CN115517999A

CN115517999A - Anti-aging composition and application thereof in beauty and skin care

Info

Publication number: CN115517999A
Application number: CN202211319447.6A
Authority: CN
Inventors: 雷楚屏
Original assignee: Guangzhou Aizhuo Biotechnology Co ltd
Current assignee: Guangzhou Aizhuo Biotechnology Co ltd
Priority date: 2022-10-26
Filing date: 2022-10-26
Publication date: 2022-12-27
Anticipated expiration: 2042-10-26
Also published as: CN115517999B

Abstract

The invention discloses an anti-aging composition and application thereof in beauty treatment and skin care, wherein the anti-aging composition comprises the following raw materials in parts by mass: 0.1-5 parts of antioxidant, 0.0001-0.05 part of composite polypeptide, 1-10 parts of panthenol and 0.3-2 parts of glucoside compound; the antioxidant is at least one selected from arbutin, ergothioneine, grape seed extract, olive leaf extract, glabridin, retinol and its derivatives, and ascorbic acid and its derivatives; the composite polypeptide is at least one selected from arginine/lysine polypeptide, acetyl octapeptide-3, acetyl hexapeptide-8, pentapeptide-3, tripeptide-1 and acetyl hexapeptide-1; the glycoside compound is at least one selected from glycerol glucoside, glucopyranoside, dextran and sodium hyaluronate. The cosmetic prepared from the anti-aging composition is matched with a radio frequency cosmetic instrument for use, and can promote the regeneration of dermal collagen of skin, fill and fade fine lines, and achieve the effects of deep anti-aging, long-acting moisturizing, anti-wrinkle and skin nourishing.

Description

Anti-aging composition and application thereof in beauty and skin care

Technical Field

The invention belongs to the field of cosmetics, and particularly relates to an anti-aging composition and application thereof in beauty and skin care.

Background

In the field of new medical beauty and skin care, radio frequency is a beauty technology which is eagerly pursued by market consumers in recent years. Radio frequency utilizes the switching of the polarity of an electric field to produce a thermal effect on the friction of the tissue through which the current passes, and since the conductivity of the dermis is highest in skin tissue, the current is mainly concentrated in the dermis layer, causing bulk volumetric heating of the dermis tissue. When the heating temperature reaches 60 ℃, the collagen is instantly contracted, and when the heating temperature reaches 42 ℃, the conformation of the collagen helical structure is changed, so that the self wound repair mechanism of an organism can be further started, fibroblasts are stimulated to generate new collagen, elasticity and synthesis of other supporting proteins, and the effects of tightening the skin, removing wrinkles, lifting, improving the skin quality and the outline and the like are achieved.

The objective of careful research on parameters such as radio frequency, instrument efficacy and radio frequency depth of action is to apply faster and greater energy to the dermis, but too high energy easily causes thermal damage to healthy cells and surrounding tissue cells, generates oxidative free radicals, and even directly affects the normal metabolism of the skin. In the case of hot marie radio frequency, the customer usually experiences a dry, red, swollen, scab-forming repair period of about 3-10 days after surgery.

In addition, the mechanism of action of the radiofrequency cosmetic device determines that fibroblasts in the dermis synthesize more extracellular matrix, but the newly synthesized extracellular matrix is not uniformly distributed. The reason for this is that fibroblasts are living and can sense and respond to mechanical force, and secreted collagen fibers can form stronger fiber bundles along the direction of the mechanical force to resist the mechanical force. The force non-uniformity is increasingly manifested, and the characters are commonly called Chinese, crow's feet, raised head, etc. As the age increases, wrinkles become more and more obvious, so that the problem of collagen arrangement is not solved only by using the radio frequency beauty instrument, but also the improvement effect on the wrinkles such as expression lines is limited.

Therefore, the anti-aging composition with good moisturizing, anti-oxidation and neurotransmitter conduction inhibiting effects is of great significance for exerting the anti-aging and beautifying effects of the radio frequency beautifying instrument.

Disclosure of Invention

In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide an anti-aging composition. The anti-aging composition provided by the invention adopts safe and non-toxic raw materials, has no side effect and is mild in formula. The anti-wrinkle composition is obtained by compounding the antioxidant, the composite polypeptide, the panthenol and the glycoside compound in a specific ratio, the anti-wrinkle performance of the composition can be remarkably improved, and cosmetics prepared from the anti-aging composition have the effects of resisting oxidation, preserving moisture for a long time, resisting aging, resisting bacteria and the like. The prepared cosmetic is used in combination with a radio frequency beauty instrument, can promote the regeneration of dermal collagen of skin, fill and fade fine lines, and achieve the effects of deep anti-aging, long-acting moisturizing, anti-wrinkle and skin nourishing.

The invention aims to provide an anti-aging composition, which comprises the following raw materials in parts by mass:

the antioxidant is at least one selected from arbutin, ergothioneine, grape seed extract, olive leaf extract, glabridin, retinol and its derivatives, and ascorbic acid and its derivatives;

the composite polypeptide is selected from at least one of arginine/lysine polypeptide, acetyl octapeptide-3, acetyl hexapeptide-8, pentapeptide-3, tripeptide-1 and acetyl hexapeptide-1;

the glycoside compound is at least one selected from glycerol glucoside, glucopyranoside, dextran and sodium hyaluronate.

Arbutin is also called arbutin, and is widely existed in plants of multiple families, easy to extract, artificially synthesized and low in toxicity. In cytological experiments, cell morphology observation and biochemical index detection are carried out, and RT-PCR is adopted to verify the relation between the arbutin and Grx-mRNA expression, so that arbutin has certain antioxidation, and meanwhile, the arbutin also has the effects of inhibiting the activity of tyrosinase in vivo, preventing the generation of melanin and sterilizing and diminishing inflammation.

Ergothioneine is a natural antioxidant, can protect cells in human body, is an important active substance in organism, and has multiple physiological functions of scavenging free radicals, whitening skin, resisting aging, preventing ultraviolet radiation damage, maintaining DNA synthesis and normal cell growth, etc.

The grape seed extract is a novel high-efficiency natural antioxidant substance which can not be synthesized in a human body and is extracted from grape seeds. It can effectively remove redundant free radicals in human body, and has the functions of delaying senility and enhancing immunity. Has effects of resisting oxidation, resisting allergy, relieving fatigue, improving body condition, improving sub-health state, delaying aging, improving dysphoria, irascibility, dizziness, debilitation, hypomnesis, etc.

The olive leaves contain triterpenes, flavonoids, iridoids, free alcohol, sterols, alkanes, chalcones and other components, and the olive leaf extract mainly comprises oleuropein, hydroxytyrosol and other effective components, wherein the oleuropein is a nontoxic secoiridoid glycoside compound. The study showed that. Oleuropein has antifungal, antiinflammatory, antiviral, antioxidant, anticancer, and blood glucose reducing effects. Oleuropein is also the component with the strongest biological activity, has strong antioxidant activity in vitro, and the strong antioxidant activity comes from the structure of adjacent dihydroxy substitution, and can be combined with superoxide anion, hydrogen peroxide, nitric oxide free radical and nitro free radical to eliminate free radical. Hydroxytyrosol has a catechol structure, is a typical antioxidant stress substance and free radical scavenger, and has an ORAC antioxidant adsorption free radical energy value which is more than 10 times higher than that of vitamin C and more than 4-6 times higher than that of anthocyanin according to an authoritative data of a Dalelix laboratory in Boston, mass.

Glabridin has antioxidant and antiaging effects, is mainly present at root and stem of Glycyrrhiza glabra Linne, has strong ability of scavenging free radicals, and has skin whitening effect, but easily deteriorates in high temperature environment. In addition, the glabridin also has a certain anti-inflammatory effect, and has anti-inflammatory and whitening effects on skin pigmentation after the glabridin is used persistently.

The retinol can help the skin stratum corneum to restore normal metabolism, thicken the stratum corneum of the skin, enhance the water storage capacity of the skin and promote the generation of collagen in the dermis of the skin; has effects in refining pores, resisting aging, removing wrinkle, removing acne, and whitening skin.

Ascorbic acid and its derivatives are highly effective antioxidants, and because ascorbic acid is easily oxidized to produce dehydroascorbic acid when exposed to air or aqueous solutions, more stable ascorbic acid derivatives such as magnesium ascorbyl phosphate, ascorbyl tetraisopalmitate, ascorbyl ethyl ether, etc. are commonly used in cosmetics to generate ascorbic acid after enzymatic hydrolysis in skin, which plays a role in assisting in synthesizing collagenase, photoprotecting, inhibiting tyrosinase, and resisting photoaging.

Glycerol glucosides are glycoside compounds formed by connecting glycerol molecules and glucose molecules through glycosidic bonds, wherein the structure with important physiological functions is 2-alpha configuration. The glycerol glucoside is an emergency product of plants under extreme conditions, is favorable for protecting the integrity of cell membranes under the extreme conditions, and can promote the expression of skin aquaporin AQP3, enhance the moisture retention capacity of skin, promote the growth of skin cells, accelerate cell repair and metabolism, resist inflammation and the like in skin care products.

Glucopyranoside is a polycyclic structure with the following structural formula:

the dextran refers to homotype polysaccharide composed of glucose as monosaccharide, and glucose units are connected by glycosidic bonds.

The sodium hyaluronate belongs to a high molecular polymer and has strong lubricating feeling and film forming property, so that the skin care product containing the sodium hyaluronate has obvious lubricating feeling and good hand feeling when being smeared. The macromolecular sodium hyaluronate forms a layer of breathable film on the surface of the skin, so that the skin is smooth and moist, and the invasion of external bacteria, dust and the like is blocked; sodium hyaluronate with small molecular weight can permeate into dermis, and has effects of slightly dilating capillary, increasing blood circulation, improving metabolism, promoting skin nutrition absorption and waste excretion, preventing skin aging, and caring skin.

Panthenol, also known as panthenol, is a vitamin B5, colorless to yellowish, clear, viscous liquid. Panthenol has effects of preventing pachylosis and aging. Also has obvious anti-inflammatory effect, can delay the progress of inflammatory reaction, and is an ideal auxiliary anti-acne component for sensitive muscles or varicella muscles.

Preferably, the anti-aging composition comprises the following raw materials in parts by mass:

the composite polypeptide is at least one selected from arginine/lysine polypeptide, acetyl octapeptide-3, acetyl hexapeptide-8, pentapeptide-3, tripeptide-1 and acetyl hexapeptide-1;

More preferably, the anti-aging composition comprises the following raw materials in parts by mass:

Preferably, the extraction process of the olive leaf extract comprises the following steps:

pulverizing Olea europaea leaf, adding water to the pulverized material to extract water soluble components, filtering, centrifuging, collecting supernatant, performing chromatography, concentrating at low temperature, and drying to obtain Olea europaea leaf extract powder.

Preferably, the anti-aging composition also comprises 1-20 parts of polyhydroxy moisturizer with the carbon chain length of C3-C6.

More preferably, the anti-aging composition further comprises 10 parts of a polyhydroxy moisturizer with a carbon chain length of C3-C6.

Preferably, the polyhydroxy humectant with the carbon chain length of C3-C6 is at least one selected from glycerol, propylene glycol, butanediol, methyl propylene glycol, rhamnose, erythritol, arabinose and saccharide isomer.

More preferably, the polyhydroxy humectant with the carbon chain length of C3-C6 is selected from at least one of glycerol and rhamnose.

The glycerin selected by the invention has good water retention capacity and skin surface permeability; rhamnose has effects of promoting keratinocyte cell proliferation and promoting hemidesmosome structure. Hemidesmosome is located primarily on the basal surface of the epithelial cells and serves to link the epithelial cells to the underlying basement membrane, thus enhancing the barrier function of the skin.

The invention also aims to provide application of the anti-aging composition in preparing cosmetics, wherein the cosmetics comprise the anti-aging composition, and the cosmetics are selected from any one of facial mask liquid, emulsion, cream, toner, essence, stock solution, cleansing milk, lotion, perfume, makeup remover, foundation solution, foundation cream and concealer.

Preferably, the anti-aging composition is used in an amount of 5-40% by weight of the cosmetic quality.

Still another object of the present invention is to provide the use of the anti-aging composition in radio frequency cosmetic and medical cosmetic skin care.

When the skin care product is used singly, the active ingredients of the skin care product are usually blocked by the skin barrier of a human body, most of the active ingredients stay in the horny layer of the epidermis and hardly permeate through the epidermis layer, or the concentration of the active ingredients permeating into the dermis layer of the skin is too low, so that fibroblasts are not stimulated to generate more collagen, elastic fibers and extracellular matrix, the thickness of the dermis layer is not changed obviously, and the anti-oxidation and anti-aging effects are very limited. The anti-aging composition is applied to radio frequency beauty and skin care, energy generated by a radio frequency instrument stimulates a dermis layer to generate more extracellular matrix, and meanwhile, the hydration degree of the epidermis layer can be accelerated, the osmotic pressure of a contact surface is increased, and the transdermal absorption of active ingredients of cosmetics is promoted.

Compared with the prior art, the invention has the following beneficial effects:

(1) The composite polypeptide selected by the invention blocks a muscle fiber sodium ion inflow channel Nav1.4, so that the sodium ion inflow is blocked, the calcium ion inflow is regulated, the muscle operation potential cannot be formed, and further the muscle contraction is weakened, meanwhile, the N-terminal end of the SANP-25 is simulated, and participates in competition of SNAP-25 at the site of a vacuole complex, so that the formation of the complex is influenced, the conduction path of neurotransmitter is finally inhibited/blocked, and further, dynamic expression lines are prevented and removed, and the depth and density of wrinkles are reduced.

(2) According to the invention, the anti-wrinkle composition is obtained by compounding the antioxidant, the composite polypeptide, the panthenol and the glycoside compound in a specific ratio, so that the anti-wrinkle performance of the composition can be obviously improved, and the cosmetics prepared from the anti-wrinkle composition have the effects of resisting oxidation, preserving moisture for a long time, stretching wrinkles and the like.

(3) The cosmetic prepared from the antioxidant, the composite polypeptide, the panthenol and the glycoside compound in a specific ratio is matched with a radio frequency beauty instrument for use, and can promote the regeneration of dermal collagen of skin, fill and fade fine wrinkles, and achieve the effects of deep anti-aging, long-acting moisture retention, wrinkle resistance and skin nourishing.

(4) The anti-aging composition adopted by the invention has the advantages of wide raw material source, safety, no toxicity, no side effect and mild formula.

Detailed Description

In order to make those skilled in the art better understand the technical solution of the present invention, the following will clearly and completely describe the technical solution of the present invention with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, shall fall within the protection scope of the present invention.

Grape seed extract (grape seed procyanidin greater than 95%) was purchased from Guilin Rhein Biotech GmbH

Example 1: the extraction process of the olive leaf extract comprises the following steps:

crushing olive leaves by using a crusher to break the wall, adding deionized water with the mass being 3-5 times of that of the crushed olive leaves to extract water-soluble components, filtering the crushed olive leaves by three layers, centrifuging the filtrate at a high speed, taking supernatant, performing resin chromatography to obtain a primary olive leaf extract product, concentrating the primary olive leaf extract product at a low temperature, drying and spraying the powder to obtain olive leaf extract powder.

Example 2: preparation of an anti-aging composition.

Uniformly mixing 0.2g of arbutin, 0.0005g of arginine/lysine polypeptide, 0.0005g of acetyl hexapeptide, 0.1g of glycerol glucoside, 0.5g of panthenol and 1g of glycerol to obtain the anti-aging composition.

Example 3: preparation of an anti-aging composition.

Mixing ergothioneine 0.2g, acetyl hexapeptide-1.001g, glucopyranoside 0.1g, panthenol 0.5g, and glycerol 1g to obtain antiaging composition.

Example 4: preparation of antiaging composition.

Uniformly mixing 0.2g of glabridin, 0.001g of acetyl octapeptide, 0.1g of glucan, 0.5g of panthenol and 1g of glycerol to obtain the anti-aging composition.

Example 5: preparation of an anti-aging composition.

Uniformly mixing glabridin 0.2g, acetyl octapeptide-3.0003g, acetyl hexapeptide-1.0007g, sodium hyaluronate 0.1g, panthenol 0.5g and glycerol 1g to obtain the anti-aging composition.

Example 6: preparation of an anti-aging composition.

Mixing grape seed extract 0.2g, acetyl octapeptide-3.0006 g, acetyl hexapeptide-1.0004g, glycerol glucoside 0.1g, panthenol 0.5g, and glycerol 1g uniformly to obtain the antiaging composition.

Example 7: preparation of an anti-aging composition.

Mixing Olea europaea leaf extract 0.2g, acetyl octapeptide-3.0004g, acetyl hexapeptide-1.0006 g, glycerol glucoside 0.1g, panthenol 0.5g, and glycerol 1g uniformly to obtain antiaging composition.

Example 8: preparation of an anti-aging composition.

Mixing retinol 0.0062g, acetyl octapeptide-3.0002g, acetyl hexapeptide-1.0008g, glycerol glucoside 0.1038g, panthenol 0.69g, and glycerol 1g to obtain the antiaging composition.

Example 9: preparation of antiaging composition.

Mixing 0.2g ascorbic acid ethyl ether, 0.0007g acetyl hexapeptide, 0.0003g acetyl hexapeptide, 0.1g glycerol glucoside, 0.5g panthenol and 1g glycerol uniformly to obtain the antiaging composition.

Example 10: preparation of an anti-aging composition.

Mixing ergothioneine 0.2g, tripeptide-1.001g, dextran 0.06g, glycerol glucoside 0.04g, panthenol 0.5g, and glycerol 1g to obtain antiaging composition.

Example 11: preparation of antiaging composition.

Mixing Olea europaea leaf extract 0.1g, glabridin 0.1g, pentapeptide-3.001g, glycerol glucoside 0.1g, panthenol 0.5g, and glycerol 1g to obtain antiaging composition.

Comparative example 1: preparation of an anti-aging composition.

And uniformly mixing 0.2g of arbutin and 1.601g of glycerol to obtain the anti-aging composition.

Comparative example 2: preparation of an anti-aging composition.

Uniformly mixing 0.2g of arbutin, 0.0005g of arginine/lysine polypeptide, 0.0005g of acetyl hexapeptide, 0.1g of glycerol glucoside and 1.5g of glycerol to obtain the anti-aging composition.

Comparative example 3: preparation of an anti-aging composition.

0.2g of arbutin, 0.1g of glycerol glucoside, 0.5g of panthenol and 1.001g of glycerol are mixed uniformly to obtain the anti-aging composition.

Comparative example 4: preparation of an anti-aging composition.

Uniformly mixing 0.2g of arbutin, 0.0005g of arginine/lysine polypeptide, 0.0005g of acetyl hexapeptide, 0.5g of panthenol and 1.1g of glycerol to obtain the anti-aging composition.

Comparative example 5: preparation of antiaging composition.

0.2g of ergothioneine and 1.601g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 6: preparation of an anti-aging composition.

Mixing ergothioneine 0.2g, acetyl hexapeptide-1.001g, glucopyranoside 0.1g, and glycerol 1.5g to obtain the antiaging composition.

Comparative example 7: preparation of an anti-aging composition.

0.2g of ergothioneine, 0.001g of acetyl hexapeptide, 0.5g of panthenol and 1.1g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 8: preparation of an anti-aging composition.

0.2g of ergothioneine, 0.1g of glucopyranoside, 0.5g of panthenol and 1.001g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 9: preparation of antiaging composition.

And uniformly mixing 0.2g of glabridin and 1.601g of glycerol to obtain the anti-aging composition.

Comparative example 10: preparation of antiaging composition.

Uniformly mixing 0.2g of glabridin, 0.001g of acetyl octapeptide, 0.1g of glucan and 1.5g of glycerol to obtain the anti-aging composition.

Comparative example 11: preparation of an anti-aging composition.

Uniformly mixing 0.2g of glabridin, 0.001g of acetyl octapeptide, 0.5g of panthenol and 1.1g of glycerol to obtain the anti-aging composition.

Comparative example 12: preparation of an anti-aging composition.

And uniformly mixing 0.2g of glabridin, 0.1g of glucan, 0.5g of panthenol and 1.001g of glycerol to obtain the anti-aging composition.

Comparative example 13: preparation of antiaging composition.

Uniformly mixing 0.2g of glabridin, 0.0003g of acetyl octapeptide, 0.0007g of acetyl hexapeptide, 0.1g of sodium hyaluronate and 1.5g of glycerol to obtain the anti-aging composition.

Comparative example 14: preparation of an anti-aging composition.

Mixing glabridin 0.2g, acetyl octapeptide-3.0003 g, acetyl hexapeptide-1.0007 g, panthenol 0.5g, and glycerol 1.1g uniformly to obtain antiaging composition.

Comparative example 15: preparation of an anti-aging composition.

Uniformly mixing 0.2g of glabridin, 0.1g of sodium hyaluronate, 0.5g of panthenol and 1.001g of glycerol to obtain the anti-aging composition.

Comparative example 16: preparation of antiaging composition.

And uniformly mixing 0.2g of grape seed extract and 1.601g of glycerol to obtain the anti-aging composition.

Comparative example 17: preparation of an anti-aging composition.

Mixing grape seed extract 0.2g, acetyl octapeptide-3.0006 g, acetyl hexapeptide-1.0004g, glycerol glucoside 0.1g, and glycerol 1.5g to obtain the antiaging composition.

Comparative example 18: preparation of antiaging composition.

Mixing grape seed extract 0.2g, acetyl octapeptide-3.0006 g, acetyl hexapeptide-1.0004g, panthenol 0.5g, and glycerol 1.1g to obtain antiaging composition.

Comparative example 19: preparation of an anti-aging composition.

Mixing grape seed extract 0.2g, glycerol glucoside 0.1g, panthenol 0.5g, and glycerol 1.001g to obtain antiaging composition.

Comparative example 20: preparation of antiaging composition.

Mixing 0.2g of olive leaf extract and 1.601g of glycerin uniformly to obtain the anti-aging composition.

Comparative example 21: preparation of antiaging composition.

Mixing Olea europaea leaf extract 0.2g, acetyl octapeptide-3.0004 g, acetyl hexapeptide-1.0006 g, glycerol glucoside 0.1g, and glycerol 1.5g uniformly to obtain the antiaging composition.

Comparative example 22: preparation of an anti-aging composition.

Mixing Olea europaea leaf extract 0.2g, acetyl octapeptide-3.0004g, acetyl hexapeptide-1.0006 g, panthenol 0.5g, and glycerol 1.1g to obtain antiaging composition.

Comparative example 23: preparation of an anti-aging composition.

Mixing Olea europaea leaf extract 0.2g, glycerol glucoside 0.1g, panthenol 0.5g, and glycerol 1.001g uniformly to obtain antiaging composition.

Comparative example 24: preparation of an anti-aging composition.

0.0062g of retinol and 1.7948g of glycerol are mixed evenly to obtain the anti-aging composition.

Comparative example 25: preparation of an anti-aging composition.

Mixing retinol 0.0062g, acetyl octapeptide-3.0002g, acetyl hexapeptide-1.0008g, glycerol glucoside 0.1038g, and glycerol 1.69g to obtain the antiaging composition.

Comparative example 26: preparation of antiaging composition.

Mixing retinol 0.0062g, acetyl octapeptide-3.0002g, acetyl hexapeptide-1.0008g, panthenol 0.69g, and glycerol 1.1038g to obtain the antiaging composition.

Comparative example 27: preparation of an anti-aging composition.

Mixing retinol 0.0062g, glycerol glucoside 0.1038g, panthenol 0.69g, and glycerol 1.001g to obtain antiaging composition.

Comparative example 28: preparation of an anti-aging composition.

0.2g of ascorbic acid ethyl ether and 1.601g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 29: preparation of antiaging composition.

Mixing 0.2g ascorbic acid ethyl ether, 0.0007g acetyl hexapeptide-8.0007g acetyl hexapeptide-1.0003g glycerol glucoside 0.1g and glycerol 1.5g to obtain the antiaging composition.

Comparative example 30: preparation of antiaging composition.

Mixing 0.2g of ascorbic acid ethyl ether, 0.0007g of acetyl hexapeptide, 0.0003g of acetyl hexapeptide, 0.5g of panthenol and 1.1g of glycerol uniformly to obtain the anti-aging composition.

Comparative example 31: preparation of antiaging composition.

0.2g of ascorbic acid ethyl ether, 0.1g of glycerol glucoside, 0.5g of panthenol and 1.001g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 32: preparation of an anti-aging composition.

Mixing ergothioneine 0.2g, tripeptide-1.001g, dextran 0.06g, glycerol glucoside 0.04g, and glycerol 1.5g to obtain the antiaging composition.

Comparative example 33: preparation of an anti-aging composition.

0.2g of ergothioneine, 0.001g of tripeptide-1.001g, 0.5g of panthenol and 1.1g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 34: preparation of antiaging composition.

Mixing ergothioneine 0.2g, dextran 0.06g, glycerol glucoside 0.04g, panthenol 0.5g, and glycerol 1.001g to obtain antiaging composition.

Comparative example 35: preparation of an anti-aging composition.

Mixing Olea europaea leaf extract 0.1g, glabridin 0.1g, pentapeptide-3.001g, glycerol glucoside 0.1g, and glycerol 1.5g uniformly to obtain the antiaging composition.

Comparative example 36: preparation of an anti-aging composition.

Mixing Olea europaea leaf extract 0.1g, glabridin 0.1g, pentapeptide-3.001g, panthenol 0.5g, and glycerol 1.1g uniformly to obtain the antiaging composition.

Comparative example 37: preparation of antiaging composition.

Mixing Olea europaea leaf extract 0.1g, glabridin 0.1g, glycerol glucoside 0.1g, panthenol 0.5g, and glycerol 1.001g uniformly to obtain antiaging composition.

Determination of DPPH radical scavenging Capacity:

the testing steps are as follows: a certain amount of DPPH is weighed and prepared into 0.1mg/mL DPPH solution by using absolute ethyl alcohol. 2mL of the anti-aging compositions of examples 2 to 11 and comparative examples 1 to 37 were added to 1000g of a sample solution diluted with absolute ethanol and 2mL of a DPPH solution, mixed uniformly, reacted at room temperature for 30min, and then the absorbance Ai was measured at 517 nm. The absorbance A0 of 2mL of DPPH solution +2mL of absolute ethanol and the absorbance Aj of 2mL of sample solution +2mL of absolute ethanol after mixing were measured at the same time. The assay was repeated 2 times. The DPPH radical clearance rate of the sample is calculated by the following formula: the results are shown in Table 1.

DPPH radical scavenging Rate = (A0-Aj + Ai)/A0X 100%

The evaluation method comprises the following steps: the antioxidant capacity can be reflected by the measurement of DPPH free radical scavenging capacity. The higher the DPPH free radical clearance rate, the stronger the antioxidant capacity, and vice versa.

TABLE 1 DPPH radical scavenging test results for anti-aging compositions of examples 2-11 and comparative examples 1-37.

Sample (I)	Example 2	Comparative example 1	Comparative example 2	Comparative example 3	Comparative example 4
						DPPH radical scavenging ratio (%)	68.7	52.5	55.3	54.7	54.2
Sample (I)	Example 3	Comparative example 5	Comparative example 6	Comparative example 7	Comparative example 8
						DPPH radical scavenging ratio (%)	72.3	58.1	59.4	60.8	59.5
Sample(s)	Example 4	Comparative example 9	Comparative example 10	Comparative example 11	Comparative example 12
						DPPH radical scavenging ratio (%)	50.9	41.2	42.6	43.7	42.9
Sample(s)	Example 5	Comparative example 9	Comparative example 13	Comparative example 14	Comparative example 15
						DPPH radical scavenging Rate (%)	49.4	41.2	43.5	44.9	43.0
Sample(s)	Example 6	Comparative example 16	Comparative example 17	Comparative example 18	Comparative example 19
						DPPH radical scavenging Rate (%)	98.2	84.6	87.7	88.1	88.4
Sample (I)	Example 7	Comparative example 20	Comparative example 21	Comparative example 22	Comparative example 23
						DPPH radical scavenging Rate (%)	99.3	86.3	87.2	87.4	88.1
Sample (I)	Example 8	Comparative example 24	Comparative example 25	Comparative example 26	Comparative example 27
						DPPH radical scavenging Rate (%)	93.4	77.8	79.5	80.1	80.4
Sample (I)	Example 9	Comparative example 28	Comparative example 29	Comparative example 30	Comparative example 31
						DPPH radical scavenging Rate (%)	96.0	79.3	81.7	82.0	82.5
Sample (I)	Example 10	Comparative example 5	Comparative example 32	Comparative example 33	Comparative example 34
						DPPH radical scavenging Rate (%)	71.9	58.1	59.3	60.7	60.0
Sample (I)	Example 11	Comparative example 20	Comparative example 35	Comparative example36	Comparative example 37
						DPPH radical scavenging ratio (%)	99.7	86.3	86.8	87.6	88.2

Arginine/lysine polypeptide, acetyl octapeptide-3, acetyl hexapeptide-8, pentapeptide-3, tripeptide-1, acetyl hexapeptide-1, glycerol glucoside, glucopyranoside, dextran, sodium hyaluronate, panthenol, glycerol, propylene glycol, butylene glycol, methyl propylene glycol, rhamnose, erythritol, arabinose, and saccharide isomers were all tested for their ability to scavenge DPPH radicals.

As can be seen from Table 1, by comparing example 2 with comparative examples 1 to 4, example 3 with comparative examples 5 to 8, example 4 with comparative examples 9 to 12, example 5 with comparative examples 9 and 13 to 15, example 6 with comparative examples 16 to 19, example 7 with comparative examples 20 to 23, example 8 with comparative examples 24 to 27, example 9 with comparative examples 28 to 31, example 10 with comparative examples 5 and 32 to 34, and example 11 with comparative examples 20 and 35 to 37, it can be found that the combination of the composite polypeptide, the panthenol and the glycoside compound in a specific ratio can promote the antioxidant effect of the antioxidant, and the combination of any two of the composite polypeptide, the panthenol and the glycoside compound can not effectively promote the antioxidant effect of the antioxidant. Meanwhile, the compounding of the compound polypeptide, the panthenol and the glycoside compound outside the specific proportion of the invention can not effectively promote the antioxidant effect of the antioxidant. The antioxidant, the composite polypeptide, the panthenol and the glycoside compound in a specific ratio are mixed with other moisturizing agents in the invention, and the obtained anti-aging composition has the action and effect consistent with those of the examples 2-11.

Comparative example 38: preparation of antiaging composition.

And uniformly mixing 0.0801g of arbutin and 0.1g of glycerol to obtain the anti-aging composition.

Comparative example 39: preparation of an anti-aging composition.

0.0801g of ergothioneine and 0.1g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 40: preparation of an anti-aging composition.

And uniformly mixing 0.0801g of grape seed extract and 0.1g of glycerol to obtain the anti-aging composition.

Comparative example 41: preparation of antiaging composition.

Mixing Olea europaea leaf extract 0.0801g and glycerol 0.1g to obtain antiaging composition.

Comparative example 42: preparation of an anti-aging composition.

And uniformly mixing 0.0801g of glabridin and 0.1g of glycerol to obtain the anti-aging composition.

Comparative example 43: preparation of an anti-aging composition.

Uniformly mixing 0.0062g of retinol and 0.00774g of glycerol to obtain the anti-aging composition.

Comparative example 44: preparation of antiaging composition.

0.0801g of ascorbic acid and 0.1g of glycerol are mixed uniformly to obtain the anti-aging composition.

Comparative example 45: preparation of an anti-aging composition.

And uniformly mixing 0.0801g of arginine/lysine polypeptide and 0.1g of glycerol to obtain the anti-aging composition.

Comparative example 46: preparation of antiaging composition.

And uniformly mixing 0.0801g of acetyl octapeptide and 0.1g of glycerol to obtain the anti-aging composition.

Comparative example 47: preparation of an anti-aging composition.

And uniformly mixing 0.0801g of acetyl hexapeptide and 0.1g of glycerol to obtain the anti-aging composition.

Comparative example 48: preparation of an anti-aging composition.

And uniformly mixing 0.0801g of pentapeptide and 0.1g of glycerol to obtain the anti-aging composition.

Comparative example 49: preparation of an anti-aging composition.

And uniformly mixing tripeptide-1.0801g and glycerol 0.1g to obtain the anti-aging composition.

Comparative example 50: preparation of an anti-aging composition.

Comparative example 51: preparation of an anti-aging composition.

And uniformly mixing 0.0801g of glycerol glucoside and 0.1g of glycerol to obtain the anti-aging composition.

Comparative example 52: preparation of an anti-aging composition.

And uniformly mixing 0.0801g of glucopyranoside and 0.1g of glycerol to obtain the anti-aging composition.

Comparative example 53: preparation of an anti-aging composition.

And uniformly mixing 0.0801g of glucan and 0.1g of glycerol to obtain the anti-aging composition.

Comparative example 54: preparation of an anti-aging composition.

And uniformly mixing 0.0801g of sodium hyaluronate and 0.1g of glycerol to obtain the anti-aging composition.

Comparative example 55: preparation of an anti-aging composition.

And uniformly mixing 0.0801g of panthenol and 0.1g of glycerol to obtain the anti-aging composition.

Preparing essence:

(1) The anti-aging compositions prepared in examples 2 to 11 and comparative examples 38 to 55 were prepared into essences according to the formulas of table 2, respectively, to obtain the essences of examples 12 to 21 and comparative examples 56 to 73, respectively.

Table 2. Serum formula.

Starting materials	Mass fraction(％)
		Anti-aging composition	25
Chinese gum	0.06
		Acryloyldimethyl ammonium taurate/VP copolymer	0.5
OLIVEM 1000	0.2
		Polydimethylsiloxane	1.5
Hydrolyzed oat protein	1
		Essence	0.02
Deionized water	Allowance of

(2) The preparation method of the essence comprises the following steps:

A. adding deionized water into a stirring pot, adding acryloyl dimethyl ammonium taurate/VP copolymer and xanthan gum into the stirring pot, stirring until the mixture is completely and uniformly dispersed, heating to 70-85 ℃, and homogenizing at high speed for 5min.

B. Adding polydimethylsiloxane and OLIEVM 1000, mixing uniformly, adding the anti-aging compositions of examples 2-11 and comparative examples 38-55 respectively, stirring and heating to 40-55 ℃, dissolving completely, homogenizing at high speed for 5min, cooling to 40 ℃, adding essence and hydrolyzed oat protein, and stirring until the material is uniform to obtain the essence.

And (3) testing the anti-aging performance:

285 healthy skin volunteers aged 30-50 years were selected and randomized into an average of 57 groups of 5 individuals each.

The facial mask liquid is matched with a radio frequency beauty instrument for use: randomly selecting 140 volunteers, cleaning faces, respectively applying the essence liquids of examples 12-21 and comparative examples 56-73 on the faces and the canthus of the volunteers, and dispersing the essence liquids to the faces and the canthus for absorption by using a radio frequency beauty instrument for 20min; used 3 times per week for 4 weeks; the mean values of the respective indices were recorded after 4 weeks of the test.

Use of facial mask solution only: randomly selecting 140 volunteers, cleaning face, respectively applying the essence solutions of examples 12-21 and comparative examples 56-73 on face and canthus of volunteer, and absorbing the essence solution on face and canthus for 20min; used 3 times per week for 4 weeks; the average of the respective indices after 4 weeks of the test was recorded.

Use of radio frequency beauty treatment instrument only: randomly selecting 5 volunteers, cleaning face, and using radio frequency beauty instrument for 20min; used 3 times per week for 4 weeks; the mean values of the respective indices were recorded after 4 weeks of the test. The results are shown in Table 3.

Table 3, results of the anti-aging property test of the essences of examples 12 to 21 and comparative examples 56 to 73.

As can be seen from Table 3, by comparing examples 12-21 with comparative examples 56-73, it can be seen that the combination of the antioxidant, the complex polypeptide, the panthenol and the glycoside compound in a specific ratio has a synergistic effect on wrinkle removal of the essence. Meanwhile, the compounding of the antioxidant, the compound polypeptide, the panthenol and the glycoside compound which are outside the specific proportion does not have obvious synergistic effect on the repair and the anti-aging of the essence. The antioxidant, the composite polypeptide, the panthenol and the glycoside compound in a specific ratio are mixed with other moisturizers in the invention, and the obtained anti-aging composition also has a synergistic wrinkle removing effect consistent with the essence prepared in examples 12-21.

The change of skin wrinkles can be known by comparing the use of the facial mask liquid and the radio frequency cosmetic instrument, only using the facial mask liquid and only using the radio frequency cosmetic instrument: when only the radio frequency beauty instrument is used, the skin wrinkles are slightly reduced, which shows that the radio frequency beauty instrument can reduce the wrinkles; when only facial mask liquid is used, skin wrinkles are slightly reduced; when the facial mask liquid is matched with a radio frequency beauty instrument for use, the skin wrinkle reducing effect is greatly increased, so that the cosmetic prepared from the anti-aging composition can promote the regeneration of dermal collagen of skin and fill and fade fine lines by matching the heat effect of the radio frequency beauty instrument, and achieves the effects of deep anti-aging, long-acting moisture retention, wrinkle resistance and skin nourishing.

And (3) testing the irritation:

200 healthy skin volunteers aged 18-40 years were selected and randomly divided equally into 10 groups of 20 individuals each. By adopting a 48-hour closed spot test method, 0.02mL of the essence products of examples 12-21 are respectively taken and put into a spot tester and applied to the inner side of the forearm of a testee, a radio frequency beauty instrument is used for dispersing the essence on the inner side of the forearm of the testee, the tested object is removed after 24 hours, the spot-pasted part is kept dry, violent movement, scratching of the spot-tested part, long-time sunlight irradiation and the like are avoided, the tester is removed and marked after 48 hours, judgment is carried out under sufficient light after 30 minutes, and return visit observation is carried out after 72 hours respectively.

TABLE 4 cutaneous adverse reaction grading criteria.

And (3) testing results: the results of the irritation test are shown in Table 5.

TABLE 5 results of irritation test for the serums of examples 12-21.

As can be seen from Table 5, the essences of examples 12 to 21 of the present invention are safe and non-irritating.

Finally, it should be noted that the above-mentioned embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the above-mentioned embodiments, it should be understood by those skilled in the art that after reading the present specification, modifications and equivalents of the specific embodiments of the present invention can be made without departing from the scope of the claims of the present application.

Claims

1. An anti-aging composition is characterized by comprising the following raw materials in parts by mass:

2. The anti-aging composition according to claim 1, comprising the following raw materials in parts by mass:

3. The anti-aging composition according to claim 2, comprising the following raw materials in parts by mass:

4. The anti-aging composition as claimed in claim 1, wherein the extraction process of the olive leaf extract comprises the steps of:

5. The anti-aging composition of claim 1, further comprising 1 to 20 parts of a polyhydric humectant having a carbon chain length of C3 to C6.

6. The anti-aging composition of claim 5, further comprising 10 parts of a polyhydric humectant having a carbon chain length of C3 to C6.

7. The anti-aging composition according to claim 6, wherein the polyhydric humectant has a carbon chain length of C3 to C6 and is at least one selected from glycerin, propylene glycol, butylene glycol, methyl propylene glycol, rhamnose, erythritol, arabinose, and saccharide isomers.

8. The anti-aging composition according to claim 7, wherein the polyhydric humectant has a carbon chain length of C3 to C6 and is at least one selected from glycerin and rhamnose.

9. Use of the antiaging composition according to any one of claims 1 to 8 for the preparation of a cosmetic comprising the above antiaging composition, wherein the cosmetic is selected from any one of a facial mask liquid, an emulsion, a cream, a toner, a essence, a stock solution, a face cleanser, a toner, a perfume, a makeup remover, a foundation cream, and a concealer.

10. The anti-aging composition of any one of claims 1 to 8 for use in radio frequency cosmetic and medical cosmetic skin care.