CN115282185B - Fermented salvia miltiorrhiza extract containing salvia miltiorrhiza enzymes, and preparation method and application thereof - Google Patents
Fermented salvia miltiorrhiza extract containing salvia miltiorrhiza enzymes, and preparation method and application thereof Download PDFInfo
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/537—Salvia (sage)
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
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Abstract
The invention belongs to the technical field of plant extract medicine research, and particularly relates to a salvia miltiorrhiza-enzyme-containing fermented salvia miltiorrhiza extract with a blood pressure reducing effect, and a preparation method and application thereof. The fermented red sage root extract is fermented by using the Dan-participated grass jade dew as a fermentation base material and using the probiotics to prepare the ferment product with good blood pressure reducing effect, and can be used for preparing blood pressure reducing medicines. Compared with the red sage root enzyme extract, the mixed enzyme extract of the red sage root and the grass jade dew has better blood pressure reducing effect, and the side surface proves that the grass jade dew enzyme extract has the blood pressure reducing effect and can be used as a potential blood pressure reducing substance for development and research.
Description
Technical Field
The invention belongs to the technical field of plant extract medicine research, and particularly relates to a fermented salvia miltiorrhiza extract containing salvia miltiorrhiza enzymes, and a preparation method and application thereof.
Background
The red sage root (Salvia miltiorrhiza Bge.) belongs to dicotyledonous Labiatae plant, is one of the most commonly used traditional Chinese medicines for activating blood and dissolving stasis, and has the effects of removing stasis and relieving pain, nourishing blood and tranquillizing. The traditional medicinal part of Saviae Miltiorrhizae radix is root, and the above-ground stem and leaf part is generally discarded for administration and application. Research shows that the red sage root contains rich active chemical matters, such as salvianolic acid, tanshinone, saccharide, etc. and has the biological activities of resisting virus, tumor, bacteria, inflammation, oxidation, etc.
At present, in the functional foods containing the red sage root in the market, the red sage root raw material is mostly crushed and added directly or only simply extracted, so that the effective utilization rate of the red sage root is greatly reduced. The ferment is a mixed fermentation liquor which is produced by taking fresh vegetables, fruits, brown rice, medicinal and edible traditional Chinese medicines and the like as raw materials, extracting or extracting a series of processes, adding yeast, lactobacillus and other fermentation strains for fermentation, and contains rich nutrient components such as sugar, organic acid, mineral substances, vitamins, phenols, terpenes and other bioactive substances such as important enzymes and the like. At present, a process for preparing the red sage root ferment based on the red sage root is developed, more red sage root ferment products are expected to be developed, and the method has positive significance for developing functional products of the red sage root.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to provide the fermented salvia miltiorrhiza bunge extract containing the salvia miltiorrhiza bunge ferment, and the fermented extract has the advantage of rich nutrition;
the second technical problem to be solved by the invention is to provide a preparation method of the fermented salvia miltiorrhiza extract containing the salvia miltiorrhiza ferment;
The third technical problem to be solved by the invention is to provide the application of the fermented salvia miltiorrhiza extract containing the salvia miltiorrhiza enzyme in preparing medicaments for preventing and treating cardiovascular diseases, in particular to the application in preparing medicaments for lowering blood pressure.
In order to solve the technical problems, the preparation method of the fermented salvia miltiorrhiza extract containing the salvia miltiorrhiza enzyme comprises the following steps:
(1) Respectively pulverizing Saviae Miltiorrhizae radix to obtain Saviae Miltiorrhizae radix powder, and pulverizing grass jade dew leaves to obtain grass jade dew broken material;
(2) Mixing the red sage powder and the grass jade dew crushed materials, and adding white sugar and water for uniform mixing to obtain a fermentation base material;
(3) And adding a probiotic agent into the fermentation base material for sealed constant-temperature fermentation, collecting fermentation products for solid-liquid separation, and collecting filtrate to obtain the fermentation base material.
Specifically, in the step (2), the mass ratio of the salvia miltiorrhiza powder to the grass jade dew crushed material to the white sugar to the water is 5-10:1-5:10-15:100.
Specifically, in the step (3), the probiotic agent includes at least one of lactobacillus plantarum, lactobacillus casei, lactobacillus paracasei, lactobacillus acidophilus, or lactobacillus reuteri.
Specifically, the concentration of the freeze-dried powder of the probiotics is 10 9-1010 CFU/g.
Specifically, the probiotic includes a mixture of lactobacillus plantarum and lactobacillus casei.
Specifically, the mass ratio of the lactobacillus plantarum to the lactobacillus casei is 1-2:1.
Specifically, in the step (3), the inoculation amount of the probiotics based on the fermentation base stock is 0.3-0.5wt%.
Specifically, the temperature of the sealed constant temperature fermentation step is 32-38 ℃ and the fermentation time is 20-30 days.
The invention also discloses a fermented red sage root extract containing red sage root ferment prepared by the method.
The invention also discloses application of the salvia miltiorrhiza extract containing the salvia miltiorrhiza ferment in preparing antihypertensive drugs.
The invention also discloses application of the grass jade dew and the fermented extract thereof in preparing antihypertensive drugs.
The fermented red sage root extract is fermented by using the Dan-participated grass jade dew as a fermentation base material and using the probiotics to prepare the ferment product with good blood pressure reducing effect, and can be used for preparing blood pressure reducing medicines. Compared with the red sage root enzyme extract, the mixed enzyme extract of the red sage root and the grass jade dew has better blood pressure reducing effect, and the side surface proves that the grass jade dew enzyme extract has the blood pressure reducing effect and can be used as a potential blood pressure reducing substance for development and research.
Detailed Description
In the following examples of the invention, the probiotic is selected from commercial Lactobacillus hansen (Hansen) plant UALp-05 TM and Lactobacillus casei UALc-03 TM lyophilized powder, wherein the concentration of the lyophilized powder is 10 9-1010 CFU/g. When in use, the stored freeze-dried powder is directly put into the fermentation base material.
Example 1
The preparation method of the fermented salvia miltiorrhiza extract containing the salvia miltiorrhiza enzyme comprises the following steps:
(1) Taking good-quality red sage root, conventionally drying and crushing to obtain red sage root powder for later use; the grass jade dew leaves with good quality and thick leaves are cleaned and then crushed to obtain grass jade dew crushed materials for standby;
(2) Mixing the red sage root powder, the broken grass jade dew, white sugar and water according to the proportion of 8:3:12:100 mass ratio, and fully stirring and uniformly mixing to obtain a fermentation base material;
(3) Adding mixed bacteria (mass ratio of 2:1) of lactobacillus plantarum and lactobacillus casei into the fermentation base material according to the inoculation amount of 0.3 weight percent, and fermenting for 30 days at a constant temperature in a sealed manner at 35 ℃; after fermentation, collecting fermentation products, fully squeezing, filtering, separating and removing substances such as thalli, and collecting filtrate.
Example 2
The preparation method of the fermented salvia miltiorrhiza extract containing the salvia miltiorrhiza enzyme comprises the following steps:
(1) Taking good-quality red sage root, conventionally drying and crushing to obtain red sage root powder for later use; the grass jade dew leaves with good quality and thick leaves are cleaned and then crushed to obtain grass jade dew crushed materials for standby;
(2) Mixing the red sage root powder, the broken grass jade dew, white sugar and water according to a proportion of 5:5:10:100 mass ratio, and fully stirring and uniformly mixing to obtain a fermentation base material;
(3) Adding mixed bacteria (mass ratio is 1:1) of lactobacillus plantarum and lactobacillus casei into the fermentation base material according to the inoculation amount of 0.4 weight percent, and fermenting for 30 days at a constant temperature in a sealed way under the environment of 35 ℃; after fermentation, collecting fermentation products, fully squeezing, filtering, separating and removing substances such as thalli, and collecting filtrate.
Example 3
The preparation method of the fermented salvia miltiorrhiza extract containing the salvia miltiorrhiza enzyme comprises the following steps:
(1) Taking good-quality red sage root, conventionally drying and crushing to obtain red sage root powder for later use; the grass jade dew leaves with good quality and thick leaves are cleaned and then crushed to obtain grass jade dew crushed materials for standby;
(2) Mixing the red sage root powder, the broken grass jade dew, white sugar and water according to the proportion of 10:1:15:100 mass ratio, and fully stirring and uniformly mixing to obtain a fermentation base material;
(3) Adding mixed bacteria (mass ratio of 3:2) of lactobacillus plantarum and lactobacillus casei into the fermentation base material according to the inoculation amount of 0.5 weight percent, and fermenting for 30 days at a constant temperature under a sealed condition at 35 ℃; after fermentation, collecting fermentation products, fully squeezing, filtering, separating and removing substances such as thalli, and collecting filtrate.
Comparative example 1
The preparation method of the fermented red sage root extract of the present comparative example is the same as that of example 1, except that the fermentation is performed only with the red sage root without adding the grass jade dew, and the fermentation conditions are exactly the same.
Comparative example 2
The preparation method of the fermentation extract of this comparative example is the same as that of example 1, except that the fermentation is performed only with the grass cutting dew without adding the root of red-rooted salvia, and the fermentation conditions are exactly the same.
Experimental example
1. Antihypertensive effect on spontaneous hypertensive rats
According to the traditional experimental method, the effect of single oral administration and multiple oral administration for 3 consecutive days on Spontaneous Hypertension Rat (SHR) blood pressure is studied.
Test animals: 4 normal Wistar rats, each half of male and female rats, 3-4 months old, and weight of 200-220g; 24 spontaneous hypertensive rats, each half male, 3-4 months old, and 200-220g body weight were purchased from the Fu-external cardiovascular disease hospital laboratory animal center of the national academy of sciences of Beijing, china.
Feeding: raising in positive pressure purified and ventilated animal house, and free feeding and drinking water at room temperature of 25+ -2deg.C.
Test instrument: CRS-III type computer rat heart rate sphygmomanometer, 101-2 electric heating incubator.
Experimental grouping:
Normal control group: normal Wistar rats (n=4), normal rearing, automatic drinking, no treatment;
Blank control group: spontaneous hypertensive rats (n=4);
Test drug group 1-5: spontaneous hypertensive rats (n=4) and male and female halves were each set up.
The test method comprises the following steps:
After purchase of the test animals, rats were placed in a load cell for training for 2 weeks and blood pressure was measured every other day from the second week. The animals were warmed in an electric oven at 37.+ -. 1 ℃ before pressure measurement and after 15 minutes were assayed for rat tail arterial systolic pressure (SBP) and diastolic pressure (DBP) using a CRS-III computer heart rate blood pressure monitor.
Normal control group: normal Wistar rats were fed with normal water, were drinking water automatically, and were not treated at all;
Blank control group: the test rats are commonly bred and filled with distilled water;
test drug group 1-3: the fermented extracts obtained in example 1 and comparative examples 1-2 were administered to test rats at a dose of 50mg/kg body weight, respectively.
The rats tested in the negative control group and the test drug groups 1 to 5 were dosed once daily for 3 consecutive days, and blood pressure was measured 4 hours after dosing before the test, the second day (48 h) and the third day (72 h), respectively.
Experimental results: the test data are expressed as mean ± standard deviation, and the test results are shown in table 1 below.
Table 1 Effect of each experimental group on spontaneous hypertension rat blood pressure
Therefore, the fermentation extract containing the red sage root ferment has the advantages of quick acting time, quick blood pressure reduction effect and good blood pressure reduction persistence. The results of each experimental group show that the ferment-containing fermented extract obtained based on the red sage root and the grass jade dew as the fermentation base materials has obviously better blood pressure reducing performance than the fermented extract obtained based on the red sage root or the grass jade dew alone. In particular, it should be emphasized that the above experiments prove that the fermented extract containing the grass-leaved crab apple ferment has the effect of reducing blood pressure, and the effect of reducing blood pressure alone is slightly inferior to that of the red sage root ferment, but can be used as a potential blood pressure reducing substance for subsequent researches.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (5)
1. A preparation method of a fermented red sage root extract containing red sage root ferment with blood pressure reducing effect is characterized by comprising the following steps:
(1) Respectively pulverizing Saviae Miltiorrhizae radix to obtain Saviae Miltiorrhizae radix powder, and pulverizing grass jade dew leaves to obtain grass jade dew broken material;
(2) Mixing the red sage powder and the grass jade dew crushed materials, and adding white sugar and water for uniform mixing to obtain a fermentation base material;
(3) Adding a probiotic agent into the fermentation base material for sealed constant-temperature fermentation, collecting fermentation products for solid-liquid separation, and collecting filtrate to obtain the fermentation base material;
the probiotics comprise at least one of lactobacillus plantarum, lactobacillus casei, lactobacillus paracasei, lactobacillus acidophilus or lactobacillus reuteri;
In the step (2), the mass ratio of the red sage root powder, the grass jade dew crushed material, the white sugar and the water is 5-10:1-5:10-15:100;
The concentration of the freeze-dried powder of the probiotics is 10 9-1010 CFU/g;
In the step (3), the inoculation amount of the probiotics based on the fermentation base stock is 0.3-0.5wt%;
The temperature of the sealed constant temperature fermentation step is 32-38 ℃ and the fermentation time is 20-30 days.
2. The method for preparing a fermented red sage root extract containing red sage root ferment according to claim 1, wherein the probiotic comprises a mixture of lactobacillus plantarum and lactobacillus casei.
3. The method for preparing the fermented salvia miltiorrhiza extract containing the salvia miltiorrhiza enzyme according to claim 2, wherein the mass ratio of the lactobacillus plantarum to the lactobacillus casei is 1-2:1.
4. A fermented red sage extract containing a red sage enzyme prepared by the method of any one of claims 1 to 3.
5. The use of the fermented salvia miltiorrhiza extract containing the salvia miltiorrhiza enzyme according to claim 4 for preparing a antihypertensive drug.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1844363A (en) * | 2006-04-21 | 2006-10-11 | 江南大学 | Lactobacillus plantarum CW006 with antihypertensive function |
| CN104012955A (en) * | 2013-12-20 | 2014-09-03 | 湖北文理学院 | Production method of salvia miltiorrhiza enzyme |
| CN106666703A (en) * | 2016-07-11 | 2017-05-17 | 佛山市朗达信息科技有限公司 | Enzyme composition with liver-protecting function, as well as preparation and preparation method thereof |
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| CN1844363A (en) * | 2006-04-21 | 2006-10-11 | 江南大学 | Lactobacillus plantarum CW006 with antihypertensive function |
| CN104012955A (en) * | 2013-12-20 | 2014-09-03 | 湖北文理学院 | Production method of salvia miltiorrhiza enzyme |
| CN106666703A (en) * | 2016-07-11 | 2017-05-17 | 佛山市朗达信息科技有限公司 | Enzyme composition with liver-protecting function, as well as preparation and preparation method thereof |
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