CN115872583A - Method for enhancing anaerobic methane production based on biochar-anaerobic granular sludge - Google Patents
Method for enhancing anaerobic methane production based on biochar-anaerobic granular sludge Download PDFInfo
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- CN115872583A CN115872583A CN202211594761.5A CN202211594761A CN115872583A CN 115872583 A CN115872583 A CN 115872583A CN 202211594761 A CN202211594761 A CN 202211594761A CN 115872583 A CN115872583 A CN 115872583A
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Abstract
The invention discloses a method for enhancing anaerobic methanogenesis based on biochar-anaerobic granular sludge, which comprises the steps of adding biochar and operating an up-flow anaerobic sludge bed to culture anaerobic granular sludge, wherein the anaerobic granular sludge forms a stable biochar-anaerobic granular sludge polymer by adsorbing and wrapping the biochar through extracellular polymers. On one hand, the loss and the loss of the biochar can be reduced; on the other hand, the tight combination of the biochar and anaerobic sludge particles can obviously improve the reaction rate, promote the acid production and the methane production, and improve the income brought by the methane production through the anaerobic digestion of the sludge. The method has the advantages that the corn straw biochar is wide in source, green, environment-friendly, cheap and easy to obtain, so that the technology has the potential of wide application; the methane yield is obviously improved when the organic wastewater is treated, and the method can be widely applied to sewage plants.
Description
Technical Field
The invention relates to the technical field of organic wastewater treatment, in particular to a method for enhancing anaerobic methanogenesis based on biochar-anaerobic granular sludge.
Background
The anaerobic biological treatment technology has low operation cost, low energy consumption and less generated excess sludge, is suitable for treating organic wastewater which is difficult to be biologically degraded under high-concentration and aerobic conditions, can recover partial biomass energy, and is a green low-carbon sewage treatment means. The successful culture of the anaerobic granular sludge greatly improves the anaerobic treatment performance, compared with anaerobic activated sludge, the anaerobic activated sludge has good sedimentation performance, long sludge age and high biomass, is easier to remain in a sludge bed tank under the conditions of high gas yield and upward flow velocity, and can bear higher organic load and hydraulic load. However, the problems of low methane conversion rate caused by organic acid accumulation or environmental disturbance still exist, the methane conversion rate in the anaerobic treatment process is further improved, and the method has important practical significance for recycling the biomass energy from pollutants.
The discovery of direct specific electron transfer (DIET) processes offers more possibilities to further increase the methanogenic potential. In the path, electrons can be directly transferred to CO between the organic acid oxidizing bacteria and the methanogen with low electron loss or energy consumption 2 Thereby reducing it to CH 4 . While the DIET mechanism based on conductive materials has a better effect in promoting methanogenesis. The conductor material generally has huge specific surface area, high conductivity and stability, and shortens the electron transfer distance between the intercropping microorganisms. Among the conductor materials, the biochar has wide sources, is green and environment-friendly, is cheap and easy to obtain, and has good application prospects. Large-particle biochar is generally selected as a conductor material to be put into an anaerobic methanogenesis system to improve the system performance, because the large-particle biochar can also be used as a filler to load a large amount of microorganisms to improve the biological diversity and stability of the system. However, the large-particle biochar is low in density, so that the large-particle biochar is easy to discharge along with effluent and is easy to be impacted by water power, so that a large amount of biochar is lost and lost, and the cost is increased; in addition, the insufficient contact between the large-particle biochar and the sludge causes the reaction efficiency to be low, which is an inevitable limitation of the biochar on enhancing the performance of an anaerobic digestion system.
Disclosure of Invention
The invention aims to provide a method for enhancing anaerobic methanogenesis based on biochar-anaerobic granular sludge, wherein the biochar and the granular sludge are tightly combined to remarkably accelerate the reaction rate, improve the electron transfer activity, promote the processes of acid production and methane production and effectively improve the methane generation amount.
In order to achieve the above object, the present application provides a method for enhancing anaerobic methanogenesis based on biochar-anaerobic granular sludge, comprising adding biochar and running an up-flow anaerobic sludge bed to culture anaerobic granular sludge, wherein the anaerobic granular sludge forms stable biochar-anaerobic granular sludge polymer by adsorbing and wrapping the biochar with extracellular polymer. The biochar-anaerobic granular sludge can enhance the performance of an anaerobic digestion system and improve the yield of methane.
Further, the preparation method of the biochar comprises the following steps: placing 4-6 cm of corn straw into a muffle furnace, reacting for 9.5-10.5h under the conditions of no oxygen and the temperature of 450-550 ℃, and raising the temperature by 8-9 ℃/min; after the reaction is finished, cooling to room temperature, and grinding and screening to obtain the biochar with the particle size of 10-30 mu m, more preferably 10-15 mu m.
Further, in an up-flow anaerobic sludge bed, sewage enters a granular sludge zone through a water inlet pipe, wastewater, anaerobic granular sludge and biochar are uniformly mixed, wherein the biochar enters a sludge bed tank body after being dispersed in water through an ultrasonic dispersion zone, the pH and the temperature in the sludge bed tank body are observed in real time through a pH probe and a temperature probe, generated gas enters a gas collection tank through a three-phase separator, gas sampling is carried out through a gas outlet, and liquid sampling is carried out through a sampling port; and discharging the effluent into a sedimentation tank through a water outlet, standing and settling, discharging supernatant from a drain pipe, and re-introducing suspended biochar and suspended sludge in the effluent into a sludge bed tank through a return pipe.
Further, the particle size of the anaerobic granular sludge is 1-2mm.
Further, the effective volume of the sludge bed tank body is 10L, the adding amount of the biochar is 2g/L, and the mass ratio of the anaerobic granular sludge concentration to the added biochar is 5:1.
furthermore, the sewage retention time of the sludge bed tank body is 5 days, and the culture period is 30 days.
Furthermore, the biochar is subjected to ultrasonic treatment for 10min in water at the frequency of 40kHz, so that the biochar is uniformly distributed in the water and is pumped into a sludge bed tank body in the form of dispersion liquid.
Furthermore, the biochar enters the upflow anaerobic sludge blanket and is stirred by the submersible stirrers symmetrically arranged in the sludge blanket tank body, so that the biochar is fully contacted with anaerobic granular sludge.
Further, the biochar (with the particle size of 10-30 μm) is far smaller than the anaerobic granular sludge (with the particle size of 1-2 mm), and is contacted with extracellular polymeric substances of the anaerobic granular sludge through stirring so as to be adsorbed on the surface of the anaerobic granular sludge, and then enters the inside of the sludge through secretory coating to form a stable polymer.
Further, the biochar is combined with flocculent sludge to form compact particles which encapsulate the biochar. The biochar-granular sludge polymer can enhance the electron transfer activity of an anaerobic methanation system, improve the electron transfer efficiency and promote the acid production and methane production.
Compared with the prior art, the technical scheme adopted by the invention has the advantages that: the invention can form stable biochar-anaerobic granular sludge polymer to enhance electron transfer activity, improve electron transfer efficiency, promote acid production and effectively improve methane generation. The needed biochar can be prepared by self, is low in price and easy to obtain, has low loss rate and loss rate of the biochar, further saves the cost, can realize waste recycling, and has double benefits to economy and environment.
Drawings
FIG. 1 is a graph of methane cumulative yield;
FIG. 2 is a view showing the structure of an upflow anaerobic sludge blanket;
FIG. 3 is a cross-sectional view of a biochar-anaerobic granular sludge polymer.
Wherein: 1. a water inlet pipe, 2, a granular sludge area, 3, a submersible mixer, 4, a sampling port, 5, an ultrasonic dispersion area, 6, a return pipe, 7, a water outlet, 8, a three-phase separator, 9, an exhaust port, 10, a temperature probe and a pH probe, 11, a sedimentation tank, 12, a drain pipe, 13, a gas collecting tank, 14, anaerobic granular sludge, 15 and biochar.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the application, i.e., the embodiments described are only a subset of, and not all embodiments of the application.
Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present application without making any creative effort, shall fall within the protection scope of the present application.
Example 1
As shown in fig. 1 to 3, the present example provides a method for enhancing anaerobic methanogenesis based on biochar-anaerobic granular sludge by feeding biochar and running an upflow anaerobic sludge bed to culture anaerobic granular sludge forming stable biochar-anaerobic granular sludge polymer by extracellular polymer adsorption and encapsulation of biochar.
The preparation method of the biochar comprises the following steps: putting 4-6 cm of corn straw into a muffle furnace, continuously introducing nitrogen to keep an oxygen-free environment, controlling the temperature rise amplitude to be 8-9 ℃/min, and maintaining for 9.5-10.5h after the temperature reaches 450-550 ℃. After the cracking process is finished, cooling to room temperature, grinding and screening to obtain biochar with the particle size of 10-30 mu m, preferably 10-15 mu m, and drying and storing for later use;
the operation mode of the up-flow anaerobic sludge bed is as follows: the effective volume of a sludge bed tank is 10L, continuous water inflow is adopted, the hydraulic retention time is 5 days, the concentration of volatile suspended solids is 10g/L, organic wastewater (glucose: starch =1, COD is 2 g/L) enters a granular sludge zone 2 from a water inlet pipe 1, the wastewater, granular sludge and biochar are uniformly mixed by a submersible mixer 3 (10 h stops every 2 h), wherein the biochar passes through a biochar ultrasonic dispersion zone 5 and is subjected to ultrasonic treatment for 10min at the frequency of 40kHz in water, the biochar is added once every 6 days, and the concentration of the biochar in the sludge bed tank is 2g/L; a pH probe and a temperature probe 10 are arranged in the sludge bed tank body and are used for observing pH and temperature in real time, generated gas enters a gas collecting tank 13 through a three-phase separator 8, a gas exhaust port 9 is used for gas sampling, and a sampling port 4 is used for liquid sampling. The effluent is discharged into a sedimentation tank 11 through a water outlet 7, supernatant is discharged from a water discharge pipe 12 after standing and sedimentation, suspended biochar and suspended sludge in the effluent enter a sludge bed tank body again through a return pipe 6, and the reaction period is 30 days.
The performance of the cultured biochar-anaerobic granular sludge is characterized in that: respectively taking granular sludge (the particle size is 1-2 mm) in a sludge bed tank body before and after culture, adding the sludge into six 500mL screw bottles, wherein the concentration of volatile suspended solids in the bottles is 1g/L, the COD is 1g/L, taking the granular sludge before adding the biochar for culture as control groups (A1, A2 and A3), taking the granular sludge after adding the biochar for culture as experimental groups (B1, B2 and B3), and introducing N into all the bottles 2 Blowing off for 30min, removing headspace oxygen, sealing with a gland, and placing in a constant temperature air shaking table at 37 deg.C with shaking table frequency of 150r/min; collecting the produced gas by using an aluminum foil gas collection bag, and measuring the volume of the produced gas by using an injector; the gas composition was measured by gas chromatography, and the cumulative amount of methane produced is shown in FIG. 1. The organic matter removing amount of the experimental group is 1.42 times that of the control group, the organic acid generating amount is improved by 30.42 percent, and the electron transfer activity is improved by 80.96 percent. As can be seen from figure 1, the accumulated methane production of the experimental group added with the biochar is obviously higher than that of the control group not added with the biochar, and the methane production of the experimental group is improved by 82.97% compared with that of the control group, so that the methane production of the upflow anaerobic sludge bed is obviously improved by the method.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (10)
1. The method for enhancing anaerobic methanogenesis based on biochar-anaerobic granular sludge is characterized by comprising the steps of adding biochar and operating an up-flow anaerobic sludge bed to culture anaerobic granular sludge, wherein the anaerobic granular sludge adsorbs and wraps the biochar through extracellular polymeric substances to form biochar-anaerobic granular sludge polymers.
2. The method for enhancing anaerobic methanogenesis based on biochar-anaerobic granular sludge according to claim 1, wherein the biochar is prepared by the following steps: placing 4-6 cm of corn straw into a muffle furnace, reacting for 9.5-10.5h under the conditions of no oxygen and the temperature of 450-550 ℃, wherein the temperature rise range is 8-9 ℃/min; cooling to room temperature after the reaction is finished, and grinding and screening to obtain the biochar with the particle size of 10-30 mu m.
3. The method for enhancing anaerobic methanogenesis based on biochar-anaerobic granular sludge as claimed in claim 1, wherein in an up-flow anaerobic sludge bed, sewage enters a granular sludge zone through a water inlet pipe, wastewater, anaerobic granular sludge and biochar are uniformly mixed, wherein biochar enters a sludge bed tank after being dispersed in water through an ultrasonic dispersion zone, the pH and temperature in the sludge bed tank are observed in real time through a pH probe and a temperature probe, generated gas enters a gas collection tank through a three-phase separator, gas sampling is carried out through a gas outlet, and liquid sampling is carried out through a sampling port; and discharging the effluent into a sedimentation tank through a water outlet, standing and settling, discharging supernatant from a drain pipe, and re-introducing suspended biochar and suspended sludge in the effluent into a sludge bed tank through a return pipe.
4. The biochar-based anaerobic granular sludge enhanced anaerobic methanogenesis method according to claim 1, wherein the anaerobic granular sludge has a particle size of 1-2mm.
5. The method for enhancing anaerobic methanogenesis based on biochar-anaerobic granular sludge according to claim 3, wherein the effective volume of the sludge bed tank is 10L, the adding amount of biochar is 2g/L, and the mass ratio of the anaerobic granular sludge concentration to the added biochar is 5:1.
6. the method for enhancing anaerobic methanogenesis based on biochar-anaerobic granular sludge as claimed in claim 3, wherein the sewage retention time of the sludge blanket tank is 5 days and the culture period is 30 days.
7. The method for enhancing anaerobic methanogenesis based on biochar-anaerobic granular sludge as claimed in claim 3, wherein the biochar is ultrasonically treated in water at a frequency of 40kHz for 10min, so that the biochar is uniformly distributed in the water and is pumped into a sludge bed tank body in the form of dispersion liquid.
8. The method for enhancing anaerobic methanogenesis based on biochar-anaerobic granular sludge as claimed in claim 3, wherein the biochar enters the upflow anaerobic sludge blanket and is stirred by submersible stirrers symmetrically arranged in the sludge blanket tank, so that the biochar is fully contacted with the anaerobic granular sludge.
9. The method for enhancing anaerobic methanogenesis based on biochar-anaerobic granular sludge according to claim 1, wherein the biochar particle size is far smaller than the anaerobic granular sludge particle size, and the biochar is contacted with extracellular polymers of the anaerobic granular sludge through stirring so as to be adsorbed on the surface of the anaerobic granular sludge and then enter the sludge through secretion wrapping to form stable polymers.
10. The method for enhancing anaerobic methanogenesis based on biochar-anaerobic granular sludge as claimed in claim 1, wherein the biochar is further combined with flocculent sludge to form compact granules wrapped with biochar.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116621327A (en) * | 2023-06-12 | 2023-08-22 | 长沙经济技术开发区水质净化工程有限公司 | Method for constructing active heavy mud system by using endogenous carbon source filler |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116621327A (en) * | 2023-06-12 | 2023-08-22 | 长沙经济技术开发区水质净化工程有限公司 | Method for constructing active heavy mud system by using endogenous carbon source filler |
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