CN114869230B - In-vivo optical imaging device suitable for animal intestinal tissue - Google Patents
In-vivo optical imaging device suitable for animal intestinal tissue Download PDFInfo
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- CN114869230B CN114869230B CN202210431983.9A CN202210431983A CN114869230B CN 114869230 B CN114869230 B CN 114869230B CN 202210431983 A CN202210431983 A CN 202210431983A CN 114869230 B CN114869230 B CN 114869230B
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 208000003098 Ganglion Cysts Diseases 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
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Abstract
The invention discloses an in-vivo optical imaging device suitable for animal intestinal tissues, which comprises an abdominal window ring and a clamping groove, wherein the clamping groove comprises a U-shaped groove body and a fixing pile which are integrally formed, the fixing pile extends from the bottom surface of the U-shaped groove body to the direction of an opening at the top, and two side walls of the U-shaped groove body are respectively provided with a clamping piece; the abdomen window ring includes annular window body and from annular window body inside wall different degree of depth positions to annular ring center first annular bulge and second annular bulge that extend, has the clearance between two archs in order to form the assembly groove, and the protruding inboard breach that sets up of second annular bulge has the symmetry, breach and card looks adaptation so that the card of draw-in groove inlay in the assembly inslot through the breach, and annular window body outside wall is formed with the seam groove of inwards sunkening. The fixed piles of the clamping grooves are used for hooking the target objects, and then the clamping pieces on the clamping grooves are embedded in the assembly grooves through the gaps, so that the situation that the original research target is lost due to intestinal peristalsis is avoided, and the fixed-point long-time imaging research on the target intestinal section is facilitated.
Description
Technical Field
The invention belongs to the technical field of medical instruments and appliances, and particularly relates to an in-vivo optical imaging device suitable for animal intestinal tissues.
Background
The intestinal tract is an important component of the digestive tract. In humans, the gut contains a total of about 5 hundred million neurons of about 20 different functional classes. The intestine is also known as the second brain due to its size, functional complexity and some structural similarity. Meanwhile, the intestinal tract is the largest immune organ in the human body, and about 70% of immune cells exist in the intestinal tract of the human body. Therefore, the optical imaging research on the experimental animal at the body level plays an important role in researching the roles of the intestinal tract in the physiological activities such as digestion, immunity and the like, and has important significance on the intestinal diseases and related diseases caused by the intestinal dysfunction.
At present, the intestinal structure function is studied at the body level by utilizing an optical imaging technology, mainly by means of an abdominal window, and the window is sutured on the abdomen of an experimental animal through an operation, wherein the mechanical structure has good biocompatibility (mainly of titanium alloy, resin and other materials). The abdominal window isolates the external environment and provides an imaging observation window, so that the experimental animals can survive for a plurality of weeks, and a powerful tool is provided for the in-vivo optical imaging research of intestinal tracts. However, this device does not allow long-term optical imaging studies of the fixed location of intestinal tissue, subject to intestinal peristalsis.
Disclosure of Invention
In view of the above-mentioned drawbacks or improvements of the prior art, the present invention provides an in-vivo optical imaging device suitable for animal intestinal tissue, which aims to effectively fix target intestinal tissue and realize long-time optical imaging study of target intestinal segments.
To achieve the above object, according to one aspect of the present invention, there is provided an in-vivo optical imaging device suitable for animal intestinal tissue, comprising a window ring and a clamping groove for cooperation, wherein:
the clamping groove comprises an integrally formed U-shaped groove body and fixing piles, wherein the U-shaped groove body is formed by surrounding a bottom surface and two opposite side walls, the fixing piles extend from the bottom surface of the U-shaped groove body to the direction of an opening at the top, and the two side walls of the U-shaped groove body are respectively provided with a clamping piece which extends along the direction deviating from the U-shaped groove body;
The abdomen window ring includes annular window form and extends the first annular bulge and the second annular bulge that form from the different degree of depth positions of annular window inner side wall to the ring center, first annular bulge with the second annular bulge is followed the direction of the front directional back of annular window form sets up side by side and has the clearance in order to form the assembly groove, first annular bulge is apart from the positive degree of depth of annular window form is greater than 0, the protruding inboard breach that sets up of second annular, the breach with card looks adaptation is so that the card of draw-in groove passes through the protruding breach of second annular is inlayed in the assembly groove, the outer lateral wall of annular window is formed with the seam groove of inwards sunkening.
In one embodiment, the outer edge of the second annular projection and the outer edge of the first annular projection are axially aligned along the annular window, and an annular window inner diameter between the first annular projection and the second annular projection is greater than an annular window inner diameter between the first annular projection and the annular window front face.
In one embodiment, the fixing pile is located at the middle position of the bottom surface of the U-shaped groove body, and the width of the fixing pile is smaller than that of the bottom surface of the U-shaped groove body.
In one embodiment, the edge of the card is arcuate and the edge of the notch is arcuate to fit the card.
In one embodiment, the abdominal window ring further comprises symmetrically disposed fixed plugs extending upwardly from the annular window body, each fixed plug extending a height above the front face of the annular window body.
In one embodiment, the orthographic projections of the symmetrically disposed securing pegs and notches are all located on a straight line of the same diameter of the annular window.
In one embodiment, the slide glass comprises a first annular protrusion, a second annular protrusion, a clamping spring, a slide glass body and a slide glass cover, wherein the first annular protrusion is provided with a first end and a second end, the outer side edge of the clamping spring is provided with an arc shape, the angle of the arc shape is larger than 180 degrees, and the clamping spring is matched with the inner side of the annular window body and fixes the slide glass between the clamping spring and the first annular protrusion through friction force with the inner side wall of the annular window body.
In one embodiment, the width of the first end and the second end of the clamp spring is larger than the width of the middle circular arc-shaped connecting portion, and each end is provided with a clamping through hole.
In one embodiment, the fixing plate is matched with the abdominal window ring, the fixing plate comprises a perspective through hole and a positioning hole matched with the fixing plug, the fixing plug is used for being inserted into the positioning hole to be fixed with the fixing plate, and after the fixing plug is used for being inserted into the positioning hole, the abdominal window ring is aligned with the perspective through hole and an image inside the abdominal window ring is observed through the perspective through hole.
In one embodiment, the curved position of the clamping groove is in a smooth arc shape.
In general, the above technical solutions conceived by the present invention, compared with the prior art, enable the following beneficial effects to be obtained:
Through design abdomen window ring and with the supporting draw-in groove that uses of abdomen window ring, wherein, be close to positive first annular bulge on the abdomen window ring and be used for supporting the slide, the assembly groove that first annular bulge and second annular bulge enclose is used for the equipment draw-in groove. The draw-in groove design is the U type and has the spud pile, and the side projection of U type cell body and spud pile is "mountain" font, and two lateral walls of U type cell body are formed with first card and second card. After the abdominal window ring is sutured with the study object through the suture groove, the clamping groove is stretched into the interior through the abdominal window ring window by using tweezers, the fixing piles of the clamping groove are hooked on the target object (intestinal tract), then the card on the clamping groove is stretched into the assembly groove through the notch of the second annular bulge and rotated for a certain angle, the card is embedded in the assembly groove, the target object is fixed in the clamping groove, the original study target is prevented from being lost due to intestinal peristalsis, and the fixed-point long-time imaging study on the target intestinal segment is facilitated.
Drawings
Fig. 1 is a schematic structural diagram of a card slot according to an embodiment, wherein fig. 1 a is a top view of the card slot, fig. 1B is a side view of the card slot, fig. 1C is a top view of the card slot, and fig. 1D is a three-dimensional perspective view of the card slot;
Fig. 2 is a schematic structural view of an abdominal window ring according to an embodiment, wherein fig. 2a is a top view of the abdominal window ring, fig. 2B is a top view of a clamping groove, fig. 2C is a side view of the clamping groove, and fig. 2D and fig. 2E are three-dimensional perspective views of the clamping groove at different angles, respectively;
FIG. 3 is a perspective view of an assembled structure of a clamping groove and a belly ring according to an embodiment;
Fig. 4 is a schematic diagram of an assembled structure of a clamping groove and an abdominal window ring according to an embodiment, wherein fig. 4a is a top view of the assembled structure, fig. 4B is a side view of the assembled structure, and fig. 4C is a top view of the assembled structure;
FIG. 5 is a schematic diagram of an assembled structure of a clamping groove and a ventral window ring for a subject according to an embodiment;
FIG. 6 is a schematic structural view of a fixing plate according to an embodiment;
FIG. 7 is a schematic illustration of a fixation plate secured to a three-dimensional translation stage according to an embodiment;
FIG. 8 is a diagram of the intercolonic mydriasis architecture obtained by optical imaging, according to one embodiment.
The same reference numbers are used throughout the drawings to reference like elements or structures, wherein:
100. a clamping groove; 110. fixing piles; 120. a card; 130. the side wall of the U-shaped groove;
200. An abdominal window ring; 210. an annular window; 220. a first annular projection; 230. a second annular projection; 231. a notch; 240. a suture groove; 250. fixing the plug;
300. a slide;
400. clamping springs;
500. A fixing plate; 510. perspective through holes; 520. positioning holes; 530. fixing the screw holes;
600. A bread board;
700. A three-dimensional translation stage.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention. In addition, the technical features of the embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
The in-vivo optical imaging device suitable for animal intestinal tissues comprises an abdominal window ring and a clamping groove which are matched for use.
Fig. 1 is a schematic structural diagram of a card slot 100 according to an embodiment, wherein fig. 1a is a top view of the card slot, fig. 1B is a side view of the card slot, fig. 1C is a top view of the card slot, and fig. 1D is a three-dimensional perspective view of the card slot. Specifically, the clamping groove 100 comprises an integrally formed U-shaped groove body and a fixing pile 110, the U-shaped groove body is formed by surrounding a bottom surface and two opposite U-shaped groove side walls 130, the fixing pile 110 extends from the bottom surface of the U-shaped groove body to the direction of the top opening, and the side projections of the U-shaped groove body and the fixing pile are in a shape of a Chinese character 'shan'. Two U-shaped groove side walls 130 of the U-shaped groove body are respectively provided with a card 120 which extends along the direction deviating from the U-shaped groove body, wherein each side wall is provided with a card.
Fig. 2 is a schematic structural diagram of an abdominal ring 200 according to an embodiment, wherein fig. 2 a is a top view of the abdominal ring, fig. 2B is a top view of the clamping groove, fig. 2C is a side view of the clamping groove, and fig. 2D and fig. 2E are three-dimensional perspective views of the clamping groove at different angles. Specifically, the abdominal window ring 200 includes an integrally formed annular window 210, a first annular protrusion 220 and a second annular protrusion 230, where the first annular protrusion 220 and the second annular protrusion 230 are located at different depth positions on the inner side wall of the annular window 210 and protrude from the inner side wall of the annular window 210. Defining one surface of the annular window 210 as a front surface, the surface opposite to the front surface is a back surface, and the first annular protrusion 220 and the second annular protrusion 230 are arranged side by side with a gap from the front surface to the back surface, and the gap forms an assembly groove. In one embodiment, the second annular protrusion 230 has a larger annular width than the first annular protrusion 220 and the same inner diameter, but the second annular protrusion 230 has an outer diameter larger than the outer diameter of the first annular protrusion 220, i.e., the inner diameter of the inner sidewall of the annular window 210 varies in size, the inner diameter of the inner sidewall between the first annular protrusion 220 and the back surface of the window is relatively large, and the inner diameter of the inner sidewall between the first annular protrusion 220 and the front surface of the window is relatively large, thereby increasing the depth of the fitting groove. And the depth of the first annular protrusion 220 from the front surface of the annular window is greater than 0, namely, a first-stage step is formed and is used for supporting a slide, so that the slide can be conveniently replaced and taken down to adapt to various imaging modes, such as confocal endoscope imaging and the like. The inner side of the second annular protrusion 230 is further provided with symmetrically arranged notches 231, for example, two notches are provided in fig. 2, and the two notches are respectively adapted to the card 120 on the card slot 100, so that the card 120 of the card slot 100 is embedded in the assembly slot through the notches 231 of the second annular protrusion 230, and the assembly of the card slot 100 and the abdominal window ring 200 is realized. At the same time, the outer side wall of the annular window 210 is also formed with an inwardly concave suture groove 240 for suturing with the subject.
Fig. 3 is a perspective view of an assembly structure of the clamping groove and the abdominal window ring, fig. 4 is a top view of the assembly structure, fig. 4 is a side view of the assembly structure, and fig. 4 is a top view of the assembly structure. The abdominal window ring 200 may be sewn with the subject through a sewing groove, the clamping groove 100 is extended into the interior through the abdominal window ring window, the fixing pile of the clamping groove 100 is hooked on the target (intestinal tract), and then the card on the clamping groove is extended into the assembly groove through the notch of the second annular protrusion and rotated for a certain angle, so that the card is embedded in the assembly groove, and the target is fixed in the clamping groove. After assembly, the slide 300 is placed on the first annular protrusion for isolating the intra-abdominal environment from the external environment, avoiding infection of the experimental animal, and also providing an observation imaging window. As shown in fig. 5, the imaging device is used for fixing the target intestinal tissue in the clamping groove, so that the loss of an observation site caused by intestinal peristalsis during long-time imaging can be avoided, and the fixed-point long-time imaging research on the target intestinal section is facilitated.
In a specific embodiment, the clamping groove and the abdominal window ring are made of materials with good biocompatibility, such as titanium alloy.
In an embodiment, as shown in fig. 1 and2, the edge of the card 120 and the edge of the notch 231 are in a circular arc shape that fits with each other, so as to ensure that the card 120 can pass through the notch 231 to perform the fitting groove.
In one embodiment, as shown in fig. 1, the two side walls of the slot are symmetrical and have the same width (such as the length in the arrow direction in the a diagram) as the width of the bottom surface, and the width of the fixing pile 110 is smaller than the width of the bottom surface, i.e. on one side of the slot, the two U-shaped slot side walls 130 are flush with the edges of the fixing device 110, and on the other side of the slot, the fixing device 110 is retracted inward relative to the U-shaped slot side walls 130 so as to increase the exposed area of the object.
In one embodiment, as shown in fig. 2, the abdominal window ring 200 further includes symmetrical fixing pins 250 extending upward from the annular window 210, and each fixing pin 250 extends over the front surface of the annular window 210, i.e., the fixing pins 250 protrude above the front surface of the annular window 210. In fig. 2, the number of the fixing pins 250 is two, but the present invention is not limited thereto. Further, as shown in fig. 2, the orthographic projections of the two symmetrically disposed fixing bolts 250 and the two symmetrically disposed notches are located on the same diameter line of the annular window, when the abdominal window ring 200 is stitched, the rear structure cannot be observed, at this time, the positions of the notches 231 can be identified by the fixing bolts 250, and then the clamping groove 100 is accurately embedded in the assembly groove of the abdominal window ring 200.
In one embodiment, as shown in fig. 4, the imaging device further includes a snap spring 400 for use with the abdominal ring 200. The clamping spring 400 has a first end and a second end, the outer edge of the clamping spring is in a circular arc shape with an angle larger than 180 degrees, and the clamping spring 400 is matched with the inner wall of the annular window 210, namely, the clamping spring 400 can be placed in the annular window 210 and is tightly contacted with the inner wall of the annular window 210 to be fixed. In a specific operation, the slide 300 is placed on the first annular protrusion 220, then the two ends of the clamp spring 400 are clamped by forceps to enable the clamp spring 400 to elastically deform to slightly reduce the outer diameter, and then the slide is placed in the annular window 210, and after the forceps are loosened, friction force is generated between the clamp spring 400 and the inner side wall of the annular window to fix the slide 300 between the clamp spring 400 and the first annular protrusion 220. Further, as shown in fig. 4, the width of the first end and the second end of the clip 400 is greater than the width of the middle circular arc connecting portion, and each end is provided with a clamping through hole, so that the clamping tool extends into the clamping tool.
In one embodiment, as shown in fig. 6, the imaging device further includes a fixing plate 500 for use with the abdominal window ring 200, the fixing plate 500 including a perspective through hole 510 and a positioning hole 520 adapted to the fixing plug 250. As shown in fig. 7, after the abdominal ring 200 and the card slot 100 are assembled to the subject, an assembly structure S12 is formed, the fixing plug 250 is inserted into the positioning hole 520 to be fixed with the fixing plate 500, and after the fixing plug 250 is inserted into the positioning hole 520, the abdominal ring 200 is aligned with the perspective through hole 510 and the internal image of the abdominal ring 200 is observed through the perspective through hole 510. Specifically, the fixing plate 500 further includes a fixing screw hole 530, and is fixed with the bread board 600 on the three-dimensional translation stage 700 through the fixing screw hole, so that the influence of motion artifacts caused by heartbeat and respiration on the imaging result is reduced through fixing the whole device, and thus the negative influence of the shake caused by the respiration and heartbeat of the abdominal viscera of the animal on the maintenance of the imaging focal plane stability can be prevented.
In a specific embodiment, the dimensions of the above related structures are illustrated.
Wherein the annular window 210 has an outer diameter of 17mm and a thickness of 2.4mm. After the symmetrical fixing plug 250 is designed, the overall diameter thereof becomes 20mm and the thickness becomes 4.9mm. The front opening diameter of the annular window 210 is 12.4mm. The front opening diameter of the annular window 210, the outer diameter of the snap spring 400, and the outer diameter of the first annular protrusion 220 are the same. For example, the first annular protrusion 220 may have an outer diameter of 12.4mm and a fenestration diameter (inner diameter) of 11mm, and the structure is used to fit a standard glass having a diameter of 12mm and a snap spring having an outer diameter of 12.4mm. Because the first annular protrusion 220 is designed to be 1 to 1.2mm deep from the front face of the annular window in consideration of the thickness of the clamp spring and the thickness of the slide. The structure can be effectively and firmly matched with the slide and the clamp spring, so that the slide can be conveniently replaced and taken down for imaging in the subsequent imaging process. The outer diameter of the second annular protrusion 230 is 12.8mm, which is larger than the outer diameter of the first annular protrusion 220, and the gap width of the second annular protrusion 230 is 5.2mm. Considering the size structure of the clamping groove, in order to be convenient for installing the clamping groove in a matched manner in the operation process and fixing intestinal tissues, a circular assembly groove is designed, the width of the assembly groove is 0.4mm, and the diameter of the assembly groove is 12.8mm. The structure can ensure that the clamping groove is quickly installed in the operation process, and the clamping groove cannot fall off due to peristalsis of intestinal tissues and the like in the subsequent imaging process. In order to prevent experimental animals from biting the window during survival and to facilitate the fixation of the window during imaging, the fixation peg 250 is in the shape of a circular arc having two parts integrally formed, the first part is connected with the annular window 210, the inner diameter of which is 17mm, the outer diameter of which is 18mm, which is lower than 0.1mm of the window surface, the second part has an inner diameter of 18mm, an outer diameter of 20mm, an arc of 18.90 degrees, and a total height of 3.2mm, and the protruding window surface has a height of 2.5 mm. The effect of this junction motion artifact on the imaging results. The suture groove 240 is sutured with the wound of the experimental animal, and in order to ensure the rigidity of the window and reduce the size of the wound, the groove depth of the structure is designed to be 1.8mm, and the groove width is designed to be 1.3mm. Skin of the wound is sutured and fixed in the groove through the non-absorptive operation suture, the area of the wound is reduced through the design of the inner groove, the size of the sutured wound is a circle with the diameter of 6.7mm, the wound is prevented from being exposed, and the recovery and survival of experimental animals are facilitated. The design of the structure can fix the device and experimental animals of the whole invention, and can ensure that the survival time of the experimental animals is longer than 4 weeks.
The clamping groove 100 is made of titanium alloy, has a circular arc-shaped structure with a width of 5mm and a radius of 6mm in top view, and has a thickness of 0.4mm and is just embedded in the assembly groove of the abdominal window ring. U-shaped groove depth 2.9mm, the height of the fixed pile 110 is slightly lower than the groove depth, and is 2.7mm high, the structure is 0.6mm thick, the groove with the width of 9mm is divided into two parts, and the whole part is subjected to fillet treatment, so that intestinal tissues are prevented from being damaged, and the survival of experimental animals is prevented from being influenced. Further, the U-shaped groove side wall 130 of the clamping groove 100 is also in an arc shape, the outer diameter of the U-shaped groove side wall is 5.5mm, the height of the U-shaped groove side wall is 3.4mm, two deep grooves are formed between the two side walls and the fixing piles, and the experimental animal is ensured not to influence the expansion and contraction of the fixed intestinal section in the digestion process. After the clamping groove for fixing the intestinal tissue is assembled into the window body, the intestinal tissue can be effectively fixed, and the visual field loss of a research target caused by peristalsis of the intestinal tissue is avoided.
The following describes a method for using the device, which includes the steps of:
Step S1: the abdominal window ring is arranged in the abdominal cavity of the experimental animal through operation, and the window body is fixed through non-absorptive operation suture.
Step S2: the targeted intestinal tissue segment of the imaging study was secured in a clamping groove, which was fitted into the abdominal window ring.
Step S3: and assembling the slide and the clamp spring on the abdominal window ring.
Step S4: the animal-sutured abdominal window ring is assembled into an imaging fixation plate with the final assembly effect shown in fig. 7, and then subjected to optical imaging studies including, but not limited to, confocal scanning imaging, two-photon imaging, and the like.
The device and the surgical instruments in all the steps are sterilized by using sterilizing alcohol or ultraviolet irradiation, and the experimental animals are subjected to peritoneal anesthesia or gas anesthesia in the surgical and imaging processes.
FIG. 8 is a colon tissue structure obtained by optical imaging of C57BL/6 mice (the present description is not limited to mice) at the bulk level using the apparatus and method of the present invention. Imaging using a confocal scanning imaging system, mice were under anesthesia (intraperitoneal or respiratory anesthesia) and enteric neurons were marked by in situ injection. The ganglion, neuron and nerve fiber structure in the enteric nervous system in the intestinal tissue can be clearly distinguished in fig. 8, and the experimental result proves that the device can realize in-vivo level imaging research.
In conclusion, the abdomen window ring and the clamping groove matched with the abdomen window ring are designed. After the abdominal window ring is sutured with the study object through the suture groove, the clamping groove is stretched into the interior through the abdominal window ring window by using tweezers, the fixing piles of the clamping groove are hooked on the target object (intestinal tract), then two cards on the clamping groove are stretched into the assembly groove through two notches of the second annular bulge and rotated for a certain angle, the cards are embedded in the assembly groove, the target object is fixed in the clamping groove, the original study target is prevented from being lost due to intestinal peristalsis, and the fixed-point long-time imaging study on the target intestinal segment is facilitated.
It will be readily appreciated by those skilled in the art that the foregoing description is merely a preferred embodiment of the invention and is not intended to limit the invention, but any modifications, equivalents, improvements or alternatives falling within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (10)
1. An in-vivo optical imaging device suitable for animal intestinal tissue, comprising a matched abdominal window ring and a clamping groove, wherein:
the clamping groove comprises an integrally formed U-shaped groove body and fixing piles, wherein the U-shaped groove body is formed by surrounding a bottom surface and two opposite side walls, the fixing piles extend from the bottom surface of the U-shaped groove body to the direction of an opening at the top, and the two side walls of the U-shaped groove body are respectively provided with a clamping piece which extends along the direction deviating from the U-shaped groove body;
The abdomen window ring includes annular window form and extends the first annular bulge and the second annular bulge that form from the different degree of depth positions of annular window inner side wall to the ring center, first annular bulge with the second annular bulge is followed the direction of the front directional back of annular window form sets up side by side and has the clearance in order to form the assembly groove, first annular bulge is apart from the positive degree of depth of annular window form is greater than 0, the protruding inboard breach that sets up of second annular, the breach with card looks adaptation is so that the card of draw-in groove passes through the protruding breach of second annular is inlayed in the assembly groove, the outer lateral wall of annular window is formed with the seam groove of inwards sunkening.
2. The in-vivo optical imaging device for intestinal tissue of an animal of claim 1, wherein an outer edge of the second annular projection and an outer edge of the first annular projection are axially aligned along an annular window, an inner diameter of the annular window between the first annular projection and the second annular projection being greater than an inner diameter of the annular window between the first annular projection and a front face of the annular window.
3. The in-vivo optical imaging device for animal intestinal tissue of claim 1 wherein said peg is located in a middle position of the bottom surface of the U-shaped channel, the width of said peg being less than the width of the bottom surface of the U-shaped channel.
4. The in-vivo optical imaging device for animal intestinal tissue of claim 1 wherein the edges of the card are arcuate and the edges of the notch are arcuate to fit the card.
5. The in-vivo optical imaging device for intestinal tissue of an animal of claim 1 wherein said abdominal window ring further comprises symmetrically disposed securing pegs extending upwardly from said annular window, each securing peg extending a height above the front face of said annular window.
6. The in-vivo optical imaging device for intestinal tissue of an animal of claim 5 wherein the orthographic projections of the symmetrically disposed securing pegs and notches are all located on a straight line of the same diameter of the annular window.
7. The in-vivo optical imaging device for animal intestinal tissue of claim 1, further comprising a snap spring for use with said abdominal window ring, said snap spring having a first end and a second end and having an outer edge in the shape of a circular arc with an angle greater than 180 °, said snap spring being adapted to the inner side of said annular window and securing the slide between the snap spring and the first annular protrusion by friction with the inner wall of the annular window.
8. The in-vivo optical imaging device for animal intestinal tissue of claim 7 wherein the width of the first end and the second end of the snap spring is greater than the width of the intermediate circular arc shaped connecting portion and each end is provided with a clamping through hole.
9. An in-vivo optical imaging device for use with animal intestinal tissue as defined in claim 5 further comprising a fixation plate for use with said abdominal cavity ring, said fixation plate comprising a see-through hole and a positioning hole adapted for said fixation plug for insertion into the positioning hole for fixation with said fixation plate, the abdominal cavity ring being aligned with said see-through hole and viewing an image of the interior of the abdominal cavity ring through the see-through hole after the fixation plug is inserted into the positioning hole.
10. The in-vivo optical imaging device for animal intestinal tissue of claim 1 wherein the curved locations of the clamping groove are smoothly curved.
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| CN202210431983.9A CN114869230B (en) | 2022-04-22 | 2022-04-22 | In-vivo optical imaging device suitable for animal intestinal tissue |
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| CN202210431983.9A CN114869230B (en) | 2022-04-22 | 2022-04-22 | In-vivo optical imaging device suitable for animal intestinal tissue |
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| CA2375168A1 (en) * | 1999-06-09 | 2000-12-14 | Medispectra, Inc. | An optical probe accessory device for use in in vivo diagnostic procedures |
| CN112022090A (en) * | 2019-06-03 | 2020-12-04 | 华中科技大学 | Photoacoustic/fluorescent bimodal imaging method and device suitable for animal abdominal cavity internal organs |
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| KR101767339B1 (en) * | 2016-05-26 | 2017-08-10 | 한국과학기술원 | Window apparatus and method for in vivo microscopic imaging of pancreatic tissue for obtaining image using the same |
| KR20190010305A (en) * | 2017-07-21 | 2019-01-30 | 삼성전자주식회사 | Optical sensor device with optical structure for noise reduction and electronic device including the same |
| CN215305786U (en) * | 2021-01-28 | 2021-12-28 | 首都医科大学宣武医院 | Window assembly applied to animal experiment living body imaging |
| CN113509286A (en) * | 2021-06-25 | 2021-10-19 | 南京景瑞康分子医药科技有限公司 | Animal abdominal window manufacturing method suitable for optical in-vivo organ imaging |
| CN113907907A (en) * | 2021-09-30 | 2022-01-11 | 广东省科学院健康医学研究所 | Mouse dorsal spine skin pleat window model system and microcirculation constant temperature control method |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2375168A1 (en) * | 1999-06-09 | 2000-12-14 | Medispectra, Inc. | An optical probe accessory device for use in in vivo diagnostic procedures |
| CN112022090A (en) * | 2019-06-03 | 2020-12-04 | 华中科技大学 | Photoacoustic/fluorescent bimodal imaging method and device suitable for animal abdominal cavity internal organs |
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