CN116477983A - Composition for improving soybean yield and application - Google Patents
Composition for improving soybean yield and application Download PDFInfo
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- CN116477983A CN116477983A CN202310433368.6A CN202310433368A CN116477983A CN 116477983 A CN116477983 A CN 116477983A CN 202310433368 A CN202310433368 A CN 202310433368A CN 116477983 A CN116477983 A CN 116477983A
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- 244000068988 Glycine max Species 0.000 title claims abstract description 165
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- AEIJVYPRKAWFPN-UHFFFAOYSA-N 2-(diethylamino)ethyl hexanoate Chemical compound CCCCCC(=O)OCCN(CC)CC AEIJVYPRKAWFPN-UHFFFAOYSA-N 0.000 claims abstract description 23
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 21
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- 238000000034 method Methods 0.000 claims description 21
- 229930192334 Auxin Natural products 0.000 claims description 19
- 239000002363 auxin Substances 0.000 claims description 19
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- 102100024633 Carbonic anhydrase 2 Human genes 0.000 description 1
- 101710144958 Cyclin-D3-1 Proteins 0.000 description 1
- 101100080807 Drosophila melanogaster mt:ND2 gene Proteins 0.000 description 1
- 102000016901 Glutamate dehydrogenase Human genes 0.000 description 1
- 101710095129 Granule-bound starch synthase 1 Proteins 0.000 description 1
- 101150016680 MT-ND2 gene Proteins 0.000 description 1
- 102100028488 NADH-ubiquinone oxidoreductase chain 2 Human genes 0.000 description 1
- 101150102231 ND2 gene Proteins 0.000 description 1
- 101710108533 Serine/threonine-protein kinase BSK2 Proteins 0.000 description 1
- 108010073771 Soybean Proteins Proteins 0.000 description 1
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- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- -1 potassium sulfate compound Chemical class 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
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- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/40—Fabaceae, e.g. beans or peas
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a composition for improving soybean yield and application thereof. The technical problem to be solved is how to improve the yield of plant fruits, especially soybean. Specifically disclosed are compositions comprising urea and DA-6 and the use of the compositions for increasing plant fruit yield or for preparing a product for increasing plant fruit yield, in particular 22.5 g.hm ‑2 DA-6 and 3kg hm ‑2 The combination of urea is applied to soybean leaf surfaces in the early flowering stage and the grain-swelling stage, so that the net photosynthetic rate, the stomatal conductance degree, the number of effective pods of a single plant, the number of grains of the single plant and the weight of the single plant are obviously improved; the above groupThe composition can contribute to the improvement of soybean yield.
Description
Technical Field
The invention relates in particular to a composition for improving soybean yield and application thereof.
Background
The soybean is one of important bulk oil crops in China, is an important source of high-quality vegetable oil and protein, is an indispensable high-protein feed and industrial raw material, can fix nitrogen and fertilize soil through root nodules, and is irreplaceable in modern agricultural planting structures. Meanwhile, as the demand of soybeans in China is continuously increased, the import quantity is increased year by year, the import quantity is 80% or more of domestic consumption, the development of domestic soybean production is urgently needed, and the market supply safety is ensured. The total yield of the soybeans in the country in 2021 is 1640 ten thousand tons, the imported soybean is 9652 ten thousand tons, the soybean accounts for six times of the global soybean trade amount, and the soybean total consumption in the country is 87 percent. At present, the soybean unit yield level in China is only 60% of the average world, low unit yield and instability are main factors for restricting the soybean production and development in China, and low variety yield level is also a main factor. In the past 20 years, the unit yield of soybean in China is in an ascending trend, but is far less than that of main soybean producing countries such as the United states and Brazil, and the unit yield level in 2020 only reaches 56.83% of the United states and 57.49% of Brazil. .
Poor quality is a main factor limiting the improvement of soybean production benefits in China. Improving the yield and quality of soybeans is always a core problem of soybean cultivation research, neglecting quality, simply pursuing yield is disadvantageous in market competition, and the benefit of planting soybeans is reduced by sacrificing yield to replace the improvement of soybean quality. Synergistic improvements in soybean yield and quality have become a challenge for modern agriculture. There is a serious negative correlation between soybean yield and quality, and how to realize the synergistic improvement of the soybean yield and the soybean quality is a problem to be solved. In the cultivation production of crops, the photosynthetic efficiency of the crops can be improved through chemical regulation and control technology, the processes of growth and development of plants are regulated and controlled through internal mechanisms such as hormone synthesis and metabolism and the like, and finally the purposes of increasing the yield of the crops and improving the quality of the crops are achieved, so that the search for a scientific and effective growth regulator has positive significance for the healthy development of the soybean industry in the south.
Disclosure of Invention
The technical problem to be solved by the application is how to improve soybean yield.
In order to solve the above problems, the present application provides a composition.
The composition comprises nitrogen fertilizer and DA-6.
In the above, DA-6 is diethyl aminoethyl hexanoate.
The composition may consist of only nitrogen fertilizer and DA-6.
The nitrogen fertilizer may be urea.
In the composition, the mass ratio of the urea to the DA-6 in the composition is (120-150) 1, and the mass ratio of the urea to the DA-6 is 133:1.
In the composition, the composition is a composition for improving the yield and/or quality of soybeans.
In order to solve the above problems, the present application provides a fertilizer.
The active ingredient of the fertilizer comprises the composition of claim 1 or 2.
The fertilizer is a fertilizer for improving soybean yield and/or quality.
The use of the above composition or the above compound fertilizer in any of the following:
a1 Increasing the chlorophyll content of the soybean or preparing a product for increasing the chlorophyll content of the soybean;
a2 Increasing the net photosynthetic rate of the soybean or preparing a product for increasing the net photosynthetic rate of the soybean;
a3 Improving the air pore conductivity of the soybean leaves or preparing a product for improving the air pore conductivity of the soybean;
a4 Increasing the plant height of the soybean or preparing a product with increased plant height content;
a5 Increasing the effective branch number of the soybean single plant or preparing a product for increasing the effective branch number of the soybean single plant;
a6 Increasing the number of the effective pods of the single plant of the soybean or preparing a product for increasing the number of the effective pods of the single plant of the soybean;
a7 Increasing the number of single plants of soybean or preparing a product for increasing the number of single plants of soybean;
a8 Increasing the individual grain weight of the soybean or preparing a product for increasing the individual grain weight of the soybean;
a9 Increasing soybean yield or preparing a product that increases soybean yield.
A10 Increasing the expression of the soybean nitrogen metabolism gene or preparing a product for increasing the expression of the soybean nitrogen metabolism gene;
a11 Increasing the expression of the soybean starch/sugar metabolism related gene or preparing a product for increasing the expression of the soybean starch/sugar metabolism related gene;
a12 Increasing the synthesis of soybean auxin and the expression of signal gene or preparing a product for increasing the synthesis of auxin and the expression of signal gene.
In the above, the auxin synthesis and signal gene may be one or more of Glyma.16G129600 (auxin response family protein ARG 7), glyma.15G012800 (auxin induction protein 22C), glyma.12G034500 (auxin induction protein X10A), glyma.06G279300 (auxin induction protein X15), glyma.06G281100 (auxin induction protein 6B), glyma.18G110300 (auxin response protein SAUR 71), glyma.12G124200 (auxin induction protein 6B), glyma.17G400 (serine/threonine protein kinase BSK 2), glyma.12G034400 (auxin induction protein 6B-like), glyma.17G6000 (dual element response regulator ARR 12), glyma.G400 (auxin response protein IAA 8), glyma.04G042000 (cyclin-D3-1).
In the above, the nitrogen metabolism gene may be one or more of glyma 13g084000 (nitrate reductase NADH), glyma 06g182700 (carbonic anhydrase 2), glyma 06g109200 (nitrate reductase NADH2 is induced), glyma 02g072200 (glutamate dehydrogenase).
In the above, the starch and sugar metabolism related gene may be one or several of Glyma.11G129600 (β -glucosidase 24), glyma.05G056300 (fructofuranosidase), glyma.16G018500 (granule-bound starch synthase 1), glyma.11G116600 (glucose-1-phosphoadenyltransferase large subunit 1), glyma.07G049900 (granule-bound starch synthase).
Above, the soybeans may be middle beans 63 or Anhui beans 28.
In order to solve the above problems, the present application provides a soybean planting method.
The method comprises spraying the composition or the fertilizer on the soybeans.
In the above, the soybean plant may be soybean at the drum stage or after maturation.
To address the above problems, the present application provides methods for improving soybean yield and/or quality.
The method comprises spraying the composition or the fertilizer on the soybeans.
In the above, the spraying may be foliar spraying.
In the above, the soybeans may be primary flowering or drum stage soybeans.
The concentration of the composition or the fertilizer effective substance is 3 kg.375L of urea -1 ；DA-622.5g·375L -1 。
In the method, the sprayed part is a leaf surface.
The method, wherein the amount of the application is 22.5 g.hm -2 DA-6 and 3kg hm -2 And (3) urea.
Use of the method described above in any of the following:
b1 Increasing the chlorophyll content of the soybean or preparing a product for increasing the chlorophyll content of the soybean;
b2 Increasing the net photosynthetic rate of the soybean or preparing a product for increasing the net photosynthetic rate of the soybean;
b3 Improving the air pore conductivity of the soybean leaves or preparing a product for improving the air pore conductivity of the soybean;
b4 Increasing the plant height of the soybean or preparing a product with increased plant height content;
b5 Increasing the effective branch number of the soybean single plant or preparing a product for increasing the effective branch number of the soybean single plant;
b6 Increasing the number of the effective pods of the single plant of the soybean or preparing a product for increasing the number of the effective pods of the single plant of the soybean;
b7 Increasing the number of single plants of soybean or preparing a product for increasing the number of single plants of soybean;
b8 Increasing the individual grain weight of the soybean or preparing a product for increasing the individual grain weight of the soybean;
b9 Increasing soybean yield or preparing a product that increases soybean yield.
B10 Increasing the expression of the soybean nitrogen metabolism gene or preparing a product for increasing the expression of the soybean nitrogen metabolism gene;
b11 Increasing the expression of the soybean starch/sugar metabolism related gene or preparing a product for increasing the expression of the soybean starch/sugar metabolism related gene;
b12 Increasing the synthesis of soybean auxin and the expression of signal gene or preparing a product for increasing the synthesis of auxin and the expression of signal gene.
Above, the soybeans may be middle beans 63 or Anhui beans 28.
Advantageous effects
The application discloses a composition for improving yield of southern soybeans, a preparation method and application thereof. The invention uses the combination of urea and DA-6 to soybean leaf surface, especially 22.5 g-hm -2 DA-6 and 3kg hm -2 The combination of urea can obviously improve the net photosynthetic rate, the stomatal conductance, the number of the effective pods of the single plant, the number of the single plant and the weight of the single plant of soybean, and is especially suitable for the leaf surfaces of the soybean in the early flowering stage and the grain-swelling stage
Drawings
FIG. 1 is a graph showing the effect of the combination of diethyl aminoethyl hexanoate and urea on photosynthetic characteristics of soybeans.
FIG. 2 is an effect of the combination of diethyl aminoethyl hexanoate and urea on soybean yield traits.
FIG. 3 shows gene expression in the main pathway affected by the spraying of diethyl aminoethyl hexanoate and urea.
Detailed Description
The following detailed description of the invention is provided in connection with the accompanying drawings that are presented to illustrate the invention and not to limit the scope thereof. The examples provided below are intended as guidelines for further modifications by one of ordinary skill in the art and are not to be construed as limiting the invention in any way.
The experimental methods in the following examples, unless otherwise specified, are conventional methods, and are carried out according to techniques or conditions described in the literature in the field or according to the product specifications. Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
The following examples used SPSS17.0 statistical software to process the data, and the experimental results were expressed as mean.+ -. Standard deviation, with significant differences indicated by P < 0.05 (different lower case letters) using the One-way ANOVA test.
DA-6 in the following examples is diethyl aminoethyl hexanoate with molecular formula C 12 H 25 NO 2 CAS number 10369-83-2, a product of Solarbio company D7080-25 g.
The urea in the examples described below is produced by Soxhaustao (Solarbio) company U8020-500g with CAS number 57-13-6.
Effect of example 1 composition on soybean yield
The soybean variety to be tested is a middle soybean 63 of high-yield, high-quality and stable-yield soybean variety (a variety bred by oil crop institute of national academy of agricultural sciences), and a high-quality and high-protein soybean variety Anhui soybean 28 (a variety bred by crop institute of national academy of agricultural sciences).
The test was performed at the Jinmen group forest double stele test base and the Wu Hanyang logic base. The soil type of the test land is clay, the soil quality and fertility are medium, and the topography is flat. Before sowing, applying a 14-16-15 potassium sulfate compound fertilizer 375kg & hm-2.
Planting:
the test of Vitex and Yang Luoji adopts the design of split area test, adopts the mode of sowing and planting by a wire pulling point, and has 3 times of repetition, the row length is 3 meters, the row spacing is 0.5m, the plant spacing is 0.1m,5 rows of areas and the cell area is 7.5m 2 . Sowing in 6 months and 5 days in 2020, manually dibbling, sowing 3 seeds in each hole, and ensuring full seedlings.
The following planting methods are adopted for the tests of the chaste tree doors and the Yang Luoji lands:
the planting area was equally divided into 8 parts, 1-4 area planting medium beans 63,5-8 area planting Anhui beans 28. In the 1-4 area, the 1 area is respectively sprayed with T1 liquid 375 L.hm on the leaf surface in the early flowering period (R1) and the grain-swelling period (R5) -2 Each time (T1 treatment); 2 area foliar spray composition T2 solution 375 L.hm at the early flowering stage (R1) and the graining stage (R5), respectively -2 Each time (T2 treatment); 3 area foliar spray composition T3 solution 375 L.hm at the early flowering stage (R1) and the graining stage (R5), respectively -2 Each time (T3 treatment); 4 area foliar spray composition T4 solution 375 L.hm at the early flowering stage (R1) and the grainy stage (R5), respectively -2 One each (T4 treatment). 5-8 area, 5 area is subjected to foliar spraying of composition T1Liqui375 L.hm in the early flowering phase (R1) and the grainy phase (R5), respectively -2 Each time (T1 treatment); 6 area foliar spray composition T2 solution 375 L.hm at the early flowering stage (R1) and the grainy stage (R5), respectively -2 Each time (T2 treatment); 7 area foliar spray composition T3 solution 375 L.hm at the early flowering stage (R1) and the grainy stage (R5), respectively -2 Each time (T3 treatment); 8 area foliar spray composition T4 solution 375 L.hm at the early flowering stage (R1) and the grainy stage (R5), respectively -2 One each (T4 treatment).
T1 is water; t2 is urea; t3 is DA-6; composition T4: the mass ratio of DA-6 to urea is 3000 parts by mass of urea and 2 parts by mass of DA-6.
T1 liquid: and (3) water.
Composition T2 solution: dissolving T2 in water to make urea concentration reach 3kg.375L -1 A solution of composition T2 was obtained.
Composition T3 solution: dissolving T3 in water to reach DA-6 concentration of 22.5g.375L -1 A solution of composition T3 was obtained.
Composition T4 solution: dissolving composition T4 in water to obtain composition T4 solution, wherein urea content in composition T4 solution is 3kg.375L -1 DA-6 content of 22.5 g.375L -1 A solution of composition T4 was obtained. In the solution of the composition T4, the mass ratio of the urea to the DA-6 is 133:1.
And 9, the seeds are ripe successively in the late 9 th month, harvested and manually harvested.
The measurement items and methods:
(1) Effect of composition on gene expression:
the two bases of Jinmen and yang can respectively operate the soybean in the 8 regions: the method comprises the steps of taking soybean three-leaf-inverted leaves 20 days after flowering (after primary flowering (R1) is completed) of middle beans 63 treated by a T4 method, carrying out expression profile measurement, taking 5 mixed samples, taking T1 treatment as a control, carrying out standardized treatment on the count number of each sample gene by using DESeq software (using BaseMean values to estimate the expression quantity), respectively taking T1 treatment in each place as a control, calculating difference multiples, carrying out difference significance test on reads numbers by using NB (negative binomial distribution test), and finally screening differential protein coding genes according to the difference multiples and the difference significance test results.
As a result of analysis, it was found that the soybeans of the 1 region of the Vitex and the Yangguan bases treated with T4 had up-regulated expression in Glyma.13G084000, glyma.06G182700, glyma.06G109200 and Glyma.02G072200 in nitrogen metabolic pathway. Glyma.11g129600, glyma.05g056300, glyma.16g018500, glyma.11g116600 and glyma.07g049900 up-regulate expression in the starch and sugar metabolic pathways.
The glama.16g129600, glama.15g012800, glama.12g034500, glama.06g279300, glama.06g281100, glama.18g110300, glama.12g124200, glama.17g153400, glama.12g034400, glama.17g076000, glama.14g185400 and glama.04g042000 of the auxin synthesis and signaling pathway. Referring to soybean reference genome database soybase for related gene sequence informationhttps://www.soybase.org/)。
(2) Metabolic detection:
the two bases of Jinmen and yang can respectively operate the soybean in the 8 regions: after finishing the leaf surface spraying in the grain-swelling period (R5), selecting 8:00-11:00 am on sunny days, respectively and continuously selecting 5 soybean materials at one time, and respectively measuring the net photosynthetic rate (Pn), the stomatal conductance (Gs), the cell gap CO2 concentration (Ci) and the transpiration rate (Tr) of each treatment functional leaf by using a Li-6800 portable photosynthetic system analyzer (Li-Cor company in the United states). And calculating the water utilization efficiency of the leaf blade according to the measured net photosynthetic rate and the measured transpiration rate, wherein the water utilization efficiency is the ratio of the net photosynthetic rate to the transpiration rate. And (3) carrying out chlorophyll content measurement by adopting a SPAD-502 portable chlorophyll meter, and measuring the inverted 3 leaves of the soybeans in the field. While samples were taken for transcriptome sequencing.
The results show that chlorophyll is the main pigment for photosynthesis of plants, and the accumulation of photosynthetic products can be promoted by increasing chlorophyll. As shown in fig. 1 and table 1, after the diethyl aminoethyl hexanoate and urea are sprayed in a compound way (T4 treatment), the chlorophyll content is improved; the high protein variety Wan bean 28 increases the most. The net photosynthetic rate of the 2 soybean varieties and the change rule of the stomatal conductance are consistent, and the net photosynthetic rate and the change rule of the stomatal conductance are all in an increasing trend, so that the effect is obvious compared with the effect of independently applying DA-6 or urea. In conclusion, the compound spraying of the diethyl aminoethyl hexanoate and the urea can effectively regulate and control the net photosynthetic rate of the soybean leaves, so that photosynthesis is enhanced, accumulation of organic matters is facilitated, and a foundation is laid for yield increase.
TABLE 1 influence of the Complex of diethyl aminoethyl hexanoate and Urea on the photosynthetic Properties of soybeans
(3) Detection of protein and fat content in the drum stage:
the two bases of Jinmen and yang can respectively operate the soybean in the 8 regions: after finishing the leaf spraying in the grain-swelling period (R5), 5 soybean materials are selected continuously at one time respectively, and the protein and fat contents of the mature grains are measured by using a Zeltex ZX50 hand-held grain analyzer in the United states, as shown in Table 2, the protein and fat contents are kept in a stable range and slightly increased, and the total amount of protein and total amount of oil in each mu can be increased because the compound nitrogen fertilizer and the diethyl aminoethyl hexanoate contribute to the increase of the soybean yield.
TABLE 2 influence of the Complex of diethyl aminoethyl hexanoate and Urea on soy protein and fat content
(4) Protein and fat content determination after maturation:
the soybean in the 8 areas is respectively processed in two places of Jinmen and Yangguang: and during maturation, 10 plants are continuously selected for indoor seed examination and quality character analysis. Protein and fat content were measured using a Zeltex ZX50 hand-held cereal analyzer in the united states. And 3 rows in the middle of the district are taken for yield measurement during harvesting. Relevant statistical analysis was performed with SPSS17.0 software, and data was calculated and plotted using Microsoft Excel 2010.
As shown in fig. 2 and table 3, after the treatment of spraying diethyl aminoethyl hexanoate and urea (T4 treatment), 10 plants were continuously selected for indoor seed test per region in the maturity period, and agronomic traits and yield traits were measured, and the results showed that the plant height and the effective branch number of individual plants of soybean varieties were increased. The yield component factors of the soybean variety are improved, and the yield is improved. The number of the effective pods of the single plants, the number of the single plants and the weight of the single plants of the medium beans 63 are increased, so that the yield is improved to be a significant level, compared with the comparison of T1 sprayed with clear water, the yield of two test points is increased by 36.6 percent and 48.4 percent respectively, and compared with the single application of urea and diethyl aminoethyl hexanoate, the yield is increased by 2 to 5 percent; the number of the effective pods of the single plant, the number of the single plant and the weight of the single plant of the high-protein Anhui bean 28 are all obviously improved, compared with a T1 sprayed clear water control, the yield of two test points is respectively increased by 34.7 percent and 41.8 percent, and compared with the independent application of urea and diethyl aminoethyl hexanoate, the yield is increased by about 4 percent. Overall evaluation shows that the compound nitrogenous fertilizer and the diethyl aminoethyl hexanoate are beneficial to the improvement of soybean yield.
The present invention is described in detail above. It will be apparent to those skilled in the art that the present invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with respect to specific embodiments, it will be appreciated that the invention may be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. The application of some of the basic features may be done in accordance with the scope of the claims that follow.
TABLE 3 influence of the Complex of diethyl aminoethyl hexanoate and Urea on soybean yield traits
Claims (10)
1. Composition, characterized in that it comprises nitrogen fertilizer and DA-6.
2. Composition according to claim 1, wherein the mass ratio of urea to DA-6 in the composition is (120-150): 1, such as the mass ratio of urea to DA-6 is 133:1.
3. Composition according to claim 1 or 2, characterized in that it is a composition for increasing the yield and/or quality of soybeans.
4. A fertilizer, characterized in that the active ingredient of the fertilizer comprises the composition of claim 1 or 2.
5. Use of a composition according to claim 1 or 2 or a compound fertilizer according to claim 3 in any of the following:
a1 Increasing the chlorophyll content of the soybean or preparing a product for increasing the chlorophyll content of the soybean;
a2 Increasing the net photosynthetic rate of the soybean or preparing a product for increasing the net photosynthetic rate of the soybean;
a3 Improving the air pore conductivity of the soybean leaves or preparing a product for improving the air pore conductivity of the soybean;
a4 Increasing the plant height of the soybean or preparing a product with increased plant height content;
a5 Increasing the effective branch number of the soybean single plant or preparing a product for increasing the effective branch number of the soybean single plant;
a6 Increasing the number of the effective pods of the single plant of the soybean or preparing a product for increasing the number of the effective pods of the single plant of the soybean;
a7 Increasing the number of single plants of soybean or preparing a product for increasing the number of single plants of soybean;
a8 Increasing the individual grain weight of the soybean or preparing a product for increasing the individual grain weight of the soybean;
a9 Increasing soybean yield or preparing a soybean yield-increasing product;
a10 Increasing the expression of the soybean nitrogen metabolism gene or preparing a product for increasing the expression of the soybean nitrogen metabolism gene;
a11 Increasing the expression of the soybean starch/sugar metabolism related gene or preparing a product for increasing the expression of the soybean starch/sugar metabolism related gene;
a12 Increasing the synthesis of soybean auxin and the expression of signal gene or preparing a product for increasing the synthesis of auxin and the expression of signal gene.
6. A method of growing soybeans, said method comprising spraying soybeans with the composition of any one of claims 1 to 3 or the fertilizer of claim 4.
7. A method for improving the yield and/or quality of soybeans, characterized in that the method comprises spraying soybeans with the composition of any one of claims 1 to 3 or the fertilizer of claim 4.
8. The method of claim 6 or 7, wherein the sprayed location is a foliar surface.
9. The method of claim 8, wherein the amount administered is 22.5 g-hm -2 DA-6 and 3kg hm -2 And (3) urea.
10. Use of the method of any one of claims 6-9 in any one of the following:
b1 Increasing the chlorophyll content of the soybean or preparing a product for increasing the chlorophyll content of the soybean;
b2 Increasing the net photosynthetic rate of the soybean or preparing a product for increasing the net photosynthetic rate of the soybean;
b3 Improving the air pore conductivity of the soybean leaves or preparing a product for improving the air pore conductivity of the soybean;
b4 Increasing the plant height of the soybean or preparing a product with increased plant height content;
b5 Increasing the effective branch number of the soybean single plant or preparing a product for increasing the effective branch number of the soybean single plant;
b6 Increasing the number of the effective pods of the single plant of the soybean or preparing a product for increasing the number of the effective pods of the single plant of the soybean;
b7 Increasing the number of single plants of soybean or preparing a product for increasing the number of single plants of soybean;
b8 Increasing the individual grain weight of the soybean or preparing a product for increasing the individual grain weight of the soybean;
b9 Increasing soybean yield or preparing a soybean yield-increasing product;
b10 Increasing the expression of the soybean nitrogen metabolism gene or preparing a product for increasing the expression of the soybean nitrogen metabolism gene;
b11 Increasing the expression of the soybean starch/sugar metabolism related gene or preparing a product for increasing the expression of the soybean starch/sugar metabolism related gene;
b12 Increasing the synthesis of soybean auxin and the expression of signal gene or preparing a product for increasing the synthesis of auxin and the expression of signal gene.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106146146A (en) * | 2016-06-29 | 2016-11-23 | 山东农业大学 | A kind of Special foliage fertilizer for wheat |
| US20190320661A1 (en) * | 2017-01-03 | 2019-10-24 | Monsanto Technology, Llc | Microbial compositions and methods |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106146146A (en) * | 2016-06-29 | 2016-11-23 | 山东农业大学 | A kind of Special foliage fertilizer for wheat |
| US20190320661A1 (en) * | 2017-01-03 | 2019-10-24 | Monsanto Technology, Llc | Microbial compositions and methods |
Non-Patent Citations (2)
| Title |
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| 杨平华: "农田植物生长调节剂使用技术", 31 January 2009, 四川科学技术出版社, pages: 180 * |
| 郝青南: "氮肥与复硝酚钠复配对南方大豆光合特性和产量及品质的影响", 中国油料作物学报, vol. 44, no. 3, 30 June 2022 (2022-06-30), pages 610 - 620 * |
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