CN114621829B - Antibacterial laundry detergent containing lysozyme - Google Patents

Abstract

The application relates to a laundry detergent with antibacterial effect, which comprises the following components in percentage by weight: 5-15% of lysozyme, 1-10% of anionic surfactant, 1-10% of nonionic surfactant, 1-5% of perfume, 0.1-0.5% of ethylenediamine tetraacetic acid and the balance of deionized water. The liquid laundry detergent disclosed by the application combines lysozyme with a specific anionic surfactant and a nonionic surfactant, has a synergistic effect on bacteriostasis of clothes, and has the advantages that antibacterial components in the liquid laundry detergent select the lysozyme with natural sources, the safety is good, the preparation method is simple, and the liquid laundry detergent is suitable for industrial production.

Description

Antibacterial laundry detergent containing lysozyme

Technical Field

The application relates to the field of daily chemicals, in particular to a liquid laundry detergent containing lysozyme.

Background

With the recent outbreak of influenza and new coronaviruses, consumers are increasingly paying attention to washing products having antibacterial and antiseptic functions. The antibacterial detergent can effectively remove bacterial and virus remained on clothes, reduce the harm of the bacterial and virus to human health, and can protect the body and skin of a user and prevent the infection and regeneration of the bacterial and virus while having the functions of wetting, emulsifying, dispersing, solubilizing and cleaning. With the improvement of the quality of life and the material level of people, the detergent with the antibacterial and bacteriostatic functions becomes an indispensable product in life.

The bacteriostat is a substance which can kill bacteria and inhibit the growth of bacteria within a certain period of time and mainly comprises halogen systems, oxygen systems, aldehydes, phenols, quaternary ammonium salts, guanidine, cations, natural plant extracts and the like, but various problems can be caused when the bacteriostat is compounded with a laundry detergent, so that the use range of the bacteriostat becomes limited, and the selection of the bacteriostat is important.

At present, triclosan and triclocarban Ban Yijun are relatively hot, have the effects of effectively killing and inhibiting bacteria, and are commonly used in daily chemical washing industry in recent years, but are gradually eliminated in the washing industry due to various safety problems such as mammal reproductive dysfunction, cancerogenic risk, dysplasia and the like. Phenolic bactericides achieve the aim of sterilization by destroying the cell membrane of the thallus to denature the protein, but the use in the formulation is affected due to the problems of light and thermochromic. In recent years, the antibacterial components extracted from plants are from nature, are safe and nontoxic, are favored by consumers, but have weaker antibacterial performance and relatively higher price, so that the consumers can expect to stop. Therefore, although the bacteriostat is various, the compatibility with other components in the formula and the safety of the bacteriostat limit the use, and the selection of the proper bacteriostat is the core.

Biological enzyme preparations are often used as an adjunct in laundry detergents, as a widely used component in powder and household liquid detergents, soil release agents, automatic dishwashing detergents and industrial cleaning products. The protease can remove blood stains difficult to wash, the lipase can effectively clean severe greasy dirt, and the cellulase has a relatively hot research in recent years, so that the effect of brightening and softening the clothing can be achieved. The biological enzyme can be biodegraded without leaving harmful residues, and can reduce the negative influence of chemical substances such as surfactants and the like on the environment.

Lysozyme is a basic protein which is soluble in water and can achieve the aim of sterilization by breaking the beta-1, 4-glycosidic bond in peptidoglycan, the basic component of the bacterial cell wall. The action mechanism of lysozyme is mainly represented by its biochemical function, and the cell wall of the microorganism is destroyed by catalyzing the hydrolysis of peptidoglycan layer which is the basic component of the cell wall so as to achieve the purpose of lysozyme, the cell wall of the microorganism plays an important role in physiological and biochemical functions, such as immunity, pathogenicity and survival of the microorganism, and the action site of lysozyme is the peptidoglycan layer of the cell wall of the microorganism, so that the difference of the cell wall structure of the microorganism has great influence on the action effect of lysozyme. Lysozyme has been widely used in the fields of medicine, food, animal husbandry, etc., but there are few reports on its use in the field of daily chemicals.

At present, no report related to antibacterial liquid laundry detergent which takes lysozyme and surfactant as main antibacterial components is found.

Disclosure of Invention

The first aspect of the application provides a laundry detergent with antibacterial effect, which comprises the following components in percentage by weight: 5-15% of lysozyme, 1-10% of anionic surfactant, 1-10% of nonionic surfactant, 1-5% of perfume, 0.1-0.5% of ethylenediamine tetraacetic acid and the balance of deionized water.

Preferably, the anionic surfactant is selected from one or more of fatty acid Methyl Ester Sulfonate (MES), sodium dodecyl benzene sulfonate (LAS) and sodium lauroyl sarcosinate (S-12).

More preferably, the anionic surfactant is sodium lauroyl sarcosinate (S-12).

Preferably, the nonionic surfactant is selected from one or more of modified oil ethoxylate (SOE-N-60), fatty acid methyl ester ethoxylate (FMEE), and alkyl glycoside APG1214.

More preferably, the nonionic surfactant is the alkyl glycoside APG1214.

Preferably, the weight ratio of lysozyme, anionic surfactant and nonionic surfactant in the liquid laundry detergent is 4 (1-3): 2-5.

More preferably, the weight ratio of lysozyme, anionic surfactant and nonionic surfactant in the laundry detergent is 4:1:3.

Preferably, the liquid laundry detergent is composed of the following components in percentage by weight: 10% of lysozyme, 2-8% of anionic surfactant, 2-8% of nonionic surfactant, 1% of perfume, 0.1% of ethylenediamine tetraacetic acid and the balance of deionized water.

More preferably, the laundry detergent consists of the following components in percentage by weight: 10% of lysozyme, 2.5% of anionic surfactant, 7.5% of nonionic surfactant, 1% of perfume, 0.1% of ethylenediamine tetraacetic acid and the balance of deionized water.

Further preferably, the laundry detergent consists of the following components in percentage by weight: 10% of lysozyme, 2.5% of sodium lauroyl sarcosinate (S-12), 12147.5% of alkyl glycoside APG, 1% of perfume, 0.1% of ethylenediamine tetraacetic acid and the balance of deionized water.

A second aspect of the present application provides a method for preparing the above laundry detergent, comprising the steps of:

(1) Weighing the prescription amount of anionic surfactant and nonionic surfactant, adding a proper amount of deionized water, and fully stirring for dissolution;

(2) Sequentially adding lysozyme, perfume and ethylenediamine tetraacetic acid with the prescription amount, and fully stirring for dissolution;

(3) And continuously adding the balance of deionized water to the prescription amount of the laundry detergent to obtain the laundry detergent.

A third aspect of the present application provides the use of a laundry detergent as described above for the bacteriostasis of laundry.

Preferably, the antibacterial is one or more of inhibiting escherichia coli and candida albicans.

More preferably, the antibacterial is simultaneous inhibition of E.coli and Candida albicans.

Further preferably, the bacteriostatic is simultaneous inhibition of escherichia coli ATCC 8099 and candida albicans ATCC 10231.

The application has the positive beneficial effects that:

surprisingly, through repeated experiments, the application surprisingly found that the combination of lysozyme with specific anionic surfactant and nonionic surfactant has a synergistic effect on bacteriostasis of clothes. In addition, the antibacterial component of the liquid laundry detergent provided by the application is the lysozyme with natural sources, so that the liquid laundry detergent is good in safety, simple in preparation method and suitable for industrial production.

Detailed Description

The present application will be further described with reference to examples, but the embodiments of the present application are not limited thereto. The experimental methods used in the following examples are conventional methods unless otherwise specified. The lysozyme used in examples and test examples was lysozyme extracted from egg white.

Example 1 (lysozyme: sodium lauroyl sarcosinate S-12: alkyl glycoside APG1214 = 4:1:3)

2.5g of sodium lauroyl sarcosinate S-12 and 7.5g of alkyl glycoside APG1214 are respectively weighed, 70g of deionized water is added after mixing, and 10g of lysozyme, 1g of perfume and 0.1g of ethylenediamine tetraacetic acid are sequentially added for dissolution, and the balance of deionized water is added after dissolution, so that the laundry detergent with the weight of 100g of the final solution is prepared.

Example 2 (lysozyme: sodium lauroyl sarcosinate S-12: alkyl glycoside APG1214 = 4:2:2)

5g of sodium lauroyl sarcosinate S-12 and 5g of alkyl glycoside APG1214 are respectively weighed, 70g of deionized water is added after mixing, the mixture is fully stirred and dissolved, 10g of lysozyme, 1g of perfume and 0.1g of ethylenediamine tetraacetic acid are sequentially added, and the balance of deionized water is added after dissolving, so that the laundry detergent with the weight of 100g of final solution is prepared.

Example 3 (lysozyme: sodium moon Gui Ji base amino acid S-12: alkyl glycoside APG 1214=10:1:9)

1g of Gui Ji-base sodium amino acid S-12 and 9g of alkyl glycoside APG1214 are respectively weighed, 70g of deionized water is added after mixing, the mixture is fully stirred and dissolved, 10g of lysozyme, 1g of perfume and 0.1g of ethylenediamine tetraacetic acid are sequentially added, and the balance of deionized water is added after dissolving, so that the final solution weight is 100g of laundry detergent.

Example 4 (lysozyme: fatty acid methyl ester sulfonate: alkyl glycoside apg1214=4:1:3)

2.5g of fatty acid methyl ester sulfonate MES and 7.5g of alkyl glycoside APG1214 are respectively weighed, 70g of deionized water is added after mixing, and 10g of lysozyme, 1g of perfume and 0.1g of ethylenediamine tetraacetic acid are sequentially added for dissolving, and the balance of deionized water is added after dissolving, so that the laundry detergent with the weight of 100g of the final solution is prepared.

Example 5 (lysozyme: sodium lauroyl sarcosinate S-12: fatty acid methyl ester ethoxylate=4:1:3)

2.5g of lauroyl sarcosinate S-12 and 7.5g of fatty acid methyl ester ethoxylate FMEE are respectively weighed, 70g of deionized water is added after mixing, and 10g of lysozyme, 1g of perfume and 0.1g of ethylenediamine tetraacetic acid are sequentially added after full stirring and dissolution, and the balance of deionized water is added after dissolution, so that the laundry detergent with the weight of 100g of the final solution is prepared.

Comparative example 1 (lysozyme: sodium lauroyl sarcosinate S-12=1:1)

10g of lauroyl sarcosine sodium S-12 is weighed, 70g of deionized water is added after mixing, and then the mixture is fully stirred and dissolved, 10g of lysozyme, 1g of spice and 0.1g of ethylenediamine tetraacetic acid are sequentially added, and the balance of deionized water is added after dissolving, so that the laundry detergent with the weight of 100g of final solution is prepared.

Comparative example 2 (lysozyme: alkyl glycoside apg1214=1:1)

Weighing 10g of alkyl glycoside APG1214, mixing, adding 70g of deionized water, stirring thoroughly for dissolution, sequentially adding 10g of lysozyme, 1g of perfume and 0.1g of ethylenediamine tetraacetic acid, dissolving, and adding the balance of deionized water to prepare the laundry detergent with the final solution weight of 100 g.

Test example 1, antibacterial effect of the laundry detergent of the present application

1. Test method

Fresh test bacteria (E.coli ATCC 8099 and Candida albicans ATCC 10231) cultured for 24 hours are washed by PBS with pH7.4, 20 mu L of the mixture is dripped on a sterile cloth piece (1 cm multiplied by 1 cm) after the mixture is uniformly mixed, the sterile cloth piece is prepared into a bacterial piece, the bacterial piece is dried for 30 minutes at 37 ℃, and the number of recovered bacterial pieces is (1-9) multiplied by 104 cfu/piece.

Referring to the liquid laundry formulations of examples 1 to 5 and comparative examples 1 to 2 described above, the perfume and ethylenediamine tetraacetic acid added thereto were omitted, and the samples of examples 1 to 5 and comparative examples 1 to 2 used in test example 1 were prepared, wherein the total amount of lysozyme and surfactant in the samples of each test group was 20% by weight.

Taking the bacterial slices, respectively adding 5mL of sample solutions of the groups 1-5 and the groups 1-2 of the examples, shaking uniformly to enable the bacterial slices to fully act, timing, respectively taking out the bacterial slices after acting for 10min, adding the bacterial slices into 5mL of PBS, uniformly mixing, counting, taking 2-3 dilutions, respectively sucking 1mL of pouring nutrient agar medium (a Candida albicans culture medium), culturing for 48h in a culture box at 37 ℃, and then carrying out viable count. The test was repeated three times by setting a negative control group and a positive control group at the same time. The test result antibacterial rate is calculated according to the following formula:

bacteriostasis% = (average colony count of control group-average colony count of test group)/average colony count of control group x 100%.

2. Test results

The antibacterial effect of the antibacterial components of the laundry detergent of each test group of the present application after 10 minutes of interaction with E.coli and Candida albicans is shown in Table 1 below.

TABLE 1 antibacterial results of the laundry detergent of the present application on E.coli and Candida discolour

Test group	Coliform bacteria inhibition rate (%)	Candida albicans antibacterial ratio (%)
			Example 1 group	92.47％	87.25％
Example 2 group	79.43％	75.41％
			Example 3 group	74.28％	76.52％
Example 4 group	62.49％	60.63％
			Example 5 group	58.43％	56.34％
Comparative example 1 group	43.28％	47.25％
			Comparative example 2 group	48.49％	42.52％

As can be seen from table 1 above, compared with comparative examples 1-2, the antibacterial rates of the laundry detergent of examples 1-5 of the present application on escherichia coli and candida albicans are both more than 50%, and it is confirmed that the antibacterial effect of the lysozyme of the present application combined with specific anionic surfactant and nonionic surfactant is significantly better than that of lysozyme alone combined with anionic surfactant or nonionic surfactant. Wherein the bacteriostatic effect of example 1 group was optimal, resulting in an unexpected excellent technical effect.

Claims (7)

1. The laundry detergent with the antibacterial effect is characterized by comprising the following components in percentage by weight: 5-15% of lysozyme, 1-10% of anionic surfactant, 1-10% of nonionic surfactant, 1-5% of perfume, 0.1-0.5% of ethylenediamine tetraacetic acid and the balance of deionized water; the anionic surfactant is sodium lauroyl sarcosinate,

the nonionic surfactant is alkyl glycoside APG1214; the weight ratio of lysozyme to anionic surfactant to nonionic surfactant in the liquid laundry detergent is 4 (1-3) to 2-5.

2. The liquid laundry detergent of claim 1, wherein the weight ratio of lysozyme, anionic surfactant and nonionic surfactant in the liquid laundry detergent is 4:1:3.

3. The liquid laundry detergent of claim 1, wherein the liquid laundry detergent consists of the following components in weight percent: 10% of lysozyme, 2-8% of anionic surfactant, 2-8% of nonionic surfactant, 1% of perfume, 0.1% of ethylenediamine tetraacetic acid and the balance of deionized water.

4. A laundry detergent according to claim 3, characterized in that it consists of the following components in weight percent: 10% of lysozyme, 2.5% of anionic surfactant, 7.5% of nonionic surfactant, 1% of perfume, 0.1% of ethylenediamine tetraacetic acid and the balance of deionized water.

5. The liquid laundry detergent of claim 4, wherein the liquid laundry detergent consists of the following components in weight percent: 10% of lysozyme, 2.5% of sodium lauroyl sarcosinate, 7.5% of alkyl glycoside APG1214, 1% of perfume, 0.1% of ethylenediamine tetraacetic acid and the balance of deionized water.

6. A process for preparing a laundry detergent as claimed in any one of claims 1 to 5, comprising the steps of:

(1) Weighing the prescription amount of anionic surfactant and nonionic surfactant, adding a proper amount of deionized water, and fully stirring for dissolution;

(2) Sequentially adding lysozyme, perfume and ethylenediamine tetraacetic acid with the prescription amount, and fully stirring for dissolution;

(3) And continuously adding the balance of deionized water to the prescription amount of the laundry detergent to obtain the laundry detergent.

7. Use of a laundry detergent according to any one of claims 1 to 5 for laundry bacteriostasis.