CN116200443B - Lactobacillus plantarum and application thereof in preparation of sesame seed meal with low phytic acid, high acid protein solubility and high organic acid - Google Patents
Lactobacillus plantarum and application thereof in preparation of sesame seed meal with low phytic acid, high acid protein solubility and high organic acid Download PDFInfo
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- 235000003434 Sesamum indicum Nutrition 0.000 title claims abstract description 91
- 235000012054 meals Nutrition 0.000 title claims abstract description 87
- 239000002253 acid Substances 0.000 title claims abstract description 43
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 43
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 43
- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 37
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 37
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 37
- 229940068041 phytic acid Drugs 0.000 title claims abstract description 36
- 239000000467 phytic acid Substances 0.000 title claims abstract description 36
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 title claims abstract description 35
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 title claims abstract description 35
- 235000002949 phytic acid Nutrition 0.000 title claims abstract description 35
- 150000007524 organic acids Chemical class 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims description 11
- 244000000231 Sesamum indicum Species 0.000 title 1
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- 238000000855 fermentation Methods 0.000 claims abstract description 26
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- 238000000034 method Methods 0.000 claims abstract description 20
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 12
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- 238000001035 drying Methods 0.000 claims abstract description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 6
- 230000000694 effects Effects 0.000 claims description 43
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 26
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Abstract
The application discloses lactobacillus plantarum and application thereof in preparing sesame seed meal with low phytic acid, high acid protein and high organic acid, wherein the fermented sesame seed meal is prepared by carrying out synergistic fermentation on alkaline protease, phytase and lactobacillus plantarum enzyme and carrying out high-temperature drying and crushing, the obtained fermented enzymolysis sesame seed meal product has the degradation rate content of phytic acid of more than or equal to 65%, the acid protein content of more than or equal to 50%, the lactic acid content of more than or equal to 42g/kg, the method can improve the nutritional value of sesame seed meal feed, reduce the use amount of soybean meal in a feed formula, improve the feed digestibility, and obviously reduce the feed egg ratio and the daily average consumption of feed by replacing soybean meal with a proper amount of nitrogen in daily ration of laying hens.
Description
Technical Field
The invention belongs to the technical field of biological feeds, and particularly relates to lactobacillus plantarum and application thereof in preparation of sesame seed meal with low phytic acid, high acid protein and high organic acid.
Background
The China is the largest feed production country in the world, the feed resources are seriously deficient, the grain competition of people and livestock is increasingly serious, and especially after the corn-bean pulp feed is popularized, the high-quality protein feed resources such as bean pulp, fish meal and the like are seriously dependent on import; people interweave many factors such as the trend of good life, the transformation and upgrading requirements of the feed industry, the continuous increase of environmental protection pressure and the like, and creates a history opportunity for the development of the biological feed industry; in addition, the breakthrough progress of biotechnology, animal nutrition, feeding management, fermentation process, equipment and other multidisciplinary technology integration and fusion, and the cross-boundary fusion and collaborative research among scientists provide a new thought and technical route for developing and utilizing the miscellaneous meal protein source feed.
The sesame seed meal is a byproduct obtained after oil extraction of sesame seeds, the main component of the sesame seed meal is sesame protein, the sesame seed meal contains more than 45% of crude protein, the amino acid composition of the sesame seed meal is similar to that of soybean meal with equal protein content, and the sesame seed meal is rich in essential amino acids of various animal organisms, so the sesame seed meal is a high-nutrition plant protein resource. When the price of the bean pulp is high, sesame seed pulp is low in price and is more and more favored by feed manufacturers. However, because the lysine content is low, extra lysine is needed to be added when the feed is used so as to balance amino acid and improve the digestion utilization rate of the feed. In addition, the use of sesame seed meal in poultry is limited due to the influence of factors such as appearance, smell, and the inclusion of some anti-nutritional factors (phytic acid and oxalic acid).
In the prior art, the fermented sesame seed meal product prepared by the enzymolysis or microbial fermentation process has short fermentation period, so that the enzymolysis effect is poor, the fermentation is insufficient, the nutritive value of the sesame seed meal cannot be fully utilized, and the production process is complex and the production cost is high, thereby being unfavorable for popularization. Therefore, the preparation method for fermenting and enzymolysis sesame seed meal is needed to be solved, not only can fully utilize the nutritional value of the sesame seed meal, but also can reduce the phytic acid content of the fermenting and enzymolysis sesame seed meal, improve the acid soluble protein content and the organic acid content, and improve the feed digestibility.
Disclosure of Invention
The invention aims to provide a preparation method and application of fermented and enzymolyzed sesame seed meal, which can effectively reduce the phytic acid content in the sesame seed meal, improve the acid soluble protein and organic acid content, improve the feeding nutritive value of the sesame seed meal, reduce the using amount of soybean meal in a feed formula and improve the digestibility of the feed.
In order to achieve the above purpose, the present invention provides the following technical solutions:
A fermented sesame seed cake with low phytic acid, high acid soluble protein and high organic acid is prepared from alkaline proteinase, phytase and Lactobacillus plantarum (Lactobacillus Plantarum) BFC1602 bacteria enzyme through synergistic fermentation, high-temperature drying and pulverizing.
The Lactobacillus plantarum (Lactobacillus Plantarum) BFC1602 has been preserved in the China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.13132 at the date of 21 in the year of 10 of 2016.
Specifically, the application provides a preparation method of fermented sesame seed meal with low phytic acid, high acid protein and high organic acid, which comprises the following steps:
step 1, crushing sesame seed meal, sieving, adding alkaline protease according to the addition amount (w/w) of 0.5-2.0%, and uniformly mixing with the sieved sesame seed meal;
Step 2, uniformly mixing the mixed material in the step 1 with water according to the weight ratio of (1-3), and regulating the pH value to be 9.5-11.5;
Step 3, controlling the temperature of the materials to be 45-60 ℃ and carrying out enzymolysis for 2-8 hours, wherein the enzymolysis process is continuously stirred;
step 4, adjusting the pH value of the material subjected to enzymolysis in the step 3 to 5.5-7.0;
Step 5, the phytase is dissolved in water solution according to 0.01% -0.03% (w/w) and lactobacillus plantarum BFC1602 according to 0.1% (w/w), and the mixture is added into the material obtained in the step 4, uniformly mixed, the water content is controlled to be 50% -80%, and the temperature is controlled to be 35-45 ℃ for anaerobic fermentation for 3d;
drying the fermented material in the step 6 at high temperature until the water content is less than or equal to 12%; pulverizing, and sieving to obtain the fermented enzymolysis sesame seed meal product with low phytic acid, high acid soluble protein and high organic acid.
Further, the alkaline protease activity unit of the step 1 is 200000U/g, and the alkaline protease activity unit is added according to the adding amount of 1.0-2.0%; the phytase activity unit of the step 5 is 10000U/g, the preservation number of the lactobacillus plantarum (Lactobacillus Plantarum) BFC1602 is CGMCC No.13132, and the viable count is more than or equal to 2.0X10 10 CFU/g.
Further, the ratio of the feed to the water in the step 2 is 3:2, and the pH value is 10.5-11.5.
Further, the material temperature in the enzymolysis in the step 3 is 55-60 ℃ and the enzymolysis time is 4 hours.
Further, the water content in the step 5 is 50%, the fermentation temperature is 35 ℃ and the time is 3d.
The application also protects the fermented sesame seed meal with low phytic acid, high acid soluble protein and high organic acid, which is prepared by the production method.
The application also protects a laying hen feed prepared from the fermented sesame seed meal with low phytic acid, high acid protein and high organic acid, and specifically a ration with 5% of soybean meal replaced by nitrogen such as fermented and hydrolyzed sesame seed meal.
The application has the beneficial effects that:
1. The invention has simple production process, less investment of production equipment and low production cost. The invention can effectively reduce the phytic acid content in the sesame meal, improve the acid soluble protein and organic acid content, improve the feeding nutritive value of the sesame meal, reduce the use amount of the soybean meal in the feed formula and improve the digestibility of the feed; wherein, the degradation rate of the phytic acid of the fermented and enzymolyzed sesame seed meal prepared by the invention is more than or equal to 65%, the acid soluble protein accounts for more than or equal to 50%, and the lactic acid content is more than or equal to 42g/kg.
2. The invention adopts commercial alkaline protease and phytase to effectively reduce the anti-nutritional factors and improve the content of acid soluble protein and organic acid. The fermented and enzymolyzed sesame seed meal provided by the invention can reduce the usage amount of soybean meal, improve the utilization rate of feed, reduce the production cost and reduce environmental pollution. The proper amount of nitrogen in the daily ration of the laying hen replaces bean pulp, so that the feed-egg ratio and the daily average consumption can be obviously reduced.
3. According to the invention, an optimal reaction process for enzymolysis of sesame seed meal is obtained through a comparison test, wherein the single factor comparison shows that when the enzymolysis temperature is increased, the ratio of acid soluble protein to crude protein is slowly increased, and the enzymolysis effect is better when the enzymolysis temperature is 50-60 ℃; when the addition amount of alkaline protease is increased, the ratio of acid soluble protein to crude protein is slowly increased, and when the addition amount of alkaline protease is 1-2%, the enzymolysis effect is better; when the pH value is increased, the acid soluble protein is slowly increased to account for crude protein, and when the enzymolysis pH value is 10.5-11.5, the enzymolysis effect is good.
4. The optimal reaction conditions and the bacterial enzyme ratio of the two-step enzymolysis fermented sesame seed meal are obtained through comparative test screening. The two-step reaction test has the optimal effect, namely, the alkaline protease is used for carrying out the first-step enzymolysis on the sesame seed meal, and the phytase and the lactobacillus plantarum BFC1602 are combined with the second-step enzymolysis to ferment the sesame seed meal, so that the acid-soluble protein content, the lactic acid content and the phytic acid degradation rate are optimal. The comparison group shows that the effect of the single use of any one step of enzymolysis or fermentation of sesame seed meal is not good in combination, and the bacterial enzyme combination has a synergistic technical effect when applied to the sesame seed meal fermentation method.
5. The two enzymes are exchanged through a control group, phytase is used for enzymolysis in the first step, alkaline protease and lactobacillus plantarum are used for fermentation in the second step, the effect is obviously lower than that of the method for enzymolysis by alkaline protease, and then the method for fermenting sesame seed meal by combining the phytase and lactobacillus plantarum is described, namely, the method for fermenting sesame seed meal by combining the phytase with the lactobacillus plantarum is the best in the production of low phytic acid high acid protein-soluble high organic acid after the enzymolysis reaction of the alkaline protease.
Preservation description
Chinese name: lactobacillus plantarum
Latin name: lactobacillus Plantarum A
Strain number: BFC1602
Preservation mechanism: china general microbiological culture Collection center (China general microbiological culture Collection center) for short: CGMCC
Address: beijing, chaoyang district North Star, west Lu No. 1, 3
Preservation date: 2016, 10, 21 days
Accession numbers of the preservation center: CGMCC No.13132.
Detailed Description
The technical scheme of the present invention is further defined below in conjunction with the specific embodiments, but the scope of the claimed invention is not limited to the description.
The sesame seed meal in the embodiment of the application is commercially available, and the requirements of GB/22477-2008 sesame seed meal are met, alkaline protease and phytase are commercially available, the activity unit of the alkaline protease is 200000U/g, the activity unit of the phytase is 10000U/g, and the viable count of the lactobacillus plantarum BFC1602 is more than or equal to 2.0X10 10 CFU/g. The acid soluble protein detection method in the test process refers to: GB/T22492-20088 B.4.1; crude protein detection method reference: GB/T6432-2018
Example 1
A preparation method of fermented sesame seed meal with low phytic acid, high acid protein and high organic acid comprises the following steps:
Step 1, crushing sesame seed meal, sieving 95% of the crushed sesame seed meal by a 40-target standard sieve, adding alkaline protease according to the addition amount (w/w) of 1%, and uniformly mixing the crushed sesame seed meal with the sieved sesame seed meal;
Step 2, uniformly mixing the mixed material in the step 1 with water according to the weight ratio of 3:2, and regulating the pH value to be controlled at 10.5;
Step 3, controlling the temperature of the materials at 55 ℃ and carrying out enzymolysis for 4 hours, and continuously stirring in the enzymolysis process;
step 4, adjusting the pH value of the material subjected to enzymolysis in the step 3 to 6.0;
step 5, the phytase is dissolved in water solution according to 0.03% (w/w) and lactobacillus plantarum BFC1602 according to 0.1% (w/w), and the mixture is added into the material obtained in the step4, uniformly mixed, the water content is controlled at 50%, and the temperature is controlled at 35 ℃ for anaerobic fermentation for 3d;
Drying the fermented material in the step 6 at a high temperature until the water content is 12%; pulverizing, and sieving with 40 mesh sieve to obtain fermented and hydrolyzed sesame seed cake product with low phytic acid, high acid soluble protein and high organic acid.
Example 2
A fermented sesame seed meal with low phytic acid, high acid soluble protein and high organic acid is prepared by synergistic fermentation of alkaline protease, phytase and lactobacillus plantarum (Lactobacillus Plantarum) BFC1602 bacterial enzyme, and is prepared by high-temperature drying and crushing, and the specific preparation method steps are the same as those of example 1.
Example 3
A laying hen feed prepared from fermented sesame seed meal with low phytic acid, high acid soluble protein and high organic acid, and the specific preparation method of the specific fermented enzymolysis sesame seed meal is the same as that of example 1, and the feed is daily ration with 5% of constant nitrogen instead of soybean meal.
Test of the Effect of the enzymolysis temperature on the acid-soluble protein content in fermented sesame seed meal
Referring to steps 1-3 of example 1, only one enzymatic hydrolysis reaction was performed. And (3) uniformly mixing alkaline protease A, B and C with sesame seed meal after 40-mesh sieving according to the addition amount of 1%, wherein the material-water ratio is 3:2, and the enzymolysis temperature is only set at 45 ℃, 50 ℃, 55 ℃ and 60 ℃ respectively, and the enzymolysis is carried out for 4 hours. The alkaline protease A is a Shandong large bioengineering Co-Ltd product, the alkaline protease B is a Beijing challenge biotechnology Co-Ltd product, the alkaline protease C is a Xingtai Situte biotechnology Co-Ltd product, the enzyme activity units are 200000U/g, and each group of tests is repeated 3 times.
TABLE 1 results of acid soluble protein to crude protein ratio in fermented sesame seed meal
Analysis from table 1 shows that: under the same condition, the enzymolysis temperature is increased, the ratio of acid-soluble protein to crude protein is slowly increased, the enzymolysis effect is better when the enzymolysis temperature is 60 ℃, and the difference of the enzymolysis effect is not obvious after the temperature is increased. The difference of enzymolysis effects of different types of alkaline proteases at 55-60 ℃ is not obvious. In order to save cost, enzymolysis at 55 ℃ can be used in the industrial production process.
Test of influence of the addition amount of the dialkali protease on the acid-soluble protein content in the fermented sesame seed meal
Referring to steps 1-3 of example 1, only one enzymatic hydrolysis reaction was performed. Alkaline protease A, B and C are respectively added in the amounts of 0.5%, 1.0%, 1.5% and 2.0%, and by taking no alkaline protease as a reference, the mixture is uniformly mixed with sesame seed meal after 40-mesh sieving, the ratio of feed to water is 3:2, the pH is 9.5, and enzymolysis is carried out for 4 hours at 55 ℃. The alkaline protease A is a Shandong large bioengineering Co-Ltd product, the alkaline protease B is a Beijing challenge biotechnology Co-Ltd product, the alkaline protease C is a Xingtai Situte biotechnology Co-Ltd product, the enzyme activity units are 200000U/g, and each group of tests is repeated 3 times.
TABLE 2 results of acid soluble protein to crude protein ratio in fermented sesame seed meal
Analysis from table 2 shows that: under the same conditions, the addition amount of alkaline protease is increased, the ratio of acid soluble protein to crude protein is slowly increased, and when the addition amount of alkaline protease is 2%, the enzymolysis effect is better, and after that, the addition amount of alkaline protease is increased, the difference of the enzymolysis effect is not obvious. The difference of enzymolysis effect of 1-2% of addition of different types of alkaline proteases is not obvious. In order to save cost, in the industrial production process, 1% of additive amount can be used for enzymolysis.
Test of the influence of the three pH values on the acid-soluble protein content in the fermented sesame seed meal
Referring to steps 1-3 of example 1, only one enzymatic hydrolysis reaction was performed. And (3) mixing alkaline protease A, B and C with sesame seed meal after 40-mesh sieving according to the addition amount of 1.0% and with no alkaline protease as a reference, wherein the ratio of material to water is 3:2, the pH values are respectively adjusted to 9.5, 10.5 and 11.5, and carrying out enzymolysis for 4 hours at 55 ℃. The alkaline protease A is a Shandong large bioengineering Co-Ltd product, the alkaline protease B is a Beijing challenge biotechnology Co-Ltd product, the alkaline protease C is a Xingtai Situte biotechnology Co-Ltd product, the enzyme activity units are 200000U/g, and each group of tests is repeated 3 times.
TABLE 3 results of acid soluble protein to crude protein ratio in fermented sesame seed meal
Analysis from table 3 shows that: under the same conditions, the pH value is increased, the acid-soluble protein is slowly increased to account for the crude protein, the enzymolysis effect is optimal when the enzymolysis pH value is 11.5, and then the pH value is increased, so that the enzymolysis effect is inferior to the ratio of the acid-soluble protein to the crude protein when the pH value is 11.5. The difference of enzymolysis effects of different types of alkaline proteases with pH of 10.5-11.5 is not obvious. In the industrial production process, the pH of 10.5 can be used for enzymolysis for saving cost. Test of influence of the cooperation of four enzymes on acid soluble protein, lactic acid and phytic acid content in fermented sesame seed meal
The test mainly researches the influence of the cooperation of the bacterial enzymes on the content of acid soluble protein, lactic acid and phytic acid in the fermented sesame seed meal, wherein the influence of the cooperation of the bacterial enzymes on the content of the acid soluble protein, the lactic acid and the phytic acid in the fermented sesame seed meal is obtained by comparing the types of alkaline protease in the first-step enzymolysis reaction, the joint use of the types of phytase and lactobacillus plantarum in the second-step enzymolysis fermentation reaction and the reaction comparison of any one of the steps. The test protocol is shown in Table 4. Phytase A, B and C are commercially available single enzymes, the activity units are 10000U/g, A is Wuhan Xinhua Yangshen biological Co., ltd, B is Beijing challenge biotechnology Co., ltd, and C is Guangdong Yiduoli biotechnology Co., ltd.
Table 4 test protocol
TABLE 5 detection results of acid soluble protein, lactic acid and phytic acid degradation rate in fermented sesame seed meal
The test results in Table 5 show that: the acid soluble protein obtained by adopting the two-step reaction (participating in treatment groups 1-9) accounts for more than or equal to 50% of crude protein, the lactic acid content is more than or equal to 42g/kg, and the phytic acid degradation rate is more than or equal to 65%; the treatment groups 1-6 show that when the enzymolysis effects of different phytases are compared, the difference is not obvious, but the addition amount change of the phytases can obviously influence the synergism of the bacteria enzymes on the acid soluble protein, the lactic acid and the phytic acid content in the fermented sesame meal, wherein the addition amount effect of 0.03% of the phytases is better. The content of acid soluble protein, lactic acid and phytic acid in the fermented sesame seed meal can be influenced by the change of the adding amount of the lactobacillus plantarum BFC1602, wherein the enzymolysis effect is better along with the increase of the adding amount, and the difference is not obvious when the adding amount of the lactobacillus plantarum BFC1602 is 0.1 percent and 0.2 percent respectively. Thus, during the production process, the phytase is used in an amount of 0.03% (w/w) and the Lactobacillus plantarum BFC1602 is used in combination in an amount of 0.1% (w/w). Under the same condition, the enzymolysis temperature of the second step reaction is increased, the content of acid soluble protein, lactic acid and phytic acid in the fermented sesame seed meal is slowly increased, the enzymolysis effect is better when the enzymolysis temperature is 35 ℃, and the enzymolysis effect difference is not obvious when the temperature is increased by 45 ℃. Under the same condition, the second reaction time is prolonged, the acid soluble protein, lactic acid and phytic acid content in the fermented sesame seed meal is slowly increased, and when the enzymolysis time is 3d, the enzymolysis effect is better, and after that, the fermentation time is prolonged for 5d, the enzymolysis effect difference is not obvious. The comparison group shows that the effect of the single use of any one step of enzymolysis or fermentation of sesame seed meal is not good in combination, and the bacterial enzyme combination has a synergistic technical effect when applied to the sesame seed meal fermentation method. Namely, alkaline protease is used for carrying out the first-step enzymolysis of sesame seed meal, and phytase and lactobacillus plantarum BFC1602 are combined with the second-step enzymolysis to ferment the sesame seed meal, so that the effects of acid soluble protein content, lactic acid content and phytic acid degradation rate are optimal. The two enzymes are exchanged through the control group 5, the phytase is used for enzymolysis in the first step, the alkaline protease and the lactobacillus plantarum are used for fermentation in the second step, the effect is obviously lower than that of the first step of enzymolysis by the alkaline protease, and then the phytase and the lactobacillus plantarum are used for combined fermentation of sesame seed meal, so that the two-step method is described, namely the first step of enzymolysis reaction by the alkaline protease, then the phytase and the lactobacillus plantarum are used for fermentation in the second step of fermentation, and the effect of the sesame seed meal is the best in the production of low-phytic acid high-acid protein-soluble high-organic acid fermentation. By using another Lactobacillus plantarum BFC190204 strain with a viable count of 2.0X10 10 U/g as a control group 6, we found that the effect of the combined fermentation with phytase was not as good as that of Lactobacillus plantarum BFC1602 strain of the application.
Test of influence of five-fermentation enzymolysis of sesame seed meal to laying hen feed on laying hen production performance
1 Ten thousands of Beijing powder No.1 laying hens with normal feeding, similar weight, consistent laying rate and healthy laying period of 224 days are selected and randomly divided into two groups, namely a control group and a test group, each group is divided into 5 treatment groups, and 1000 treatment groups are respectively selected. Wherein the control group is fed with basic ration (see table 6), the test group is fed with ration with 5% of nitrogen replaced by soybean meal (the preparation method of the fermented and hydrolyzed sesame meal is the same as that of example 1) prepared by the process, the positive test period is 8 weeks, and the test is carried out on a laying hen breeding demonstration base of the national engineering research center for biological feed development. And (3) feeding management: the test chickens eat and drink water freely, and the feeding management is carried out according to the current scheme of the demonstration base. And counting the egg laying quantity, the feed consumption, the average egg weight, the feed-egg ratio, the egg laying rate and the like of each group of chickens in the test period. Measuring the index: the production performance indexes are laying rate, average egg weight, feed-egg ratio, death rate, feed consumption and the like. And (3) data processing: results were statistically analyzed using SAS statistical software ANOVA procedure and multiple comparisons were made using Duncan's method. Results are expressed as mean ± standard deviation. P <0.05 was used as a criterion for judging significance.
TABLE 6 basal ration
TABLE 7 influence of fermented and enzymatically hydrolyzed sesame seed meal on the production performance of laying hens
Note that: the same column of data shoulder letters differ by a significant difference (P < 0.05), and the same letters or no letters indicate that the difference is not significant (P > 0.05).
The test results in Table 7 show that: the egg ratio of the fermented and enzymolyzed sesame seed meal and the daily average consumption have remarkable influence (P is less than 0.05), wherein in a test group, the egg ratio is reduced by 8.23%, and the daily average consumption is reduced by 7.86%; the effect on the laying rate, average egg weight and death rate was insignificant (P > 0.05).
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The preparation method of the fermented sesame seed meal with low phytic acid, high acid protein and high organic acid is characterized by comprising the following steps of:
Step 1, crushing sesame seed meal, sieving, adding alkaline protease according to the addition amount of 0.5% -2.0% (w/w), and uniformly mixing with the sieved sesame seed meal;
Step 2, uniformly mixing the mixed material in the step 1 with water according to the weight ratio of (1-3), and regulating the pH value to be 9.5-11.5;
Step 3, controlling the temperature of the materials to be 45-60 ℃ and carrying out enzymolysis for 2-8 hours, wherein the enzymolysis process is continuously stirred;
step 4, adjusting the pH value of the material subjected to enzymolysis in the step 3 to 5.5-7.0;
Step 5, the phytase is dissolved in water solution according to 0.01 to 0.03 percent (w/w) and lactobacillus plantarum (Lactobacillus Plantarum) BFC1602 according to 0.1 percent (w/w), and the mixture is added into the material obtained in the step 4 to be uniformly mixed, the water content is controlled to be 50 to 80 percent, and the temperature is controlled to be 35 to 45 ℃ for anaerobic fermentation for 3 days; the preservation number of the lactobacillus plantarum BFC1602 is CGMCC No. 13132;
Drying the fermented material in the step6 at high temperature until the water content is less than or equal to 12%; pulverizing, and sieving to obtain the fermented enzymolysis sesame seed meal product with low phytic acid, high acid soluble protein and high organic acid.
2. The method of claim 1, wherein the alkaline protease activity unit of step 1 is 200000U/g added in an amount of 1.0% -2.0% (w/w); the phytase activity unit of the step 5 is 10000U/g, the lactobacillus plantarum (Lactobacillus Plantarum) BFC1602 has a viable count of more than or equal to 2.0X10 10 CFU/g.
3. The method according to claim 1, wherein the feed water ratio of step 2 is 3:2 and the pH value is 10.5-11.5.
4. The method according to claim 1, wherein the material temperature during enzymolysis in the step 3 is 55-60 ℃ and the enzymolysis time is 4h.
5. The method of claim 1, wherein the moisture of step 5 is 50%, and the fermentation temperature is 35 ℃ for 3d.
6. A fermented sesame seed meal with low phytic acid, high acid soluble protein and high organic acid produced by the production method of claim 1.
7. A feed for layer chicken prepared from the fermented sesame seed meal with low phytic acid, high acid protein and high organic acid of claim 6, wherein nitrogen such as fermented enzymolysis sesame seed meal is used for replacing 5% of daily ration of soybean meal.
8. Use of the fermented sesame seed meal of claim 6 and the layer feed of claim 7 for reducing feed-to-egg ratio and daily average consumption.
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