CN103937691B - One plant production β fructosidases aspergillus oryzae strain and its cultural method and application - Google Patents
One plant production β fructosidases aspergillus oryzae strain and its cultural method and application Download PDFInfo
- Publication number
- CN103937691B CN103937691B CN201410177678.7A CN201410177678A CN103937691B CN 103937691 B CN103937691 B CN 103937691B CN 201410177678 A CN201410177678 A CN 201410177678A CN 103937691 B CN103937691 B CN 103937691B
- Authority
- CN
- China
- Prior art keywords
- aspergillus oryzae
- application
- follows
- sucrose
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to one plant of aspergillus oryzae strain for producing β fructosidases and its cultural method and application.One Aspergillus oryzae (Aspergillus oryzae) FS4 bacterial strains, are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 24th, 2014, and deposit number is CGMCC No.9087, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica.The invention further relates to the cultural method of the aspergillus oryzae strain and application.Aspergillus oryzae (Aspergillus oryzae) FS4 strain culturings that present invention screening is obtained are simple, stable hereditary property, combined coefficient is high, and enzyme activity is stable, the fields such as modern biotechnology enzymatic clarification levan type FOSs are can be applied to, are had broad application prospects.
Description
Technical field
The present invention relates to one plant of aspergillus oryzae strain for producing beta -fructosidase enzyme and its cultural method and application, more particularly to one kind
The aspergillus oryzae strain and its cultural method of beta -fructosidase enzyme (EC3.2.1.26) can be produced with synthesizing levan type FOSs
Middle application, belongs to microbial technology field.
Technical background
FOS is that fructosyl is connected to sucrose (GF) point with β (2-1) key (synanthrin type) and β (2-6) key (levan types)
The general designation of fructose oligomer on son.FOS is a kind of suction for having regulation gut flora, breeding Bifidobacterium, promoting calcium
Receive, adjust the novel sweetener of the healthcare functions such as blood fat, anti-caries tooth.The production of current FOS is broadly divided into plant extraction method
And enzyme process.
Plant extraction method is main using witloof as raw material, can only obtain the single of bonding FOS of β (2-1) key, far not
The demand developed to its different physiological roles can be met.Enzymatic conversion method sucrose, can produce a variety of of bonding FOSs, wherein
The more conventional synanthrin type FOS of levan type FOSs has higher physiologically active, by more and more concerns.Rice
Aspergillus is the engineering bacteria commonly used in fermentation industry, from some beta -fructosidase enzymes of aspergillus oryzae, with synthesis of oligonucleotides fructose
Function, available for industrial mass production.
Existing known aspergillus oryzae source beta -fructosidase enzyme, the only report of synanthrin type FOS synthesis, therefore find new
The beta -fructosidase enzyme that can produce levan type FOSs aspergillus oryzae strain, have important to enzymatic clarification FOS
Meaning.
The content of the invention
The aspergillus oryzae strain that the present invention is directed to production FOS in existing microbial enzyme method commercial Application is limited, particularly lacks
There is provided a kind of stability for producing levan type FOSs of new separation is good for the aspergillus oryzae of weary generation levan type FOSs
Beta -fructosidase enzyme aspergillus oryzae strain, this bacterium produce beta -fructosidase enzyme can be with the production of efficient catalytic levan type FOSs
It is raw.
Summary of the invention
The method is characterized in that providing a kind of aspergillus oryzae (Aspergillus oryzae) FS4 bacterial strains for producing beta -fructosidase enzyme
And its feature, cultural method and its method applied to the levan types FOS synthesis using sucrose as substrate.Examined through document
Rope, is applied to the synthesis by substrate levan types FOS of sucrose without the beta -fructosidase enzyme that aspergillus oryzae is originated both at home and abroad at present
Report.
Detailed description of the invention
One Aspergillus oryzae (Aspergillus oryzae) FS4 bacterial strains, are preserved in China Microbiological bacterium on April 24th, 2014
Preservation administration committee common micro-organisms center is planted, deposit number is CGMCC No.9087, address：The Chaoyang District, Beijing City North Star
No. 3 Institute of Microorganism, Academia Sinica of institute of West Road 1.
Aspergillus oryzae (Aspergillus oryzae) the FS4 bacterial strains grow rapidly on potato culture, 2~3 days
Bacterium colony is sprawled, and produces a large amount of mycelium, white, part yellow at the beginning of bacterium colony is changed into green at 7 days or so, with prolonging for incubation time
The long brown that fades to is to crineous, and substrate mycelium produces water-soluble brown pigment.Conidial head is radial, conidiophore top
Capsule is subsphaeroidal or flask shape, overlying bilayer stigma.It is spherical or near ball after ocean pyriform or oblong when conidium is young, maturation
Shape, rough surface has fold.The 18S rDNA of the bacterial strain are EU680477 in GenBank accession number, with an Aspergillus oryzae
18S rDNA sequences (GenBank No.HM064501) homology is 99%.
The cultural method of above-mentioned aspergillus oryzae (Aspergillus oryzae) FS4 bacterial strains, step is as follows：
Picking aspergillus oryzae FS4 inoculations to slant medium is activated 48~60 hours, and activation temperature is 25~30 DEG C, is taken
Aspergillus oryzae FS4 after activation is inoculated in seed culture fluid, is cultivated 48~60 hours, and cultivation temperature is 25~30 DEG C, is produced；
The slant medium component is as follows：Potato 200g/L, glucose 20g/L, agar 15g/L；
The seed culture fluid component is as follows, is mass percent：Sucrose 0.5~4%, dusty yeast 1~4%, carboxylic first
Base sodium cellulosate (CMC) 0.01~0.7%, excess water.
According to currently preferred, the seed culture fluid component is as follows, is mass percent：Sucrose 2%, dusty yeast
3.5%, sodium carboxymethylcellulose (CMC) 0.5%, excess water.
According to currently preferred, the cultivation temperature is 28 DEG C.
Applications of above-mentioned aspergillus oryzae (Aspergillus oryzae) FS4 in production levan type FOSs.
Above-mentioned application, step is as follows：
(1) learn from else's experience the aspergillus oryzae FS4 after above-mentioned culture, transfers and is fermented in producing enzyme by the inoculum concentration of percent by volume 1~5%
In nutrient solution, shaking table culture 48~72 hours, cultivation temperature is 25~30 DEG C, and shaking speed is 150 revs/min, and zymotic fluid is made；
The producing enzyme fermentation culture component is as follows, is weight percentage：Sucrose 0.5~4%, dusty yeast 1~4%,
Sodium carboxymethylcellulose (CMC) 0.01~0.7%, excess water；
(2) zymotic fluid made from step (1), through separation of solid and liquid, is taken into precipitation, mycelium is made；
(3) by mycelium made from step (2) after crushing, by mass volume ratio 1：(1~10) is suspended in reaction buffering
In liquid, unit g/mL, 50~100r/min stir 1~3min, and crude enzyme liquid is made；
(4) by crude enzyme liquid made from step (3) and mass concentration for 20~70% sucrose solution by volume 1:(1~
4) mix, under conditions of temperature is 30~60 DEG C, react 10~400 minutes, be made and contain the molten of levan type FOSs
Liquid.
According to currently preferred, the shaking table culture time in the step (1) is 48 hours.
According to currently preferred, the producing enzyme fermentation culture component in the step (1) is as follows：Sucrose 2%, dusty yeast
3.5%, sodium carboxymethylcellulose (CMC) 0.5%, excess water.
According to currently preferred, the separation of solid and liquid in the step (2) is suction filtration or centrifugation.
According to currently preferred, being broken in the step (3) is broken with liquid nitrogen grinding.
According to currently preferred, reaction buffer is pH7.0, concentration 50mM potassium phosphate buffering in the step (4)
Liquid；Sucrose solution mass concentration is 60% in the step (4).
According to currently preferred, reaction temperature is 45 DEG C in the step (5), and the reaction time is 300 minutes.
Above-mentioned slant medium, seed culture fluid and producing enzyme fermentation culture pH need not be adjusted, using preceding high all at 115 DEG C
The lower sterilizing of temperature 30 minutes.
Beneficial effect
Aspergillus oryzae (Aspergillus oryzae) FS4 cultures that present invention screening is obtained are simple, and stable hereditary property is closed
Into efficiency high, enzyme activity is stable, can be applied to the fields such as modern biotechnology enzymatic clarification levan type FOSs, has
Wide application prospect.
Brief description of the drawings
Fig. 1 is the beta -fructosidase enzyme synthesis levan type FOSs that aspergillus oryzae (Aspergillus oryzae) FS4 is produced
The TLC separation figures of sample；
Fig. 2 is that the beta -fructosidase enzyme synthesis levan types that aspergillus oryzae FS4 (Aspergillus oryzae FS4) is produced are oligomeric
The nuclear magnetic resonance heteronuclear Multiple-Quantum Coherences spectrogram of fructose trisaccharide sample.
Embodiment
Technical scheme is further elaborated with reference to embodiment, but institute's protection domain of the present invention is not only limited
In this.
Embodiment 1
One Aspergillus oryzae (Aspergillus oryzae) FS4 bacterial strains, are preserved in China Microbiological bacterium on April 24th, 2014
Preservation administration committee common micro-organisms center is planted, deposit number is CGMCC No.9087, address：The Chaoyang District, Beijing City North Star
No. 3 Institute of Microorganism, Academia Sinica of institute of West Road 1.
(1) Morphological observation
It is connected to oese picking aspergillus oryzae (Aspergillus oryzae) FS4 on potato culture flat board, 28
DEG C culture.Grown rapidly on potato culture, bacterium colony is sprawled within 2~3 days, produce white, part Huang at the beginning of a large amount of mycelium, bacterium colony
Color, is changed into green at 7 days or so, fade to brown to crineous with the extension of incubation time, substrate mycelium produces water-soluble
Brown pigment.Conidial head is radial, and conidiophore top capsule is subsphaeroidal or flask shape, overlying bilayer stigma.Conidium
It is spherical or subsphaeroidal after ocean pyriform or oblong when young, maturation, rough surface has fold.
Above-mentioned potato culture component is as follows：Potato 200g/L, glucose 20g/L, agar 15g/L.Potato goes
Skin, is cut into block and boils 30 minutes, juice, then sugaring and agar are then taken with filtered through gauze, water is supplied after dissolving to 1L, 115 DEG C go out
Bacterium 30 minutes.
(2) 18S rDNA Sequencing and Characterizations：
Aspergillus oryzae (Aspergillus oryzae) FS4 genomes are extracted with genome extracts kit (BioFlux)
DNA, using genomic DNA as template, using general sense primer NS1 (the 5 '-GTAGTCATATGCTTGTCTC- of fungi 18S rDNA
3 ') and anti-sense primer FS2 (5 '-TAGGNATTCCTCGTTGAAGA-3 ') PCR amplification 18S rDNA sequences.
The μ L of PCR reaction systems 50：10 × PCR buffer5 μ L, 2.5mmol/L dNTP mixtures 4 μ L, 20 μm of ol/L draw
Each 2 μ L of thing, template 2 μ L (10ng), the μ L of LATaq enzymes 1, the μ L of sterilized water 34.PCR amplification conditions：94 DEG C of pre-degenerations 5 minutes, 94 DEG C
Denaturation 30 seconds, 50 DEG C are annealed 30 seconds, and 72 DEG C extend 1.5 minutes, 30 circulations；72 DEG C extend 5 minutes.The amplified production obtained
Sequencing is carried out by Shanghai Sheng Gong bioengineering Co., Ltd, size is 1563 bases, and the sequence is carried out in NCBI websites
BLAST nucleotides compares analysis, and 18S rDNA sequences (GenBank No.HM064501) homology of aspergillus oryzae is 99%..
Aspergillus oryzae (Aspergillus oryzae) FS418S rDNA GenBank accession number is EU680477.
Embodiment 2
The cultural method of aspergillus oryzae (Aspergillus oryzae) FS4 bacterial strains, step is as follows：
Aspergillus oryzae (Aspergillus oryzae) FS4 inoculations are taken to fresh slant medium, in 28 DEG C of condition
Lower activation 48 hours, takes aspergillus oryzae (Aspergillus oryzae) FS4 after activation to be inoculated in 100mL seed culture fluids, trains
Support 48 hours, aspergillus oryzae seed liquor is made；
The slant medium component is as follows：Potato 200g/L, glucose 20g/L, agar 15g/L.115 DEG C sterilize 30 points
Clock.
The seed culture fluid component is as follows, is weight percentage：Sucrose 2%, dusty yeast 3.5%, carboxymethyl cellulose
Sodium (CMC) 0.5%, excess water.115 DEG C sterilize 30 minutes.
Is come into by row 50 and is passed on for aspergillus oryzae (Aspergillus oryzae) FS4 bacterial strains, light microscope and electron microscopic examination,
Bacterial strain through passage is consistent with original strain form.The crude enzyme liquid synthesis levan type FOSs of bacterial strain after the passage of 50 generations
Yield is consistent with original strain.Stability experiment shows that the bacterial strain has very high stability, and number does not have for rear enzymatic productivity
Reduction.
Embodiment 3
The beta -fructosidase enzyme produced using aspergillus oryzae FS4 bacterial strains synthesizes levan type FOSs, comprises the following steps that：
(1) learn from else's experience the aspergillus oryzae FS4 after above-mentioned culture, transfers and is sent out in 500mL producing enzymes by the inoculum concentration of percent by volume 1%
In ferment nutrient solution, shaking table culture 48 hours, cultivation temperature is 28 DEG C, and shaking speed is 150 revs/min, and zymotic fluid is made；
The producing enzyme fermentation culture component is as follows, is weight percentage：Sucrose 2%, dusty yeast 3.5%, carboxymethyl
Sodium cellulosate (CMC) 0.5%, excess water；
(2) by zymotic fluid made from step (1) after suction filtration, mycelium is made；
(3) mycelium made from step (2) is ground through liquid nitrogen after crushing, by mass volume ratio 1：5 are suspended in
In pH7.0, concentration 50mM kaliumphosphate buffer, crude enzyme liquid is made in unit g/mL, 50r/min stirring 1min；
(4) the μ L of crude enzyme liquid 25 made from step (3) are mixed with mass concentration for the 60% μ L of sucrose solution 100, quality
The sucrose solution that concentration is 60% is to be prepared with pH7.0, concentration 50mM kaliumphosphate buffer, in 45 DEG C of shaking bath reactions 5
After hour, the solution containing levan type FOSs is made.
Sampling carries out TLC analyses, as a result as shown in Figure 1.
Above-mentioned TLC analysis device therefors and condition：Reaction product point sample is in TLC thin plates, in developing agent (n-butanol:Ethanol:
Water=5:3:2) exhibition layer in, spray painting developer (3, the 5- orcins of 20% sulfuric acid solution+0.5%) toasts 3 in 120 DEG C
~5 minutes, sugared spot development.
Above-mentioned product quantify, purified and nuclear magnetic resonance identification chemical constitution.Wherein, the nuclear magnetic resonance of ketose
Qualification result is as shown in Figure 2.
Above-mentioned nuclear magnetic resonance spectroscopy device therefor and condition：The reaction product of purifying is dissolved in deuterated water, uses Brooker
DRX600MHz nuclear magnetic resonance chemical analysers detect that the heteronuclear Multiple-Quantum Coherences of product compose (heteronuclear in 26 DEG C
Multiple-bond correlation (HMBC) spectra), the chemical constitution for determining product is the levan types of β (2-6) key
FOS.
Through quantitative experiment, the content that FOS accounts for total reducing sugar in this reaction system is 56%.
Claims (9)
1. aspergillus oryzae（Aspergillus oryzae）Applications of the FS4 in production levan type FOSs；
The aspergillus oryzae（Aspergillus oryzae）FS4 bacterial strains, are preserved in Chinese microorganism strain guarantor on April 24th, 2014
Administration committee's common micro-organisms center is hidden, deposit number is CGMCC No. 9087, address：BeiChen West Road, Chaoyang District, BeiJing City
No. 3 Institute of Microorganism, Academia Sinica of No. 1 institute.
2. application as claimed in claim 1, it is characterised in that step is as follows：
（1）Learn from else's experience the aspergillus oryzae FS4 after above-mentioned culture, is transferred by the inoculum concentration of percent by volume 1~5% in producing enzyme fermented and cultured
In liquid, shaking table culture 48~72 hours, cultivation temperature is 25~30 DEG C, and shaking speed is 150 revs/min, and zymotic fluid is made；
The producing enzyme fermentation culture component is as follows, is weight percentage：Sucrose 0.5~4%, dusty yeast 1~4%, carboxymethyl
Sodium cellulosate 0~0.7%, excess water；
（2）By step（1）Obtained zymotic fluid takes precipitation through separation of solid and liquid, and mycelium is made；
（3）By step（2）Obtained mycelium after crushing, by mass volume ratio 1：（1~10）It is suspended in reaction buffer,
Unit g/mL, 50~100r/min stir 1~3 min, and crude enzyme liquid is made；
（4）By step（3）Obtained crude enzyme liquid and mass concentration for 20~70% sucrose solution by volume 1:（1~4）It is mixed
Close, under conditions of temperature is 30~60 DEG C, react 10~400 minutes, the solution containing levan type FOSs is made.
3. application as claimed in claim 2, it is characterised in that the step（1）Aspergillus oryzae FS4 after middle culture, culture step
It is rapid as follows：
Picking aspergillus oryzae FS4 inoculations to slant medium is activated 48~60 hours, and activation temperature is 25~30 DEG C, takes activation
Aspergillus oryzae FS4 afterwards is inoculated in seed culture fluid, is cultivated 48~60 hours, and cultivation temperature is 25~30 DEG C, is produced；
The slant medium component is as follows：Potato 200g/L, glucose 20g/L, the g/L of agar 15；
The seed culture fluid component is as follows, is mass percent：Sucrose 0.5~4%, dusty yeast 1~4%, carboxymethyl cellulose
Plain sodium 0~0.7%, excess water.
4. application as claimed in claim 3, it is characterised in that the seed culture fluid component is as follows, is mass percent：
Sucrose 2%, dusty yeast 3.5%, sodium carboxymethylcellulose 0.5%, excess water.
5. application as claimed in claim 2, it is characterised in that the step（1）In producing enzyme fermentation culture component it is as follows：
Sucrose 2%, dusty yeast 3.5%, sodium carboxymethylcellulose 0.5%, excess water.
6. application as claimed in claim 2, it is characterised in that the step（2）In separation of solid and liquid be suction filtration or centrifugation.
7. application as claimed in claim 2, it is characterised in that the step（3）In be broken for it is broken with liquid nitrogen grinding.
8. application as claimed in claim 2, it is characterised in that the step（4）Middle reaction buffer is pH7.0, concentration
50mM kaliumphosphate buffer；The step（4）Middle sucrose solution mass concentration is 60%.
9. application as claimed in claim 2, it is characterised in that the step（5）Middle reaction temperature is 45 DEG C, and the reaction time is
300 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410177678.7A CN103937691B (en) | 2014-04-29 | 2014-04-29 | One plant production β fructosidases aspergillus oryzae strain and its cultural method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410177678.7A CN103937691B (en) | 2014-04-29 | 2014-04-29 | One plant production β fructosidases aspergillus oryzae strain and its cultural method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103937691A CN103937691A (en) | 2014-07-23 |
| CN103937691B true CN103937691B (en) | 2017-10-17 |
Family
ID=51185570
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410177678.7A Active CN103937691B (en) | 2014-04-29 | 2014-04-29 | One plant production β fructosidases aspergillus oryzae strain and its cultural method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103937691B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105483027A (en) * | 2016-01-04 | 2016-04-13 | 山东星光生物科技有限公司 | Aspergillus tubingensis and method for producing fructooligosaccharides through whole-cell catalysis of aspergillus tubingensis |
| CN110172407A (en) * | 2018-12-11 | 2019-08-27 | 青岛蔚蓝生物集团有限公司 | One plant of aspergillus oryzae for producing transfructosylase and its application |
| CN109439552B (en) * | 2018-12-26 | 2020-01-21 | 山东百龙创园生物科技股份有限公司 | Aspergillus oryzae BLCY-006 and application thereof in preparation of galactooligosaccharides |
| CN110564626B (en) * | 2019-09-17 | 2021-12-07 | 山东百龙创园生物科技股份有限公司 | Aspergillus oryzae strain, culture method thereof and method for preparing kestose |
| CN111487332A (en) * | 2019-11-08 | 2020-08-04 | 天津科技大学 | Evaluation method and application of prebiotics effect of fructo-oligosaccharide with single polymerization degree |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533605A (en) * | 2012-01-16 | 2012-07-04 | 江南大学 | Strain capable of producing levansucrase and method for producing levan by using levansucrase |
| CN103555690A (en) * | 2013-10-28 | 2014-02-05 | 光明乳业股份有限公司 | Novel fructosidase as well as encoding gene and applications thereof |
-
2014
- 2014-04-29 CN CN201410177678.7A patent/CN103937691B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533605A (en) * | 2012-01-16 | 2012-07-04 | 江南大学 | Strain capable of producing levansucrase and method for producing levan by using levansucrase |
| CN103555690A (en) * | 2013-10-28 | 2014-02-05 | 光明乳业股份有限公司 | Novel fructosidase as well as encoding gene and applications thereof |
Non-Patent Citations (3)
| Title |
|---|
| 产β-呋喃果糖苷酶菌株的筛选及发酵条件初探;赵秀红 等;《食品工业科技》;20061231;第27卷(第6期);第90-92页，参见摘要、第1.1-1.3、2.1节 * |
| 米曲霉β -呋喃果糖苷酶基因克隆及生物信息学分析;唐江涛 等;《食品科学》;20081231;第29卷(第6期);第208-211页 * |
| 米曲霉中提取的β-D-呋喃果糖苷酶的浓缩与纯化;马莺 等;《食品科技》;20011231;第47-55页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103937691A (en) | 2014-07-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102321563B (en) | Amycolatopsis sp. and method for preparing vanillin through whole-cell transformation of Amycolatopsis sp. | |
| US11279961B2 (en) | Aspergillus oryzae BLCY-006 strain and application thereof in preparation of galactooligosaccharide | |
| CN103937691B (en) | One plant production β fructosidases aspergillus oryzae strain and its cultural method and application | |
| CN115873754A (en) | Enteromorpha clotrimaca RS804 and application thereof | |
| CN101550401B (en) | Method for screening yellow wine yeast of low-yield urea | |
| CN102061320B (en) | Preparation method of 11 alpha,17 alpha-dyhydroxyl-androst-4-ene-3,20-dione | |
| CN105695340B (en) | Aspergillus oryzae and application thereof | |
| EU et al. | Screening, isolation and characterization of amylase producing bacteria and optimization for production of amylase | |
| CN114231458B (en) | Compound microbial agent for improving sugar-acid ratio of melons and fruits as well as preparation method and application of compound microbial agent | |
| CN108330082A (en) | One plant of Lactobacillus paracasei and its application | |
| CN101914451B (en) | Alternaria radicina bacterial strain for producing alpha-L-rhamnoside enzyme and cultivating method as well as application thereof | |
| EP3845658B1 (en) | Method for preparing vanillin by fermentation with eugenol as substrate | |
| CN102533607B (en) | Strain capable of producing beta-galactosidase and method for producing galactooligosaccharides by using beta-galactosidase | |
| CN114015607A (en) | Bacillus amyloliquefaciens for high yield of 5-methyltetrahydrofolic acid and application thereof | |
| CN101250484B (en) | Expansion penicillium strain as well as culture method and use thereof | |
| JP2003325166A (en) | METHOD FOR PRODUCING HIGHLY beta-GALACTOSIDASE-PRODUCING MUTANT MICROORGANISM AND MUTANT MICROORGANISM PRODUCED BY THE METHOD AND UTILIZATION THEREOF | |
| CN109456898A (en) | A kind of the fermentation preparation and its application of chaetomium globosum dextranase | |
| JP2004344084A (en) | Alcohol fermentative yeast | |
| KR102084980B1 (en) | Aspergillus oryzae GBE174 and method for preparing fermentated extract of ginseng berry using the same | |
| CN106754829A (en) | A kind of method of utilization bacillus HS17 fermenting and producing chitosan enzymes and its application | |
| CN114369558B (en) | Serratia marcescens and application thereof in naringinase production | |
| CN114958630B (en) | Pichia pastoris strain capable of producing glucuronic acid and application thereof | |
| CN104498542A (en) | Method for preparing L-lactic acid employing continuous method in fermentation manner | |
| CN117384799B (en) | Method for producing beta-glucosidase by using Lu Gesi bacillus A78.1 and microbial inoculum and application | |
| CN118064334B (en) | Paenibacillus polymyxa T9 and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |