CN114525202A - Closed detection card for detecting new coronavirus - Google Patents
Closed detection card for detecting new coronavirus Download PDFInfo
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- CN114525202A CN114525202A CN202210150777.0A CN202210150777A CN114525202A CN 114525202 A CN114525202 A CN 114525202A CN 202210150777 A CN202210150777 A CN 202210150777A CN 114525202 A CN114525202 A CN 114525202A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
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Abstract
The invention discloses a closed detection card for detecting new coronavirus, which comprises: the device comprises a detection assembly, a sampling assembly adjacent to the detection assembly, and a sealing assembly arranged between the detection assembly and the sampling assembly; the detection assembly comprises a detection shell and a vacuum tube longitudinally arranged in the detection shell, wherein detection test paper is arranged in the vacuum tube, and a sealed transparent panel corresponding to the vacuum tube is arranged on the detection shell; the sealing assembly comprises a connecting pipe which penetrates through the detection shell and is communicated with the vacuum pipe, and a movable pipe which is connected with the sampling pipe of the sampling assembly and is movably connected with the connecting pipe, wherein a sealing plug is arranged in the connecting pipe, a plug puncturing needle head which is communicated with the sampling pipe is arranged in the movable pipe, and the movable pipe is driven to enable the plug puncturing needle head to puncture the sealing plug and enable the connecting pipe to be communicated with the movable pipe. The sampling assembly and the detection assembly accelerate the detection speed and simplify the detection steps; by enclosing the assembly, cross-contamination and contact contamination are reduced.
Description
Technical Field
The invention relates to the technical field of virus detection, in particular to a closed detection card for detecting new coronavirus.
Background
The description of the background of the invention pertaining to the related art to which this invention pertains is given for the purpose of illustration and understanding only of the summary of the invention and is not to be construed as an admission that the applicant is explicitly or implicitly admitted to be prior art to the date of filing this application as first filed with this invention.
Because the virus load in the cold chain fresh food or the related environment is low, the conventional sampling and detecting method has the risk of missing detection, and the conventional nucleic acid detecting method (such as a PCR method) has the problems of high requirement on the environment, long detecting time and high operation difficulty, the conventional detecting device cannot be completely closed, and the problems of easy cross infection, safety of contagion to contactants and the like easily occur.
Disclosure of Invention
The invention aims to provide a closed detection card for detecting new coronavirus, which aims to solve the problem that the existing new coronavirus detection card cannot be completely closed, so that cross infection and infection occur.
The technical scheme for solving the technical problems is as follows:
a closed detection card for detecting a new coronavirus, comprising: the device comprises a detection assembly, a sampling assembly adjacent to the detection assembly, and a sealing assembly arranged between the detection assembly and the sampling assembly;
the detection assembly comprises a detection shell and a vacuum tube longitudinally arranged in the detection shell, wherein detection test paper is arranged in the vacuum tube, and a sealed transparent panel corresponding to the vacuum tube is arranged on the detection shell;
the sealing assembly comprises a connecting pipe which penetrates through the detection shell and is communicated with the vacuum pipe, and a movable pipe which is connected with the sampling pipe of the sampling assembly and is movably connected with the connecting pipe, wherein a sealing plug is arranged in the connecting pipe, a puncture plug needle head communicated with the sampling pipe is arranged in the movable pipe, and the movable pipe is driven to enable the puncture plug needle head to puncture the sealing plug and enable the connecting pipe to be communicated with the movable pipe.
The beneficial effects of adopting the above technical scheme are: by the closing assembly, when the detection card is not used, the sampling assembly and the detection assembly are in an unconnected state through the sealing plug in the connecting pipe. When the detection card is used for detection, the sampling assembly samples and reacts, then the movable pipe is driven to rotate around the connecting pipe or the movable pipe is pressed, and the movable tube and the connecting tube generate axial displacement, the puncture needle head generates axial displacement along with the axial displacement, thereby piercing through the sealing plug and extending into the connecting pipe, the connecting pipe is communicated with the movable pipe, sucking the reaction solution in the sampling assembly into the vacuum tube under the action of the vacuum tube and contacting with the detection test paper, if the sample sampled in the sampling assembly has new coronavirus, reacting and developing the color of the detection test paper, observing whether the detection test paper develops the color by a detector according to the sealed transparent panel, therefore, whether the new coronavirus is carried or not is judged, the detection speed is increased, the detection steps are simplified, the detection assembly is closed, the contact probability of detection personnel is reduced, and the infection probability is reduced.
Further, the sampling subassembly is still including dismantling the sealed lid that sets up at the sampling tube end portion and set up at the inboard sampling swab of sealed lid, is provided with the reaction layer in the sampling tube, and the sampling swab sets up and stretches into to the reaction layer along the sampling tube axial.
The beneficial effects of adopting the above technical scheme are: the sampling swab is through wiping the sample that awaits measuring and sampling, then puts into the sampling pipe with the sampling swab after the sampling, and the reaction layer in the sampling pipe carries out reaction treatment to the sample on the sampling swab to obtain nucleic acid solution, simplified the detection step through the sampling subassembly, accelerated follow-up detection speed, easy operation is convenient.
Further, the sealing cover is in threaded connection with the sampling pipe.
The beneficial effects of adopting the above technical scheme are: through threaded connection, the sealing cover is convenient to detach and mount.
Furthermore, the inner side of the sealing cover is provided with an elastic sealing plug matched with the inner diameter of the sampling pipe, and the sampling swab penetrates through the elastic sealing plug and is connected with the sealing cover.
The beneficial effects of adopting the above technical scheme are: the elastic sealing plug is used for ensuring the leakage of a cracking layer in the sampling tube and ensuring the sealing property of the sampling tube.
Further, the outer wall of connecting pipe is provided with the external screw thread, and the inner wall of activity pipe is provided with the internal thread with the external screw thread complex of the outer wall of connecting pipe.
The beneficial effects of adopting the above technical scheme are: the movable tube passes through threaded connection with the connecting pipe and realizes rotating, moreover, the steam generator is simple in structure, and be difficult for droing when rotating, rotation through the movable tube, drive the whole rotation of sampling subassembly, and make the puncture needle puncture the sealing plug and stretch into to the connecting pipe through rotating, the reaction layer after the reaction in the sampling subassembly this moment, under the effect of determine module's vacuum tube, the reaction layer is inhaled further and is contacted with test paper in the vacuum tube, can audio-visual observation test paper's the chromogenic condition in the vacuum tube through the sealed transparent panel on the determine housing, the testing personnel run through test paper according to the visual zone of casing and whether develop color, thereby judge whether to carry new hat virus.
Further, the inner wall of the end part of the movable pipe far away from the sampling pipe is provided with a sealing ring.
The beneficial effects of adopting the above technical scheme are: the sealing ring is used for ensuring the sealing performance between the movable pipe and the connecting pipe.
Further, a safety ring is arranged between the outer wall of the detection shell and the outer wall of the movable tube far away from the sampling tube.
The beneficial effects of adopting the above technical scheme are: the safety ring is used for fixing the movable pipe and the outer wall of the detection shell when the detection card is not used, so that the movable pipe and the connecting pipe cannot rotate and move; when needs use the detection card to examine time measuring, use the sampling swab to sample earlier, and stretch into the swab after will sampling and react in the reaction layer, then dismantle down the safety ring and order about the movable tube rotatory around the connecting pipe or press the movable tube and make it produce axial displacement with the connecting pipe, the interior stinging syringe needle of movable tube removes and punctures the sealing plug and stretch into to the connecting pipe thereupon, under the effect of detection components's vacuum tube, the reaction layer is inhaled further and is contacted with the detection test paper in to the vacuum tube.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, the sampling assembly and the detection assembly are adopted, so that the detection speed is increased, and the detection steps are simplified; through the closed assembly, the exposure probability of the sampling sample is reduced, and meanwhile, the contact probability of detection personnel is also reduced, so that the infection probability is reduced.
2. The detection card can be suitable for detecting new coronavirus, influenza A and influenza B, and the like, only the reaction layer and the detection test paper corresponding to the detection card are needed to be replaced, the operation is simple and convenient, the whole detection process is in a closed state, and the situations of cross infection, contact infection and the like cannot be generated.
Drawings
FIG. 1 is a schematic diagram of a specific structure of a test card;
FIG. 2 is a schematic view of the rotated structure of the detection card;
FIG. 3 is a schematic sectional view showing the detailed structure of the closure assembly and the sampling tube;
fig. 4 is a schematic view of the sealing plug being pierced by the piercing pin head.
In the figure: 1-detection component, 11-detection shell, 12-vacuum tube, 13-detection test paper, 14-sealed transparent panel, 2-sampling component, 21-sampling tube, 22-sealing cover, 221-elastic sealing plug, 23-sampling swab, 24-reaction layer, 3-sealing component, 31-connecting tube, 32-movable tube, 33-sealing plug, 34-puncture needle head and 4-safety ring.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it is to be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.
Examples
As shown in fig. 1 and 2, an enclosed test card for testing new coronavirus, comprising: the device comprises a detection assembly 1, a sampling assembly 2 adjacent to the detection assembly 1, and a sealing assembly 3 arranged between the detection assembly 1 and the sampling assembly 2; sampling subassembly 2 is used for gathering the sample, and detection component 1 is used for detecting whether the sample of gathering carries new coronavirus on the sampling subassembly 2, and seal assembly 3 is used for guaranteeing the leakproofness between detection component 1 and the sampling subassembly 2.
The detection assembly 1 comprises a detection shell 11 and a vacuum tube 12 longitudinally arranged in the detection shell 11, wherein the vacuum tube 12 is a transparent vacuum tube and is made of a PET material, and the vacuum tube 12 is vacuumized during production; the vacuum tube 12 is internally provided with a detection test paper 13, the detection shell 11 is provided with a sealed transparent panel 14 corresponding to the vacuum tube 12, the sealed transparent panel 14 is made of transparent PVC material, and the color development condition of the detection test paper 13 in the vacuum tube 12 can be visually observed through the sealed transparent panel 14; the test paper 13 is an existing immunochromatographic test paper.
As shown in fig. 1, the sampling assembly 2 includes a sampling tube 21 adjacent to the detection housing 11, a sealing cover 22 detachably disposed at an end of the sampling tube 21, and a sampling swab 23 disposed inside the sealing cover 22, wherein a reaction layer 24 is disposed in the sampling tube 21, and the sampling swab 23 is axially disposed along the sampling tube 21 and extends into the reaction layer 24. The sample to be tested includes but is not limited to the surface of an article, the oral cavity of a human body, and the sampling swab 23 is placed on the surface of the sample to be tested and is used for sampling by wiping. The reaction layer 24 adopts the existing RIPA lysate for extracting RNA of the new coronavirus, the lysate in the sampling tube 21 is 0.5ml to 1ml, the time of the lysis treatment is 2-10min, and the lysate is used for completely mixing and reacting the sample collected by the sampling swab 23 in the sampling tube 21 and the lysate.
The inner side of the sealing cover 22 is provided with an elastic sealing plug 221 matching with the inner diameter of the sampling tube 21, and the sampling swab 23 penetrates through the elastic sealing plug 221 and is connected with the sealing cover 22. The elastic sealing plug 221 is used to ensure the sealing performance of the sampling tube 21.
Sealed lid 22 realizes dismantling through threaded connection with sampling pipe 21 in this embodiment and is connected, and the inner wall of sealed lid 22 sets up the internal thread, and the external wall setting of the corresponding position department of sampling pipe 21 has realized threaded connection with the internal thread complex external screw thread of the inner wall of sealed lid 22. In other embodiments of the present invention, a clamping groove may be disposed on an inner wall of the sealing cover 22, and a clamping block is disposed on an outer wall of the sampling tube 21, so that the sealing cover 22 is clamped with the sampling tube 21, thereby achieving detachable connection.
As shown in fig. 3 and 4, the sealing assembly 3 includes a connecting pipe 31 penetrating through the detection housing 11 and communicating with the vacuum pipe 12, and a movable pipe 32 connected with the sampling pipe 21 of the sampling assembly 2 and movably connected with the connecting pipe 31, a sealing plug 33 is disposed in the connecting pipe 31, the connecting pipe 31 communicates with the vacuum pipe 12, the connecting pipe 31 and the vacuum pipe 12 are both vacuumized, and are in a sealing state through the sealing plug 33, and a certain negative pressure is ensured in the connecting pipe 31 and the vacuum pipe 12. The movable tube 32 is internally provided with a puncture needle 34 communicated with the sampling tube 21, the outer diameter of the puncture needle 34 is matched with the inner diameter of the connecting tube 31, after the sampling assembly 2 is subjected to sampling treatment and a cracking reaction, the movable tube 32 is rotated or pressed to enable the puncture needle 34 to penetrate through the sealing plug 33 and extend into the connecting tube 31, at the moment, the movable tube 32 is communicated with the connecting tube 31, the reacted lysate in the sampling tube 21 in the sampling assembly 2 is sucked into the vacuum tube 12 under the action of the vacuum tube 12 and is contacted with the detection test paper 13, if a sampled sample has new crown viruses, the detection test paper 13 is reacted and develops color, a detector observes whether the detection test paper 13 develops color according to the sealed transparent panel 14, and then judges whether the new crown viruses are carried or not, the detection speed is increased, and the detection steps are simplified.
In this embodiment, the connecting tube 31 is movably connected to the movable tube 32, and the movable connection mode specifically includes: a rotation mode and a pressing mode;
wherein the rotation mode mainly comprises: the outer wall of the connecting pipe 31 is provided with an external thread, the inner wall of the movable pipe 32 is provided with an internal thread matched with the external thread of the outer wall of the connecting pipe 31, the movable pipe 32 is connected through threads to rotate, and axial displacement can be generated when the movable pipe 32 rotates around the connecting pipe 31, so that the puncture plug needle 34 in the movable pipe 32 punctures the sealing plug 33, the connecting pipe 31 is communicated with the movable pipe 32, the lysate after the reaction of the sampling pipe 21 is sucked and reacts with the detection test paper 13 under the action of the vacuum pipe 12, and the operation is simple and convenient.
The pressing mode is as follows: the inner diameter of the movable tube 32 is matched with the outer diameter of the connecting tube 31, the movable tube 32 is pressed to axially displace towards the connecting tube 31, at the moment, the puncture needle 34 in the movable tube 32 moves along with the movable tube and punctures the sealing plug 33 in the connecting tube 31, so that the connecting tube 31 is communicated with the movable tube 32, and the reacted lysate in the sampling tube 21 is sucked into the vacuum tube 12 and reacts with the detection test paper 13.
The inner wall of the end of the movable tube 32 remote from the sampling tube 21 is provided with a sealing ring. The sealing ring is used to ensure the sealing between the movable tube 32 and the connection tube 31. The sealing ring adopts current rubber seal, and it has certain elasticity, conveniently rotates and presses movable tube 32, and can guarantee sealed effect simultaneously.
As shown in fig. 1, a safety ring 4 is disposed between the outer wall of the detection housing 11 and the outer wall of the movable tube 32 away from the sampling tube 21. Safety ring 4 adopts to connect between current bottle lid and uses safety ring, it adopts the plastic material, be used for guaranteeing to detect the card when not using, movable tube 32 is fixed with the outer wall that detects casing 11, it can not take place to rotate to have guaranteed between movable tube 32 and the connecting pipe 31, thereby avoid stinging stopper syringe needle 34 in the movable tube 32 to run through sealing plug 33 when not using, avoided unreacted lysate to pass through stinging stopper syringe needle 34, connecting pipe 31 enters into vacuum tube 12, avoid detecting the card condemned condition to appear.
In summary, when the test card is used, the sampling swab 23 is used to sample the sample, the sampled sample swab 23 is put into the sampling tube 21, the sealing cap 22 is tightened, under the action of the elastic sealing plug 221, the lysate in the sampling tube 21 will not overflow, at this time, the sample reacts with the lysate in the sampling tube 21, the safety ring 4 is then removed and the movable tube 32 is pressed or rotated so that the stopper needle 34 extends through the sealing plug 33 and into the connecting tube 31, whereupon, the reacted lysate in the sampling tube 21 of the sampling assembly 2 is sucked into the vacuum tube 12 due to the action of the vacuum tube 12, and contacting with a test paper 13, if the object to be detected contains the novel coronavirus, coupling the obtained antigen-antibody conjugate with enzyme, the combined product reacts with a corresponding substrate, and whether the combined product carries the new coronavirus is judged through the chromogenic reaction of the detection test paper 13; if the object to be detected does not contain the novel coronavirus, no conjugate is formed, and no color reaction occurs; the result of the color reaction can be observed in the visible area of the detection shell 11, and the operation is simple and convenient.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (7)
1. An enclosed test card for testing for new coronavirus, comprising: a detection assembly (1), a sampling assembly (2) adjacent to the detection assembly (1), and a closure assembly (3) disposed between the detection assembly (1) and the sampling assembly (2);
the detection assembly (1) comprises a detection shell (11) and a vacuum tube (12) longitudinally arranged in the detection shell (11), wherein detection test paper (13) is arranged in the vacuum tube (12), and a sealed transparent panel (14) corresponding to the vacuum tube (12) is arranged on the detection shell (11);
seal subassembly (3) including running through detect casing (11) and with connecting pipe (31) of vacuum tube (12) intercommunication, with sampling pipe (21) of sampling subassembly (2) are connected and with connecting pipe (31) swing joint's movable tube (32), be provided with sealing plug (33) in connecting pipe (31), be provided with in movable tube (32) with stinging stopper syringe needle (34) of sampling pipe (21) intercommunication, the drive movable tube (32) make stinging stopper syringe needle (34) puncture sealing plug (33) and make connecting pipe (31) with movable tube (32) intercommunication.
2. The closed detection card for detecting new coronavirus according to claim 1, wherein the sampling assembly (2) further comprises a sealing cover (22) detachably disposed at the end of the sampling tube (21), and a sampling swab (23) disposed inside the sealing cover (22), wherein a reaction layer (24) is disposed in the sampling tube (21), and the sampling swab (23) is axially disposed along the sampling tube (21) and extends into the reaction layer (24).
3. The closed detection card for the detection of new coronaviruses according to claim 2, characterized in that said sealing cap (22) is screwed with said sampling tube (21).
4. The closed detection card for the detection of new coronavirus according to claim 2, characterized in that the sealing cover (22) is provided on its inside with an elastic sealing plug (221) matching the inner diameter of the sampling tube (21), the sampling swab (23) passing through the elastic sealing plug (221) and being connected with the sealing cover (22).
5. The closed detection card for the detection of new coronaviruses according to claim 1, characterized in that the outer wall of the connection tube (31) is provided with an external thread and the inner wall of the movable tube (32) is provided with an internal thread which is engaged with the external thread of the outer wall of the connection tube (31).
6. The closed detection card for the detection of new coronavirus according to any one of claims 1 to 5, characterized in that the inner wall of the end of the movable tube (32) remote from the sampling tube (21) is provided with a sealing ring.
7. The closed detection card for the detection of new coronavirus according to claim 6, characterized in that a safety ring (4) is arranged between the outer wall of the detection case (11) and the outer wall of the movable tube (32) far from the sampling tube (21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210150777.0A CN114525202A (en) | 2022-02-18 | 2022-02-18 | Closed detection card for detecting new coronavirus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210150777.0A CN114525202A (en) | 2022-02-18 | 2022-02-18 | Closed detection card for detecting new coronavirus |
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| CN114525202A true CN114525202A (en) | 2022-05-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210150777.0A Pending CN114525202A (en) | 2022-02-18 | 2022-02-18 | Closed detection card for detecting new coronavirus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115902088A (en) * | 2023-03-09 | 2023-04-04 | 合肥泷睿机器人有限责任公司 | Device for testing virus killing performance of nano titanium dioxide photocatalytic screen |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN212207401U (en) * | 2020-04-22 | 2020-12-22 | 成都中珠健联基因科技有限责任公司 | Respiratory system infects integrative device of viroid sample collection detection |
| JP3233795U (en) * | 2021-06-25 | 2021-09-02 | 杭州安旭生物科技股▲分▼有限公司Ａｓｓｕｒｅ Ｔｅｃｈ． （Ｈａｎｇｚｈｏｕ） Ｃｏ．， ＬＴＤ | Sample collection and detection device |
| CN214636523U (en) * | 2020-11-23 | 2021-11-09 | 蔡竹 | Combined sample collection and detection device and sampling tube and detection tube thereof |
-
2022
- 2022-02-18 CN CN202210150777.0A patent/CN114525202A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN212207401U (en) * | 2020-04-22 | 2020-12-22 | 成都中珠健联基因科技有限责任公司 | Respiratory system infects integrative device of viroid sample collection detection |
| CN214636523U (en) * | 2020-11-23 | 2021-11-09 | 蔡竹 | Combined sample collection and detection device and sampling tube and detection tube thereof |
| JP3233795U (en) * | 2021-06-25 | 2021-09-02 | 杭州安旭生物科技股▲分▼有限公司Ａｓｓｕｒｅ Ｔｅｃｈ． （Ｈａｎｇｚｈｏｕ） Ｃｏ．， ＬＴＤ | Sample collection and detection device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115902088A (en) * | 2023-03-09 | 2023-04-04 | 合肥泷睿机器人有限责任公司 | Device for testing virus killing performance of nano titanium dioxide photocatalytic screen |
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