CN114107009B - Cell pipetting gun - Google Patents
Cell pipetting gun Download PDFInfo
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- CN114107009B CN114107009B CN202111325746.6A CN202111325746A CN114107009B CN 114107009 B CN114107009 B CN 114107009B CN 202111325746 A CN202111325746 A CN 202111325746A CN 114107009 B CN114107009 B CN 114107009B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The application provides a cell pipette, and relates to the technical field of biology. The scheme of the application is as follows: a cell pipette comprises a pipette body, a pipette body and a compression structure; the top end of the pipette cylinder body is provided with a compression structure for compressing air in the pipette cylinder body; the bottom of the pipette gun cylinder body is connected with a straw body communicated with the inside of the pipette gun cylinder body; an exhaust assembly for exhausting air in the pipette barrel is arranged at the top of the pipette barrel; the pipette body is provided with a blocking component which is used for isolating the pipette barrel from outside air. The application can pump cell mixed liquid with full range.
Description
Technical Field
The application relates to the technical field of biology, in particular to a cell pipette.
Background
Cell culture is also known as cell cloning technology, a formal biological term being cell culture technology. Cell culture is an essential process for the whole biotechnology, or for one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technique can be a simple single cell or a few differentiated multicellular from a single cell through mass culture, which is an essential link of the cloning technique, and the cell culture itself is the cloning of the cell.
At present, when a cell mixed solution is extracted by a liquid-transferring gun, gas exists in the cell mixed solution, when the cell mixed solution is extracted into a culture solution by a liquid-transferring gun cylinder body, the pressure difference is utilized to enable the cell mixed solution to enter the interior of the liquid-transferring gun cylinder body, the pressure in the culture solution is reduced in the extraction process, and part of gas in the culture solution is separated out due to the problem of pressure reduction, so that the cell mixed solution with full range cannot be extracted in the liquid-transferring gun.
Disclosure of Invention
The application aims to provide a cell pipetting gun which can pump cell mixed liquid with full range.
Embodiments of the present application are implemented as follows:
the embodiment of the application provides a cell pipette, which comprises a pipette cylinder body, a pipette body and a compression structure; the top end of the pipette cylinder body is provided with a compression structure for compressing air in the pipette cylinder body; the bottom of the pipette gun cylinder body is connected with a straw body communicated with the inside of the pipette gun cylinder body; an exhaust assembly for exhausting air in the pipette barrel is arranged at the top of the pipette barrel; the pipette body is provided with a blocking component which is used for isolating the pipette barrel from outside air.
In some embodiments of the present application, the compression structure includes a push-pull rod and a compression base plate, the push-pull rod penetrates through the top of the pipette barrel, one end of the push-pull rod is connected with the compression base plate, the compression base plate is located in the pipette barrel, and the annular side of the compression base plate is attached to the inner side wall of the pipette barrel.
In some embodiments of the present application, an elastic rubber ring is sleeved on the ring side of the compression substrate.
In some embodiments of the present application, a return spring is installed between the compression base plate and the pipette barrel, one end of the return spring is connected to the compression base plate, and the other end of the return spring is connected to the inner upper wall of the pipette barrel.
In some embodiments of the present application, the exhaust assembly includes a rotating base plate and a first rotating member for rotating the rotating base plate, the rotating base plate is rotationally connected with the compressing base plate, the compressing base plate is provided with a first air vent, the rotating base plate is provided with a second air vent matched with the first air vent, and the top of the pipette barrel is provided with an arc sliding port matched with the first rotating member.
In some embodiments of the present application, an elastic rubber ring is sleeved on the ring side of the rotating substrate.
In some embodiments of the present application, the locking assembly includes a locking housing and a roller, the straw body penetrates the locking housing, the locking housing is provided with a rolling groove matched with the roller, both sides of the rolling groove are provided with connecting grooves matched with the roller, and one end of the connecting groove is higher than the other end of the connecting groove.
In some embodiments of the application, a viewing window is mounted on the side wall of the pipette barrel.
In some embodiments of the present application, the observation window is provided with graduation marks from top to bottom.
Compared with the prior art, the embodiment of the application has at least the following advantages or beneficial effects:
a cell pipette comprises a pipette body, a pipette body and a compression structure; the top end of the pipette cylinder body is provided with a compression structure for compressing air in the pipette cylinder body; the bottom of the pipette gun cylinder body is connected with a straw body communicated with the inside of the pipette gun cylinder body; an exhaust assembly for exhausting air in the pipette barrel is arranged at the top of the pipette barrel; the pipette body is provided with a blocking component which is used for isolating the pipette barrel from outside air.
In the above embodiment, a cell pipette comprises a pipette body, a pipette body and a compression structure, wherein the compression structure is installed at the upper part of the pipette body, the pipette body is installed at the lower part of the pipette body, the compression structure can be used for compressing air in the pipette body, in particular, when a worker extracts a cell mixed solution in a culture solution, firstly, the air in the pipette body is discharged through the compression structure, then the pipette body at the bottom of the pipette body is placed in the culture solution, then negative pressure is generated in the pipette body through the compression structure, the pipette body sucks a cell mixed solution into the culture solution through the pipette body, on one hand, because gas exists in the cell mixed solution, when the pipette body sucks the cell mixed solution into the culture solution, the pressure difference is utilized to enable the cell mixed solution to enter the inside of the pipette body, the pressure in the extraction process is reduced, and part of the gas in the culture solution is separated out because of the problem of pressure reduction, on the second aspect, when the pipette body extracts the culture solution into the culture solution, the culture solution is hard to be caused, the pipette body is still in the same, the suction efficiency is not generated, and then the cell mixed solution is not in the culture solution is extracted into the culture solution, and the cell mixed solution is left in the culture solution is in the middle of the three aspects, and the residual problem exists in the cell mixed solution can be extracted after the cell mixed solution is extracted into the culture solution, and the cell mixed solution is in the culture solution is in the medium, and the three aspects of the condition that the cell mixed solution is in the medium, and the cell mixed solution is in the cell solution is in the culture solution is in the medium, and the cell solution is in the cell solution, if there is unnecessary air in the pipette barrel, discharge the outside to the pipette barrel through exhaust assembly with above-mentioned unnecessary air, at this in-process staff opens and seals the subassembly and prevent that the cell mixture in the pipette barrel from flowing into outside from the straw body, the staff opens the compression structure once more when the whole outside of discharging of air in the pipette barrel, make the pipette barrel again to the cell mixture of the same volume of culture solution extraction discharge unnecessary air, make the pipette barrel in take out full cell mixture, inside have had the unnecessary air to cause the problem that the pipette barrel work efficiency is low after having avoided the pipette barrel to extract cell mixture.
In this embodiment, when the staff extracts the cell mixed solution into the culture solution through the cell pipette, the pressure difference of air is utilized to extract the cell mixed solution into the pipette barrel, on one hand, because gas exists in the cell mixed solution, the pressure difference is utilized to enable the cell mixed solution to enter the inside of the pipette barrel when the cell mixed solution is extracted into the culture solution, the pressure in the culture solution becomes small in the extraction process, part of gas in the culture solution is separated out because of the problem of pressure reduction, on the other hand, the pipette barrel is too violent when extracting into the culture solution, so that the culture solution does not enter the pipette barrel in time to generate a transient vacuum, and then disappears, on the other hand, the problem that residual gas exists before the pipette barrel extracts the cell mixed solution into the culture solution, the unnecessary gas exists in the pipette barrel and the cell mixed solution cannot be fully extracted, the staff can drain the unnecessary gas in the pipette barrel through an exhaust assembly, and then the compressed structure enables the pipette barrel to extract the same volume of cell mixed solution again, and the working efficiency of the cell mixed solution can be improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a cell pipette according to an embodiment of the present application;
FIG. 2 is a schematic view of the interior of a pipette barrel according to an embodiment of the present application;
FIG. 3 is a schematic view of the upper end structure of a pipette barrel according to an embodiment of the present application;
FIG. 4 is a partial cross-sectional view of a cell pipette according to an embodiment of the present application.
The figures indicate: 1-a liquid-transferring gun cylinder body, 2-a push-pull rod, 3-a straw body, 4-a sealing component, 5-a first rotating piece, 6-a rotating base plate, 7-a compressing base plate, 8-a reset spring, 9-a second air vent, 10-a first air vent, 11-an arc sliding port, 12-a first connecting section and 13-a second connecting section.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments of the present application. The components of the embodiments of the present application generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the application, as presented in the figures, is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the embodiments of the present application, it should be noted that, if the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate an azimuth or a positional relationship based on that shown in the drawings, or an azimuth or a positional relationship in which the product of the present application is conventionally put when used, it is merely for convenience of describing the present application and simplifying the description, and it does not indicate or imply that the apparatus or element to be referred to must have a specific azimuth, be configured and operated in a specific azimuth, and thus should not be construed as limiting the present application. Furthermore, the terms "first," "second," and the like, are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
Furthermore, the terms "horizontal," "vertical," "overhang" and the like, if any, do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the embodiments of the present application, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" should be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art according to the specific circumstances.
Examples
Please refer to fig. 1-3. The embodiment provides a cell pipette, which comprises a pipette cylinder body 1, a pipette body 3 and a compression structure; the top end of the pipette cylinder body 1 is provided with a compression structure for compressing air in the pipette cylinder body 1; the bottom of the pipette barrel 1 is connected with a straw body 3 communicated with the inside of the pipette barrel 1; an exhaust component for exhausting air in the pipette gun barrel 1 is arranged at the top of the pipette gun barrel 1; the pipette body 3 is provided with a blocking component 4 for isolating the inside of the pipette barrel 1 from the outside air.
In the above embodiment, a cell pipette consists of a pipette body 1, a pipette body 3 and a compression structure, wherein the compression structure is installed at the upper part of the pipette body 1, the pipette body 3 is installed at the lower part of the pipette body 1, the compression structure can be used for compressing air in the pipette body 1, specifically, when a worker extracts a cell mixture in a culture solution, firstly, the air in the pipette body 1 is discharged through the compression structure, then the pipette body 3 at the bottom of the pipette body 1 is placed in the culture solution, then, negative pressure is generated in the pipette body 1 through the compression structure, the pipette body 1 sucks the cell mixture into the culture solution through the pipette body 3, on one hand, because gas exists in the cell mixture, when the pipette body 1 sucks the cell mixture into the culture solution, the pressure difference is utilized to enable the cell mixture to enter the inside of the pipette body 1, in the process of extracting, the pressure in the culture solution becomes small, part of the gas in the culture solution is precipitated because of the problem of pressure reduction, in the second aspect, when the liquid-transferring gun barrel 1 is extracted into the culture solution, the culture solution does not enter the liquid-transferring gun barrel 1 in time to generate short vacuum and then disappears, in the third aspect, residual gas exists before the liquid-transferring gun barrel 1 extracts the cell mixed solution into the culture solution, the above problems can lead the staff to extract the cell mixed solution into the liquid-transferring gun barrel 1, and then redundant gas exists in the liquid-transferring gun barrel 1, and the redundant gas can not be discharged and then the cell mixed solution with the same volume can be extracted into the culture solution, thus the problem of low working efficiency is caused, if the redundant air exists in the pipette barrel 1, the redundant air is discharged to the outside of the pipette barrel 1 through the exhaust assembly, in the process, the worker opens the blocking assembly 4 to prevent the cell mixture in the pipette barrel 1 from flowing into the outside from the suction pipe body 3, and when the air in the pipette barrel 1 is completely discharged to the outside, the worker opens the compression structure again to enable the pipette barrel 1 to extract and discharge the cell mixture with the same volume of the redundant air to the culture solution, so that the cell mixture is fully extracted in the pipette barrel 1, and the problem that the working efficiency of the pipette barrel 1 is low due to the fact that the redundant air exists in the pipette barrel 1 after the cell mixture is extracted is avoided.
In this embodiment, when the staff extracts the cell mixture into the culture solution through the cell pipette, the pressure difference of air is used to extract the cell mixture into the pipette barrel 1, on one hand, because the gas exists in the cell mixture, the pressure difference is used to make the cell mixture enter the inside of the pipette barrel 1 when the cell mixture is extracted into the culture solution by the pipette barrel 1, the pressure in the culture solution becomes small in the extraction process, part of the gas in the culture solution is separated out because of the problem of pressure reduction, on the other hand, the pipette barrel 1 is too violent when extracting the culture solution, so that the culture solution does not enter the pipette barrel 1 in time to generate a short vacuum, and then disappears, on the other hand, the problem that the residual gas exists before the pipette barrel 1 extracts the cell mixture into the culture solution, the residual gas exists in the pipette barrel 1, so that the residual gas exists in the pipette barrel 1 and cannot be pumped into the cell mixture after the pipette barrel 1, the residual gas in the pipette barrel 1 can be discharged through the exhaust assembly, and then the residual gas in the pipette barrel 1 is discharged through the compression structure, so that the residual gas in the pipette barrel 1 can be discharged into the culture solution, and the cell mixture can be more efficiently extracted for more than the cell mixture.
In some embodiments of the present application, the compression structure includes a push-pull rod 2 and a compression base plate 7, the push-pull rod 2 penetrates through the top of the pipette barrel 1, one end of the push-pull rod 2 is connected with the compression base plate 7, the compression base plate 7 is located in the pipette barrel 1, and the annular side of the compression base plate 7 is attached to the inner side wall of the pipette barrel 1.
In this embodiment, the compression structure is composed of a push-pull rod 2 and a compression substrate 7, one end of the push-pull rod 2 is connected with the compression substrate 7, specifically, firstly, a worker pushes the push-pull rod 2 downwards to discharge air in the pipette barrel 1, and then pulls the push-pull rod 2 upwards to extract the cell mixture in the culture solution.
In some embodiments of the application, the annular side of the compression base 7 is sleeved with an elastic rubber ring.
In this embodiment, the annular side of the compression substrate 7 is sleeved with an elastic rubber ring, and the elastic rubber ring can isolate air between the compression substrate 7 and the inner side wall of the pipette barrel 1, so that the failure problem of work is avoided.
In some embodiments of the application, a return spring 8 is mounted between the compression base 7 and the pipette cylinder 1, one end of the return spring 8 is connected to the compression base 7, and the other end of the return spring 8 is connected to the inner upper wall of the pipette cylinder 1.
In this embodiment, one end of the return spring 8 is connected with the compression substrate 7, the other end of the return spring 8 is connected with the inner upper wall of the pipette barrel 1, and after the staff pushes the push-pull rod 2 downwards to discharge the air in the pipette barrel 1, the compression substrate 7 moves upwards under the action of the return spring 8 to enable the pipette barrel 1 to form a pressure difference with the outside, so that the cell mixed solution can be sucked.
In some embodiments of the application, the air vent assembly comprises a rotating base plate 6 and a first rotating member 5 for rotating the rotating base plate 6, the rotating base plate 6 is rotatably connected with a compressing base plate 7, the compressing base plate 7 is provided with a first air vent 10, the rotating base plate 6 is provided with a second air vent 9 matched with the first air vent 10, and the top of the pipette gun barrel 1 is provided with an arc-shaped sliding opening 11 matched with the first rotating member 5.
In this embodiment, the exhaust assembly is composed of a rotating base plate 6 and a first rotating member 5, the rotating base plate 6 is rotationally connected with a compressing base plate 7, specifically, when a worker needs to exhaust the redundant gas in the pipette barrel 1 when extracting the cell mixture, the worker slides the first rotating member 5 along the direction of the arc sliding port 11, closes the sealing assembly 4 when aligning the first air port 10 of the compressing base plate 7 with the second air port 9 of the rotating base plate 6, and then moves the compressing base plate 7 downwards to sequentially exhaust the redundant gas from the first air port 10, the second air port 9 and the arc sliding port 11.
In some embodiments of the application, the annular side of the rotating base plate 6 is sleeved with an elastic rubber ring.
In this embodiment, the annular side of the rotary base plate 6 is sleeved with an elastic rubber ring to isolate the air between the rotary base plate 6 and the inner side wall of the pipette barrel 1.
In some embodiments of the present application, the locking assembly 4 comprises a locking housing and a roller, the straw body 3 penetrates the locking housing, the locking housing is provided with a rolling groove matched with the roller, both sides of the rolling groove are provided with connecting grooves matched with the roller, and the level of one end of the connecting groove is higher than the level of the other end of the connecting groove.
In this embodiment, the locking assembly 4 comprises locking shell and gyro wheel, makes the gyro wheel to the low one end of spread groove slide when the staff closes straw body 3, makes gyro wheel extrusion straw body 3 avoid the cell mixed liquor in the pipette barrel 1 to flow, makes the gyro wheel to the high one end of spread groove slide when the staff opens straw body 3, avoids gyro wheel extrusion straw body 3, can carry out the work of extracting cell mixed liquor.
In some embodiments of the application, the side wall of the pipette barrel 1 is fitted with a viewing window.
In this embodiment, the operator can observe the situation in the pipette barrel 1 in real time through the observation window, and can perform actions at any time.
In some embodiments of the application, the viewing window is provided with graduation marks from top to bottom.
In this embodiment, the staff can obviously know the amount of the cell mixture in the pipette barrel 1 through the scale marks.
Please refer to fig. 4. In some embodiments of the present application, the pipette body 1 is composed of a first connecting section 12 and a second connecting section 13, wherein the first connecting section 12 is detachably connected with the second connecting section 13 (the outer side wall of the second connecting section 13 is provided with threads, the inner side wall of the first connecting section 12 is provided with threads, the second connecting section 13 is in communication with the first connecting section 12 through the threads), specifically, after the pipetting of the staff is completed, the cell sap is filled in the second connecting section 13, then the first connecting section 12 and the second connecting section 13 are relatively rotated, so that the first connecting section 12 and the second connecting section 13 are separated, and then the other second connecting section 13 is replaced for the next pipetting operation, and since the side walls of the rotating base plate 6 and the compressing base plate 7 are sleeved with elastic rubber, the side walls of the second connecting section 13 can be extruded between the first connecting section 12 and the rotating base plate 6 and the compressing base plate 7 when the second connecting section 13 is connected with the first connecting section 12, and the pipetting operation can be smoothly performed.
In summary, embodiments of the present application provide a cell pipette, including a pipette body 1, a pipette body 3, and a compression structure; the top end of the pipette cylinder body 1 is provided with a compression structure for compressing air in the pipette cylinder body 1; the bottom of the pipette barrel 1 is connected with a straw body 3 communicated with the inside of the pipette barrel 1; an exhaust component for exhausting air in the pipette gun barrel 1 is arranged at the top of the pipette gun barrel 1; the pipette body 3 is provided with a blocking component 4 for isolating the inside of the pipette barrel 1 from the outside air.
In the above embodiment, a cell pipette consists of a pipette body 1, a pipette body 3 and a compression structure, wherein the compression structure is installed at the upper part of the pipette body 1, the pipette body 3 is installed at the lower part of the pipette body 1, the compression structure can be used for compressing air in the pipette body 1, specifically, when a worker extracts a cell mixture in a culture solution, firstly, the air in the pipette body 1 is discharged through the compression structure, then the pipette body 3 at the bottom of the pipette body 1 is placed in the culture solution, then, negative pressure is generated in the pipette body 1 through the compression structure, the pipette body 1 sucks the cell mixture into the culture solution through the pipette body 3, on one hand, because gas exists in the cell mixture, when the pipette body 1 sucks the cell mixture into the culture solution, the pressure difference is utilized to enable the cell mixture to enter the inside of the pipette body 1, in the process of extracting, the pressure in the culture solution becomes small, part of the gas in the culture solution is precipitated because of the problem of pressure reduction, in the second aspect, when the liquid-transferring gun barrel 1 is extracted into the culture solution, the culture solution does not enter the liquid-transferring gun barrel 1 in time to generate short vacuum and then disappears, in the third aspect, residual gas exists before the liquid-transferring gun barrel 1 extracts the cell mixed solution into the culture solution, the above problems can lead the staff to extract the cell mixed solution into the liquid-transferring gun barrel 1, and then redundant gas exists in the liquid-transferring gun barrel 1, and the redundant gas can not be discharged and then the cell mixed solution with the same volume can be extracted into the culture solution, thus the problem of low working efficiency is caused, if the redundant air exists in the pipette barrel 1, the redundant air is discharged to the outside of the pipette barrel 1 through the exhaust assembly, in the process, the worker opens the blocking assembly 4 to prevent the cell mixture in the pipette barrel 1 from flowing into the outside from the suction pipe body 3, and when the air in the pipette barrel 1 is completely discharged to the outside, the worker opens the compression structure again to enable the pipette barrel 1 to extract and discharge the cell mixture with the same volume of the redundant air to the culture solution, so that the cell mixture is fully extracted in the pipette barrel 1, and the problem that the working efficiency of the pipette barrel 1 is low due to the fact that the redundant air exists in the pipette barrel 1 after the cell mixture is extracted is avoided.
In this embodiment, when the staff extracts the cell mixture into the culture solution through the cell pipette, the pressure difference of air is used to extract the cell mixture into the pipette barrel 1, on one hand, because the gas exists in the cell mixture, the pressure difference is used to make the cell mixture enter the inside of the pipette barrel 1 when the cell mixture is extracted into the culture solution by the pipette barrel 1, the pressure in the culture solution becomes small in the extraction process, part of the gas in the culture solution is separated out because of the problem of pressure reduction, on the other hand, the pipette barrel 1 is too violent when extracting the culture solution, so that the culture solution does not enter the pipette barrel 1 in time to generate a short vacuum, and then disappears, on the other hand, the problem that the residual gas exists before the pipette barrel 1 extracts the cell mixture into the culture solution, the residual gas exists in the pipette barrel 1, so that the residual gas exists in the pipette barrel 1 and cannot be pumped into the cell mixture after the pipette barrel 1, the residual gas in the pipette barrel 1 can be discharged through the exhaust assembly, and then the residual gas in the pipette barrel 1 is discharged through the compression structure, so that the residual gas in the pipette barrel 1 can be discharged into the culture solution, and the cell mixture can be more efficiently extracted for more than the cell mixture.
The above is only a preferred embodiment of the present application, and is not intended to limit the present application, but various modifications and variations can be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.
Claims (6)
1. The cell pipette is characterized by comprising a pipette barrel, a pipette body and a compression structure;
the top end of the pipette barrel is provided with a compression structure for compressing air in the pipette barrel;
the bottom of the pipette gun cylinder body is connected with the pipette body communicated with the inside of the pipette gun cylinder body;
an exhaust assembly for exhausting air in the pipette barrel is arranged at the top of the pipette barrel;
the pipette body is provided with a blocking assembly for isolating the pipette barrel from outside air;
the compression structure comprises a push-pull rod and a compression substrate, the push-pull rod penetrates through the top of the pipette barrel, one end of the push-pull rod is connected with the compression substrate, the compression substrate is positioned in the pipette barrel, and the annular side of the compression substrate is attached to the inner side wall of the pipette barrel;
the exhaust assembly comprises a rotating base plate and a first rotating piece used for rotating the rotating base plate, the rotating base plate is rotationally connected with the compression base plate, the compression base plate is provided with a first air vent, the rotating base plate is provided with a second air vent matched with the first air vent, and the top of the pipette gun barrel is provided with an arc sliding port matched with the first rotating piece;
the sealing assembly comprises a sealing shell and a roller, wherein the sealing shell penetrates through the suction pipe body, the sealing shell is provided with a rolling groove matched with the roller, two sides of the rolling groove are respectively provided with a connecting groove matched with the roller, and the horizontal height of one end of the connecting groove is higher than that of the other end of the connecting groove.
2. The cell pipette of claim 1 wherein the annular side of the compression base plate is provided with an elastomeric rubber ring.
3. The cell pipette of claim 1 wherein a return spring is mounted between said compression base and said pipette barrel, one end of said return spring being connected to said compression base and the other end of said return spring being connected to the inner upper wall of said pipette barrel.
4. The cell pipette of claim 1 wherein the annular side of the rotating base plate is provided with an elastomeric rubber ring.
5. The cell pipette of claim 1 wherein a side wall of the pipette barrel is fitted with a viewing window.
6. The cell pipette of claim 5 wherein said viewing window is provided with graduation lines from top to bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111325746.6A CN114107009B (en) | 2021-11-10 | 2021-11-10 | Cell pipetting gun |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111325746.6A CN114107009B (en) | 2021-11-10 | 2021-11-10 | Cell pipetting gun |
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| CN114107009A CN114107009A (en) | 2022-03-01 |
| CN114107009B true CN114107009B (en) | 2023-08-15 |
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| CN114517182A (en) * | 2022-03-21 | 2022-05-20 | 山东大学 | T cell sorting method for improving tumor killing capability of immune cells |
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