CN114774266A - Multi-channel integrated sample adding device and method thereof - Google Patents

Abstract

The invention discloses a multi-channel integrated sample adding device and a method thereof, and the multi-channel integrated sample adding device comprises a base, a Z-direction driving device, a Z-direction guide rail, a Z-direction sliding block and a sample adding mechanism, wherein the sample adding mechanism can move up and down along the Z-direction guide rail under the driving of the Z-direction driving device, the sample adding mechanism comprises a first motor, a piston assembly and a gun arranging module, the piston assembly is connected with the output end of the first motor, the gun arranging module is arranged at the lower end of the piston assembly, the piston is communicated and connected with an adapter, and the multi-channel integrated sample adding device solves the problems of fewer parallel channels and low efficiency in the prior art through the integral matching of the driving device, the piston assembly and the gun arranging module, and solves the problems of larger size, low integration level and low flexibility of multi-channel sample adding equipment in the prior art Imbibition, notes liquid, the operation of taking off the needle to can realize real-time detection, very big improvement work efficiency.

Description

Multi-channel integrated sample adding device and method thereof

Technical Field

The invention relates to the technical field of medical instruments, in particular to a multi-channel integrated sample adding device and a method thereof.

Background

The full-automatic nucleic acid extraction instrument is an instrument integrating functions of sample distribution, reagent distribution, liquid transfer, magnetic attraction, oscillation mixing and the like required by nucleic acid extraction and purification tests based on a magnetic bead separation principle methodology, generally uses a 96-hole deep-hole plate as a test carrier, and can realize automatic extraction and purification of high-flux and high-purity nucleic acid. The full-automatic nucleic acid extractor generally comprises a sample adding arm module, an oscillation module, a sample test tube rack module, a magnetic rack module and the like.

Full-automatic nucleic acid extraction appearance generally adopts disposable application of sample needle to carry out the absorption of liquid and with the liquid distribution of absorption to the reaction plate in to avoid cross contamination, consequently application of sample arm module need have automatic getting needle, imbibition, annotate liquid and automatic needle function of taking off. In the prior art, the sample adding channel is less, the treatment efficiency is not high, and the clinical requirements of large-batch nucleic acid extraction and detection cannot be met. On the other hand, among the prior art, the application of sample module often through setting up a drive module at every passageway to get the needle and take off the needle to the passageway and do control, when the passageway is more, a plurality of passageway application of sample arm modules that this kind of mode was integrated are bulky, make the whole occupation space of full-automatic nucleic acid extraction appearance great, are unfavorable for the miniaturization of equipment.

Therefore, research and improvement on the multi-channel integrated sample adding device and method thereof are needed to solve the problems in the prior art.

Disclosure of Invention

The invention provides a multi-channel integrated sample adding device and a method thereof, aiming at overcoming the defects in the prior art. This multichannel integration application of sample device can realize getting the needle of multichannel, imbibition, annotate liquid and take off the needle operation to operation process is more stable, has improved nucleic acid and has drawed work efficiency, and the occupation space of device is less, does benefit to the miniaturization of equipment.

The invention provides a multi-channel integrated sample adding device, which comprises:

the utility model provides a multichannel integration application of sample device, its characterized in that includes the base, be provided with Z on the base and to drive arrangement, Z to guide rail, set up Z to slider, application of sample mechanism on the Z is to the guide rail.

Furthermore, the Z-direction driving device can drive the Z-direction slider on the Z-direction guide rail to enable the Z-direction slider to move up and down on the Z-direction guide rail, the sample adding mechanism is arranged on the Z-direction slider on the Z-direction guide rail, and the sample adding mechanism can extend the Z-direction guide rail to move up and down under the driving of the Z-direction driving device.

Furthermore, the sample adding mechanism comprises a first motor, a piston assembly and a gun arranging module, the piston assembly comprises pistons the number of which is the same as that of the channels, the gun arranging module comprises adapters the number of which is the same as that of the channels, the piston assembly is connected to the output end of the first motor, the gun arranging module is arranged at the lower end of the piston assembly, and the pistons are communicated with the adapters.

Further, the number of the channels is eight.

Furthermore, the piston assembly comprises a piston connecting plate, the pistons are arranged in front and back rows, the first row of pistons and the second row of pistons are arranged at intervals in a staggered mode, and the upper ends of the pistons are connected with the piston connecting plate.

Further, the rifle arranging module still includes the rifle arranging cavity, the upper portion of the rifle arranging cavity is equipped with piston installation position, the lower part of the rifle arranging cavity is equipped with adapter installation position, piston installation position is two rows of dislocation sets in front and back, adapter installation position sets up in the centre of two rows of piston installation positions, the lower extreme of piston is inserted and is located piston installation position, the upper end of adapter is inserted and is located adapter installation position, be equipped with the air vent in the rifle arranging cavity, the upper end of adapter with the route of gas circuit is realized through the air vent to the lower extreme of piston.

Further, the rifle arranging module further comprises a needle releasing plate, a needle releasing rod and a photoelectric switch detection plate, wherein the needle releasing plate is arranged below the rifle arranging cavity, photoelectric switch separation blade notches are formed in the edges of the front side and the rear side of the needle releasing plate, a plurality of photoelectric switches are arranged on the photoelectric switch detection plate, the photoelectric switch detection plate is arranged above the needle releasing plate, the photoelectric switches correspond to the photoelectric switch separation blade notches, and the two ends of the needle releasing rod are respectively connected with the rifle arranging cavity and the needle releasing plate.

Further, the rifle arranging module still includes the needle cover of taking off the same with passageway quantity, take off the needle cover in on the adapter, and crisscross two rows around setting up in the adapter, take off the needle cover and pass through the anticreep bolt fastening in take off on the faller, the cover is equipped with the needle spring of taking off on the anticreep bolt, the upper portion of taking off the needle cover is equipped with the photoelectric switch separation blade, the photoelectric switch separation blade that the light separation blade can insert on the needle board of taking off cooperates with the photoelectric switch who locates on the photoelectric switch detection board for get the needle and take off the detection that the needle targets in place.

Further, arrange the rifle module and still include atmospheric pressure detection module, atmospheric pressure detection module set up in arrange the left and right sides of rifle cavity to communicate with the inside passageway of piston, be used for detecting the change of imbibition or liquid capacity when annotating the liquid.

Further, the air pressure detection module comprises an air pressure detection plate, a sealing ring, a spring and a spring cover, wherein the sealing ring is arranged between the air pressure detection plate and a channel at the lower end of the piston, and the spring cover are sequentially arranged on the outer side of the air pressure detection plate and used for sealing the air pressure detection plate and the channel inside the piston.

The invention also provides a sample adding method of the multi-channel integrated sample adding device, which comprises the following steps:

a sample adding method of a multi-channel integrated sample adding device is characterized by being used for the multi-channel integrated sample adding device and comprising the following steps:

when the sample adding mechanism moves to the position above the needle taking position, the Z-direction driving device drives the sample adding mechanism to move downwards, and the tail end of the adapter is inserted into an opening of a sample adding needle to perform needle taking action;

when the sample adding mechanism moves to the position above the liquid absorbing position and the sample adding needle on the adapter is driven to move downwards in the Z direction to the position below the liquid surface to be absorbed by the liquid, the first motor drives the piston to move upwards, so that the adapter communicated with the piston performs liquid absorbing work;

the sample adding mechanism moves to a sample adding position, and the first motor drives the piston to move downwards so that the adapter communicated with the piston performs liquid injection work;

the sample adding mechanism moves to a needle releasing area, the first motor drives the piston connecting plate to move downwards, the piston connecting plate extrudes the needle releasing rod to move downwards, the needle releasing rod drives the needle releasing plate to move downwards, and then the needle releasing sleeve is driven to extrude the sample adding needle downwards to realize needle releasing.

Further, the sample adding method also comprises the following steps:

the sample adding needle is pressed against the needle removing sleeve to move upwards, and the photoelectric switch blocking piece on the needle removing sleeve enters the photoelectric switch detection range to carry out needle taking in-place detection;

the needle release plate drives the needle release sleeve to move downwards, and a photoelectric switch blocking piece on the needle release sleeve moves out of the detection range of the photoelectric switch to detect needle release in place;

the first motor drives the piston to move upwards, so that when the adapter communicated with the piston performs liquid suction work, the air pressure detection modules on the left side and the right side of the gun discharging cavity perform air pressure detection;

when the air pressure detection modules on the left side and the right side of the cavity of the discharging gun receive the synchronous increase of the pressure detection signals, a liquid taking detection normal signal is sent out;

when the air pressure detection modules on the left side and the right side of the gun discharging cavity receive the synchronous reduction of the pressure detection signals, normal liquid injection detection signals are sent out;

when the air pressure detection modules on the left side and the right side of the gun discharging cavity receive asynchronous pressure detection signals, liquid taking or liquid injection abnormal signals are sent out.

Compared with the prior art, the multi-channel integrated sample adding device provided by the invention comprises a base, a Z-direction driving device, a Z-direction guide rail, a Z-direction sliding block and a sample adding mechanism, wherein the sample adding mechanism can extend the Z-direction guide rail to move up and down under the driving of the Z-direction driving device, the sample adding mechanism comprises a first motor, a piston assembly and a gun arranging module, the piston assembly is connected with the output end of the first motor, the gun arranging module is arranged at the lower end of the piston assembly, the piston is communicated and connected with an adapter, the multi-channel integrated sample adding device realizes the operations of needle taking, liquid sucking, liquid injecting, needle removing and the like of multiple channels through the integral matching of the driving device, the piston assembly and the gun arranging module, solves the problems of fewer parallel channels and low efficiency in the prior art, and solves the problems of larger size, low integration level and low flexibility of multi-channel sample adding equipment in the prior art, the multichannel integration application of sample device can improve get the needle, imbibition, annotate the continuity of liquid, the operation of taking off the needle, very big improvement work efficiency.

Drawings

In order to more clearly illustrate the embodiments of the present application or technical solutions in the prior art, the drawings required in the embodiments will be briefly described below, it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to these drawings.

Fig. 1 is a schematic structural diagram of a multi-channel integrated sample adding device according to an embodiment of the present invention;

fig. 2 is an exploded view of a multi-channel integrated sample adding device according to an embodiment of the present invention;

FIG. 3 is an exploded view of a sample feeding mechanism according to an embodiment of the present invention;

FIG. 4 is a top view of a chamber of a gun gang in accordance with an embodiment of the invention;

FIG. 5 is a longitudinal sectional view of a chamber of a line gun according to an embodiment of the present invention;

FIG. 6 is a schematic structural diagram of a sample application mechanism according to an embodiment of the present invention;

fig. 7 is a schematic view of a first viewing angle structure of a sample adding mechanism according to an embodiment of the invention;

FIG. 8 is a second perspective structural view of the sample application mechanism according to an embodiment of the present invention;

fig. 9 is a schematic view of a third perspective structure of the sample adding mechanism according to an embodiment of the invention;

FIG. 10 is a top view of a stripper plate according to one embodiment of the invention;

FIG. 11 is a schematic structural diagram of a chamber of the gun bank according to an embodiment of the present invention;

FIG. 12 is a longitudinal cross-sectional view of a chamber of a gun array in accordance with one embodiment of the present invention.

Wherein, 100-multi-channel integrated sample adding device, 110-base, 111-Z direction driving device, 112-Z direction guide rail, 113-Z direction slide block, 120-sample adding mechanism, 121-first motor, 122-motor fixing plate, 123-guide rod, 124-oil seal fixing plate, 125-oil seal, 130-gun module row, 131-adapter, 132-gun cavity row, 133-piston mounting position, 134-adapter mounting position, 135-vent hole, 136-needle removing plate, 137-needle removing rod, 140-piston component, 141-piston, 142-piston connecting plate, 143-liquid-absorbing photoelectric switch baffle plate, 150-photoelectric switch detecting plate, 151-photoelectric switch baffle plate notch, 152-needle removing photoelectric switch, 153-needle removing sleeve, 154-anti-drop bolt, 155-needle-off spring, 156-needle-taking photoelectric switch baffle, 160-air pressure detection module, 161-air pressure detection plate, 162-sealing ring, 163-spring, 164-spring cover, 170-drag chain, 171-gun arrangement outer cover, 172-gun arrangement connecting plate, 173-Z direction fixing plate, 174-Z direction mounting plate, 175-fixing plate, 177-liquid absorption photoelectric switch, 178-Z direction photoelectric switch baffle and 179-Z direction photoelectric switch.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be further described with reference to the accompanying drawings. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.

It should be noted that all directional indicators (such as up, down, left, right, front, back, etc.) in the embodiments of the present invention are only used for explaining the relative position relationship between the components, the motion situation, etc. in a certain posture, and if the certain posture is changed, the directional indicator is changed accordingly.

As shown in fig. 1-2, a multi-channel integrated sample adding device 100 includes a base 110, a Z-direction driving device 111, a Z-direction guide rail 112, a Z-direction slider 113 disposed on the Z-direction guide rail 112, and a sample adding mechanism 120 are disposed on the base 110, the Z-direction driving device 111 can drive the Z-direction slider 113 on the Z-direction guide rail 112 to move the Z-direction slider 113 up and down on the Z-direction guide rail 112, the sample adding mechanism 120 is disposed on the Z-direction slider 113 on the Z-direction guide rail 112, the sample adding mechanism 120 can move up and down along the Z-direction guide rail 112 under the driving of the Z-direction driving device 111, the sample adding mechanism 120 includes a first motor 121, a piston assembly 140, and a gun arranging module 130, the piston assembly 140 includes pistons 141 with the same number as channels, the gun arranging module 130 includes adapters 131 with the same number as channels, the piston assembly 140 is connected to the output end of the first motor 121, the gun arranging module 130 is disposed at the lower end of the piston assembly 140, the piston 141 is connected to the adapter 131.

This multichannel integration application of sample device 100 is through Z to drive arrangement 111, first motor 121, the whole cooperation of piston assembly 140 and calandria module 130 isotructure, realize multichannel get the needle, imbibition, annotate liquid, take off operations such as needle, compare prior art, this multichannel integration application of sample device 100 can improve and get the needle, imbibition, annotate liquid, take off the continuity of needle operation, very big improvement work efficiency, it is less to solve parallel passageway among the prior art, the problem of inefficiency, and multichannel application of sample equipment size is great among the prior art, the integrated level is low, the problem of low flexibility ratio.

As shown in FIGS. 1-2, the multi-channel integrated sample adding device 100 has eight channels. In general, a nucleic acid extractor is used in combination with a 96-well deep-well plate, which is generally 8 rows and 12 columns. This multichannel integration application of sample device 100's 8 passageways correspond with 8 holes in every row of 96 hole deep hole board, can once accomplish the application of sample operation in 8 holes in a line, have improved work efficiency greatly, avoid simultaneously because the poor problem of precision that every row's application of sample caused many times. Of course, the multi-channel integrated sample adding device 100 can be set to 2-7 channels according to the actual experiment requirement, which does not affect the general concept of the present invention.

As shown in fig. 2-3 and 9, the piston assembly 140 includes a piston connecting plate 142, the pistons 141 are arranged in two front and rear rows, the first row of pistons and the second row of pistons are arranged at intervals in a staggered manner, and the upper ends of the pistons 141 are connected to the piston connecting plate 142. The multiple pistons 141 of the multi-channel integrated sample adding device 100 are tightly connected with the gun discharging cavity 132 through the piston connecting plate 142. This multichannel integration application of sample device 100 makes through the up-and-down motion of piston and changes with piston internal gas pressure to realize imbibition and notes liquid operation, for satisfying in the experimentation, the liquid of certain volume of each passageway imbibition, notes liquid, this piston needs to satisfy certain compression volume change. According to the multi-channel integrated sample adding device, the two rows of pistons are arranged, and the first row of pistons and the second row of pistons are arranged at intervals in a staggered mode, so that the pistons can be arranged to have wider outer diameters, and the length of the pistons can be shorter under the condition that the same piston compression volume is met, so that the multi-channel integrated sample adding device 100 is more exquisite in structure, higher in space utilization rate and beneficial to miniaturization of equipment.

As shown in fig. 2 to 5, the gun arraying module 130 further includes a gun arraying cavity 132, a piston mounting position 133 is disposed on an upper portion of the gun arraying cavity 132, an adapter mounting position 134 is disposed on a lower portion of the gun arraying cavity 132, the piston mounting positions 133 are arranged in a front row and a rear row in a staggered manner, the adapter mounting position 134 is disposed in a middle of the two rows of piston mounting positions 133, a lower end of a piston 141 is inserted into the piston mounting position 133, an upper end of an adapter 131 is inserted into the adapter mounting position 134, a vent hole 135 is disposed in the gun arraying cavity 132, and an upper end of the adapter 131 and a lower end of the piston 141 realize a passage of an air passage through the vent hole 135. This multichannel integration application of sample device 100 passes through row rifle cavity 132, connects multichannel piston 141 and adapter 131, and the piston installation position 133 on row rifle cavity 132 upper portion matches with the setting of piston, also is two rows of dislocation settings in front and back, and piston 141 passes through oil blanket 125 and arranges rifle cavity 132 zonulae occludens, and adapter 131 and row rifle cavity 132 lower part adapter installation position 134 looks zonulae occludens, and adapter installation position 134 is the single row setting, and is corresponding with the multichannel adapter. As shown in fig. 5, a vent hole 135 is provided in the gun discharging cavity 132, and when the piston 141 and the adapter 131 are inserted into the gun discharging cavity 132, an air outlet at the upper end of the adapter 131 and an air outlet at the lower end of the piston 141 realize the passage of an air passage through the vent hole 135, thereby realizing the communication of the air passage during the liquid suction and injection operation of the whole device. Because the crisscross setting of adapter and piston, make this multichannel integration application of sample device 100's structure more exquisite, space utilization is higher, does benefit to the miniaturization of equipment.

As shown in fig. 2, 6 and 8, the gun arraying module 130 further includes a needle releasing plate 136, a needle releasing rod 137 and a photoelectric switch detecting plate 150, the needle releasing plate 136 is disposed below the gun arraying cavity 132, photoelectric switch blocking sheet notches 151 are disposed on edges of front and rear sides of the needle releasing plate 136, a plurality of needle releasing photoelectric switches 152 are disposed on the photoelectric switch detecting plate 150, the photoelectric switch detecting plates 150 are disposed on front and rear sides of the needle releasing plate 136, the needle releasing photoelectric switches 152 correspond to the photoelectric switch blocking sheet notches 151, and two ends of the needle releasing rod 137 are respectively connected to the piston connecting plate 142 and the needle releasing plate 136. Further, the upper end of the needle-off rod 137 is provided with a spring connected with the piston connecting plate 142. When the needle removing operation is needed, the first motor 121 drives the piston connecting plate 142 to move downwards, the piston connecting plate 142 compresses the needle removing rod 137, the needle removing rod 137 pushes the needle removing plate 136 to move downwards, and then the needle removing plate 136 pushes the disposable sample adding needle on the adapter to move downwards, so that the needle removing action is completed. Photoelectric switch separation blade notches 151 are formed in the edges of the front side and the rear side of the needle releasing plate 136 and used for inserting needle taking photoelectric switch separation blades 156 on a needle releasing sleeve 153, photoelectric switch detection plates 150 are further arranged in front of and behind the needle releasing plate 136, a plurality of needle releasing photoelectric switches 152 are arranged on each photoelectric switch detection plate 150 side by side, namely, each channel is correspondingly provided with one needle releasing photoelectric switch 152, and needle releasing and needle taking in-place detection of each channel can be accurately achieved.

As shown in fig. 2, the rifle module 130 also includes a Z-direction photoelectric switch 179 for detecting the Z-direction movement position of the rifle module 130. The arrangement gun module 130 is provided with a Z-direction photoelectric switch blocking piece 178 corresponding to the Z-direction photoelectric switch 179, and after the arrangement gun module 130 moves in place, the Z-direction photoelectric switch blocking piece 178 enters the sensing range of the Z-direction photoelectric switch 179, so that Z-direction movement in-place detection of the arrangement gun module 130 is realized.

As shown in fig. 2 and fig. 7 to 9, the gun arranging module 130 further includes needle removing sleeves 153 with the same number as the channels, the needle removing sleeves 153 are sleeved on the adapter 131 and are alternately arranged in the front row and the rear row of the adapter 131, the needle removing sleeves 153 are fixed on the needle removing plate 136 through needle removing bolts 154, needle removing springs 155 are sleeved on the needle removing bolts 154, needle taking photoelectric switch blocking sheets 156 are arranged on the upper portions of the needle removing sleeves 153, and the needle taking photoelectric switch blocking sheets 156 can be inserted into photoelectric switch blocking sheet notches on the needle removing plate and are matched with photoelectric switches arranged on a photoelectric switch blocking plate for detecting needle taking and needle removing in place. As shown in fig. 10, take off needle cover 153 and set up in the front and back two rows of adapter 131 in a staggered manner, make in the limited length within range that sets up multichannel adapter side by side, make full use of degree of depth space, guarantee to get the needle, take off the needle operation smoothness and can accurately go on the detection that targets in place to make this row of rifle module 130's structure more exquisite, space utilization is higher, does benefit to the miniaturization of equipment. When the needle taking photoelectric switch blocking piece 156 on the needle removing sleeve 153 enters the detection range of the needle removing photoelectric switch 152, namely when the needle is taken in place by the disposable sample adding needle, the needle removing photoelectric switch 152 receives the sensing signal; when the needle taking photoelectric switch blocking piece 156 on the needle removing sleeve 153 leaves the detection range of the needle removing photoelectric switch 152, namely the needle removing plate 136 pushes the needle removing sleeve 153 to carry out needle removing operation in place, the needle removing photoelectric switch 152 has no sensing signal, so that the detection of needle taking in place and needle removing in place is realized.

As shown in fig. 2, 11 and 12, the rifle discharging module 130 further includes an air pressure detecting module 160, and the air pressure detecting module 160 is disposed on the left and right sides of the rifle discharging cavity 132, is communicated with the channel inside the piston, and is used for detecting the change of the liquid volume during liquid suction or liquid injection. This atmospheric pressure detection module 160 is through vent hole and the inside passageway intercommunication of piston in the row rifle cavity 132, atmospheric pressure change in the passageway when can real-time detection piston up-and-down motion to the change of the liquid capacity in the detection channel, and then detect imbibition or notes liquid action. The left side and the right side of the gun discharging cavity 132 are respectively provided with the detection modules 160, so that the condition that air pressure detection is inaccurate due to the blockage of the middle individual channel can be prevented, if the detection modules 160 on the left side and the right side of the gun discharging cavity 132 have the same change trend, the liquid suction or injection function is reflected to be normal, and if the change trends of the detection modules 160 on the left side and the right side of the gun discharging cavity 132 are inconsistent, the blockage of a certain channel or the abnormality of liquid suction and injection is reflected. Further, when the air pressure detection modules 160 on the left and right sides of the gun discharging cavity 132 receive the synchronous increase of the pressure detection signal, a liquid taking detection normal signal is sent out; when the air pressure detection modules 160 on the left side and the right side of the gun discharging cavity 132 receive the synchronous reduction of the pressure detection signals, normal liquid injection detection signals are sent out; when the air pressure detection modules 160 on the left and right sides of the gun discharging cavity 132 receive asynchronous pressure detection signals, an abnormal liquid taking or injecting signal is sent out.

As shown in fig. 2, 11 and 12, the air pressure detecting module 160 includes an air pressure detecting plate 161, a sealing ring 162, a spring 163 and a spring cover 164, the sealing ring 162 is disposed between the air pressure detecting plate 161 and the passage at the lower end of the piston, and the spring 163 and the spring cover 164 are sequentially disposed outside the air pressure detecting plate 161 to seal the passage inside the piston 141. This atmospheric pressure detects board 161 and realizes atmospheric pressure to detect the sealed of board 161 and inner channel through inboard sealing washer 162 and spring housing 164 to the tension of outside spring 163, guarantees the accuracy that atmospheric pressure detected, and simple structure is exquisite simultaneously, and space utilization is high, does benefit to the miniaturization of equipment.

The multi-channel integrated sample adding device 100 further comprises a control device, wherein the control device is electrically connected with components such as the Z-direction driving device 111, the first motor 121, the needle-off photoelectric switch 152, the air pressure detection plate 161, the Z-direction photoelectric switch 179 and the like respectively, and is used for sending a motion instruction of each module, receiving detection signals of the photoelectric switch and the air pressure detection module, comparing the detection signals and sending an abnormal information prompt.

The invention also provides a sample adding method of the multi-channel integrated sample adding device, which adopts the multi-channel integrated sample adding device and comprises the following steps:

when the sample adding mechanism 120 moves to the upper side of the needle taking position, the Z-direction driving device 111 drives the sample adding mechanism 120 to move downward, and the end of the adapter 131 is inserted into the opening of the sample adding needle to perform the needle taking operation. The application of sample mechanism 120 can move according to the position of placing of application of sample needle, has X, Y and Z to the degree of freedom, the flexibility ratio is high, the location is accurate to Z is to drive application of sample mechanism 120 downstream through Z to guide rail and Z to the slider cooperation to Z to drive application of sample mechanism 120, makes application of sample mechanism 120 have great power, overcomes adapter and application of sample needle open-ended frictional resistance when getting the needle, makes and gets the unusual emergence probability of needle lower, and it is comparatively stable to get the needle action.

When the sample adding mechanism 120 moves to the upper side of the liquid absorbing position and the Z-direction driving device 111 drives the sample adding needle on the adapter 131 to move downwards to the lower side of the liquid surface to be absorbed, the first motor 121 drives the piston 141 to move upwards, so that the adapter 131 communicated with the piston 141 performs liquid absorbing work.

The sample adding mechanism 120 moves to the sample adding position, and the first motor 121 drives the piston 141 to move downwards, so that the adapter communicated with the piston 141 performs liquid injection work. This first motor 121 is the step motor, through the corotation of first motor 121 or reversal, realizes inhaling notes liquid operation to inhale notes liquid process is more accurate, stable.

The sample adding mechanism 120 moves to the needle releasing area, the first motor 121 drives the piston connecting plate 142 to move downwards, the piston connecting plate 142 extrudes the needle releasing rod 137 to move downwards, the needle releasing rod 137 drives the needle releasing plate 136 to move downwards, and then the needle releasing sleeve 153 is driven to extrude the sample adding needle downwards to realize needle releasing. After the first motor 121 drives the piston 141 to move downwards for injection, the first motor 121 continues to drive the piston connecting plate 142 to move downwards, so that the needle release rod 137 is extruded to push the needle release plate 136 to move downwards, and the needle release action is further completed. This first motor 121 not only can drive and realize inhaling notes liquid operation, can drive moreover and realize taking off the needle operation, makes this multichannel integration application of sample device integrated level improve, simple structure is exquisite simultaneously, and space utilization is high, does benefit to the miniaturization of equipment.

Further, the sample adding method of the multi-channel integrated sample adding device is characterized by further comprising the following steps:

the sample adding needle pushes the needle releasing sleeve 153 to move upwards, and the needle taking photoelectric switch blocking piece 156 on the needle releasing sleeve 153 enters the detection range of the needle releasing photoelectric switch 152 to carry out needle taking in-place detection. Each channel adapter corresponds to one needle-taking photoelectric switch blocking piece 156 and the needle-off photoelectric switch 152, so that in-place detection of needle taking can be realized for each channel, and the detection accuracy is high. After the needle taking action, if a certain channel does not detect the induction signal of the needle-off photoelectric switch 152, the failure of needle taking of the channel is prompted, and the needle taking condition of each channel can be accurately detected.

The needle release plate 136 drives the needle release sleeve 153 to move downwards, and the needle taking photoelectric switch blocking piece 156 on the needle release sleeve 153 moves out of the detection range of the needle release photoelectric switch 152 to perform needle release in-place detection. The needle-removing in-place detection is realized by matching the needle-removing photoelectric switch blocking piece 156 with the needle-removing photoelectric switch 152, which is the same as the needle-removing in-place detection, and is beneficial to high integration and miniaturization of equipment. The in-place detection of the needle release can be realized through each channel, and the detection accuracy is high. After the needle-off action, if a certain channel still detects the sensing signal of the needle-off photoelectric switch 152, the channel is prompted to fail to take off the needle, and the needle-off condition of each channel can be accurately detected.

When the first motor 121 drives the piston 141 to move upward and the adaptor 131 connected to the piston 141 performs a liquid suction operation, the air pressure detection modules 160 on the left and right sides of the gun discharging chamber 132 perform air pressure detection. When the air pressure detection modules on the left side and the right side of the cavity of the discharging gun receive the synchronous increase of the pressure detection signals, a liquid taking detection normal signal is sent out; when the air pressure detection modules on the left side and the right side of the gun discharging cavity receive the synchronous reduction of the pressure detection signals, normal liquid injection detection signals are sent out; when the air pressure detection modules on the left side and the right side of the gun discharging cavity receive asynchronous pressure detection signals, liquid taking or injecting abnormal signals are sent out. The left side and the right side of the gun arranging cavity 132 are respectively provided with the detection modules 160, the condition that air pressure detection is inaccurate due to the blockage of each channel in the middle can be prevented, if the detection modules 160 on the left side and the right side of the gun arranging cavity 132 have the same change trend, the liquid suction or injection function is reflected to be normal, if the change trends of the detection modules 160 on the left side and the right side of the gun arranging cavity 132 are inconsistent, the blockage of a certain channel or the abnormal liquid suction and injection is reflected, and the real-time detection of the liquid suction and injection conditions of each channel is realized.

The multichannel integrated sample adding device provided by the invention realizes the operation of multichannel needle taking, liquid sucking, liquid injecting, needle releasing and the like through the integral matching of the driving device, the piston assembly and the gun arranging module, solves the problems of less parallel channels and low efficiency in the prior art, and solves the problems of larger size, low integration level and low flexibility of multichannel sample adding equipment in the prior art.

The applicant asserts that the above-described embodiments represent only the basic principles, main features and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, and that various changes and modifications can be made by one skilled in the art without departing from the spirit and scope of the invention, which will fall within the scope of the invention as claimed.

The present invention is not limited to the above embodiments, and all embodiments using the similar structure and method to achieve the object of the present invention are within the protection scope of the present invention.

Claims (10)

1. A multi-channel integrated sample adding device is characterized by comprising a base, wherein a Z-direction driving device, a Z-direction guide rail, a Z-direction sliding block and a sample adding mechanism are arranged on the base;

the Z-direction driving device can drive a Z-direction sliding block on the Z-direction guide rail to enable the Z-direction sliding block to move up and down on the Z-direction guide rail, the sample adding mechanism is arranged on the Z-direction sliding block on the Z-direction guide rail, and the sample adding mechanism can be driven by the Z-direction driving device to move up and down along the Z-direction guide rail;

the application of sample mechanism includes first motor, piston assembly, row rifle module, piston assembly includes the piston the same with passageway quantity, arrange the rifle module and include the adapter the same with passageway quantity, piston assembly connect in the output of first motor, arrange the rifle module set up in piston assembly's lower extreme, the piston with the adapter link up continuously.

2. The multi-channel integrated sample adding device according to claim 1, wherein the number of the channels is eight.

3. The multi-channel integrated sample adding device according to claim 1, wherein the piston assembly further comprises a piston connecting plate, the pistons are arranged in front and back rows, the first row of pistons and the second row of pistons are arranged in a staggered and spaced manner, and the upper ends of the pistons are connected with the piston connecting plate.

4. The multi-channel integrated sample adding device according to claim 3, wherein the gun arranging module further comprises a gun arranging cavity, a piston mounting position is arranged on the upper portion of the gun arranging cavity, an adapter mounting position is arranged on the lower portion of the gun arranging cavity, the piston mounting positions are arranged in a front row and a rear row in a staggered manner, the adapter mounting positions are arranged in the middle of the two rows of piston mounting positions, the lower ends of the pistons are inserted into the piston mounting positions, the upper ends of the adapters are inserted into the adapter mounting positions, vent holes are formed in the gun arranging cavity, and the upper ends of the adapters and the lower ends of the pistons realize the passage of the gas circuit through the vent holes.

5. The multi-channel integrated sample adding device according to claim 4, wherein: the automatic needle releasing device is characterized in that the gun arranging module further comprises a needle releasing plate, a needle releasing rod and a photoelectric switch detection plate, the needle releasing plate is arranged below the gun arranging cavity, photoelectric switch separation blade notches are formed in the edges of the front side edge and the rear side edge of the needle releasing plate, a plurality of needle releasing photoelectric switches are arranged on the photoelectric switch detection plate, the photoelectric switch detection plate is arranged on the front side edge and the rear side edge of the needle releasing plate, the photoelectric switches correspond to the photoelectric switch separation blade notches, and the two ends of the needle releasing rod are respectively connected with the piston connecting plate and the needle releasing plate.

6. The multi-channel integrated sample adding device according to claim 5, wherein the gun arranging module further comprises needle removing sleeves with the same number as the channels, the needle removing sleeves are sleeved on the adapter and are arranged in the front row and the rear row of the adapter in a staggered manner, the needle removing sleeves are fixed on the needle removing plate through anti-falling bolts, needle removing springs are sleeved on the anti-falling bolts, needle taking photoelectric switch blocking pieces are arranged on the upper portions of the needle removing sleeves, and the needle taking photoelectric switch blocking pieces can be inserted into photoelectric switch blocking piece notches in the needle removing plate and are matched with photoelectric switches arranged on the photoelectric switch blocking pieces for detecting the positions of needle taking and needle removing.

7. The multi-channel integrated sample adding device according to claim 1, wherein the gun discharging module further comprises air pressure detection modules, the air pressure detection modules are arranged on the left side and the right side of the gun discharging cavity and communicated with the channel inside the piston, and the air pressure detection modules are used for detecting the change of liquid capacity during liquid suction or liquid injection.

8. The multi-channel integrated sample adding device according to claim 7, wherein the air pressure detection module comprises an air pressure detection plate, a sealing ring, a spring and a spring cover, the sealing ring is arranged between the air pressure detection plate and the channel at the lower end of the piston, and the spring cover are sequentially arranged on the outer side of the air pressure detection plate and are used for sealing the air pressure detection plate and the channel inside the piston.

9. A sample adding method of a multi-channel integrated sample adding device, which is used for the multi-channel integrated sample adding device of claims 1-8, and comprises the following steps:

when the sample adding mechanism moves to the position above the needle taking position, the Z-direction driving device drives the sample adding mechanism to move downwards, and the tail end of the adapter is inserted into an opening of a sample adding needle to perform needle taking action;

when the sample adding mechanism moves to the position above the liquid absorbing position, and the Z-direction driving device drives the sample adding needle on the adapter to move downwards to the position below the liquid surface to be absorbed, the first motor drives the piston to move upwards, so that the adapter communicated with the piston performs liquid absorbing work;

the sample adding mechanism moves to a sample adding position, and the first motor drives the piston to move downwards so that the adapter communicated with the piston performs liquid injection work;

the sample adding mechanism moves to a needle releasing area, the first motor drives the piston connecting plate to move downwards, the piston connecting plate extrudes the needle releasing rod to move downwards, the needle releasing rod drives the needle releasing plate to move downwards, and then the needle releasing sleeve is driven to extrude the sample adding needle downwards to realize needle releasing.

10. The method for loading the sample by the multi-channel integrated sample loading device according to claim 9, further comprising the following steps:

the sample adding needle pushes the needle removing sleeve to move upwards, and a needle taking photoelectric switch blocking piece on the needle removing sleeve enters a needle removing photoelectric switch detection range to perform needle taking in-place detection;

the needle release plate drives the needle release sleeve to move downwards, and a needle taking photoelectric switch blocking piece on the needle release sleeve leaves out of the detection range of the needle release photoelectric switch to detect needle release in place;

the first motor drives the piston to move upwards, so that when the adapter communicated with the piston performs liquid suction work, the air pressure detection modules on the left side and the right side of the gun arranging cavity perform air pressure detection;

when the air pressure detection modules on the left side and the right side of the gun discharging cavity receive the synchronous increase of the pressure detection signal, a liquid taking detection normal signal is sent out;

when the air pressure detection modules on the left side and the right side of the gun discharging cavity receive the synchronous reduction of the pressure detection signal, a normal liquid injection detection signal is sent out;

when the air pressure detection modules on the left side and the right side of the gun discharging cavity receive asynchronous pressure detection signals, liquid taking or liquid injection abnormal signals are sent out.

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