CN114846946A - Method for improving germination rate of peucedanum praeruptorum dunn seeds - Google Patents
Method for improving germination rate of peucedanum praeruptorum dunn seeds Download PDFInfo
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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Abstract
The invention discloses a method for providing the germination rate of peucedanum praeruptorum dunn seeds, which comprises the steps of collecting well-developed mature and plump peucedanum praeruptorum dunn fruits, placing the fruits indoors, and kneading the fruits to remove impurities for later use after the seeds are matured; storing the cleaned seeds at 4-8 ℃ in dark place and at low temperature; washing the seeds with distilled water for 2-3 times before sowing, then sterilizing with 3% sodium hypochlorite solution for 15min, and then soaking in mixed solution of gibberellin, compound sodium nitrophenolate and cytokinin for 12 h; storing in sand at low temperature for 2 weeks, taking out the seeds, cleaning, and sowing on germination accelerating matrix. The result shows that the method for improving the germination rate of the peucedanum praeruptorum dunn seeds provided by the invention enables the germination rate of the peucedanum praeruptorum dunn seeds which are stored for 8 months after being harvested to reach more than 70% when the peucedanum praeruptorum dunn seeds are sown for 28 days, improves the germination rate, shortens the germination time and facilitates large-scale artificial cultivation.
Description
Technical Field
The invention belongs to the technical field of plant propagation, and particularly relates to a method for improving the germination rate of peucedanum praeruptorum dunn seeds.
Background
Peucedanum praeruptorum Dunn is a perennial herb of Peucedanum (Peucedanum) of Peucedanum of Umbelliferae. The root is used as the medicine, has the efficacies of dispelling wind and clearing heat, descending qi and reducing phlegm and the like, and is mainly used for treating diseases of tracheitis and bronchitis, such as wind-heat cough, excessive phlegm and asthma. Peucedanum praeruptorum dunn is a traditional bulk drug in China and is also one of 'new Zhejiang eight flavors' in Zhejiang province. Since the early peucedanum praeruptorum is mainly collected by the wild, the wild resources are gradually exhausted along with the expansion of market demands. In recent years, the planting area is gradually increased, and the producing areas are distributed in a plurality of provinces and cities in China. Seeds are the main route for propagation of peucedanum praeruptorum dunn. In the production practice of pharmacotherapy, it is found that peucedanum praeruptorum dunn seeds are easily affected by environmental conditions and climatic factors, and the phenomena of low germination rate, irregular germination or prolonged germination period and the like generally exist, so that the bred plants have different growth vigors, small plant quantity, long production period and low emergence rate, and the large-scale production of the peucedanum praeruptorum dunn seeds is limited.
Disclosure of Invention
The invention aims to provide a method for improving the germination rate of peucedanum praeruptorum dunn seeds aiming at the defects of the existing peucedanum praeruptorum dunn seed propagation technology, which can obviously improve the germination rate of peucedanum praeruptorum dunn seeds and is convenient for large-scale artificial planting.
The purpose of the invention is realized by the following technical scheme:
a method for improving the germination rate of peucedanum praeruptorum dunn seeds comprises the steps of collecting well-developed and plump peucedanum praeruptorum dunn fruits, placing the praeruptorum dunn fruits indoors for after-ripening, rubbing the seeds to remove impurities, keeping the seeds clean at 4-8 ℃ in the dark, disinfecting the seeds with a 3% sodium hypochlorite solution before sowing, then soaking the seeds in a mixed solution of gibberellin, sodium nitrophenolate and cytokinin for 12 hours, and sowing the seeds on a germination accelerating medium for germination after sand storage at 0-4 ℃ for 2 weeks.
Further, the well-developed and full peucedanum praeruptorum dunn fruits are collected, placed indoors, are kneaded after the seeds are ripened, are cleaned, and are stored in a dark place at the temperature of 4-8 ℃, and the method specifically comprises the following steps:
selecting a main inflorescence of the peucedanum praeruptorum dunn when the color of the peucedanum praeruptorum dunn fruits is changed into yellow white in 9-10 months, cutting off the fruits which do not have plant diseases and insect pests and grow into strong plants together with pedicels, filling the fruits into a breathable woven bag, placing the woven bag in a room, stacking for 3-5 days for after-ripening, wherein the stacking height is not more than 25cm, irregularly turning over the fruits during stacking to ensure that the fruits are completely ripe, kneading the fruits after the after-ripening is completely finished, removing impurities, and airing; and filling the aired seeds into a woven bag, and storing the seeds in a dark place at the temperature of 4-8 ℃.
Further, before sowing, the seeds are disinfected by a 3% sodium hypochlorite solution and then soaked in a mixed solution of gibberellin, compound sodium nitrophenolate and cytokinin for 12 hours, and the method specifically comprises the following steps:
washing the selected seeds with distilled water for 2-3 times, soaking with 3% sodium hypochlorite solution for 15min, taking out, washing with distilled water for 3-5 times, and draining; soaking the drained seeds in a mixed solution containing 100mg/L of gibberellin, 10mg/L of compound sodium nitrophenolate and 10mg/L of cytokinin for 12h, draining, and then filling into a breathable cloth bag.
Further, the sand storage at 0-4 ℃ for 2 weeks specifically comprises:
spreading fine sand 5-10cm away from the bottom of the container, spreading the cloth bag containing seeds on the fine sand, covering with fine sand 3-5cm thick, and storing in sand at 0-4 deg.C for 2 weeks.
Further, the sowing on the germination accelerating substrate for germination specifically comprises: taking out the seeds after sand storage, cleaning the seeds with distilled water, sowing the seeds on a germination accelerating substrate, and covering the germination accelerating substrate with a thickness of 0.3 cm; the germination accelerating matrix is flower soil, perlite and vermiculite which are uniformly mixed according to the volume ratio of 3:1: 1; the germination condition is 20-25 deg.C, humidity is 70% -80%, and the illumination condition is 12h illumination/12 h darkness.
The invention has the beneficial effects that: the method adopts the method of seed soaking by using the exogenous reagent and low-temperature sand storage treatment, improves the germination rate of the peucedanum praeruptorum dunn, improves the uniformity of seedling emergence, ensures uniform seedling emergence size, is convenient for artificial large-scale planting, and has simple method and easy popularization.
Detailed Description
The invention provides a method for improving the germination rate of peucedanum praeruptorum dunn seeds, which improves the germination rate of peucedanum praeruptorum dunn seeds by sterilizing the peucedanum praeruptorum dunn seeds once, soaking the seeds in exogenous reagents and storing the seeds in sand at a low temperature, and specifically comprises the following steps:
1) seed collection: the fruits of peucedanum praeruptorum dunn are ripe in 9-10 months generally, when the color of the fruits is changed into yellow white, the main inflorescence of the peucedanum praeruptorum dunn which has no plant diseases and insect pests and can grow strong plants is selected, the fruits and the pedicels are sheared together, the fruits are placed in an air-permeable woven bag and stacked for 3-5 days for after-ripening, the stacking height is not more than 25cm, the fruits are turned over irregularly during stacking to ensure that the fruits are completely ripe, and the fruits are kneaded after the after-ripening is complete (seeds are full and brown), impurities are removed, and the fruits are dried for later use.
2) And (4) low-temperature preservation: and filling the cleaned seeds into a woven bag, and storing the seeds in a dark place at the temperature of 4-8 ℃.
3) Disinfecting and seed soaking: washing the selected seeds with distilled water for 2-3 times, soaking with 3% sodium hypochlorite solution for 15min, taking out, washing with distilled water for 3-5 times, and draining; soaking the drained seeds in Gibberellin (GA) 3 100mg/L), compound sodium nitrophenolate (10mg/L) and cytokinin (6-BA 10mg/L) for 12h, draining, and filling into a breathable cloth bag.
4) And (3) low-temperature sand storage: spreading fine sand with thickness of 5-10cm at the bottom of the container, spreading the cloth bag containing seeds on the fine sand, covering the fine sand with thickness of 3-5cm, and storing in sand at low temperature of 0-4 deg.C for 2 weeks.
5) And (3) germination treatment: taking out the seeds, cleaning the seeds with distilled water, sowing the seeds on a germination accelerating substrate, and covering the germination accelerating substrate with a thickness of 0.3 cm. The germination accelerating matrix is flower soil, perlite and vermiculite which are uniformly mixed according to the volume ratio of 3:1: 1; the germination condition is 20-25 deg.C, humidity is 70% -80%, and the illumination condition is 12h illumination/12 h darkness.
The objects and effects of the present invention will become more apparent by describing in detail specific embodiments of the present invention. The reagents used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores. The quantitative data in the following examples were set up in triplicate and the results averaged.
Example 1:
a method for improving the germination rate of peucedanum praeruptorum dunn seeds comprises the following steps:
1) seed collection: the fruits of the peucedanum praeruptorum dunn are ripe in 9-10 months generally, when the color of the fruits is changed into yellow white, the main inflorescence of the peucedanum praeruptorum dunn is selected, the fruits which do not have diseases and insect pests and grow strong plants are cut off together with pedicels, the fruits are put into a breathable woven bag and are placed in a room to be stacked for 3-5 days for after-ripening, the stacking height is not more than 25cm, the fruits are turned over irregularly during stacking to ensure that the fruits are completely ripe, and the fruits are kneaded after the after-ripening is complete (seeds are full and brown), impurities are removed, and the fruits are dried for standby.
2) And (4) low-temperature preservation: and putting the cleaned seeds into a woven bag, and storing for 8 months in a dark place at the temperature of 4-8 ℃.
3) And (3) disinfection treatment: soaking the selected seeds in 3% sodium hypochlorite solution for 10min, taking out, washing with distilled water for 3 times, and draining.
4) And (3) low-temperature sand storage: spreading fine sand with thickness of 5-10cm at the bottom of the container, spreading the cloth bag containing seeds on the fine sand, covering the fine sand with thickness of 3-5cm, and storing in sand at low temperature of 0-4 deg.C for 2 weeks.
5) And (3) germination treatment: taking out the seeds, cleaning the seeds with distilled water, sowing the seeds on a germination accelerating substrate, and covering the germination accelerating substrate with a thickness of 0.3 cm. The germination accelerating matrix is flower soil, perlite and vermiculite which are uniformly mixed according to the volume ratio of 3:1: 1; the germination condition is 20-25 deg.C, humidity is 70% -80%, and the illumination condition is 12h illumination/12 h darkness.
Comparative example 1:
the invention provides a method for germinating peucedanum praeruptorum dunn seeds, which has the specific process similar to that of example 1, and is characterized in that the seeds are not stored at a low temperature of 4-8 ℃ in a dark place after being collected, but are stored in a shade place at normal temperature for 8 months. The temperature, humidity and illumination control of the subsequent treatment and the seedling and germination acceleration process are the same as those of the embodiment 1.
Example 2:
a method for improving the germination rate of peucedanum praeruptorum dunn seeds comprises the following steps:
1) seed collection: the fruits of the peucedanum praeruptorum dunn are ripe in 9-10 months generally, when the color of the fruits is changed into yellow white, the main inflorescence of the peucedanum praeruptorum dunn is selected, the fruits which do not have diseases and insect pests and grow strong plants are cut off together with pedicels, the fruits are put into a breathable woven bag and are placed in a room to be stacked for 3-5 days for after-ripening, the stacking height is not more than 25cm, the fruits are turned over irregularly during stacking to ensure that the fruits are completely ripe, and the fruits are kneaded after the after-ripening is complete (seeds are full and brown), impurities are removed, and the fruits are dried for standby.
2) And (4) low-temperature preservation: and putting the cleaned seeds into a woven bag, and storing for 8 months in a dark place at the temperature of 4-8 ℃.
3) Disinfecting and seed soaking: washing the selected seeds with distilled water for 2-3 times, soaking with 3% sodium hypochlorite solution for 15min, taking out, washing with distilled water for 3-5 times, and draining; the drained seeds are soaked in sterile water 12, drained and then put into a breathable cloth bag.
4) And (3) low-temperature sand storage: spreading fine sand with thickness of 5-10cm at the bottom of the container, spreading the cloth bag containing seeds on the fine sand, covering the fine sand with thickness of 3-5cm, and storing in sand at low temperature of 0-4 deg.C for 2 weeks.
5) And (3) germination treatment: taking out the seeds, cleaning the seeds with distilled water, sowing the seeds on a germination accelerating substrate, and covering the germination accelerating substrate with a thickness of 0.3 cm. The germination accelerating matrix is flower soil, perlite and vermiculite which are uniformly mixed according to the volume ratio of 3:1: 1; the germination condition is 20-25 deg.C, humidity is 70% -80%, and the illumination condition is 12h illumination/12 h darkness.
Comparative example 2:
the invention provides a method for germinating peucedanum praeruptorum dunn seeds, which has the specific process similar to that of example 2, and is different from the specific process that the seeds are directly sowed on a germination accelerating substrate for germination treatment after being disinfected and soaked. The temperature, humidity and illumination control during the seedling growing and germination accelerating process are the same as those in the embodiment 2.
Example 3:
a method for improving the germination rate of peucedanum praeruptorum dunn seeds comprises the following steps:
1) seed collection: the fruits of peucedanum praeruptorum dunn are ripe in 9-10 months generally, when the color of the fruits is changed into yellow white, the main inflorescence of the peucedanum praeruptorum dunn which has no plant diseases and insect pests and can grow strong plants is selected, the fruits and the pedicels are sheared together, the fruits are placed in an air-permeable woven bag and stacked for 3-5 days for after-ripening, the stacking height is not more than 25cm, the fruits are turned over irregularly during stacking to ensure that the fruits are completely ripe, and the fruits are kneaded after the after-ripening is complete (seeds are full and brown), impurities are removed, and the fruits are dried for later use.
2) And (4) low-temperature preservation: and putting the cleaned seeds into a woven bag, and storing for 8 months in a dark place at the temperature of 4-8 ℃.
3) Disinfecting and seed soaking: washing the selected seeds with distilled water for 2-3 times, soaking with 3% sodium hypochlorite solution for 15min, taking out, washing with distilled water for 3-5 times, and draining; soaking the drained seeds in mixed solution of gibberellin (GA3100mg/L), compound sodium nitrophenolate (10mg/L) and cytokinin (6-BA 10mg/L) for 12h, draining, and packaging in an air-permeable cloth bag.
4) And (3) low-temperature sand storage: spreading fine sand with thickness of 5-10cm at the bottom of the container, spreading the cloth bag containing seeds on the fine sand, covering the fine sand with thickness of 3-5cm, and storing in sand at low temperature of 0-4 deg.C for 2 weeks.
5) And (3) germination treatment: taking out the seeds, cleaning the seeds with distilled water, sowing the seeds on a germination accelerating substrate, and covering the germination accelerating substrate with a thickness of 0.3 cm. The germination accelerating matrix is flower soil, perlite and vermiculite which are uniformly mixed according to the volume ratio of 3:1: 1; the germination condition is 20-25 deg.C, humidity is 70% -80%, and the illumination condition is 12h illumination/12 h darkness.
Comparative example 3:
the comparative example of the present invention provides a method for germinating peucedanum praeruptorum dunn seeds, which is similar to example 3, except that the seeds are soaked in gibberellin (GA3100mg/L) solution for 12h after being sterilized and drained, and then are put into a breathable cloth bag after being drained. The temperature, humidity and illumination control during the seedling growing and germination accelerating process are the same as those in the embodiment 3.
Comparative example 4:
the invention provides a method for germinating peucedanum praeruptorum dunn seeds, which is similar to the embodiment 3 in specific process, and only comprises the steps of disinfecting, draining, soaking the seeds in a compound sodium nitrophenolate (10mg/L) solution for 12 hours, draining, and filling into a breathable cloth bag. The temperature, humidity and illumination control during the seedling growing and germination accelerating process are the same as those in the embodiment 3.
Comparative example 5:
the invention provides a method for germinating peucedanum praeruptorum dunn seeds, which is similar to the embodiment 3 in specific process, and only comprises the steps of soaking the seeds in cytokinin (6-BA 10mg/L) solution for 12 hours after disinfection and draining, and putting the seeds into a breathable cloth bag after draining. The temperature, humidity and illumination control during the seedling growing and germination accelerating process are the same as those in the embodiment 3.
Germination of the seeds was recorded every 48h after sowing until 28 days were observed, the results of which are shown in table 1. Seed germination is marked by radicle extension of the seed coat (white). The germination percentage calculation formula is as follows:
germination percentage (%) - (total number of finally germinated seeds/total number of test seeds) × 100%
TABLE 1
As can be seen from the results in table 1, in example 1, compared with comparative example 1, the temperature condition for storage has a significant effect on the germination of the peucedanum praeruptorum dunn seeds, if the storage time is longer, the seeds need to be stored at 4-8 ℃ in a dark place, otherwise the seeds cannot germinate due to the loss of vitality; example 2 compared with comparative example 2, the germination rate of the peucedanum praeruptorum dunn seeds can be improved to a certain extent through low-temperature sand storage; example 3 compared with comparative example 3, comparative example 4 and comparative example 5, the germination of peucedanum praeruptorum dunn seeds soaked by gibberellin, sodium polynitrate and cytokinin solutions alone can be promoted, wherein the germination rate of the gibberellin-treated seeds is 65% which is higher than that of the seeds treated by sodium polynitrate and cytokinin alone, and the germination rate of the seeds can be improved to 73% more remarkably by the gibberellin (GA3100mg/L), sodium polynitrophenate (10mg/L) and cytokinin (6-BA 10mg/L) mixed solution, and the germination time is advanced to 5 days which is better than that of the other three control groups.
The foregoing is a more detailed description of the invention in connection with specific preferred embodiments and it is not intended that the invention be limited to these specific details. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. A method for improving germination rate of peucedanum praeruptorum dunn seeds is characterized in that mature and plump peucedanum praeruptorum dunn fruits are collected and placed indoors, after the seeds are ripened, the seeds are rubbed to remove impurities, the seeds are kept in a dark place at 4-8 ℃, the seeds are disinfected by a 3% sodium hypochlorite solution before sowing, then the seeds are soaked in a mixed solution of gibberellin, compound sodium nitrophenolate and cytokinin for 12 hours, and the seeds are sown on a germination accelerating medium for germination after being stored in sand at 0-4 ℃ for 2 weeks.
2. The method for improving the germination rate of the peucedanum praeruptorum dunn seeds of claim 1, wherein the well-developed and plump peucedanum praeruptorum dunn fruits are collected, placed indoors, are subjected to after-ripening, are kneaded to remove impurities, are cleaned, and are stored in a dark place at 4-8 ℃, and specifically:
selecting a main inflorescence of the peucedanum praeruptorum dunn when the color of the peucedanum praeruptorum dunn fruits is changed into yellow white in 9-10 months, cutting off the fruits which do not have plant diseases and insect pests and grow into strong plants together with pedicels, filling the fruits into a breathable woven bag, placing the woven bag in a room, stacking for 3-5 days for after-ripening, wherein the stacking height is not more than 25cm, irregularly turning over the fruits during stacking to ensure that the fruits are completely ripe, kneading the fruits after the after-ripening is completely finished, removing impurities, and airing; and filling the aired seeds into a woven bag, and storing the seeds in a dark place at the temperature of 4-8 ℃.
3. The method for improving the germination rate of the peucedanum praeruptorum dunn seeds of claim 1, wherein the seeds are disinfected by a 3% sodium hypochlorite solution before sowing and then soaked in a mixed solution of gibberellin, compound sodium nitrophenolate and cytokinin for 12 hours, and specifically comprises the following steps:
washing the selected seeds with distilled water for 2-3 times, soaking with 3% sodium hypochlorite solution for 15min, taking out, washing with distilled water for 3-5 times, and draining; the drained seeds are soaked in a mixed solution containing 100mg/L of gibberellin, 10mg/L of compound sodium nitrophenolate and 10mg/L of cytokinin for 12 hours.
4. The method for improving the germination rate of the peucedanum praeruptorum dunn seeds of claim 1, wherein the sand storage at 0-4 ℃ for 2 weeks is specifically as follows:
spreading fine sand 5-10cm away from the bottom of the container, spreading the cloth bag containing seeds on the fine sand, covering with fine sand 3-5cm thick, and storing in sand at 0-4 deg.C for 2 weeks.
5. The method for improving the germination rate of the peucedanum praeruptorum dunn seeds as claimed in claim 1, wherein the seeds are sowed on a germination accelerating substrate for germination, and specifically the germination accelerating substrate comprises the following steps: taking out the seeds after sand storage, cleaning the seeds with distilled water, sowing the seeds on a germination accelerating substrate, and covering the germination accelerating substrate with a thickness of 0.3 cm; the germination accelerating matrix is flower soil, perlite and vermiculite which are uniformly mixed according to the volume ratio of 3:1: 1; the germination condition is 20-25 deg.C, humidity is 70% -80%, and the illumination condition is 12h illumination/12 h darkness.
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| CN111108842A (en) * | 2019-12-12 | 2020-05-08 | 贵州金草海药材发展有限公司 | Peucedanum praeruptorum seed treatment method |
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