CN116930486A - Microfluidic chip and method for joint inspection of immune project - Google Patents
Microfluidic chip and method for joint inspection of immune project Download PDFInfo
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- CN116930486A CN116930486A CN202210317135.5A CN202210317135A CN116930486A CN 116930486 A CN116930486 A CN 116930486A CN 202210317135 A CN202210317135 A CN 202210317135A CN 116930486 A CN116930486 A CN 116930486A
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- 238000007689 inspection Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 10
- 239000003085 diluting agent Substances 0.000 claims abstract description 60
- 239000000758 substrate Substances 0.000 claims abstract description 44
- 238000011534 incubation Methods 0.000 claims abstract description 39
- 238000002156 mixing Methods 0.000 claims abstract description 39
- 238000000926 separation method Methods 0.000 claims abstract description 32
- 238000011068 loading method Methods 0.000 claims abstract description 24
- 210000000601 blood cell Anatomy 0.000 claims abstract description 23
- 238000012360 testing method Methods 0.000 claims abstract description 21
- 210000002966 serum Anatomy 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims description 37
- 239000002699 waste material Substances 0.000 claims description 29
- 238000001514 detection method Methods 0.000 claims description 16
- 230000007704 transition Effects 0.000 claims description 13
- 230000009471 action Effects 0.000 claims description 11
- 230000001900 immune effect Effects 0.000 claims description 11
- 238000004891 communication Methods 0.000 claims description 9
- 238000011002 quantification Methods 0.000 claims description 8
- 230000001133 acceleration Effects 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
- 210000001503 joint Anatomy 0.000 claims description 4
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims 1
- 238000004806 packaging method and process Methods 0.000 abstract description 4
- 238000012545 processing Methods 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 110
- 238000005516 engineering process Methods 0.000 description 7
- 238000003745 diagnosis Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000007599 discharging Methods 0.000 description 2
- 238000003317 immunochromatography Methods 0.000 description 2
- 238000012123 point-of-care testing Methods 0.000 description 2
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
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Abstract
The application provides a microfluidic chip and a method for joint inspection of immune projects, wherein the structure comprises a chip substrate layer; a sample loading groove and a plurality of sample incubation grooves are formed in the chip substrate layer, and an upper chip cover plate and a lower chip cover plate are respectively bonded and packaged on two sides of the sample loading groove and the plurality of sample incubation grooves; the plurality of sample incubation grooves are distributed at the outer edge of the chip substrate layer along the circumferential direction and are communicated with the sample adding groove through the mixing groove; the sample adding groove is positioned in the middle of the chip substrate layer and communicated with the mixing groove through the separation component and is used for separating blood cells from serum; the mixing tank is also communicated with the diluent storage tank and is used for mixing serum and diluent; the sample incubation groove is also communicated with the test paper placing groove; the test paper standing groove is located the chip substrate layer and is kept away from the one side of sample incubation groove. According to the technical scheme provided by the embodiment of the application, the chip is manufactured by the three-layer structure of the chip substrate layer, the chip upper cover plate and the chip lower cover plate, and the chip can be manufactured by bonding and packaging only twice, so that the processing difficulty is low.
Description
Technical Field
The application relates to the technical field of microfluidic detection equipment, in particular to a microfluidic chip and a microfluidic method for joint inspection of immune projects.
Background
The micro-fluidic chip technology is a new technology for accurately manipulating and controlling nano-liter and pico-liter fluid (biological sample fluid) in a micrometer-scale runner, and by using the technology, basic operation units such as sample preparation, reaction, separation, detection, cell culture, separation, cracking and the like related in the fields of chemistry, biology and the like can be integrated or basically integrated on a chip with a few square centimeters (even smaller), and a network is formed by the micro-runner so as to control the fluid to penetrate through the whole system and replace a technical platform with various functions of a conventional chemistry or biology laboratory. The basic characteristics and the greatest advantages of the microfluidic chip laboratory are that a plurality of unit technologies are flexibly combined and integrated on a small platform with controllable whole.
Point-of-care testing (POCT), also known as on-site rapid testing, refers to a testing method which is performed on the sampling site and rapidly obtains the testing result by using a portable analytical instrument and a matched testing reagent, and is also a main technical outlet of the micro-fluidic chip technology. At present, the detection system based on the immunochromatography technology is most widely applied in the field of instant diagnosis, but because of the large number of diagnosis detection items, each person detection item needs to be independently subjected to blood sampling detection, a large number of detection operations need to be repeated for the detection of multiple items, and a large amount of treatment time is wasted for some emergency patients, so that the gold diagnosis period of the patients is influenced. Therefore, the mode of multi-immunity project joint inspection can be realized by one sample adding, and the sample pretreatment is automatically finished on the detection chip, so that errors and interference caused by manual operation are avoided, and the method becomes an effective means for solving the clinical detection pain point of the immunochromatography technology.
Disclosure of Invention
In view of the foregoing drawbacks or shortcomings of the prior art, it is desirable to provide a microfluidic chip and method for joint inspection of immunological items.
In a first aspect, the present application provides a microfluidic chip for joint inspection of immunological items, comprising a chip substrate layer; the chip substrate layer is provided with a sample loading groove and a plurality of through sample incubation grooves, and the two sides of the chip substrate layer are respectively bonded and packaged with an upper chip cover plate and a lower chip cover plate; the plurality of sample incubation grooves are distributed at the outer edge of the chip substrate layer along the circumferential direction and are communicated with the sample loading groove through the mixing groove; the sample adding groove is positioned in the middle of the chip substrate layer and communicated with the mixing groove through a separation component, and is used for separating blood cells from serum; the mixing tank is also communicated with a diluent storage tank and is used for mixing the serum and the diluent; the sample incubation groove is also communicated with the test paper placing groove; the test paper placing groove is positioned on one side of the chip substrate layer far away from the sample incubation groove.
Further, the separation assembly comprises a blood cell separation tank and a sample quantifying tank which are communicated with each other; the blood cell separation groove is positioned at one side of the sample quantifying groove far away from the sample loading groove; the sample quantifying groove is connected with the sample adding groove through a first annular groove; the first annular groove is positioned at one side of the sample loading groove, and a first vent hole is formed in one end, far away from the sample quantifying groove; two opposite interfaces are arranged on the sample quantifying groove corresponding to the first annular groove; the sample loading groove is connected with the first annular groove through a first transition groove.
Further, the first annular groove is also communicated with the mixing groove; the mixing tank is provided with an exhaust port corresponding to the first annular groove and a liquid inlet corresponding to the separation assembly; the liquid inlet is connected between the blood cell separation groove and the sample quantifying groove through a micro-channel.
Further, the device also comprises a sample waste liquid tank; the sample waste liquid tank is positioned at one side of the blood cell separation tank and is connected with the sample quantifying tank through a second annular groove; the second annular groove is positioned at one side of the sample quantifying groove, and a second vent hole is arranged at one side of the second annular groove far away from the sample waste liquid groove; the sample quantifying groove is connected with the second annular groove through a second transition groove; and two opposite interfaces are arranged on the sample waste liquid tank corresponding to the second annular groove.
Further, the diluent storage tank is positioned in the middle of the chip substrate layer, and a diluent quantifying tank is also connected between the diluent storage tank and the mixing tank; the diluent quantifying groove is also communicated with the diluent waste liquid groove and is used for recycling the redundant diluent.
Further, the diluent waste liquid tank is connected with the diluent quantitative tank through a third annular groove; the third annular groove is positioned at one side of the diluent quantitative groove, and a third vent hole is arranged at one end of the third annular groove, which is far away from the diluent waste liquid groove; the diluent quantitative groove is connected with the third annular groove through a third transition groove; and two opposite interfaces are arranged on the diluent waste liquid tank corresponding to the third annular groove.
Further, the device also comprises an annular runner; the annular flow channel is coaxial with the chip substrate layer and is positioned at one side of the sample incubation groove close to the sample adding groove, and communication ports are respectively arranged corresponding to the sample incubation groove and used for communicating the sample incubation groove and the mixing groove.
Further, the chip fixing device also comprises a chip fixing clamping groove; the chip fixing clamping groove is positioned in the middle of the chip substrate layer and is used for being in butt joint with an external rotating piece.
In a second aspect, the present application provides a method for using a microfluidic chip for joint inspection of immune projects, comprising the steps of:
s100, mounting the chip on a rotary platform;
s110, adding a sample into the sample adding groove;
s200, starting the rotary platform, injecting a sample into the separation assembly by utilizing centrifugal force, and separating blood cells and serum in the sample;
s210, closing the rotary platform, and opening the corresponding micro-channels through capillary action;
s300, restarting the rotary platform, and enabling the serum and the diluent to enter the mixing tank respectively through centrifugal force by uniformly rotating; then, a circulation operation mode of rapid acceleration, full deceleration is carried out, so that the mixed sample in the mixing tank is oscillated, and dilution is accelerated;
s310, closing the rotary platform, and opening the corresponding micro-channels through capillary action;
s400, restarting the rotary platform, and enabling the mixed sample to enter each sample incubation groove through centrifugal force by uniformly rotating; then entering a rapid acceleration full deceleration circulating operation mode to accelerate the reaction of the mixed sample and the freeze-drying reagent in the sample incubation groove;
s410, closing the rotary platform, and opening the corresponding micro-channels through capillary action;
s420, restarting the rotary platform, and enabling the liquid in the sample incubation groove to enter the test paper placing groove by utilizing centrifugal force;
s500, detection is completed.
The application has the advantages and positive effects that:
the technical scheme is that the chip is formed by a chip substrate layer, a chip upper cover plate and a chip lower cover plate, and the chip can be manufactured by bonding and packaging for two times, so that the processing difficulty is low, and the processing cost is low; meanwhile, a plurality of sample incubation grooves are further formed in the chip substrate layer around the circumferential direction, and samples in the sample adding grooves can be respectively sent into the sample incubation grooves by matching with centrifugal force, so that the requirements of multiple detection are met; the sample can first enter the separation assembly before entering the sample incubation groove, so that blood cells and serum are separated by utilizing centrifugal force, and then, the serum and the diluent can be automatically mixed by utilizing capillary action, so that the mixed liquid required by detection is obtained.
Drawings
Fig. 1 is a schematic structural diagram of a microfluidic chip for joint inspection of immune projects according to an embodiment of the present application;
fig. 2 is a schematic structural diagram of a chip substrate layer of a microfluidic chip for joint inspection of immune projects according to an embodiment of the present application;
fig. 3 is a schematic structural diagram of a test paper placement groove of a microfluidic chip for joint inspection of immune projects according to an embodiment of the present application.
The text labels in the figures are expressed as: 100-a chip substrate layer; 101-a chip fixing clamping groove; 110-sample loading slot; 111-a blood cell separation tank; 112-sample quantification slot; 113-a first vent; 114-sample waste; 115-a second vent; 120-sample incubation slot; 121-an annular flow channel; 130-a mixing tank; 140-a diluent storage tank; 141-a diluent quantifying tank; 142-a diluent waste tank; 143-third vent holes; 150-a test paper placing groove; 200-a chip upper cover plate; 300-under-chip cover plate.
Detailed Description
In order that those skilled in the art may better understand the technical solutions of the present application, the following detailed description of the present application with reference to the accompanying drawings is provided for exemplary and explanatory purposes only and should not be construed as limiting the scope of the present application.
Referring to fig. 1-3, the present embodiment provides a microfluidic chip for joint inspection of immune projects, which includes a chip substrate layer 100; the chip substrate layer 100 is provided with a sample loading groove 110 and a plurality of through sample incubation grooves 120; the plurality of sample incubation grooves 120 are circumferentially arranged at the outer edge of the chip substrate layer 100 and are connected with the sample loading groove 110 through the mixing groove 130; the mixing tank 130 is located between the sample loading tank 110 and the sample incubation tank 120, and is also in communication with the diluent storage tank 140 for mixing the sample and the diluent.
In a preferred embodiment, the sample loading slot 110 is located in the middle of the chip substrate layer 100 and is connected to the mixing slot 130 through a separation assembly, and the sample in the sample loading slot 110 will first enter the separation assembly by centrifugal force to separate blood cells from serum, so that the serum enters the mixing slot 130 by capillary action to be mixed with the diluent.
Preferably, the separation assembly includes a blood cell separation tank 111 and a sample quantification tank 112 in communication with each other; the blood cell separation well 111 is located on a side of the sample quantification well 112 away from the sample application well 110, and is connected to the sample quantification well 112 through a micro flow channel.
Preferably, the sample quantifying groove 112 is connected with the sample loading groove 110 through a first annular groove; the first annular groove is positioned at one side of the sample loading groove 110, and one end far away from the sample quantifying groove 112 is provided with a first vent hole 113; two butt joints are arranged on the sample quantifying groove 112 corresponding to the first annular groove, and the two butt joints are respectively connected at one end of the first annular groove far away from the first vent hole 113 and are respectively used for feeding liquid into and discharging gas from the sample quantifying groove 112.
Preferably, the sample loading groove 110 is connected with the first annular groove through a first transition groove; the depth of the first transition groove is relatively smaller than that of the sample loading groove 110, and the connection position of the first transition groove and the first annular groove is positioned at one side of the first vent hole 113 away from the center of the chip substrate layer 100, so that the sample in the sample loading groove 110 can enter the sample quantifying groove 112 through the first annular groove by using centrifugal force.
In a preferred embodiment, the sample quantification reservoir 112 is also connected to a sample waste reservoir 114 for collecting excess sample; the sample waste liquid tank 114 is located at one side of the blood cell separation tank 111 at a distance from the center of the chip substrate layer 100 relatively greater than the distance of the blood cell separation tank 111 from the center of the chip substrate layer 100.
Preferably, the sample waste liquid tank 114 is connected with the sample quantifying tank 112 through a second annular groove; the second annular groove is located on the side of the sample waste liquid groove 114 near the center of the chip substrate layer 100 and, at the same time, on the side of the sample quantifying groove 112.
Preferably, a second vent hole 115 is provided at a corner of the second annular groove located away from the sample waste liquid tank 114 and from the sample quantitative tank 112; two interfaces are arranged on the sample waste liquid tank 114 corresponding to the second annular groove, and the two interfaces are respectively arranged on one side of the second annular groove far away from the second vent hole 115 and are respectively used for injecting sample liquid into the sample waste liquid tank 114 and discharging internal gas.
Preferably, the sample quantifying groove 112 is connected with the second annular groove through a second transition groove; the second transition groove has a depth relatively smaller than that of the sample quantification groove 112, and is located at an end of the sample quantification groove 112 remote from the blood cell separation groove 111.
In a preferred embodiment, the chip substrate layer 100 is further provided with a chip fixing slot 101; the chip fixing slot 101 is located in the middle of the chip substrate layer 100 and is coaxial with the chip substrate layer 100 for docking with the rotating platform.
In a preferred embodiment, the diluent storage groove 140 is disposed around the chip fixing card groove 101, and a diluent pre-packaging water box is installed inside; the diluent pre-packaging water box is opened through a release structure on the rotary platform motor, so that the diluent is released, and the structure adopts a related structure described in patent CN201910313138. X.
Preferably, the diluent storage groove 140 is connected with the mixing groove 130 through the diluent quantifying groove 141; the diluent metering groove 141 is arc-shaped and is positioned between the diluent storage groove 140 and the mixing groove 130; the diluent metering tank 141 is also in communication with the diluent waste tank 142, and may collect excess diluent to ensure that a specified amount of diluent enters the mixing tank 130.
Preferably, the diluent waste liquid tank 142 is connected with the diluent quantitative tank 141 through a third annular groove; the third annular groove is provided at one side of the diluent metering groove 141 and at one end remote from the diluent waste liquid groove 142 with a third vent 143.
Preferably, the diluent metering groove 141 is connected with the third annular groove through a third transition groove; the third transition groove has a depth relatively smaller than that of the diluent metering groove 141 and is located at one end of the diluent metering groove 141.
Preferably, the distance between the diluent waste liquid groove 142 and the chip substrate layer 100 is relatively greater than the distance between the third annular groove and the chip substrate layer 100, and two opposite interfaces are arranged corresponding to the third annular groove; the two pairs of interfaces are respectively arranged at one end of the third annular groove which is relatively far away from the third air vent 143.
In a preferred embodiment, the mixing tank 130 is provided with an air outlet and a liquid inlet corresponding to the first annular groove and the separation assembly respectively; the exhaust port is connected to the first annular groove for exhausting the gas in the mixing tank 130 through the first vent hole 113; the liquid inlet is connected between the blood cell separation tank 111 and the sample quantifying tank 112 through a micro flow channel.
In a preferred embodiment, the plurality of sample incubation grooves 120 are respectively connected to the annular flow channel 121, and the annular flow channel 121 is located between the mixing groove 130 and the sample incubation groove 120 and is in communication with the mixing groove 130, so that the sample liquid can be respectively fed into each sample incubation groove 120 by using centrifugal force.
In a preferred embodiment, the sample presentation well 120 is also in communication with a test strip placement well 150, the test strip placement well 150 being located on a side of the chip substrate layer 100 remote from the sample incubation well 120.
Preferably, the test strip includes a bottom plate that mates with the test strip placement slot 150; an NC film is arranged in the middle of the bottom plate, and a sample pad and a water absorbing paper pad for fixing the NC film are respectively arranged at two ends of the bottom plate; when the test paper is mounted in the test paper placement groove 150, the absorbent paper pad is positioned at one end near the center of the chip substrate layer 100, and the sample pad is positioned at one end far from the center.
In a preferred embodiment, both sides of the chip substrate layer 100 are respectively bonded and packaged with an upper chip cover plate 200 and a lower chip cover plate 300; meanwhile, the upper chip cover plate 200 and the lower chip cover plate 300 are provided with matching butt holes corresponding to the chip substrate layer 100 according to requirements.
In a second aspect, the present application provides a method for using a microfluidic chip for joint inspection of immune projects, comprising the steps of:
s100, mounting a chip on a rotary platform;
s110, adding a sample into the sample adding groove 110;
s200, starting a rotary platform, injecting a sample into a separation assembly by utilizing centrifugal force, and separating blood cells and serum in the sample;
s210, closing the rotary platform, and opening the corresponding micro-channels through capillary action;
s300, restarting the rotary platform, firstly rotating at a constant speed, and respectively enabling the serum and the diluent to enter the mixing tank 130 through centrifugal force; then, a circulation operation mode of rapid acceleration, full deceleration is carried out, so that the mixed sample in the mixing tank 130 is oscillated, and dilution is accelerated;
s310, closing the rotary platform, and opening the corresponding micro-channels through capillary action;
s400, restarting the rotary platform, and enabling the mixed sample to enter each sample incubation groove 120 through centrifugal force by uniformly rotating; then enters a rapid acceleration full deceleration circulating operation mode to accelerate the reaction of the mixed sample and the freeze-drying reagent in the sample incubation groove 120;
s410, closing the rotary platform, and opening the corresponding micro-channels through capillary action;
s420, restarting the rotary platform, and enabling the liquid in the sample incubation groove 120 to enter the test paper placing groove 150 by utilizing centrifugal force;
s500, detection is completed.
The principles and embodiments of the present application have been described herein with reference to specific examples, the description of which is intended only to facilitate an understanding of the method of the present application and its core ideas. The foregoing is merely illustrative of the preferred embodiments of this application, and it is noted that there is objectively no limit to the specific structure disclosed herein, since numerous modifications, adaptations and variations can be made by those skilled in the art without departing from the principles of the application, and the above-described features can be combined in any suitable manner; such modifications, variations and combinations, or the direct application of the inventive concepts and aspects to other applications without modification, are contemplated as falling within the scope of the present application.
Claims (9)
1. A microfluidic chip for joint inspection of immunological items, characterized by comprising a chip substrate layer (100); a sample loading groove (110) and a plurality of through sample incubation grooves (120) are formed in the chip substrate layer (100), and an upper chip cover plate (200) and a lower chip cover plate (300) are respectively bonded and packaged on two sides of the sample loading groove; the plurality of sample incubation grooves (120) are distributed at the outer edge of the chip substrate layer (100) along the circumferential direction and are communicated with the sample loading groove (110) through a mixing groove (130); the sample adding groove (110) is positioned in the middle of the chip substrate layer (100) and communicated with the mixing groove (130) through a separation component, and is used for separating blood cells from serum; the mixing tank (130) is also communicated with a diluent storage tank (140) for mixing the serum and diluent; the sample incubation groove (120) is also communicated with the test paper placing groove (150); the test paper placing groove (150) is positioned on one side of the chip substrate layer (100) far away from the sample incubation groove (120).
2. Microfluidic chip for joint inspection of immunological items according to claim 1, characterized in that the separation assembly comprises a blood cell separation tank (111) and a sample quantification tank (112) in communication with each other; the blood cell separation tank (111) is positioned at one side of the sample quantifying tank (112) away from the sample loading tank (110); the sample quantifying groove (112) is connected with the sample adding groove (110) through a first annular groove; the first annular groove is positioned at one side of the sample loading groove (110), and a first vent hole (113) is arranged at one end far away from the sample quantifying groove (112); two opposite interfaces are arranged on the sample quantifying groove (112) corresponding to the first annular groove; the sample loading groove (110) is connected with the first annular groove through a first transition groove.
3. The microfluidic chip for joint inspection of immunological items according to claim 2, characterized in that the first annular groove is also in communication with the mixing groove (130); an exhaust port is arranged on the mixing tank (130) corresponding to the first annular groove, and a liquid inlet is arranged corresponding to the separation assembly; the liquid inlet is connected between the blood cell separation groove (111) and the sample quantifying groove (112) through a micro-channel.
4. The microfluidic chip for joint inspection of immunological items according to claim 2, further comprising a sample waste tank (114); the sample waste liquid tank (114) is positioned at one side of the blood cell separation tank (111) and is connected with the sample quantifying tank (112) through a second annular groove; the second annular groove is positioned at one side of the sample quantifying groove (112), and a second ventilation hole (115) is arranged at one side far away from the sample waste liquid groove (114); the sample quantifying groove (112) is connected with the second annular groove through a second transition groove; two opposite interfaces are arranged on the sample waste liquid tank (114) corresponding to the second annular groove.
5. The microfluidic chip for joint inspection of immunological items according to claim 1, characterized in that the diluent storage tank (140) is located in the middle of the chip substrate layer (100), and a diluent quantifying tank (141) is further connected between the diluent storage tank and the mixing tank (130); the diluent quantifying tank (141) is also communicated with the diluent waste liquid tank (142) for recovering the redundant diluent.
6. The microfluidic chip for combined detection of immunological items according to claim 5, wherein the diluent waste liquid tank (142) is connected with the diluent quantitative tank (141) through a third annular groove; the third annular groove is positioned at one side of the diluent quantifying groove (141), and a third vent hole (143) is arranged at one end far away from the diluent waste liquid groove (142); the diluent quantitative groove (141) is connected with the third annular groove through a third transition groove; two opposite interfaces are arranged on the diluent waste liquid groove (142) corresponding to the third annular groove.
7. The microfluidic chip for joint inspection of immunological items according to claim 1, further comprising an annular flow channel (121); the annular flow channel (121) is coaxial with the chip substrate layer (100), is positioned on one side of the sample incubation groove (120) close to the sample adding groove (110), and is provided with communication ports corresponding to the sample incubation groove (120) respectively, and is used for communicating the sample incubation groove (120) and the mixing groove (130).
8. The microfluidic chip for joint inspection of immunological items according to claim 1, further comprising a chip fixing slot (101); the chip fixing clamping groove (101) is positioned in the middle of the chip substrate layer (100) and is used for being in butt joint with an external rotating piece.
9. A method of using a microfluidic chip for joint inspection of immunological items according to any one of claims 1 to 8, comprising the steps of:
s100, mounting the chip on a rotary platform;
s110, adding a sample into the sample adding groove (110);
s200, starting the rotary platform, injecting a sample into the separation assembly by utilizing centrifugal force, and separating blood cells and serum in the sample;
s210, closing the rotary platform, and opening the corresponding micro-channels through capillary action;
s300, restarting the rotary platform, and enabling serum and diluent to enter the mixing tank (130) respectively through centrifugal force by uniformly rotating; then, a circulation operation mode of rapid acceleration, full deceleration is carried out, so that the mixed sample in the mixing tank (130) is oscillated, and dilution is accelerated;
s310, closing the rotary platform, and opening the corresponding micro-channels through capillary action;
s400, restarting the rotary platform, and enabling the mixed sample to enter each sample incubation groove (120) through centrifugal force by uniformly rotating; then entering a rapid acceleration full deceleration circulating operation mode to accelerate the reaction of the mixed sample and the freeze-drying reagent in the sample incubation groove (120);
s410, closing the rotary platform, and opening the corresponding micro-channels through capillary action;
s420, restarting the rotary platform, and enabling the liquid in the sample incubation groove (120) to enter the test paper placing groove (150) by utilizing centrifugal force;
s500, detection is completed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210317135.5A CN116930486A (en) | 2022-03-29 | 2022-03-29 | Microfluidic chip and method for joint inspection of immune project |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210317135.5A CN116930486A (en) | 2022-03-29 | 2022-03-29 | Microfluidic chip and method for joint inspection of immune project |
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| Publication Number | Publication Date |
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| CN116930486A true CN116930486A (en) | 2023-10-24 |
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| CN202210317135.5A Pending CN116930486A (en) | 2022-03-29 | 2022-03-29 | Microfluidic chip and method for joint inspection of immune project |
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| Country | Link |
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| CN (1) | CN116930486A (en) |
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