CN115124376A

CN115124376A - Compound biological fertilizer and preparation method thereof

Info

Publication number: CN115124376A
Application number: CN202210821815.0A
Authority: CN
Inventors: 张爱中; 姜俊玲; 张东方; 周春晖; 高松
Original assignee: Henan Zhongwei Chunyu Plant Nutrition Co ltd
Current assignee: Henan Zhongwei Chunyu Plant Nutrition Co ltd
Priority date: 2022-07-13
Filing date: 2022-07-13
Publication date: 2022-09-30

Abstract

The invention belongs to the technical field of biological fertilizers, and particularly discloses a compound biological fertilizer and a preparation method thereof, wherein the compound biological fertilizer comprises the following components in parts by weight: 30-48 parts of organic fermentation product, 60-100 parts of inorganic fermentation product, 5-10 parts of composite microbial bacteria, 20-40 parts of composite nutritive salt, 10-20 parts of humic acid, 10-20 parts of amino acid and 5-15 parts of amino acid chelating agent. Compared with the prior art, the compound biological fertilizer provided by the invention can effectively promote crops to absorb nutrients, increase the content of the nutrients of the crops, has the multifunctional advantages of root promotion, bacteriostasis, yield increase, environmental protection and the like, and effectively meets the requirement of agricultural production on the diversification of the functions of the fertilizer.

Description

Compound biological fertilizer and preparation method thereof

Technical Field

The invention belongs to the technical field of biological fertilizers, and particularly relates to a compound biological fertilizer and a preparation method thereof.

Background

In the existing agricultural production, chemical fertilizers are adopted to cultivate crops in large quantity in order to improve the yield of the crops. However, the dependence of crops on chemical fertilizers is increased year by year due to the long-term use of a large amount of chemical fertilizers, the land fertilizers are less and less, the actual utilization rate is only 35% when the crops are irrigated by using the chemical fertilizers, the rest of the chemical fertilizers are volatilized into the atmosphere or flow into rivers and lakes along with water flow, so that the environmental problems of soil hardening, atmosphere pollution, water source pollution and the like are serious day by day, and the chemical fertilizers pose serious threats to the health and survival of human beings and other organisms. Moreover, the utilization rate of chemical fertilizers is decreasing year by year, the main reason is that organic matters in soil are continuously reduced, beneficial microorganisms capable of stimulating the growth of crops and improving the disease resistance of crops are greatly reduced, and in order to change the current situation, organic matters and beneficial microorganisms need to be added into the soil, so that the biological fertilizers are produced at the same time.

Biofertilizers are a special group of fertilizers containing a large number of living microorganisms which, when applied to the soil, are able to actively move under suitable conditions: some of the plants can propagate in large quantities around the roots of the crops to play a role of self-generation nitrogen fixation or combined nitrogen fixation; some can also decompose mineral elements of phosphorus and potassium to supply crops to absorb or secrete growth hormone to stimulate the growth of the crops. It follows that biofertilizers do not directly supply nutrients required by crops, but stimulate the growth of crops by providing nutrients required by crops and producing hormones through the active action of a large number of living microorganisms in soil, which is fundamentally different from the action of other organic fertilizers and fertilizers.

However, the existing biological fertilizer has single function, and can not meet the requirement of agricultural production on the diversification of the fertilizer function.

Disclosure of Invention

The invention aims to: aiming at the defect of single function of the existing biological fertilizer, the multifunctional compound biological fertilizer is provided, can effectively promote crops to absorb nutrients, increases the content of the nutrients of the crops, and has the multifunctional advantages of root promotion, bacteriostasis, yield increase, environmental protection and the like.

In order to achieve the purpose, the invention adopts the following technical scheme:

a compound biofertilizer comprises the following components in parts by weight:

30-48 parts of organic fermentation product, 60-100 parts of inorganic fermentation product, 5-10 parts of compound microorganism bacterium, 20-40 parts of compound nutrient salt, 10-20 parts of humic acid, 10-20 parts of amino acid and 5-15 parts of amino acid chelating agent;

the organic fermentation product is prepared by mixing chicken manure, cow manure and pig manure according to the mass ratio of 1: 1-2; the inorganic fermentation product is prepared by mixing corn straws, wheat bran, rape stalks and rice husks according to the mass ratio of 1: 2-3: 1-2: 1;

the composite nutrient salt is prepared by mixing monopotassium phosphate, magnesium sulfate, calcium carbonate, magnesium sulfate and zinc sulfate according to the mass ratio of 1-2: 1:1:1: 1;

the amino acid chelating agent is prepared by mixing amino acid chelated iron, amino acid chelated copper, amino acid chelated calcium, amino acid chelated magnesium and amino acid chelated zinc according to a mass ratio of 1-2: 1:1: 1;

the composite microbial strain is prepared by mixing streptomyces avermitilis, rhodopseudomonas palustris, bacillus megaterium, violaceous violet spore bacteria, pasteurella puncture and bacillus subtilis according to the mass ratio of 1: 1-2: 2-3, and the effective viable count of the streptomyces avermitilis, rhodopseudomonas palustris, bacillus megaterium, violaceous violet spore bacteria, pasteurella puncture and bacillus subtilis is (1-3) multiplied by 10 ⁹ cfu/mL。

Preferably, the color transfer promoter also comprises the following color transfer promoter in parts by weight: 10-20 parts of brown algae oligosaccharide extracting solution, 0.5-1.5 parts of phenylalanine, 0.5-1.5 parts of methionine, 0.5-1.5 parts of arginine, 0.5-1.5 parts of leucine, 0.5-1 part of astacin, 1-3 parts of vitamin C, 1-5 parts of manganese sulfate and 1-5 parts of magnesium sulfate; wherein the content of the brown algae oligosaccharide with the degree of polymerization of 4-8 in the brown algae oligosaccharide extracting solution is more than or equal to 92%, and the solid content is more than or equal to 18%. The auxiliary agent comprises brown algae oligosaccharide extracting solution, phenylalanine, methionine, arginine, leucine, astaxanthin, vitamin C, manganese sulfate and magnesium sulfate in a specific proportion. By adopting the specific components and adjusting the content of the fertilizer biological synergistic additive, the synthesis and accumulation of plant anthocyanin, carotenoid and the like can be promoted, the coloring of fruits can be promoted, the sugar metabolism of plants can be accelerated, the soluble sugar content of the fruits can be increased, and the sugar-acid ratio can be improved; especially, the application effect on crops such as tomatoes, oranges, grapes and the like is outstanding, the fruits are uniformly colored, and the soluble solid content is high.

The raw material of the brown algae oligosaccharide extracting solution is polysaccharide extracted from marine brown algae, and the brown algae oligosaccharide is prepared by directional and accurate degradation, separation and purification. The brown algae oligosaccharide is linearly composed of beta-D-mannuronic acid and alpha-L-guluronic acid, and the polymerization degree is below 20. The polymerization degrees of the brown alginate oligosaccharides prepared by different degradation processes are different, and the brown alginate oligosaccharides with different polymerization degrees have obvious difference on biological activity and functions. The fucoidan extract with the content of the fucoidan with the polymerization degree of 4-8 being more than or equal to 92% and the solid content being more than or equal to 18% is beneficial to improving the color conversion property of plant fruits. The brown algae oligosaccharide extracting solution can promote the growth of crops, stimulate the expression of auxin related genes through the combined action of hormone content, lipase, amylase and protease, promote the synthesis and accumulation of plant anthocyanin and carotenoid, and promote color conversion. The phenylalanine, the methionine, the arginine and the leucine belong to amino acids, the synthesis path of plant anthocyanin is phenylalanine metabolism, and the phenylalanine, the methionine, the arginine and the leucine are used in combination to provide anthocyanin raw materials for crop color transfer to a certain extent and promote color transfer. Wherein, the astaxanthin can rapidly permeate into the plant body, promote the flow of cell protoplasm, improve the cell activity and enhance the photosynthesis. The manganese sulfate and the magnesium sulfate are used as supplement components of trace elements, and form a synergistic effect with other nutrient elements, so that the manganese sulfate and the magnesium sulfate are beneficial to being absorbed by crops, and the growth of the crops is promoted.

Preferably, the sweet-enhancing beverage also comprises a sweet-enhancing additive which consists of the following components in parts by weight: 10-20 parts of seaweed oligosaccharide extracting solution, 1-5 parts of cysteine, 1-5 parts of proline and 1-5 parts of monopotassium phosphate; wherein the content of the algal oligosaccharide with the degree of polymerization of 3-6 in the algal oligosaccharide extracting solution is more than or equal to 92%, and the solid content is more than or equal to 15%. The sweetening aid adopts specific components and adjusts the content of the components, so that the sweetening aid can adjust the growth of plants and promote the conversion of crops from vegetative growth to reproductive growth; the photosynthetic efficiency of the leaves is improved, nutrients required by the fruit in the expansion period are supplemented, the accumulation of dry matters of the fruit is promoted, the sugar-acid ratio and the taste of the fruit are improved, and the yield of the fruit is increased; the fruit sugar-free sweetener has obvious application effect on crops such as apples, oranges, grapes and the like, can effectively improve the accumulation of dry matters and the sugar content of fruits, increases the sweetness and the mouthfeel of the fruits and improves the yield of the fruits.

The seaweed oligosaccharide extracting solution can promote the growth of crops, stimulates the expression of auxin related genes through the combined action of hormone content, lipase, amylase and protease, and promotes the synthesis and accumulation of plant carbohydrate substances. The seaweed oligosaccharide extracting solution with the polymerization degree of 3-6, the content of seaweed oligosaccharide being not less than 92% and the solid content being not less than 15% is beneficial to improving sweetness and taste of plant fruits. Wherein, the cysteine and the proline belong to amino acid, and the amino acid is adopted in combination to be beneficial to the accumulation of crop carbohydrate substances. Wherein, the monopotassium phosphate can promote the growth of crops, improve photosynthesis and facilitate the accumulation of carbohydrate substances.

Preferably, the soil conditioner also comprises the following soil conditioner consisting of the following components in parts by weight: 10-20 parts of phosphogypsum powder, 1-10 parts of sulfur powder, 1-5 parts of soil humic acid, 1-5 parts of fulvic acid and 1-10 parts of calcium lignosulfonate; wherein the particle size of the phosphogypsum powder is 160-180 mu m. The soil conditioner comprises short-term strong efficacy components, long-term slow release components and nutrient root system components. The short-term strong efficacy components comprise sulfur powder, the pH of the soil is reduced by adding the sulfur powder, the alkalinity of the soil is further reduced after sulfur bacteria in the soil decompose sulfur and convert the sulfur into sulfuric acid, and the acid is continuously adjusted. The nutrient root system components comprise calcium lignosulphonate, humic acid and fulvic acid, and the calcium lignosulphonate can combine a large amount of humic acid and fulvic acid, so that the growth of the root system can be promoted, and the nutrient absorption efficiency can be improved; meanwhile, the calcium lignosulfonate is also beneficial to granulation of the fertilizer and promotion of disintegration after water dissolution. The long-term slow-release components comprise phosphogypsum powder, alkaline soil mainly contains sodium carbonate, sodium bicarbonate and other substances, the substances are alkaline due to hydrolysis, the phosphogypsum powder has certain acidity, and the phosphogypsum powder can be used as a slow-release carrier to gradually reduce the pH value and the alkalization degree of the soil, so that the effect of slowly improving the saline-alkaline property of the soil is achieved.

Preferably, the growth promoting additive also comprises the following components in parts by weight: 10-20 parts of chitosan extracting solution, 10-20 parts of seaweed oligosaccharide extracting solution, 1-3 parts of alanine, 1-3 parts of cysteine, 1-3 parts of histidine, 1-3 parts of lysine, 1-3 parts of glycine, 0.5-5 parts of EDTA chelated iron, 1-5 parts of boric acid, 1-5 parts of manganese sulfate, 1-5 parts of magnesium sulfate and 1-5 parts of zinc sulfate; the chitosan extraction solution contains chitosan with 4-8 polymerization degree more than or equal to 92% and solid content more than or equal to 15%, and the alginate-derived oligosaccharide with 3-6 polymerization degree in the alginate-derived oligosaccharide extraction solution contains alginate-derived oligosaccharide with 3-6 polymerization degree more than or equal to 92% and solid content more than or equal to 15%. The growth promoting additive adopts specific components and adjusts the content of the components, so that the damage to heat sensitive components is effectively avoided, the growth of plant root systems can be promoted by applying the growth promoting additive to plants, the nutrient absorption and transportation efficiency of the root systems is improved, and the yellowing phenomenon caused by the loss of plant silver nutrients is prevented; meanwhile, synthesis of plant chlorophyll can be promoted, photosynthetic efficiency of leaves is improved, and fruit yield is effectively improved.

The seaweed oligosaccharide is similar to phytohormone, can stimulate the growth of root systems and stem leaves of crops, and improves the yield of the crops; can induce plants to generate resistance, improve the oxidation resistance of the plants and delay the crop aging. The seaweed oligosaccharide with the polymerization degree of 3-6 has small molecular weight, is not easy to flocculate and precipitate, is easier to be absorbed by plants, and has more remarkable effects of promoting the photosynthesis of the plants and preventing physiological yellowing. The alanine, the cysteine, the histidine, the lysine and the glycine belong to amino acids, and the amino acids are adopted in combination, so that the content of phytoalexin in plants can be improved, and the photosynthesis can be enhanced.

Preferably, the anti-aging agent also comprises the following anti-aging additives in parts by weight: 10-20 parts of brown algae oligosaccharide extracting solution, 61-5 parts of vitamin B and 1-5 parts of anhydrous citric acid; wherein the content of the fucoidan with the degree of polymerization of 4-8 in the fucoidan extracting solution is more than or equal to 92%, and the solid content is more than or equal to 18%. The anti-aging auxiliary agent is prepared by adding brown algae oligosaccharide extracting solution, vitamin B6 and anhydrous citric acid, and adjusting the respective contents of the three components to realize the synergistic effect of the three components, so that the obtained biological synergistic auxiliary agent can effectively promote the root development of crops, improve the anti-aging capability of the crops, prolong the growth period of the crops and improve the yield of the crops when being applied to the plants.

Preferably, the rooting-promoting agent also comprises the following components in parts by weight: 10-15 parts of indolebutyric acid, 10-15 parts of potassium indolebutyrate, 1-5 parts of alpha-naphthylacetic acid and 1-5 parts of alpha-sodium naphthylacetate. The root growth promoting assistant can effectively promote the development of crop roots and improve the survival rate of crops by adopting specific components and adjusting the content of the components.

Preferably, the fiber-chelated phosphate fertilizer additive comprises 1-5 parts by weight of fiber-chelated phosphate fertilizer additive, wherein the fiber-chelated phosphate fertilizer additive comprises monoammonium phosphate and chelated cellulose fibers in a mass ratio of 10-15: 1; the chelate cellulose fiber is obtained by acidifying cellulose fiber with lipoic acid and then carrying out condensation reaction with 2- (diphenylphosphino) ethylamine, wherein the effective phosphorus content of monoammonium phosphate is not less than 60%, and the total nitrogen content is not less than 15%. This application uses monoammonium phosphate and chelate type cellulose base fibre to carry out the chelate fiber phosphorus fertilizer auxiliary agent that the complex was made, and this auxiliary agent not only can reduce soil and to the fixed of phosphorus, has prevented the bad consequence that available state phosphorus turns into invalid state phosphorus in a large number, has improved the utilization of crops to phosphorus, but also has solved the easy defect of caking of monoammonium phosphate itself.

The chelating cellulose fiber is prepared by using agricultural waste as a base material, firstly carrying out acidification degradation to obtain the cellulose fiber, and then sequentially carrying out alkali liquor soaking treatment, lipoic acid acidification treatment and condensation reaction of 2- (diphenylphosphino) ethylamine on the cellulose fiber to finally obtain the chelating cellulose fiber. Cellulose acidified by lipoic acid has good hydrophilicity, and chelate cellulose fibers obtained by condensation reaction with carboxyl on the surface of the cellulose are connected with a large number of diphenylphosphine groups through amide bonds, the diphenylphosphine groups are organic phosphorus compound ligands, can chelate various metal ions, contain phosphorus elements and can be used as organic phosphorus fertilizers after being decomposed in a later period. The chelating cellulose fiber enhances the utilization of phosphate fertilizer from two aspects, and the first is that the chelating cellulose fiber has better adsorbability to metal ions and can solidify the metal ions (calcium, aluminum, iron and the like) for solidifying phosphorus on the fiber in one step, so that the solidification of the metal to the phosphorus is weakened, and the invalid state conversion of the phosphorus is reduced; secondly, the phosphorus element has strong wettability, the phosphorus element moves in the soil by diffusion, and one reason of low utilization rate of the phosphate fertilizer is that the mobility of the phosphorus element in the soil is poor, that is, the diffusion coefficient of the phosphorus element is small, the moving distance within 24 hours is only 1-4mm, and the strong wettability can enable the moisture outside the cellulose fiber to enter the internal environment more smoothly, so that the monoammonium phosphate compounded with the cellulose fiber can be rapidly diffused into the soil by the moisture and absorbed by plants.

Preferably, the slow-release charring material also comprises a slow-release charring material, wherein the slow-release charring material is prepared by charring 10-12 parts by weight of fennel, 5-10 parts by weight of pokeberry root, 5-8 parts by weight of golden cypress and 5-10 parts by weight of pinellia ternate at 500-800 ℃ for 2.0-3.0 h. The slow release carbide formed by high temperature carbonization has a microporous structure, and the microporous structure can load other raw materials, so that the prepared biological fertilizer has a slow release effect.

In addition, the invention also provides a preparation method of the compound biological fertilizer, which comprises the following steps:

1) weighing the raw materials in parts by weight;

2) mixing organic fermentation products, inorganic fermentation products, humic acid and compound nutrient salt, feeding the mixture into a crusher for crushing, and feeding the crushed material into a fermentation tank;

3) adding a formula amount of compound microorganism bacteria and a proper amount of water into a fermentation tank, uniformly stirring, fermenting and decomposing, wherein the fermentation temperature is 20-60 ℃, fermenting for 25-35 days, and naturally airing after fermentation is finished to obtain a biological fertilizer mixture;

4) and adding the biological fertilizer mixture, the amino acid and the amino acid chelating agent into a stirrer, uniformly mixing, crushing and screening into powder to obtain the compound biological fertilizer.

The invention has the beneficial effects that: the invention relates to a compound biofertilizer which comprises the following components in parts by weight: 30-48 parts of organic fermentation product, 60-100 parts of inorganic fermentation product, 5-10 parts of composite microbial bacteria, 20-40 parts of composite nutrient salt, 10-20 parts of humic acid, 10-20 parts of amino acid and 5-15 parts of amino acid chelating agent. Compared with the prior art, the method has the following effective effects:

1) the amino acid is added to serve as a fertilizer and then is absorbed more easily, the amino acid and an amino acid chelating agent are added to the biological fertilizer, the amino acid has the effect of complexing metal ions, trace elements required by plants are carried into the plants easily, the utilization rate of the plants on various nutrients is improved, the amino acid plays an important role in plant metabolism, the volatilization loss of nitrogen and the fixation of phosphorus can be reduced, the amino acid chelating agent is prepared by chelating the amino acid and slightly soluble trace elements, the stable effect and the long effect of the trace elements are ensured, the nutrient absorption of crops can be promoted after the fertilizer is applied, the content of the crop nutrients is increased, and the high-yield and high-quality effects are achieved.

2) The organic fertilizer is prepared from inorganic fermentation materials formed by synergistic combination of corn straws, wheat bran, rape stems and rice husks and organic fermentation materials formed by synergistic combination of chicken manure, cow manure and pig manure.

3) The compound nutrient salt formed by synergistic combination of monopotassium phosphate, magnesium sulfate, calcium carbonate, magnesium sulfate and zinc sulfate can be used as a supplement component of trace elements, and can form synergistic action with other nutrient elements, thereby being beneficial to absorption of crops and promoting growth of the crops.

4) After entering soil, the compound microorganism bacterium formed by mixing streptomyces avermitilis, rhodopseudomonas palustris, bacillus megaterium, violaceous violet spore bacterium, pasteurella puncture and bacillus subtilis forms a symbiotic multiplication relationship with microorganisms in the soil, so that the growth of harmful bacteria is inhibited and the harmful bacteria are converted into beneficial bacteria, and the microorganisms can generate a large amount of metabolites in the growth and reproduction process, promote the decomposition and conversion of organic matters, effectively promote the growth of crops and obviously improve the yield of the crops; and the microbial bacteria have small mutual interference, obvious fertilizer effect and quick action effect, and have various functions of fixing nitrogen, dissolving phosphorus, dissolving potassium, killing insects and inhibiting bacteria. Wherein, the bacillus megaterium can decompose soil lecithin 62%, decompose nucleic acid 56%, decompose organic compounds and release phosphorus 91%. The phosphorus and potassium fixed in the soil can be converted into quick-acting phosphorus and quick-acting potassium which can be absorbed and utilized by plants, so that the deficiency of phosphorus and potassium nutrients of crops can be supplemented; wherein, the rhodopseudomonas palustris has nitrogen fixation capacity and improves the nitrogen level of soil; promoting soil substance transformation, improving soil structure, and improving soil fertility; harmful substances in the soil are eliminated, and the conversion of the pollutants is promoted; the physiological active substances such as proline, vitamins, coenzyme Q and the like are generated, cells are activated, the root development is promoted, and the photosynthesis and the growth capacity are improved; the pathogenic force of pathogenic substances is passivated, beneficial microorganisms such as actinomycetes and the like in soil are activated, pathogenic bacteria such as filamentous fungi and the like are inhibited, and the disease resistance and disease prevention capability of crops are enhanced. The streptomyces avermitilis can produce avermectin, and can kill bollworm, corn borer, liriomyza sativae and other overground and root-knot nematode, cutworm, wild skullus, wireworm and other pests effectively and reduce the dosage of chemical pesticide.

Detailed Description

In order to make the technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides a compound biofertilizer which comprises the following components in parts by weight:

the compound nutrient salt is prepared by mixing monopotassium phosphate, magnesium sulfate, calcium carbonate, magnesium sulfate and zinc sulfate according to the mass ratio of 1-2: 1:1:1: 1;

Wherein, all the microbial bacteria can be obtained by market, manufacturers do not make specific limitation, and the effective viable count meets the requirements.

Compared with the prior art, the fertilizer is easier to absorb after amino acid is added as the fertilizer, the amino acid and the amino acid chelating agent are added into the biological fertilizer, the amino acid has the effect of complexing metal ions, trace elements required by plants are easy to carry into the plants, the utilization rate of the plants on various nutrients is improved, the amino acid plays an important role in plant metabolism and can reduce the volatilization of nitrogen and the fixation of phosphorus, the amino acid chelating agent is prepared by carrying out a chelating reaction on the amino acid and slightly soluble trace elements, the stable effect and the long effect of the trace elements are ensured, the crops can be promoted to absorb the nutrients after the fertilizer is applied, the content of the nutrients of the crops is increased, and the effects of high yield and high yield are achieved. The organic fertilizer is prepared from inorganic fermentation materials formed by synergistic combination of corn straws, wheat bran, rape stems and rice husks and organic fermentation materials formed by synergistic combination of chicken manure, cow manure and pig manure. The compound nutrient salt formed by synergistic combination of monopotassium phosphate, magnesium sulfate, calcium carbonate, magnesium sulfate and zinc sulfate can be used as a supplement component of trace elements, and can form synergistic action with other nutrient elements, thereby being beneficial to absorption of crops and promoting growth of the crops. After entering soil, the compound microorganism bacterium formed by mixing streptomyces avermitilis, rhodopseudomonas palustris, bacillus megaterium, viola lilacinus, pasteurella puncture and bacillus subtilis forms a symbiotic proliferation relationship with microorganisms in the soil, so that harmful bacteria are inhibited from growing and are converted into beneficial bacteria, the microorganisms can generate a large amount of metabolites in the growth and propagation processes, decomposition and conversion of organic matters are promoted, the growth of crops can be effectively promoted, and the yield of the crops is remarkably improved; and the microbial bacteria have small mutual interference, obvious fertilizer effect and quick action effect, and have various functions of fixing nitrogen, dissolving phosphorus and potassium, killing pests and inhibiting bacteria. Wherein, the bacillus megaterium can decompose 62 percent of soil lecithin, 56 percent of nucleic acid and organic compounds and release 91 percent of phosphorus. Can convert the phosphorus and the potassium fixed in the soil into the quick-acting phosphorus and the quick-acting potassium which can be absorbed and utilized by plants, and can supplement the deficiency of phosphorus and potassium nutrients of crops. Wherein, the rhodopseudomonas palustris has nitrogen fixation capacity and improves the nitrogen level of soil; promoting soil substance transformation, improving soil structure, and improving soil fertility; harmful substances in the soil are eliminated, and the conversion of the pollutants is promoted; the physiological active substances such as proline, vitamins, coenzyme Q and the like are generated, cells are activated, the root development is promoted, and the photosynthesis and the growth capacity are improved; the pathogenic force of pathogenic substances is passivated, beneficial microorganisms such as actinomycetes and the like in soil are activated, pathogenic bacteria such as filamentous fungi and the like are inhibited, and the disease resistance and disease prevention capability of crops are enhanced. The streptomyces avermitilis can produce avermectin, and can kill bollworm, corn borer, liriomyza sativae and other overground and root-knot nematode, cutworm, wild skullus, wireworm and other pests effectively and reduce the dosage of chemical pesticide.

Further, the color transfer promoter comprises the following color transfer promoter in parts by weight: 10-20 parts of brown algae oligosaccharide extracting solution, 0.5-1.5 parts of phenylalanine, 0.5-1.5 parts of methionine, 0.5-1.5 parts of arginine, 0.5-1.5 parts of leucine, 0.5-1 part of astacin, 1-3 parts of vitamin C, 1-5 parts of manganese sulfate and 1-5 parts of magnesium sulfate; wherein the content of the fucoidan with the degree of polymerization of 4-8 in the fucoidan extracting solution is more than or equal to 92%, and the solid content is more than or equal to 18%.

The preparation method of the color transfer promoter comprises the following steps:

1) mixing the brown algae oligosaccharide extracting solution, phenylalanine, methionine, arginine, leucine, astaxanthin, vitamin C, manganese sulfate and magnesium sulfate in proportion, and adjusting the pH value to 3-4 to obtain a mixed solution;

2) and (3) uniformly mixing the mixed solution for 10-30 min by using a dispersing emulsifying machine, and finally preparing the mixed solution into micro-particles by using a droplet pressure spray drying tower, wherein the inlet temperature of the drying tower is 145-155 ℃, and the outlet temperature of the drying tower is 70-80 ℃, so that the color-transfer-promoting additive is obtained.

The auxiliary agent comprises brown algae oligosaccharide extracting solution, phenylalanine, methionine, arginine, leucine, astaxanthin, vitamin C, manganese sulfate and magnesium sulfate in a specific proportion. According to the fertilizer biological synergistic additive, the specific components are adopted, the content of the components is adjusted, the synthesis and accumulation of plant anthocyanin, carotenoid and the like can be promoted, the coloring of fruits is promoted, the plant glycometabolism is accelerated, the soluble sugar content of the fruits is increased, and the sugar-acid ratio is improved; especially, the application effect on crops such as tomatoes, oranges, grapes and the like is outstanding, the fruits are uniformly colored, and the soluble solid content is high.

The raw material of the brown algae oligosaccharide extracting solution is polysaccharide extracted from marine brown algae, and the brown algae oligosaccharide is prepared by directional and accurate degradation, separation and purification. The brown algae oligosaccharide is linearly composed of beta-D-mannuronic acid and alpha-L-guluronic acid, and the polymerization degree is below 20. The polymerization degrees of the brown algae oligosaccharides prepared by different degradation processes are different, and the brown algae oligosaccharides with different polymerization degrees also have obvious difference on biological activity and functions. The brown alginate oligosaccharide extract with the polymerization degree of 4-8, the content of the brown alginate oligosaccharide being more than or equal to 92% and the solid content being more than or equal to 18%, is beneficial to improving the color conversion performance of plant fruits. The brown algae oligosaccharide extracting solution can promote the growth of crops, stimulate the expression of auxin related genes through the combined action of hormone content, lipase, amylase and protease, promote the synthesis and accumulation of plant anthocyanin and carotenoid, and promote color conversion. The phenylalanine, the methionine, the arginine and the leucine belong to amino acids, the synthesis path of plant anthocyanin is phenylalanine metabolism, and the phenylalanine, the methionine, the arginine and the leucine are used in combination to provide anthocyanin raw materials for crop color transfer to a certain extent and promote color transfer. Wherein, the astaxanthin can rapidly permeate into the plant body, promote the flow of cell protoplasm, improve the cell activity and enhance the photosynthesis. The manganese sulfate and the magnesium sulfate are used as supplement components of trace elements, and form a synergistic effect with other nutrient elements, so that the manganese sulfate and the magnesium sulfate are beneficial to being absorbed by crops, and the growth of the crops is promoted.

Further, the sweet-enhancing additive comprises the following components in parts by weight: 10-20 parts of seaweed oligosaccharide extracting solution, 1-5 parts of cysteine, 1-5 parts of proline and 1-5 parts of monopotassium phosphate; wherein the content of the algal oligosaccharide with the degree of polymerization of 3-6 in the algal oligosaccharide extracting solution is more than or equal to 92%, and the solid content is more than or equal to 15%.

The preparation method of the sweetening aid comprises the following steps:

1) mixing the seaweed oligosaccharide extracting solution, cysteine, proline and potassium dihydrogen phosphate in proportion, and adjusting the pH value to 3-4 to obtain a mixed solution;

2) and (3) uniformly mixing the mixed solution for 10-30 min by using a dispersing emulsifying machine, and finally preparing the mixed solution into micro-particles by using a droplet pressure spray drying tower, wherein the inlet temperature of the drying tower is 145-155 ℃, and the outlet temperature of the drying tower is 80-90 ℃, so that the sweetening aid is obtained.

The sweetening aid adopts specific components and adjusts the content of the components, so that the sweetening aid can adjust the growth of plants and promote the conversion of crops from vegetative growth to reproductive growth; the photosynthetic efficiency of the leaves is improved, nutrients required by the fruit in the expansion period are supplemented, the accumulation of dry matters of the fruit is promoted, the sugar-acid ratio and the taste of the fruit are improved, and the yield of the fruit is increased; the fruit sugar-free sweetener has obvious application effect on crops such as apples, oranges, grapes and the like, can effectively improve the accumulation of dry matters and the sugar content of fruits, increases the sweetness and the mouthfeel of the fruits and improves the yield of the fruits.

The seaweed oligosaccharide extracting solution can promote the growth of crops, stimulates the expression of auxin related genes through the combined action of hormone content promotion, lipase, amylase and protease, and promotes the synthesis and accumulation of plant saccharides. According to the application, the content of the algal oligosaccharide with the polymerization degree of 3-6 is more than or equal to 92%, and the solid content of the algal oligosaccharide extracting solution is more than or equal to 15%, so that the sweetness and the mouthfeel of plant fruits can be improved. Wherein, the cysteine and the proline belong to amino acid, and the amino acid is adopted in combination to be beneficial to the accumulation of crop carbohydrate substances. Wherein, the monopotassium phosphate can promote the growth of crops, improve photosynthesis and facilitate the accumulation of carbohydrate substances.

Further, the soil conditioner comprises the following soil conditioner in parts by weight: 10-20 parts of phosphogypsum powder, 1-10 parts of sulfur powder, 1-5 parts of soil humic acid, 1-5 parts of fulvic acid and 1-10 parts of calcium lignosulfonate; wherein the particle size of the phosphogypsum powder is 160-180 mu m.

The preparation method of the soil conditioner comprises the following steps:

1) weighing phosphogypsum powder, sulfur powder, soil humic acid, fulvic acid and calcium lignosulfonate according to the weight, drying, crushing into powder with the particle size of less than 300-500 meshes, and uniformly mixing in a stirring mixer to obtain mixed powder;

2) and adding the mixed powder into an extruder for extrusion granulation, and then carrying out infrared treatment, drying and screening to obtain the soil conditioner.

The soil conditioner comprises short-term strong efficacy components, long-term slow release components and nutrient root system components. The short-term strong efficacy components comprise sulfur powder, the pH of the soil is reduced by adding the sulfur powder, the alkalinity of the soil is further reduced after sulfur bacteria in the soil decompose sulfur and convert the sulfur into sulfuric acid, and the acid is continuously adjusted. The nutrient root system components comprise calcium lignosulphonate, humic acid and fulvic acid, and the calcium lignosulphonate can combine a large amount of humic acid and fulvic acid, so that the growth of the root system can be promoted, and the nutrient absorption efficiency can be improved; meanwhile, calcium lignosulfonate is beneficial to granulation of the fertilizer and promotes disintegration after being dissolved in water. The long-term slow-release components comprise phosphogypsum powder, alkaline soil mainly contains sodium carbonate, sodium bicarbonate and other substances, the substances are alkaline due to hydrolysis, the phosphogypsum powder has certain acidity, and the phosphogypsum powder can be used as a slow-release carrier to gradually reduce the pH value and the alkalization degree of the soil, so that the effect of slowly improving the saline-alkaline property of the soil is achieved.

Further, the growth promoting additive comprises the following components in parts by weight: 10-20 parts of chitosan extracting solution, 10-20 parts of seaweed oligosaccharide extracting solution, 1-3 parts of alanine, 1-3 parts of cysteine, 1-3 parts of histidine, 1-3 parts of lysine, 1-3 parts of glycine, 0.5-5 parts of EDTA chelated iron, 1-5 parts of boric acid, 1-5 parts of manganese sulfate, 1-5 parts of magnesium sulfate and 1-5 parts of zinc sulfate; the chitosan extraction solution contains chitosan with 4-8 polymerization degree more than or equal to 92% and solid content more than or equal to 15%, and the alginate-derived oligosaccharide with 3-6 polymerization degree in the alginate-derived oligosaccharide extraction solution contains alginate-derived oligosaccharide with 3-6 polymerization degree more than or equal to 92% and solid content more than or equal to 15%.

The preparation method of the growth promoting additive comprises the following steps:

1) mixing chitosan extracting solution, brown algae oligosaccharide extracting solution, alanine, cysteine, histidine, lysine, glycine, EDTA chelated iron, boric acid, manganese sulfate, magnesium sulfate and zinc sulfate in proportion, and adjusting the pH value to 3-4 to obtain a mixed solution;

2) and (3) uniformly mixing the mixed solution for 10-30 min by using a dispersing emulsifying machine, and finally preparing the mixed solution into microparticles by using a droplet pressure spray drying tower, wherein the inlet temperature of the drying tower is 145-155 ℃, and the outlet temperature of the drying tower is 70-80 ℃, so that the growth promoting additive is obtained.

The growth promoting additive adopts specific components and adjusts the content of the components, so that the damage to heat sensitive components is effectively avoided, the growth of plant root systems can be promoted by applying the growth promoting additive to plants, the nutrient absorption and transportation efficiency of the root systems is improved, and the yellowing phenomenon caused by the loss of plant silver nutrients is prevented; meanwhile, synthesis of plant chlorophyll can be promoted, photosynthetic efficiency of leaves is improved, and fruit yield is effectively improved.

The alga oligosaccharide is similar to plant hormone, can stimulate the growth of root systems and stems and leaves of crops, and improves the yield of the crops; can induce plants to generate resistance, improve the oxidation resistance of the plants and delay the crop aging. The seaweed oligosaccharide with the polymerization degree of 3-6 has small molecular weight, is not easy to flocculate and precipitate, is easier to be absorbed by plants, and has more remarkable effects of promoting the photosynthesis of the plants and preventing physiological yellowing. The alanine, the cysteine, the histidine, the lysine and the glycine belong to amino acids, and the amino acids are adopted in combination, so that the content of phytoalexin in plants can be improved, and the photosynthesis can be enhanced.

Further, the anti-aging agent comprises the following anti-aging additives in parts by weight: 10-20 parts of brown algae oligosaccharide extracting solution, 61-5 parts of vitamin B and 1-5 parts of anhydrous citric acid; wherein the content of the brown algae oligosaccharide with the degree of polymerization of 4-8 in the brown algae oligosaccharide extracting solution is more than or equal to 92%, and the solid content is more than or equal to 18%.

The preparation method of the anti-aging auxiliary agent comprises the following steps:

1) mixing the brown algae oligosaccharide extracting solution, vitamin B6 and anhydrous citric acid according to a proportion to obtain a mixed solution;

2) and (3) uniformly mixing the mixed solution by using a dispersion emulsifying machine for 10-30 min, and finally preparing the mixed solution into micro-particles by using a spray drying tower, wherein the inlet temperature of the drying tower is 145-155 ℃, and the outlet temperature of the drying tower is 70-80 ℃, so that the anti-aging auxiliary agent is obtained.

The anti-aging auxiliary agent is prepared by adding brown algae oligosaccharide extracting solution, vitamin B6 and anhydrous citric acid, and adjusting the respective contents of the three components to realize the synergistic effect of the three components, so that the obtained biological synergistic auxiliary agent can effectively promote the root development of crops, improve the anti-aging capability of the crops, prolong the growth period of the crops and improve the yield of the crops when being applied to the plants.

Further, the rooting-promoting agent comprises the following components in parts by weight: 10-15 parts of indolebutyric acid, 10-15 parts of potassium indolebutyrate, 1-5 parts of alpha-naphthylacetic acid and 1-5 parts of alpha-sodium naphthylacetate. The root growth promoting assistant can effectively promote the development of crop roots and improve the survival rate of crops by adopting specific components and adjusting the content of the components.

Further, 1-5 parts by weight of a chelate fiber phosphate fertilizer auxiliary agent is also included, and the chelate fiber phosphate fertilizer auxiliary agent comprises monoammonium phosphate and chelate cellulose fibers in a mass ratio of 10-15: 1; the chelate cellulose fiber is obtained by acidifying cellulose fiber with lipoic acid and then carrying out condensation reaction with 2- (diphenylphosphino) ethylamine, wherein the effective phosphorus content of monoammonium phosphate is not less than 60%, and the total nitrogen content is not less than 15%. This application uses monoammonium phosphate and chelate type cellulose base fibre to carry out the chelate fiber phosphorus fertilizer auxiliary agent that the complex was made, and this auxiliary agent not only can reduce soil and to the fixed of phosphorus, has prevented the bad consequence that available state phosphorus turns into invalid state phosphorus in a large number, has improved the utilization of crops to phosphorus, but also has solved the easy defect of caking of monoammonium phosphate itself.

The preparation method of the chelate fiber phosphate fertilizer auxiliary agent comprises the following steps:

1) weighing monoammonium phosphate and chelate cellulose fibers according to the mass ratio of 10-15: 1, and drying for later use;

2) adding the dried monoammonium phosphate and the chelate cellulose fiber into a mixer, and mixing at the speed of 20-100 rpm for 0.2-0.5 h to obtain a mixed product;

3) and adding the mixed product into a granulator, granulating at the temperature of 90-120 ℃, cooling, screening and packaging to obtain the chelate fiber phosphate fertilizer auxiliary agent.

The slow-release charring material is prepared by charring 10-12 parts by weight of fennel, 5-10 parts by weight of pokeberry root, 5-8 parts by weight of golden cypress and 5-10 parts by weight of pinellia ternate at 500-800 ℃ for 2.0-3.0 h. The slow release carbide formed by high temperature carbonization has a microporous structure, and the microporous structure can load other raw materials, so that the prepared biological fertilizer has a slow release effect.

In addition, the invention also provides a preparation method of the compound biofertilizer, which comprises the following steps:

1) weighing the raw materials in parts by weight;

3) adding a formula amount of compound microorganism bacteria and a proper amount of water into a fermentation tank, uniformly stirring, fermenting and decomposing at the fermentation temperature of 20-60 ℃ for 25-35 days, and naturally airing after fermentation is finished to obtain a biological fertilizer mixture;

For further understanding of the present invention, the compound biofertilizer and its preparation method are described in detail with reference to the following examples, but the scope of the present invention is not limited by the following examples.

Example 1

The compound biological fertilizer comprises the following components in parts by weight:

30 parts of organic fermentation product, 60 parts of inorganic fermentation product, 5 parts of compound microorganism bacteria, 20 parts of compound nutrient salt, 10 parts of humic acid, 10 parts of amino acid and 5 parts of amino acid chelating agent;

wherein the organic fermentation product is prepared by mixing chicken manure, cow manure and pig manure according to the mass ratio of 1:1: 1; the inorganic fermentation product is prepared by mixing corn straws, wheat bran, rape stalks and rice husks according to the mass ratio of 1:2:1: 1;

the compound nutrient salt is prepared by mixing monopotassium phosphate, magnesium sulfate, calcium carbonate, magnesium sulfate and zinc sulfate according to the mass ratio of 1:1:1:1: 1;

the amino acid chelating agent is prepared by mixing amino acid chelated iron, amino acid chelated copper, amino acid chelated calcium, amino acid chelated magnesium and amino acid chelated zinc according to the mass ratio of 1:1:1:1: 1;

the composite microbial bacteria are prepared by mixing avermectin streptomycete, rhodopseudomonas palustris, bacillus megaterium, lilium violaceum, pasteurella puncture and bacillus subtilis according to the mass ratio of 1:2:1: 2, and the effective viable bacteria number of the avermectin streptomycete, rhodopseudomonas palustris, bacillus megaterium, lilium violaceum, pasteurella puncture and bacillus subtilis is 1 multiplied by 10 ⁹ cfu/mL。

The preparation method of the compound biological fertilizer comprises the following steps:

1) weighing the raw materials in parts by weight;

3) adding the compound microorganism bacteria and a proper amount of water into a fermentation tank, uniformly stirring, fermenting and decomposing at the fermentation temperature of 40 ℃ for 30 days, and naturally airing after fermentation is finished to obtain a biological fertilizer mixture;

Example 2

Different from the embodiment 1, the compound biological fertilizer comprises the following components in parts by weight:

48 parts of organic fermentation product, 100 parts of inorganic fermentation product, 10 parts of compound microorganism bacterium, 40 parts of compound nutrient salt, 20 parts of humic acid, 20 parts of amino acid and 15 parts of amino acid chelating agent;

wherein the organic fermentation product is prepared by mixing chicken manure, cow manure and pig manure according to the mass ratio of 1:2: 2; the inorganic fermentation product is prepared by mixing corn straws, wheat bran, rape stalks and rice husks according to the mass ratio of 1:3:2: 1;

the compound nutrient salt is prepared by mixing monopotassium phosphate, magnesium sulfate, calcium carbonate, magnesium sulfate and zinc sulfate according to the mass ratio of 2:1:1:1: 1;

the amino acid chelating agent is prepared by mixing amino acid chelated iron, amino acid chelated copper, amino acid chelated calcium, amino acid chelated magnesium and amino acid chelated zinc according to the mass ratio of 2:2:1:1: 1;

the composite microbial bacteria are prepared by mixing avermectin streptomycete, rhodopseudomonas palustris, bacillus megaterium, lilium violaceum, pasteurella puncture and bacillus subtilis according to the mass ratio of 1:2: 3: 1:2: 3, and the effective viable bacteria number of the avermectin streptomycete, rhodopseudomonas palustris, bacillus megaterium, lilium violaceum, pasteurella puncture and bacillus subtilis is 3 multiplied by 10 ⁹ cfu/mL。

The rest is the same as embodiment 1, and the description is omitted here.

Example 3

38 parts of organic fermentation product, 80 parts of inorganic fermentation product, 8 parts of compound microorganism bacteria, 30 parts of compound nutrient salt, 15 parts of humic acid, 15 parts of amino acid and 10 parts of amino acid chelating agent;

wherein the organic fermentation product is prepared by mixing chicken manure, cow manure and pig manure according to the mass ratio of 1:1: 2; the inorganic fermentation product is prepared by mixing corn straws, wheat bran, rape stalks and rice husks according to the mass ratio of 1:2:1: 1;

the composite microbial bacteria are prepared by mixing streptomyces avermitilis, rhodopseudomonas palustris, bacillus megaterium, rhodosporidium lilacinum, pasteurella puncture and bacillus subtilis according to the mass ratio of 1:3: 1:3, and the effective viable bacteria number of the streptomyces avermitilis, the rhodopseudomonas palustris, the bacillus megaterium, the rhodosporidium lilacinum, the pasteurella puncture and the bacillus subtilis is 2 multiplied by 10 ⁹ cfu/mL。

The rest is the same as embodiment 1, and the description is omitted here.

The biofertilizers prepared in the embodiments 1-3 and a commercially available common organic fertilizer are respectively adopted to irrigate corns for effect tests, and the test results are as follows:

numbering	Fertilizing amount kg/mu	Corn yield kg/mu	Increased yield%
				Example 1	25	920	25.2
Example 2	25	932	26.8
				Example 3	25	968	31.7
Common organic fertilizer	25	735	8.5

From the above test results, it was found that the yield of corn could be increased by irrigating corn with the bio-fertilizer of the present invention, and the yield of the bio-fertilizer obtained in example 3 was the highest.

Example 4

The difference from example 1 is:

the compound biofertilizer also comprises a color conversion promoting additive which is composed of the following components in parts by weight: 15 parts of brown algae oligosaccharide extracting solution, 1 part of phenylalanine, 1 part of methionine, 1 part of arginine, 1 part of leucine, 0.5 part of astacin, 2 parts of vitamin C, 3 parts of manganese sulfate and 3 parts of magnesium sulfate; wherein the content of the brown algae oligosaccharide with the degree of polymerization of 4-8 in the brown algae oligosaccharide extracting solution is 95%, and the solid content is 18%.

1) mixing the brown algae oligosaccharide extracting solution, phenylalanine, methionine, arginine, leucine, astaxanthin, vitamin C, manganese sulfate and magnesium sulfate in proportion, and adjusting the pH value to 3 to obtain a mixed solution;

2) and stirring the mixed solution for 20min by using a dispersing emulsifying machine, uniformly mixing, and finally preparing into micro-particles by using a droplet pressure spray drying tower, wherein the inlet temperature of the drying tower is 150 ℃, and the outlet temperature of the drying tower is 75 ℃, so that the color conversion promoting additive is obtained.

The preparation method of the compound biofertilizer comprises the following steps:

1) weighing the raw materials in parts by weight;

3) adding the compound microorganism bacteria and a proper amount of water into a fermentation tank, uniformly stirring, fermenting and decomposing, wherein the fermentation temperature is 40 ℃, fermenting for 30 days, and naturally airing after fermentation is finished to obtain a biological fertilizer mixture;

4) adding the biological fertilizer mixture, the amino acid chelating agent and the color conversion promoting additive into a stirrer, uniformly mixing, crushing and screening into powder to obtain the compound biological fertilizer.

The rest is the same as embodiment 1, and the description is omitted here.

The effect tests were carried out on the complex biofertilizers obtained in example 1 and example 4, respectively.

The test method comprises the following steps: the compound biofertilizers prepared in example 1 and example 4 were respectively subjected to a fertilizer effect test on greenhouse cherry tomatoes (Jingdan No. 1), and the application method is as follows: spraying 3 times (1200 times) of the cherry tomato fruits from the color-changing period to the mature period at an interval of 7 days.

The test result shows that compared with the example 1, the biological fertilizer is added with the color transformation promoting additive for processing, so that the cherry and tomato fruits are more uniformly colored, and the first spike fruit coloring period is advanced by 3 days; lycopene in the fruits is remarkably improved to 58.2mg/kg, which is improved by more than 15% compared with that of the fruit in example 1; the content of soluble sugar in the fruit reaches 9 percent, and is increased by more than 10 percent compared with the example 1, and the quality of the fruit is obviously improved.

Example 5

The difference from example 1 is:

the compound biofertilizer also comprises a sweetening aid which is composed of the following components in parts by weight: 15 parts of seaweed oligosaccharide extracting solution, 3 parts of cysteine, 3 parts of proline and 3 parts of monopotassium phosphate; wherein the content of the algal oligosaccharide with the degree of polymerization of 3-6 in the algal oligosaccharide extracting solution is 95%, and the solid content is 15%.

The preparation method of the sweetening aid comprises the following steps:

1) mixing the seaweed oligosaccharide extracting solution, cysteine, proline and monopotassium phosphate in proportion, and then adjusting the pH value to 3 to obtain a mixed solution;

2) and stirring the mixed solution for 20min by using a dispersing emulsifying machine, uniformly mixing, and finally preparing into micro-particles by using a micro-droplet pressure spray drying tower, wherein the inlet temperature of the drying tower is 150 ℃, and the outlet temperature of the drying tower is 85 ℃, so that the sweetening aid is obtained.

1) weighing the raw materials in parts by weight;

4) adding the biological fertilizer mixture, the amino acid chelating agent and the sweetening aid into a stirrer, uniformly mixing, crushing and screening into powder to obtain the compound biological fertilizer.

The rest is the same as embodiment 1, and the description is omitted here.

The effect tests were carried out on the complex biofertilizers obtained in example 1 and example 5, respectively.

The test method comprises the following steps: the compound biological fertilizers prepared in example 1 and example 5 were respectively subjected to an effect test on the netted melon in the following way: spraying 1000 times of the fertilizer 2 times before the mature period of the fruits at an interval of 10 days.

The test result shows that: compared with the example 1, the melon fruit quality is obviously improved: the soluble solid content of the fruit is 12.5 percent, which is increased by 8.5 percent compared with that of the fruit in example 1; the content of soluble sugar reaches 12.5 percent, which is increased by 16.2 percent compared with the example 1; the titratable acid content is 0.15 percent, which is reduced by 15.0 percent compared with that of the embodiment 1; the content of vitamin C is 22.5mg/kg, which is increased by 20.6 percent compared with the content of the vitamin C in the example 1.

Example 6

The difference from example 1 is:

the compound biological fertilizer also comprises a soil conditioner which is composed of the following components in parts by weight: 15 parts of phosphogypsum powder, 5 parts of sulfur powder, 3 parts of soil humic acid, 3 parts of fulvic acid and 5 parts of calcium lignosulphonate; wherein the particle size of the phosphogypsum powder is 160-180 mu m.

The preparation method of the soil conditioner comprises the following steps:

1) weighing the raw materials in parts by weight;

2) mixing organic fermentation products, inorganic fermentation products, humic acid, compound nutrient salt and a soil conditioner, then feeding the mixture into a crusher for crushing, and feeding the crushed material into a fermentation tank;

The rest is the same as embodiment 1, and the description is omitted here.

The effect tests were carried out on the complex biofertilizers obtained in example 1 and example 6, respectively.

The test method comprises the following steps: the compound biofertilizers prepared in example 1 and example 6 were respectively tested on corn in the following manner: after planting the corn, the corn is sprayed for 2 times in 1000 times with an interval of 10 days.

The test results are as follows:

numbering	Example 1	Example 6
			Detection of pH before corn planting	7.58	7.55
Detection of pH after corn planting	7.24	6.62
			Corn yield (kg/mu)	935	975

The test results show that the pH value of the saline-alkali soil is reduced after the biological fertilizer is added with the soil conditioner, and the biological fertilizer prepared in the embodiment 6 of the invention has a good repairing effect on medium and light saline-alkali soil, and is more suitable for the growth of crops after being repaired.

Example 7

The difference from example 1 is:

the compound biofertilizer also comprises a growth promoting additive which is composed of the following components in parts by weight: 15 parts of chitosan extracting solution, 15 parts of seaweed oligosaccharide extracting solution, 2 parts of alanine, 2 parts of cysteine, 2 parts of histidine, 2 parts of lysine, 2 parts of glycine, 3 parts of EDTA chelated iron, 3 parts of boric acid, 3 parts of manganese sulfate, 3 parts of magnesium sulfate and 3 parts of zinc sulfate; the chitosan extraction solution contains 95% of chitosan with a degree of polymerization of 4-8 and 15% of solid content, and the alginate oligosaccharide extraction solution contains 95% of alginate oligosaccharide with a degree of polymerization of 3-6 and 15% of solid content.

1) mixing chitosan extract, alginate oligosaccharide extract, alanine, cysteine, histidine, lysine, glycine, EDTA chelated iron, boric acid, manganese sulfate, magnesium sulfate and zinc sulfate in proportion, and adjusting the pH value to 3 to obtain a mixed solution;

2) and stirring the mixed solution for 20min by using a dispersing emulsifying machine, uniformly mixing, and finally preparing into microparticles by using a droplet pressure spray drying tower, wherein the inlet temperature of the drying tower is 150 ℃, and the outlet temperature of the drying tower is 75 ℃, so that the growth promoting additive is obtained.

1) weighing the raw materials in parts by weight;

4) adding the biological fertilizer mixture, the amino acid chelating agent and the growth promoting additive into a stirrer, uniformly mixing, crushing and screening into powder to obtain the compound biological fertilizer.

The rest is the same as embodiment 1, and the description is omitted here.

The effect tests were carried out on the complex biofertilizers obtained in example 1 and example 7, respectively.

The test method comprises the following steps: the compound biofertilizers prepared in example 1 and example 7 were respectively subjected to fertilizer efficiency tests on greenhouse cucumbers in the following manner: spraying 2 times at the fruit bearing period of 1000 times, and 7 days at intervals.

The test result shows that compared with the example 1, the growth promotion additive is added into the biological fertilizer for application, so that the growth vigor of cucumber plants and the growth vigor of root systems are both obviously improved. The growth indexes of the cucumber plant height, the stem thickness, the single plant yield and the like are respectively improved by 6.5 percent, 8.8 percent and 10.5 percent compared with the growth indexes of the cucumber plant 1; the total root length, total surface area and root volume of cucumber are increased by 18.2%, 18.8% and 19.5% compared with example 1.

Example 8

The difference from example 1 is:

the compound biofertilizer also comprises an anti-aging additive which is composed of the following components in parts by weight: 15 parts of brown algae oligosaccharide extracting solution, 63 parts of vitamin B and 3 parts of anhydrous citric acid; wherein the content of the brown algae oligosaccharide with the degree of polymerization of 4-8 in the brown algae oligosaccharide extracting solution is 95%, and the solid content is 18%.

2) and stirring the mixed solution for 20min by using a dispersion emulsifying machine, uniformly mixing, and finally preparing into micro-particles by using a spray drying tower, wherein the inlet temperature of the drying tower is 150 ℃, and the outlet temperature of the drying tower is 75 ℃ to obtain the anti-aging auxiliary agent.

1) weighing the raw materials in parts by weight;

4) adding the biological fertilizer mixture, the amino acid chelating agent and the anti-aging auxiliary agent into a stirrer, uniformly mixing, crushing and screening into powder to obtain the compound biological fertilizer.

The rest is the same as embodiment 1, and the description is omitted here.

Example 9

The difference from example 1 is:

the compound biofertilizer also comprises a root growth promoting auxiliary agent which is composed of the following components in parts by weight: 12 parts of indolebutyric acid, 12 parts of potassium indolebutyrate, 3 parts of alpha-naphthylacetic acid and 3 parts of alpha-sodium naphthylacetate.

1) weighing the raw materials in parts by weight;

4) adding the biological fertilizer mixture, the amino acid chelating agent and the root growth promoting auxiliary agent into a stirrer, uniformly mixing, crushing and screening into powder to obtain the compound biological fertilizer.

The rest is the same as embodiment 1, and the description is omitted here.

Example 10

The difference from example 1 is:

the compound biofertilizer also comprises an anti-aging auxiliary agent and a root growth promoting auxiliary agent, wherein the anti-aging auxiliary agent comprises the following components in parts by weight: 15 parts of brown algae oligosaccharide extracting solution, 63 parts of vitamin B and 3 parts of anhydrous citric acid; wherein the content of the brown algae oligosaccharide with the degree of polymerization of 4-8 in the brown algae oligosaccharide extracting solution is 95%, and the solid content is 18%.

Wherein the rooting-promoting additive consists of the following components in parts by weight: 12 parts of indolebutyric acid, 12 parts of potassium indolebutyrate, 3 parts of alpha-naphthylacetic acid and 3 parts of alpha-sodium naphthylacetate.

1) weighing the raw materials in parts by weight;

4) adding the biological fertilizer mixture, amino acid chelating agent, anti-aging auxiliary agent and root growth promoting auxiliary agent into a stirrer, uniformly mixing, crushing and screening into powder to obtain the compound biological fertilizer.

The rest is the same as embodiment 1, and the description is omitted here.

The effect tests were carried out on the composite biofertilizers obtained in examples 1 and 8 to 10, respectively.

The test method comprises the following steps: the compound biofertilizers prepared in the embodiment 1 and the embodiments 8-10 are applied to the small rape in a drop irrigation mode in the 3-leaf stage, the dosage per mu is 25g, the dilution multiple is 2000 times, and after the test harvest, 20 small rape are selected repeatedly every time to measure indexes such as plant height, root length, root activity, biomass and the like. The results of the test measurements are shown in the following table:

according to test results, after the anti-aging auxiliary and/or the root growth promoting auxiliary are added to the biological fertilizer, the crops have good root promoting, anti-aging and yield increasing effects, wherein when the anti-aging auxiliary and the root growth promoting auxiliary are added to the biological fertilizer at the same time, the root growth promoting, anti-aging and yield increasing effects are optimal.

Example 11

The difference from example 1 is:

the compound biofertilizer also comprises 3 parts by weight of chelate fiber phosphate fertilizer auxiliary agent, wherein the chelate fiber phosphate fertilizer auxiliary agent comprises monoammonium phosphate and chelate cellulose fiber in a mass ratio of 12: 1; the chelate cellulose fiber is obtained by acidifying cellulose fiber with lipoic acid and then carrying out condensation reaction with 2- (diphenylphosphino) ethylamine, wherein the effective phosphorus content of monoammonium phosphate is 60% and the total nitrogen content is 15%.

1) weighing monoammonium phosphate and chelating cellulose fiber according to the mass ratio of 12:1, and drying for later use;

2) adding the dried monoammonium phosphate and the chelate cellulose fiber into a mixer, and mixing at the speed of 80rpm for 0.4h to obtain a mixed product;

3) and adding the mixed product into a granulator, granulating at the temperature of 100 ℃, cooling, screening and packaging to obtain the chelate fiber phosphate fertilizer auxiliary agent.

1) weighing the raw materials in parts by weight;

4) adding the biological fertilizer mixture, amino acid chelating agent and chelate fiber phosphate fertilizer auxiliary agent into a stirrer, uniformly mixing, crushing and screening into powder to obtain the compound biological fertilizer.

The rest is the same as embodiment 1, and the description is omitted here.

The effect tests were carried out on the complex biofertilizers obtained in example 1 and example 11, respectively.

The test method comprises the following steps: the compound biofertilizers prepared in example 1 and example 11 were respectively subjected to an effect test on wheat in the following manner: 50 kg/mu of fertilizer is applied at one time during the period of three leaves and one heart.

The utilization rate (%) of plant phosphorus is calculated by detecting the phosphorus content in the fertilizer, the phosphorus content in soil before planting and the phosphorus content in soil at harvest, namely the utilization rate (%) of plant phosphorus (phosphorus content in soil + phosphorus content in fertilizer-phosphorus content in soil at harvest)/(phosphorus content in soil + phosphorus content in fertilizer) x 100%, and the related results are shown in the following table:

number of	Plant phosphorus utilization (%)	Yield (kg/mu)
			Example 1	25.4	574.2
Example 11	32.8	582.6

From the test results, it can be seen that the plant phosphorus utilization rate of the biofertilizer of example 11 of the present invention was increased by about 30% compared to example 1, and the yield was higher than that of example 1, indicating that the reason for the low yield is probably due to the low phosphorus utilization rate, and reflecting that the biofertilizer used in example 11 has better applicability and can provide sufficient phosphorus to the plant even if no additional phosphate fertilizer is added.

Example 12

The difference from example 1 is:

the compound biofertilizer further comprises a slow release carbide, wherein the slow release carbide is prepared by carbonizing 10-12 parts by weight of fennel, 5-10 parts by weight of pokeberry root, 5-8 parts by weight of golden cypress and 5-10 parts by weight of pinellia ternate at 500-800 ℃ for 2.0-3.0 h.

1) weighing the raw materials in parts by weight;

4) adding the biological fertilizer mixture, the amino acid chelating agent and the slow-release carbide into a stirrer, uniformly mixing, crushing and screening into powder to obtain the compound biological fertilizer.

The rest is the same as embodiment 1, and the description is omitted here.

Example 13

The difference from example 1 is:

the compound biofertilizer also comprises a growth promoting additive, an anti-aging additive, a root promoting additive, a chelate fiber phosphate fertilizer additive and a slow-release carbide.

The content and preparation of the growth-promoting assistant are the same as those of example 7, the content and preparation of the anti-aging assistant are the same as those of example 8, the content and preparation of the root-promoting assistant are the same as those of example 9, the content and preparation of the chelate fiber phosphate fertilizer assistant are the same as those of example 11, and the content and preparation of the slow-release carbide are the same as those of example 12.

The rest is the same as embodiment 1, and the description is omitted here.

Example 14

The difference from example 1 is:

the compound biofertilizer also comprises a color-conversion-promoting additive, a sweetening additive, a soil conditioner, a growth-promoting additive, an anti-aging additive, a root-growth-promoting additive, a chelate fiber phosphate fertilizer additive and a slow-release carbide.

Wherein, the content and the preparation of the color transfer promoter are the same as those of the example 4, the content and the preparation of the sweetening promoter are the same as those of the example 5, the content and the preparation of the soil conditioner are the same as those of the example 6, the content and the preparation of the growth promoting promoter are the same as those of the example 7, the content and the preparation of the anti-aging promoter are the same as those of the example 8, the content and the preparation of the root growth promoting promoter are the same as those of the example 9, the content and the preparation of the chelate fiber phosphate fertilizer promoter are the same as those of the example 11, and the content and the preparation of the slow-release carbide are the same as those of the example 12.

The rest is the same as embodiment 1, and the description is omitted here.

The effect tests were carried out on the composite biofertilizers obtained in example 1 and examples 12 to 14, respectively.

The test method comprises the following steps: the compound biofertilizers prepared in the embodiment 1 and the embodiments 12-14 are applied to the small rape in a drop irrigation mode in the 3-leaf stage, the dosage per mu is 25g, the dilution multiple is 2000 times, and after the test harvest, 20 small rape are selected repeatedly each time to measure indexes such as plant height, root length, root activity, biomass and the like. The results of the test measurements are shown in the following table:

according to the test results, the biofertilizers of the embodiments 12 to 14 of the invention enable crops to have good effects of promoting root system development, resisting aging and increasing yield, wherein the biofertilizers of the embodiments 13 to 14 have better effects of promoting root growth, resisting aging and increasing yield when applied, and particularly the biofertilizers of the embodiment 14 have the best effects of promoting root growth, resisting aging and increasing yield when applied.

Variations and modifications to the above-described embodiments may also occur to those skilled in the art, which fall within the scope of the invention as disclosed and taught herein. Therefore, the present invention is not limited to the above-mentioned embodiments, and any obvious improvement, replacement or modification made by those skilled in the art based on the present invention is within the protection scope of the present invention. Furthermore, although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.

Claims

1. The compound biofertilizer is characterized by comprising the following components in parts by weight:

30-48 parts of organic fermentation product, 60-100 parts of inorganic fermentation product, 5-10 parts of compound microorganism bacteria, 20-40 parts of compound nutrient salt, 10-20 parts of humic acid, 10-20 parts of amino acid and 5-15 parts of amino acid chelating agent;

the organic fermentation product is prepared by mixing chicken manure, cow manure and pig manure according to the mass ratio of 1: 1-2; the inorganic fermentation product is prepared by mixing corn stalks, wheat bran, rape stalks and rice husks according to a mass ratio of 1: 2-3: 1-2: 1;

the amino acid chelating agent is prepared by mixing amino acid chelated iron, amino acid chelated copper, amino acid chelated calcium, amino acid chelated magnesium and amino acid chelated zinc according to the mass ratio of 1-2: 1:1: 1;

2. The compound biofertilizer of claim 1, characterized in that: the color transfer promoter also comprises the following color transfer promoter in parts by weight: 10-20 parts of brown algae oligosaccharide extracting solution, 0.5-1.5 parts of phenylalanine, 0.5-1.5 parts of methionine, 0.5-1.5 parts of arginine, 0.5-1.5 parts of leucine, 0.5-1 part of astacin, 1-3 parts of vitamin C, 1-5 parts of manganese sulfate and 1-5 parts of magnesium sulfate; wherein the content of the brown algae oligosaccharide with the degree of polymerization of 4-8 in the brown algae oligosaccharide extracting solution is more than or equal to 92%, and the solid content is more than or equal to 18%.

3. The compound biofertilizer of claim 1, characterized in that: the sweet-enhancing agent also comprises the following sweet-enhancing auxiliary agents in parts by weight: 10-20 parts of seaweed oligosaccharide extracting solution, 1-5 parts of cysteine, 1-5 parts of proline and 1-5 parts of monopotassium phosphate; wherein the content of the algal oligosaccharide with the degree of polymerization of 3-6 in the algal oligosaccharide extracting solution is more than or equal to 92%, and the solid content is more than or equal to 15%.

4. The compound biofertilizer of claim 1, characterized in that: the soil conditioner also comprises a soil conditioner consisting of the following components in parts by weight: 10-20 parts of phosphogypsum powder, 1-10 parts of sulfur powder, 1-5 parts of soil humic acid, 1-5 parts of fulvic acid and 1-10 parts of calcium lignosulfonate; wherein the particle size of the phosphogypsum powder is 160-180 mu m.

5. The compound biofertilizer of claim 1, characterized in that: the growth promoting agent also comprises the following growth promoting additives in parts by weight: 10-20 parts of chitosan extracting solution, 10-20 parts of seaweed oligosaccharide extracting solution, 1-3 parts of alanine, 1-3 parts of cysteine, 1-3 parts of histidine, 1-3 parts of lysine, 1-3 parts of glycine, 0.5-5 parts of EDTA (ethylene diamine tetraacetic acid) chelated iron, 1-5 parts of boric acid, 1-5 parts of manganese sulfate, 1-5 parts of magnesium sulfate and 1-5 parts of zinc sulfate; the chitosan extraction solution contains chitosan with 4-8 polymerization degree more than or equal to 92% and solid content more than or equal to 15%, and the alginate-derived oligosaccharide with 3-6 polymerization degree in the alginate-derived oligosaccharide extraction solution contains alginate-derived oligosaccharide with 3-6 polymerization degree more than or equal to 92% and solid content more than or equal to 15%.

6. The compound biofertilizer of claim 1, characterized in that: the anti-aging coating also comprises an anti-aging auxiliary agent which is composed of the following components in parts by weight: 10-20 parts of brown algae oligosaccharide extracting solution, 61-5 parts of vitamin B and 1-5 parts of anhydrous citric acid; wherein the content of the brown algae oligosaccharide with the degree of polymerization of 4-8 in the brown algae oligosaccharide extracting solution is more than or equal to 92%, and the solid content is more than or equal to 18%.

7. The compound biofertilizer of claim 1, characterized in that: the rooting agent also comprises the following rooting-promoting additives in parts by weight: 10-15 parts of indolebutyric acid, 10-15 parts of indolebutyric acid potassium, 1-5 parts of alpha-naphthylacetic acid and 1-5 parts of alpha-sodium naphthylacetate.

8. The compound biofertilizer of claim 1, characterized in that: the fiber chelating phosphate fertilizer additive comprises 1-5 parts by weight of chelating fiber phosphate fertilizer additive, wherein the chelating fiber phosphate fertilizer additive comprises monoammonium phosphate and chelating cellulose fiber in a mass ratio of 10-15: 1; the chelate cellulose fiber is obtained by acidifying cellulose fiber with lipoic acid and then carrying out condensation reaction with 2- (diphenylphosphino) ethylamine, wherein the effective phosphorus content of monoammonium phosphate is not less than 60%, and the total nitrogen content is not less than 15%.

9. The compound biofertilizer of claim 1, characterized in that: the slow-release charring material is prepared by charring 10-12 parts by weight of fennel, 5-10 parts by weight of pokeberry root, 5-8 parts by weight of golden cypress and 5-10 parts by weight of pinellia ternate at 500-800 ℃ for 2.0-3.0 h.

10. A method for preparing a compound biofertilizer according to any one of claims 1 to 9, characterized by comprising the steps of:

1) weighing the raw materials in parts by weight;