CN115248606A - Control method of gas supply system and gas supply system - Google Patents
Control method of gas supply system and gas supply system Download PDFInfo
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- CN115248606A CN115248606A CN202210749014.8A CN202210749014A CN115248606A CN 115248606 A CN115248606 A CN 115248606A CN 202210749014 A CN202210749014 A CN 202210749014A CN 115248606 A CN115248606 A CN 115248606A
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- 238000000034 method Methods 0.000 title claims abstract description 48
- 229910000831 Steel Inorganic materials 0.000 claims abstract description 102
- 239000010959 steel Substances 0.000 claims abstract description 102
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- 230000010261 cell growth Effects 0.000 abstract description 10
- 239000007789 gas Substances 0.000 description 462
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 24
- 229910002092 carbon dioxide Inorganic materials 0.000 description 12
- 239000001569 carbon dioxide Substances 0.000 description 12
- 230000008859 change Effects 0.000 description 12
- 239000002699 waste material Substances 0.000 description 9
- 238000001514 detection method Methods 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
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- G—PHYSICS
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- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D11/00—Control of flow ratio
- G05D11/02—Controlling ratio of two or more flows of fluid or fluent material
- G05D11/13—Controlling ratio of two or more flows of fluid or fluent material characterised by the use of electric means
- G05D11/139—Controlling ratio of two or more flows of fluid or fluent material characterised by the use of electric means by measuring a value related to the quantity of the individual components and sensing at least one property of the mixture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention relates to the technical field of biological culture equipment, in particular to a control method of a gas supply system and the gas supply system, and aims to solve the problem that the conventional biological incubator cannot switch between a main gas supply steel cylinder and a standby gas supply steel cylinder in time according to the concentration of gas in the incubator. Therefore, the gas supply system comprises an incubator, a main gas supply steel cylinder and a standby gas supply steel cylinder, and the control method comprises the following steps: obtaining the gas concentration C in the incubator 1 (ii) a According to gas concentration C 1 And standard concentration C 0 And selectively switching to supply gas to the incubator by using a spare gas supply steel cylinder. Through such setting, can in time carry out main air feed steel according to gas concentration and standard concentration in the incubatorThe switching between the bottle and the standby gas supply steel bottle prevents the gas supply of the gas supply system to the incubator from being interrupted due to the untimely switching of the main gas supply steel bottle, thereby avoiding influencing the growth of cells and tissues in the incubator; simultaneously, can prevent to cause gas extravagant because of main gas supply steel bottle switches too early.
Description
Technical Field
The invention relates to the technical field of biological culture equipment, and particularly provides a control method of an air supply system and the air supply system.
Background
The biological incubator is a device for in vitro culture by simulating the formation of a similar cell and tissue in the incubator of the incubator.
Taking a carbon dioxide incubator as an example, the carbon dioxide incubator can provide an appropriate temperature and carbon dioxide concentration for the growth of cells, tissues, and the like. Wherein, carbon dioxide gas is provided by external gas steel bottle, and external gas steel bottle generally includes main gas supply steel bottle and reserve gas supply steel bottle, and when the carbon dioxide in main gas supply steel bottle used up, need switch to and carry out the gas feed for the carbon dioxide incubator by reserve gas supply steel bottle.
The existing biological incubator can not timely switch the main gas supply steel cylinder and the standby gas supply steel cylinder according to the concentration of gas in the biological incubator, thereby causing the waste of gas in the steel cylinders or influencing the growth of cells and tissues in the incubator.
Therefore, there is a need in the art for a new solution to the above-mentioned problems.
Disclosure of Invention
The present invention is intended to solve the above-mentioned problems, that is, to solve the problem that the conventional biological incubator cannot switch between the main gas supply cylinder and the backup gas supply cylinder in time according to the gas concentration in the biological incubator.
In a first aspect, the present invention provides a method of controlling a gas supply system including an incubator, a main gas supply cylinder, and a backup gas supply cylinder, the method comprising: obtaining the gas concentration C in the incubator 1 (ii) a According to the gas concentration C 1 And standard concentration C 0 And selectively switching to the spare gas supply steel cylinder to supply gas to the incubator.
In a preferred embodiment of the above control method, "based on the gas concentration C 1 And standard concentration C 0 The specific steps of selectively switching to the supply of gas from the spare gas supply cylinder to the incubator comprise: calculating a first difference Δ 1 ＝C 0 -C 1 (ii) a The first difference delta is calculated 1 With a first predetermined value A 1 Comparing; and selectively switching to supply gas to the incubator from the standby gas supply steel cylinder according to the comparison result.
In a preferred embodiment of the control method, the step of selectively switching to supply gas from the backup gas supply cylinder to the incubator according to the comparison result includes: if Δ 1 ≤A 1 If the culture box is not switched to the spare gas supply steel cylinder, the culture box is not supplied with gas; if Δ 1 ＞A 1 Reaches a first preset time T 1 Then further judging that the time is at the second preset time T 2 Whether the concentration of gas in the incubator is increased; and selectively switching to supply gas to the incubator by the spare gas supply steel cylinder according to the judgment result.
In a preferred embodiment of the control method, the step of selectively switching to supply gas from the backup gas supply cylinder to the incubator according to the determination result includes: if the judgment result is yes, the spare gas supply steel cylinder is not switched to supply gas to the incubator; if the judgment result is 'no', increasing the gas supply amount of the main gas supply steel cylinder, and further judging the third preset time T 3 Whether the concentration of gas in the incubator is increased; and selectively switching to supply gas to the incubator by the spare gas supply steel cylinder according to the judgment result.
In a preferred embodiment of the control method, the step of selectively switching to supply gas from the backup gas supply cylinder to the incubator according to the determination result includes: if the judgment result is yes, the spare gas supply steel cylinder is not switched to supply gas to the incubator; if the judgment result is 'no', the spare gas supply steel cylinder is switched to supply gas to the incubator.
In a preferred technical solution of the above control method, the gas supply system further includes an alarm device, and in a case where the determination result is "no", the control method further includes: and starting the alarm device.
In a preferred embodiment of the above control method, the step of increasing the gas supply amount of the main gas supply cylinder includes: and adjusting the opening degree of the valve of the main gas supply steel cylinder to the maximum opening degree.
In a preferred embodiment of the control method, the control method of the present invention further includes: if a is equal 1 ＞A 1 Reaches a fourth preset time T 4 And when the culture box is used, the culture box is switched to be supplied with gas by the standby gas supply steel cylinder.
In a preferred embodiment of the above control method, "determining is performed for the second preset time T 2 The specific steps of whether the gas concentration in the incubator is increased or not "include: calculating a second difference Δ 2 ＝C i+n -C i (ii) a The second difference Delta 2 With a second predetermined value A 2 Carrying out comparison; if Δ 2 ＞A 2 Then, it is determined as being in the second preset time T 2 Whereby the concentration of gas in said incubator increases; wherein, C i The gas concentration, C, in the incubator obtained at the beginning of the judgment i+n The gas concentration in the incubator obtained at every n set times after the start of judgment.
In a second aspect, the present invention provides a gas supply system comprising a controller configured to be able to perform the control method described above.
Under the condition of adopting the technical scheme, the gas supply system can selectively switch to supply gas to the incubator from the standby gas supply steel cylinder through the gas concentration and the standard concentration, so that the gas supply system can switch the supply of the gas to the incubator from the main gas supply steel cylinder to the supply of the gas to the incubator from the standby gas supply steel cylinder in time according to the gas concentration and the standard concentration in the incubator, and on one hand, the gas supply system can prevent the gas supply system from intermittently supplying gas to the incubator due to untimely switching of the main gas supply steel cylinder, thereby influencing the growth of cells and tissues in the incubator; on the other hand, can prevent to lead to the remaining gas in the main gas supply steel bottle extravagant because of main gas supply steel bottle switches over too early, simultaneously, with set up pressure sensor in the steel bottle, realize according to pressure sensor's numerical value that supply gas by main gas supply steel bottle to the incubator switches into and supplies gas by reserve gas supply steel bottle to the incubator and compare, can save pressure sensor's use to reduce gas supply system's cost.
Further, through calculating the difference of gaseous concentration and standard concentration in the incubator earlier, comparing this difference with first default again, compare with direct gaseous concentration and standard concentration, can avoid causing the erroneous judgement to gaseous concentration in the incubator because of the detection error of gaseous concentration in the incubator to it is more accurate to make the judgement that switches between main gas supply steel bottle and the reserve gas supply steel bottle.
Still further, by comparing the first difference Δ 1 And a first preset value A 1 Comparing, selectively switching to supply the incubator with the spare gas cylinder, on one hand, when delta 1 ≤A 1 When the culture box is not switched to be supplied with gas by the spare gas supply steel cylinder, the difference value between the gas concentration in the culture box and the standard concentration is small, namely, the main gas supply steel cylinder can provide gas for the culture box so that the concentration in the culture box provides gas with proper concentration for the growth of cells and tissues in the culture box, and the gas supply steel is not neededSwitching bottles; on the other hand, when Δ 1 ＞A 1 Reaches a first preset time T 1 Further judging that the time is within a second preset time T 2 Whether the gas concentration in the inner culture box is increased or not, and when only delta occurs 1 ＞A 1 Further determining the time at a second preset time T 2 Comparing the situation that whether the gas concentration in the incubator is increased or not, the duration of the gas concentration in the incubator is monitored to be lower than the standard concentration and exceeds the first preset time T 1 Further judging the time at a second preset time T 2 Whether or not the gas concentration in the incubator rises can be prevented from occurring at a certain timing due to an error in the detection of the gas concentration 1 ＞A 1 The judgment accuracy is improved due to the misjudgment caused by the misjudgment; meanwhile, if Δ 1 ＞A 1 Reaches a first preset time T 1 When the gas concentration in the incubator is lower than the standard concentration for a certain period of time, the gas amount in the main gas cylinder may be insufficient or no gas may be present in the main gas cylinder, and therefore, it is necessary to further determine that the second preset time T is reached 2 The gas concentration in the inner incubator is increased to judge whether the main gas supply steel cylinder is supplying gas for the incubator.
Still further, by judging at the second preset time T 2 Whether the gas concentration in the incubator therein has risen, on the one hand, if it has been for a second predetermined time T 2 When the gas concentration in the incubator rises to some extent, the main gas supply steel cylinder supplies gas to the incubator, namely, residual gas still exists in the main gas supply steel cylinder, so that the incubator is not supplied with gas by a spare gas supply steel cylinder, and waste of the residual gas in the main gas supply steel cylinder is avoided; on the other hand, if at the second preset time T 2 In this case, if the gas concentration in the incubator is not increased, it may be caused by the fact that the main gas cylinder is empty, or, although there is still residual gas in the main gas cylinder, the gas is introduced slowly due to insufficient gas amount, so that the second preset time T is reached 2 In the incubator, the concentration of gas is not increased, and therefore, it is necessary to furtherIncreasing the gas supply amount of the main gas supply cylinder step by step, and detecting the third preset time T 3 Whether the gas concentration in the inner incubator rises, whether the main gas supply steel cylinder can supply gas for the incubator is judged, whether residual gas exists in the main gas supply steel cylinder is further judged, and therefore the waste of residual gas in the main gas supply steel cylinder can be avoided.
Further, according to the judgment result of whether the gas concentration in the incubator is increased within the third preset time, the gas supply of the incubator by the spare gas supply steel cylinder is selectively switched, on one hand, if the gas concentration in the incubator is increased within the third preset time after the gas supply amount of the main gas supply steel cylinder is increased, the gas supply of the incubator by the spare gas supply steel cylinder is not switched at the moment, although the gas amount in the main gas supply steel cylinder is insufficient, residual gas exists, and the waste of the residual gas in the main gas supply steel cylinder is avoided; on the other hand, if the gas concentration in the incubator does not rise for the third predetermined time after the amount of gas supplied from the main gas cylinder is increased, it indicates that there is no gas in the main gas cylinder, and at this time, it is necessary to switch to supply gas from the backup gas cylinder to the incubator, thereby preventing the growth of cells and tissues in the incubator from being affected by insufficient gas supply to the incubator.
Still further, when Δ 1 ＞A 1 Reaches a fourth preset time T 4 When the culture medium is required to be supplied, the culture medium is switched to the spare gas supply cylinder to supply gas to the culture medium, and by such an arrangement, if Δ 1 ＞A 1 Reaches a fourth preset time T 4 When the gas concentration in the incubator is lower than the standard concentration for a long time, the gas supply is switched to the spare gas supply steel cylinder to supply gas to the incubator in time, and the influence on the normal growth of cells and tissues in the incubator due to the fact that the gas concentration in the incubator is lower than the standard concentration for a long time can be avoided.
In addition, the gas supply system further provided on the basis of the technical scheme has the beneficial effects of the control method due to the adoption of the introduced control method, and compared with the gas supply system before improvement, the gas supply system provided by the invention has the advantages that the judgment is more accurate when the main gas supply steel cylinder and the standby gas supply steel cylinder are switched, the switching is more timely, and the gas waste is avoided while the normal growth of cells and tissues in the incubator is not influenced.
Drawings
Preferred embodiments of the present invention are described below with reference to the accompanying drawings, in which:
FIG. 1 is a schematic view of the construction of the air supply system of the present invention;
FIG. 2 is a flow chart of the control method of the present invention;
fig. 3 is a flow chart of an embodiment of the control method of the present invention.
List of reference numerals:
1. an incubator; 11. a gas concentration sensor; 12. an alarm device; 2. a main gas supply steel cylinder; 21. a first pipe; 211. a first control valve; 3. a spare gas supply steel cylinder; 31. a second conduit; 311. a second control valve.
Detailed Description
Preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only for explaining the technical principle of the present invention, and are not intended to limit the scope of the present invention.
It should be noted that in the description of the present invention, the term "inner" is based on the direction or positional relationship shown in the drawings, which is for convenience of description only, and does not indicate or imply that the device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and "fourth" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In addition, it should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "disposed" and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The prior biological incubator pointed out based on the background art cannot timely switch a main gas supply steel cylinder and a standby gas supply steel cylinder according to the gas concentration in the biological incubator. The invention provides a control method of a gas supply system, aiming at switching the gas supply system to supply gas to an incubator from a spare gas supply steel cylinder in time according to the gas concentration in the incubator.
Referring to fig. 1, fig. 1 is a schematic structural diagram of an air supply system according to the present invention.
As shown in fig. 1, the gas supply system of the present invention includes an incubator 1, a main gas supply cylinder 2, and a backup gas supply cylinder 3, both the main gas supply cylinder 2 and the backup gas supply cylinder 3 can supply gas to the incubator 1, and in general, the main gas supply cylinder 2 supplies gas to the incubator 1, and when the gas in the main gas supply cylinder 2 is insufficient, the main gas supply cylinder is automatically switched to the backup gas supply cylinder 3.
Illustratively, carbon dioxide gas is stored in each of the main gas cylinder 2 and the reserve gas cylinder 3, and the main gas cylinder 2 and the reserve gas cylinder 3 are communicated with the incubator 1 through a first pipe 21 and a second pipe 31, respectively. The first pipeline 21 and the second pipeline 31 are respectively provided with a first control valve 211 and a second control valve 311, and the switching between the main gas supply steel cylinder 2 and the standby gas supply steel cylinder 3 is realized by controlling the opening and closing of the first control valve 211 and the second control valve 311.
It should be noted that, in practical applications, the main gas cylinder 2 and the backup gas cylinder 3 described above are not limited to be communicated with the incubator 1 through the first pipeline 21 and the second pipeline 31, respectively, and the switching between the main gas cylinder 2 and the backup gas cylinder 3 is realized by controlling the first control valve 211 and the second control valve 311 provided on the first pipeline 21 and the second pipeline 31, for example, the gas supply ports of the main gas cylinder 2 and the backup gas cylinder 3 may be directly communicated with the incubator 1, the switching between the main gas cylinder 2 and the backup gas cylinder 3 is realized by controlling the control valves on the main gas cylinder 2 and the backup gas cylinder 3, and the like, and such flexible adjustment and change are not departing from the principle and scope of the present invention, and are all included in the protection scope of the present invention. It is of course preferable that switching between the main gas cylinder 2 and the reserve gas cylinder 3 is achieved by controlling the first control valve 211 and the second control valve 311 provided on the first pipe 21 and the second pipe 31.
It should be noted that, in practical applications, those skilled in the art may set both the first control valve 211 and the second control valve 311 as manual valves, or may set both the first control valve 211 and the second control valve 311 as electric control valves, or may set one of the first control valve 211 and the second control valve 311 as a manual valve, and set the other of the first control valve 211 and the second control valve 311 as an electric control valve, etc., and such adjustments and changes to the specific setting types of the first control valve 211 and the second control valve 311 without departing from the principle and scope of the present invention should be included in the protection scope of the present invention.
Preferably, the first control valve 211 and the second control valve 311 are both provided as electrically controlled valves.
The following description will discuss an embodiment of the present invention, taking, as an example, a main gas cylinder 2 supplying gas to an incubator 1 (i.e., the first control valve 211 is open), and a backup gas cylinder 3 as a backup (the second control valve 311 is closed).
Continuing to refer to fig. 2, fig. 2 is a flow chart of a control method of the present invention.
As shown in fig. 2, the control method of the present invention includes the steps of:
s100: obtaining the gas concentration C in the incubator 1 1 ；
S200: according to gas concentration C 1 And standard concentration C 0 The supply of gas from the culture tank 1 to the backup gas cylinder 3 is selectively switched.
Through the arrangement, the culture box 1 can be timely switched from the main gas supply steel cylinder 2 to the spare gas supply steel cylinder 3 according to the gas concentration and the standard concentration in the culture box 1, on one hand, the phenomenon that the main gas supply steel cylinder 2 is not switched timely to enable a gas supply system to supply gas to the culture box 1 intermittently can be prevented, and therefore the growth of cells and tissues in the culture box 1 is influenced; on the other hand, can prevent to lead to the surplus waste in main gas supply steel bottle 2 because of main gas supply steel bottle 2 switches too early, simultaneously, with set up pressure sensor in main gas supply steel bottle 2, realize according to pressure sensor's numerical value that supply gas by main gas supply steel bottle 2 to incubator 1 switches into by reserve gas supply steel bottle 3 to the mode of carrying out the gas supply to incubator 1 and compare, can save pressure sensor's use to reduce gas supply system's cost.
It should be noted that, in practical applications, those skilled in the art may set the supply of gas to the incubator 1 from the main gas supply cylinder 2 and switch the supply of gas to the incubator 1 from the backup gas supply cylinder 3 by closing the control valve on the main gas supply cylinder 2 and opening the control valve on the backup gas supply cylinder 3, or may set the supply of gas to the incubator 1 from the main gas supply cylinder 2 and switch the supply of gas to the incubator 1 from the backup gas supply cylinder 3 by closing the first control valve 211 and opening the second control valve 311, and so on, and such flexible adjustment and change are included in the protection scope of the present invention without departing from the principle and scope of the present invention.
Preferably, switching from the supply of gas from the main gas cylinder 2 to the supply of gas from the backup gas cylinder 3 to the supply of gas from the incubator 1 is performed by closing the first control valve 211 and opening the second control valve 311.
In practical applications, a person skilled in the art may obtain the gas concentration in the incubator 1 by setting a gas concentration sensor in the incubator 1, or may also obtain the gas concentration in the incubator 1 by setting a gas concentration detector in the incubator 1, and the like, and such adjustment and change of the specific obtaining manner of the gas concentration in the incubator 1 are all included in the protection scope of the present invention without departing from the principle and scope of the present invention.
Preferably, as shown in fig. 1, a gas concentration sensor 11 is provided in the incubator 1.
Illustratively, the incubator 1 is a carbon dioxide incubator, the gas is carbon dioxide, and the carbon dioxide concentration in the incubator 1 is obtained from the value of a carbon dioxide concentration sensor provided in the incubator 1.
It should be noted that, in practical applications, the control method of the present invention is not limited to be applied to the supply of carbon dioxide gas, for example, oxygen gas, or nitrogen gas, and the like, and such flexible adjustment and change can be performed without departing from the principle and scope of the present invention, and shall be included in the protection scope of the present invention.
It should be noted that, in practical applications, a person skilled in the art may set the standard concentration to a fixed value, or may set the standard concentration to a set value input by a user, etc., and such flexible adjustment and change are included in the protection scope of the present invention without departing from the principle and scope of the present invention.
Illustratively, the standard concentration is set to a fixed value, such as a standard concentration of 15.0%.
It should be noted that, in practical applications, a person skilled in the art may first calculate a difference between the gas concentration and the standard concentration, and then compare the difference with a preset value, or may first calculate a ratio between the gas concentration and the standard concentration, and then compare the ratio with a preset value, or may directly compare the gas concentration with the standard concentration, and so on, and such flexible adjustment and changes do not depart from the principle and scope of the present invention, and are all included in the protection scope of the present invention.
With continuing reference to fig. 2 and with continuing reference to fig. 3, fig. 3 is a flow chart of an embodiment of the control method of the present invention.
Preferably, as shown in FIGS. 2 and 3, "according to the gas concentration C 1 And standard concentration C 0 The specific steps of selectively switching to the supply of gas from the spare gas cylinder 3 to the incubator 1 include:
s210: calculating a first difference Δ 1 ＝C 0 -C 1 ；
S220: the first difference Delta 1 With a first predetermined value A 1 To carry outComparing;
s230: based on the comparison result, the supply of gas from the spare gas cylinder 3 to the incubator 1 is selectively switched.
By setting the difference between the gas concentration in the incubator 1 and the standard concentration to a first preset value A 1 Comparing, compared with directly comparing the gas concentration with the standard concentration, the misjudgment of the gas concentration in the incubator 1 caused by the detection error of the gas concentration in the incubator 1 can be avoided, so that the judgment of switching between the main gas supply steel cylinder 2 and the standby gas supply steel cylinder 3 is more accurate.
It should be noted that in practical applications, those skilled in the art can set the unit of gas concentration to be percentage, or alternatively, the unit of gas concentration to be ppm, etc., and such flexible adjustment and change of the unit of gas concentration without departing from the principle and scope of the present invention shall be included in the protection scope of the present invention.
Illustratively, the unit of gas concentration is set as a percentage, such as a gas concentration of 15.0%.
It should be noted that the first preset value a can be set by experience or experiment for those skilled in the art 1 The numerical value of (c).
Illustratively, the first preset value is set to 1.0%.
As shown in FIG. 3, the specific steps of "selectively switching to supply gas from the backup gas cylinder 3 to the incubator 1 according to the comparison result" include:
s231: if Δ 1 ≤A 1 If the culture box 1 is not switched to be supplied with gas by the spare gas supply steel cylinder 3;
s232: if Δ 1 ＞A 1 Reaches a first preset time T 1 Then further judging that the time is at the second preset time T 2 Whether the gas concentration in the inner incubator 1 is increased; based on the determination result, the supply of gas from the spare gas cylinder 3 to the incubator 1 is selectively switched.
With such an arrangement, on the one hand, when Δ 1 ≤A 1 When it is saidThe difference between the gas concentration in the incubator 1 and the standard concentration is small, that is, the main gas supply cylinder 2 can supply the gas to the incubator 1 so that the concentration in the incubator 1 can supply the gas with an appropriate concentration for the growth of the cells and tissues in the incubator 1, and at this time, the switching is not necessary.
On the other hand, when 1 ＞A 1 Reaches a first preset time T 1 Further judging the second preset time T 2 Whether or not the gas concentration in the inner culture box 1 is increased and when Δ occurs 1 ＞A 1 Further determining the time at a second preset time T 2 Comparing with the situation that whether the gas concentration in the inner incubator 1 is increased or not, the duration of the gas concentration in the inner incubator 1 lower than the standard concentration exceeds the first preset time T by monitoring 1 Further judging the time at a second preset time T 2 Whether the gas concentration in the incubator 1 is increased or not can be prevented from being increased at a certain time due to an error in the detection of the gas concentration 1 ＞A 1 The judgment accuracy is improved due to misjudgment caused by the misjudgment; meanwhile, if Δ 1 ＞A 1 Reaches a first preset time T 1 When the gas concentration in the incubator 1 is lower than the standard concentration for a certain period of time, the gas amount in the main gas cylinder 2 may be insufficient or the gas may not be present in the main gas cylinder 2, and therefore, it is necessary to further determine that the second preset time T is reached 2 Whether the gas concentration in the inner incubator 1 is increased or not is judged to judge whether the main gas supply cylinder 2 is supplying gas to the incubator 1 or not.
It should be noted that, in practical application, the Δ is not limited to 1 ＞A 1 Reaches a first preset time T 1 Then, further judging that the time is within the second preset time T 2 Whether the gas concentration in the inner culture box 1 is increased or not, for example, may be set so that Δ is present 1 ＞A 1 Then further judging that the time is within the second preset time T 2 Whether the concentration of the gas in the inner culture box 1 is increased, etc., and such flexible adjustment and change do not depart from the principle and scope of the present invention should be included in the protection scope of the present inventionAnd (4) inside.
Of course, it is preferable to set the value of Δ 1 ＞A 1 Reaches a first preset time T 1 Then, further judging that the time is within the second preset time T 2 Whether the gas concentration in the inner culture box 1 is increased.
By such an arrangement, as long as Δ occurs 1 ＞A 1 Then further judging that the time is in the second preset time T 2 When the gas concentration in the inner culture box 1 is increased or not, delta 1 ＞A 1 Reaches a first preset time T 1 Then further judging that the time is within the second preset time T 2 Whether or not the gas concentration in the inner culture box 1 is increased can prevent the error of gas concentration detection from causing a Δ at a certain moment 1 ＞A 1 The situation of (2) and the misjudgment that causes to make the judgement to the difference between the gas concentration in incubator 1 and the standard concentration more accurate, to the judgement of the switching between main gas supply steel bottle 2 and reserve gas supply steel bottle 3 more accurate.
Illustratively, a first preset time T is set 1 Is 180s, standard concentration C 0 15.0%, first preset value A 1 1.0%, the following description will discuss an embodiment in which the supply of the culture tank 1 from the backup gas cylinder 3 is not switched to.
The first situation is as follows: if the detected gas concentration C in the incubator 1 1 Is 14.0%, the first difference Δ 1 =15.0% -14.0%, namely delta 1 ＝1.0％，△ 1 ＝A 1 At this time, the supply of gas from the spare gas cylinder 3 to the incubator 1 is not switched.
Case two: if the detected gas concentration C in the incubator 1 1 14.5%, the first difference Δ 1 =15.0% -14.5%, Δ 1 ＝0.5％，△ 1 ＜A 1 At this time, the supply of gas from the spare gas cylinder 3 to the incubator 1 is not switched.
Case three: if the detected gas concentration C in the incubator 1 1 13.0%, the first difference Δ 1 =15.0% -13.0%, Δ 1 ＝2.0％，△ 1 ＞A 1 But delta 1 ＞A 1 The duration of time (2) is only 150 seconds, and the supply of gas from the spare gas cylinder 3 to the incubator 1 is not switched to this time.
Illustratively, a first preset time T is set 1 At a standard concentration of C of 180s 0 15.0%, first preset value A 1 1.0%, if the gas concentration C in the incubator 1 is detected 1 13.0%, the first difference Δ 1 =15.0% -13.0%, namely delta 1 ＝2.0％，△ 1 ＞A 1 And Δ 1 ＞A 1 Is 180s, further judging that the time is within a second preset time T 2 Whether the gas concentration in the inner culture box 1 is increased.
Preferably, the judgment is carried out for a second preset time T 2 The specific steps of whether the gas concentration in the inner culture box 1 is increased or not "include:
calculating a second difference Δ 2 ＝C i+n -C i ；
The second difference Delta 2 With a second predetermined value A 2 Comparing;
if Δ 2 ＞A 2 Then, it is determined as being at the second preset time T 2 The concentration of the gas in the inner incubator 1 increases;
wherein, C i The gas concentration, C, in the incubator 1 obtained at the beginning of the judgment i+n The gas concentration in the incubator 1 is obtained every n set times after the start of judgment.
By setting by calculating at a second preset time T 2 Within the two acquisitions of the difference Δ in gas concentration in the incubator 1 2 Whether the value is greater than a second preset value A 2 Whether the gas concentration in the incubator 1 is increased or not is judged, and compared with the method of directly comparing the gas concentrations in the incubators 1 obtained twice, misjudgment caused by detection errors of the gas concentration in the incubator 1 can be avoided, so that the judgment result is more reliable.
In practical applications, those skilled in the art will judge the incubator 1When the gas concentration in the incubator 1 is increased, the gas concentration in the incubator 1 (i.e., C) can be obtained once at the beginning of the judgment i ) The gas concentration in the incubator 1 (i.e., C) is acquired every n set times as a reference concentration for determining whether the gas concentration is increased or not i+n ) Mixing C with i+n And C i Making a difference for judgment; alternatively, the gas concentration in the incubator 1 may be continuously obtained throughout the control, and in this case, the initially obtained gas concentration in the incubator 1 (i.e., C) will be judged i ) As a judgment whether or not the gas concentration is increased by the reference concentration, the gas concentration in the incubator 1 (i.e., C) is obtained i+n ) And C i Such variations and modifications can be made without departing from the spirit and scope of the invention, which should be limited only by the appended claims. Of course, it is preferable to continuously obtain the gas concentration in the incubator 1 throughout the control process.
Illustratively, a first preset time T is set 1 Is 180s, a second preset time T 2 120s, standard concentration of 15.0%, setting time of 10s, first preset value A1 of 1.0%, second preset value A 2 Is 0.2%.
When delta is 1 When the duration of > 15.0% reached 180s, the gas concentration in the incubator 1 detected at that time was designated as C i Assumed to be C i =13.0%, and the gas concentration in the incubator 1 obtained at the interval of 10s is C i+1 =13.5%, second difference Δ 2 ＝C i+1 -C i =13.5% -13.0% =0.5% > 0.2%, indicating that the gas concentration in the incubator 1 is increased within 120s.
If C is present i+1 =13.0%, i.e., Δ 2 =0% < 0.2%; the calculation of the gas concentration C in the incubator 1 obtained at the time of the re-interval of 10s is continued i+2 And C i Difference Δ of 2 Up to delta 2 ＞0.2％。
However, if the gas concentration and C in the incubator 1 were taken at 120s i Difference between delta 2 Are not more than 0.2%, indicating that the gas concentration in the incubator 1 is not increased within 120s。
Preferably, the specific step of "selectively switching to supply gas from the spare gas supply cylinder 3 to the incubator 1 according to the judgment result" includes:
if the judgment result is 'yes', the gas supply of the incubator 1 by the spare gas supply steel cylinder 3 is not switched;
if the judgment result is 'no', the gas supply amount of the main gas supply steel cylinder 2 is increased, and the third preset time T is further judged 3 Whether the gas concentration in the inner incubator 1 is increased;
based on the determination result, the supply of gas from the spare gas cylinder 3 to the incubator 1 is selectively switched.
By such an arrangement, on the one hand, if at the second preset time T 2 Within, the gas concentration in incubator 1 risees to some extent, then explains that main gas supply steel bottle 2 is still carrying out the gas feed to incubator 1, promptly, still has the residual gas in main gas supply steel bottle 2, consequently, does not switch to and carries out the gas feed to incubator 1 by reserve gas supply steel bottle 3 this moment, avoids the residual gas waste in main gas supply steel bottle 2.
On the other hand, if at the second preset time T 2 However, if the gas concentration in the incubator 1 is not increased, it may be caused by the fact that the main gas supply cylinder 2 is empty, or, although there is still residual gas in the main gas supply cylinder 2, the gas is slowly supplied due to insufficient pressure in the main gas supply cylinder 2, and the second predetermined time T is set 2 In the incubator 1, the gas concentration is not increased, and therefore, it is necessary to further increase the amount of gas supplied to the main gas cylinder 2 and detect that the gas concentration is increased for the third preset time T 3 Whether the gas concentration in the inner incubator 1 rises or not is judged to judge whether the main gas supply steel cylinder 2 can supply gas for the incubator 1 or not, and further whether residual gas exists in the main gas supply steel cylinder 2 or not is judged, so that waste of residual gas remaining in the main gas supply steel cylinder 2 can be avoided.
It should be noted that, in practical applications, a person skilled in the art may set the increasing of the air supply amount of the main air supply cylinder 2 to adjust the valve opening of the main air supply cylinder 2 to the maximum opening, or may adjust the opening of the first control valve 211 to be larger, or may intervene in PID control of the main air supply cylinder 2 during normal air supply, such as changing the duty ratio of the air supply that changes during normal air supply to a fixed duty ratio, and so on, and such adjustment and change of the specific implementation manner of increasing the air supply amount of the main air supply cylinder 2 do not depart from the principle and scope of the present invention, and are all included in the protection scope of the present invention.
Preferably, the control method of the present invention further comprises: the increase in the amount of gas supplied to the main gas cylinder 2 is set so that the valve opening of the main gas cylinder 2 is adjusted to the maximum opening.
It should be noted that, in practical applications, the second preset time T can be set by those skilled in the art 2 And a third preset time T 3 Set to be the same, or, the second preset time T can be set 2 And a third preset time T 3 Different arrangements, etc., such that they can be flexibly adjusted and changed without departing from the spirit and scope of the present invention, are intended to be included within the scope of the present invention.
Preferably, the second preset time T 2 And a third preset time T 3 The settings are the same.
Exemplarily, the second preset time T is set 2 And a third preset time T 3 Are set to 120s.
Preferably, the specific step of "selectively switching to supply gas from the spare gas supply cylinder 3 to the incubator 1 according to the judgment result" includes:
if the judgment result is yes, the culture box 1 is not switched to be supplied with gas by the spare gas supply steel cylinder 3;
if the judgment result is 'no', the gas supply to the incubator 1 by the spare gas supply cylinder 3 is switched.
With such an arrangement, on one hand, if the gas concentration in the incubator 1 rises within a third preset time after the gas supply amount of the main gas supply cylinder 2 is increased, which indicates that there is residual gas in the main gas supply cylinder 2, the gas supply to the incubator 1 by the spare gas supply cylinder 3 is not switched, so that the waste of residual gas in the main gas supply cylinder 2 is avoided; on the other hand, if the gas concentration in the incubator 1 does not rise for the third predetermined time after the amount of gas supplied from the main gas cylinder 2 is increased, it means that there is no residual gas in the main gas cylinder 2, and at this time, it is necessary to switch to the supply of gas from the backup gas cylinder 3 to the incubator 1, thereby avoiding the influence of the shortage of the gas supply to the incubator 1 on the growth of the cells and tissues in the incubator 1.
It should be noted that the judgment is made at the third preset time T 3 The specific steps of the inner culture box 1 for determining whether the gas concentration is increased or not and the judgment are carried out in the second preset time T 2 The specific steps of whether the gas concentration in the incubator 1 is increased or not are the same, and therefore, the detailed description thereof is omitted.
It should be noted that, in practical applications, those skilled in the art may set the switching between the supply of the gas from the main gas cylinder 2 to the spare gas cylinder 3 to the supply of the gas to the incubator 1 by the manual operation of the first control valve 211 and the second control valve 311 by the user, or may set the first control valve 211 and the second control valve 311 as electric control valves, and the switching between the supply of the gas from the main gas cylinder 2 to the spare gas cylinder 3 to the supply of the gas to the incubator 1 as automatic switching, and so on, and such flexible adjustment and change should not deviate from the principle and scope of the present invention, and all should be included in the protection scope of the present invention.
Preferably, the gas supply system of the present invention further comprises an alarm device.
It should be noted that, in practical applications, those skilled in the art may set the alarm device on the incubator 1, or may set the alarm device on the main gas supply cylinder 2, or may set the alarm device on the spare gas supply cylinder 3, etc., and such adjustment and change of the specific setting position of the alarm device do not depart from the principle and scope of the present invention, and are all included in the protection scope of the present invention.
Preferably, as shown in fig. 1, the alarm device 12 is provided on the incubator 1.
It should be noted that, in practical applications, a person skilled in the art may configure the alarm device 12 as an audible alarm module, or may configure the alarm device 12 as an optical alarm module, or may configure the alarm device 12 as an acousto-optic combined alarm module, etc., and such adjustments and changes to the specific configuration type of the alarm device 12 are included in the protection scope of the present invention without departing from the principle and scope of the present invention.
Preferably, the alarm device 12 is provided as a combined sound and light alarm module.
Preferably, at a second preset time T 2 In the case where the gas concentration in the incubator 1 is not increased, the control method of the present invention further includes: the alarm device 12 is activated.
With such an arrangement, on the one hand, the alarm device 12 can give an alarm of low concentration in the incubator 1, and can notify the user that the gas concentration in the incubator 1 is lower than the standard concentration, thereby enabling the user to grasp the gas concentration in the incubator 1; on the other hand, the user can be reminded that the amount of gas in the main gas cylinder 2 is not large, and that the supply of gas to the incubator 1 by the backup gas cylinder 3 is to be switched.
It should be noted that, in practical applications, the alarm device 12 is not limited to be set to the second preset time T 2 In the case where the gas concentration in the incubator 1 is not increased, for example, the alarm device 12 may be set to increase the amount of gas supplied from the main gas cylinder 2 and then set to a third preset time T 3 The flexible adjustment and change of the gas concentration in the incubator 1 without increasing the gas concentration, etc. without departing from the principle and scope of the present invention should be included in the protection scope of the present invention. It is of course preferred that at a second preset time T 2 In the case where the concentration of the gas in the incubator 1 is not increased, the alarm device 12 is activated.
Preferably, the control method of the present invention further comprises: after the alarm device 12 is activated, the alarm device 12 stops operating when the supply of gas from the spare gas cylinder 3 to the incubator 1 is switched.
Through the arrangement, the user can be reminded that the switching of the gas supply system is completed at the moment, on one hand, the gas concentration in the incubator 1 can be gradually recovered to the normal concentration, so that the user can master the gas concentration condition in the incubator 1; on the other hand, the user can be reminded to replace the exhausted main gas supply cylinder 2 with a cylinder filled with gas, so that when the gas in the backup gas supply cylinder 3 is exhausted, the main gas supply cylinder 2 is switched to supply gas to the incubator 1.
Preferably, as shown in fig. 3, the control method of the present invention further includes:
s233: if Δ 1 ＞A 1 Reaches a fourth preset time T 4 In this case, the supply of gas from the incubator 1 is switched to the standby gas supply cylinder 3.
By such setting, if Δ 1 ＞A 1 Reaches a fourth preset time T 4 When the gas concentration in the incubator 1 is lower than the standard concentration for a long time, the gas supply from the spare gas supply cylinder 3 to the incubator 1 is switched in time, so that the influence of the gas concentration in the incubator 1 lower than the standard concentration for a long time on the normal growth of cells and tissues in the incubator 1 can be avoided.
It is noted that the air supply system of the present invention includes a controller configured to be able to execute the control method described above.
It should be noted that, in practical applications, a person skilled in the art may set the controller to be in communication connection with the gas concentration sensor 11, the first control valve 211, the second control valve 311, and the alarm device 12, so as to realize intelligent collection of the gas concentration in the incubator 1, intelligent switching of the gas supply system, and intelligent reminding of the conditions in the incubator 1.
It should be noted that although the embodiment of the present invention has been described with reference to supplying gas to the incubator 1 from the main gas cylinder 2 first and switching to supplying gas to the incubator 1 from the auxiliary gas cylinder 3 when the gas in the main gas cylinder 2 is used up, the control method of the present invention is also applicable to the case where the gas in the auxiliary gas cylinder 3 is used up when the gas in the auxiliary gas cylinder 3 is used up and switching to supplying gas to the incubator 1 from the main gas cylinder 2 when the gas in the auxiliary gas cylinder 3 is used up, and the like, and such flexible adjustment and change are not departing from the principle and scope of the present invention and are included in the protection scope of the present invention.
So far, the technical solutions of the present invention have been described in connection with the preferred embodiments shown in the drawings, but it is apparent to those skilled in the art that the scope of the present invention is not limited to these specific embodiments. Equivalent changes or substitutions of related technical features can be made by those skilled in the art without departing from the principle of the invention, and the technical scheme after the changes or substitutions can fall into the protection scope of the invention.
Claims (10)
1. A control method of a gas supply system is characterized in that the gas supply system comprises an incubator, a main gas supply steel cylinder and a standby gas supply steel cylinder, and the control method comprises the following steps:
obtaining the gas concentration C in the incubator 1 ；
According to the gas concentration C 1 And standard concentration C 0 And selectively switching to supply gas to the incubator by the spare gas supply steel cylinder.
2. The control method according to claim 1, wherein "in accordance with the gas concentration C 1 And standard concentration C 0 The specific steps of selectively switching to the supply of gas from the spare gas supply cylinder to the incubator comprise:
calculating a first difference Δ 1 ＝C 0 -C 1 ；
The first difference Delta 1 With a first predetermined value A 1 Comparing;
and selectively switching to supply gas to the incubator from the standby gas supply steel cylinder according to the comparison result.
3. The control method according to claim 2, wherein the step of selectively switching to supply the gas from the backup gas cylinder to the incubator according to the comparison result comprises:
if a is equal 1 ≤A 1 If the culture box is not switched to the spare gas supply steel cylinder, the culture box is not supplied with gas;
if Δ 1 ＞A 1 Reaches a first preset time T 1 Then further determine the time at the second preset time T 2 Whether the concentration of gas in the incubator is elevated;
and selectively switching to supply gas to the incubator by the spare gas supply steel cylinder according to the judgment result.
4. The control method according to claim 3, wherein the step of selectively switching to supply gas from the backup gas supply cylinder to the incubator according to the determination result comprises:
if the judgment result is yes, the spare gas supply steel cylinder is not switched to supply gas to the incubator;
if the judgment result is 'no', the gas supply amount of the main gas supply steel cylinder is increased, and further judgment is carried out for a third preset time T 3 Whether the concentration of gas in the incubator is elevated;
and selectively switching to the spare gas supply steel cylinder to supply gas to the incubator according to the judgment result.
5. The control method according to claim 4, wherein the step of selectively switching to supply gas from the backup gas supply cylinder to the incubator according to the determination result comprises:
if the judgment result is yes, the spare gas supply steel cylinder is not switched to supply gas to the incubator;
if the judgment result is 'no', the spare gas supply steel cylinder is switched to supply gas to the incubator.
6. The control method according to claim 4, wherein the gas supply system further includes an alarm device, and in the case where the determination result is "no", the control method further includes:
and starting the alarm device.
7. The control method according to claim 4, wherein the step of increasing the amount of gas supplied to the main gas cylinder comprises:
and adjusting the opening degree of the valve of the main gas supply steel cylinder to the maximum opening degree.
8. The control method according to claim 3, characterized in that the control method of the invention further comprises:
if Δ 1 ＞A 1 Reaches a fourth preset time T 4 And when the culture box is used, the culture box is switched to be supplied with gas by the standby gas supply steel cylinder.
9. The control method according to any one of claims 3 to 8, wherein "the judgment is made at the second preset time T 2 The specific steps of whether the gas concentration in the incubator is increased or not "include:
calculating a second difference Δ 2 ＝C i+n -C i ；
The second difference Delta 2 And a second preset value A 2 Carrying out comparison;
if Δ 2 ＞A 2 If yes, the judgment is made that the time is within the second preset time T 2 Whereby the concentration of gas in said incubator increases;
wherein, C i The gas concentration, C, in the incubator obtained at the beginning of the judgment i+n The gas concentration in the incubator obtained at every n set times after the start of judgment.
10. An air supply system comprising a controller configured to be able to perform the control method of any one of claims 1 to 9.
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