CN115283035A - Sampling liquid dropping method of handheld automatic liquid preparation gun - Google Patents
Sampling liquid dropping method of handheld automatic liquid preparation gun Download PDFInfo
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- CN115283035A CN115283035A CN202211043248.7A CN202211043248A CN115283035A CN 115283035 A CN115283035 A CN 115283035A CN 202211043248 A CN202211043248 A CN 202211043248A CN 115283035 A CN115283035 A CN 115283035A
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- rod
- liquid preparation
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- 239000007788 liquid Substances 0.000 title claims abstract description 224
- 238000002360 preparation method Methods 0.000 title claims abstract description 89
- 238000000034 method Methods 0.000 title claims abstract description 30
- 238000005070 sampling Methods 0.000 title claims abstract description 24
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 claims abstract description 81
- 238000012546 transfer Methods 0.000 claims abstract description 54
- 238000010521 absorption reaction Methods 0.000 claims abstract description 7
- 239000002480 mineral oil Substances 0.000 claims abstract description 7
- 235000010446 mineral oil Nutrition 0.000 claims abstract description 7
- 238000003825 pressing Methods 0.000 claims description 29
- 230000007246 mechanism Effects 0.000 description 8
- 230000008569 process Effects 0.000 description 8
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 6
- 235000017491 Bambusa tulda Nutrition 0.000 description 6
- 241001330002 Bambuseae Species 0.000 description 6
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 6
- 239000011425 bamboo Substances 0.000 description 6
- 230000003044 adaptive effect Effects 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- FGRBYDKOBBBPOI-UHFFFAOYSA-N 10,10-dioxo-2-[4-(N-phenylanilino)phenyl]thioxanthen-9-one Chemical compound O=C1c2ccccc2S(=O)(=O)c2ccc(cc12)-c1ccc(cc1)N(c1ccccc1)c1ccccc1 FGRBYDKOBBBPOI-UHFFFAOYSA-N 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000011900 installation process Methods 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0241—Drop counters; Drop formers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0275—Interchangeable or disposable dispensing tips
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a sampling dropping method of a handheld automatic liquid preparation gun, wherein a piston cylinder of the handheld automatic liquid preparation gun is butted with a liquid-transfering gun head so that the liquid-transfering gun head is firmly sleeved on the piston cylinder; secondly, pouring the biological solution into a solution transfer groove, inserting a pipette head into the solution transfer groove, and pulling a lead rod on the handheld automatic solution preparation gun to move in a gun rod cavity so that the pipette head absorbs solution with the volume of xul; the method comprises the following steps of opening a culture dish from the second time, aligning a handheld automatic liquid preparation gun to the center of the culture dish, then pushing a lead rod on the handheld automatic liquid preparation gun to move towards the direction close to a liquid transfer gun head to perform liquid dropping, then adding mineral oil into the culture dish, and then putting the culture dish into a culture box to complete liquid preparation operation, wherein the handheld automatic liquid preparation gun is used for dripping one or more culture dishes in a liquid absorption mode; and finally, after the solution is instilled, separating the pipette tip from the piston cylinder through a withdrawal sleeve. The invention has convenient operation, better accords with the operation habit of operators and greatly reduces the use cost and the learning cost.
Description
Technical Field
The invention belongs to the technical field of biomedical sampling preparation, and particularly relates to a sampling liquid dropping method of a handheld automatic liquid preparation gun.
Background
At present, the solution preparation in hospitals is finished manually, and a large amount of solution preparation operation occupies most of the time of operators. The process of preparing the liquid generally requires two doctors to cooperate, and the manual operation of long-time mechanical repetition is very labor-consuming. And in the process of continuously preparing a plurality of droplets of biological solution in one culture dish, the following problems are encountered: because the area of the operation surface at the bottom of the culture dish is limited, the sizes of continuously and quickly prepared biological solution droplets are uneven due to different working experiences and proficiency of operators, the cultured droplets are too small to be beneficial to biological material culture, and too large to be easily contacted with walls to collapse or to be fused with adjacent droplets. Meanwhile, the commercial liquid preparation devices in the current market are few and are complex and large-scale automatic devices. The liquid preparation process needs to maintain a sterile environment, the whole process is finished in a super clean bench, and large-scale equipment is not dominant in space utilization and is not flexible enough. The large-scale automatic liquid preparation device is high in selling price and needs regular training and maintenance.
Patent publication No. CN112175825A discloses an automatic liquid preparation instrument. The liquid preparation instrument comprises a liquid preparation mechanism, a transfer mechanism and an incubator, wherein the liquid preparation mechanism is used for completing liquid preparation operation to obtain a finished product, and the transfer mechanism is used for placing the finished product into the incubator for pretreatment. The liquid preparation mechanism automatically finishes the liquid preparation action, so that the position, the size and the shape of the titrated liquid drop are regular, the titration amount of the liquid can be accurately controlled, and the oil covering amount of the liquid can also be accurately controlled; in addition, after the liquid preparation action is finished, the titrated liquid can be immediately placed into an incubator by a transfer mechanism for pretreatment of temperature and PH value adjustment; finally, the whole liquid preparation process does not need manual intervention, thereby greatly reducing the labor cost. However, the liquid preparation mechanism and the transfer mechanism of the device are responsible, and the device occupies a large space, and cannot be applied to a scene of miniaturized sampling.
Disclosure of Invention
The invention aims to provide a liquid dropping sampling method of a handheld automatic liquid preparation gun, which is characterized in that one or more liquid-transfering gun heads are arranged on a corresponding piston cylinder through a pushing gun rod, a lead rod arranged in the pushing gun rod, a connecting cover, a lead cylinder, a lead component arranged in the lead cylinder, a front end cover, a piston cylinder arranged on the front end cover, a gun withdrawing sleeve and liquid-transfering gun heads which are sequentially arranged, a piston rod on the lead component is pushed by the lead rod to move in the piston cylinder, so that the solution is sucked, and the liquid-transfering gun heads can be pushed away from the piston cylinder through the gun withdrawing sleeve, so that the problems of a large-scale automatic liquid preparation device are solved.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a sampling liquid dropping method of a handheld automatic liquid preparation gun comprises a pushing gun rod, a lead rod, a connecting cover, a lead cylinder, a lead component, a front end cover and a liquid-transfering gun head, wherein a gun rod cavity is axially arranged on the pushing gun rod, the lead rod is arranged in the gun rod cavity, one side of the connecting cover is connected with one end of the pushing gun rod, the lead cylinder is provided with a lead cavity, one end of the lead cylinder is connected with the other side of the connecting cover, one end of the lead rod penetrates through the connecting cover and extends into the lead cavity, the lead component is arranged in the lead cavity of the lead cylinder and comprises a plurality of piston rods, the piston rods are arranged at one ends of the lead rods, which are deviated from the lead cylinder, so that the lead rods push the piston rods to move in the lead cavity, one end of the front end cover is connected with one end, which is far away from the connecting cover, and a plurality of piston cylinders matched with the piston rods are arranged on the front end cover, so that the piston rods can be adaptively inserted into the corresponding piston cylinders;
the sampling dropping method comprises the following steps:
s1, mounting a pipette tip: butting a piston cylinder of a handheld automatic liquid preparation gun with a liquid-transfer gun head, wherein the liquid-transfer gun head is connected with one end of the piston cylinder, which is far away from the front end cover, so that the liquid-transfer gun head is firmly sleeved on the piston cylinder, and the liquid-suction range of the liquid preparation gun is adjusted according to requirements;
s2, solution sampling: pouring biological solution into the liquid transfer groove, inserting the gun head into the liquid transfer groove, pulling a lead rod on the handheld automatic liquid preparation gun to move in a cavity of the gun rod along the axial direction of the gun rod, and driving a piston rod in a lead assembly to move in a direction far away from the liquid transfer gun head in the piston cylinder when the lead rod moves so that the liquid transfer gun head absorbs solution with the volume of xul;
s3, liquid dropping: opening a cover of the culture dish, aligning the handheld automatic liquid preparation gun to the center of the culture dish, then pushing a lead rod on the handheld automatic liquid preparation gun to move towards the direction close to the pipette head for liquid dropping, driving a piston rod in a lead assembly to move when the lead rod moves so as to enable the solution in the pipette head to drop into the culture dish, adding mineral oil into the culture dish and then putting the culture dish into a culture box to complete liquid preparation operation, wherein the handheld automatic liquid preparation gun is used for dropping one or more culture dishes by liquid absorption at one time;
s4, separating the pipette tip: and after the solution instillation is finished, the pipette tip is separated from the piston cylinder.
Preferably, the hand-held automatic liquid preparation gun is provided with a liquid transfer button and a liquid amount adjusting gear at one end of the pushing gun rod, which is far away from the connecting cover, one end of the liquid transfer button extends into the gun rod cavity of the pushing gun rod and is connected with the lead rod, and the lead rod is pushed by the liquid transfer button to complete liquid dropping; liquid measure adjusting gear with move liquid button and connect, set up a plurality of gears on the liquid measure adjusting gear, through rotatory liquid measure adjusting gear adjusts the dropping liquid volume at every turn, liquid measure adjusting gear is used for adjusting the produced orientation of pressing at every turn and moves the distance of removal in the barral of a gun cavity, and then adjusts move liquid button and promote the distance that the helical pitch pole removed.
Preferably, the step S3 of pushing the lead rod on the handheld automatic liquid preparation gun to drip includes the following steps: the liquid quantity is adjusted according to the volume requirement of actually required liquid drops, the gear of the liquid quantity adjusting gear is adjusted, then the liquid transfer button is pressed to push the lead rod to enable the solution to be dripped into a vessel, the volume of the liquid drops discharged each time is determined by the gear of the liquid quantity adjusting gear, the solution with the volume of xul is discharged for one time or multiple times, then the solution is added with mineral oil and then is placed into an incubator to complete liquid preparation operation, and therefore the handheld automatic liquid preparation gun can be used for one-time liquid suction and dripping one or more culture vessels.
Preferably, the handheld automatic liquid preparation gun further comprises a gun withdrawing sleeve, one end of the gun withdrawing sleeve is connected with the front end cover or the lead cylinder, and a through hole for the piston cylinder to pass through is formed in the other end of the gun withdrawing sleeve, so that one end, far away from the front end cover, of the piston cylinder extends out of the through hole and is connected with the liquid transfer gun head. By the arrangement, the cylinder body of the piston cylinder can be arranged in the cavity of the gun case, so that the gun case is ensured to be moved back to protect the piston cylinder and prevent the piston cylinder from being damaged after being impacted.
Preferably, the step S4 of detaching the pipette tip comprises the steps of: after the solution instillation is completed, one or more pipette tips are ejected simultaneously by sliding the withdrawal sleeve.
Preferably, the gun withdrawing sleeve is movably connected with the front end cover or the lead cylinder, and the gun withdrawing sleeve can reciprocate relative to the front end cover or the lead cylinder, so that one end, provided with the through hole, of the gun withdrawing sleeve pushes the pipette head to separate the pipette head from the piston cylinder.
Preferably, the gun withdrawing sleeve is connected with the front end cover through an elastic assembly, and the gun withdrawing sleeve is pushed to compress or stretch an elastic piece in the elastic assembly so as to move relative to the front end cover and separate the pipette head from the piston barrel.
Preferably, the elastic component comprises a spring element, a spring pressing block and a fastening bolt, the spring pressing block is fixed at the end part of the gun withdrawing sleeve through the fastening bolt, a mounting hole for assembling the spring element is formed in the front end cover, one end of the spring element is fixed in the mounting hole, and the other end of the spring element is connected with the spring pressing block.
Preferably, the lead assembly further comprises a lead plate, a groove for fixing the piston rod is arranged at one end of the lead plate, which is away from the connecting cover, and one end of the lead plate, which faces the connecting cover, is connected with the lead rod.
Preferably, one end of the lead rod, which is far away from the pushing gun rod, is provided with a lead rod fixing part, and the lead rod fixing part is connected with one side, which faces the connecting cover, of the lead component. Specifically, the lead rod fixing part is fixedly connected with the lead plate, so that the lead rod can push the lead plate to move, and then the piston rod on the lead plate is driven to push the piston cylinder, and therefore pressure difference is caused to achieve a liquid suction function.
Preferably, the lead bar orientation the one end of propelling movement barral of a gun is provided with the sliding block, the confession has been seted up to the lateral wall of propelling movement barral of a gun the side fluting that the sliding block stretches out, through promoting the sliding block is so that the lead bar is in remove in the barral of a gun cavity.
Preferably, the structure of sliding block includes sliding block body, fixture block and promotes the piece, it all sets up on one side lateral wall of sliding block to promote piece and fixture block, it certainly to promote the piece the slope extends the setting on the lateral wall of sliding block body, and promotes the piece and follow stretch out the setting in the side fluting, sets up like this and can be convenient for promote with the hand in the use promote the piece, the fixture block be used for with the joint groove cooperation of propelling movement barral of gun lateral wall, when promoting and promote the piece, it drives to promote the piece sliding block body, fixture block move together, the fixture block is in move in the joint groove, and when the fixture block removed the diapire contact with the joint groove, the joint groove can carry on spacingly to the fixture block to realize spacing sliding block and helical pitch pole, prevent that the helical pitch pole from roll-off in the barral cavity of gun.
Preferably, one side of the connecting cover is provided with a clamping piece connected with the pushing gun rod, and one end of the pushing gun rod connected with the connecting cover is provided with an annular clamping groove matched with the clamping piece.
Has the advantages that:
according to the handheld automatic liquid preparation gun, one or more liquid transfer gun heads are arranged on the corresponding piston cylinder through the pushing gun rod, the lead rod arranged in the pushing gun rod, the connecting cover, the lead cylinder, the lead component arranged in the lead cylinder, the front end cover, the piston cylinder arranged on the front end cover, the gun withdrawing sleeve and the liquid transfer gun head which are sequentially arranged, when the biological solution needs to be sucked, the biological solution is poured into the liquid transfer groove, the gun head is inserted into the liquid transfer groove to pull the sliding block, so that the piston rod on the lead rod pushing lead component moves in the direction far away from the gun withdrawing sleeve in the piston cylinder, the solution is sucked, then the culture dish is opened, the handheld automatic liquid preparation gun is aligned to the center of the culture dish, the sliding block is pushed, the biological solution is dripped into the culture dish, the problem that a plurality of biological solution micro-droplets are continuously prepared in the culture dish, the prepared biological solution micro-droplets are uniform in volume and orderly arranged is solved, time and labor are not wasted in the whole operation process, and the efficiency is obviously improved.
According to the handheld automatic liquid preparation gun, the liquid quantity adjusting gear is provided with a plurality of gears, the liquid quantity adjusting gear is rotated to adjust the liquid quantity at each time, the liquid quantity adjusting gear can adjust the moving distance of the liquid transfer button towards the gun rod cavity, which is generated by pressing the liquid transfer button at each time, so that the moving distance of the liquid transfer button pushing the lead rod 2 is adjusted, the liquid drop volume is accurately controlled, and the xul volume solution sucked in the liquid transfer gun head can be discharged once or many times through the liquid quantity adjusting gear.
A gun withdrawal sleeve in the liquid preparation gun is connected with a front end cover through an elastic assembly, the gun withdrawal sleeve is pushed to compress or stretch an elastic piece in the elastic assembly so as to move relative to the front end cover, the liquid transfer gun heads are separated from the piston barrel, and simultaneous ejection of a plurality of groups of liquid transfer gun heads is sequentially realized. And the elastic piece recovers deformation to realize automatic resilience of the retreat gun sleeve, so that the retreat gun sleeve can be quickly reset.
The handheld automatic liquid preparation gun is simple to process and manufacture, small in size, more flexible in application scene, more in accordance with the operation habit of operators in operation and greatly reduced in cost.
Drawings
FIG. 1 is a front view of a hand-held automatic liquid preparation gun according to embodiment 1;
FIG. 2 is a perspective view of the hand-held automatic liquid preparation gun according to embodiment 1;
FIG. 3 is an exploded perspective view of the hand-held automatic liquid gun according to example 1;
FIG. 4 is an enlarged view at A in FIG. 2;
fig. 5 is an assembly view of the slide block, the lead rod, and the lead assembly of the hand-held automatic liquid preparation gun in embodiment 1;
fig. 6 is a schematic assembly view of a retreat gun jacket and a front end cover of the hand-held automatic liquid preparation gun according to embodiment 1;
FIG. 7 is a schematic view of a lead assembly of the hand-held automatic liquid gun according to example 1;
fig. 8 is an assembly view of the piston cylinder and the front end cap of the hand-held automatic liquid preparation gun in example 5.
Reference numerals
1. Pushing the gun rod; 11. side grooving; 111. a slider body; 112. a pushing block; 113. a clamping block; 12. an annular neck; 121. a notch section; 2. a lead rod; 21. a lead rod fixing member; 3. a connecting cover; 31. a clamping piece; 4. a lead cylinder; 41. a chute; 5. withdrawing the gun sleeve; 51. a lug structure; 52. an elastic member; 521. a spring member; 522. a spring compression block; 523. fastening the bolt; 6. a lead assembly; 61. a piston rod; 62. a lead plate; 7. a front end cap; 8. a piston cylinder pressure plate; 9. a piston cylinder; 91. a snap ring; 10. pipetting gun heads; 13. a pipetting button;
Detailed Description
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following description will be made with reference to the accompanying drawings. It is obvious that the drawings in the following description are only some examples of the invention, and that for a person skilled in the art, other drawings and embodiments can be derived from them without inventive effort.
The technical solution of the present invention is described in detail with specific examples below.
Example 1
As shown in fig. 1-3, a handheld automatic liquid preparation gun comprises a pushing gun rod 1, a lead rod 2 arranged in the pushing gun rod 1, a connecting cover 3, a lead cylinder 4, a lead assembly 6 arranged in the lead cylinder 4, a front end cover 7, a piston cylinder 9 arranged on the front end cover 7, a gun withdrawing sleeve 5 and a pipette head 10, which are arranged in sequence.
A gun rod cavity is axially arranged on the pushing gun rod 1; the lead rod 2 is arranged in the gun rod cavity and moves in the gun rod cavity along the axial direction of the pushing gun rod 1; one side of the connecting cover 3 is connected with one end of the pushing gun rod 1; the connecting cover 3 is connected with the lead cylinder 4 through a screw, the screw can be preferably arranged in a mounting hole on the outer peripheral wall of the lead cylinder 4, and one end of the lead cylinder 2 penetrates through the connecting cover 3 and extends into the lead cavity; the lead assembly 6 is arranged in a lead cavity of the lead cylinder 4, the lead assembly 6 comprises a plurality of piston rods 61, and the piston rods 61 are arranged at one end of the lead rod 2, which is far away from the lead cylinder 4, so that the lead rod 2 pushes the piston rods 61 to move in the lead cavity; one end of the front end cover 7 is connected with one end of the lead cylinder 4 far away from the connecting cover 3, and the front end cover 7 is provided with a plurality of piston cylinders 9 matched with the piston rods 61, so that the piston rods 61 can be inserted into the corresponding piston cylinders 9 in a matching manner; the pipette tip 10 is used for being connected with one end of the piston cylinder 9 far away from the front end cover 7.
Connect and set up on the lid 3 and supply the through-hole that one end of lead pole 2 passed, the one end of lead pole 2 is followed through-hole on the connection lid 3 passes stretch into behind the connection lid 3 in the lead section of thick bamboo 4 with lead subassembly 6 is connected.
The front end cover 7 and the lead cylinder 4 are designed in a split mode, are of independent structures and are fixedly connected through screws.
As shown in fig. 3 and 5, a sliding block is disposed at one end of the lead rod 2 facing the gun rod pushing body 1, a side groove 11 for the sliding block to extend out is disposed on a side wall of the gun rod pushing body 1, and the sliding block is pushed to move the lead rod 2 in the gun rod cavity. The slider is the pipetting button 13 of the liquid preparation gun.
The structure of sliding block includes sliding block body 111, fixture block 113 and promotes piece 112, it all sets up on one side lateral wall of sliding block to promote piece 112 and fixture block 113, it certainly to promote piece 112 slide up the extension setting on sliding block body 111's the lateral wall, and promote piece 112 and follow stretch out the setting in the side fluting 11, sets up like this and can be convenient for promote with the hand in the use promote piece 112, fixture block 113 be used for with the joint groove cooperation of propelling movement barral of gun 1 lateral wall, when promoting and promote piece 112, it drives to promote piece 112 slide block body 111, fixture block 113 move together, fixture block 113 is in move in the joint groove, and when fixture block 113 moved the diapire contact with the joint groove, the joint groove can carry on spacingly to fixture block 113 to realize spacing to sliding block and helical pitch pole 2, prevent that helical pitch pole 2 from the slide-out in the barral cavity.
As shown in fig. 4, one side of the connection cover 3 is provided with a clamping piece 31 connected with the pushing gun rod 1, and one end of the pushing gun rod 1 connected with the connection cover 3 is provided with a ring-shaped clamping groove 12 matched with the clamping piece 31. Joint spare 31 is a ring spare structure, one side of ring groove 12 is provided with gap section 121, sets up like this and is convenient for joint spare 31 on connecting the lid 3 can be followed gap section 121 card is gone into among the ring groove 12 to the completion connect lid 3 with the assembly of promotion barral of a gun.
As shown in fig. 6, the lead assembly 6 further includes a lead plate 62, a groove for fixing the piston rod 61 is provided at an end of the lead plate 62 facing away from the connecting cover 3, and an end of the lead plate 62 facing toward the connecting cover 3 is connected to the lead rod 2.
The lead plate 62 deviates from the groove formed by arranging a plurality of arrays is formed in one end of the connecting cover 3, the clamping tables used for clamping the end part of the piston rod 61 are arranged in the groove, and the end part of the piston rod 61 is clamped and fixed in the groove. The one end that lead rod 2 deviates from propelling movement rifle pole 1 is provided with lead rod mounting 21, lead rod mounting 21 with lead subassembly 6 is towards one side of connecting lid 3 is connected. Specifically, the lead rod fixing member 21 is fixedly connected to the lead plate 62 toward one side of the connecting cover 3, so that the lead rod 2 can push the lead plate 62 to move, and further drive the piston rod 61 on the lead plate 62 to push in the piston cylinder 9, thereby causing a pressure difference to realize a liquid suction function.
The inside sliding groove that is provided with and leads 62 assorted of a section of thick bamboo 4 of said lead, the sliding groove enables the inside slip of only one degree of freedom of lead 62 at a section of thick bamboo 4 of lead to prevent that lead 62 from taking place to rotate at the slip in-process.
One end of the gun withdrawing sleeve 5 is connected with the front end cover 7 or the lead cylinder 4, and the other end of the gun withdrawing sleeve 5 is provided with a through hole for the piston cylinder 9 to pass through, so that one end of the piston cylinder 9, which is far away from the front end cover 7, extends out of the through hole to be connected with the pipette tip 10. By the arrangement, the cylinder body of the piston cylinder 9 can be arranged in the cavity of the gun withdrawing sleeve 5, so that the gun withdrawing sleeve 5 is ensured to protect the piston cylinder 9, and the piston cylinder 9 is prevented from being damaged after being impacted.
The gun withdrawing sleeve 5 is movably connected with the front end cover 7 or the lead cylinder 4, and the gun withdrawing sleeve 5 can reciprocate relative to the front end cover 7 or the lead cylinder 4, so that one end, provided with the through hole, of the gun withdrawing sleeve 5 pushes the pipette head 10 to separate the pipette head 10 from the piston cylinder 9. By means of the arrangement, when the pipette tip 10 needs to be detached from the piston cylinder 9, the end face of the withdrawal sleeve 5 can be in contact with the end face of the pipette tip 10 by only pushing the withdrawal sleeve 5, so that the withdrawal sleeve 5 pushes the pipette tip 10 to be separated from the piston cylinder 9, the pipette tip 10 does not need to be pulled out from the piston cylinder 9 one by one, and the detaching speed of the pipette tip 10 is improved.
The gun withdrawing sleeve 5 is connected with the front end cover 7 through an elastic assembly 52, and the elastic member in the elastic assembly 52 is compressed or stretched by pushing the gun withdrawing sleeve 5, so that the gun withdrawing sleeve 5 moves relative to the front end cover 7, and the pipette tip 10 is separated from the piston barrel 9. The elastic member in the elastic assembly 52 may be a rubber column, a spring member 521, or other elastic member, and can realize automatic resilience of the gun case 5 by restoring deformation. Set up like this, can make quick reset, it can realize popping out simultaneously of multiunit rifle head.
The gun withdrawing sleeve 5 can be connected with one end, facing the lead cylinder 4, of the front end cover 7, an elastic piece in the elastic assembly 52 is arranged between one end, connected with the front end cover 7, of the gun withdrawing sleeve 5 and one end, facing the lead cylinder 4, of the front end cover 7, when the gun withdrawing sleeve 5 is pushed, the gun withdrawing sleeve 5 compresses the elastic piece, so that the gun withdrawing sleeve 5 moves towards the pipette tip 10 and pushes the pipette tip 10 away from the piston cylinder 9, after the pipette tip 10 is separated from the piston cylinder 9, only thrust applied to the gun withdrawing sleeve 5 needs to be cancelled, the elastic piece restores deformation, and the gun withdrawing sleeve 5 is reset through the thrust of the elastic piece.
As shown in fig. 6, in the preferred embodiment, the elastic component 52 includes a spring element 521, a spring pressing block 522, and a fastening bolt 523, the spring pressing block 522 is fixed at the end of the gun sleeve 5 through the fastening bolt 523, the front end cover 7 is provided with a mounting hole for assembling the spring element 521, one end of the spring element 521 is fixed in the mounting hole, and the other end of the spring element 521 is connected to the spring pressing block 522. The arrangement is such that the spring member 521 connects the ejection sleeve 5 with the connection cap 3, and the spring pressing block 522 on the ejection sleeve 5 can compress or stretch the spring member 521 by pushing the ejection sleeve 5.
The gun withdrawing sleeve 5 is provided with a plurality of lug structures 51 at one end connected with the connecting cover 3, the lug structures 51 are provided with mounting grooves for mounting spring pressing blocks 522, fastening bolts 523 transversely penetrate through the lugs and the spring pressing blocks 522 so that the spring pressing blocks 522 are fixed on the gun withdrawing sleeve 5, and one side of the spring pressing blocks 522, facing the connecting cover 3, is also provided with guide posts which are inserted into inner rings of the spring part 521, so that the spring part 521 can be prevented from transversely shaking in the processes of compression, stretching or deformation recovery, and the spring part 521 can not transversely swing and deform in the pushing process of the gun withdrawing sleeve 5.
Spring compact heap 522 with sliding connection between the lateral wall of a helical pitch section of thick bamboo 4, be provided with spout 41 on the lateral wall of a helical pitch section of thick bamboo 4, spring compact heap 522 orientation one side of the lateral wall of a helical pitch section of thick bamboo 4 with spout 41 cooperation, so that move back gun case 5 when removing spring compact heap 522 can the direction is slided in the spout 41.
As shown in fig. 3, the side of the spring pressing block 522 facing the outer side wall of the lead cylinder 4 is an arc-shaped side wall, and the sliding groove 41 is an adaptive arc-shaped groove, so that the smoothness of sliding is facilitated. And in the moving process of the gun case 5, the spring pressing block 522 can slide in the sliding groove 41 on the lead cylinder 4, so that the gun case 5 is guided.
The piston cylinder 9 can be inserted into the pipette tip 10, or a connecting pipe inserted into the piston cylinder 9 is arranged at the end of the pipette tip 10 used for being connected with the piston cylinder 9, the outer diameter of the end of the piston cylinder 9 connected with the pipette tip 10 is smaller than the diameter of the perforation on the withdrawal sleeve 5, and the outer diameter of the end of the pipette tip 10 used for being connected with the piston cylinder 9 is larger than the diameter of the perforation, so that the piston cylinder 9 can extend out from the perforation on the withdrawal sleeve 5, and the withdrawal sleeve 5 can push the pipette tip 10 to separate from the piston cylinder 9 when the withdrawal sleeve 5 moves towards the pipette tip 10. The pipette tip 10 is of a conical structure, and one end of the pipette tip 10 with the largest outer diameter is connected with the piston cylinder 9.
One end of the piston cylinder 9, which is used for being connected with the pipetting gun head 10, is provided with a stepped adaptive structure, the stepped adaptive structure can be in butt joint with the pipetting gun head 10 and realizes sealing, and the stepped adaptive structure is an annular stepped surface arranged on the outer wall of the piston cylinder 9.
The installation process of the handheld automatic liquid preparation gun of the embodiment is as follows:
firstly, one end of the lead rod 2 is connected with the sliding block body 111, the fixture block 113 and the pushing block 112 on the sliding block body 111 extend out of the side open groove 11 of the push gun rod 1, the other end of the lead rod 2 penetrates through the connecting cover 3 to be connected with the lead rod fixing member 21, then the push gun rod 1 is connected with the connecting cover 3 in a clamping mode, after the lead rod fixing member 21 is fixedly connected with the lead plate 62, one end of the lead cylinder 4 is fixedly connected with the connecting cover 3, so that the lead rod 2 and the piston rod 61 on the lead plate 62 are placed in the lead cylinder 4, then the piston cylinder 9 is installed on the front end cover 7, then the front end cover 7 is fixedly connected with the other end of the lead cylinder 4, so that the piston rod 61 and the piston cylinder 9 are arranged in one-to-one correspondence, the piston rod 61 can extend into the piston cylinder 9, after the front end cover 7 and the lead cylinder 4 are installed, the gun head withdrawing sleeve 5 is connected with the lead cylinder 4 through the elastic assembly 52, and finally the liquid transfer gun head 10 is installed on one end of the gun sleeve 5, and the whole gun head is automatically installed in a hand.
The sampling and dripping method of the handheld automatic liquid preparation gun comprises the following steps:
s1, mounting a pipette tip 10: butting a piston cylinder 9 of a handheld automatic liquid preparation gun with a liquid transfer gun head 10, wherein the liquid transfer gun head 10 is connected with one end, far away from the front end cover 7, of the piston cylinder 9, so that the liquid transfer gun head 10 is firmly sleeved on the piston cylinder 9, and the liquid absorption range of the liquid preparation gun is adjusted according to requirements;
s2, solution sampling: pouring biological solution into the liquid transfer tank, inserting the pipette head into the liquid transfer tank, pulling a sliding block on the handheld automatic liquid preparation gun to drive the lead rod 2 to move in the cavity of the pipette rod along the axial direction of the pushing pipette rod 1, and driving a piston rod 61 in the lead component 6 to move in the piston cylinder 9 in the direction away from the pipette head 10 when the lead rod 2 moves so that the pipette head 10 sucks solution with the volume of xul;
s3, liquid dropping: opening a cover of the culture dish, aligning the handheld automatic liquid preparation gun to the center of the culture dish, then pushing a lead rod 2 on the handheld automatic liquid preparation gun to move towards the direction close to a pipette tip 10 for liquid dropping, driving a piston rod 61 in a lead component 6 to move when the lead rod 2 moves so as to enable the solution in the pipette tip 10 to drop into the culture dish, adding mineral oil into the culture dish and then putting the culture dish into an incubator to complete liquid preparation operation, wherein the handheld automatic liquid preparation gun is used for dropping one or more culture dishes by one-time liquid absorption;
s4, separating the pipette tip 10: after completion of the instillation of the solution, the pipette tip 10 is detached from the piston cylinder 9.
Since the gun withdrawal sleeve (5) is arranged in the embodiment, the step S4 of detaching the pipette head (10) comprises the following steps: after the solution instillation is finished, one or more pipette tips (10) are ejected simultaneously by sliding the gun withdrawal sleeve (5).
Step S3 of this embodiment may push the slider in reverse to complete the dripping while dripping.
Example 2
In this embodiment, the front end cover 7 and the lead cylinder 4 are of an integral structure, so that the installation steps of the front end cover 7 and the lead cylinder 4 are saved.
Example 3
Only the difference from the above embodiment is described in this embodiment, and other technical features are the same, in this embodiment, in another embodiment, the gun withdrawing sleeve 5 may be connected to one end of the front end cover 7 away from the lead cylinder 4, and the elastic member in the elastic assembly 52 is disposed between one end of the gun withdrawing sleeve 5 connected to the front end cover 7 and one end of the front end cover 7 away from the lead cylinder 4, when the gun withdrawing sleeve 5 is pushed, the gun withdrawing sleeve 5 stretches the elastic member, so that the gun withdrawing sleeve 5 moves towards the pipette tip 10 and pushes the pipette tip 10 away from the piston cylinder 9, and after the pipette tip 10 is separated from the piston cylinder 9, only the pushing force applied to the gun withdrawing sleeve 5 needs to be cancelled, so that the elastic member recovers its deformation, and the gun withdrawing sleeve 5 is reset by the pulling force of the elastic member.
Example 4
In this embodiment, only the difference from the above-described embodiment is described, and other technical features are the same, and in this embodiment, the lead rod 2 may be directly connected to the lead plate 62.
Example 5
Only differences from the above embodiments are described in this embodiment, other technical features are the same, in this embodiment, the handheld automatic liquid preparation gun further includes a piston cylinder pressing plate 8, a pressing groove for installing the piston cylinder pressing plate 8 is formed in one end, facing the piston cylinder 9, of the front end cover 7, the piston cylinder pressing plate 8 is installed in the pressing groove, a fixing hole for inserting the piston cylinder 9 is formed in the front end cover 7, as shown in fig. 8, a clamping ring 91 is arranged at one end, inserted into the front end cover 7, of the piston cylinder 9, the outer diameter of the clamping ring 91 is larger than the inner diameter of the fixing hole, and the piston cylinder pressing plate 8 is used for pressing the clamping ring 91 in the pressing groove of the front end cover 7. The arrangement can enable the snap ring 91 to be clamped at one side of the front end cover 7 facing the lead cylinder 4 and abut against the snap ring 91 at the end part of the piston cylinder 9, so that the piston cylinder pressing plate 8 can press the piston cylinder 9 on the front end cover 7, and the piston cylinder 9 is prevented from shaking during liquid suction.
Example 6
Only the difference from the above embodiment is described in this embodiment, and other technical features are the same, in this embodiment, a pipetting button 13 and a liquid amount adjusting gear are disposed at one end of the pushing gun rod 1 away from the connecting cover 3, one end of the pipetting button 13 extends into the gun rod cavity of the pushing gun rod 1 to be connected with the lead rod 2, and the pipetting button 13 pushes the lead rod 2 to complete the liquid dropping. Liquid measure adjusting gear with move liquid button 13 and connect, set up a plurality of gears on the liquid measure adjusting gear, through the rotation liquid measure adjusting gear adjusts the dropping liquid volume at every turn, liquid measure adjusting gear can adjust and press the produced orientation of liquid-transfering button 13 at every turn the distance of removal in the barral of a gun cavity, and then adjust move liquid button 13 promotes the distance that 2 mobile of lead pole, finally accomplishes the accurate control of liquid drop volume, therefore the xul capacity solution of absorbing in the pipette tip 10 in this embodiment can come discharge once or many times through adjusting liquid measure adjusting gear.
Specifically, the liquid amount adjusting gear can be set with 5 gears, the liquid amount adjusting gear corresponds to the liquid drops with the liquid amounts of (3 ul,6ul,9ul,12ul and 15ul), and the liquid drop with the corresponding volume can be pushed out by pressing the pipetting button 13 through rotating the liquid amount adjusting gear.
The sampling dropping method of the handheld liquid preparation gun of the embodiment is as follows:
s1, mounting a pipette tip 10: butting a piston cylinder 9 of a handheld automatic liquid preparation gun with a liquid transfer gun head 10, wherein the liquid transfer gun head 10 is connected with one end, far away from the front end cover 7, of the piston cylinder 9, so that the liquid transfer gun head 10 is firmly sleeved on the piston cylinder 9, and the liquid absorption range of the liquid preparation gun is adjusted according to requirements;
s2, solution sampling: pouring biological solution into the liquid transfer tank, inserting the pipette head into the liquid transfer tank, pulling a sliding block on the handheld automatic liquid preparation gun to drive the lead rod 2 to move in the cavity of the pipette rod along the axial direction of the pushing pipette rod 1, and driving a piston rod 61 in the lead component 6 to move in the piston cylinder 9 in the direction away from the pipette head 10 when the lead rod 2 moves so that the pipette head 10 sucks solution with the volume of xul;
s3, liquid dropping: aligning a handheld automatic liquid preparation gun to the center of a culture dish, adjusting the gear of a liquid quantity adjusting gear according to the volume requirement of actually required liquid drops, then pressing a liquid transfer button 13 to push a lead rod 2 to move towards the direction close to a liquid transfer gun head 10 so as to enable a solution to be dripped into the culture dish, wherein the volume of the liquid drop discharged each time is determined by the gear of the liquid quantity adjusting gear, the solution with the volume of xul is discharged for one time or multiple times, then adding mineral oil and then placing the solution into a culture box to complete liquid preparation operation so that the handheld automatic liquid preparation gun can drip one or more culture dishes by one-time liquid suction;
s4, separating the pipette tip 10: after the solution is instilled, the pipette tips 10 are simultaneously ejected by sliding the withdrawal sleeve 5.
The embodiment of the sample liquid detection card provided by the invention is explained in detail above. The principles and embodiments of the present invention have been described herein using specific examples, which are presented only to assist in understanding the core concepts of the present invention. It should be noted that, for those skilled in the art, without departing from the principle of the present invention, the present invention can also make several improvements and modifications, and those improvements and modifications also fall into the protection scope of the present claims.
Claims (10)
1. The sampling dropping method is characterized in that the automatic liquid preparation gun comprises a push gun rod (1), a lead rod (2), a connecting cover (3), a lead cylinder (4), a lead component (6), a front end cover (7) and a liquid transfer gun head (10), a gun rod cavity is axially arranged on the push gun rod (1), the lead rod (2) is arranged in the gun rod cavity, one side of the connecting cover (3) is connected with one end of the push gun rod (1), the lead cylinder (4) is provided with a lead cavity, one end of the lead cylinder (4) is connected with the other side of the connecting cover (3), one end of the lead rod (2) penetrates through the connecting cover (3) and extends into the lead cavity, the lead component (6) is arranged in the lead cavity of the lead cylinder (4), the lead component (6) comprises a plurality of piston rods (61), the piston rods (61) are arranged at one end of the lead rod (2) departing from one end of the lead cylinder (4) so as to push the lead rod (2) to push the piston rod (2) to move in the lead cavity, the front end cover (7) is connected with the front end cover (7), and the piston rod (61) is arranged on the front end cover (7), and the piston rod (7), so that the piston rod (61) can be inserted into the corresponding piston cylinder (9) in a matching way;
the sampling dropping method comprises the following steps:
s1, mounting a liquid transfer gun head (10): butt-jointing a piston cylinder (9) of a handheld automatic liquid preparation gun with a liquid transfer gun head (10), wherein the liquid transfer gun head (10) is connected with one end, far away from the front end cover (7), of the piston cylinder (9) so that the liquid transfer gun head (10) is firmly sleeved on the piston cylinder (9), and the liquid absorption range of the liquid preparation gun is adjusted according to requirements;
s2, solution sampling: pouring biological solution into a liquid transfer groove, inserting a gun head into the liquid transfer groove, pulling a lead rod (2) on a handheld automatic liquid preparation gun to move in a gun rod cavity along the axial direction of a push gun rod (1), and driving a piston rod (61) in a lead assembly (6) to move in a direction far away from a liquid transfer gun head (10) in a piston cylinder (9) when the lead rod (2) moves so that the liquid transfer gun head (10) absorbs solution with the volume of xul;
s3, liquid dropping: opening a cover of the culture dish, aligning the handheld automatic liquid preparation gun to the center of the culture dish, then pushing a lead rod (2) on the handheld automatic liquid preparation gun to move towards the direction close to a liquid transfer gun head (10) for liquid dropping, driving a piston rod (61) in a lead component (6) to move when the lead rod (2) moves so as to enable a solution in the liquid transfer gun head (10) to drop into the culture dish, adding mineral oil into the culture dish and then placing the culture dish into a culture box to complete liquid preparation operation, wherein the handheld automatic liquid preparation gun is used for dropping one or more culture dishes by liquid absorption at one time;
s4, separating the pipette tip (10): and after the solution instillation is finished, the pipette tip (10) is separated from the piston cylinder (9).
2. The sampling and dripping method of the hand-held automatic liquid preparation gun according to the claim 1 is characterized in that the hand-held automatic liquid preparation gun is provided with a liquid transferring button (13) and a liquid quantity adjusting gear at one end of the pushing gun rod (1) far away from the connecting cover (3), one end of the liquid transferring button (13) extends into the gun rod cavity of the pushing gun rod (1) to be connected with the lead rod (2), and the liquid is dripped by pushing the lead rod (2) through the liquid transferring button (13); liquid measure adjusting gear with move liquid button (13) and connect, set up a plurality of gears on the liquid measure adjusting gear, through rotatory liquid measure adjusting gear adjusts at every turn the dropping liquid volume, liquid measure adjusting gear is used for adjusting and presses the produced orientation of liquid-moving button (13) at every turn the distance of removal in the barral of a gun cavity, and then adjusts move liquid button (13) and promote the distance that lead pole (2) moved.
3. The sampling and dripping method for a hand-held automatic liquid preparation gun according to claim 2, wherein the step S3 of pushing the lead rod (2) of the hand-held automatic liquid preparation gun for dripping further comprises the following steps: the gear of the liquid quantity adjusting gear is adjusted according to the volume requirement of actually required liquid drops, then a liquid transfer button (13) is pressed to push a lead rod (2) to enable solution to be dripped into a vessel, the volume of the liquid drops discharged each time is determined by the gear of the liquid quantity adjusting gear, the solution with the volume of xul is discharged one or more times, then mineral oil is added, and then the solution is placed into an incubator to complete liquid preparation operation, so that one or more culture vessels can be dripped by one-time liquid suction of a handheld automatic liquid preparation gun.
4. A method for taking samples from a hand-held automatic liquid preparation gun according to any one of claims 1-3, characterized in that the hand-held automatic liquid preparation gun further comprises a gun withdrawal sleeve (5), one end of the gun withdrawal sleeve (5) is connected with the front end cap (7) or the lead cylinder (4), and the other end of the gun withdrawal sleeve (5) is provided with a through hole for the piston cylinder (9) to pass through, so that the end of the piston cylinder (9) far away from the front end cap (7) extends out of the through hole to be connected with the pipette tip (10).
5. A method of sampling dripping according to claim 4 wherein the step S4 of detaching the pipette tip (10) comprises the steps of: after the solution instillation is finished, one or more pipette tips (10) are ejected simultaneously by sliding the gun withdrawal sleeve (5).
6. The method for sampling and dripping of a hand-held automatic liquid preparation gun according to claim 4, characterized in that the gun withdrawal sleeve (5) is movably connected with the front end cover (7) or the lead cylinder (4), and the gun withdrawal sleeve (5) can reciprocate relative to the front end cover (7) or the lead cylinder (4) so that the end of the gun withdrawal sleeve (5) provided with the perforation pushes the pipette tip (10) to separate the pipette tip (10) from the piston cylinder (9).
7. A method of dispensing a sample from a hand-held automatic liquid gun according to claim 4, characterized in that the withdrawal sleeve (5) is connected to the front end cap (7) by a spring assembly (52), and the spring in the spring assembly (52) is compressed or stretched by pushing the withdrawal sleeve (5) to move the withdrawal sleeve (5) relative to the front end cap (7) and to separate the pipette tip (10) from the piston cylinder (9).
8. The sampling and dripping method of the hand-held automatic liquid preparation gun according to claim 7, characterized in that the elastic component (52) comprises a spring element (521), a spring pressing block (522) and a fastening bolt (523), the spring pressing block (522) is fixed at the end of the gun backing sleeve (5) through the fastening bolt (523), the front end cover (7) is provided with a mounting hole for assembling the spring element (521), one end of the spring element (521) is fixed in the mounting hole, and the other end of the spring element (521) is connected with the spring pressing block (522).
9. A method for sampling and dispensing a liquid from a hand-held automatic liquid gun according to claim 1, characterized in that the lead assembly (6) further comprises a lead plate (62), a groove for fixing the piston rod (61) is provided at an end of the lead plate (62) facing away from the connection cap (3), and an end of the lead plate (62) facing the connection cap (3) is connected to the lead rod (2).
10. The sampling dropping method of the hand-held automatic liquid preparation gun is characterized in that a sliding block is arranged at one end of the lead rod (2) facing the pushing gun rod (1), a side groove (11) for the sliding block to extend out is formed in the side wall of the pushing gun rod (1), and the lead rod (2) moves in the gun rod cavity by pushing the sliding block.
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| CN202211043248.7A CN115283035B (en) | 2022-08-29 | 2022-08-29 | Sampling and dripping method of hand-held automatic liquid preparation gun |
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| CN202211043248.7A CN115283035B (en) | 2022-08-29 | 2022-08-29 | Sampling and dripping method of hand-held automatic liquid preparation gun |
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