CN103876145A - Probiotics micro-ecological tablet and preparation method thereof - Google Patents

Abstract

A probiotic microecological tablet and a preparation method thereof of the present invention belong to the technical field of health food and processing. The probiotic microecological tablet contains 90% to 98% probiotic powder, 1% to 9% fructooligosaccharide and 0.1% to 1% magnesium stearate by mass. The preparation is to activate the bifidobacteria and prepare the kefir starter; then, the two are mixed and fermented, concentrated and dried at low temperature, and mixed and pressed into tablets. Mixed fermentation is to inoculate the mixed strains of probiotics, and ferment for 16-24 hours at 28-36° C., and the ratio of kefir starter to bifidobacteria in the mixed strains is 1-3:1. The present invention carries out high-density mixed fermentation of kefir starter and bifidobacteria, with diverse strains and stable storage, and the number of live bacteria in the tablet reaches more than 2 billion cfu/g respectively; it can not only improve digestibility, but also enhance It improves the colonization ability of probiotics in the intestinal tract; it has the characteristics of high nutritional value and unique flavor.

Description

A kind of probiotic microecological tablet and preparation method thereof

technical field

The invention relates to a probiotic microecological tablet and a preparation method thereof, belonging to the technical field of health food and processing.

Background technique

As a kind of probiotic microecological preparation, probiotic microecological tablets have the functions of adjusting intestinal flora, improving the microecological balance of the human body, and inhibiting the growth and reproduction of harmful pathogenic microorganisms in the human body. The active ingredients in the probiotic microecological tablet are live probiotics. Currently, common probiotics include lactic acid bacteria, bifidobacteria, Lactobacillus bulgaricus, and Streptococcus thermophilus. At present, the commercially available probiotic microecological preparations are mainly solid granules or capsules, and most of the probiotic powders contained in them are processed from a single strain of bacteria powder and auxiliary materials after compounding. , the vitality of the bacteria decreased, which was not enough to achieve the expected health care effect.

In the present invention, kefir starter and bifidobacteria are cultured at high density, mixed and fermented, concentrated and dried at low temperature, and then pressed into tablets to make probiotic micro-ecological tablets, which avoids multiple processing of bacterial powder and improves the yield of probiotics. Live bacteria content. Secondly, most of the currently commercially available probiotic microecological preparations are compounded by a single strain of bacteria. It enriches the types of probiotics and improves the health effect of the product.

Contents of the invention

The purpose of the present invention is to provide a probiotic microecological tablet and its preparation method. The product contains a variety of active probiotics, which can adjust the balance of intestinal flora, promote digestion and absorption, enhance the body's immunity, and treat diarrhea and constipation. .

The present invention is achieved by the following technical means:

The invention relates to a probiotic microecological tablet, which is composed of 90%-98% of probiotic powder, 1%-9% of fructooligosaccharide and 0.1%-1% of magnesium stearate.

In the above tablet, the probiotics are composed of composite bacteria and bifidobacteria in the kefir starter. Composite strains in kefir starter by mass: bifidobacteria = 1 ~ 3: 1.

Probiotic bacteria powder is made from compound bacteria (lactic acid bacteria, yeast, etc.) and bifidobacteria in kefir starter cultured at high density, mixed fermentation, concentrated and dried at low temperature.

In the above tablet, the bifidobacterium is composed of 1 to 3 types of Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium breve and Bifidobacterium adolescent. When 2 to 3 kinds of bifidobacteria are contained, the mass ratio of various bifidobacteria can be arbitrary, and the specific ratio depends on the mixing ratio of various activated bifidobacteria used in the preparation.

In the above tablet, the concentration of viable bacteria is greater than 6 billion cfu/g.

A method for preparing probiotic microecological tablets, firstly using MRS lactose medium to activate bifidobacteria, using skim milk to inoculate kefir grains to prepare kefir starter; it is characterized in that, after mixed fermentation, low temperature Concentrated and dried, mixed and pressed into tablets;

The mixed fermentation is to prepare the medium according to the following process. The components of the medium by weight percentage include concentrated whey protein 2% to 10%, glucose 1% to 10%, and yeast powder 0.5% to 1%. , calcium carbonate 0.4%~1%, magnesium sulfate 0.01%~0.06%, manganese sulfate 0.001%~0.01%, whey powder 1%~10%, inulin 0.5%~3%, ginseng polysaccharide 0.04%~1%, Soybean oligosaccharides 0.02% ~ 1%, the balance is distilled water; dissolve each component in distilled water, adjust the pH to 6.5 ~ 7.5, keep it at 90 ~ 115 ° C for 10 ~ 30 minutes to sterilize; cool to 20 ~ 30 ° C Inoculate the mixed strains of kefir starter and bifidobacteria according to 3% to 10% of the medium volume, and ferment for 16 to 24 hours at 28 to 36°C to obtain a mixed fermentation broth of probiotics. The inoculation ratio of Phil starter and Bifidobacteria is Kefir starter: Bifidobacteria=1～3:1;

The low-temperature concentrated drying is to stir and centrifuge the probiotic mixed fermentation liquid for 15-30 minutes under the condition of 3000-6000 r/min, and collect the concentrated bacteria; Vacuum freeze-drying or low-temperature spray-drying under the condition that the inlet air temperature is 80-120°C and the outlet air temperature is 50-70°C, to obtain the probiotic bacteria powder;

The mixed material pressing is to mix 90%-98% probiotic powder, 1%-9% fructo-oligosaccharide, and 0.1%-1% magnesium stearate evenly according to the mass percentage, and press the tablet to make probiotics Microbial tablet.

The final product is a compound probiotic microecological tablet with a unit viable count greater than 6 billion cfu/g.

The activation of the bifidobacteria can be carried out according to the prior art, or can be carried out according to the following process. The frozen bifidobacteria powder is inoculated on the MRS lactose medium, and after being cultivated at 37°C for 24 hours, the microscopic examination conforms to The standard form of bifidobacteria is in a good growth state, and then inoculated with 3% to 6% of the inoculum in MRS lactose medium for activation, and cultured at 37°C for 18 to 24 hours.

The above-mentioned Bifidobacterium powder can be Bifidobacterium infantis powder, Bifidobacterium longum powder, Bifidobacterium breve powder or/and Bifidobacterium adolescentis powder; Mix to obtain activated bifidobacteria for mixed fermentation with kefir starter.

The preparation of the kefir starter can be carried out according to the prior art, and can also be carried out according to the following process, using skim milk powder to make a 10% skim milk solution, after sterilizing at 90 to 115 ° C for 10 to 30 minutes, Cool to 20-30°C, inoculate kefir grains according to 0.1%-0.5% of the volume of skim milk, and ferment at 23-28°C for 14-18 hours.

In the low-temperature concentration and drying process, the protective agent is based on the weight of the probiotic mixed fermentation broth, consisting of 10% to 20% of skim milk powder, 1% to 10% of trehalose, and 1% to 10% of sodium glutamate. %, glycerin 1% to 5%.

In the mixing and tableting process, the tableting can be carried out by using a tableting machine under the condition of a pressure of 4000-10000N. It can be pressed into tablets with a diameter of 6-12mm, a tablet weight of 0.3-0.5g, and a hardness of 8-10kg/ ^cm2 .

The beneficial effects of the present invention are:

1) In the present invention, kefir starter and bifidobacteria are mixed and fermented at high density, with various strains and stable storage, and the number of viable bacteria in the tablet can reach more than 2 billion cfu/g respectively;

2) The mixed fermentation of kefir starter and bifidobacteria can not only improve the digestion ability, but also enhance the colonization ability of probiotics in the intestine, enrich the intestinal flora, improve the micro-ecological balance of the intestinal tract, It has a good effect on maintaining intestinal health;

3) The present invention contains abundant probiotics and their active metabolites, and adds fructooligosaccharides, which has the characteristics of high nutritional value and unique flavor.

Detailed ways

The following examples are to further illustrate the present invention, rather than limit the present invention.

Example 1:

1) Preparation of probiotic starter

① Preparation of bifidobacteria

Inoculate the frozen Bifidobacterium adolescent powder into MRS lactose medium, culture at 37°C for 24 hours, and the microscopic examination conforms to the standard form of bifidobacteria, and the growth state is good. Inoculate 3% of the inoculum into MRS lactose medium For activation, culture at 37°C for 24 hours and save for later use.

② Preparation of Kefir starter

Use skim milk powder to make 10% skim milk solution, after sterilizing at 115°C for 30 minutes, cool to 25°C, inoculate kefir grains with 0.1% of the volume of skim milk, ferment at 23°C for 18 hours and store for later use .

2) Mixed fermentation

Take concentrated whey protein 10g, glucose 6.7g, yeast powder 3.8g, calcium carbonate 3.4g, magnesium sulfate 0.2g, manganese sulfate 0.015g, whey powder 14g, inulin 2.5g, ginseng polysaccharide 0.5g, soybean oligosaccharide 1g, add distilled water to a total mass of 500g. After fully dissolving, adjust the pH of the medium to 7.0, and the sterilization condition is to keep warm at 115°C for 30 minutes, and cool to 30°C for later use.

Inoculate the cooled high-density medium with 3% inoculum, kefir starter: Bifidobacterium adolescent = 2:1, 28°C, and ferment for 24 hours.

3) Preparation of bacteria powder

Stir the fermented broth evenly, and centrifuge at 6000 rpm for 15 minutes at 20°C to collect concentrated bacteria; add 10% skim milk powder and 5% trehalose to the collected bacteria , 1% sodium glutamate, 3% glycerin, mix evenly, adjust the dry matter concentration to 30-40% with sterile water, and carry out under the condition that the air inlet temperature is 80-120°C and the outlet air temperature is 50-70°C. Low-temperature spray drying to obtain probiotic bacteria powder.

4) Tablet

Mix 98% probiotic powder, 1.5% fructooligosaccharide, and 0.5% magnesium stearate uniformly according to the mass percentage, and then use a tablet machine to compress the tablet under a pressure of 4000N to make a probiotic microecological tablet. Wherein, the diameter of the tablet is 8 mm, the tablet weight: 0.3 g, and the hardness: 8 kg/cm ² . The technical indicators of microecological tablets are: the number of lactic acid bacteria ≥ 3 billion cfu/g, the number of yeast ≥ 2 billion cfu/g, and the number of bifidobacteria ≥ 2 billion cfu/g.

Example 2

1) Preparation of probiotic starter

① Preparation of bifidobacteria

Inoculate the frozen Bifidobacterium adolescent powder and Bifidobacterium infantis powder into MRS lactose medium respectively, culture at 37°C for 24 hours, and the microscopic examination conforms to the standard form of Bifidobacterium, and the growth state is good. 5%, were inoculated in MRS lactose medium for activation, 37 ℃, cultured for 20h. Mix Bifidobacterium adolescentis and Bifidobacterium infantis in a volume ratio of 1:1, and save for future use.

② Preparation of Kefir starter

Use skim milk powder to make 10% skim milk solution, after sterilizing at 115°C for 30 minutes, cool to 23°C, inoculate kefir direct-throwing starter at 0.3% of the volume of skim milk, and ferment at 26°C Save for later use after 16 hours.

2) Mixed fermentation

Take concentrated whey protein 18g, glucose 5g, yeast powder 4.29g, calcium carbonate 4.0g, magnesium sulfate 0.3g, manganese sulfate 0.027g, whey powder 10g, inulin 5g, ginseng polysaccharide 1g, soybean oligosaccharide 0.5g, Add distilled water to a total mass of 500 g. After fully dissolving, adjust the pH of the medium to 7.0, and the sterilization conditions are 115°C for 20 minutes, then cool to 25°C for later use.

Inoculate the cooled high-density medium with 5% inoculum, kefir starter: bifidobacteria = 1:1, 30°C, and ferment for 20 hours.

3) Preparation of bacteria powder

Stir the fermented broth evenly, and centrifuge at 4000 rpm for 20 minutes at 15°C to collect concentrated bacteria; add 15% skimmed milk powder and 1% trehalose to the bacteria collected by centrifugation , 7% sodium glutamate, and 5% glycerin, mixed evenly, pre-frozen for 8 hours, and dried in a low-temperature freeze dryer for 24 hours to obtain probiotic powder.

4) Tablet

Mix 96% probiotic bacteria powder, 3% fructooligosaccharide, and 1% magnesium stearate uniformly according to the mass percentage, and then use a tablet machine to compress the tablet under a pressure of 6000N to make a probiotic microecological tablet. Wherein, the diameter of the tablet is 10 mm, the tablet weight: 0.4 g, and the hardness: 9 kg/cm ² . The technical indicators of microecological tablets are: the number of lactic acid bacteria ≥ 3 billion cfu/g, the number of yeast ≥ 2 billion cfu/g, and the number of bifidobacteria ≥ 2 billion cfu/g.

Example 3

1) Preparation of probiotic starter

① Preparation of bifidobacteria

The frozen Bifidobacterium adolescent powder, Bifidobacterium longum powder and Bifidobacterium infantis powder were respectively inoculated in MRS lactose medium at 37°C. After culturing for 24 hours, the standard form of Bifidobacterium was found by microscope inspection. The growth state is good, according to the inoculum size of 6%, inoculated in MRS lactose medium for activation, 37 ℃, cultured for 20h. Mix Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium infantis in a volume ratio of 2:1:1, and store for later use.

② Preparation of Kefir starter

Use skim milk powder to prepare 10% skim milk solution, after sterilizing at 115°C for 20 minutes, cool to 20°C, inoculate 0.5% of the volume of skim milk with kefir starter, and ferment at 28°C Save for later use after 14 hours.

2) Mixed fermentation

Take concentrated whey protein 20g, glucose 8.0g, yeast powder 5g, calcium carbonate 4g, magnesium sulfate 0.1g, manganese sulfate 0.020g, whey powder 10g, inulin 7g, ginseng polysaccharide 0.2g, soybean oligosaccharide 0.1g, Add distilled water to a total mass of 500 g. After fully dissolving, adjust the pH of the medium to 7.0, and the sterilization conditions are 95°C for 30 minutes, then cool to 25°C for later use.

Inoculate the cooled high-density medium with 6% inoculum, kefir starter: bifidobacteria = 3:1, 32°C, and ferment for 18 hours.

3) Preparation of bacteria powder

Stir the fermented broth evenly, and centrifuge at 3000 rpm for 30 minutes at 10°C to collect concentrated bacteria; add 20% skim milk powder and 6% trehalose to the bacteria collected by centrifugation , 5% sodium glutamate, 1% glycerin, mix evenly, adjust the dry matter concentration to 30-40% with sterile water, and carry out under the condition that the air inlet temperature is 80-120°C and the outlet air temperature is 50-70°C. Low-temperature spray drying to obtain probiotic bacteria powder.

4) Tablet

Mix 95% probiotic bacteria powder, 4.7% fructooligosaccharide, and 0.3% magnesium stearate evenly according to the mass percentage, and then use a tablet machine to compress the tablet under a pressure of 8000N to make a probiotic microecological tablet. Wherein, the diameter of the tablet is 12 mm, the tablet weight: 0.5 g, and the hardness: 10 kg/cm ² . The technical indicators of microecological tablets are: the number of lactic acid bacteria ≥ 3 billion cfu/g, the number of yeast ≥ 2 billion cfu/g, and the number of bifidobacteria ≥ 2 billion cfu/g.

Example 4

The probiotic microecological tablet of the present invention can be obtained according to the following materials and process conditions.

1) Preparation of probiotic starter

① Preparation of bifidobacteria

Inoculate the frozen bifidobacteria powder in MRS lactose medium, culture at 37°C for 24 hours, and the microscopic examination conforms to the standard form of bifidobacteria, and the growth state is good. Inoculate 3% to 6% of the inoculum in MRS lactose The culture medium was activated, cultured at 37°C for 18-24 hours and stored for later use.

② Preparation of Kefir starter

Use skim milk powder to prepare 10% skim milk solution, after sterilizing at 90-115°C for 10-30 minutes, cool to 20-30°C, inoculate kefir grains according to 0.1%-0.5% of the volume of skim milk, at 23 Ferment at ~28°C for 14-18 hours and store for later use.

2) Mixed fermentation

Take concentrated whey protein 10-20g, glucose 6-10g, yeast powder 3-5g, calcium carbonate 1-5g, magnesium sulfate 0.05-0.3g, manganese sulfate 0.01-0.05g, whey powder 5-15g, inulin 2.5g ~5g, 0.5~5g ginseng polysaccharide, 0.5~5g soybean oligosaccharide, add distilled water until the total mass is 500g. After fully dissolving, adjust the pH of the medium to 6.5-7.5, and the sterilization conditions are 90-115°C for 10-30 minutes, then cool to 20-30°C for later use.

Inoculate the cooled culture medium with an inoculum amount of 3% to 10%, kefir starter: bifidobacteria = 1 to 3:1, 28 to 36°C, and ferment for 18 to 24 hours to obtain a mixed fermentation broth of probiotics.

3) Preparation of bacteria powder

Stir the prepared fermentation broth evenly, and centrifuge at 10-20°C at a speed of 3000-6000 rpm for 10-30 minutes to collect concentrated bacteria; the bacteria collected by centrifugation are calculated according to the volume of the fermentation broth Add 10% to 15% of skimmed milk powder, 1% to 5% of trehalose, 1% to 5% of sodium glutamate, and 1% to 3% of glycerin, mix well, and freeze-dry at low temperature after pre-freezing or at the inlet temperature Under the conditions of 80-120° C. and an outlet air temperature of 50-70° C., low-temperature spray drying is carried out to obtain probiotic bacteria powder.

4) Tablet

According to the mass percentage, mix 90%-98% probiotic powder, 1%-9% fructooligosaccharide, and 0.1%-1% magnesium stearate evenly, and then use a tablet machine to press under the condition of a pressure of 4000-10000N. Tablets, made into compound probiotic microecological tablets with unit viable bacteria count greater than 6 billion cfu/g. Wherein, the diameter of the tablet is 6-12 mm, the tablet weight: 0.3-0.5 g, and the hardness: 8-10 kg/cm ² .

Claims (10)

1. A probiotic microecological tablet, which is composed of 90% to 98% of probiotic powder, 1% to 9% of fructooligosaccharide, and 0.1% to 1% of magnesium stearate in terms of mass percentage. 2. probiotic micro-ecological tablet according to claim 1, is characterized in that, described probiotic is made up of composite bacterial classification and bifidobacterium in the kefir fermentation agent; By mass kefir fermentation Compound strains in the agent: bifidobacteria = 1 ~ 3: 1. 3. according to claim 1 and 2 described probiotic micro-ecological tablets, it is characterized in that, described probiotic bacterium powder is by the composite strain in kefir starter, bifidobacterium through high-density culture, It is prepared after mixed fermentation, low temperature concentration and drying. 4. according to claim 1 and 2 described probiotic probiotic tablet, it is characterized in that, described bifidobacterium is made of Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium adolescent 1 to 3 of the composition. 5. a preparation method of the probiotic microecological tablet as claimed in claim 1, first use MRS lactose medium to activate bifidobacteria, use skim milk to inoculate kefir grains to prepare kefir starter; its feature That is, it is obtained through mixed fermentation, low-temperature concentration and drying, and mixing and tableting; The mixed fermentation is to prepare the medium according to the following process. The components of the medium by weight percentage include concentrated whey protein 2% to 10%, glucose 1% to 10%, and yeast powder 0.5% to 1%. , calcium carbonate 0.4%~1%, magnesium sulfate 0.01%~0.06%, manganese sulfate 0.001%~0.01%, whey powder 1%~10%, inulin 0.5%~3%, ginseng polysaccharide 0.04%~1%, Soybean oligosaccharides 0.02% ~ 1%, the balance is distilled water; dissolve each component in distilled water, adjust the pH to 6.5 ~ 7.5, keep it at 90 ~ 115 ° C for 10 ~ 30 minutes to sterilize; cool to 20 ~ 30 ° C Inoculate the mixed strains of kefir starter and bifidobacteria according to 3% to 10% of the medium volume, and ferment for 16 to 24 hours at 28 to 36°C to obtain a mixed fermentation broth of probiotics. The inoculation ratio of Phil starter and Bifidobacteria is Kefir starter: Bifidobacteria=1～3:1; The low-temperature concentrated drying is to stir and centrifuge the probiotic mixed fermentation broth for 15-30 minutes under the condition of 3000-6000 r/min, and collect the concentrated bacteria; then add a protective agent to the concentrated bacteria, and vacuum freeze after pre-freezing Drying or low-temperature spray drying under the condition that the inlet air temperature is 80-120°C and the outlet air temperature is 50-70°C, to obtain probiotic bacteria powder; The mixed material pressing is to mix 90%-98% probiotic powder, 1%-9% fructo-oligosaccharide, and 0.1%-1% magnesium stearate evenly according to the mass percentage, and press the tablet to make probiotics Microbial tablets. 6. according to the preparation method of the probiotic micro-ecological tablet described in claim 5, it is characterized in that, described bifidobacterium is activated, is that the bifidobacterium bacterium powder of freezing is inoculated in MRS lactose culture medium, 37 After culturing at ℃ for 24 hours, the microscopic examination conformed to the standard form of bifidobacteria, and the growth state was good. Then inoculate 3% to 6% of the inoculum in MRS lactose medium for activation, and culture at 37℃ for 18 to 24 hours. 7. according to the preparation method of the probiotic microecological tablet described in claim 6, it is characterized in that, described bifidobacterium bacterium powder is bifidobacterium infantis powder, bifidobacterium longum powder, bifidobacterium breve powder Or/and Bifidobacterium adolescentis powder; when using 2-3 kinds of bacteria powder, activate them separately and then mix them to obtain activated Bifidobacterium, which is used for mixed fermentation with kefir starter. 8. according to the preparation method of the probiotic micro-ecological tablet described in claim 5, it is characterized in that, described preparation kefir starter, is to use skim milk powder to be made into 10% skim milk solution, after 90～ After sterilization at 115°C for 10-30 minutes, cool to 20-30°C, inoculate kefir grains at 0.1%-0.5% of the volume of skim milk, and ferment at 23-28°C for 14-18 hours. 9. according to the preparation method of the described probiotic micro-ecological tablet of claim 5, 6 or 8, it is characterized in that, in the low-temperature concentration drying process, described protective agent is based on the mixed fermented liquid quality of probiotics , consisting of 10% to 20% of skimmed milk powder, 1% to 10% of trehalose, 1% to 10% of sodium glutamate, and 1% to 5% of glycerin. 10. According to the preparation method of the probiotic microecological tablet described in claim 5, 6 or 8, it is characterized in that, in the process of mixing and pressing the tablet, the described tablet pressing is under the condition that the pressure is 4000～10000N conduct.