CN115927072A

CN115927072A - Aflatoxin B capable of efficiently degrading 1 Bacillus megaterium mixed with zearalenone, application thereof and bacterium preparation

Info

Publication number: CN115927072A
Application number: CN202211168173.5A
Authority: CN
Inventors: 谢岩黎; 程思忠; 李倩; 孙淑敏; 杨玉辉; 马卫宾; 刘晨; 曹荣耀
Original assignee: Henan University of Technology
Current assignee: Henan University of Technology
Priority date: 2022-09-23
Filing date: 2022-09-23
Publication date: 2023-04-07
Anticipated expiration: 2042-09-23
Also published as: CN115927072B

Abstract

The invention relates to a strain for efficiently degrading aflatoxin B ₁ And zearalenone, and application and bacterial preparation thereof, belonging to the technical field of microbial application. The invention separates and screens the bacillus megatherium HNGD-A6 with the preservation number of CGMCC NO.25222. The bacillus megaterium HNGD-A6 can efficiently degrade aflatoxin B ₁ And zearalenone, and further research shows that aflatoxin B is degraded ₁ And the effective component of the strain of zearalenone is extracellular substances secreted by fermentation of bacillus megaterium HNGD-A6. Simultaneously, the microbial preparation prepared from the bacillus megatherium HNGD-A6 has the effect of treating aflatoxin B ₁ And zearalenone has strong degradation capability and stable degradation state, has huge production and application potentials, and provides a new microbial resource for detoxicating mycotoxin.

Description

Aflatoxin B capable of efficiently degrading 1 Bacillus megaterium mixed with zearalenone, application thereof and bacterium preparation

Technical Field

The invention relates to a strain for efficiently degrading aflatoxin B ₁ And zearalenone, and application and a bacterial preparation thereof, belonging to the technical field of microbial application.

Background

Aflatoxin B ₁ (Aflatoxin B1，AFB ₁ ) Is a secondary metabolite containing a bifuran ring and an o-naphthone structure and produced by aspergillus flavus, aspergillus terreus, aspergillus parasiticus and the like. In 1993, AFB was released by International agency for research on cancer (IARC) ₁ Listed as category 1 chemical carcinogen, which is a natural compound identified so far with the strongest toxicity and the strongest ability of causing liver cancer. Has strong carcinogenicity, teratogenicity, mutagenicity, immunity inhibiting and liver injury toxic effects on human and animals. AFB ₁ The loss of the food industry in China is huge every year, and the food safety in China is also threatened strongly, so how to solve the AFB from the root ₁ The problem of contamination is of particular importance. Due to AFB ₁ Various methods of elimination or reduction have been proposed, including physical, chemical and biological methods. The traditional physical method and chemical method can damage the food, influence the color, the fragrance, the taste and the nutritive value of the food, and even cause certain harm to human bodies, and biological detoxification belongs to a safe, efficient, feasible and environment-friendly method.

Zearalenone (ZEN) and derivatives thereof are a class of non-steroidal estrogenic mycotoxins mainly produced by fusarium fungi and are widely present in cereal feeds such as corn, barley, wheat and sorghum and byproducts thereof. ZEN enters a food chain through polluted grain agricultural and sideline products and feed, and after being absorbed by animals, the ZEN causes the estrogen comprehensive symptoms of the livestock, causes the phenomena of infertility, abortion and miscarriage in the bodies of the livestock, has strong carcinogenicity, and seriously harms the health of the livestock and human beings. The main methods for detoxifying ZEN at present are a physical method, a chemical method and a biological method. The traditional physical and chemical method can not effectively remove the toxin in the grains, can damage the nutrient components of the grains, influences the taste of the food, and even causes secondary pollution, so that detoxification of the ZEN and the derivatives thereof by utilizing the biological engineering technology is a main method for solving the problem in the future.

Disclosure of Invention

In order to solve the problems, the first purpose of the invention is to provide a strain for efficiently degrading aflatoxin B ₁ And zearalenone (Bacillus megaterium) HNGD-A6.

The bacillus megatherium HNGD-A6 can efficiently degrade aflatoxin B ₁ And zearalenone, the fermentation broth of which is directed against aflatoxin B ₁ The degradation rate of the compound reaches 93.5 percent, and the degradation rate of the compound to the zearalenone reaches 97.1 percent.

A second object of the present invention is to provide the use of Bacillus megaterium HNGD-A6 for the degradation of mycotoxins.

The third purpose of the invention is to provide the application of Bacillus megaterium HNGD-A6 in preparing the microbial preparation for degrading mycotoxin.

The fourth purpose of the invention is to provide a microbial preparation with the function of degrading mycotoxin.

The fifth purpose of the invention is to provide the application of the microbial preparation in the degradation of mycotoxin.

In order to achieve the purpose, the invention adopts the technical scheme that:

aflatoxin B capable of efficiently degrading ₁ And zearalenone, wherein the strain is Bacillus megaterium (Bacillus megaterium) HNGD-A6, and the preservation unit is as follows: china general microbiological culture Collection center (CGMCC) with a collection number of CGMCC NO.25222.

The invention separates and screens bacillus megatherium HNGD-A6 from the soil of corn field near river-south university in Guangzhou city, which can efficiently degrade aflatoxin B ₁ And zearalenone, can be widely used for detoxifying mycotoxins in grains, feeds and other processing byproducts thereof.

Application of Bacillus megaterium (Bacillus megaterium) HNGD-A6 in degrading mycotoxin is provided.

Bacillus megaterium (Bacillus megaterium) HNGD-A6 can efficiently degrade aflatoxin B at the same time ₁ And zearalenone, which can be applied to the degradation of mycotoxins.

Preferably, the mycotoxin is aflatoxin B ₁ And zearalenone.

Further preferably, the mycotoxin is degraded by a Bacillus megaterium HNGD-A6 fermentation broth or a fermentation supernatant or a bacterial suspension or an intracellular extract.

Proved by HNGD-A6 fermentation liquor, fermentation supernatant, thallus suspension and intracellular extract to aflatoxin B ₁ And zearalenone. In degrading aflatoxin B ₁ In the process, the degradation rate of HNGD-A6 fermentation liquor is 93.5%, the degradation rate of fermentation supernatant is 88.2%, the degradation rate of thallus suspension is 43.25%, and the degradation rate of intracellular extract is 20.3%; when the zearalenone is degraded, the degradation rate of the HNGD-A6 fermentation liquor is 97.1%, the degradation rate of the fermentation supernatant is 61.2%, the degradation rate of the thallus suspension is 33.8%, and the degradation rate of the intracellular extract is 11.8%. The HNGD-A6 fermentation liquor and the fermentation supernatant have good degradation effect on the mycotoxin, so the HNGD-A6 fermentation liquor or the fermentation supernatant is preferentially selected in practical application.

Application of Bacillus megaterium (Bacillus megaterium) HNGD-A6 in preparation of mycotoxin degrading microbial preparation.

Bacillus megaterium (Bacillus megaterium) HNGD-A6 can be prepared into a microbial preparation for degrading mycotoxin.

Preferably, the mycotoxin is aflatoxin B ₁ And zearalenone.

A microbial preparation with effect of degrading mycotoxin comprises Bacillus megaterium (HNGD-A6) fermentation liquid or fermentation supernatant.

The microbial preparation comprises HNGD-A6 fermentation liquor or fermentation supernatant, and also can comprise active substances (such as extracellular enzyme) such as metabolites extracted from the fermentation liquor or the fermentation supernatant.

The application of the microbial preparation in degradation of mycotoxin.

The microbial agent can efficiently degrade aflatoxin B ₁ And zearalenone, which can be widely used as an additive in grain feeds such as corn, barley, wheat and sorghum and byproducts thereof for mycotoxin detoxification.

Preferably, the mycotoxin is aflatoxin B ₁ And zearalenone.

Drawings

FIG. 1 shows that the bacterial strain in example 1 of the invention degrades aflatoxin B ₁ High performance liquid chromatograms of the pre-and post-degradation;

FIG. 2 is a morphological diagram of a colony in example 2 of the present invention;

FIG. 3 is a phylogenetic tree constructed based on the 16S rDNA gene sequence in example 2 of the present invention;

FIG. 4 is a high performance liquid chromatogram of the strain of example 3 before and after degradation of zearalenone;

FIG. 5 shows that the different components of the strain in example 4 of the present invention degrade aflatoxin B ₁ A graph comparing zearalenone;

FIG. 6 shows that the strain degrades aflatoxin B simultaneously in example 5 of the present invention ₁ And zearalenoneA liquid chromatogram map;

FIG. 7 shows that the microbial preparation degrades aflatoxin B in example 6 of the invention ₁ And high performance liquid chromatography of zearalenone.

Detailed Description

The present invention will be further described with reference to specific embodiments, but the scope of the present invention is not limited thereto; unless otherwise specified, reagents, instruments and the like used in examples are commercially available.

The following examples and test examples are briefly described in some of the biological materials, reagents, and devices involved in the following examples:

culture medium:

horrmisch modified screening medium (1L): 0.25g KH ₂ PO ₄ ，1.0g NH ₄ NO ₃ ，1.0g CaCl ₂ ，0.25g MgSO ₄ .7H2O，1.0mg FeSO ₄ 20g agar, 1g coumarin, pH 7.0.

Seed medium (1L): 3g of beef extract; 10g of peptone; 5g NaCl, pH 7.0.

LB medium (1L): 10g of tryptone, 5g of yeast extract, 10g of NaCl, pH 7.0.

1. Aflatoxin B capable of efficiently degrading ₁ And zearalenone

Aflatoxin B capable of efficiently degrading ₁ And zearalenone, wherein the strain is Bacillus megaterium (Bacillus megaterium) HNGD-A6, and the preservation number is CGMCC NO:25222; the preservation date is as follows: 7 months and 1 day 2022; the preservation unit: china general microbiological culture Collection center, the preservation Address: beijing, chaoyang district, beichen Xilu No. 1 institute, institute of microbiology, china academy of sciences.

Example 1 isolation and screening of Bacillus megaterium HNGD-A6

1. Bacterial strain preliminary screening

Removing impurities from soil sample collected from corn field near river university of Guangzhou river, weighing 10g of the sample, placing in 90mL of sterile physiological saline, shaking at constant temperature of 150r/min for 2h, sucking 0.20mL of the sample, and mixingActivating the homogenized solution in sterilized 5mL seed culture solution, setting the culture conditions of air bath shaking table at 150r/min, 37 deg.C and 24h, diluting the activated solution with equal gradient, and diluting to 10% ^-1 、10 ^-2 、10 ^-3 、10 ^-4 、10 ^-5 、10 ^-6 、10 ^-7 、10 ^-8 And then sucking 0.2mL of each gradient diluent, coating the gradient diluent on a Horrmisch improved screening culture medium (taking coumarin as a unique carbon source) plate, placing the gradient diluent in an incubator at 37 ℃, carrying out inverted culture for 3-7 d, and observing the growth condition of the strain. Selecting strains with good growth, performing streak passage on a primary screening culture medium plate, and storing single strains obtained after 3 passages in a refrigerator at-80 deg.C with 40% glycerol.

2. Degradation of aflatoxin B ₁ Function verification of

The purified primary screening bacterial strain is selected and inoculated in 50mL of seed culture medium for activated fermentation, the bacterial strain is inoculated in 50mL of LB liquid culture medium for fermentation with the inoculum size of 0.01 percent, and the culture condition of a gas bath shaker is set as 150r/min, 37 ℃ and 48h. 975. Mu.L of strain fermentation broth and 25. Mu.L of AFB were taken ₁ Standard (100. Mu.g/mL) was placed in a 10mL sterile centrifuge tube and vortexed for 30s to induce AFB ₁ The concentration is 2.5 mug/mL, and AFB is added into a sterile fermentation medium ₁ As a blank control. The conditions of the gas bath shaking table culture were set at 150r/min, 37 ℃ and 72h, and 3 parallel tests were set for each group. After the reaction, 3 volumes of dichloromethane were added to the centrifuge tube and vortex-extracted for 60s each time, the aqueous layer was removed and discarded, the dichloromethane layer was placed in a nitrogen blower, slowly dried under nitrogen at 35 ℃, the residue was dissolved with 0.5mL of mobile phase (water: methanol: acetonitrile =6: 2), vortex-extracted for 60s, and filtered through a 0.22 μm organic phase filter membrane.

Detection of residual Aflatoxin B Using HPLC ₁ The content of (a). HPLC detection conditions are as follows, and the chromatographic column comprises: c18 column (150 mm. Times.4.6 mm,4 μm); mobile phase: water: methanol: acetonitrile (60; the detection wavelength is 365nm; flow rate: 1.0mL/min; sample introduction amount: 20 μ L.

In the formula: a. The ₀ Control group Aflatoxin B ₁ The content; a. The ₁ Aflatoxin B for the experimental group ₁ And (4) content.

The results are shown in FIG. 1, which illustrates the aflatoxin B pair of the bacterium ₁ The degradation rate of (2) was 93.5%.

Example 2 identification of Bacillus megaterium HNGD-A6

1. Morphological identification

Bacterial colonies of the strain grow abundantly on a solid LB culture medium, are round and light yellow, have neat edges and smooth surfaces, and are shown in figure 2.

2. Molecular biological identification

The bacterial DNA was extracted using an Ezup column type bacterial genomic DNA extraction kit using primers 27F (5 '-AGTTTGATCTMTGGCTCAG-3') and 1492R (5-. 16S rDNA was amplified using the genomic DNA as a template. The reaction system is; 10 XBuffer (with Mg2 +) 2.5. Mu.L, F (10 uM) 0.5. Mu.L, R (10 uM) 0.5. Mu.L, dd H2O 25. Mu.L, template (genomic DNA20-50 ng/. Mu.L) 0.5. Mu.L, dNTPs (each 2.5 mM) 1. Mu.L, enzyme 0.2. Mu.L. The amplification procedure is as follows; (1) pre-denaturation at 94 ℃ for 4min; (2) denaturation at 94 ℃ for 45s; (3) annealing at 55 ℃ for 45s; (4) extension at 72 ℃ for 1min; (6) the steps (2) to (4) are circulated for 30 times; (6) repairing and extending for 10min at 72 ℃. Carrying out electrophoresis on 1% agarose, carrying out electrophoresis observation at 150V and 100mA for 20min, observing a strip under an ultraviolet lamp after the electrophoresis is finished, and judging whether the PCR amplification is successful. The amplified products were purified and sequenced by Shanghai Bionics GmbH, and the sequencing results were compared at NCBI database.

The 16S rDNA gene sequence of the strain HNGD-A6 obtained by sequencing has 1458 basic groups, homology analysis is carried out, a phylogenetic tree is constructed by MEGA 6.0 software, the phylogenetic tree is shown in figure 3, and the similarity between the strain HNGD-A6 and the bacillus megatherium is highest.

3. Physiological and biochemical characterization

The physiological and biochemical characteristics are shown in Table 1 (+ positive, -negative).

By combining morphological characteristics, physiological and biochemical characteristics and results of 16S rDNA sequencing and homology analysis, the strain is identified as Bacillus megaterium (named as Bacillus megaterium HNGD-A6), which has been deposited in China general microbiological culture Collection center on 7-01.2022, with the addresses of: west road No. 1, north chen, chaoyang, beijing, no. 3, zip code 100101; the preservation number is CGMCC NO.25222.

2. Application of bacillus megaterium in degrading mycotoxin

The invention detects the aflatoxin B of the bacillus megatherium HNGD-A6 by HPLC ₁ And the degradation effect of zearalenone, and also degrades aflatoxin B on bacillus megatherium HNGD-A6 ₁ And the characteristics of zearalenone are studied, and the result proves that HNGD-A6 can efficiently degrade aflatoxin B at the same time ₁ And zearalenone, HNGD-A6 fermentation liquor, fermentation supernatant, thallus suspension and intracellular extract on aflatoxin B ₁ And zearalenone, so that the degradation effect of the fermentation liquor is optimal.

Example 3 analysis of the ability of Bacillus megaterium HNGD-A6 to degrade zearalenone

Placing 950 μ L of Bacillus megaterium HNGD-A6 fermentation liquid and 50 μ L of zearalenone standard (100 μ g/mL) in 10mL sterilized centrifuge tube, vortex for 30s to make the concentration of zearalenone 5 μ g/mL, and adding zearalenone into sterile fermentation medium as blank control. The conditions of the gas bath shaking table culture were set at 150r/min, 37 ℃ and 72h, and 3 parallel tests were set for each group. After the reaction, 3 volumes of dichloromethane were added to the centrifuge tube and vortex-extracted for 60s each time, the aqueous layer was removed and discarded, the dichloromethane layer was placed in a nitrogen blower, slowly dried under nitrogen at 35 ℃, the residue was dissolved using 1mL of mobile phase (water: methanol = 2) and filtered through a 0.22 μm organic phase filter membrane by vortex-shaking 60s.

The detection conditions for detecting zearalenone by using HPLC are as follows: a chromatographic column: c18 column (250 mm. Times.4.6 mm,4 μm); mobile phase: methanol-water (80; the detection wavelength is 365nm; column temperature: 30 ℃; flow rate: 1.0mL/min; sample introduction amount: 20 μ L.

In the formula: b ₀ The content of zearalenone in the control group; b is ₁ The test group showed zearalenone content.

The result is shown in figure 4, and the result shows that the degradation rate of HNGD-A6 to zearalenone is as high as 97.1%.

Example 4 degradation of Aflatoxin B by HNGD-A6 ₁ And zearalenone characteristic analysis

1. Degradation experiment of fermentation supernatant

Centrifuging the strain fermentation liquor fermented for 48h at 4 ℃ and 10000r/min for 10min to obtain fermentation supernatant.

Collecting 975 μ L fermentation supernatant and 25 μ L AFB ₁ Standards (100. Mu.g/mL) were mixed well to a final concentration of 2.5. Mu.g/mL. Detection of aflatoxin B by HPLC was performed according to the conditions and procedures in example 1 ₁ The degradation ability of (a); mu.L of the fermentation supernatant was mixed with 50. Mu.L of zearalenone standard (100. Mu.g/mL) to a final concentration of 2.5. Mu.g/mL. The degradation ability of zearalenone was examined by high performance liquid chromatography under the conditions and procedures as in example 3.

2. Degradation experiment of thallus suspension

After the strain fermentation liquor is centrifuged, supernatant is removed, the residual thallus precipitate is washed twice by Phosphate Buffered Saline (PBS), and 5 times of PBS is added to form thallus suspension.

975. Mu.L of the cell suspension and 25. Mu.L of AFB were taken ₁ Standards (100. Mu.g/mL) were mixed well to a final concentration of 2.5. Mu.g/mL. Detection of Aflatoxin B by high performance liquid chromatography using the conditions and procedures of example 1 ₁ The degradation ability of (a); mu.L of the cell suspension was mixed with 50. Mu.L of zearalenone standard (100. Mu.g/mL) to a final concentration of 2.5. Mu.g/mL. The degradation ability of zearalenone was examined by high performance liquid chromatography under the conditions and procedures as in example 3.

3. Degradation test of intracellular extracts

Crushing the thallus suspension by using an ultrasonic cell crusher, centrifuging the crushed thallus suspension at 10000r/min and 4 ℃ for 10min, and taking supernatant to pass through a 0.22 mu m filter membrane to obtain an intracellular extract.

Mixing 975 μ L intracellular extract with 25 μ L AFB ₁ Standards (100. Mu.g/mL) were mixed well to a final concentration of 2.5. Mu.g/mL. Detection of aflatoxin B by HPLC was performed according to the conditions and procedures in example 1 ₁ The degradation ability of (c); mu.L of the intracellular extract was mixed with 50. Mu.L of zearalenone standard (100. Mu.g/mL) to a final concentration of 2.5. Mu.g/mL. The degradation ability of zearalenone was examined by high performance liquid chromatography under the conditions and procedures as in example 3.

HNGD-A6 to aflatoxin B ₁ The degradation effect of (2) was as shown in FIG. 5, the degradation rate of the fermentation supernatant was 88.2%, the degradation rate of the cell suspension was 43.25%, and the degradation rate of the intracellular extract was 20.3%. Wherein the degradation effect of the fermentation supernatant is optimal.

The degradation effect of HNGD-A6 on zearalenone is shown in FIG. 5, where the degradation rate of fermentation supernatant is 61.2%, the degradation rate of cell suspension is 33.8%, and the degradation rate of intracellular extract is 11.8%. Wherein the degradation effect of the fermentation supernatant is optimal.

The best degradation of the fermentation supernatant is probably due to the fact that some extracellular substances produced by the fermentation of the strain, such as extracellular enzymes, play a major role.

Example 5 HNGD-A6 Simultaneous degradation of Aflatoxin B ₁ Analysis of the ability to zearalenone

Placing 950 μ L of HNGD-A6 strain fermentation liquid in 10mL sterilized centrifuge tube, adding 25 μ L of aflatoxin B ₁ The test for degradation rates of aflatoxin B1 and zearalenone were carried out by using a HPLC (high performance liquid chromatography) under the conditions and procedures of example 1 and example 3, respectively, using a standard (100. Mu.g/mL) and 25. Mu.L of a zearalenone standard (100. Mu.g/mL) and replacing the bacterial solution with a sterile fermentation medium for the control group.

The results are shown in FIG. 6, where HNGD-A6 is directed to aflatoxin B ₁ The degradation rate is 92.6 percent, and HNGD-A6 degrades zearalenoneThe ratio was 97.4%. The result proves that the degradation effect of HNGD-A6 is still efficient and stable under the condition that two toxins exist simultaneously.

3. Microbial preparation with mycotoxin degrading effect

Aflatoxin B of HNGD-A6 fermentation liquor, fermentation supernatant, thallus suspension and intracellular extract ₁ And zearalenone, wherein the HNGD-A6 fermentation liquid and the fermentation supernatant have good degradation effect, and can be treated by means commonly used in the field to obtain the microbial preparation.

Example 6A microbial preparation having mycotoxin degrading action

The microbial preparation with the mycotoxin degrading effect of the embodiment is prepared in the following way: 20mL of HNGD-A6 fermentation liquor is pre-frozen in a sample plate at the temperature of minus 80 ℃ for 1h, and vacuum freeze-dried at the temperature of minus 60 ℃ for 12h to obtain the microbial preparation.

4. Application of microbial preparation in degradation of mycotoxin

Example 7 microbial preparation degradation of Aflatoxin B ₁ And zearalenone ability analysis

Taking the microbial preparation prepared in the example 6, re-dissolving and concentrating the microbial preparation by using 5mL of PBS, putting 950 mu L of HNGD-A6 microbial preparation into a 10mL sterile centrifuge tube, and adding 25 mu L of aflatoxin B ₁ The standard (100. Mu.g/mL) and 25. Mu.L zearalenone standard (100. Mu.g/mL) were mixed, and the control group was treated with a sterile fermentation medium instead of the bacterial solution by HPLC under the conditions and procedures of example 1 and example 3 to obtain aflatoxin B ₁ And detecting the degradation rate of the zearalenone.

The results are shown in FIG. 7, and aflatoxin B ₁ The degradation rate reaches 91.8 percent, the degradation rate of the zearalenone reaches 75.1 percent, which indicates that the microbial preparation prepared from the HNGD-A6 fermentation liquor can be used for treating aflatoxin B ₁ And zearalenone still have a high degradation capacity.

The above embodiments are merely preferred embodiments of the present invention, and not intended to limit the scope of the invention, so that equivalent changes or modifications made based on the structure, characteristics and principles of the invention should be included in the scope of the invention.

Claims

1. Aflatoxin B capable of efficiently degrading ₁ And zearalenone (Bacillus megaterium), characterized in that: the strain is Bacillus megaterium (Bacillus megaterium) HNGD-A6, and the preservation unit is as follows: china general microbiological culture Collection center (CGMCC) with a collection number of CGMCC NO.25222.

2. Use of a Bacillus megaterium according to claim 1 for the degradation of mycotoxins.

3. Use of Bacillus megaterium (Bacillus megaterium) according to claim 2 for the degradation of mycotoxins, characterized in that: the mycotoxin is aflatoxin B ₁ And zearalenone.

4. Use of Bacillus megaterium (Bacillus megaterium) according to claim 3 for the degradation of mycotoxins, characterized in that: the mycotoxin is degraded by Bacillus megaterium (Bacillus megaterium) HNGD-A6 fermentation liquor or fermentation supernatant or thallus suspension or intracellular extract.

5. The method for efficiently degrading aflatoxin B as claimed in claim 1 ₁ And zearalenone in the preparation of a mycotoxin degrading microbial preparation.

6. Use of a Bacillus megaterium (Bacillus megaterium) according to claim 5 for the preparation of a microbial preparation for the degradation of mycotoxins, characterized in that: the mycotoxin is aflatoxin B ₁ And zearalenone.

7. Aflatoxin B with degradation function ₁ The microbial preparation acted with the zearalenone is characterized in that: comprising a Bacillus megaterium (Bacillus megaterium) HNGD-A6 fermentation broth or supernatant according to claim 1.

8. Use of a microbial preparation according to claim 7 for the degradation of mycotoxins.

9. Use of a microbial preparation according to claim 8 for the degradation of mycotoxins, characterized in that: the mycotoxin is aflatoxin B ₁ And zearalenone.