 3 is a schematic diagram of a third configuration of an intelligent pathology sheeting system according to an embodiment;
FIG. 4 is a fourth schematic diagram of an embodiment of a system for intelligent pathology imaging;
FIG. 5 is a top view of an embodiment of a system for intelligent pathology imaging;
FIG. 6 is a schematic diagram of the sample bottle code scanning and uncapping system and the filter cup transfer system according to the embodiment;
FIG. 7 is a schematic view of a sample bottle carrier according to an embodiment, wherein a part of the structure is omitted;
fig. 8 is an enlarged view of the structure at E in fig. 1, in which A, B, C shows a first recovery cylinder a, a second recovery cylinder B, and a third recovery cylinder C, respectively;
FIG. 9 is a schematic diagram showing a first construction of a cell fluid filter system according to an embodiment, wherein the filter bowl is in a closed position;
FIG. 10 is a front view of a cell fluid filtration system according to an embodiment;
FIG. 11 is a schematic view showing a second construction of the cell sap filtration system of the embodiment, in which the filter cup is in an open state;
FIG. 12 is a schematic diagram of a pneumatic control system in an embodiment;
FIG. 13 is a schematic view showing a structure of a slide feeding and coding system according to an embodiment, in which D represents a fourth recovery cylinder D;
FIG. 14 is a schematic diagram of a multi-station sedimentation dyeing system in an embodiment;
FIG. 15 is a schematic view of the overall structure of a slide feed indexing system, a slide UV sealing system, and a slide cassette storage assembly system according to an embodiment;
fig. 16 is an enlarged view of the structure at F in fig. 15;
FIG. 17 is a partial block diagram of a slide UV sealing system;
FIG. 18 is a schematic view of a slide push-pull plate;
the reference numerals in the figures are respectively expressed as:
1. an apparatus main body; 2. a scanner; 3. a first conveyor belt; 4. a first positioning plate; 5. a first through groove; 6. a sample bottle; 7. a first two-axis moving mechanism; 8. sample bottle clamping jaws; 9. a sample bottle bearing seat; 10. TIP pipette carrier; 11. a first TIP pipetting system; 12. a second TIP pipetting system; 13. pneumatically rotating the clamping jaw; 14. a support frame; 15. TIP pipette tray; 16. pipette tip; 17. TIP pipette temporary storage rack; 18. a hanging hole; 19. a first bracket; 20. a rotating shaft; 21. a jaw assembly; 22. a first driving mechanism; 23. a rotating frame; 24. a second driving mechanism; 25. a closing plate; 26. a pressurizing pipeline; 27. a soft cushion; 28. a fixed wheel; 29. a driving wheel; 30. a guide cylinder; 31. a guide rod; 32. a gas source; 33. a filter valve; 34. a first three-way valve; 35. a control loop; 36. a pressurization circuit; 37. a middle seal electromagnetic valve; 38. a first two-way valve; 39. a precision pressure reducing valve; 40. a second two-way valve; 41. a second conveyor belt; 42. a second positioning plate; 43. a second through slot; 44. a second two-axis moving mechanism; 45. a clamping jaw of the filter cup; 46. a rotation driving mechanism; 47. a turntable; 48. a slide glass placing groove; 49. a settling cup clamping plate; 50. a settling cup mounting hole; 51. a first through slot; 52. a second bracket; 53. slide feed cassettes; 54. a feed chamber; 55. a linear driving mechanism; 56. a push plate; 57. a notch; 58. a link plate driving mechanism; 59. a conveying chain plate assembly; 60. a settling cup positioning groove; 61. a second vent slot; 62. a third two-axis moving mechanism; 63. the sedimentation cup rotates the clamping jaw; 64. a lifting rotating assembly; 65. a pump head assembly; 66. a base; 67. slide glass chute; 68. a curing agent addition system; 69. an ultraviolet lamp illumination system; 70. a communication port; 71. a lifting module; 72. a linear module; 73. slide glass push-pull plate; 74. a baffle; 75. a first limit groove; 76. the second limit groove; 77. lifting a base; 78. a glass slide box; 79. an opening; 80. a clamping groove.
Detailed Description
For the purpose of making apparent the objects, technical solutions and advantages of the present invention, the present invention will be further described in detail with reference to the following examples and the accompanying drawings, wherein the exemplary embodiments of the present invention and the descriptions thereof are for illustrating the present invention only and are not to be construed as limiting the present invention.
As shown in fig. 1-5, a first embodiment of the present invention provides an intelligent pathological film forming system, which comprises an apparatus main body 1, wherein a sample bottle code scanning and uncovering system, a pipetting system, a cell liquid filtering system, a glass slide feeding and coding system, a multi-station sedimentation dyeing system, a glass slide ultraviolet film sealing system and a glass slide box storage assembly system are arranged on the apparatus main body 1; the sample bottle code scanning and uncovering system is used for transferring the sample bottles to the liquid transferring system and executing code scanning and uncovering operation on the sample bottles; the pipetting system is used for transferring samples in the sample bottles to the cell liquid filtering system for filtering, and transferring the filtered samples to the glass slide feeding and coding system; the cell fluid filtering system is used for filtering a sample to extract effective cell fluid; the slide glass feeding and coding system is used for providing slide glass to bear samples, coding the slide glass and sequentially conveying the slide glass to the multi-station sedimentation and dyeing system and the slide glass ultraviolet sealing system; the multi-station sedimentation dyeing system is used for carrying out sedimentation dyeing on a sample on a glass slide; the slide ultraviolet sealing system is used for performing ultraviolet sealing on the sample on the sedimentation-dyed slide; the slide box storage assembly system is used for collecting and storing the slide glass subjected to ultraviolet sealing.
When implementing, the sample bottle is through the sample bottle sweep sign indicating number uncapping system after transferring, scanning and uncapping, carry out sample transfer by the pipetting system, cell liquid filtration system carries out sample filtration, the feed of slide glass is carried out to the rethread multistation subsides dyeing system, code printing and subside dyeing, finally carry out the ultraviolet package piece through slide glass ultraviolet package piece system and store by slide box storage assembly system is concentrated, overall structure links up closely, simple structure, the degree of integration is high, can effectively improve the film-making efficiency of sample, reduce the film-making process time and reduce the film-making degree of difficulty.
As shown in fig. 6, the sample bottle code scanning and cover opening system comprises a sample bottle positioning and conveying device, a sample bottle transferring device and a scanner 2; the sample bottle positioning and conveying device comprises a first conveying belt 3 and a first positioning plate 4 fixed above the first conveying belt 3, wherein a plurality of first through grooves 5 for positioning sample bottles 6 are formed in the first positioning plate 4, and the first through grooves 5 are formed in the conveying direction of the first conveying belt 3; the sample bottle transferring device comprises a first two-axis moving mechanism 7 and a sample bottle clamping jaw 8 connected with the first two-axis moving mechanism 7, wherein the sample bottle clamping jaw 8 is used for grabbing a sample bottle and transferring the sample bottle to a pipetting system based on the first two-axis moving mechanism 7; the scanner 2 is arranged in the moving track of the first two-axis moving mechanism 7, and after the sample bottle is transferred to the pipetting system, the scanner 2 is used for scanning the information code outside the sample bottle.
When implementing, the appearance bottle that encapsulates the sample is placed and is fixed a position in first logical groove 5, after first conveyer belt 3 starts, the appearance bottle translates along first logical groove 5 under first locating plate 4 effect, and then carry the sample to appointed snatch the position, first diaxon moving mechanism 7 can drive sample clamping jaw translation and vertical lift to snatch the position and snatch the sample, and carry the sample to pipetting system, can realize the transportation of sample, and in the transportation process or after accomplishing the transportation, scanner 2 can also scan the information code in the sample outside like two-dimensional code, bar code etc. obtain the patient information that the sample corresponds.
In specific application, the first through groove 5 can be provided with a plurality of sample bottles side by side so as to increase more sample bottle positioning and conveying positions. Further, the end portion of the first through groove 5 located in the conveying direction of the first conveying belt 3 is provided with a gear piece or a sensing piece, such as a gear block, a laser sensor and the like, and is used for detecting whether the sample bottle reaches the grabbing position or not, and further ensuring conveying of the sample bottle and conveying accuracy.
In a specific application, the scanner 2 can adopt an XN-1090HD type scanner manufactured by Dewa, so as to acquire different types of information codes.
In specific application, the first two-axis moving mechanism 7 can be used by adopting a conventional two-axis moving platform, an XY two-axis platform and the like, while the sample bottle clamping jaw 8 is mainly used for clamping a sample bottle and is convenient to move, and can be formed by adopting a mechanical arm, clamping jaws of various driving sources and the like in the prior art during implementation. Specifically, the sample bottle clamping jaw 8 of this embodiment mainly includes the drive end and the execution end of being connected with the drive end, and the execution end is the clamping jaw that two intervals set up, and the clamping jaw sets up to U type structure, and when the clamping jaw is driven to be close to under the drive end, the clamping jaw centre gripping is on the bottle lid of sample bottle, and then with sample bottle centre gripping, and because U type structural design, the clamping jaw relative both sides can carry out fine matching with the anti-skidding groove on the sample, and then the centre gripping is firm after the centre gripping, and can prevent that the sample bottle from rotating.
When the sample bottle code scanning and uncovering system is specifically applied, the sample bottle code scanning and uncovering system further comprises a first recovery cylinder A, a recovery box communicated with the first recovery cylinder A is further arranged in the equipment main body 1, and the first recovery cylinder A is located in the moving track of the first two-axis moving mechanism 7. Furthermore, after the sample bottle is transferred, the sample bottle clamping jaw 8 can discard the abandoned sample bottle clamping to the first recovery cylinder A and uniformly collect the sample bottle by the recovery box, so that the pollution caused by the leakage of the sample bottle is avoided, and the continuous process of the process is not influenced.
As shown in fig. 7 and 8, the pipetting system comprises a sample bottle carrying seat 9, a TIP pipette carrying seat 10, a first TIP pipetting system 11 and a second TIP pipetting system 12; the sample bottle bearing seat 9 comprises a pneumatic rotary clamping jaw 13 arranged on one side of the TIP pipette bearing seat 10, and the clamping jaw end of the pneumatic rotary clamping jaw 13 is used for clamping a sample bottle and driving the sample bottle to rotate under the action of the rotating end of the pneumatic rotary clamping jaw 13; the TIP pipette bearing seat 10 comprises a supporting frame 14, wherein a plurality of mounting grooves are formed in the supporting frame and are used for mounting a TIP pipette tray 15, and the TIP pipette tray 15 is provided with a plurality of TIP pipette placing holes; the first TIP pipetting system 11 is used for adsorbing a sample in a sample bottle to a cell sap filtering system; the second TIP pipetting system 12 is used for transferring the sample filtered by the cell liquid filtering system to a slide glass supply coding system; the TIP pipette tray 15 is disposed within the track of the pipette TIPs 16 of the first TIP pipetting system 11 and the second TIP pipetting system 12.
When the sample bottle is placed on the sample bottle carrying seat 9, the pneumatic rotary clamping jaw 13 clamps the sample bottle and then rotates, the clamping jaw of the sample bottle code scanning and cover opening system can clamp the sample bottle on a bottle cover of the sample bottle, then the sample bottle is opened by rotating a bottle body of the sample bottle and not rotating the bottle cover, a plurality of TIP pipettes can be pre-installed in a TIP pipette placing hole on the TIP pipette tray 15, after the cover opening of the sample bottle is completed, the first TIP pipetting system 11 can be moved to the corresponding TIP pipette for installing the TIP pipette, then the sample bottle is moved to the sample bottle carrying seat 9 for adsorbing samples in the sample bottle, the sample bottle is transferred to the filtering system, the second TIP pipetting system 12 can be moved to the TIP pipette on the TIP pipette tray 15, and the filtered sample bottle is transferred to the glass slide feeding and frame opening system after the sample bottle is installed, and the sample bottle pipetting procedure is completed.
In specific applications, the first TIP pipetting system 11 and the second TIP pipetting system 12 may be used by using a tablet-making and washing system in the prior art, so that the structure and the working principle thereof are not specifically described in this embodiment, and similarly, the TIP 16 of the pipette may be used to adsorb and mount the TIP pipette in a pneumatic manner in the prior art, and the structure and the working principle thereof are not described in any way.
When the recovery cylinder a is disposed on the apparatus main body 1, the pneumatic rotary clamping jaw 13 can reversely rotate after transferring the sample bottles, and the clamping jaw of the sample bottle code scanning and cover opening system can clamp the bottle cap of the sample bottle to move downwards, so as to cover the discarded sample bottles for centralized discarding and recovery.
In specific application, the TIP pipetting system further comprises a TIP pipette temporary storage rack 17, wherein a plurality of hanging holes 18 for hanging the TIP pipettes are formed in the TIP pipette temporary storage rack 17; the first TIP pipetting system 11 may temporarily store the TIP pipette in the hanging hole 18 after collecting the sample, so that the second TIP pipetting system 12 may be used to transfer the same sample.
In a specific implementation, after the sample bottle is collected by the first TIP pipetting system 11, the pipette TIP 16 can be moved to the position of the TIP pipette temporary storage rack 17, the TIP pipette is temporarily stored in the hanging hole 18, the TIP pipette can be hung in the hanging hole 18, when the same sample is filtered and needs to be pipetted, the pipette TIP 16 of the second TIP pipetting system 12 can be moved to the position corresponding to the hanging hole 18, and the same sample is adsorbed by using the temporarily stored TIP pipette for pipetting operation, so that the consumption of the TIP pipette can be reduced, and the cost is reduced.
As a further embodiment, a plurality of hanging holes 18 may be provided, so as to meet the transfer requirement of multiple samples.
As a further embodiment, the TIP pipette temporary storage rack 17 is further provided with a cleaning system, an outlet end of the cleaning system is located at an upper end of an inner wall of the hanging hole 18, and a plurality of outlets can be provided. Furthermore, the cleaning system can use medium such as gas, liquid and the like to flush the inner wall of the hanging hole, so that the problem that different samples are polluted due to the contamination of the samples in the hanging hole 18 after the TIP pipette is hung is avoided, and the sample detection is invalid. Specifically, the hanging hole 18 is a blind hole, and a return pipeline is communicated with the bottom of the blind hole for recovering samples or cleaning media.
In a specific application, a second recovery cylinder B is further disposed on the apparatus main body 1 and in the moving track of the pipette TIP 16 of the second TIP pipetting system 12, and a recovery box is disposed in the apparatus main body 1 and is communicated with the second recovery cylinder B. When the second TIP pipetting system 12 has completed pipetting, it may be moved to the second recovery cylinder B and the used TIP pipette discarded to the second recovery cylinder B for recovery.
As shown in fig. 9-11, the cell sap filtering system comprises a first bracket 19, a rotating shaft 20 is arranged on the first bracket 19, and at least one clamping jaw assembly 21 for bearing a filtering cup is fixed on the rotating shaft 20, wherein the filtering cup is used for containing samples transferred by a pipetting system; the rotating shaft 20 is also connected with a first driving mechanism 22, and the first driving mechanism 22 is used for driving the rotating shaft 20 to rotate; the rotating shaft 20 is also sleeved with a rotating frame 23, the rotating frame 23 is connected with a second driving mechanism 24, and the second driving mechanism 24 is used for driving the rotating frame 23 to rotate relative to the rotating shaft 20; still be provided with flexible subassembly on the rotating turret 23, flexible end of flexible subassembly is connected with closing plate 25, closing plate 25 is used for sealing the filter bowl, still be provided with the pressurization pipeline 26 on the closing plate 25, the pressurization pipeline is provided with the multichannel and every way pressurization pipeline 26 all runs through closing plate 25, every way pressurization pipeline 26 all corresponds alone behind the penetration of closing plate 25 one the filter bowl.
When the device is implemented, the second driving mechanism 24 is started to drive the rotating frame 23 to rotate in an initial state, so that the sealing plate 25 and the clamping jaw assembly 21 are staggered, a filter cup for cell liquid filtration can be placed on the clamping jaw assembly 21, after the completion, a pipetting system can adsorb samples into the filter cup, at the moment, the second driving mechanism 24 is started to drive the rotating frame 23 to rotate to be opposite to the clamping jaw assembly 21 again, at the moment, the telescopic assembly is started to push the sealing plate 25 to the filter cup to tightly press and seal the filter cup, the pressurized pipeline 26 can be used for pressurizing the inside of the filter cup after the sealing, the filtering effect is improved, meanwhile, the telescopic assembly is kept at the current position, the second driving mechanism 24 is closed, the first driving mechanism 22 is started to drive the rotating shaft 20 to rotate reciprocally, the clamping jaw assembly 21 can swing reciprocally along with the clamping jaw assembly, in the process, the telescopic assembly can tightly press the sealing plate 25 on the end face of the filter cup, the rotating frame 23 can follow the assembly 21 to rotate reciprocally, the whole process of the filter cup can be sealed, after the continuous design time, the first driving mechanism 22 can be stopped to return to the initial position, the telescopic assembly is retracted, the telescopic assembly can stagger the rotating frame 23, the filter cup 23 can be used for pressurizing the filter cup, the pipetting system can be moved reciprocally, and the pressurized by the pressurized pipeline can be combined, and the implementation mode can be realized, and the filtering effect can be realized.
When the rotary rack is specifically applied, the first bracket 19 and the rotary rack 23 are both U-shaped brackets, the rotary shafts 20 are arranged on two opposite side plates of the first bracket 19, two ends of each rotary rack penetrate through the first bracket 19, two side plates of the rotary rack 23 are rotationally connected with two ends of the rotary shafts 20 penetrating through the first bracket 19, the clamping jaw assemblies 21 are arranged on the inner sides of two side plates of the first bracket 19, the sealing plates 25 are also arranged on the inner sides of two side plates of the rotary rack 23, the sealing plates 25 and the clamping jaw assemblies 21 are oppositely arranged, the telescopic assemblies are arranged between the sealing plates 25 and the bottom wall of the inner side of the rotary rack 23, the sealing plates 25 can be driven to translate relative to the clamping jaw assemblies 21, and meanwhile, the pressurizing pipelines 26 can be arranged in equal quantity corresponding to the quantity of the clamping jaw assemblies 21.
As a further embodiment, the sealing plate 25 is further provided with a mounting groove corresponding to one side of the clamping jaw assembly 21, a detachable soft pad 27 is mounted in the mounting groove, each clamping jaw assembly 21 can correspond to one soft pad 27, and when the filter cup is sealed, the soft pad 27 and the cup rim of the filter cup are extruded and deformed, so that a good sealing effect can be achieved.
As a further embodiment, the first drive mechanism 22 and the second drive mechanism 24 are each reciprocating motors. Specifically, the output end of the first driving mechanism 22 is in transmission connection with the rotating shaft 20 through a belt pulley structure, the outer wall of the rotating frame 23 is located outside the rotating shaft 20 and is coaxially and fixedly provided with a fixed wheel 28, the output shaft of the second driving mechanism 24 is provided with a driving wheel 29, and transmission is realized between the fixed wheel 28 and the driving wheel 29 through a transmission belt, so that the rotating frame 23 can realize follow-up and simultaneously realize independent rotation of the rotating frame 23.
As a further embodiment, a guiding cylinder 30 is further disposed at the bottom of the rotating frame 23, a guiding rod 31 is further disposed on the side wall of the closing plate 25, the guiding rod 31 penetrates the guiding cylinder 30 and can slide along the guiding cylinder 30, and the axes of the guiding rod 31 and the guiding cylinder 30 are the same as the telescopic direction of the telescopic assembly. Further, the guide bar 31 may assist in the movement of the closing plate 25 under the restriction of the guide cylinder 30. Specifically, the both sides of closure plate 25 all are provided with guide bar 31, flexible subassembly then sets up in the middle part position of both sides guide bar 31, and then, guide bar 31 can also further guarantee the balanced extrusion of closure plate 25 to the filter bowl, guarantees that the filter bowl is all pressed evenly and can seal completely.
In a specific application, two clamping jaw assemblies 21 are arranged at intervals along the length direction of the rotating shaft 20.
As a further embodiment, the clamping jaw assembly 21 comprises a filter cup positioning seat, a driving end connected to the filter cup positioning seat, and an actuating end connected to the driving end, wherein the driving end is used for driving the actuating end to move relatively so as to clamp or unclamp the filter cup; the filter cup positioning seat is provided with a filter cup positioning hole, the execution end is provided with two clamping jaws which are arranged oppositely, and the two clamping jaws are positioned outside the filter cup positioning hole and are all connected with arc clamping plates. When implementing, the filter bowl passes through filter bowl locating hole location on the filter bowl locating seat, and the clamping jaw of drive end drive both sides is close to relatively, and then the usable both sides arc splint clamp tightly at the filter bowl outer wall, realizes the firm centre gripping of filter bowl, and overall structure is simple, can realize the quick clamp of filter bowl. Specifically, the driving end is a bidirectional driving cylinder. Specifically, the radian of the arc clamping plate is the same as the radian of the outer wall of the cup body of the filter cup, so that the arc clamping plate and the outer wall of the cup body are more attached during clamping, and the clamping effect is further improved.
It should be noted that, the filter bowl is a bowl body with an inner double-layer structure, the filter bowl is divided into an upper layer and a lower layer by the filter membrane, effective cell tissues after filtration are left on the upper layer so as to facilitate subsequent pipetting, a specific filter bowl structure is common in the prior art, the application of the specific filter bowl structure is wide in the field of tabletting, the specific structure and the filtration principle of the specific filter bowl structure are not particularly accumulated any more, and the specific implementation of the specific filter bowl structure can be implemented by a person skilled in the art based on the prior art literature and common knowledge without affecting the specific implementation of the specific filter bowl structure.
As shown in fig. 12, the clamping jaw assemblies 21 and the telescopic assemblies are all driven by adopting a pneumatic structure; the cell sap filtering system also comprises a pneumatic control system; the pneumatic control system comprises a gas source 32, a filter valve 33 and a first three-way valve 34 which are communicated, wherein two outlets of the first three-way valve 34 are respectively communicated with a control loop 35 and a pressurizing loop 36; the control circuit 35 comprises a plurality of middle sealing electromagnetic valves 37 which are communicated with the outlet of the first three-way valve 34, and the outlet of each middle sealing electromagnetic valve 37 is independently communicated with the clamping jaw assembly 21 or the telescopic assembly; the pressurizing circuit 36 includes a first two-way valve 38, a precise pressure reducing valve 39 and a plurality of second two-way valves 40, wherein the first two-way valve 38 is communicated with the outlet of the first three-way valve 34, the plurality of second two-way valves 40 are communicated with the outlet of the precise pressure reducing valve 39, and the outlet end of each second two-way valve 40 is independently communicated with one pressurizing pipeline 26.
Based on this, the present embodiment can simultaneously realize and control the functions of the clamping jaw assembly 21, the telescopic assembly and the pressurizing pipeline 26 by using the pneumatic control system, thereby simplifying the control system difficulty and the control flow of the overall filtering system, and the control is more conveniently realized by adopting the same medium-gas, and simplifying the control loop.
It should be noted that, in the control circuit 35, in order to ensure that each middle sealing electromagnetic valve 37 can be independently communicated with the clamping jaw assembly 21 or the telescopic assembly, when the number of the clamping jaw assemblies 21 or the telescopic assemblies is large, the outlet end of the first three-way valve 34 may be provided with a corresponding three-way valve, a multi-way valve, etc. for communicating with each middle sealing electromagnetic valve 37, so that the flow regulation of each shunt or the shunt requirement of the air path can be realized through the corresponding three-way valve, multi-way valve, etc.; similarly, in the pressurizing circuit 36, a corresponding three-way valve, a multi-way valve, etc. can be added to the outlet end of the precise pressure reducing valve 39 for communicating with each second two-way valve 40, so as to realize flow regulation or realize the branching requirement of the gas path. For example, when two clamping jaw assemblies 21 are provided, three paths of air paths are required by adding the telescopic assembly, a four-way valve can be added at the moment, three outlets of the four-way valve are respectively communicated with the middle sealing electromagnetic valve 37, the air path quantity requirement can be achieved, and similarly, two clamping jaw assemblies 21 are provided, two corresponding pressurizing pipelines 26 are provided, a second three-way valve can be added at the moment, and two outlets of the second three-way valve are respectively communicated with the second two-way valve 40, so that the air path quantity requirement and flow control of the pressurizing pipelines 26 can be achieved.
With continued reference to fig. 6, the cell sap filtration system further includes a filter cup transport system; the filter cup transferring system comprises a filter cup positioning and conveying device and a filter cup transferring device; the filter cup positioning and conveying device comprises a second conveying belt 41 and a second positioning plate 42 fixed above the second conveying belt 41, wherein a plurality of second through grooves 43 for positioning filter cups are formed in the second positioning plate 42, and the second through grooves 43 are formed in the conveying direction of the second conveying belt 41; the filter cup transfer device comprises a second two-axis moving mechanism 44 and a filter cup clamping jaw 45 connected with the second two-axis moving mechanism 44, wherein the filter cup clamping jaw 45 is used for grabbing a filter cup and transferring a sample filter cup onto the clamping jaw assembly 21 based on the second two-axis moving mechanism 44.
In practice, the filter cup is placed on the second through slot 43, and is transported by the second conveyor belt 41 to move along the second through slot 43 to the grabbing position, and the second two-axis moving mechanism 44 can drive the filter cup clamping jaw 45 to the grabbing position and clamp the filter cup onto the clamping jaw assembly 21, so as to complete placement of the filter cup.
When the device is specifically applied, the third recovery cylinder C is further arranged in the moving track of the filter cup clamping jaw 45 on the device main body 1, the recovery box communicated with the third recovery cylinder C is further arranged inside the device main body 1, after the sample transfer of the filter cup on the clamping jaw assembly 21 is completed, the filter cup clamping jaw 45 can clamp the filter cup and discard the filter cup to the third recovery cylinder C, so that the filter cup is intensively recovered, and the placement of the next filter cup is not affected.
13-18, the slide feeding and coding system comprises a slide mounting system, a multi-station turntable system and a laser coding machine (not shown in the figures); the multi-station turntable system comprises a rotation driving mechanism 46 and a turntable 47 connected with the rotation driving mechanism 46, wherein a plurality of slide glass placing grooves 48 are circumferentially distributed on the turntable 47 at intervals, each slide glass placing groove 48 is provided with a sedimentation cup clamping plate 49, and the sedimentation cup clamping plates 49 are opposite to the slide glass placing grooves 48 up and down and form a gap therebetween; a sedimentation cup mounting hole 50 vertically penetrates through the middle of the sedimentation cup clamping plate 49, and two first through-hole grooves 51 are oppositely formed in the side wall of the sedimentation cup mounting hole 50; the slide mounting system comprises a second bracket 52, a slide feeding box 53 is mounted on the second bracket 52, the slide feeding box 53 is provided with a feeding cavity 54 for stacking slides, the lower end of the slide feeding box 53 is horizontally provided with a discharging channel, and the discharging channel is communicated with the feeding cavity 54; the second bracket 52 is also provided with a linear driving mechanism 55 and a push plate 56 connected with the moving end of the linear driving mechanism 55, and the push plate 56 penetrates through the slide feeding box 53 and can extend out through the outlet end of the discharging channel so as to push out the slide in the slide feeding box 53 into the slide placing groove 48; the turnplate 47 is positioned below each slide glass placing groove 48 and is also provided with a notch 57 in a penetrating way, the laser coding machine is arranged below the turnplate 47, and the laser emitting end of the laser coding machine is opposite to the slide glass through the notch 57.
In practice, the turntable 47 can rotate circumferentially under the drive of the rotation driving mechanism 46, when the turntable rotates to the slide mounting system and stops, at least one slide placing groove 48 is opposite to the discharge channel, at this time, the linear driving mechanism 55 is started, the slide pre-stored in the feed cavity 54 is pushed out to the discharge channel through the push plate 56 until the slide completely enters the slide placing groove 48, the slide mounting can be completed, at this time, the turntable 47 rotates again and stops mounting the sedimentation cup in the sedimentation cup mounting hole 50 and fixing, at this time, the turntable 47 continues to rotate to the slide in the sedimentation cup in the moving track of the pipette gun head 16 of the second TIP pipetting system 12, the turntable continues to rotate to the laser coding machine, the laser coding machine carries out laser coding on the slide added with the sample, the corresponding information code is ensured on the slide of each sample, and the slide feeding coding can be continuously completed by repeating the steps.
It should be noted that, the sedimentation cup is the cup structure with both ends open, and its one end opening outside symmetry is provided with two ear plates, and when the installation, two ear plates get into sedimentation cup mounting hole 50 through two first through hole grooves 51 earlier, after getting into completely, rotate again and subside the cup and make two ear plates rotate to in the clearance and stagger two first through hole grooves 51, can with sedimentation cup chucking.
In practice, the feeding chamber 54 in the slide feeding box 53 is vertically disposed and has an upper end and a lower end open, the lower end open is communicated with the discharge channel, slides can be stacked in the feeding chamber 54, and the pushing plate 56 can horizontally enter the discharge channel to push out the slide at the lowest end onto the turntable 47.
As a further embodiment, the pusher plate 56 is a flat plate, the discharge channel is a square channel extending through the slide feed cassette 53, and the flat plate and square channel are the same height and width as the slides. Based on this, push plate 56 is in the propelling movement in-process, and its single only can push a slide glass, and every slide glass all can be according to the ejection of compact passageway direction ejection of compact, has not only ensured uniqueness and the validity of slide glass installation, also can avoid slide glass and its mutual screens when push plate 56 makes a round trip to push away the material and influence its work, has improved overall structure's running stability.
In specific implementation, the outlet end of the discharging channel is spaced from the edge of the turntable 47 by a distance less than the length of the slide, and the outlet end of the discharging channel is flush with the slide placement groove 48. Through the design, the glass slide can keep the installation state with the same height and angle as the glass slide placing groove 48 in the process of entering the glass slide placing groove 48, so that the glass slide can smoothly enter the glass slide placing groove 48, and the installation precision and effect of the glass slide can be further ensured.
With continued reference to fig. 15, the slide supply coding system further includes a sedimentation cup transfer system; the sedimentation cup transfer system comprises a chain plate conveying device and a sedimentation cup transfer device; the chain plate conveying device comprises a chain plate driving mechanism 58 and a conveying chain plate assembly 59 in transmission connection with the chain plate driving mechanism 58, wherein the chain plates of the conveying chain plate assembly 59 are respectively provided with a sedimentation cup positioning groove 60, a plurality of sedimentation cup positioning grooves 60 are arranged at intervals along the length direction of the chain plate, and two second vent grooves 61 are oppositely arranged on the side wall of each sedimentation cup positioning groove 60; the sedimentation cup transferring device comprises a third two-axis moving mechanism 62 and a sedimentation cup rotating clamping jaw 63 connected with the third two-axis moving mechanism 62, wherein the sedimentation cup rotating clamping jaw 63 is used for grabbing a sedimentation cup, and based on the third two-axis moving mechanism 62, a sample sedimentation cup is transported into the sedimentation cup mounting hole 50, and the sedimentation cup is fixed in the sedimentation cup mounting hole 50 through rotation.
In the implementation, the sedimentation cup is pre-installed in the sedimentation cup positioning groove 60, and alignment clamping is carried out through the two second through-hole grooves 61 and the two lug plates, not only can the sedimentation cup be fixed, and the position of the sedimentation cup can be limited in advance, the control of the rotation angle of the rotation clamping jaw 63 of the subsequent sedimentation cup is facilitated, the installation of the sedimentation cup in the sedimentation cup installation hole 50 is facilitated, when the sedimentation cup is used, the chain plate driving mechanism 58 drives the conveying chain plate assembly 59 to rotate, the sedimentation cup fixed on the chain plate driving mechanism can move to the grabbing position of the rotation clamping jaw 63 of the sedimentation cup, the sedimentation cup is grabbed and installed in the sedimentation cup installation hole 50 by the rotation clamping jaw 63 of the sedimentation cup, and the initial position of the sedimentation cup, namely the positions of the two lug plates are determined, so that the two lug plates can rotate in advance by a certain angle when installed, the two lug plates are right opposite to the first through-hole grooves 51, and then rotate again after entering a gap, the two lug plates are staggered, the first through-hole grooves 51 can be used for fixedly installing the sedimentation cup, and automatic installation of the sedimentation cup is realized.
As a further embodiment, the height of the commercial gap between the sedimentation cup clamping plate 49 and the slide placing groove 48 is the same as the thickness of the clamping plate, so that the sedimentation cup clamping plate can be completely clamped on the slide glass after the sedimentation cup is installed, leakage of a sample or a medium used for subsequent sedimentation dyeing is avoided, and a good isolation effect is achieved.
With continued reference to fig. 14, the multi-station sedimentation dyeing system includes a plurality of dyeing apparatuses sequentially arranged along the circumferential direction of the turntable 47; the dyeing equipment comprises a lifting rotating assembly 64 and a pump head assembly 65 connected with the lifting rotating assembly 64, wherein the pump head assembly 65 is used for conveying corresponding liquor into a sedimentation cup to dye and clean a sample and sucking the liquor.
In practice, the sample needs to undergo multiple steps of sedimentation, fixing, hematoxylin dyeing, buffering, EA dyeing, cleaning, wetting and the like, so that in this embodiment, by combining with the turntable 47, multiple dyeing devices for implementing different dyeing steps are sequentially arranged in the circumferential direction of the turntable 47, and then rapid sedimentation dyeing operation can be sequentially performed on the sample, so that not only can the device structure and the procedure be simplified, but also the uniqueness of the procedure sequence can be ensured, and the method has high stability.
It should be noted that, the specific lifting and rotating assembly 64 and the pump head assembly 65 may be implemented by using a dyeing apparatus structure in the prior art, and the present embodiment will not be described herein in detail, but it should be understood that the above structure can be implemented by those skilled in the art based on the prior art documents and common knowledge, and does not affect the specific implementation of the present embodiment.
In the concrete implementation, a fourth recovery cylinder D is further disposed on the apparatus main body 1, the fourth recovery cylinder D is located on the moving track of the sedimentation cup rotating clamping jaw 63, and a recovery box communicated with the fourth recovery cylinder D is further disposed in the apparatus main body 1. When the sedimentation cup is dyed, the turntable 47 can rotate the position where the sedimentation cup needs to be removed to the moving track of the sedimentation cup rotating clamping jaw 63, and the sedimentation cup can be clamped by the sedimentation cup rotating clamping jaw 63 and then discarded to the fourth recovery cylinder D, so that the automatic removal of the sedimentation cup is realized.
With continued reference to fig. 15, 17 and 18, the slide uv sealing system includes a base 66, a slide chute 67 is provided on the base 66, and two ends of the slide chute 67 are respectively spaced from the turntable 47 and the slide box storage assembly system; the base 66 is provided with a curing agent adding system 68 and an ultraviolet lamp irradiating system 69 in sequence along the upper part of the slide chute 67; the bottom of the slide chute 67 is hollowed out to form a communication port 70, the base 66 is further provided with a lifting module 71, a linear module 72 connected to the lifting module 71, and a slide push-pull plate 73 connected to the linear module 72, and the slide push-pull plate 73 can push a slide from the turntable 47 to the slide chute 67 and push the slide to the slide box storage assembly system along the slide chute 67 under the action of the lifting module 71 and the linear module 72.
In implementation, the slide push-pull plate 73 stretches into the notch 57 under the action of the lifting module 71 and the linear module 72, then the slide is pushed out of the slide placing groove 48 from the turntable 47 and enters the slide chute 67, and is continuously pushed to sequentially pass through the curing agent adding system 68 and the ultraviolet lamp irradiation system 69, the curing agent is added to a sample on the slide through the curing agent adding system 68, then the ultraviolet lamp irradiation system 69 is used for carrying out ultraviolet sealing on the curing agent, the sample is completely cured on the slide, finally, the slide after curing is pushed to the slide box storage assembly system for storage through the slide push-pull plate 73, the slide displacement operation can be realized only by operating the slide push-pull plate 73 in the whole process, the slide can be kept in a horizontal state in the whole displacement process, the position and state of the sample on the slide can be protected, meanwhile, the slide can be well positioned under the limitation of the slide chute 67 in the displacement process, the curing agent adding and the ultraviolet lamp irradiation can be corresponding to the sample area, the effect of the ultraviolet sealing can be improved, the quick transfer and the whole slide can be realized, and the whole slide production efficiency can be improved, and the slide production equipment can be used.
It should be noted that, the curing agent adding system 68 and the ultraviolet lamp irradiating system 69 may be implemented by corresponding structures in the prior art, and the ultraviolet sealing sheet structure in the prior art is also fully applicable to the present embodiment, so that the specific curing agent adding system 68 and the ultraviolet lamp irradiating system 69 are not described in detail in this embodiment, that is, the present embodiment is not described in detail here, and it should be understood that the above structures can be implemented by those skilled in the art based on the prior art documents and common knowledge, and do not affect the specific implementation of the present embodiment.
As a specific embodiment, the width and height of the slide chute 67 are the same as the width and thickness of the slide. Further, the slide is positioned in the slide chute 67 with a better positioning effect, and can be ensured to be displaced accurately along the slide chute 67 in the setting direction.
In a specific application, the slide push-pull plate 73 is formed with a baffle 74 protruding upwards along both ends and the middle part of the slide push-pull plate in the length direction, and a first limit groove 75 and a second limit groove 76 are formed between the three baffles 74.
In practice, before the slide glass push-pull plate 73 enters the notch, the slide glass push-pull plate 73 can be adjusted to a low position through the lifting module 71, namely at least the baffle plate 74 is lower than the bottom of the slide glass, after the slide glass push-pull plate 73 enters the notch and is in place, the lifting module 71 controls the slide glass push-pull plate 73 to ascend, at the moment, the slide glass is positioned in the first limit groove 75 and limited by the baffle plate 74 at the outer side end part of the first limit groove 75, the slide glass can translate along with the slide glass push-pull plate 73 and can well enter the slide glass chute 67, after the slide glass stops corresponding operation when the slide glass is positioned at the position of the curing agent adding system 68, the slide glass push-pull plate 73 can descend again to enable the second limit groove 76 to be positioned below the corresponding slide glass, the slide glass is pushed to the ultraviolet lamp irradiation system 69 by the second limit groove 76 after the slide glass push-pull plate 73 is ascended, and the slide glass push-pull plate 73 can be prevented from entering the notch again due to the length problem in the pushing process, affecting the normal operation of the turntable 47, when the ultraviolet lamp irradiation system 69 is completed, the slide push-pull plate 73 descends again and makes the baffle plate 74 at the outer side end part of the second limit groove 76 block at one side of the slide, the slide is pushed by the baffle plate 74 to slide along the slide chute 67 until entering the slide box storage assembly system in ascending, the whole-course automatic conveying of the slide can be realized, in the conveying process, the slide push-pull plate 73 realizes the conveying of different slides at different stages by respectively utilizing different structures and positions on the slide push-pull plate 73 based on the actions of the lifting module 71 and the linear module 72, not only the smooth conveying of the slide can be realized, but also the two ends of the slide push-pull plate 73 are prevented from extending into the turntable 47 or the slide ultraviolet sealing system in the conveying process to influence the two operations, the design length of the slide push-pull plate 73 can be reduced so as to reduce the volume or the occupied space requirement of the whole structure of the slide ultraviolet sealing system, and the slide ultraviolet sealing system is miniaturized.
As a further embodiment, the slide push-pull plate 73 is disposed below the slide chute 67 and along the opening direction of the slide chute 67, and the width of the slide push-pull plate 73 is smaller than or equal to the width of the communication port 70. In application, the slide push-pull plate 73 is arranged below the slide chute 67 and along the direction of the slide chute 67 in order to avoid affecting the lifting operation, and the width of the slide push-pull plate 73 is smaller than or equal to the width of the communication port 70, so that the slide push-pull plate 73 can translate and lift along the communication port 70 below the slide chute 67, the space of the slide chute 67 and the rest upper space are not occupied, and the slide can be supported in the pushing process, so that the pushing and the horizontal state maintaining of the slide are further ensured.
As a further embodiment, the length of each of the first and second limiting grooves 75 and 76 is equal to or greater than the length of the slide; the widths of the slide push-pull plates 73 in the areas where the first limiting groove 75 and the second limiting groove 76 are located are smaller than the width of the communication port 70, and the width of the slide push-pull plates 73 in the area where the first limiting groove 75 is located is smaller than or equal to the width of the notch 57 below the slide placing groove 48. Based on this, the lengths of the first limiting groove 75 and the second limiting groove 76 are equal to or greater than the length of the slide, so that the slide can be stably limited between the two side baffles 74 corresponding to the limiting grooves, a good positioning effect can be achieved on the slide, similarly, the width of the slide push-pull plate 73 is smaller than the width of the communication port 70, the corresponding lifting and shifting operation of the slide push-pull plate 73 can be facilitated, the width of the slide push-pull plate 73 in the area where the first limiting groove 75 is located is equal to or less than the width of the notch 57 below the slide placing groove 48, so that the slide push-pull plate 73 can well enter the lower side of the slide placing groove 48 through the notch 57 and lift, the slide can be withdrawn more safely, and the fault tolerance is higher.
With continued reference to fig. 15, the slide box storage assembly system includes a lifting base 77 and a slide box 78 movably connected to the lifting base 77, the slide box 78 is provided with at least one opening 79 for the slide to enter, and a plurality of rows of clamping grooves 80 are symmetrically arranged in the slide box 78 along the height direction thereof.
In practice, before the slide glass enters the slide box storage assembly system, the lifting base 77 can drive the slide box 78 to lift, so that any row of clamping grooves 80 symmetrically arranged in the slide glass can be flush with the slide glass entering position such as the slide glass sliding groove 67, and after the slide glass enters the slide box 78 from the opening 79, the slide glass can be clamped in the corresponding row of clamping grooves 80, and in this way, after all the clamping grooves 80 store the slide glass, the slide box 78 can be taken down for replacing the slide box 78.
Specifically, the lifting end of the lifting base 77 is provided with a positioning seat, the slide box 78 is positioned on the positioning seat, and the lifting end of the lifting base 77 is also provided with a clamping mechanism for clamping the slide box 78. The clamping mechanism can position and clamp the glass sheet box 78 on the positioning seat and can realize rapid assembly and disassembly of the glass sheet box 78. Further, the clamping mechanism may be a threaded post screwed through a support plate, where the support plate is connected to the lifting end, and the threaded post may be pushed against the upper end of the cassette 78 to fix the whole onto the positioning seat.
The above is the whole content of the intelligent pathology sheeting system, and based on the intelligent pathology sheeting system, the second embodiment of the invention also provides an intelligent pathology sheeting method, which comprises the following steps:
S1
the sample bottle code scanning and cover opening system transfers the sample bottles to a sample bottle bearing seat 9 of the pipetting system, performs code scanning and cover opening operation on the sample bottles, and synchronously transfers the filter cup to a clamping jaw assembly 21 for clamping by a filter cup transferring system;
S2
the first TIP pipetting system 11 adsorbs the sample in the sample bottle on the sample bottle bearing seat 9 to the filter cup;
S3
the second driving mechanism 24 drives the rotating frame 23 to rotate relative to the rotating shaft 20, the sealing plate 25 is rotated to a position opposite to the cup opening of the filter cup, the telescopic assembly starts to drive the sealing plate 25 to move until the sealing cup opening is pressed, the pressurizing pipeline 26 blows air into the filter cup to pressurize and keep a pressurized state, the first driving mechanism 22 is started to drive the rotating shaft 20 to rotate after the completion, the clamping jaw assembly 21 and the rotating frame 23 are driven to synchronously rotate in a circumferential reciprocating manner, the first driving mechanism 22 stops after the rotation design time, and the second driving mechanism 24 is started to drive the rotating frame 23 to rotate relative to the rotating shaft 20 and stagger the filter cup;
S4
the slide mounting system conveys the slide to the slide placing groove 48, the rotary disc 47 rotates to the position of the sedimentation cup transfer system, the sedimentation cup transfer system conveys the sedimentation cup to the sedimentation cup mounting hole 50 and clamps the sedimentation cup through the first through hole groove 51, the rotary disc 47 rotates again to rotate the slide to the position of the second TIP pipetting system 12, the second TIP pipetting system 12 adsorbs samples in the filter cup on the clamping jaw assembly 21 to the slide on the rotary disc 47, the rotary disc continuously rotates and sequentially performs coding through the position of the laser coding machine, the multi-station sedimentation dyeing system performs sedimentation dyeing and then conveys the sedimentation cup to the slide ultraviolet sealing system for sedimentation dyeing, and the rotary disc 47 continuously rotates to the slide ultraviolet sealing system after completion;
S5
The slide push-pull plate 73 extends to the slide chute 67 and extends below the slide placing groove 48, and rises synchronously, so that the slide is positioned in a first limit groove 75 of the slide push-pull plate 73, the slide push-pull plate 73 moves back to hook the slide on the slide chute 67 by using a baffle 74 at the outer end part and is positioned below the curing agent adding system 68, after the curing agent is added to a sample area on the slide by the curing agent adding system 68, the slide push-pull plate 73 moves downwards and synchronously translates a second limit groove 76 below the slide, then moves upwards to limit the slide in the second limit groove 76, after the slide push-pull plate 73 moves horizontally to transfer the slide below the ultraviolet lamp irradiating system 69 for ultraviolet sealing, after the slide push-pull plate 73 moves downwards and synchronously translates a baffle 74 at the outer end part of the second limit groove 76 to one side of the slide, and then moves upwards and horizontally, and pushes the slide to the slide box storage assembly system by using the baffle 74 at the outer end part of the second limit groove 76;
S6
the slide cassette storage assembly system adjusts the height of the slide cassette 78 so that any row of clamping grooves 80 in the slide cassette 78 are aligned with slides, and the slide push-pull plate 73 pushes the slides from the openings 79 of the slide cassette 78 into the slide cassette 78 and are clamped in the corresponding clamping grooves 80 for storage, thus finishing slide preparation.
The foregoing description of the embodiments has been provided for the purpose of illustrating the general principles of the invention, and is not meant to limit the scope of the invention, but to limit the invention to the particular embodiments, and any modifications, equivalents, improvements, etc. that fall within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (8)
1. An intelligent pathological imaging system comprises a device main body, and is characterized in that a sample bottle scanning and uncovering system, a liquid transferring system, a cell liquid filtering system, a glass slide feeding and coding system, a multi-station sedimentation dyeing system, a glass slide ultraviolet sealing system and a glass slide box storage assembly system are arranged on the device main body;
the sample bottle code scanning and cover opening system is used for transferring the sample bottles to the liquid transferring system and executing code scanning and cover opening operation on the sample bottles;
the pipetting system is used for transferring samples in the sample bottles to the cell liquid filtering system for filtering, and transferring the filtered samples to the glass slide feeding and coding system;
the cell fluid filtering system is used for filtering a sample to extract effective cell fluid;
the slide glass feeding and coding system is used for providing slide glass to bear samples, coding the slide glass and sequentially conveying the slide glass to the multi-station sedimentation and dyeing system and the slide glass ultraviolet sealing system;
The multi-station sedimentation dyeing system is used for carrying out sedimentation dyeing on a sample on a glass slide;
the slide ultraviolet sealing system is used for performing ultraviolet sealing on the sample on the sedimentation-dyed slide;
the slide box storage assembly system is used for collecting and storing the slide glass subjected to ultraviolet sealing;
wherein,
the cell liquid filtering system comprises a first bracket, a rotating shaft is arranged on the first bracket, at least one clamping jaw assembly used for bearing a filtering cup is fixed on the rotating shaft, and the filtering cup is used for containing samples transferred by the pipetting system; the rotating shaft is also connected with a first driving mechanism, and the first driving mechanism is used for driving the rotating shaft to rotate; the rotating shaft is also sleeved with a rotating frame, the rotating frame is connected with a second driving mechanism, and the second driving mechanism is used for driving the rotating frame to rotate relative to the rotating shaft; the rotary frame is also provided with a telescopic component, the telescopic end of the telescopic component is connected with a sealing plate, the sealing plate is used for sealing the filter cups, the sealing plate is also provided with a pressurizing pipeline, the pressurizing pipeline is provided with a plurality of paths, each path of pressurizing pipeline penetrates through the sealing plate, and each path of pressurizing pipeline independently corresponds to one filter cup after penetrating through the sealing plate;
The slide glass supplying and coding system comprises a slide glass mounting system, a multi-station turntable system and a laser coding machine; the multi-station turntable system comprises a rotation driving mechanism and a turntable connected with the rotation driving mechanism, wherein a plurality of glass slide placing grooves are circumferentially distributed on the turntable at intervals, each glass slide placing groove is provided with a sedimentation cup clamping plate, and the sedimentation cup clamping plates are opposite to the glass slide placing grooves up and down and form a gap therebetween; the middle part of the sedimentation cup clamping plate vertically penetrates through a sedimentation cup mounting hole, and two first through-hole grooves are oppositely formed in the side wall of the sedimentation cup mounting hole; the slide glass mounting system comprises a second bracket, a slide glass feed box is mounted on the second bracket, a feed cavity for stacking slide glass is formed in the slide glass feed box, a discharge channel is horizontally formed in the lower end of the slide glass feed box, and the discharge channel is communicated with the feed cavity; the second bracket is also provided with a linear driving mechanism and a push plate connected with the moving end of the linear driving mechanism, and the push plate penetrates through the slide feeding box and can extend out through the outlet end of the discharging channel so as to push out the slide in the slide feeding box into the slide placing groove; the carousel is located every slide glass standing groove's below still runs through and is provided with the breach, the laser coding machine sets up in the carousel below, and its laser emission end pass through the breach with the slide glass is relative.
2. The intelligent pathology sheeting system according to claim 1, wherein the sample vial code scanning and uncapping system comprises a sample vial positioning and conveying device, a sample vial transferring device and a scanner;
the sample bottle positioning and conveying device comprises a first conveying belt and a first positioning plate fixed above the first conveying belt, wherein a plurality of first through grooves for positioning sample bottles are formed in the first positioning plate, and the first through grooves are formed in the conveying direction of the first conveying belt;
the sample bottle transferring device comprises a first two-axis moving mechanism and a sample bottle clamping jaw connected with the first two-axis moving mechanism, wherein the sample bottle clamping jaw is used for grabbing a sample bottle and transferring the sample bottle to a pipetting system based on the first two-axis moving mechanism;
the scanner is arranged in the moving track of the first two-axis moving mechanism, and is used for scanning the information code outside the sample bottle after the sample bottle is transported to the pipetting system.
3. The intelligent pathology sheeting system of claim 1, wherein the pipetting system comprises a vial carrier, a TIP pipette carrier, a first TIP pipetting system, and a second TIP pipetting system;
the sample bottle bearing seat comprises a pneumatic rotary clamping jaw arranged on one side of the TIP pipette bearing seat, and the clamping jaw end of the pneumatic rotary clamping jaw is used for grasping a sample bottle and driving the sample bottle to rotate under the action of the rotating end of the pneumatic rotary clamping jaw;
The TIP pipette bearing seat comprises a supporting frame, wherein a plurality of mounting grooves are formed in the supporting frame and are used for mounting a TIP pipette tray, and a plurality of TIP pipette placing holes are formed in the TIP pipette tray;
the first TIP pipetting system is used for adsorbing the sample in the sample bottle to the cell sap filtering system;
the second TIP pipetting system is used for transferring the sample filtered by the cell liquid filtering system to a glass slide supply coding system;
the TIP pipette tray is disposed within a track range of pipette TIPs of the first TIP pipetting system and the second TIP pipetting system.
4. The intelligent pathology sheeting system of claim 3, wherein the TIP pipetting system further comprises a TIP pipette staging rack having a plurality of hanging holes for hanging TIP pipettes disposed thereon; after the first TIP pipetting system collects the sample, the TIP pipette can be temporarily stored on the hanging hole for the second TIP pipetting system to use when the same sample is transferred.
5. The intelligent pathology sheeting system of claim 1, wherein the cytosolic filtration system further comprises a filter cup transfer system;
the filter cup transferring system comprises a filter cup positioning and conveying device and a filter cup transferring device;
The filter cup positioning and conveying device comprises a second conveying belt and a second positioning plate fixed above the second conveying belt, wherein a plurality of second through grooves for positioning filter cups are formed in the second positioning plate, and the second through grooves are formed in the conveying direction of the second conveying belt;
the filter cup transferring device comprises a second two-axis moving mechanism and a filter cup clamping jaw connected with the second two-axis moving mechanism, wherein the filter cup clamping jaw is used for grabbing a filter cup and transferring a sample filter cup to a clamping jaw assembly based on the second two-axis moving mechanism.
6. The intelligent pathology sheeting system of claim 1, wherein the slide feeding and coding system further comprises a sedimentation cup transfer system;
the sedimentation cup transfer system comprises a chain plate conveying device and a sedimentation cup transfer device;
the chain plate conveying device comprises a chain plate driving mechanism and a conveying chain plate assembly in transmission connection with the chain plate driving mechanism, wherein the chain plates of the conveying chain plate assembly are provided with sedimentation cup positioning grooves, a plurality of sedimentation cup positioning grooves are arranged at intervals along the length direction of the chain plate, and two second through-hole grooves are formed in the side wall of each sedimentation cup positioning groove relatively;
the sedimentation cup transfer device comprises a third two-axis moving mechanism and a sedimentation cup rotating clamping jaw connected with the third two-axis moving mechanism, wherein the sedimentation cup rotating clamping jaw is used for grabbing a sedimentation cup, and the sample sedimentation cup is transported into a sedimentation cup mounting hole based on the third two-axis moving mechanism and is fixed in the sedimentation cup mounting hole through rotation.
7. The intelligent pathology sheeting system according to claim 1, wherein the slide ultraviolet sealing system comprises a base, wherein slide grooves are arranged on the base, and two ends of the slide grooves are respectively arranged at intervals with the turntable and the slide box storage assembly system;
the base is sequentially provided with a curing agent adding system and an ultraviolet lamp irradiation system along the upper part of the slide chute;
the bottom of the slide chute is hollowed out to form a communication port, the base is also provided with a lifting module, a linear module connected to the lifting module and a slide push-pull plate connected to the linear module, and the slide push-pull plate can push a slide from the turntable to the slide chute and push the slide to the slide box storage assembly system along the slide chute under the action of the lifting module and the linear module.
8. An intelligent pathology sheeting method, based on the intelligent pathology sheeting system implementation of any one of claims 1-7, characterized by comprising the following steps:
S1
the sample bottle code scanning and cover opening system transfers the sample bottle to a sample bottle bearing seat of the pipetting system, and performs code scanning and cover opening operation on the sample bottle, and the filter cup transferring system synchronously transfers the filter cup to the clamping jaw assembly for clamping;
S2
The first TIP pipetting system adsorbs samples in the sample bottles on the sample bottle bearing seat to the filter cup;
S3
the second driving mechanism drives the rotating frame to rotate relative to the rotating shaft, the sealing plate is rotated to a position opposite to the cup opening of the filter cup, the telescopic assembly starts to drive the sealing plate to move until the sealing cup opening is pressed, the pressurizing pipeline blows air into the filter cup to pressurize and keep a pressurizing state, the first driving mechanism is started to drive the rotating shaft to rotate after the pressurization pipeline blows air into the filter cup, the clamping jaw assembly and the rotating frame are driven to synchronously and circularly reciprocate, the first driving mechanism stops after the rotation design time, and the second driving mechanism is started to drive the rotating frame to rotate relative to the rotating shaft and stagger the filter cup;
S4
the glass slide mounting system conveys glass slides to a glass slide placing groove, the rotary table rotates to the position of the sedimentation cup transfer system, the sedimentation cup transfer system conveys sedimentation cups to the sedimentation cup mounting holes and clamps the sedimentation cups through the first through-hole groove, the rotary table rotates again to rotate the glass slides to the position of the second TIP liquid transfer system, the second TIP liquid transfer system adsorbs samples in the filter cups on the clamping jaw assemblies onto the glass slides on the rotary table, the rotary table continuously rotates and sequentially carries out coding at the position of the laser coding machine, the multi-station sedimentation dyeing system carries out sedimentation dyeing and then conveys the glass slides to the glass slide ultraviolet sealing system for sedimentation dyeing, and the rotary table continuously rotates to the glass slide ultraviolet sealing system after the sedimentation dyeing is completed;
S5
The slide glass push-pull plate extends to the slide glass sliding groove and extends below the slide glass placing groove, and synchronously rises to enable the slide glass to be located in a first limit groove of the slide glass push-pull plate, the slide glass push-pull plate moves back to hook the slide glass on the slide glass sliding groove by using a baffle plate at the outer end part and is located below a curing agent adding system, after the curing agent adding system adds the curing agent to a sample area on the slide glass, the slide glass push-pull plate moves downwards and synchronously translates a second limit groove to the lower part of the slide glass, then moves upwards to limit the slide glass to the second limit groove, after the slide glass push-pull plate translates to the lower part of an ultraviolet lamp irradiation system to carry out ultraviolet sealing piece, after the slide glass push-pull plate moves downwards and synchronously translates a baffle plate at the outer end part of the second limit groove to one side of the slide glass, and then moves upwards and translates, and pushes the slide glass to a slide box storage assembly system by using the baffle plate at the outer end part of the second limit groove;
S6
the slide box storage assembly system adjusts the height of the slide box so that any row of clamping grooves in the slide box are aligned with the slides, and the slide push-pull plate pushes the slides to enter the slide box from the opening of the slide box and to be clamped in the corresponding clamping grooves for storage, so that the slide preparation is completed.
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Citations (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2301551A1 (en) * | 1997-08-25 | 1999-03-04 | Monogen, Inc. | Method and apparatus for automatically forming monolayers from particulate matter separated from fluid samples |
| WO2003045560A2 (en) * | 2001-11-26 | 2003-06-05 | Lab Vision Corporation | Automated tissue staining system and reagent container |
| AU2006201456A1 (en) * | 2000-03-08 | 2006-04-27 | Cytyc Corporation | Method and apparatus for preparing cytological specimens |
| WO2013125959A1 (en) * | 2012-02-24 | 2013-08-29 | Instrunor As | System, apparatuses and devices for pretreating cells |
| CN110631879A (en) * | 2019-10-28 | 2019-12-31 | 宁波美生医疗器材有限公司 | Medical equipment for automatic liquid-based cell flaking and dyeing |
| CN110940568A (en) * | 2019-12-17 | 2020-03-31 | 武汉友芝友医疗科技股份有限公司 | Integrated system for cell enrichment, separation, dyeing and flaking |
| CN111551756A (en) * | 2020-04-01 | 2020-08-18 | 郑州中普医疗器械有限公司 | Full-automatic film-making dyeing scanning system |
| CN112113818A (en) * | 2020-10-09 | 2020-12-22 | 嘉兴晶铸生物科技有限公司 | Constant-temperature full-automatic cell slide-making integrated device and slide-making method |
| CN112179739A (en) * | 2020-10-09 | 2021-01-05 | 深路医学科技(武汉)有限公司 | Cell film-making device |
| CN113702143A (en) * | 2021-09-13 | 2021-11-26 | 安徽九陆生物科技有限公司 | Full-automatic liquid-based cell slice-making dyeing machine and slice-making dyeing method |
| WO2022073335A1 (en) * | 2020-10-09 | 2022-04-14 | 嘉兴晶铸生物科技有限公司 | Fully automated method for production of exfoliated cells |
| CN216847140U (en) * | 2021-09-13 | 2022-06-28 | 安徽九陆生物科技有限公司 | Full-automatic liquid-based cell slice-making dyeing machine |
| CN115338635A (en) * | 2022-10-19 | 2022-11-15 | 成都川哈工机器人及智能装备产业技术研究院有限公司 | Robot automatic assembly device and automatic assembly process for precise plug connector |
| CN115583503A (en) * | 2022-08-31 | 2023-01-10 | 深路医学科技(武汉)有限公司 | Full-automatic liquid-based cell slice staining and sealing system and control method thereof |
| WO2023109914A1 (en) * | 2021-12-15 | 2023-06-22 | 深圳市瑞图生物技术有限公司 | Liquid-based slide preparation device and slide preparation method of sample |
| CN116793799A (en) * | 2023-07-17 | 2023-09-22 | 北京华伊智能医疗科技有限公司 | Full-automatic cell slice-making, dyeing and slice-sealing integrated device |
| CN220136791U (en) * | 2023-06-30 | 2023-12-05 | 南通艾维塔生物科技有限公司 | Liquid-based thin-layer cell slice-making and dyeing integrated machine |
| CN117330373A (en) * | 2023-09-27 | 2024-01-02 | 北京华伊智能医疗科技有限公司 | Full-function automatic cell processing and sealing device and method |
-
2024
- 2024-01-24 CN CN202410095591.9A patent/CN117606885B/en active Active
Patent Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2301551A1 (en) * | 1997-08-25 | 1999-03-04 | Monogen, Inc. | Method and apparatus for automatically forming monolayers from particulate matter separated from fluid samples |
| AU2006201456A1 (en) * | 2000-03-08 | 2006-04-27 | Cytyc Corporation | Method and apparatus for preparing cytological specimens |
| WO2003045560A2 (en) * | 2001-11-26 | 2003-06-05 | Lab Vision Corporation | Automated tissue staining system and reagent container |
| WO2013125959A1 (en) * | 2012-02-24 | 2013-08-29 | Instrunor As | System, apparatuses and devices for pretreating cells |
| CN110631879A (en) * | 2019-10-28 | 2019-12-31 | 宁波美生医疗器材有限公司 | Medical equipment for automatic liquid-based cell flaking and dyeing |
| CN110940568A (en) * | 2019-12-17 | 2020-03-31 | 武汉友芝友医疗科技股份有限公司 | Integrated system for cell enrichment, separation, dyeing and flaking |
| CN111551756A (en) * | 2020-04-01 | 2020-08-18 | 郑州中普医疗器械有限公司 | Full-automatic film-making dyeing scanning system |
| CN112179739A (en) * | 2020-10-09 | 2021-01-05 | 深路医学科技(武汉)有限公司 | Cell film-making device |
| CN112113818A (en) * | 2020-10-09 | 2020-12-22 | 嘉兴晶铸生物科技有限公司 | Constant-temperature full-automatic cell slide-making integrated device and slide-making method |
| WO2022073335A1 (en) * | 2020-10-09 | 2022-04-14 | 嘉兴晶铸生物科技有限公司 | Fully automated method for production of exfoliated cells |
| WO2022073332A1 (en) * | 2020-10-09 | 2022-04-14 | 嘉兴晶铸生物科技有限公司 | Integrated device for constant-temperature fully-automatic cell slide preparation, and slide preparation method |
| CN113702143A (en) * | 2021-09-13 | 2021-11-26 | 安徽九陆生物科技有限公司 | Full-automatic liquid-based cell slice-making dyeing machine and slice-making dyeing method |
| CN216847140U (en) * | 2021-09-13 | 2022-06-28 | 安徽九陆生物科技有限公司 | Full-automatic liquid-based cell slice-making dyeing machine |
| WO2023109914A1 (en) * | 2021-12-15 | 2023-06-22 | 深圳市瑞图生物技术有限公司 | Liquid-based slide preparation device and slide preparation method of sample |
| CN115583503A (en) * | 2022-08-31 | 2023-01-10 | 深路医学科技(武汉)有限公司 | Full-automatic liquid-based cell slice staining and sealing system and control method thereof |
| CN115338635A (en) * | 2022-10-19 | 2022-11-15 | 成都川哈工机器人及智能装备产业技术研究院有限公司 | Robot automatic assembly device and automatic assembly process for precise plug connector |
| CN220136791U (en) * | 2023-06-30 | 2023-12-05 | 南通艾维塔生物科技有限公司 | Liquid-based thin-layer cell slice-making and dyeing integrated machine |
| CN116793799A (en) * | 2023-07-17 | 2023-09-22 | 北京华伊智能医疗科技有限公司 | Full-automatic cell slice-making, dyeing and slice-sealing integrated device |
| CN117330373A (en) * | 2023-09-27 | 2024-01-02 | 北京华伊智能医疗科技有限公司 | Full-function automatic cell processing and sealing device and method |
Non-Patent Citations (1)
| Title |
|---|
| LBP液基细胞学沉降式制片技术在痰脱落细胞诊断中的应用;黄勋福等;现代医院;20090715(第07期);29-31 * |
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| CN117606885A (en) | 2024-02-27 |
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