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CN114379912A - Immune cell product cryopreserving device - Google Patents

Immune cell product cryopreserving device Download PDF

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Publication number
CN114379912A
CN114379912A CN202210118613.XA CN202210118613A CN114379912A CN 114379912 A CN114379912 A CN 114379912A CN 202210118613 A CN202210118613 A CN 202210118613A CN 114379912 A CN114379912 A CN 114379912A
Authority
CN
China
Prior art keywords
liquid nitrogen
nitrogen tank
fixing
immune cell
storage box
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN202210118613.XA
Other languages
Chinese (zh)
Inventor
傅小聪
袁曾津
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Paisixin Biomedical Technology Co ltd
Original Assignee
Shanghai Paisixin Biomedical Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Paisixin Biomedical Technology Co ltd filed Critical Shanghai Paisixin Biomedical Technology Co ltd
Priority to CN202210118613.XA priority Critical patent/CN114379912A/en
Publication of CN114379912A publication Critical patent/CN114379912A/en
Withdrawn legal-status Critical Current

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D25/00Details of other kinds or types of rigid or semi-rigid containers
    • B65D25/02Internal fittings
    • B65D25/10Devices to locate articles in containers
    • B65D25/101Springs, elastic lips, or other resilient elements to locate the articles by pressure
    • A01N1/0242
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D25/00Details of other kinds or types of rigid or semi-rigid containers
    • B65D25/38Devices for discharging contents
    • B65D25/52Devices for discharging successive articles or portions of contents
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/18Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/18Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
    • B65D81/22Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient in moist conditions or immersed in liquids

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  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the field of cell freezing, and particularly relates to an immune cell product cryopreservation device which comprises a liquid nitrogen tank, wherein universal wheels are mounted on two sides of the bottom end of the liquid nitrogen tank through a support frame, a taking and placing opening is formed in one side of the top end of the liquid nitrogen tank, and a sealing cover is mounted on the taking and placing opening through a plurality of hasps; two sides of the inside of the liquid nitrogen tank are rotatably connected with vertically arranged rotating columns through bearing seats, chain wheels are mounted at the top ends of the rotating columns, a chain is connected between the two chain wheels, and a plurality of fixing mechanisms for storing cell products are uniformly mounted on the outer side walls of the chains; one rotates the post and is located get the below of putting the mouth, get one side of putting the mouth and be equipped with the telescopic link of installing on the liquid nitrogen container, the output shaft of telescopic link extends to the internal connection of liquid nitrogen container has one end to extend to the promotion piece of fixed establishment below. This device can be quick, solitary realization to getting of same batch of cell put, does not influence the sample of other storages, can not cause the damage of other samples.

Description

Immune cell product cryopreserving device
Technical Field
The invention relates to the technical field of cell freezing, in particular to a freezing storage device for immune cell products.
Background
A immune cell is a cell involved in or associated with an immune response. Including lymphocytes, dendritic cells, monocytes/macrophages, granulocytes, mast cells, and the like. Immune cells can be classified into various types, and various immune cells play an important role in the human body. Immune cells (immunecells) are commonly referred to as leukocytes, and include innate lymphocytes, various phagocytic cells, and the like, and lymphocytes that recognize antigens and generate specific immune responses, and the like.
The more and more products produced by using immune cells, the immune cell products are generally preserved in a freezing device to keep the activity of the immune cell products, a medical liquid nitrogen tank is a freezing device for storing a specimen, liquid nitrogen is filled in the liquid nitrogen tank, the specimen is frozen by means of the low temperature of the liquid nitrogen, and the storage and the transportation of the specimen are facilitated. During the use, partial shipment to freezing intraductal, mark sample information on freezing the pipe, will freeze and deposit the pipe again and put into the liquid nitrogen container. When a sample is taken and placed in an existing liquid nitrogen tank, a plurality of samples need to be taken at one time, the needed sample cannot be extracted independently, temperature rise damage which does not need to be extracted is caused easily, and therefore the immune cell product cryopreservation device is provided.
Disclosure of Invention
The invention provides a freezing device for immune cell products, which solves the problems in the background technology.
In order to achieve the purpose, the invention adopts the following technical scheme:
the device for freezing and storing the immune cell products comprises a liquid nitrogen tank, wherein universal wheels are mounted on two sides of the bottom end of the liquid nitrogen tank through supporting frames, a taking and placing opening is formed in one side of the top end of the liquid nitrogen tank, and a sealing cover is mounted on the taking and placing opening through a plurality of hasps;
two sides of the inside of the liquid nitrogen tank are rotatably connected with vertically arranged rotating columns through bearing seats, chain wheels are mounted at the top ends of the rotating columns, a chain is connected between the two chain wheels, and a plurality of fixing mechanisms for storing cell products are uniformly mounted on the outer side walls of the chains;
one rotates the post and is located get the below of putting the mouth, get one side of putting the mouth and be equipped with the telescopic link of installing on the liquid nitrogen container, the output shaft of telescopic link extends to the internal connection of liquid nitrogen container has one end to extend to the promotion piece of fixed establishment below.
It is worth explaining that a sliding groove is formed in the top end of the rotating column close to one side of the taking and placing opening, an extending rod is connected in the sliding groove in a sliding mode, a rotating handle is installed at the top end of the extending rod, and a second spring is connected between the bottom wall of the extending rod and the sliding groove.
It is worth explaining, fixed establishment is including fixing the fixed block on the chain lateral wall, and sliding connection has L type pole in the sliding hole on the fixed block, and the pull ring is installed on the top of L type pole, and the fixed column of vertical setting is installed to the bottom of L type pole, installs a plurality of holders through solid fixed ring on the fixed column.
It is worth explaining that, the holder includes the centre gripping frame that the cross-section is the E type, and the centre gripping frame is fixed in solid fixed ring's one side, has all opened the slip hole on the both sides wall of centre gripping frame, and the downthehole sliding connection of slip has the fixed pin, is connected with spring one between the one end of fixed pin and the outer wall of centre gripping frame, and the other end of fixed pin is connected with the grip block of sliding connection in centre gripping frame inside.
It is worth to say that, the holder still includes the connecting rod of fixing in centre gripping frame bottom, and the backup pad that is located centre gripping frame below is installed to the bottom of connecting rod.
It is worth explaining, storage box is installed to the bottom of liquid nitrogen container, and the intercommunicating pore has been opened to the junction intermediate position of storage box and liquid nitrogen container, and the inside rotation of storage box is connected with one end and extends to the outside two-way lead screw of storage box, and the equal threaded connection in both ends of two-way lead screw has the fly leaf of sliding seal connection in storage box inside.
It is worth mentioning that the side walls of the storage box and the liquid nitrogen tank are provided with vacuum interlayers.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention provides an immune cell product cryopreservation device which can be used for storing a plurality of samples of cells of a batch through a plurality of fixing mechanisms, and is convenient to distinguish and take independently;
through the setting of rotating post, sprocket, chain, telescopic link, promotion piece isotructure, can be quick, solitary realization is to getting of same batch of cell and is put, does not influence the sample of other storages, can not cause the damage of other samples.
2. According to the immune cell product cryopreservation device, due to the arrangement of the structures such as the communication hole, the storage box, the bidirectional screw rod and the movable plate, when the amount of liquid nitrogen in the liquid nitrogen tank is insufficient, the liquid level line can be quickly lifted under the condition that the taking and placing port does not need to be opened, and the condition that the liquid nitrogen is volatilized due to frequent opening of the taking and placing port is reduced.
Drawings
FIG. 1 is a schematic structural diagram of an apparatus for cryopreserving an immune cell product according to the present invention;
FIG. 2 is a cross-sectional view of an apparatus for cryopreserving an immune cell product according to the present invention;
FIG. 3 is a schematic top view of the device for cryopreserving an immune cell product according to the present invention, with the sealing cap opened;
FIG. 4 is a schematic diagram of a storage box and the internal structure of a cryopreservation device for immune cell products according to the present invention;
FIG. 5 is a schematic partial structure diagram of a cryopreservation apparatus for an immune cell product according to the present invention;
FIG. 6 is a schematic structural view of the securing mechanism of FIG. 5;
fig. 7 is a schematic structural view of the clamping member in fig. 6.
In the figure: 1. a storage box; 2. a universal wheel; 3. a support frame; 4. a liquid nitrogen tank; 401. a communicating hole; 5. a hasp; 6. a sealing cover; 7. a telescopic rod; 8. a taking and placing port; 9. rotating the column; 10. a sprocket; 11. a chain; 12. a lifting block; 13. a bidirectional screw rod; 14. a movable plate; 15. an extension rod; 16. rotating the handle; 17. a fixing mechanism; 1701. a pull ring; 1702. an L-shaped rod; 1703. a fixed block; 1704. a clamping member; 17041. a support plate; 17042. a connecting rod; 17043. a fixing pin; 17044. a first spring; 17045. a clamping plate; 17046. a clamping frame; 1705. a fixing ring; 1706. and (5) fixing the column.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-7, an immune cell product cryopreservation device comprises a liquid nitrogen tank 4, wherein universal wheels 2 are mounted on two sides of the bottom end of the liquid nitrogen tank 4 through support frames 3, the universal wheels 2 are arranged to facilitate transfer of the device, a taking and placing opening 8 is formed in one side of the top end of the liquid nitrogen tank 4, a sealing cover 6 is mounted on the taking and placing opening 8 through a plurality of hasps 5, the taking and placing opening 8 is smaller than the liquid nitrogen tank 4, and volatilization of liquid nitrogen during opening is reduced;
two sides of the inside of the liquid nitrogen tank 4 are rotatably connected with vertically arranged rotating columns 9 through bearing seats, chain wheels 10 are mounted at the top ends of the rotating columns 9, a chain 11 is connected between the two chain wheels 10, and a plurality of fixing mechanisms 17 for storing cell products are uniformly mounted on the outer side wall of the chain 11;
one rotates the post 9 and is located get the below of putting mouth 8, get one side of putting mouth 8 and be equipped with telescopic link 7 of installing on liquid nitrogen container 4, the output shaft of telescopic link 7 extends to the internal connection of liquid nitrogen container 4 has one end to extend to the lifting block 12 of fixed establishment 17 below.
It has the spout to be close to getting to open on 9 tops of the rotation post of putting mouth 8 one side, sliding connection has extension rod 15 in the spout, extension rod 15's cross-section is the rectangle, can drive rotation post 9 rotatoryly, turning handle 16 is installed on extension rod 15's top, and be connected with spring two between extension rod 15's diapire and the spout, press extension rod 15 through sealed lid 6 at ordinary times, press it inside liquid nitrogen tank 4, opening sealed lid 6 after, under spring two effects, bounce automatically.
As shown in fig. 6, the fixing mechanism 17 includes a fixing block 1703 fixed on the outer side wall of the chain 11, an L-shaped rod 1702 is slidably connected in a sliding hole on the fixing block 1703, a pull ring 1701 is installed at the top end of the L-shaped rod 1702, a number label with a noctilucent display function is provided on each pull ring 1701, which is convenient for finding a required specimen, a vertically arranged fixing column 1706 is installed at the bottom end of the L-shaped rod 1702, a plurality of clamping members 1704 are installed on the fixing column 1706 through a fixing ring 1705, and when the fixing column 1706 is not lifted at ordinary times, under the action of gravity, the L-shaped rod 1702 drives a connecting structure and a freezing pipe thereon to be slidably connected on the fixing block 1703.
As shown in fig. 7, the clamping member 1704 includes a clamping frame 17046 having an E-shaped cross section, the clamping frame 17046 is fixed on one side of the fixing ring 1705, two side walls of the clamping frame 17046 are respectively provided with a sliding hole, a fixing pin 17043 is slidably connected in the sliding hole, a first spring 17044 is connected between one end of the fixing pin 17043 and an outer wall of the clamping frame 17046, the other end of the fixing pin 17043 is connected with a clamping plate 17045 slidably connected inside the clamping frame 17046, and one clamping member 1704 can clamp two freezing tubes at a time.
Further, the clamping member 1704 further includes a connecting rod 17042 fixed at the bottom end of the clamping frame 17046, and the bottom end of the connecting rod 17042 is provided with a supporting plate 17041 located below the clamping frame 17046, so as to provide a supporting function for the freezing pipe clamped in the clamping frame 17046, and improve the clamping stability of the freezing pipe.
As shown in fig. 1, 2, 4, storage box 1 is installed to the bottom of liquid nitrogen container 4, intercommunication hole 401 has been opened to storage box 1 and the junction intermediate position of liquid nitrogen container 4, the internal rotation of storage box 1 is connected with one end and extends to the outside two-way lead screw 13 of storage box 1, the equal threaded connection in both ends of two-way lead screw 13 has the fly leaf 14 of sliding seal connection in storage box 1 inside, the inside of storage box 1 stores the liquid nitrogen equally, setting through fly leaf 14, have the function of adjusting the inside liquid level height of liquid nitrogen container 4, the lateral wall of storage box 1 and liquid nitrogen container 4 all is equipped with the vacuum interlayer, improve the heat preservation effect of device.
When a stored sample needs to be taken, the hasp 5 on the sealing cover 6 is loosened, the sealing cover 6 is taken down, under the action of the spring II, the extension rod 15 on the rotating column 9 drives the rotating handle 16 to move upwards to the position above the taking and placing opening 8, the rotating handle 16 is rotated to drive the rotating column 9 to rotate, the rotating column 9 drives the plurality of fixing mechanisms 17 on the chain 11 to rotate in the liquid nitrogen tank 4 through the chain wheel 10, different fixing mechanisms 17 are continuously driven to move to the position below the taking and placing opening 8, the sample needing to be found is found through the digital identification on the pull ring 1701, when the fixing mechanism 17 with the stored specified sample moves to the position above the lifting block 12, the output shaft of the telescopic rod 7 drives the L-shaped rod 1702 to move upwards on the fixing block 1703, the clamping piece 1704 and the sample clamped thereon are driven to extend to the taking and placing opening 8, then the fixing pin 17043 is driven to drive the spring 17044 to stretch, the clamping plate 17045 is driven to not to clamp the tube body containing the sample any more, the specimen can be taken down, so that the required specimen can be taken down according to the requirement;
when liquid nitrogen liquid level is not enough in the liquid nitrogen guide tank 4 in needs inspection, rotate two-way lead screw 13, two-way lead screw 13 and two fly leaf 14 threaded connection drive the liquid nitrogen in the storage box 1 constantly to flow to liquid nitrogen tank 4 in through intercommunicating pore 401, can get under the condition of putting mouthful 8 in need not opening, promote the liquid level line fast, reduced frequently open get put mouthful lead to the volatile condition of liquid nitrogen.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (7)

1. The device for freezing and storing the immune cell products comprises a liquid nitrogen tank (4) and is characterized in that universal wheels (2) are mounted on two sides of the bottom end of the liquid nitrogen tank (4) through a support frame (3), a taking and placing opening (8) is formed in one side of the top end of the liquid nitrogen tank (4), and a sealing cover (6) is mounted on the taking and placing opening (8) through a plurality of hasps (5);
two sides in the liquid nitrogen tank (4) are rotatably connected with vertically arranged rotating columns (9) through bearing seats, chain wheels (10) are mounted at the top ends of the rotating columns (9), a chain (11) is connected between the two chain wheels (10), and a plurality of fixing mechanisms (17) for storing cell products are uniformly mounted on the outer side wall of the chain (11);
one rotates post (9) and is located get the below of putting mouth (8), get one side of putting mouth (8) and be equipped with telescopic link (7) of installing on liquid nitrogen container (4), the output shaft of telescopic link (7) extends to the internal connection of liquid nitrogen container (4) and has one end to extend to hoist block (12) of fixed establishment (17) below.
2. The device for freezing and storing the immune cell products as claimed in claim 1, wherein a sliding groove is formed at the top end of the rotating column (9) near one side of the access opening (8), an extending rod (15) is connected in the sliding groove in a sliding manner, a rotating handle (16) is installed at the top end of the extending rod (15), and a second spring is connected between the bottom wall of the extending rod (15) and the sliding groove.
3. The device for cryopreserving the immune cell products, according to claim 1, wherein the fixing mechanism (17) comprises a fixing block (1703) fixed on the outer side wall of the chain (11), an L-shaped rod (1702) is connected in a sliding hole in the fixing block (1703) in a sliding manner, a pull ring (1701) is installed at the top end of the L-shaped rod (1702), a vertically arranged fixing column (1706) is installed at the bottom end of the L-shaped rod (1702), and a plurality of clamping pieces (1704) are installed on the fixing column (1706) through a fixing ring (1705).
4. The device for cryopreserving an immune cell product, according to claim 3, wherein the clamping member (1704) comprises a clamping frame (17046) with an E-shaped cross section, the clamping frame (17046) is fixed on one side of the fixing ring (1705), sliding holes are formed in two side walls of the clamping frame (17046), a fixing pin (17043) is connected in the sliding holes in a sliding mode, a first spring (17044) is connected between one end of the fixing pin (17043) and the outer wall of the clamping frame (17046), and a clamping plate (17045) connected in the clamping frame (17046) in a sliding mode is connected to the other end of the fixing pin (17043).
5. The cryopreservation device of claim 4, wherein the clamping member (1704) further comprises a connecting rod (17042) fixed at the bottom end of the clamping frame (17046), and the bottom end of the connecting rod (17042) is provided with a support plate (17041) positioned below the clamping frame (17046).
6. The immune cell product cryopreservation device according to claim 1, wherein a storage box (1) is installed at the bottom end of the liquid nitrogen tank (4), a communication hole (401) is formed in the middle position of the connection position of the storage box (1) and the liquid nitrogen tank (4), a bidirectional screw rod (13) with one end extending to the outside of the storage box (1) is rotatably connected to the inside of the storage box (1), and movable plates (14) which are connected to the inside of the storage box (1) in a sliding and sealing mode are in threaded connection at two ends of the bidirectional screw rod (13).
7. The cryopreservation device for immune cell products according to claim 6, wherein the side walls of the storage box (1) and the liquid nitrogen tank (4) are provided with vacuum interlayers.
CN202210118613.XA 2022-02-08 2022-02-08 Immune cell product cryopreserving device Withdrawn CN114379912A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210118613.XA CN114379912A (en) 2022-02-08 2022-02-08 Immune cell product cryopreserving device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210118613.XA CN114379912A (en) 2022-02-08 2022-02-08 Immune cell product cryopreserving device

Publications (1)

Publication Number Publication Date
CN114379912A true CN114379912A (en) 2022-04-22

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210118613.XA Withdrawn CN114379912A (en) 2022-02-08 2022-02-08 Immune cell product cryopreserving device

Country Status (1)

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CN (1) CN114379912A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118651546A (en) * 2024-08-19 2024-09-17 阳信华胜清真肉类有限公司 Meat product fresh-keeping storage equipment

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118651546A (en) * 2024-08-19 2024-09-17 阳信华胜清真肉类有限公司 Meat product fresh-keeping storage equipment
CN118651546B (en) * 2024-08-19 2024-10-22 阳信华胜清真肉类有限公司 Meat product fresh-keeping storage equipment

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Application publication date: 20220422

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