CN114033349B - Seepage-proofing method of in-situ leaching sandstone type uranium deposit based on microbial cementation - Google Patents
Seepage-proofing method of in-situ leaching sandstone type uranium deposit based on microbial cementation Download PDFInfo
- Publication number
- CN114033349B CN114033349B CN202111283747.9A CN202111283747A CN114033349B CN 114033349 B CN114033349 B CN 114033349B CN 202111283747 A CN202111283747 A CN 202111283747A CN 114033349 B CN114033349 B CN 114033349B
- Authority
- CN
- China
- Prior art keywords
- uranium
- situ leaching
- seepage
- sandstone
- cementation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229910052770 Uranium Inorganic materials 0.000 title claims abstract description 120
- JFALSRSLKYAFGM-UHFFFAOYSA-N uranium(0) Chemical compound [U] JFALSRSLKYAFGM-UHFFFAOYSA-N 0.000 title claims abstract description 120
- 238000002386 leaching Methods 0.000 title claims abstract description 117
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 95
- 238000000034 method Methods 0.000 title claims abstract description 49
- 230000000813 microbial effect Effects 0.000 title claims abstract description 34
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 64
- 239000007788 liquid Substances 0.000 claims abstract description 44
- 239000001963 growth medium Substances 0.000 claims abstract description 34
- 238000005065 mining Methods 0.000 claims abstract description 34
- 108060003393 Granulin Proteins 0.000 claims abstract description 33
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 31
- 244000005700 microbiome Species 0.000 claims abstract description 29
- 230000000694 effects Effects 0.000 claims abstract description 28
- 238000001556 precipitation Methods 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 238000012258 culturing Methods 0.000 claims abstract description 9
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 24
- 229910052791 calcium Inorganic materials 0.000 claims description 24
- 239000011575 calcium Substances 0.000 claims description 24
- 238000004519 manufacturing process Methods 0.000 claims description 23
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 22
- 239000004202 carbamide Substances 0.000 claims description 22
- 241000894006 Bacteria Species 0.000 claims description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 18
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims description 13
- 230000001580 bacterial effect Effects 0.000 claims description 13
- 230000035699 permeability Effects 0.000 claims description 13
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims description 12
- 230000012010 growth Effects 0.000 claims description 12
- 230000002265 prevention Effects 0.000 claims description 12
- 238000000605 extraction Methods 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 10
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 9
- 235000010469 Glycine max Nutrition 0.000 claims description 9
- 244000068988 Glycine max Species 0.000 claims description 9
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 9
- 239000002609 medium Substances 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 108010009004 proteose-peptone Proteins 0.000 claims description 9
- 239000011780 sodium chloride Substances 0.000 claims description 9
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 8
- 239000001110 calcium chloride Substances 0.000 claims description 8
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 8
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 claims description 7
- 239000001639 calcium acetate Substances 0.000 claims description 7
- 229960005147 calcium acetate Drugs 0.000 claims description 7
- 235000011092 calcium acetate Nutrition 0.000 claims description 7
- 238000009630 liquid culture Methods 0.000 claims description 7
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 6
- 102000002322 Egg Proteins Human genes 0.000 claims description 6
- 108010000912 Egg Proteins Proteins 0.000 claims description 6
- 235000014103 egg white Nutrition 0.000 claims description 6
- 210000000969 egg white Anatomy 0.000 claims description 6
- 230000003213 activating effect Effects 0.000 claims description 5
- 230000033558 biomineral tissue development Effects 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 4
- 102000008186 Collagen Human genes 0.000 claims description 4
- 108010035532 Collagen Proteins 0.000 claims description 4
- 108010022355 Fibroins Proteins 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- 230000003698 anagen phase Effects 0.000 claims description 4
- 229940098773 bovine serum albumin Drugs 0.000 claims description 4
- 229920001436 collagen Polymers 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 241000193386 Lysinibacillus sphaericus Species 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 230000001939 inductive effect Effects 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 241000894007 species Species 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims 1
- 230000006698 induction Effects 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 24
- 238000011161 development Methods 0.000 abstract description 15
- 239000003673 groundwater Substances 0.000 abstract description 14
- 239000003344 environmental pollutant Substances 0.000 abstract description 8
- 231100000719 pollutant Toxicity 0.000 abstract description 8
- 230000002829 reductive effect Effects 0.000 abstract description 7
- 108010046334 Urease Proteins 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 6
- 230000002411 adverse Effects 0.000 abstract description 4
- 230000002457 bidirectional effect Effects 0.000 abstract description 4
- 239000011435 rock Substances 0.000 abstract description 4
- 238000005728 strengthening Methods 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 239000011148 porous material Substances 0.000 abstract description 2
- 238000012216 screening Methods 0.000 abstract description 2
- 238000004088 simulation Methods 0.000 abstract 1
- 230000018109 developmental process Effects 0.000 description 14
- 239000000243 solution Substances 0.000 description 10
- 230000006872 improvement Effects 0.000 description 9
- 238000011056 performance test Methods 0.000 description 9
- 239000013078 crystal Substances 0.000 description 7
- 239000004576 sand Substances 0.000 description 6
- 241000282414 Homo sapiens Species 0.000 description 5
- 239000003651 drinking water Substances 0.000 description 5
- 235000020188 drinking water Nutrition 0.000 description 5
- 229910001385 heavy metal Inorganic materials 0.000 description 5
- 229910021532 Calcite Inorganic materials 0.000 description 4
- 230000004913 activation Effects 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 238000012512 characterization method Methods 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 230000002093 peripheral effect Effects 0.000 description 3
- 238000005086 pumping Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000004888 barrier function Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000007123 defense Effects 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000002289 effect on microbe Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003758 nuclear fuel Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/28—Dissolving minerals other than hydrocarbons, e.g. by an alkaline or acid leaching agent
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21C—MINING OR QUARRYING
- E21C41/00—Methods of underground or surface mining; Layouts therefor
- E21C41/16—Methods of underground mining; Layouts therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/20—Recycling
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mining & Mineral Resources (AREA)
- Health & Medical Sciences (AREA)
- Geology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Geochemistry & Mineralogy (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Remote Sensing (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Environmental & Geological Engineering (AREA)
- Fluid Mechanics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides an anti-seepage method of an in-situ leaching sandstone uranium deposit based on microbial cementation, which comprises the steps of domesticating an initial strain into a strain with high-efficiency urease activity in an in-situ leaching uranium mining simulation environment, culturing and breeding the strain, and screening an optimal water-soluble organic matrix protein with the effect of strengthening the calcium carbonate precipitation induced by microorganisms; the domesticated strain, the culture medium, the cementing liquid and the optimal water-soluble organic matrix protein are prepared according to a certain proportion, and then are injected into the aquifer of the in-situ leaching sandstone type uranium deposit, and the cementing blocks the pores in the aquifer, so that the anti-seepage effect is achieved. According to the invention, a technology of microorganism-induced calcium carbonate precipitation cementation seepage-proofing water-resisting layer is introduced into the actual environment of the in-situ leaching uranium mining mine, so that the seepage-proofing performance of surrounding rock of the water-bearing layer of the sandstone-type uranium mining mountain is improved, the adverse effect caused by the bidirectional permeation of pollutants and groundwater in the in-situ leaching uranium mining process is reduced, the pollution of groundwater environment is controlled and reduced, and important theoretical guidance and technical support are provided for solving the development bottleneck of the in-situ leaching uranium mining technology.
Description
Technical Field
The invention relates to the technical field of in-situ leaching uranium mining, in particular to an anti-seepage method of in-situ leaching sandstone type uranium ores based on microbial cementation.
Background
With the rapid growth of national economy, the high-speed development of nuclear power industry greatly increases the demand for uranium resources. Uranium is used as an important nuclear fuel, is widely applied to nuclear power plants and navigation, and is also an important national defense strategy resource. Meanwhile, uranium is also an important strategic resource, and is an important guarantee for keeping the nuclear deterrence of China effective for a long time and the sustainable development of nuclear military industry. With the rapid development of nuclear industry and national defense industry in China, a great deal of uranium resources are required to meet the demands of national development strategy, and the production capacity of natural uranium in China must be increased.
Over the last 20 years, the technology of on-site leaching uranium mining has become the main method for natural uranium production at home and abroad, and will still be dominant for a considerable period of time. At present, the natural uranium produced by adopting the on-site leaching technology is close to 50% of the total world yield, and the on-site leaching uranium has the remarkable advantages of low capital investment, low production cost, environmental friendliness and the like. However, leaching solution, U (VI) and heavy metal ions are easy to diffuse to the periphery of an ore-bearing aquifer and an upper aquifer and a lower aquifer in the process of leaching uranium extraction on the spot, so that the drinking water safety of human beings is greatly influenced; and leaching solution diffuses and runs off to the periphery of the mining area, so that the consumption and waste of leaching agent are increased, and more importantly, the peripheral groundwater is adversely affected; conversely, if a large amount of peripheral groundwater is flushed into the seam, the leaching solution is diluted, and the leaching efficiency is reduced. In addition, after the mining area is retired, the underground water still contains SO 4 2- ，NO 3 - ，U(VI)、H + And heavy metal ions and the like, the pollutants threaten downstream groundwater resources through permeation migration, and have great influence on industrial and agricultural production and drinking water safety of human beings, and become bottlenecks for restricting development of uranium leaching technology. Therefore, pollution must be strictly controlled and eliminated from the source, so that the isolation layers around the in-situ leaching uranium mining area and the top and bottom plates have good water stopping effect; the method for controlling the diffusion of leaching solution to the periphery and the flushing of peripheral groundwater into the ore layer is researched, so that the clean, green and efficient development of the on-site leaching uranium mining technology is realized, and the method is a scientific problem which has great practical significance and is solved by the on-site leaching uranium mining engineering technicians and scientific researchers facing urgent needs.
At present, in order to reduce the influence of leaching solution, U (VI) and heavy metal ions on groundwater and surrounding environment, a method for controlling the balance of pumping and injecting liquid is mainly adopted, and although the diffusion of pollutants to groundwater environment can be effectively controlled to a certain extent, the method is influenced by factors such as stratum conditions, pumping and injecting technology, continuous and stable operation capability of a pumping and injecting system and the like, so that the purposes of accurately controlling and thoroughly radically treating the pollutants are difficult to achieve. In recent years, methods for improving the permeation resistance and strength of media have been proposed by filling pores in the media with microbial-induced calcium carbonate precipitation (MICP) bonds; however, the method has the defects of low structural strength of the generated calcium carbonate, toxicity, harm, lack of evaluation and the like of microorganism metabolic end products, and still needs to be studied in depth from the aspects of culture of dominant bacteria, strengthening of organic matters, microorganism cementation mechanism and the like.
In addition, the existing MICP technology is used for repairing ancient architecture and concrete cracks, solidifying heavy metals, modifying rock soil, sealing microorganism and the like, but the problems of high environmental selectivity, complex action mechanism between microorganism and the environment are caused by microorganism growth, and the like, so that whether the MICP technology can reach the research results in the complex environment is still a problem worthy of marshalling and research. Particularly, in the in-situ leaching uranium mining environment, the environment contains leaching solution, U (VI), heavy metal ions and other pollutants, and contains complex organic matters and other impurities, so that the living environment of microorganisms is bad, and the growth, the reproductive capacity and the cementing effect of strains are affected. Therefore, the application of MICP technology in the complex environment of in-situ leaching uranium extraction is still the main research direction and the technical problem to be overcome in the future.
In view of the above, there is a need to design a method for the prevention of seepage of in-situ leaching sandstone-type uranium ores based on microbial cementation to solve the above-mentioned problems.
Disclosure of Invention
The invention aims to provide an impermeable method of in-situ leaching sandstone uranium ores based on microbial cementation. The dominant strain with high-efficiency urease activity is obtained through domestication, and the optimal water-soluble organic matrix protein with the function of strengthening microorganism-induced calcium carbonate precipitation is screened to prepare the microorganism-cemented sandstone-type uranium deposit seepage-proof water-proof layer which is suitable for the in-situ leaching uranium extraction environment, so that the high-efficiency cementing seepage-proof of the in-situ leaching sandstone-type uranium deposit is realized.
In order to achieve the aim of the invention, the invention provides an impermeable method of a ground-immersed sandstone uranium deposit based on microbial cementation, which comprises the following steps:
s1, acclimatization of carbonate mineralized bacteria in-situ leaching uranium mining environment
Activating carbonate mineralized bacteria, and culturing full colonies at constant temperature;
culturing the culture medium with the pH value of 7.3 after sterilization treatment with the full colony to obtain an domesticated strain, carrying out passage according to the inoculation amount of 1% in the growth logarithmic phase, and ending the culture when the growth speed of the domesticated strain is close to that of the original strain; the culture medium is prepared from sandstone-type uranium ore on-site leaching production liquid;
s2, placing the domesticated strain in the step S1 at the same temperature as the in-situ leaching uranium mining environment for expansion culture;
s3, uniformly mixing the bacterial liquid in a certain logarithmic growth phase obtained in the step S2, the culture medium and the optimal water-soluble organic matrix protein, injecting the mixture into an in-situ leaching uranium deposit aquifer with a known permeability coefficient, and then injecting cementing liquid to obtain a microbial cemented sandstone in-situ leaching uranium deposit impermeable water-resisting layer;
and S4, evaluating the impermeability of the impermeable water-resistant layer according to the permeability coefficient of the impermeable water-resistant layer in the step S3, and optimizing the cementing liquid proportion to determine the optimal material proportion in the application environment, so as to finally realize the cementation impermeable of the in-situ-immersed sandstone uranium ore.
As a further improvement of the present invention, in step S3, the optimal water-soluble organic matrix protein is selected by testing the organic matrix protein for enhancement of microorganism-induced calcium carbonate precipitation.
As a further improvement of the invention, the microorganism induces calcium carbonate precipitation to be carried out in a simulated in-situ uranium leaching environment, and the microorganism is the domesticated strain.
As a further improvement of the present invention, in step S1, the medium includes a solid medium and a liquid medium.
As a further improvement of the invention, the liquid culture medium is prepared by dissolving 10-20 g of casein peptone, 2-8 g of soybean peptone, 2-8 g of sodium chloride and 15-25 g of urea in an on-site leaching production liquid.
As a further improvement of the invention, the solid culture medium is prepared by dissolving 10-20 g of casein peptone, 2-8 g of soybean peptone, 2-8 g of sodium chloride, 15-25 g of urea and 15-20 g of agar powder in an on-site leaching production liquid.
As a further improvement of the invention, in the step S3, the dosage ratio of the domesticated strain, the cementing liquid and the optimal water-soluble organic matrix protein is 1 (10-30): 5-15.
As a further improvement of the invention, in step S1, the carbonate mineralizer is bacillus pasteurizer or bacillus sphaericus and other species having the ability to induce carbonate mineralization, the seed inoculum size being 1% of the volume of the culture medium.
As a further improvement of the present invention, the optimal water-soluble organic matrix protein is one of egg white, bovine serum albumin, collagen and fibroin.
As a further improvement of the invention, the cementing liquid comprises urea and a calcium source, wherein the molar concentration of the urea and the calcium source is 0.5-2.5 mol/L, and the concentration and the volume consumption are the same; the calcium source is one of calcium chloride, calcium acetate or calcium nitrate.
The beneficial effects of the invention are as follows:
1. the invention provides an anti-seepage method of an in-situ leaching sandstone uranium deposit based on microbial cementation, which comprises the steps of screening strains with high-efficiency urease activity in an in-situ leaching uranium mining environment, activating strains, growing and breeding, preparing domesticated strains, a culture medium, urea and a calcium source according to a certain proportion, and then injecting the strains into an aqueous layer of the in-situ leaching sandstone uranium deposit. The method ensures that the microorganism-induced calcium carbonate precipitation (MICP) technology is suitable for the complex environment of in-situ leaching uranium extraction, reduces the adverse effect generated by the bidirectional permeation of pollutants and groundwater in the in-situ leaching uranium extraction process, prevents the in-situ leaching uranium from affecting the drinking water safety of human beings, and provides important theoretical guidance and technical support for solving the development bottleneck of the in-situ leaching uranium extraction technology.
2. The invention adopts the in-situ leaching production liquid to prepare the culture medium, carries out environment domestication on mineralized strains, and enables the mineralized strains to adapt to the environment of in-situ leaching uranium extraction; the technology of microorganism-induced calcium carbonate precipitation cementation seepage-proofing water-resisting layer is introduced into the actual environment of the in-situ leaching uranium mine for the first time, so that the seepage-proofing performance of surrounding rock of the in-situ leaching uranium mine ore-bearing water-bearing layer is improved and improved, the groundwater environment pollution is controlled and reduced, the development bottleneck of the in-situ leaching uranium technology is solved, and a new thought is provided for the further development of the in-situ leaching uranium technology.
3. According to the invention, organic matrix proteins are added in a simulated in-situ leaching uranium mining environment to perform microorganism-induced calcium carbonate precipitation, so that the optimal water-soluble organic matrix proteins with the maximum effect enhancing effect on the microorganism-induced calcium carbonate precipitation are obtained; the method is applied to a technology of the calcium carbonate precipitation cementing impermeable water-proof layer induced by microorganisms in the actual leaching mining, so that the structural strength of the generated calcium carbonate is improved, and the impermeable effect of the impermeable water-proof layer of the leaching uranium mine is enhanced.
Drawings
Fig. 1 is a scanning electron microscope characterization result of a microbial cemented sandstone type in-situ leaching uranium mine impermeable water-barrier prepared in example 1 of the present invention.
FIG. 2 is a scanning electron microscope image of a microbial cemented sandstone impermeable water-resistant layer under different calcium source conditions, wherein (a) is calcium chloride, (b) is calcium acetate, and (c) is calcium nitrate.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in detail with reference to the accompanying drawings and specific embodiments.
It should be noted that, in order to avoid obscuring the present invention due to unnecessary details, only structures and/or processing steps closely related to aspects of the present invention are shown in the drawings, and other details not greatly related to the present invention are omitted.
In addition, it should be further noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The seepage prevention method of the in-situ leaching sandstone type uranium deposit based on microbial cementation comprises the following steps of:
s1, acclimatization of carbonate mineralized bacteria in-situ leaching uranium mining environment
(1) Activating the purchased carbonate mineralized bacteria, namely melting the bacteria frozen at-80 ℃ for a long time at normal temperature, inoculating the bacteria liquid on a solid plate by using a sterilized inoculating loop, then placing the plate in a 20 ℃ incubator for culturing, and selecting single bacterial colony for expansion culture after full bacterial colony grows out;
(2) 20mL of the liquid medium was filled into 100mL standard Erlenmeyer flasks and sterilized. The full single colony on the activation plate is picked up and added into a shake flask for inoculation, and the culture is carried out at 20 ℃ and 200 rpm. The culture was terminated when the growth rate of the domesticated bacteria was close to that of the original bacteria by passaging at 1% of the inoculation amount in the log phase of growth.
S2, placing the domesticated strain in the step S1 at the same temperature as the in-situ leaching uranium mining environment for expansion culture;
s3, taking a certain amount of bacterial liquid in the logarithmic growth phase, a culture medium and optimal water-soluble organic matrix protein in the step S2, uniformly mixing, injecting into an in-situ leaching uranium deposit aquifer with a known permeability coefficient, and then injecting cementing liquid to obtain a microbial cemented sandstone in-situ leaching uranium deposit impermeable water-resisting layer;
s4, evaluating the impermeability of the water-resistant layer material according to the permeability coefficient of the water-resistant layer material prepared in the step S3, and optimizing the cementing liquid ratio to determine the optimal material ratio in the application environment.
According to the method, a technology of microorganism-induced calcium carbonate precipitation cementation impermeable water-proof layer is introduced into an actual environment of the in-situ leaching uranium mining mountain for the first time, so that the impermeable performance and the water-stopping effect of surrounding rock of the in-situ leaching uranium mining mountain ore-bearing water-bearing layer are improved and improved, the groundwater environment pollution is controlled and reduced, the development bottleneck of the in-situ leaching uranium mining technology is solved, and a new thought is provided for further development of the in-situ leaching uranium mining technology.
Specifically, in step S3, the optimal water-soluble organic matrix protein is selected by testing the enhancement of the microorganism-induced calcium carbonate precipitation by the organic matrix protein. The microorganism-induced calcium carbonate precipitation is carried out in a simulated uranium leaching environment, and the microorganism is an domesticated strain. The method specifically comprises the following steps:
(1) performing expansion culture on the domesticated strain;
(2) five groups of experiments were set, namely five 100mL standard conical flasks were prepared, 20mL of liquid medium was added to each flask, sterilization was performed, and then 200uL of the logarithmic phase bacterial liquid in the expanded medium was separately aspirated into each conical flask. Wherein, 1-4 groups are added with cementing liquid, 10% of egg white, bovine serum albumin, collagen and fibroin respectively, and 5 groups are control groups, and only cementing liquid and deionized water with the same volume are added. The temperature is set at 20 ℃, the initial pH is set at 7.3, and the reaction time is set at 24 hours;
(3) comparing the calcium carbonate production in the four experimental groups added with the organic matrix protein with the calcium carbonate production in the control group to obtain the optimal organic matrix protein with the maximum calcium carbonate production.
Adding organic matrix protein under simulated in-situ leaching uranium extraction environment to perform microorganism-induced calcium carbonate precipitation to obtain optimal water-soluble organic matrix protein with maximum effect enhancement effect on microorganism-induced calcium carbonate precipitation; and the method is applied to the technology of the microbial induced calcium carbonate precipitation cementing impermeable water-proof layer in the actual leaching mining, so that the structural strength of the generated calcium carbonate is improved, and the impermeable effect of the impermeable water-proof layer in the uranium mine is enhanced.
Specifically, in step S1, the carbonate mineralization bacteria are bacillus pasteurizus or bacillus sphaericus and other strains with functions of inducing carbonate mineralization, and the inoculation amount of the strains is 1% of the volume of the culture medium; the culture medium is prepared from sandstone-type uranium ore on-site leaching production liquid and comprises a solid culture medium and a liquid culture medium. Wherein, the liquid culture medium is prepared by dissolving 10-20 g of casein peptone, 2-8 g of soybean peptone, 2-8 g of sodium chloride and 15-25 g of urea in the in-situ leaching production liquid; the solid culture medium is prepared by dissolving 10-20 g of casein peptone, 2-8 g of soybean peptone, 2-8 g of sodium chloride, 15-25 g of urea and 15-20 g of agar powder in an on-site leaching production liquid.
Specifically, in the step S3, the dosage ratio of the domesticated strain, the cementing liquid and the optimal water-soluble organic matrix protein is 1 (10-30): 5-15; the cementing liquid comprises urea and a calcium source, wherein the calcium source is one of calcium chloride, calcium acetate or calcium nitrate; the molar concentration of urea and calcium source is 0.5-2.5 mol/L, and the concentration and the volume consumption are the same.
In the method, bacterial strains with high-efficiency urease activity are screened out in an in-situ leaching uranium mining environment, bacterial strain activation, growth and reproduction are carried out, domesticated bacterial strains, a culture medium, urea and a calcium source are prepared according to a certain proportion, and then the bacterial strains are injected into an aqueous layer of the in-situ leaching sandstone type uranium deposit. The method ensures that the microorganism-induced calcium carbonate precipitation (MICP) technology is suitable for the complex environment of in-situ leaching uranium mining, reduces the bidirectional permeation of pollutants and groundwater in the in-situ leaching uranium mining process, prevents the in-situ leaching uranium from influencing the drinking water safety of human beings, and provides important theoretical guidance and technical support for solving the development bottleneck of the in-situ leaching uranium mining technology.
Example 1
The seepage prevention method for the in-situ leaching sandstone uranium deposit based on microbial cementation provided by the embodiment comprises the following steps:
s1, placing bacillus pasteurizer (purchased from American type culture Collection, accession number ATCC 11859) in an in-situ uranium leaching environment for domestication, wherein the domestication method of the bacillus pasteurizer comprises the following steps:
(1) activating the purchased carbonate mineralized bacteria, namely melting the bacteria frozen at-80 ℃ for a long time at normal temperature, inoculating the bacteria liquid on a solid plate by using a sterilized inoculating loop, then placing the plate in a 20 ℃ incubator for culturing, and selecting single bacterial colony for expansion culture after full bacterial colony grows out.
(2) 20mL of the liquid medium was filled into 100mL standard Erlenmeyer flasks and sterilized. Selecting full single colonies on an activation plate, adding the single colonies into a shake flask, inoculating, culturing at 20 ℃ and 200rpm, carrying out passage according to 1% of inoculum size in a logarithmic growth phase, and ending the culture when the growth speed of domesticated bacteria is close to that of original bacteria;
wherein, the domestication culture medium of the bacillus pasteurizer adopts a liquid culture medium, each 1000mL of the liquid culture medium is prepared by dissolving 15g of casein peptone, 5g of soybean peptone, 5g of sodium chloride and 20g of urea in an in-situ leaching production liquid, and the in-situ leaching production liquid is used for fixing the volume to 1000mL, the pH value is 7.3, and the sterilization is carried out for 30min;
s2, uniformly mixing the dominant strain domesticated in the step S1 with a culture medium and egg white, culturing at the same temperature as the in-situ leaching uranium extraction environment, injecting into an in-situ leaching uranium ore mountain ore-bearing aquifer, and then injecting cementing liquid (urea+calcium chloride solution) to obtain the microbial cemented sandstone-type in-situ leaching uranium ore aquifer material;
wherein the dosage ratio of the domesticated strain to urea, calcium chloride and egg white is 1:10:10:8, the molar concentration of urea and calcium chloride is 1mol/L, and the volume dosage is the same;
s3, measuring the permeability coefficient of the water-resisting layer material prepared in the step S2, and optimizing the ratio of the bacterial liquid to the cementing liquid to determine the optimal ratio in the current application environment.
FIG. 1 is a scanning electron microscope characterization result of the microorganism-cemented sandstone type in-situ uranium deposit water barrier manufactured in this example, and a large amount of CaCO between sandstone particles can be clearly seen by a Scanning Electron Microscope (SEM) 3 The crystals are filled and gelled, the crystals are irregularly granular and have a phenomenon of crystal overlapping, and the main component of the crystals is calcite crystals. The calcite crystals bond adjacent sand grains together, so that the sand grains are solidified into a whole, the porosity of sand samples is reduced, the permeability of the sand is reduced, the bonding strength among the sand grains is improved, and the shear strength of the sand is greatly improved.
Example 2
Compared with the embodiment 1, the seepage prevention method for the in-situ leaching sandstone uranium ore based on microbial cementation is different in that a solid culture medium is adopted as a domestication culture medium of bacillus pasteurizus, 15g of casein peptone, 5g of soybean peptone, 5g of sodium chloride, 20g of urea and 20g of agar powder are dissolved in an in-situ leaching production liquid per 1000mL of the solid culture medium, and the in-situ leaching production liquid is used for dissolving to 1000mL, and the pH is 7.3.
Examples 3 to 4
The seepage prevention method for the in-situ leaching sandstone uranium ores based on microbial cementation provided in examples 3 to 4 is different from example 1 in that calcium sources are calcium acetate and calcium nitrate respectively, and the rest is the same as example 1, and is not repeated here.
The results of scanning electron microscope characterization analysis of the cemented sandstone type in-situ uranium deposit impermeable water-barrier materials treated by different calcium sources in example 1, example 3 and example 4 are shown in fig. 2, wherein (a), (b) and (c) respectively use calcium chloride, calcium acetate and calcium nitrate as calcium sources. As can be seen from the results, calcite crystals are produced between the sandstone particles to which different calcium sources are added, but the calcium source used in example 1 is calcium chloride, the amount of calcite being the greatest; in addition, aragonite and vaterite are present in the other prepared barrier layers except those prepared with calcium acetate as the calcium source.
Example 5
The seepage-proofing method for the in-situ leaching sandstone uranium ore based on microbial cementation provided in example 5 is different from that in example 1 in that the strain used is not domesticated, and the rest is the same as that in example 1, and the performance test results of example 1 and example 5 are shown in the following table.
Example 6
The method for preventing seepage of a microbial cementation-based in-situ-leaching sandstone uranium ore provided in example 6 is different from example 1 in that a liquid culture medium used in domestication of strains, 15g of casein peptone, 5g of soybean peptone, 5g of sodium chloride and 20g of urea are mixed, dissolved in a mixed solution composed of 50% deionized water and 50% in-situ leaching production solution, and the mixed solution is used for volume fixation to 1000mL, pH is adjusted to 7.3, and sterilization is performed for 30min; the remainder is the same as example 1, and the results of the performance tests of example 1 and example 6 are shown in the following table, and are not repeated here.
Table 1 results of performance tests of example 1 and examples 5-6
As is clear from Table 1, the permeation coefficient of example 5 was significantly higher than that of examples 1 and 6 when the domesticated strain was used, and the permeation coefficient was decreased to a lower extent before and after the cementation than that of examples 1 and 6. It is indicated whether the domestication of the strain can significantly affect the anti-seepage performance of the in-situ-immersed uranium mine water-proof layer, and the domestication of the strain by adopting the in-situ-immersed production liquid to prepare the culture medium in the embodiment 1 has the best anti-seepage performance of the in-situ-immersed uranium mine water-proof layer, so that the anti-seepage performance and the water-stopping effect of the in-situ-immersed uranium mine water-proof layer can be significantly improved.
Examples 7 to 10
Compared with example 1, the seepage prevention method for the in-situ leaching sandstone uranium ore based on microbial cementation provided in examples 7-10 is different in that the organic matrix proteins used in examples 7-9 are bovine serum albumin, collagen and fibroin respectively, no organic matrix protein is added in the experimental process in example 10, the rest is the same as example 1, and the results of the performance tests of examples 1 and examples 7-10 are shown in the following table.
Table 2 results of performance tests of example 1 and examples 7-10
As can be seen from Table 2, the osmotic coefficient of example 10 after the experiment was significantly higher than that of examples 1 and 7-9 without the addition of organic matrix protein, indicating that the organic matrix protein promoted the strain-induced calcium carbonate production. The experimental permeability coefficient of examples 7-9 is slightly larger than that of example 1, and the reduction degree of the experimental permeability coefficient is lower than that of example 1, which shows that the adoption of egg white as the water-soluble organic matrix protein and the cooperation of microorganism to induce calcium carbonate to precipitate the cementing impermeable water-proof layer can obviously improve the impermeability and the water-stopping effect of the impermeable water-proof layer of the in-situ uranium ore.
Examples 11 to 15
The seepage control method for the in-situ leaching sandstone uranium ores based on microbial cementation provided in examples 11 to 15 is different from example 1 in the amount ratio of domesticated strains to urea, calcium source and organic matrix protein, and the rest is the same as example 1, and detailed experimental condition parameters and performance test results are shown in the following table.
TABLE 3 specific experimental condition parameters and performance test results for example 1 and examples 11-15
As shown in Table 3, changing the ratio of the strain to urea, calcium source and organic matrix protein in step S2 has a significant effect on the permeability resistance of the impermeable and water-resistant layer of the in-situ leaching uranium mine, and the impermeable and water-resistant layer of the in-situ leaching uranium mine prepared under the conditions of example 1 has the best permeability resistance. As can be seen from the results of comparative examples 1, 11-14 and 15, the invention can obviously improve the impermeability and water-stopping effect of the impermeable water-resistant layer of the in-situ leaching uranium mine by using the water-soluble organic matrix protein with proper concentration to cooperate with the microorganism to induce the calcium carbonate to precipitate the impermeable water-resistant layer, thereby controlling and reducing groundwater environment pollution and providing a new thought for solving the development of in-situ leaching uranium mining technology.
Examples 16 to 19
The seepage control method for the in-situ leaching sandstone uranium ores based on microbial cementation provided in examples 16 to 19 is different from example 1 in that the molar concentration of the calcium source is changed, and the rest is the same as example 1, and detailed experimental condition parameters and performance test results are shown in the following table.
TABLE 4 specific experimental condition parameters and performance test results for example 1 and examples 16-19
As is clear from the results in Table 4, the activity of Bacillus pasteurizer showed a decreasing trend with increasing concentration of calcium source, indicating that the increase of calcium ion concentration has an effect of inhibiting urease activity, so that the concentration of calcium source in the environment of uranium mining mountain is preferably 0.5 to 2.5mol/L. Meanwhile, as shown in the results of comparative examples 1 and examples 16 to 19, the change of the calcium source concentration has a significant effect on the permeability coefficient of the prepared cement sandstone type in-situ leaching uranium mine impermeable and water-proof layer material, and the impermeable performance of the in-situ leaching uranium mine impermeable and water-proof layer prepared under the condition of example 1 is optimal.
In summary, according to the seepage-proofing method of the in-situ leaching sandstone uranium deposit based on microbial cementation, strains with high-efficiency urease activity are screened out under the in-situ leaching uranium mining environment, strain activation, growth and propagation are carried out, and the optimal water-soluble organic matrix protein with the effect of strengthening microorganism-induced calcium carbonate precipitation is screened out; the domesticated strain, a culture medium, cementing liquid and optimal water-soluble organic matrix protein are prepared according to a certain proportion, and then are injected into an aquifer of the in-situ-immersed sandstone-type uranium deposit, so that the effects of seepage prevention and water isolation are achieved. The invention makes the microorganism induced calcium carbonate precipitation (MICP) technology suitable for the complex environment of in-situ leaching uranium, reduces the adverse effect caused by the bidirectional permeation of pollutants and groundwater in the in-situ leaching uranium, prevents the in-situ leaching uranium from affecting the drinking water safety of human beings, and provides important theoretical guidance and technical support for solving the development bottleneck of the in-situ leaching uranium technology.
The above embodiments are only for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made thereto without departing from the spirit and scope of the technical solution of the present invention.
Claims (8)
1. The seepage-proofing method of the in-situ leaching sandstone uranium deposit based on microbial cementation is characterized by comprising the following steps of:
s1, acclimatization of carbonate mineralized bacteria in-situ leaching uranium mining environment
(1) Activating carbonate mineralized bacteria, and culturing full colonies at constant temperature;
(2) culturing the culture medium with the pH value of 7.3 after sterilization treatment with the full colony to obtain an domesticated strain, carrying out passage according to the inoculation amount of 1% in the growth logarithmic phase, and ending the culture when the growth speed of the domesticated strain is close to that of the original strain; the culture medium is prepared from sandstone-type uranium ore on-site leaching production liquid;
s2, placing the domesticated strain in the step S1 at the same temperature as the in-situ leaching uranium mining environment for expansion culture;
s3, uniformly mixing the bacterial liquid in a certain logarithmic growth phase obtained in the step S2, the culture medium and the optimal water-soluble organic matrix protein, injecting the mixture into an in-situ leaching uranium deposit aquifer with a known permeability coefficient, and then injecting cementing liquid to obtain a microbial cemented sandstone in-situ leaching uranium deposit impermeable water-resisting layer;
the optimal water-soluble organic matrix protein is selected by testing the enhancement effect of the organic matrix protein on the calcium carbonate precipitation induced by microorganisms; the optimal water-soluble organic matrix protein is one of egg white, bovine serum albumin, collagen and fibroin;
and S4, evaluating the impermeability of the impermeable water-resistant layer according to the permeability coefficient of the impermeable water-resistant layer in the step S3, and optimizing the cementing liquid proportion to determine the optimal material proportion in the application environment, so as to finally realize the cementation impermeable of the in-situ-immersed sandstone uranium ore.
2. The method for the prevention of seepage of a sandrock-type uranium deposit impregnated with a matrix based on microbial cementation according to claim 1, characterized in that the microbial induction of calcium carbonate precipitation is carried out in a simulated in-situ uranium extraction environment, the microorganisms being the domesticated strains.
3. The method of claim 1, wherein in step S1, the culture medium comprises a solid culture medium and a liquid culture medium.
4. A method for the prevention of seepage of a sandstone-based uranium deposit by in-situ leaching based on microbial cementation according to claim 3, wherein the liquid medium is prepared from 10 to 20g of casein peptone, 2 to 8g of soybean peptone, 2 to 8g of sodium chloride and 15 to 25g of urea dissolved in the in-situ leaching production fluid.
5. A method for the prevention of seepage of a sandstone-based uranium deposit by in-situ leaching based on microbial cementation according to claim 3, wherein the solid medium is prepared by dissolving 10-20 g of casein peptone, 2-8 g of soybean peptone, 2-8 g of sodium chloride, 15-25 g of urea, 15-20 g of agar powder in the in-situ leaching production liquid.
6. The method for the prevention of seepage of a sandstone-type uranium deposit based on microbial cementation according to claim 1, wherein in step S3, the ratio of the amounts of the domesticated strain, the cementation liquid and the optimal water-soluble organic matrix protein is 1 (10 to 30): 5 to 15.
7. The method for the prevention of seepage of a sandstone-type uranium deposit based on microbial cementation according to claim 1, characterized in that in step S1, the carbonate mineralization bacteria are bacillus pasteurizer or bacillus sphaericus and other species having the function of inducing carbonate mineralization, the inoculum size being 1% of the volume of the culture medium.
8. The method for preventing seepage of a ground-immersed sandstone uranium deposit based on microbial cementation according to claim 6, wherein the cementation solution comprises urea and a calcium source, the molar concentrations of the urea and the calcium source are 0.5-2.5 mol/L, and the concentration and the volume consumption are the same; the calcium source is one of calcium chloride, calcium acetate or calcium nitrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111283747.9A CN114033349B (en) | 2021-11-01 | 2021-11-01 | Seepage-proofing method of in-situ leaching sandstone type uranium deposit based on microbial cementation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111283747.9A CN114033349B (en) | 2021-11-01 | 2021-11-01 | Seepage-proofing method of in-situ leaching sandstone type uranium deposit based on microbial cementation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114033349A CN114033349A (en) | 2022-02-11 |
| CN114033349B true CN114033349B (en) | 2024-01-16 |
Family
ID=80142446
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111283747.9A Active CN114033349B (en) | 2021-11-01 | 2021-11-01 | Seepage-proofing method of in-situ leaching sandstone type uranium deposit based on microbial cementation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114033349B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115709219A (en) * | 2022-11-15 | 2023-02-24 | 长江大学 | Method for preventing petroleum pollutants in soil from migrating |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4270802A (en) * | 1980-01-03 | 1981-06-02 | The United States Of America As Represented Bythe Secretary Of The Interior | Permeability restoration and lowering of uranium leakage from leached ore beds |
| EP0780166A1 (en) * | 1995-12-19 | 1997-06-25 | Canon Kabushiki Kaisha | Method and appararus for remediation of contaminated soil |
| US7294271B1 (en) * | 2005-06-20 | 2007-11-13 | Power Resources, Inc. | Process for restoration of ground water used in in-situ uranium mining |
| CN105297705A (en) * | 2015-10-14 | 2016-02-03 | 内蒙古工业大学 | Biological sandstone preparation method and device based on MICP (Microbial Induced Carbonate Precipitation) technology |
| CN110695052A (en) * | 2019-10-16 | 2020-01-17 | 西南科技大学 | Method for mineralizing and cementing uranium tailing slag by using montmorillonite/carbonate mineralized bacteria |
| CN111997582A (en) * | 2019-05-27 | 2020-11-27 | 惠博普(武汉)生物环保科技有限公司 | Biological treatment technology for hydraulic control sulfur extracted from oil field |
| CN112322519A (en) * | 2020-10-21 | 2021-02-05 | 华中科技大学 | Microbial composite flora for biomineralization and preparation and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008015108A1 (en) * | 2006-08-02 | 2008-02-07 | H.C. Starck Gmbh | Recovery of molybdenum from molybdenum bearing sulfide materials by bioleaching in the presence of iron |
-
2021
- 2021-11-01 CN CN202111283747.9A patent/CN114033349B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4270802A (en) * | 1980-01-03 | 1981-06-02 | The United States Of America As Represented Bythe Secretary Of The Interior | Permeability restoration and lowering of uranium leakage from leached ore beds |
| EP0780166A1 (en) * | 1995-12-19 | 1997-06-25 | Canon Kabushiki Kaisha | Method and appararus for remediation of contaminated soil |
| US7294271B1 (en) * | 2005-06-20 | 2007-11-13 | Power Resources, Inc. | Process for restoration of ground water used in in-situ uranium mining |
| CN105297705A (en) * | 2015-10-14 | 2016-02-03 | 内蒙古工业大学 | Biological sandstone preparation method and device based on MICP (Microbial Induced Carbonate Precipitation) technology |
| CN111997582A (en) * | 2019-05-27 | 2020-11-27 | 惠博普(武汉)生物环保科技有限公司 | Biological treatment technology for hydraulic control sulfur extracted from oil field |
| CN110695052A (en) * | 2019-10-16 | 2020-01-17 | 西南科技大学 | Method for mineralizing and cementing uranium tailing slag by using montmorillonite/carbonate mineralized bacteria |
| CN112322519A (en) * | 2020-10-21 | 2021-02-05 | 华中科技大学 | Microbial composite flora for biomineralization and preparation and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 影响地浸采铀的矿层渗透因素;吉宏斌;刘金辉;殷蓬勃;;世界核地质科学(03);第180-182页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114033349A (en) | 2022-02-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Tang et al. | Factors affecting the performance of microbial-induced carbonate precipitation (MICP) treated soil: a review | |
| Gebru et al. | Bio-cement production using microbially induced calcite precipitation (MICP) method: A review | |
| Cheng et al. | Influence of key environmental conditions on microbially induced cementation for soil stabilization | |
| Xiao et al. | Microbial‐Induced Carbonate Precipitation for Strengthening Soft Clay | |
| Zheng et al. | Low alkali sulpho-aluminate cement encapsulated microbial spores for self-healing cement-based materials | |
| CN110438974B (en) | Microbial solidification kit and method for in-situ solidification of calcareous sand | |
| Sun et al. | Glucose addition improves the bio-remediation efficiency for crack repair | |
| CN103266070A (en) | Bacillus lentus and method for generating by inducing bacillus lentus for reinforcing foundation | |
| Park et al. | Application of Bacillus subtilis 168 as a multifunctional agent for improvement of the durability of cement mortar. | |
| ZHANG et al. | Advances in soil cementation by biologically induced calcium carbonate precipitation | |
| CN114033349B (en) | Seepage-proofing method of in-situ leaching sandstone type uranium deposit based on microbial cementation | |
| CN110813979A (en) | Method for realizing harmless treatment of cyanide tailings by utilizing microbial technology | |
| Sun et al. | Application of bio-remediation with Bacillus megaterium for crack repair at low temperature | |
| Peng et al. | Study of microbially-induced carbonate precipitation for improving coarse-grained salty soil | |
| Fu et al. | Growth and mineralization characteristics of Bacillus subtilis isolated from marine aquaculture wastewater and its application in coastal self-healing concrete | |
| Behzadipour et al. | Effect of microbial-induced calcite precipitation on shear strength of gold mine tailings | |
| Fan et al. | Comparison of bioaugmentation and biostimulation approaches for biocementation in soil column experiments | |
| Sun et al. | Adding aluminum oxide to improve the repairing effect of cracks based on bio-remediation | |
| CN117903991B (en) | Efficient halophilic mineralization strain, and separation screening method and application thereof | |
| CN114216752A (en) | MICP-based indoor weak structural surface reinforcing cementing liquid and test method thereof | |
| Jin et al. | Study on healing technique for weak interlayer and related mechanical properties based on microbially-induced calcium carbonate precipitation | |
| CN117230777A (en) | Method for solidifying granite residual soil | |
| Kadhim et al. | Influences of calcium sources and type of sand on microbial induced carbonate precipitation | |
| CN114985431B (en) | Modified fiber composite material based on microorganism reinforcement process and preparation method thereof | |
| CN115478863A (en) | Granite stratum reinforcing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |