CN103875515B - A kind of blueberry tissue culture outside sprout-cultivating-bottle radication method - Google Patents
A kind of blueberry tissue culture outside sprout-cultivating-bottle radication method Download PDFInfo
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- CN103875515B CN103875515B CN201410054258.XA CN201410054258A CN103875515B CN 103875515 B CN103875515 B CN 103875515B CN 201410054258 A CN201410054258 A CN 201410054258A CN 103875515 B CN103875515 B CN 103875515B
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Abstract
The present invention relates to the breeding method of a kind of blue berry, especially relate to a kind of blueberry tissue culture outside sprout-cultivating-bottle radication method.It mainly solves existing blueberry tissue culture bottle outside sprout-cultivating-bottle radication and there is the technical problem that cultivation program is many, survival rate is low, seedling cost is high.First the present invention selects the blueberry tissue culture Seedling grown fine, carry out seedling exercising domestication, seeding room is based on scattered light, seedling is taken out in bottle, every strain is cut into 24 sections, water of taking root is dipped before cuttage, wherein the substrate of cuttage selects lichen to be layered on seedlings nursing plate, lichen is sterile-processed, use after soaking one day, lichen after immersion clear water is cleaned, wring out and mince, sprinkling moisture frequently during cuttage, water permeable after cuttage, control 80 90%, a water is watered every three days, foliage fertilizer of spray every two weeks, a monthly antibacterial of spray, the blue berry lichen Seedling of stalwartness is obtained after four months, and transplant and plant to nutritive cube.
Description
Technical field
The present invention relates to the breeding method of a kind of blue berry, especially relate to a kind of blueberry tissue culture outside sprout-cultivating-bottle radication method.
Background technology
Blue berry has another name called Pericarpium Citri tangerinae, blue berry, belongs to Ericaceae genus vaccinium plant, for perennial fallen leaves or evergreen shrubs or undershrub.Blueberry contains that anthocyanidin, flavone etc. are multiple has physiologically active ingredient, can prevent cranial nerve aging, cardiac function enhancing, improving eyesight and anticancer etc..Therefore it is classified as one of big health food of the mankind five by international food and agricultural organization.Blue berry has most the fruit tree species of development potentiality by becoming in world wide in this century.Cuttage as one of means most important in asexual propagation, because of the advantage such as it is simple, growing-seedling period is short, breeding coefficient is high and nursery stock is healthy and strong, is used widely at plant seedling-wood breeding.Along with blue berry is understood by people, plantation enthusiasm is the most surging, supply falls short of demand for seedling resource, tissue culture expanding propagation is imperative, owing to blue berry is taken root difficulty during group training, the general mode all taking outside sprout-cultivating-bottle, and in incubation, it is found that blueberry tissue culture rooting exists the problems such as cultivation program is many, survival rate is low, seedling cost is high.
Summary of the invention
The present invention is to provide a kind of blueberry tissue culture outside sprout-cultivating-bottle radication method, and it mainly solves blueberry tissue culture outside sprout-cultivating-bottle radication and there is the technical problem that cultivation program is many, survival rate is low, seedling cost is high.
The above-mentioned technical problem of the present invention is mainly addressed by following technical proposals:
A kind of blueberry tissue culture outside sprout-cultivating-bottle radication method of the present invention, it is characterised in that described method includes:
A. selecting the blueberry tissue culture Seedling grown fine, height is about 10-12 centimetre, and branch is healthy and strong, carries out seedling exercising domestication；
The most first blueberry tissue culture Seedling being placed seeding room, seeding room is based on scattered light, and intensity of illumination is 3000-4000lx, and temperature controls at 15-25 DEG C, after cultivating 5-7 days, opens bottle cap, continues to cultivate 2-3 days in seeding room；
C. seedling is taken out in bottle, every strain is cut into 2-4 section, shears to dip water of taking root before strict sterilization, cuttage, and the cuttage degree of depth is with 1-1.5 centimetre, wherein the substrate of cuttage selects lichen, and process sterilization, and using after soaking one day, the clear water of the lichen after immersion is cleaned, wring out and mince, be layered on seedlings nursing plate；
D. sprinkling moisture frequently during cuttage, to ensure that seedling does not withers；
E. above seedling, a hut is propped up with preservative film, water permeable after cuttage, control about 80%, cultivate canopy of dropping back in 1-2 week, and observe generating state of taking root, water a water every three days, foliage fertilizer of spray every two weeks, a monthly antibacterial of spray, obtains the blue berry lichen Seedling of stalwartness, and transplants and plant to nutritive cube after four months.
The present invention is that blue berry carries out subculture sprout outside sprout-cultivating-bottle technical research, to reduce tissue cultured seedling cultivation link, shorten growing-seedling period, reduce seedling cost, improve that blueberry tissue culture is the most numerous and industrial breeding technique system further, promote that the popularization and application of tissue cultured seedling have important practical significance.
The outside sprout-cultivating-bottle of traditional blueberry tissue culture Seedling, on lichen in terms of cuttage root-taking, blue berry bottle Seedling is cut hop count amount and length is not strict with, sometimes use the not section of cutting or the section of cutting too much, causing the wasting of resources or the highest situation of survival rate to occur, the present invention is carrying out requirement to quantity and the length of the blue berry bottle Seedling section of cutting.
What the present invention strictly controlled blue berry bottle Seedling cuts segment length, cuts segment length at 2.5cm-3cm, and every strain cuts about 3 sections, on the one hand the rooting rate of blue berry bottle Seedling can be made to reach 85%, reduce the quantity of the dead Seedling of weak Seedling；The most maximized improve efficiency, do not waste resource.
As preferably, described blueberry tissue culture Seedling selects growing way healthy and strong, cultivates more than 2 months in tissue culture bottle, but the seedling not less than 3 months.
As preferably, described hydromining IBA of taking root, concentration is 1g/L, and the time of dipping is the 2-3 second, cuts the culture medium on seedling to be removed during Seedling, so as not to mouldy.
As preferably, described step e cultivation temperature is 20 ± 3 DEG C, and intensity of illumination is 2000lx-3000lx, and every day, light application time was about 15 hours.
As preferably, the described lichen in step c processes sterilization with 800 times of carbendazim, shears uses autoclaving, and 121 DEG C of sterilizings 20 minutes wrapped in high-pressure sterilizing pot by shears newspaper, and the carbendazim of 800 times is: take the water dilution gained of the carbendazim 1g 800ml of concentration 50%.
As preferably, in step e, foliage fertilizer is flower piece and BOBOO two kinds of different being used alternatingly of that, and fungicide carbendazim and thiophanate methyl are used alternatingly.BOBOO that is the BOBOO that foliage fertilizer that your plant nutrient center of BOBOO produces；The flower the more foliage fertilizer that flower piece produces for Scotts company.
Therefore, substrate of the present invention with strong points, strict control cut hop count amount and length, and every strain blue berry bottle Seedling cuts about 3 sections, and blue berry Seedling adaptability is good, and efficiency of taking root is high, and survival rate is high；Substrate selects cheap lichen, and water of taking root is homemade, saves than traditional outside sprout-cultivating-bottle Technological Economy；Seedlings nursing plate uses preservative film scaffolding, and moisturizing temperature control is simple, it is simple to operation, environmental Kuznets Curves is simple；Blue berry is taken root substantially, and growing way is healthy and strong.
Detailed description of the invention
Below by embodiment, technical scheme is described in further detail.
Embodiment 1: a kind of blueberry tissue culture outside sprout-cultivating-bottle radication method of this example, the steps include:
A. selecting the blueberry tissue culture Seedling (Lan Yuan) grown fine, height is about 10-12 centimetre, and branch is healthy and strong, carries out seedling exercising domestication；
The most first blueberry tissue culture Seedling being placed seeding room, seeding room is based on scattered light, and intensity of illumination is 3000lx-4000lx, and temperature controls, at 15 DEG C-25 DEG C, after cultivating 5-7 days, to open bottle cap, continues to cultivate 2-3 days in seeding room；
C. seedling is taken out in bottle, every strain is cut into 3 sections, shears to dip water of taking root before strict sterilization, cuttage, and the cuttage degree of depth is advisable with about 1 centimeter, wherein the substrate of cuttage selects lichen, and process sterilization with 800 times of carbendazim, and using after soaking one day, the lichen after soaking is cleaned with clear water, wring out and mince, be layered on seedlings nursing plate；
D. sprinkling moisture frequently during cuttage, to ensure that seedling does not withers；
E. after cuttage, irrigate with water, prop up a hut with preservative film, water permeable after cuttage, to ensure the humidity of growth of seedling environment, control about 80%, cultivate canopy of dropping back in 1-2 week, and observe generating state of taking root, a water, every two weeks foliage fertilizer of spray, monthly antibacterial of spray is handed over every three days, after four months, available healthy and strong blue berry lichen Seedling, can transplant and plant to nutritive cube.
Embodiment 2: a kind of blueberry tissue culture outside sprout-cultivating-bottle radication method of this example, the steps include:
A. selecting the blueberry tissue culture Seedling (Lei Gexi) grown fine, height is about 10-12 centimetre, and branch is healthy and strong, carries out seedling exercising domestication；
The most first blueberry tissue culture Seedling being placed seeding room, seeding room is based on scattered light, and intensity of illumination is 3000lx-4000lx, and temperature controls, at 15 DEG C-25 DEG C, after cultivating 5-7 days, to open bottle cap, continues to cultivate 2-3 days in seeding room；
C. seedling is taken out in bottle, every strain is cut into 3 sections, shears to dip water of taking root before strict sterilization, cuttage, and the cuttage degree of depth is advisable with about 1 centimeter, wherein the substrate of cuttage selects lichen, and process sterilization with 800 times of carbendazim, and using after soaking one day, the lichen after soaking is cleaned with clear water, wring out and mince, be layered on seedlings nursing plate；
D. sprinkling moisture frequently during cuttage, to ensure that seedling does not withers；
E. after cuttage, irrigate with water, prop up a hut with preservative film, water permeable after cuttage, to ensure the humidity of growth of seedling environment, control about 80%, cultivate canopy of dropping back in 1-2 week, and observe generating state of taking root, a water, every two weeks foliage fertilizer of spray, monthly antibacterial of spray is handed over every three days, after four months, available healthy and strong blue berry lichen Seedling, can transplant and plant to nutritive cube.
Embodiment 3: a kind of blueberry tissue culture outside sprout-cultivating-bottle radication method of this example, the steps include:
A. selecting the blueberry tissue culture Seedling (Ao Ni'er) grown fine, height is about 10-12 centimetre, and branch is healthy and strong, carries out seedling exercising domestication；
The most first blueberry tissue culture Seedling being placed seeding room, seeding room is based on scattered light, and intensity of illumination is 3000lx-4000lx, and temperature controls, at 15 DEG C-25 DEG C, after cultivating 5-7 days, to open bottle cap, continues to cultivate 2-3 days in seeding room；
C. seedling is taken out in bottle, every strain is cut into 3 sections, shears to dip water of taking root before strict sterilization, cuttage, and the cuttage degree of depth is advisable with about 1 centimeter, wherein the substrate of cuttage selects lichen, and process sterilization with 800 times of carbendazim, and using after soaking one day, the lichen after soaking is cleaned with clear water, wring out and mince, be layered on seedlings nursing plate；
D. sprinkling moisture frequently during cuttage, to ensure that seedling does not withers；
E. after cuttage, irrigate with water, prop up a hut with preservative film, water permeable after cuttage, to ensure the humidity of growth of seedling environment, control about 80%, cultivate canopy of dropping back in 1-2 week, and observe generating state of taking root, a water, every two weeks foliage fertilizer of spray, monthly antibacterial of spray is handed over every three days, after four months, available healthy and strong blue berry lichen Seedling, can transplant and plant to nutritive cube.
The foregoing is only the specific embodiment of the present invention, but the architectural feature of the present invention is not limited thereto, any those skilled in the art is in the field of the invention, and change or the modification made all are contained among the scope of the claims of the present invention.
Claims (5)
1. a blueberry tissue culture outside sprout-cultivating-bottle radication method, it is characterised in that described method includes:
A. selecting the blueberry tissue culture Seedling grown fine, height is at 10-12 centimetre, and branch is healthy and strong, carries out seedling exercising domestication；
The most first blueberry tissue culture Seedling being placed seeding room, seeding room is based on scattered light, and intensity of illumination is 3000-4000lx, and temperature controls at 15-25 DEG C, after cultivating 5-7 days, opens bottle cap, continues to cultivate 2-3 days in seeding room；
C. seedling is taken out in bottle, every strain is cut into 2-4 section, cut segment length at 2.5cm-3cm, shears uses autoclaving, 121 DEG C of sterilizings 20 minutes are wrapped in high-pressure sterilizing pot with newspaper, water of taking root is dipped before cuttage, the cuttage degree of depth is 1-1.5 centimetre, wherein the substrate of cuttage selects lichen, and lichen processes sterilization with 800 times of carbendazim, and the carbendazim of 800 times is: take the water dilution gained of the carbendazim 1g 800ml of concentration 50%, use after soaking one day, lichen after immersion clear water is cleaned, and wrings out and minces, is layered on seedlings nursing plate；
D. sprinkling moisture frequently during cuttage, to ensure that seedling does not withers；
E. above seedling, a hut is propped up with preservative film, water permeable after cuttage, control about 80%, cultivate canopy of dropping back in 1-2 week, a water, every two weeks foliage fertilizer of spray, monthly antibacterial of spray is watered every three days, obtain the blue berry lichen Seedling of stalwartness after four months, and transplant and plant to nutritive cube.
A kind of blueberry tissue culture outside sprout-cultivating-bottle radication method the most according to claim 1, it is characterised in that described blueberry tissue culture Seedling selects growing way healthy and strong, cultivates more than 2 months in tissue culture bottle, but the seedling not less than 3 months.
A kind of blueberry tissue culture outside sprout-cultivating-bottle radication method the most according to claim 1, it is characterised in that described hydromining IBA of taking root, concentration is 1g/L, and the time of dipping is the 2-3 second, cuts the culture medium on seedling to be removed during Seedling.
A kind of blueberry tissue culture outside sprout-cultivating-bottle radication method the most according to claim 1, it is characterised in that described step e cultivation temperature is 20 ± 3 DEG C, and intensity of illumination is 2000lx-3000lx, and every day, light application time was 15 hours.
A kind of blueberry tissue culture outside sprout-cultivating-bottle radication method the most according to claim 1, it is characterised in that in described step e, foliage fertilizer is flower piece and BOBOO two kinds of different being used alternatingly of that, and fungicide carbendazim and thiophanate methyl are used alternatingly.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104429849B (en) * | 2014-11-13 | 2016-10-05 | 大连大学 | A kind of new method for water planting blueberry |
| CN105075777B (en) * | 2015-08-24 | 2017-07-07 | 山东省烟台市农业科学研究院 | A kind of blueberry liver moss seedling mating system |
| CN106613859A (en) * | 2017-02-08 | 2017-05-10 | 合肥师范学院 | Cultivation method of sound seedlings of blueberry tissue culture seedlings |
| CN108848977A (en) * | 2017-12-20 | 2018-11-23 | 江苏省林业科学研究院 | A kind of promotion blueberry tissue-cultured seedling rooting method |
| CN108668659A (en) * | 2018-07-18 | 2018-10-19 | 苏州枫彩生态科技集团有限公司 | A kind of method of the outer cuttage root-taking of blueberry bottle |
| CN110786157A (en) * | 2018-11-23 | 2020-02-14 | 江苏省林业科学研究院 | Method for promoting rooting of ilex verticillata tissue culture seedlings |
| CN109349117B (en) * | 2018-12-17 | 2021-07-02 | 江苏省中国科学院植物研究所 | Blueberry tissue culture seedling ex-vitro rooting method |
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