CN115327090A - Platelet detection device - Google Patents

Abstract

The application discloses platelet detection device includes: the turntable mechanism is provided with a carrier assembly in a detachable mode, the carrier assembly comprises a first carrier assembly or a second carrier assembly, and the carrier assembly is used for placing samples or reagent bottles of corresponding types; wherein the first carrier assembly is used for carrying out antibody detection experiments, and the second carrier assembly is used for carrying out cross matching experiments; the centrifugal incubation mechanism is arranged on one side of the turntable mechanism and comprises a centrifugal machine and an incubator, the centrifugal machine is used for centrifuging samples in the microplate holes, the incubator is used for incubating the samples in the microplate holes, and a first window is formed in the upper end of the incubator; the sample adding mechanism comprises a first driving component and a sample adding needle, and the first driving component is used for driving the sample adding needle to add samples into the hole site; and an interpretation mechanism. This application is through setting up detachable carrier assembly, can compatible antibody detection experiment and cross match type experiment effectively to reduce the detection cost of platelet.

Description

Platelet detection device

Technical Field

The application relates to the technical field of medical equipment, in particular to a platelet detection device.

Background

Platelet transfusion is an effective means of preventing and treating bleeding caused by thrombocytopenia or functional defects, and reducing the fatality rate of bleeding caused by thrombocytopenia after radiotherapy and chemotherapy. As the clinical popularization of platelet infusion has increased, the ineffectiveness of platelet infusion has also attracted increasing attention.

At present, two modes of antibody detection and cross matching are generally adopted as platelet detection modes. The platelet antibody detection antigen can assist a doctor to obtain the reason of ineffective platelet infusion by detecting the platelet antibody; the doctor can also be guided to select a proper platelet infusion scheme for the patient so as to reasonably and effectively utilize precious blood resources; and the corresponding antibody in the serum can be regularly measured for patients with repeated platelet infusion to predict the platelet infusion effect and timely discover immune factors causing ineffective platelet infusion. The cross matching can distinguish the types of the platelet antibodies of patients; and the corresponding antigen-negative platelets can be selected for transfusion for the patient so as to improve the transfusion effect of the platelets.

The existing platelet antibody detection experiment and the cross matching experiment need to be respectively carried out on a corresponding antibody detection device and a corresponding cross matching device, and the existing platelet antibody detection experiment and the cross matching experiment need to have both platelet detection and cross matching so as to meet different detection requirements, thereby leading to higher cost of platelet detection.

Disclosure of Invention

In order to solve the problem that the existing platelet detection device cannot be compatible with an antibody detection experiment and a cross matching experiment, the scheme of the application provides the platelet detection device, which can be used for performing the platelet antibody detection experiment and the cross matching experiment of platelets, so that the detection cost of the platelets is reduced.

The platelet testing device according to the embodiment of the application comprises: the rack assembly comprises a first rack assembly or a second rack assembly, and the rack assembly is provided with a test tube hole for placing a reagent bottle loaded with a sample or a corresponding type of reagent; wherein the first carrier assembly is used for carrying out antibody detection experiments, and the second carrier assembly is used for carrying out cross matching experiments; the centrifugal incubation mechanism is arranged on one side of the turntable mechanism and comprises a centrifuge and an incubator, a micro-slab strip is arranged in the incubator, the centrifuge is arranged in the incubator and connected with the micro-slab strip, a plurality of micro-slab holes are formed in the micro-slab strip, the centrifuge is used for centrifuging samples in the micro-slab holes, the incubator is used for incubating the samples in the micro-slab holes, and a first window is formed in the upper end of the incubator; the sample adding mechanism is arranged above the turntable mechanism and comprises a first driving assembly and a sample adding needle, and the first driving assembly is used for driving the sample adding needle to add samples into the hole sites; and the interpretation mechanism is arranged above the turntable mechanism and is used for interpreting the experimental result of the sample.

The platelet testing device according to the embodiment of the first aspect of the application has at least the following beneficial effects: when the antibody detection experiment is carried out, a first carrier assembly can be placed on the turntable mechanism, and samples and reagents required by the antibody detection experiment are respectively placed at different positions of the first carrier assembly so as to carry out the antibody detection experiment; when the cross matching experiment is carried out, the second carrier assembly can be placed on the turntable mechanism, and samples and reagents required by the cross matching experiment are respectively placed at different positions of the second carrier assembly so as to carry out the cross matching experiment. This application is through setting up detachable carrier assembly on platelet detection device, can compatible antibody detection experiment and cross match type experiment effectively to reduce the detection cost of platelet.

According to some embodiments of the application, carousel mechanism is annular hollow structure, and carousel mechanism is provided with first drive unit and second drive unit, and the carrier subassembly sets up on carousel mechanism's outer lane, and first drive unit and second drive unit all are connected with the carrier subassembly, and the incubator sets up in carousel mechanism's middle part, and first drive unit is used for driving carousel mechanism to drive the carrier subassembly rotatory to predetermineeing the position, and the second drive unit is used for driving reagent bottle rotation and shakes evenly. Through setting up carousel mechanism into annular hollow structure, will hatch the ware and arrange the middle part of carousel mechanism in, set up carrier assembly on the outer lane of carousel mechanism, can effectively utilize the space, reduce the occupation space of centrifugal incubation mechanism, when needing the application of sample, first drive unit drives the outer lane rotation of carousel mechanism, will correspond sample position rotation to predetermineeing the position to carry out the processing that corresponds to the sample, second drive unit is used for the drive to load and remains the reagent bottle of mixing reagent and rotate the mixing.

Further, the first carrier assembly includes a reagent carrier and a sample carrier. The reagent carrier and the sample carrier required by antibody detection are placed on the outer ring of the turntable mechanism so as to respectively rotate the sample carrier and the reagent carrier to the lower part of the sample adding mechanism, and the sample and the reagent are sequentially added to the centrifugal incubation mechanism through the sample adding needle to perform the next action.

Further, the first carrier assembly comprises 4 sample carriers and 1 reagent carrier, and by arranging 4 sample carriers and 1 reagent carrier, the detection of a plurality of samples can be completed in each batch, and the detection efficiency is effectively improved.

According to some embodiments of the present application, the second carrier assembly comprises a sample carrier, a reagent carrier and a blending carrier, the blending carrier being connected with the second drive unit. And reagent carriers, sample carriers and blending carriers required by cross matching are placed on the outer ring of the turntable mechanism, the blending carriers are connected with the second driving unit, the second driving unit drives the blending carriers to blend the reagents placed in the blending carriers, and then the blended reagents and samples are moved to the centrifugal incubation mechanism to perform the next action.

Further, the second carrier assembly includes 1 sample carrier, 1 reagent carrier, 1 eight-bit blending carrier, and 2 four-bit blending carriers. Through setting up eight mixing carriers of 1 and 2 quadbit mixing carriers, multiunit reagent of mixing simultaneously improves mixing efficiency to improve the experimental efficiency of cross matching type.

According to some embodiments of the application, the application of sample mechanism is provided with two sets of application of sample channels, and every group application of sample channel is provided with at least one application of sample needle and a drive unit, and two sets of application of sample channels carry out independent elevating movement in vertical direction. Through setting up the application of sample passageway that two sets of independence go up and down, can carry out the application of sample to two sample positions simultaneously to improve application of sample efficiency, improve the antibody detection of platelet and/or cross experimental efficiency who matches the type.

According to some embodiments of the present application, the interpretation mechanism is connected to the sample application mechanism, and the interpretation mechanism includes a fifth driving unit for driving the interpretation mechanism to move in the vertical direction. By connecting the interpretation mechanism with the sample adding mechanism, the interpretation mechanism can be lifted and lowered to interpret, and meanwhile, the occupied space of the interpretation mechanism is saved.

Furthermore, the platelet detection device also comprises a deflector rod, the deflector rod is connected with the fifth driving unit, and the deflector rod is used for opening or closing the first window.

Further, a magnet is arranged at the lower end of the shifting rod, a blocking piece is arranged at the upper end of the first window, and the magnet is used for opening or closing the first window in a matching mode with the blocking piece.

Furthermore, the interpretation mechanism is also provided with an induction component, and the induction component is used for inducing the spatial position of the interpretation mechanism. Through setting up the response subassembly, can avoid interpreting the mechanism and fail to aim at and appear the problem that the rate of accuracy of interpreting is low to interpretation mechanism accurate positioning.

Furthermore, the interpretation mechanism further comprises a camera, a camera box and a shading sleeve, wherein the camera is arranged in the middle of the camera box, and the shading sleeve is arranged at the lower end of the camera box. Set up the shading sleeve at camera box lower extreme, can effectively avoid the interference of ambient light, improve the rate of accuracy of interpreting the experimental result.

According to some embodiments of the present application, the platelet testing device further comprises a plate washing mechanism, wherein the plate washing mechanism comprises a plate washing head assembly and a second driving assembly, and the second driving assembly is used for driving the plate washing head assembly to move up and down. Through setting up the washing trigger subassembly, can in time wash the application of sample needle, so can save the consumptive material cost of cleaner, when saving the cost, can effectively improve the rate of accuracy of platelet detection experiment.

Furthermore, wash the head subassembly and include a set of notes liquid needle and imbibition needle at least, annotate liquid needle and imbibition needle and set up in the lower extreme of washing the head subassembly, annotate liquid needle and/or imbibition needle and be provided with dodge portion, dodge portion to annotating the middle of liquid needle and imbibition needle and buckling. By arranging the liquid injection needles and the liquid suction needles into a group, liquid injection and liquid suction actions can be respectively carried out in the same microplate hole, and the time for washing the microplate is shortened. When the diameter of the microplate hole is smaller, the liquid injection needle and the liquid suction needle are simultaneously inserted into the same microplate hole, so that the phenomenon of needle striking is easy to occur, namely, the liquid injection needle or the liquid suction needle strikes the edge or the inner wall of the microplate hole, and the condition of cleaning failure is caused. Therefore, the avoidance part is arranged at the preset position of the liquid injection needle and/or the liquid suction needle, and the avoidance part is bent towards the middle of the liquid injection needle and the liquid suction needle, so that the risk of firing pin can be effectively avoided.

Furthermore, a maintenance pool is arranged on one side of the second driving assembly and used for cleaning and maintaining the plate washing head assembly. Through setting up the maintenance pond of washing the plate head subassembly of maintenance of washing, can in time wash the maintenance to washing the plate head subassembly, when avoiding cross contamination sample, also can improve the life who washes the plate head subassembly.

Further, wash trigger structure still including washing the first pendant of trigger and washing the first stores pylon of trigger, wash the first end of trigger and still be provided with first fixed orifices and first regulation hole, wash the trigger through first fixed orifices, first regulation hole and wash first pendant fixed connection of trigger, first regulation hole is used for adjusting the angle of washing the trigger at the horizontal direction. Through setting up the first regulation hole of adjusting washing plate head subassembly at the horizontal direction angle, can the accurate position of adjusting washing plate head subassembly in the horizontal direction to realize the accurate positioning of washing plate head subassembly and microplate hole, can avoid the inaccurate and unable problem that inserts the microplate hole that corresponds or appear the firing pin of location.

Additional aspects and advantages of the present application will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the present application.

Drawings

For a clearer explanation of the embodiments of the present application or technical solutions in the prior art, the drawings needed to be used in the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings can be obtained by those skilled in the art according to the drawings.

Fig. 1 is a schematic view of the overall structure of a platelet testing device according to an embodiment of the present application;

fig. 2 is a schematic structural view of a turntable mechanism of the platelet testing device according to the embodiment of the present application;

fig. 3 is a schematic structural view of a turntable mechanism of a platelet testing device according to another embodiment of the present application;

fig. 4 is a partial structural schematic view of a turntable mechanism of the platelet testing device according to the embodiment of the present application;

fig. 5 is a schematic structural diagram of a sample application mechanism and an interpretation mechanism of the platelet testing device according to the embodiment of the present application;

FIG. 6 is an exploded view of the interpretation mechanism of the platelet testing device according to the embodiment of the present application;

FIG. 7 is a schematic structural view of a plate washing mechanism of the platelet testing device according to the embodiment of the present application;

FIG. 8 is a schematic structural view of a plate washing mechanism of a platelet testing device according to another embodiment of the present application;

FIG. 9 is a schematic view of a cartridge assembly of the platelet testing device according to the embodiment of the present application;

FIG. 10 is a cross-sectional view of a wash head assembly of a platelet testing device according to an embodiment of the present application;

fig. 11 is a top view of a wash head assembly of a platelet testing device according to an embodiment of the present application.

Reference numerals:

the mixing device comprises a turntable mechanism 100, a reagent carrier 110, a sample carrier 120, a mixing carrier 130, an eight-bit mixing carrier 131, a four-bit mixing carrier 132, a second driving unit 133, a handheld part 140, a turntable base 150, a carrier base 160, a positioning column 170, a foolproof column 180, a supporting column 190, a centrifugal incubation mechanism 200, an incubator 210, a first window 211, a sample adding mechanism 300, a first sample adding channel 310, a first sample adding needle 311, a third driving unit 312, a second sample adding channel 320, a second sample adding needle 321, a fourth driving unit 322, a first fixing part 323, a second fixing part 324, a first connecting part 325, a first connecting part 326, a second linear guide 327, a fifth driving unit 328, a second connecting part 311, a third driving unit 312, a fourth driving unit 322, a first fixing part 323, a second fixing part the device comprises a first linear guide rail 330, a first screw rod 350, a shift lever 360, a magnet 361, a second connecting piece 362, a limit nail 363, an interpretation mechanism 400, a camera 410, a camera box 420, a shading sleeve 430, a camera hanging piece 440, a cover plate 450, a sensing assembly 460, a positioning sensor 461, a sensing piece 462, a third fixing piece 470, a plate washing mechanism 500, a plate washing head assembly 510, a liquid injection needle 511, a liquid suction needle 512, an avoiding part 513, a first adjusting hole 514, a first fixing hole 515, a second driving assembly 520, a third linear guide rail 530, a second screw rod 540, a hub 550, a maintenance pool 560, a support 570, a plate washing head hanging piece 580, a plate washing head hanging frame 590 and a U-shaped groove 591.

Detailed Description

Reference will now be made in detail to the embodiments of the present application, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present application and are not to be construed as limiting the present application.

In the description of the present application, it should be understood that the positional descriptions, such as the upper, lower, left, right, front, rear, etc., referred to the positional or orientational relationships described based on the drawings are only for convenience of description and simplification of the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the application.

In the description of the present application, the meaning of a plurality is two or more, greater than, less than, etc. are understood to exclude the present number, and at least, not greater than, below, within, etc. are understood to include the present number. The description of first, second, etc. in this application is for the purpose of distinguishing between technical features and is not intended to indicate or imply relative importance or to implicitly indicate the number of technical features indicated or to implicitly indicate the precedence of technical features indicated.

In the description of the present application, unless otherwise specifically limited, terms such as disposed, connected and the like should be understood broadly, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present application in combination with the detailed contents of the technical solutions.

As the clinical popularization of platelet infusion has increased, the ineffectiveness of platelet infusion has also attracted increasing attention. In the related art, the antibody type of platelets is generally determined by two experimental methods, platelet antibody detection and cross-matching, so as to avoid the occurrence of ineffective infusion. The two experiments need to be respectively carried out on a corresponding antibody detection device and a cross matching device, and in order to meet different detection requirements, platelet antibody detection and cross matching need to be carried out simultaneously, so that the detection cost of platelets is obviously increased.

In order to solve the problem that the platelet detection device cannot be compatible with an antibody detection experiment and a cross matching experiment, the scheme of the application provides the platelet detection device, which can be used for performing the platelet antibody detection experiment and the cross matching experiment of platelets, so that the detection cost of the platelets is reduced.

Referring to fig. 1 and 5, the present application proposes a platelet testing device including: a carousel mechanism 100 on which carriage assemblies are detachably disposed, the carriage assemblies including a first carriage assembly or a second carriage assembly, the carriage assemblies being provided with test tube holes for placing reagent bottles loaded with samples or corresponding types of reagents; the first carrier assembly is used for carrying out antibody detection experiments, and the second carrier assembly is used for carrying out cross matching experiments; the centrifugal incubation mechanism 200 is arranged on one side of the turntable mechanism 100, the centrifugal incubation mechanism 200 comprises a centrifuge and an incubator 210, a microplate strip is arranged in the incubator 210, the centrifuge is arranged in the incubator 210 and connected with the microplate strip, a plurality of microplate holes are formed in the microplate strip, the centrifuge is used for centrifuging samples in the microplate holes, the incubator 210 is used for incubating the samples in the microplate holes, and a first window 211 is formed in the upper end of the incubator 210; the sample adding mechanism 300 is arranged above the turntable mechanism 100, the sample adding mechanism 300 comprises a first driving component and a sample adding needle, and the first driving component is used for driving the sample adding needle to add samples into the hole site; the interpretation mechanism 400 is disposed above the turntable mechanism 100, and the interpretation mechanism 400 is used for interpreting the experimental result of the sample.

Specifically, when performing an antibody detection experiment, a first carrier assembly may be placed on the turntable mechanism 100, and samples and reagents required for the antibody detection experiment are placed at different positions of the first carrier assembly, respectively, so as to perform the antibody detection experiment; in performing the cross-fitting experiment, a second carriage assembly may be placed on the turntable mechanism 100, and samples and reagents required for the cross-fitting experiment may be placed at different positions of the second carriage assembly, respectively, so as to perform the cross-fitting experiment. That is, this application scheme is through setting up the first carrier subassembly of detachably and second carrier subassembly, and compatible platelet antibody detects the experiment and alternately matches the type experiment effectively to satisfy different platelet and detect the demand, reduce the detection cost of platelet.

Referring to fig. 2 to 4, in some embodiments of the present application, the carousel mechanism 100 is an annular hollow structure, the carousel mechanism 100 is provided with a first driving unit and a second driving unit 133, the carrier assembly is disposed on an outer ring of the carousel mechanism 100, both the first driving unit and the second driving unit 133 are connected to the carrier assembly, the incubator 210 is disposed in the middle of the carousel mechanism 100, the first driving unit is configured to drive the carousel mechanism 100 to drive the carrier assembly to rotate to a predetermined position, and the second driving unit 133 is configured to drive the reagent bottles to rotate and mix uniformly. Through setting up carousel mechanism 100 into annular hollow structure, will hatch ware 210 and arrange carousel mechanism 100's middle part in, set up the carrier subassembly on carousel mechanism 100's outer lane, can effectively utilize the space, reduce the occupation space that mechanism 200 was hatched in the centrifugation, when the needs application of sample, first drive unit drives carousel mechanism 100's outer lane and rotates, will correspond sample position rotation to presetting the position to carry out the processing that corresponds to the sample. The second driving unit 133 is used for driving the reagent bottle loaded with the reagent to be mixed to rotate and mix.

Specifically, carousel mechanism 100 still is provided with carrier base 160 and carousel base 150, be connected with support column 190 between carousel base 150 and the carrier base 160, carousel base 150 is connected with first drive unit, first drive unit drives carousel base 150 and rotates, and then drives carrier base 160 and carrier component along the center of annular hollow structure rotatory, first drive unit can set up to step motor, the rotation angle of motor is controlled by the system to control the carrier component and rotate to predetermined application of sample position, carry out subsequent application of sample action.

Referring to fig. 2, in some embodiments of the present application, the first carrier assembly includes a reagent carrier 110 and a sample carrier 120. The reagent carrier 110 and the sample carrier 120 required for antibody detection are placed on the outer ring of the turntable mechanism 100, so that the sample carrier 120 and the reagent carrier 110 are respectively rotated to the lower part of the sample adding mechanism 300, and the sample and the reagent are sequentially added to the centrifugal incubation mechanism 200 through the sample adding needle, and then the next action is performed.

Specifically, the first carrier assembly comprises 4 sample carriers 120 and 1 reagent carrier 110, and by arranging 4 sample carriers 120 and 1 reagent carrier 110, a plurality of samples can be placed at one time, so that the action of placing the samples on the outer ring of the turntable mechanism 100 for multiple times is avoided, and the detection of a plurality of samples can be completed in each batch, thereby effectively improving the detection efficiency.

Of course, the number of sample carriers 120 and reagent carriers 110 at this time is related to the size and model of the sample carriers 120 and reagent carriers 110 and the diameter of the outer ring of the carousel mechanism 100, and in order to fully utilize the outer ring of the carousel mechanism 100, the sample carriers 120 and reagent carriers 110 may be distributed over the outer ring of the carousel mechanism 100. Accordingly, other numbers of sample carriers 120 and reagent carriers 110 may be provided in conjunction with the carousel mechanism 100.

Referring to fig. 3, in some embodiments of the present application, the second carrier assembly comprises a sample carrier 120, a reagent carrier 110 and a mixing carrier 130, the mixing carrier 130 being connected with a second drive unit 133. The reagent carriers 110, the sample carriers 120 and the blending carriers 130 required for cross matching are placed on the outer ring of the turntable mechanism 100, the blending carriers 130 are connected with the second driving unit 133, the second driving unit 133 drives the blending carriers 130 to blend the reagents placed in the blending carriers, and then the blended reagents and samples are moved to the centrifugal incubation mechanism 200 for the next operation.

In particular, the second carrier assembly includes 1 sample carrier 120, 1 reagent carrier 110, 1 eight-bit blending carrier 131, and 2 four-bit blending carriers 132. Through setting up 1 eight mixing carrier 131 and 2 quadbit mixing carrier 132, can the multiunit reagent of mixing simultaneously, improve mixing efficiency to improve the experimental efficiency of crossing joining in marriage the type.

Similarly, the specifications and the number of the sample carriers 120, the reagent carriers 110, and the kneading carriers 130 at this time are related to the specifications and the types of the sample carriers 120, the reagent carriers 110, and the kneading carriers 130, and the diameter of the outer ring of the turntable mechanism 100, and the sample carriers 120, the reagent carriers 110, and the kneading carriers 130 can be distributed over the outer ring of the turntable mechanism 100 in order to fully utilize the outer ring of the turntable mechanism 100. Accordingly, other numbers of sample carriers 120, reagent carriers 110, and mixing carriers 130 can be provided in conjunction with the carousel mechanism 100.

Referring to fig. 4, it can be appreciated that to facilitate positioning of the carrier assembly on turntable mechanism 100, positioning posts 170 and fool-proof posts 180 can be provided on carrier base 160, positioning posts 170 being used for positioning of the carrier assembly and fool-proof posts 180 being used for limiting to avoid misplacing the carrier assembly. Of course, to facilitate the pick and place of the carrier assembly, a hand grip 140 may be provided on the sample carrier 120, reagent carrier 110, and mixing carrier 130.

Referring to fig. 5 and 6, in some embodiments of the present application, the sample adding mechanism 300 is provided with two sets of sample adding channels, each set of sample adding channels is provided with at least one sample adding needle and one driving unit, and the two sets of sample adding channels perform independent lifting motion in a vertical direction. Through setting up the application of sample passageway that two sets of independence go up and down, can carry out the application of sample to two sample positions simultaneously to improve application of sample efficiency, improve the antibody detection of platelet and/or cross experimental efficiency who matches the type.

Specifically, the two sets of sample adding channels are a first sample adding channel 310 and a second sample adding channel 320, a first sample adding needle 311 is disposed in the first sample adding channel 310, a second sample adding channel 320 is disposed in the second sample adding needle 321, the first sample adding needle 311 is driven by a third driving unit 312 to correspond to a synchronizing wheel and a synchronizing belt, so as to drive a first lead screw 350, the second sample adding needle 321 is driven by a fourth driving unit 322 to rotate through the corresponding synchronizing wheel and the synchronizing belt, the third driving unit 312 and the fourth driving unit 322 can be motors, of course, a first linear guide rail 330 can be further disposed on the first sample adding channel 310, and a second linear guide rail 327 is disposed on the second sample adding channel 320, so as to respectively limit the first sample adding needle 311 and the second sample adding needle 321 to move in the vertical direction.

It is understood that the loading mechanism 300 can also be provided with three sets of loading channels, or even more, but the structure and principle are similar to those described above, and all are considered to be the same as the essence of the loading mechanism 300 of the two sets of loading channels. And will not be described in detail herein.

Referring to fig. 6, in some embodiments of the present application, the interpretation mechanism 400 is connected to the sample application mechanism, and the interpretation mechanism 400 includes a fifth driving unit 328, and the fifth driving unit 328 is used for driving the interpretation mechanism 400 to move in the vertical direction. By connecting the interpretation mechanism 400 with the sample adding mechanism 300, the interpretation mechanism 400 can be lifted for interpretation, and the occupied space of the interpretation mechanism 400 is saved.

It can be understood, interpretation mechanism 400 includes camera 410 and camera box 420 of protection camera 410, camera box 420 lower extreme is connected with shading sleeve 430, it passes first window 211 and descends to the interior microplate hole upper end of incubator 210 to drive interpretation mechanism 400 at first drive assembly, shading sleeve lower extreme and microplate hole site contact, perhaps with the first preset distance in microplate hole site interval, at this moment, when camera 410 shoots the downthehole experimental result of microplate, the interference of external environment light can almost be completely isolated, thereby effectively improve the accuracy rate of interpretation mechanism 400 to the experimental result interpretation.

Further, the platelet testing device further comprises a shift lever 360, the shift lever 360 is connected to the fifth driving unit 328, and the shift lever 360 is used for opening or closing the first window 211.

Further, a magnet 361 is arranged at the lower end of the shift lever 360, a blocking piece is arranged at the upper end of the first window 211, and the magnet 361 is used for being matched with the blocking piece to open or close the first window 211.

Specifically, the fifth driving unit 328 is fixedly connected to the first fixing member 323, and the first fixing member 323 is fixedly connected to the second fixing member 324. The shifting lever 360 is movably connected with the second fixing piece 324 through a second adjusting hole, the second adjusting hole is a kidney-shaped hole, the limiting nail 363 penetrates through the second adjusting hole formed in the shifting lever 360 and is fixedly connected with the second fixing piece 324, a second connecting piece 362 is arranged on the shifting lever 360 and is connected with the interpretation mechanism 400, the shifting lever 360 moves along with the interpretation mechanism 400 in the vertical direction, and the rising or falling amplitude of the shifting lever 360 is the length of the second adjusting hole.

In addition, a first adapter 326 is arranged at the lower end of the fifth driving unit 328, the first adapter 326 is fixedly connected with the shaft end of the fifth driving unit 328, the first adapter 326 is fixedly connected with the third adapter 325, a third fixing member 470 is fixedly arranged on the third adapter 325, the third fixing member 470 is connected with a camera pendant 440, the lower end of the camera pendant 440 is connected with a cover plate 450, and the cover plate 450 is fixedly connected with the camera 410.

Of course, in order to ensure that the moving position of the interpretation mechanism 400 in the horizontal direction can be precisely aligned with the micro-plate hole at the lower end and the descending distance of the interpretation mechanism 400, the sensing assembly 460 is arranged on the interpretation mechanism 400, which can precisely sense the spatial position of the interpretation mechanism 400 and realize the precise positioning of the descending distance of the interpretation mechanism 400 in the horizontal direction.

Specifically, the sensing assembly 460 includes a vertical sensing unit to accurately sense the position of the interpretation mechanism 400 in the vertical direction, so as to accurately position the interpretation mechanism 400 in the vertical direction, thereby improving the accuracy of interpretation.

Wherein the vertical direction sensing unit includes a positioning sensor 461 and a sensing piece 462, wherein the positioning sensor 461 is configured in a U-shape or a ring shape such that the sensing piece 462 passes through the middle portion of the positioning sensor 461, and the positioning sensor 461 senses the distance by which the interpretation mechanism 400 is raised or lowered by sensing the passing distance of the sensing piece 462.

Referring to fig. 7, in some embodiments of the present application, the platelet testing device further includes a plate washing mechanism 500, wherein the plate washing mechanism 500 includes a plate washing head assembly 510 and a second driving assembly 520, and the second driving assembly 520 is used for driving the plate washing head assembly 510 to move up and down. Through setting up wash plate head subassembly 510, can in time wash on the plate head subassembly 510 annotate liquid needle 511 and imbibition needle 512 and wash, so can save the consumptive material cost of cleaner, when saving the cost, can effectively improve the rate of accuracy of platelet detection experiment.

Referring to fig. 9 and 10, further, the washing plate head assembly 510 at least comprises a set of liquid injection needle 511 and liquid suction needle 512, the liquid injection needle 511 and the liquid suction needle 512 are disposed at the lower end of the washing plate head assembly 510, the liquid injection needle 511 and/or the liquid suction needle 512 are/is provided with an avoiding portion 513, and the avoiding portion 513 is bent towards the middle of the liquid injection needle 511 and the liquid suction needle 512. By arranging the liquid injection needle 511 and the liquid suction needle 512 as a group, liquid injection and liquid suction can be respectively carried out in the same microplate hole, and the time for washing the microplate is shortened. When the diameter of the microplate hole is small, the liquid injection needle 511 and the liquid suction needle 512 are inserted into the same microplate hole at the same time, so that the phenomenon of needle collision is likely to occur, that is, the liquid injection needle 511 or the liquid suction needle 512 collides with the edge or the inner wall of the microplate hole, which causes the condition of cleaning failure. Therefore, according to the scheme, the avoidance part 513 is arranged at the preset position of the liquid injection needle 511 and/or the liquid suction needle 512, and the avoidance part 513 is bent towards the middle of the liquid injection needle 511 and the liquid suction needle 512, so that the risk of firing the needle can be effectively avoided.

Referring to fig. 8, further, a maintenance basin 560 is disposed at one side of the second driving assembly 520, and the maintenance basin 560 is used for performing cleaning maintenance on the plate washing head assembly 510. Through setting up the maintenance pond 560 that washs and maintain plate head subassembly 510, can wash the plate head subassembly 510 in time and maintain, when avoiding cross contamination sample, also can improve the life of plate head subassembly 510.

Referring to fig. 9 and 11, further, the plate washing mechanism 500 further includes a plate washing head hanger 580 and a plate washing head hanger 590, the upper end of the plate washing head assembly 510 is further provided with a first fixing hole 515 and a first adjusting hole 514, the plate washing head assembly 510 is fixedly connected with the plate washing head hanger 560 through the first fixing hole 515 and the first adjusting hole 514, and the first adjusting hole 514 is used for adjusting the angle of the plate washing head assembly 510 in the horizontal direction. Through setting up the first regulation hole 514 of adjusting wash plate head subassembly 510 at the horizontal direction angle, can the accurate position of adjusting wash plate head subassembly 510 at the horizontal direction to realize the accurate positioning of wash plate head subassembly 510 and microplate hole, can avoid the inaccurate and unable problem of inserting corresponding microplate hole or appearing the firing pin of location.

Specifically, the plate washing head hanging member 580 is fixedly connected with the plate washing head assembly 510, a U-shaped groove 591 is arranged on the plate washing head hanging frame 590, and the U-shaped groove 591 is used for limiting the plate washing head hanging member 580 to slide along the vertical direction. The number of the first fixing holes 515 is one, the number of the first adjusting holes 514 is two, three holes are distributed in a triangular shape, and screws penetrate through the first fixing holes 515 of the plate washing head hanger 580 and are fixedly connected with the plate washing head assembly 510, so that the plate washing head hanger 580 and the plate washing head assembly 510 are fixedly connected along the first fixing holes 515; the first adjusting hole 514 is a waist-shaped hole, which can adjust the rotation angle of the plate washing head assembly 510 in the horizontal direction, that is, the first fixing hole 515 is used as a base point, the position of the plate washing head assembly 510 is adjusted by rotation, and after the plate washing head assembly is adjusted in place, the screw passing through the first adjusting hole 514 is locked, so that the positions of the plate washing head hanger 580 and the plate washing head assembly 510 are fixed, and the plate washing head assembly 510 can be positioned more accurately. Through the design of the adjustable waist-shaped hole, the machining precision of other matching parts can be reduced while the accurate positioning is realized, and the purpose of reducing the cost is finally achieved.

Of course, the plate washing mechanism 500 further comprises a bracket 570, and the bracket 570 is used for supporting the plate washing head assembly 510 and the maintenance sink 560.

It can be understood that the second driving assembly 520 further includes a motor, a third linear guide 530, a second lead screw 540 and a collector 550, the motor drives the second lead screw 540 to move in the vertical direction along the third linear guide 530 through a synchronous wheel and a synchronous belt connected with the motor, and the plate washing head hanger 590 is fixedly connected with the second driving assembly 520, so as to drive the plate washing head assembly 510 to move in the vertical direction.

In addition, in order to avoid intertwining among the plurality of wire connecting lines, the wire concentrator 550 is adopted to arrange and collect the plurality of wire connecting lines, so that the service life of the wire connecting lines can be prolonged, and the overall aesthetic feeling of the platelet detection device can be improved.

The following describes the procedure of platelet antibody detection and platelet cross matching experiments, specifically describing the method of using the platelet detection device according to the present invention:

the experimental process of detecting the platelet antibody comprises the following steps:

placing the corresponding sample carriers 120 and reagent carriers 110 on the carousel mechanism 100 and the corresponding microplate strips in the incubator 210; the kit comprises a reagent carrier, a sample carrier, a micro-strip, a reagent carrier and a reagent, wherein the number of the sample carrier and the micro-strip is 4, 8 hole sites are arranged on each sample carrier and each micro-strip, and platelet suspension, indicator red blood cells, negative control, positive control, low-ionic-strength solution and anti-human IgG solution are sequentially placed on the reagent carrier;

placing a plurality of test tubes loaded with different samples into corresponding sample holes of the sample carrier 120, and placing a plurality of reagent bottles loaded with different reagents into corresponding reagent holes of the reagent carrier 110;

the reagent bottles containing the indicating red blood cells and the platelets are connected with the second driving unit 133, and the second driving unit 133 drives the reagent bottles loaded with the platelets and the reagent bottles containing the indicating red blood cells to rotate and uniformly mix the reagent bottles;

the fifth driving unit 328 drives the shift lever 360 to descend, and cooperates with the blocking piece at the upper end of the first window 211, and after the first window 211 is opened, the fifth driving unit 328 ascends and returns to the original position;

adding 50 mu L of platelet suspension into each microplate well of the microplate strip;

shaking the platelet suspension and the coating material in the microplate hole of the microplate strip uniformly by using a centrifugal machine to ensure that the platelet suspension is in full contact with the coating material, and centrifuging the platelet suspension for 5 minutes to ensure that the platelet suspension forms a platelet monolayer at the bottom of the microplate hole of the microplate strip;

washing the microplate holes of the microplate by using the microplate washing head assembly 510, and removing supernatant including unbound platelets;

adding 100 mu L of low ionic strength solution into the microplate hole of each microplate strip;

adding 50 mu L of sample and a yin-yang contrast into the microplate hole of each microplate strip;

shaking the microplate holes of the microplate strips uniformly again by using a centrifugal machine;

the fifth driving unit 328 drives the shift lever 360 to descend, and closes the first window 211 and ascend to return to the original position in cooperation with the stop piece at the upper end of the first window 211;

incubating the microplate and microplate wells using an incubator 210 for 30 minutes at 37 ℃;

the fifth driving unit 328 drives the shift lever 360 to descend, and cooperates with the blocking piece at the upper end of the first window 211, and after the first window 211 is opened, the fifth driving unit 328 ascends and returns to the original position;

washing the microplate holes of the microplate by using the plate washing head assembly 510, and sucking away the cleaning solution and the unbound samples;

adding 50 mu L of anti-human IgG solution into the microplate hole of each microplate strip;

adding 50 μ L of indicator red blood cells to the microplate well of each microplate strip; shaking the micro-slab uniformly by using a centrifugal machine, and centrifuging;

after the centrifugal incubation, the fifth driving unit 328 drives the shift lever 360 again to open the incubator 210, and drives the interpretation mechanism 400 to descend for a preset distance (the light shielding sleeve 430 contacts with the upper end of the microplate well, or is spaced for a preset distance), so as to interpret the experimental result in the microplate well, and obtain the detection result of the platelet antibody.

The experimental process of the platelet cross matching comprises the following steps:

placing the corresponding sample carrier 120, reagent carrier 110, and mixing carrier 130 on the carousel mechanism 100, and placing the corresponding microplate strip in the incubator 210; wherein, an anti-human IgG solution, a positive control, a negative control and a low ionic strength solution are sequentially placed on the reagent carrier; indicating that the red blood cells are placed in the designated hole locations on the blending carrier 130;

placing a plurality of reagent vials loaded with platelet suspensions of different donor platelets on the mixing carriage 130;

respectively mixing the test tube in which the platelet suspension is placed on the mixing carrier 130 and the reagent bottle in which the indicating red blood cells are placed uniformly;

the fifth driving unit 328 drives the shift lever 360 to descend, and cooperates with the blocking piece at the upper end of the first window 211, and after the first window 211 is opened, the fifth driving unit 328 ascends and returns to the original position;

adding 50 μ L of platelet suspension into the microplate well of each microplate strip;

shaking the platelet suspension evenly by using a centrifugal machine to ensure that the platelet suspension is fully contacted with the coating objects in the microplate holes of the microplate strips, and then centrifuging for 5 minutes to ensure that the platelet suspension forms a platelet monolayer at the bottoms of the microplate holes of the microplate strips;

washing the microplate wells of the microplate with a wash plate head assembly 510 to remove supernatant including unbound platelets;

adding 100 mu L of low ionic strength solution into the microplate hole of each microplate strip;

adding 50 mu L of sample and a yin-yang contrast into the microplate hole of each microplate strip;

shaking the solution in the microplate hole of the microplate evenly by using a centrifugal machine to ensure that the sample is fully contacted with the platelet monolayer at the bottom in the microplate hole of the microplate;

the fifth driving unit 328 drives the shift lever 360 to descend, and closes the first window 211 and ascend to return to the original position in cooperation with the stop piece at the upper end of the first window 211;

incubating the solution in the microplate wells of the microplate strips at 37 ℃ for 30 minutes using the incubator 210;

the fifth driving unit 328 drives the shift lever 360 to descend, and the fifth driving unit 328 ascends and returns to the original position after the first window 211 is opened by matching with the blocking piece at the upper end of the first window 211;

washing the microplate wells of the microplate by using a wash plate head assembly 510 to remove unbound samples;

adding 50 mu L of anti-human IgG solution into the microplate hole of each microplate strip;

adding 50 μ L of indicator red blood cells to the microplate well of each microplate strip;

shaking up and centrifuging the solution in the microplate holes of the microplate strips by using a centrifuge;

the fifth driving unit 328 drives the shift lever 360 to open the incubator 210 again, and drives the interpretation mechanism 400 to descend by a preset distance (the light shielding sleeve 430 contacts with the upper end of the microplate well, or is spaced by the preset distance), so as to interpret the experimental result in the microplate well, and obtain the detection result of the platelet antibody.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the gist of the present invention.

In the description of the present specification, reference to the description of "one embodiment," "some embodiments," "an illustrative embodiment," "an example," "a specific example," or "some examples" or the like means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present application. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The applicant asserts that the above-described embodiments represent only the basic principles, main features and advantages of the present application. It will be understood by those skilled in the art that the present application is not limited to the embodiments described above, which are merely illustrative of the principles of the application, and that various changes and modifications can be made by one of ordinary skill in the art without departing from the spirit and scope of the application, which is intended to be covered by the claims.

Although embodiments of the present application have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the application, the scope of which is defined in the claims and their equivalents.

Claims (16)

1. A platelet testing device, comprising:

the rack assembly comprises a first rack assembly or a second rack assembly, and the rack assembly is provided with a test tube hole for placing a reagent bottle loaded with a sample or a corresponding type of reagent; wherein the first carrier assembly is used for carrying out antibody detection experiments and the second carrier assembly is used for carrying out cross-matching experiments;

the centrifugal incubation mechanism is arranged on one side of the turntable mechanism and comprises a centrifuge and an incubator, a microplate strip is arranged in the incubator, the centrifuge is arranged in the incubator and is connected with the microplate strip, a plurality of microplate holes are formed in the microplate strip, the centrifuge is used for centrifuging samples in the microplate holes, the incubator is used for incubating the samples in the microplate holes, and a first window is formed in the upper end of the incubator;

the sample adding mechanism is arranged above the turntable mechanism and comprises a first driving assembly and a sample adding needle, and the first driving assembly is used for driving the sample adding needle to add samples into the hole sites;

and the interpretation mechanism is arranged above the turntable mechanism and is used for interpreting the experimental result of the sample.

2. The platelet testing device according to claim 1, wherein the carousel mechanism is an annular hollow structure, the carousel mechanism is provided with a first driving unit and a second driving unit, the carrier assembly is disposed on an outer ring of the carousel mechanism, the first driving unit and the second driving unit are both connected to the carrier assembly, the incubator is disposed in the middle of the carousel mechanism, the first driving unit is used for driving the carousel mechanism to drive the carrier assembly to rotate to a preset position, and the second driving unit is used for driving the reagent bottle to rotate and shake.

3. The platelet testing device of claim 2, wherein the first carrier assembly includes a reagent carrier and a sample carrier.

4. The platelet testing device of claim 3, wherein the first carrier assembly includes 4 sample carriers and 1 reagent carrier.

5. A platelet testing device according to claim 2 wherein the second carrier assembly includes a sample carrier, a reagent carrier and a mixing carrier, the mixing carrier being coupled to the second drive unit.

6. The platelet testing device of claim 5, wherein the second carrier assembly comprises 1 sample carrier, 1 reagent carrier, 1 eight-bit blending carrier, and 2 four-bit blending carriers.

7. The platelet testing device of claim 1, wherein the sample application mechanism is provided with two sets of sample application channels, each set of sample application channels is provided with at least one sample application needle and one driving unit, and the two sets of sample application channels perform independent lifting movement in a vertical direction.

8. The platelet testing device of claim 1, wherein the interpretation mechanism is connected to the sample application mechanism, and the interpretation mechanism includes a fifth driving unit for driving the interpretation mechanism to move in a vertical direction.

9. The platelet testing device of claim 8, further comprising a lever connected to a fifth drive unit, the lever configured to open or close the first window.

10. The platelet testing device according to claim 9, wherein a magnet is disposed at a lower end of the lever, and a stopper is disposed at an upper end of the first window, and the magnet is configured to open or close the first window in cooperation with the stopper.

11. The platelet testing device of claim 8, wherein the interpretation mechanism further comprises a sensing component, and the sensing component is used for sensing the spatial position of the interpretation mechanism.

12. The platelet testing device of claim 8, wherein the interpretation mechanism further comprises a camera, a camera box, and a light blocking sleeve, the camera being disposed in the middle of the camera box, the light blocking sleeve being disposed at a lower end of the camera box.

13. The platelet testing device of claim 1, further comprising a plate washing mechanism, wherein the plate washing mechanism comprises a plate washing head assembly and a second driving assembly, and the second driving assembly is used for driving the plate washing head assembly to move up and down.

14. The platelet testing device according to claim 13, wherein the wash plate head assembly comprises at least one set of a liquid injection needle and a liquid suction needle, the liquid injection needle and the liquid suction needle are disposed at a lower end of the wash plate head assembly, and the liquid injection needle and/or the liquid suction needle are/is provided with an avoiding portion which is bent towards the middle of the liquid injection needle and the liquid suction needle.

15. The platelet testing device of claim 13, wherein a maintenance pool is further disposed on one side of the second driving assembly, and the maintenance pool is used for cleaning and maintaining the washing head assembly.

16. The platelet testing device according to claim 13, wherein the plate washing mechanism further includes a plate washing head hanger and a plate washing head hanger, a first fixing hole and a first adjusting hole are further disposed at an upper end of the plate washing head assembly, the plate washing head assembly is fixedly connected to the plate washing head hanger through the first fixing hole and the first adjusting hole, and the first adjusting hole is used for adjusting an angle of the plate washing head assembly in a horizontal direction.

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