CN115747065A - Experimental device for in vitro circulation dosing simulation is to vascular endothelium effect - Google Patents
Experimental device for in vitro circulation dosing simulation is to vascular endothelium effect Download PDFInfo
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- CN115747065A CN115747065A CN202211427625.7A CN202211427625A CN115747065A CN 115747065 A CN115747065 A CN 115747065A CN 202211427625 A CN202211427625 A CN 202211427625A CN 115747065 A CN115747065 A CN 115747065A
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- 230000004087 circulation Effects 0.000 title claims abstract description 10
- 210000003989 endothelium vascular Anatomy 0.000 title claims abstract description 8
- 230000000694 effects Effects 0.000 title abstract description 7
- 238000000338 in vitro Methods 0.000 title 1
- 238000004088 simulation Methods 0.000 title 1
- 239000007788 liquid Substances 0.000 claims abstract description 54
- 230000009471 action Effects 0.000 claims abstract description 12
- 230000005674 electromagnetic induction Effects 0.000 claims abstract description 5
- 239000003814 drug Substances 0.000 abstract description 15
- 229940079593 drug Drugs 0.000 abstract description 12
- 239000008280 blood Substances 0.000 abstract description 9
- 210000004369 blood Anatomy 0.000 abstract description 8
- 210000004204 blood vessel Anatomy 0.000 abstract description 8
- 210000003038 endothelium Anatomy 0.000 abstract description 8
- 210000002966 serum Anatomy 0.000 abstract description 7
- 230000007547 defect Effects 0.000 abstract description 6
- 230000007246 mechanism Effects 0.000 abstract description 4
- 230000033228 biological regulation Effects 0.000 abstract description 3
- 238000011160 research Methods 0.000 description 6
- 238000004113 cell culture Methods 0.000 description 5
- 230000017531 blood circulation Effects 0.000 description 4
- 210000002889 endothelial cell Anatomy 0.000 description 4
- 208000024172 Cardiovascular disease Diseases 0.000 description 3
- 208000026106 cerebrovascular disease Diseases 0.000 description 3
- 230000002526 effect on cardiovascular system Effects 0.000 description 3
- 230000003511 endothelial effect Effects 0.000 description 3
- 230000002792 vascular Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 210000001503 joint Anatomy 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000003032 molecular docking Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- 210000003556 vascular endothelial cell Anatomy 0.000 description 1
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Abstract
The invention discloses an experimental device for simulating the action of extracorporeal circulation dosing on vascular endothelium, which comprises a driving device, a circulating pump, a liquid inlet pipe, a liquid outlet pipe and a culture pipe, wherein two ends of the liquid inlet pipe are respectively butted with a liquid inlet of the circulating pump and a liquid outlet end of the culture pipe; a rotating shaft is arranged in the culture tube, brush rods are uniformly distributed on the shaft body of the rotating shaft, and the end parts of the brush rods contact the inner wall of the culture tube; wherein, the driving unit drives the rotating shaft to rotate around the outside of the culture tube under the action of electromagnetic induction. The invention effectively solves the defects that the existing experimental device can not be used for researching the effect of circulating blood (such as drug-containing serum) on blood vessels (endothelium) in a flowing state, and particularly can not be used for researching the regulation and control mechanism of related drugs on the structure and the function of the blood vessels endothelium along with the blood flowing state.
Description
Technical Field
The invention belongs to the technical field of medicine, and particularly relates to an experimental device for simulating the action of extracorporeal circulation drug delivery on vascular endothelium.
Background
Once this balance is broken, cardiovascular and cerebrovascular diseases and their complications will ensue. The research on the function of vascular endothelial cells in the current basic research has been a hot research focus in the field of cardiovascular and cerebrovascular diseases, but the existing experimental device has the defect that the existing experimental device can not well organically combine the blood flow (biomechanical effect) -medicine (containing pharmaceutical serum) -blood vessels (endothelial cells), so that the existing experimental device is difficult to be used for researching the action and the mechanism of the circulating blood (such as serum containing medicine) on the blood vessels (endothelium) in the flowing state.
Introduction of biomechanical technology and method in the traditional pharmacology field can more comprehensively evaluate pharmacological effects and provide a new research idea for treating cardiovascular and cerebrovascular diseases. However, the prior art does not develop necessary experimental devices specially aiming at such related researches, which greatly limits the research progress of related medicines, especially traditional Chinese medicines (containing pharmaceutical preparations) for promoting blood circulation and removing blood stasis on the vascular endothelial structure and the function regulation mechanism and the like under the condition of the accompanying blood flow.
Disclosure of Invention
In order to solve the defects of the prior art, the invention aims to provide an experimental device for simulating the action of extracorporeal circulation drug administration on vascular endothelium.
The invention is realized in this way, a kind of extracorporeal circulation administers the experimental apparatus which simulates the effect to the endothelium of blood vessel, this apparatus includes driving unit, circulating pump, feed liquor pipe, drain pipe, culture tube, the said feed liquor pipe both ends are butted against the inlet of the circulating pump, culture tube liquid outlet separately, the said drain pipe both ends are butted against circulating pump liquid outlet, culture tube liquid inlet separately, there are valve-controlled liquid outlets on the body of the said feed liquor pipe, there are valve-controlled liquid inlets on the body of the said drain pipe; a rotating shaft is arranged in the culture tube, brush rods are uniformly distributed on the shaft body of the rotating shaft, and the end parts of the brush rods are in contact with the inner wall of the culture tube; the driving unit drives the rotating shaft to rotate around the outside of the culture tube under the action of electromagnetic induction.
Preferably, the device further comprises a transparent viewing dish, the culture tube being placed or fixed in the viewing dish.
Preferably, the joint of the culture tube and the liquid inlet tube and the liquid outlet tube is connected by rotary threads.
Preferably, the driving unit comprises a magnetic field generator and an annular magnet, supporting rods are uniformly distributed on the annular magnet by taking the center of the annular magnet as a center, and rollers are mounted at the end parts of the supporting rods; wherein,
the annular magnet is arranged in the liquid inlet pipe or the liquid outlet pipe, the annular magnet and the culture pipe are coaxial, the center of the support rod is butted with the rotating shaft, and the roller is attached to the inner wall of the liquid inlet pipe or the liquid outlet pipe; the magnetic field generator positioned outside the liquid inlet pipe or the liquid outlet pipe controls the annular magnet to rotate through a magnetic field.
Compared with the defects and shortcomings of the prior art, the invention has the following beneficial effects: the invention effectively solves the defects that the existing experimental device can not be used for researching the effect of circulating blood (such as drug-containing serum) on blood vessels (endothelium) in a flowing state, and particularly can not be used for researching the regulation and control mechanism of related drugs on the structure and the function of the blood vessels endothelium along with the blood flowing state.
Drawings
FIG. 1 is a schematic diagram of the structure of an experimental device for simulating the action of extracorporeal circulation administration on vascular endothelium;
FIG. 2 is a schematic view of the internal cross-sectional configuration of the outlet conduit at the location of the drive unit mounting in the device of FIG. 1;
FIG. 3 is a schematic view showing the internal structure of a section of the culture tube in the apparatus shown in FIG. 1;
fig. 4 is a schematic view of the butt joint structure of the butt joint shaft and the rotating shaft in the device of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not limit the invention.
As shown in fig. 1-4, wherein fig. 1 is a schematic structural diagram of an experimental device for simulating the action of extracorporeal circulation administration on vascular endothelium according to the present invention; FIG. 2 is a schematic view of the internal cross-sectional configuration of the effluent conduit at the location of the drive unit mounting in the apparatus of FIG. 1; FIG. 3 is a schematic view showing the internal structure of a section of a culture tube in the apparatus shown in FIG. 1; fig. 4 is a schematic view of the butt-joint structure of the butt-joint shaft and the rotating shaft in the device of the invention.
The embodiment of the invention discloses an experimental device for simulating the effect of extracorporeal circulation dosing on vascular endothelium, which comprises a driving unit 1, a circulating pump 2, a liquid inlet pipe 3, a liquid outlet pipe 4 and a culture pipe 5, wherein two ends of the liquid inlet pipe 3 are respectively butted with a liquid inlet of the circulating pump 2 and a liquid outlet end of the culture pipe 5, two ends of the liquid outlet pipe 4 are respectively butted with a liquid outlet of the circulating pump 2 and a liquid inlet end of the culture pipe 5, a valve-controlled liquid outlet 6 is arranged on a pipe body of the liquid inlet pipe 3, and a valve-controlled liquid inlet 7 is arranged on a pipe body of the liquid outlet pipe 4; a rotating shaft 8 is arranged in the culture tube 5, brush rods 9 are uniformly distributed on the shaft body of the rotating shaft 8, and the end parts of the brush rods 9 are contacted with the inner wall of the culture tube 5; wherein, the driving unit 1 drives the rotating shaft 8 to rotate around the outside of the culture tube 5 through the action of electromagnetic induction.
In the embodiment of the present invention, in order to provide suitable culture conditions, the apparatus further comprises a cell culture incubator (not shown), wherein the driving unit 1, the circulating pump 2, the liquid inlet pipe 3, the liquid outlet pipe 4 and the culture pipe 5 are disposed in the cell culture incubator. In addition, in order to facilitate the positioning and observation of the culture tubes 5, the device further comprises a transparent observation dish 10, wherein the culture tubes 5 are placed in the observation dish and are conveniently placed in a cell culture box in the later fixing stage.
In the embodiment of the invention, in order to ensure the growth of endothelial cells 11 attached to the inner wall of the culture tube 5, the joint of the culture tube 5 and the liquid inlet tube 3 and the liquid outlet tube 4 is connected by rotating threads. Wherein, the culture tube 5 can be driven to rotate at a relatively slow speed while the driving unit 1 drives the rotating shaft 8 to rotate around the outside of the culture tube 5 through the action of electromagnetic induction. Since the culture tube 5 is placed in the transparent observation vessel 10, the presence or absence of liquid seepage in the tube at the rotary threaded connection can be observed well.
The existing driving means which can achieve the function and purpose of the present invention can be used to explain the driving unit 1 of the present invention. The embodiment of the invention provides a specific implementation manner, specifically, the driving unit 1 comprises a magnetic field generator and an annular magnet 1-1, wherein supporting rods 1-2 uniformly distributed at the center of the annular magnet 1-1 are arranged on the annular magnet 1-1, and rollers 1-3 are arranged at the end parts of the supporting rods 1-2; wherein, the annular magnet 1-1 is arranged in the liquid outlet pipe 4, the annular magnet 1-1 and the culture pipe 5 share the same axis, the center of the support rod 1-2 is butted with the rotating shaft 8, and the roller 1-3 is jointed on the inner wall of the liquid outlet pipe 4; the magnetic field generator positioned outside the liquid inlet pipe 3 or the liquid outlet pipe 4 controls the annular magnet 1-1 to rotate through a magnetic field.
In the embodiment of the present invention, in order to facilitate the detachment and installation of the driving unit 1 and the rotating shaft 8, specifically, a docking shaft 1-4 is fixed at the center of the supporting rod 1-2, and an end of the docking shaft 1-4 is docked with the rotating shaft 8. More specifically, the end of the rotating shaft 8 is hollow, the end of the butt-joint shaft 1-4 is in a pointed design, and the butt-joint is completed by inserting the pointed end of the butt-joint shaft 1-4 into the hollow interior of the end of the rotating shaft 8.
In the practical application process of the invention, after the driving unit 1, the circulating pump 2, the liquid inlet pipe 3, the liquid outlet pipe 4 and the culture pipe 5 are assembled, the culture pipe 5 is placed in the transparent observation dish 10 to be fixed, all the assembled structures are placed in the cell culture incubator, various culture parameters of the cell culture incubator are adjusted, the driving unit 1 is started, cells in endothelial cell 11 suspension are uniformly attached to the inner wall of the red culture pipe 5 along with the rotation of the rotating shaft 8, and a simulated vascular endothelial wall is gradually formed; then, the valve of the inlet 7 on the tube body of the outlet tube 4 is opened, after the prepared drug is injected, the valve of the inlet 7 is closed, the circulating pump 2 is opened, the flow rate of the drug solution is set, and the drug-containing serum or plasma rich in the relevant drug is circulated by the pump (the speed is kept similar to the blood flow rate, so that the influence of dynamic drug serum and plasma on the endothelial cells 11 can be conveniently observed.
The invention effectively solves the defects that the existing experimental device can not be used for researching the effect of circulating blood (such as drug-containing serum) on blood vessels (endothelium) in a flowing state, and particularly can not be used for researching the regulation and control mechanism of related drugs on the structure and the function of the blood vessels endothelium along with the blood flowing state.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (4)
1. An experimental device for simulating the action of extracorporeal circulation dosing on vascular endothelium is characterized by comprising a driving device, a circulating pump, a liquid inlet pipe, a liquid outlet pipe and a culture pipe, wherein two ends of the liquid inlet pipe are respectively butted with a liquid inlet of the circulating pump and a liquid outlet end of the culture pipe; a rotating shaft is arranged in the culture tube, brush rods are uniformly distributed on the shaft body of the rotating shaft, and the end parts of the brush rods contact the inner wall of the culture tube; the driving unit drives the rotating shaft to rotate around the outside of the culture tube under the action of electromagnetic induction.
2. The device of claim 1, further comprising a transparent viewing dish, wherein the culture tube is placed or secured in the viewing dish.
3. The experimental device as claimed in claim 1, wherein the joint of the culture tube and the liquid inlet tube and the liquid outlet tube is connected by a rotary screw.
4. The experimental device as claimed in claim 1, wherein the driving unit comprises a magnetic field generator and a ring magnet, the ring magnet is provided with support rods uniformly distributed at the center of the ring magnet, and the ends of the support rods are provided with rollers; wherein,
the annular magnet is arranged in the liquid inlet pipe or the liquid outlet pipe, the annular magnet and the culture pipe are coaxial, the center of the support rod is butted with the rotating shaft, and the roller is attached to the inner wall of the liquid inlet pipe or the liquid outlet pipe; the magnetic field generator positioned outside the liquid inlet pipe or the liquid outlet pipe controls the annular magnet to rotate through a magnetic field.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211427625.7A CN115747065A (en) | 2022-11-15 | 2022-11-15 | Experimental device for in vitro circulation dosing simulation is to vascular endothelium effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211427625.7A CN115747065A (en) | 2022-11-15 | 2022-11-15 | Experimental device for in vitro circulation dosing simulation is to vascular endothelium effect |
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| Publication Number | Publication Date |
|---|---|
| CN115747065A true CN115747065A (en) | 2023-03-07 |
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| CN202211427625.7A Pending CN115747065A (en) | 2022-11-15 | 2022-11-15 | Experimental device for in vitro circulation dosing simulation is to vascular endothelium effect |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1615994A (en) * | 2004-09-28 | 2005-05-18 | 南京中医药大学 | Method for preparing Chinese medicine composition for treating ischemic brain apoplexy |
| CN101333498A (en) * | 2008-06-13 | 2008-12-31 | 东华大学 | Apparatus for culturing tissue engineered blood |
| CN101372661A (en) * | 2008-03-18 | 2009-02-25 | 北京航空航天大学 | Adjustable pouring type vascular tissue engineering reactor having rotating cultivation cavity |
| CN102127507A (en) * | 2010-12-15 | 2011-07-20 | 中国人民解放军第四军医大学 | Biological tissue engineering vasculogenesis system |
| CN201915100U (en) * | 2010-12-15 | 2011-08-03 | 中国人民解放军第四军医大学 | Blood vessel generator of biological tissue engineering |
| CN203561634U (en) * | 2013-08-06 | 2014-04-23 | 李钊伦 | In-vitro blood state simulator |
| CN104146794A (en) * | 2014-08-24 | 2014-11-19 | 周惠兴 | Blood vessel forming device and method for 3D bioprinting |
| CN211847987U (en) * | 2020-01-10 | 2020-11-03 | 中国人民解放军第四军医大学 | Endothelial cell culture apparatus |
| US20210222130A1 (en) * | 2018-08-07 | 2021-07-22 | 1Lab Sa | Model for in-vitro simulation of the behaviour of dysfunctional vessels |
-
2022
- 2022-11-15 CN CN202211427625.7A patent/CN115747065A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1615994A (en) * | 2004-09-28 | 2005-05-18 | 南京中医药大学 | Method for preparing Chinese medicine composition for treating ischemic brain apoplexy |
| CN101372661A (en) * | 2008-03-18 | 2009-02-25 | 北京航空航天大学 | Adjustable pouring type vascular tissue engineering reactor having rotating cultivation cavity |
| CN101333498A (en) * | 2008-06-13 | 2008-12-31 | 东华大学 | Apparatus for culturing tissue engineered blood |
| CN102127507A (en) * | 2010-12-15 | 2011-07-20 | 中国人民解放军第四军医大学 | Biological tissue engineering vasculogenesis system |
| CN201915100U (en) * | 2010-12-15 | 2011-08-03 | 中国人民解放军第四军医大学 | Blood vessel generator of biological tissue engineering |
| CN203561634U (en) * | 2013-08-06 | 2014-04-23 | 李钊伦 | In-vitro blood state simulator |
| CN104146794A (en) * | 2014-08-24 | 2014-11-19 | 周惠兴 | Blood vessel forming device and method for 3D bioprinting |
| US20210222130A1 (en) * | 2018-08-07 | 2021-07-22 | 1Lab Sa | Model for in-vitro simulation of the behaviour of dysfunctional vessels |
| CN211847987U (en) * | 2020-01-10 | 2020-11-03 | 中国人民解放军第四军医大学 | Endothelial cell culture apparatus |
Non-Patent Citations (1)
| Title |
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