CN103860423B - A kind of have collutory of anti-inflammation deodorize and its production and use - Google Patents
A kind of have collutory of anti-inflammation deodorize and its production and use Download PDFInfo
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Abstract
The invention discloses and a kind of there is collutory of anti-inflammation deodorize and its production and use, it is with Pericarpium Granati extract, Flos Rosae Rugosae, Calyx Seu Fructus Physalis is primary raw material, with wetting agent, sweeting agent, antiseptic and distilled water formulated, this collutory is by the combined effect of various active composition, obstruction can be caused at dental surface, antibacterial is made to be difficult to stick to dental surface, reduce and be attached to dental surface and the antibacterial causing dental plaque, thus play maintenance oral cavity cleaning, remove halitosis, can be used for prevention or treatment gingivitis, periodontitis, oral ulcer and oral cavity and laryngopharyngitis, halitosis can be eliminated simultaneously, keep breath freshening, both can be used for the auxiliary treatment of oral inflammation, also can be used for regular oral health routine, life-time service can not produce any side effect, utilizes rinsing the mouth action, be not only conducive to the motion of oral cavity Surrounding muscles when this product uses, can also promote the self-cleaning action in oral cavity, be the optimum selection keeping oral hygiene, control oral cavity infection.
Description
Technical field
The present invention relates to and a kind of there is collutory of anti-inflammation deodorize and its production and use, particularly relate to and a kind of there is anti-inflammation, deodorize, liquid oral collutory easy to use, without any side effects and its production and use.
Background technology
Oral cavity is one of approach that pathogenic microorganism invades human body, and the temperature in oral cavity, humidity and food debris, suitable micro-organisms flourish, intraoral diastema, gums groove are difficult to clean, and these anatomical features are conducive to bacterial growth.So the health of oral hygiene to human body is very important, the patient of especially high heat, postoperative and disturbance of consciousness, artificial respiration, because of can not oral feeding, salivation reduces, xerostomia, oral cavity self-purification capacity declines, and causes bacterial reproduction power to strengthen, and, because oral secretion can not be swallowed, remain in oral cavity, become the culture medium that antibacterial is good, these may become the reason of dental caries, periodontal abscess, also may cause bronchitis, pneumonia.In addition, because saliva etc. exists in this special environment of oral cavity, under constant humidity, temperature and nutrition, bacterial reproduction grows, and all parasitic microorganisms time normal, when our resistance declines, they are just active pathogenic, make oral cavity bacterium amount reproduction, carbohydrate breakdown, fermentation, acid production strengthen, and thus not only easily cause periodontal disease, parotitis etc., and oral cavity can be made smelly, affect appetite and digestive function, cause oral cavity infection, even cause systemic infection.According to the display of Chinese oral cavity health association authority statistical data, China's oral disease prevalence is up to 97.6%, and almost everybody has oral disease in various degree, as diseases such as toothache, gingivitis, periodontitis, gingival hemorrhages.
Oral health is the symbol of national society's civilization, and oral health has been classified as the important indicator evaluating level of human health by World Health Organization (WHO).Along with science and civilized development, the raising of people's quality of life, everybody strengthens gradually to the consciousness of oral cavity health, and mouth care seems particularly important.Although the importance of mouth care obtains accreditation at home, the degree of knowledge and value is still inadequate.Mouth care is prevention disease in mouth, and keeping, promote healthy, to improve rehabilitation patients ' life quality (QQL) science and technology, is keep oral cavity cleaning, one of prophylactic means.Mouth care can make oral cavity bacterium number reduce, due to mechanical stimulus, promote salivation, strengthen oral cavity from resulting net force, thus keep oral cavity cleaning, moistening, make patient comfort, salubrious, the complication such as prevention of oral infections, prevent halitosis, sordes, promote appetite, safeguard the normal function in oral cavity.Mouth care has not been simple oral cavity cleaning, and mouth care prevents disease from simple, develops into maintenance, promotes healthy, improve the science and technology of patients ' life quality.Medicament such as buccal tablet, chewing gum, diaphragm etc. at present for oral disease and mouth care are difficult to penetrate in teeth space, due therapeutical effect can not be played, and the preparation used adopts crude extract to make mostly, active constituent content is low and unstable, is difficult to reach set effect.
Pericarpium Granati system Uygur medicine medicinal herbs most in use are the dry peel of Punicaceae plant Punica granatum L. (punicagranatumL.).Pericarpium Granati acid, puckery, be mainly used in chronic diarrhea, the card such as hemorrhage in China's traditional medicine.Polyphenol in Pericarpium Granati is the general name that a class has polyphenol hydroxyl compound, comprise elladitannin, Galla Turcica (Galla Helepensis) tannin, ellagic acid, the multiple compounds such as gallic acid, content is about the 10%-20% of its dry mass, have antibacterial, antioxidation, defying age, anti-cancer and cancer-preventing, multiple physiology and the pharmacologically actives such as blood pressure lowering and prevention cardiovascular and cerebrovascular disease, but polyphenols is unstable, temperature is high, heated time is long oxidizable, cause extraction ratio low, activity decrease, therefore adopted by Pericarpium Granati the concentrated and macroporous resin adsorption of extract at low temperature to obtain polyphenol effective site, improve stability and the extraction ratio of polyphenol components, this position counts more than 60% containing total polyphenols with gallic acid.Because EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL is containing a large amount of aldehydes matter, poorly soluble in water, add a certain amount of wetting agent and stir, its dissolubility in water can be improved.The present invention is on the basis of 200910113497.7 pomegranate peel polyphenol anti-inflammation effervescent tablets and its production and use, further studies the purposes of Pericarpium Granati extract.
Flos Rosae Rugosae is recorded in Chinese Pharmacopoeia version in 2010 and Uygur's medical material standard first volume, is Uygur medicine medicinal herbs most in use, has detumescence, hemostasis, pain relieving, heat clearing away heart tonifying, moistens the effect such as just, have that heat clearing away is quenched one's thirst, vital energy regualting and blood circulation-promoting, suitable gas skin care effect.Flos Rosae Rugosae is containing volatile oil, and in oil, main component is citronellol, Geraniol, nerol, eugenol etc.Containing abundant Flavonoid substances in Flos Rosae Rugosae, this compounds has higher antioxidant activity, can effectively scavenging free radicals, removing pigmentation.In addition, still containing Quercitroside, tannin, fatty oil, organic acid etc.Total flavones compound in our systematic study Flos Rosae Rugosae in early stage, found that, when ethanol is for extracting solvent, Flavonoid substances in Flos Rosae Rugosae can be extracted completely.
Calyx Seu Fructus Physalis is the dry Constellation calyx of plant of Solanaceae Calyx seu fructus physalis PhysalisalkekengiL.var.franchetii (mast.) Makino or the Constellation calyx of band fruit.Its nature and flavor are sweet, sour, cold, there is heat clearing away, removing toxic substances, diuresis, clears phlegm, be used for the treatment of that pharyngalgia is silent, phlegm-heat cough, dysuria, external treatment pemphigus, eczema etc.Be usually used in acute, suppurative tonsillitis and laryngopharynx swelling and pain clinically.Calyx Seu Fructus Physalis contains several large class chemical compositions such as lactone, flavonoid, terpenoid, alkaloids, sterols, amino acids and inorganic elements, as physalin compounds, luteoloside etc., has clear and definite antibacterial, antiinflammatory, antiallergic and analgesia isoreactivity.Bibliographical information, Calyx Seu Fructus Physalis antibiotic effective ingredient is tentatively thought in its oily liquid, and this part is in vitro to bacillus pyocyaneus, and staphylococcus aureus has obvious inhibitory action.Chinese Pharmacopoeia version in 2010 Calyx Seu Fructus Physalis recorded is that contrast carries out assay to it with luteoloside.When adopting different extraction solvent to extract Calyx Seu Fructus Physalis, its content has bigger difference.Result of the test shows, when water is for extracting solvent, in Calyx Seu Fructus Physalis, the content of luteoloside is 0.08%; And when adopting ethanol to extract, the content of luteoloside, more than 0.14%, illustrates that in Calyx Seu Fructus Physalis, Flavonoid substances luteoloside has good dissolubility at ethanol.
In the present invention, Flos Rosae Rugosae and Calyx Seu Fructus Physalis adopt united extraction, and with flavone compound luteoloside content for inspection target, investigate the ethanol of water and variable concentrations respectively as extraction solvent, when result concentration of alcohol is more than 60%, luteoloside content is higher.In preliminary experiment, when Flos Rosae Rugosae and Calyx Seu Fructus Physalis adopt water and low-concentration ethanol to extract, there is particle in extracting solution, affect the clarity of solution.Collutory of the present invention is oral solution, supernatant liquid preparation is should be by States Pharmacopoeia specifications, for improving clarity and the stability of collutory, with solution appearance character, mouthfeel, uniformity/stability for index, investigate the addition sequence of the kind of adjuvant, consumption and each component, filter out best prescription proportioning.
In collutory on existing market, majority is that Integrated TCM carrys out prevention and therapy oral disease, and be main composition with Western medicine, though have good bacteriostasis, but Western medicine side effect produces drug resistance greatly, easily, there is the not good and adverse effect of mouthfeel, severe patient even may cause dysbacteriosis, oral mucosa strips off and superposition infects; Some mouth care solution containing medicinal herb components are the proper superiority showing oneself in this regard then.The present invention utilizes medicinal and edible plant to make pure Chinese medicine gargle for primary raw material, by the combined effect of various active composition, through antibacterial activity in vitro, anti-inflammatory activity research, result shows the activity of staphylococcus aureus and Hemolytic streptococcus comparatively strong, can alleviate the inflammatory edema caused by dimethylbenzene.Be applicable to the oral diseases such as oral ulcer, periodontitis, gingivitis, gingival hemorrhage, halitosis, pharyngolaryngitis, tonsillitis.
Summary of the invention
The object of this invention is to provide and a kind of there is collutory of anti-inflammation deodorize and its production and use, this collutory is formulated with EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL, Flos Rosae Rugosae, Calyx Seu Fructus Physalis, wetting agent, sweeting agent, antiseptic and distilled water, this collutory can cause obstruction at dental surface, antibacterial is made to be difficult to stick to dental surface, reduce and be attached to dental surface and cause the antibacterial of dental plaque, thus play and keep oral cavity cleaning, remove halitosis, can be used for prevention or treatment gingivitis, periodontitis, oral ulcer and oral cavity and laryngopharyngitis; Can halitosis be eliminated simultaneously, keep breath freshening.Both can be used for the auxiliary treatment of oral inflammation, and also can be used for regular oral health routine, life-time service can not produce any side effect, utilizes rinsing the mouth action, be not only conducive to the motion of oral cavity Surrounding muscles, can also promote the self-cleaning action in oral cavity when this product uses.Rinsing the mouth method is simple, convenient, is easy to be accepted by patient and implement, and is the optimum selection keeping oral hygiene, control oral cavity infection.
A kind of collutory with anti-inflammation deodorize of the present invention, this collutory be by percentage by weight be EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 5-20%, Flos Rosae Rugosae 10-30%, Calyx Seu Fructus Physalis 10-30%, wetting agent be glycerol or propylene glycol 5-20%, sweeting agent is saccharin sodium, cyclamate or aspartame 0.5-2%, antiseptic are sodium benzoate or potassium sorbate 0.1-1%, surplus is that distilled water is made.
The described preparation method with the collutory of anti-inflammation deodorize, follows these steps to carry out:
A, get Pericarpium Granati and be ground into coarse powder, adding 10-20 times amount concentration is 40% ethanol, extracts 1-3 time, each 1-3 hour under temperature 71-85 DEG C of condition, and filter, merging filtrate decompression recycling ethanol, is concentrated into the concentrated solution that every mL is equivalent to crude drug 0.15-0.2g; Concentrated solution is joined on processed good HPD300 type macroporous adsorptive resins, absorption 3-12 hour, first with deionized water rinsing to effluent paler colour time, again with 60% ethanol elution of 2-5 times of column volume, collect containing alcohol eluen, reclaim ethanol, concentrating under reduced pressure vacuum drying, pulverize, cross 80-120 mesh sieve, obtain EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL for subsequent use;
B, get Flos Rosae Rugosae 10-30%, Calyx Seu Fructus Physalis 10-30% add 5-20 times amount concentration be 65%-90% alcohol heating reflux extract 1-4 time, each 1-3 hour, filters, merging filtrate decompression recycling ethanol, be concentrated into the solution that relative density is 1.05-1.10 temperature 60 C, for subsequent use;
C, getting EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 5-20% in step a, to be dissolved in wetting agent be in glycerol or propylene glycol 5-20%, stir, join in the solution of step b, add that sweeting agent is saccharin sodium simultaneously, cyclamate or aspartame 0.5-2%, antiseptic are sodium benzoate or potassium sorbate 0.1-1% and surplus distilled water, abundant stirring and evenly mixing, leave standstill filtration after 24 hours, packaging, fill, the collutory of anti-inflammation deodorize must be had.
The purposes of the described collutory with anti-inflammation deodorize in the medicine of preparation prevention or treatment gingivitis, periodontitis, oral ulcer and oral cavity and laryngopharyngitis.
Purposes in the medicine of the described collutory with anti-inflammation deodorize in preparation auxiliary treatment oral inflammation or regular oral health routine.
Of the present invention a kind ofly have collutory of anti-inflammation deodorize and its production and use, it is for primary raw material with EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL, Flos Rosae Rugosae, Calyx Seu Fructus Physalis, formulated with wetting agent, sweeting agent, antiseptic and water, this product is by the combined effect of various active composition, can suppress, remove oral cavity bacterium, prevent or treat gingivitis, periodontitis, oral ulcer and oral cavity and laryngopharyngitis, simultaneously, eliminate halitosis, keep breath freshening.This collutory is made up of pure Chinese medicine, not only has whole effects of general collutory, and to human body without any side effect.The feature of this product is: raw material is easy to get, prepare simple, safety is good, also can not damage patient even if swallow.Formula provided by the invention has good antibacterial action, can be used for collutory, tablet, granule, membrane, chewing gum, effervescent formulation etc., not only may be used for the auxiliary treatment of oral inflammation, also can be used for regular oral health routine, be suitable for large-scale promotion clinically.
Accompanying drawing explanation
Fig. 1 is luteoloside canonical plotting of the present invention;
Fig. 2 is Extraction solvent result figure of the present invention.
Detailed description of the invention
Embodiment 1(percentage by weight)
A, get Pericarpium Granati and be ground into coarse powder, adding 10 times amount concentration is 40% ethanol warm macerating, and temperature 71 DEG C is extracted 3 times, each 1 hour, and filter, decompression filtrate recycling ethanol, is concentrated into every mL and is equivalent to crude drug 0.15-0.2g; By concentrated solution through HPD300 macroporous resin chromatography, absorption 12h, is first eluted to effluent with distilled water closely colourless, then uses 2 times of column volume 60% ethanol elutions, and collect containing alcohol eluen, reclaim ethanol, concentrating under reduced pressure dry, pulverize, and crosses 80 mesh sieves, for subsequent use;
B, get Flos Rosae Rugosae 10%, Calyx Seu Fructus Physalis 10%, adding 5 times amount concentration is that 65% alcohol heating reflux extracts 2 times, each 2 hours, and filter, merging filtrate decompression recycling ethanol, is concentrated into the solution that relative density is 1.05-1.10 (temperature 60 C), for subsequent use;
C, get EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 5% in step a and be dissolved in wetting agent propylene glycol 5%, stir, join step b concentrate after solution in, add sweeting agent saccharin sodium 0.5%, preservative sodium benzoate 0.1% and surplus distilled water simultaneously, abundant stirring and evenly mixing, leave standstill filtration after 24 hours, packaging, fill, the collutory of anti-inflammation deodorize must be had.
Embodiment 2(percentage by weight)
A, get Pericarpium Granati and be ground into coarse powder, adding 15 times amount concentration is 40% ethanol warm macerating, and temperature 75 DEG C is extracted 2 times, each 2 hours, and filter, decompression filtrate recycling ethanol, is concentrated into every mL and is equivalent to crude drug 0.15-0.2g; By concentrated solution through HPD300 macroporous resin chromatography, absorption 12h, is first eluted to effluent with distilled water closely colourless, then uses 3 times of column volume 60% ethanol elutions, and collect containing alcohol eluen, reclaim ethanol, concentrating under reduced pressure dry, pulverize, and crosses 100 mesh sieves, for subsequent use;
B, get Flos Rosae Rugosae 20%, Calyx Seu Fructus Physalis 20%, adding 10 times amount concentration is that 70% alcohol heating reflux extracts 1 time, extracts 3 hours, and filter, merging filtrate decompression recycling ethanol, is concentrated into the solution that relative density is 1.05-1.10 (temperature 60 C), for subsequent use;
C, get EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 10% in step a and be dissolved in wetting agent propylene glycol 10%, stir, join step b concentrate after solution in, add sweeting agent cyclamate 0.8%, antiseptic cyclamate 0.8% and surplus distilled water simultaneously, abundant stirring and evenly mixing, leave standstill filtration after 24 hours, packaging, fill, the collutory of anti-inflammation deodorize must be had.
Embodiment 3(percentage by weight)
A, get Pericarpium Granati and be ground into coarse powder, adding 20 times amount concentration is 40% ethanol, extracts 3 times, each 1.5 hours under temperature 80 DEG C of conditions, and filter, merging filtrate decompression recycling ethanol, is concentrated into every mL and is equivalent to crude drug 0.15-0.2g; Concentrated solution is joined on processed good HPD300 type macroporous adsorptive resins, adsorb 6 hours, first with deionized water rinsing to effluent paler colour time, then use 60% ethanol elution of 4 times of column volumes, collect containing alcohol eluen, reclaim ethanol, concentrating under reduced pressure vacuum drying becomes dry extract, pulverizes, and crosses 120 mesh sieves, obtain EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL, for subsequent use;
B, get Flos Rosae Rugosae 15%, Calyx Seu Fructus Physalis 20%, adding 15 times amount concentration is that 75% alcohol heating reflux extracts 3 times, each 2 hours, and filter, merging filtrate decompression recycling ethanol, is concentrated into the solution that relative density is 1.05-1.10 temperature 60 C, for subsequent use;
C, getting EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 15% in step a, to be dissolved in wetting agent be in glycerol 10%, stir, join in the solution of step b, add that sweeting agent is aspartame 0.6%, antiseptic is sodium benzoate 0.3% and surplus distilled water simultaneously, abundant stirring and evenly mixing, leave standstill filtration after 24 hours, packaging, fill, the collutory of anti-inflammation deodorize must be had.
Embodiment 4(percentage by weight)
A, get Pericarpium Granati and be ground into coarse powder, adding 18 times amount concentration is 40% ethanol, extracts 3 times, each 3 hours under temperature 85 DEG C of conditions, and filter, merging filtrate decompression recycling ethanol, is concentrated into every mL and is equivalent to crude drug 0.15-0.2g; Concentrated solution is joined on processed good HPD300 type macroporous adsorptive resins, adsorb 3 hours, first with deionized water rinsing to effluent paler colour time, then use 60% ethanol elution of 3 times of column volumes, collect containing alcohol eluen, reclaim ethanol, concentrating under reduced pressure vacuum drying becomes dry extract, pulverizes, and crosses 80 mesh sieves, obtain EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL, for subsequent use;
B, get Flos Rosae Rugosae 20%, Calyx Seu Fructus Physalis 30%, adding 20 times amount concentration is that 80% alcohol heating reflux extracts 4 times, each 1 hour, and filter, merging filtrate decompression recycling ethanol, is concentrated into the solution that relative density is 1.05-1.10 temperature 60 C, for subsequent use;
C, getting EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 20% in step a, to be dissolved in wetting agent be in glycerol 20%, stir, join in the solution of step b, add that sweeting agent is cyclamate 1%, antiseptic is potassium sorbate 0.5% and surplus distilled water simultaneously, abundant stirring and evenly mixing, leave standstill filtration after 24 hours, packaging, fill, the collutory of anti-inflammation deodorize must be had.
Embodiment 5(percentage by weight)
A, get Pericarpium Granati and be ground into coarse powder, adding 14 times amount concentration is 40% ethanol, extracts 2 times, each 3 hours under temperature 80 DEG C of conditions, and filter, merging filtrate decompression recycling ethanol, is concentrated into every mL and is equivalent to crude drug 0.15-0.2g; Concentrated solution is joined on processed good HPD300 type macroporous adsorptive resins, adsorb 9 hours, first with deionized water rinsing to effluent paler colour time, then use 60% ethanol elution of 5 times of column volumes, collect containing alcohol eluen, reclaim ethanol, concentrating under reduced pressure vacuum drying becomes dry extract, pulverizes, and crosses 100 mesh sieves, obtain EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL, for subsequent use;
B, get Flos Rosae Rugosae 30%, Calyx Seu Fructus Physalis 20%, adding 15 times amount concentration is that 90% alcohol heating reflux extracts 2 times, each 2 hours, and filter, merging filtrate decompression recycling ethanol, is concentrated into the solution that relative density is 1.05-1.10 temperature 60 C, for subsequent use;
C, get EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 5% in step a and be dissolved in wetting agent propylene glycol 5%, stir, join in the solution of step b, add that sweeting agent is saccharin sodium 0.8%, antiseptic is sodium benzoate 0.8% and surplus distilled water simultaneously, abundant stirring and evenly mixing, leave standstill filtration after 24 hours, packaging, fill, the collutory of anti-inflammation deodorize must be had.
Embodiment 6 a kind of purposes with anti-inflammation deodorize collutory of the present invention:
The mensuration of Flos Rosae Rugosae and Calyx Seu Fructus Physalis luteoloside in described collutory formula:
Measure according to high performance liquid chromatography (" Chinese Pharmacopoeia " version in 2010, annex VID);
Chromatographic condition and system suitability take octadecylsilane chemically bonded silica as filler; Take acetonitrile as mobile phase A, with 0.2% phosphoric acid solution for Mobile phase B, the regulation according to the form below carries out gradient elution; Determined wavelength is 350nm.Number of theoretical plate calculates should be not less than 3000 by luteoloside peak;
The preparation of reference substance solution: get luteoloside reference substance appropriate, accurately weighed, add methanol and make the solution of every 1mL containing 49.2 μ g, to obtain final product;
Prepared by standard curve: accurate absorption luteoloside reference substance solution 4 μ L, 8 μ L, 10 μ L, 12 μ L, 15 μ L respectively, by above-mentioned chromatographic condition sample introduction, with sample introduction concentration (C) for abscissa, peak area (Y) returns for vertical coordinate, obtaining standard curve equation is Y=32336C+77398, r
2=0.999(is shown in Fig. 1), result shows, regression equation luteoloside peak area and sample introduction concentration within the scope of 19.68 μ g/mL ~ 73.8 μ g/mL have good linear relationship;
The preparation of need testing solution: get Flos Rosae Rugosae and Calyx Seu Fructus Physalis adopts the alcoholic solution of water and variable concentrations to extract respectively, extracting solution filters through microporous filter membrane (0.45 μm), gets subsequent filtrate by above-mentioned chromatographic condition sample introduction, the results are shown in Figure 2.
Embodiment 7
The recipe determination of described collutory:
EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL solution: get EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 5g, is dissolved in 10ml propylene glycol, stirs, add water to 50ml;
Rose extract: get Flos Rosae Rugosae 10g, adds 60% ethanol extraction 2 times, each 2 hours, and filter, merging filtrate, is concentrated into 50ml;
Calyx Seu Fructus Physalis extracting solution: get Calyx Seu Fructus Physalis 10g, adds 80% ethanol extraction 2 times, each 2 hours, and filter, merging filtrate, is concentrated into 50ml;
Collutory: get Flos Rosae Rugosae, each 10g of Calyx Seu Fructus Physalis, add 70% ethanol extraction 2 times, each 2 hours, filter, merging filtrate, decompression recycling ethanol, is concentrated into the solution that relative density is 1.05-1.10 temperature 60 C, for subsequent use; Separately get EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL 5g, be dissolved in 10ml propylene glycol, stir, join in above-mentioned solution, abundant stirring and evenly mixing, adds water to 100ml;
Antibacterial experiment in vitro:
The preparation of the medicinal scraps of paper: select the filter paper that water absorbing force is strong and homogeneous, the disk of 6mm is broken into puncher, be sub-packed in clean dried in vitro, put into high-pressure sterilizing pot xeothermic (temperature 120 DEG C) sterilizing after 2 hours, taking-up is put into thermostatic drying chamber and is carried out drying, get dried scraps of paper some, put into different solutions, for subsequent use after infiltrating;
Extracorporeal bacteria inhibitor test-adopt paper disk method: staphylococcus aureus (SA), escherichia coli (EC) are inoculated in nutrient agar slopes respectively, cultivate 18-24 hour, make bacteria suspension, Maxwell turbidimetry for Determination bacterial concentration, makes it 10
6-10
7cfu/ml.0.1ml bacterium liquid is coated nutrient agar panel respectively, each strain does 3 Duplicate Samples, to the scraps of paper flicking of various medicinal liquid be contained on flat board after half a minute, negative control is done with the blank scraps of paper, antibacterial is taken out after temperature 37 DEG C of constant temperature culture 18h, by measuring inhibition zone size, judge that tested strain is to medicinal liquid sensitivity, the results are shown in Table 1:
The different extracting solution extracorporeal bacteria inhibitor test of table 1
Embodiment 8
The In Vitro Bacteriostasis of described collutory, antiinflammatory experiment:
The preparation of the medicinal scraps of paper: select the filter paper that water absorbing force is strong and homogeneous, the disk of 6mm is broken into puncher, be sub-packed in clean dried in vitro, put into high-pressure sterilizing pot xeothermic (temperature 120 DEG C) sterilizing after 2 hours, taking-up is put into thermostatic drying chamber and is carried out drying, get dried scraps of paper some, put into collutory, for subsequent use after infiltrating;
Extracorporeal bacteria inhibitor test-adopt paper disk method: staphylococcus aureus, escherichia coli, Candida albicans, Streptococcus mutans are inoculated in respectively Nutrient agar and blood agar inclined-plane, cultivate 18-24 hour, make bacteria suspension, Maxwell turbidimetry for Determination bacterial concentration, makes it 10
6-10
7cfu/ml.0.1ml bacterium liquid is coated Nutrient agar and blood agar plate respectively, each strain does 3 Duplicate Samples, to the scraps of paper flicking of various medicinal liquid be contained on flat board after half a minute, negative control is done with the blank scraps of paper, antibacterial is taken out after temperature 37 DEG C of constant temperature culture 18h, by measuring inhibition zone size, judge that tested strain is to medicinal liquid sensitivity, the results are shown in Table 2:
Table 2 bacteriostatic experiment result
The criterion of antibacterials sensitivity is: antibacterial circle diameter >=15mm is extremely sensitive, and 10-15mm is medium sensitivity, and < 10mm is drug resistance.
Mice ear is tested:
KM mice 50, male and female half and half, body weight 18-22g, by the weight of animals, sex be divided at random NS matched group, positive control drug Dobell's solution (0.1ml/ only), basic, normal, high three the dosage groups of collutory (0.1ml/ only, 0.2ml/ only, 0.4ml/ only), often organize 10, each administration group mice coating every day once, be applied to auris dextra, connect painting 5 days, blank group is coated with the distilled water of equivalent, after last coating 30min, each administration group washes away medicinal liquid with distilled water, clean with cotton balls, be only coated with dimethylbenzene 0.1ml/ to mouse right ear, left ear is contrast.After 30min, the neck that broken by mice is put to death, and with the card punch of diameter 8mm, ears same area is respectively laid a slice, weighs respectively with electronic balance, using the difference of left and right auricle weight as swelling, calculate each group of swelling degree, obtain swelling rate, the results are shown in Table 3:
The impact of table 3 dimethylbenzene induced mice ear swelling experiment
Remarks: compare with matched group,
﹡p<0.05,
﹡ ﹡p < 0.01, p>0.05
As can be seen from Table 3, administration is after 5 days, in collutory, dosage group (0.2ml/ only), high dose group (0.4ml/ only) have the effect significantly suppressing mice auricle swelling, ear swelling value is all lower than NS group, difference has significance (p<0.05 or p<0.01), show that collutory of the present invention has significant antiinflammatory action, positive control Dobell's solution also has same purpose.
Claims (3)
1. one kind has the collutory of anti-inflammation deodorization functions, it is characterized in that this collutory is is Pericarpium Granati extract 5-20%, Flos Rosae Rugosae 10-30%, Calyx Seu Fructus Physalis 10-30% by percentage by weight, the wetting agent of 5-20% is glycerol or propylene glycol, the sweeting agent of 0.5-2% is saccharin sodium, cyclamate or aspartame, the antiseptic of 0.1-1% is sodium benzoate or potassium sorbate, and surplus is that distilled water is formulated.
2. the collutory with anti-inflammation deodorization functions according to claim 1 prevents in preparation or treats the purposes in the medicine of gingivitis, periodontitis, oral ulcer and laryngopharyngitis.
3. the purposes of collutory in the medicine preparing auxiliary treatment oral inflammation or regular oral health routine with anti-inflammation deodorization functions according to claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310648219.8A CN103860423B (en) | 2012-12-04 | 2013-12-03 | A kind of have collutory of anti-inflammation deodorize and its production and use |
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| CN201210513345.8 | 2012-12-04 | ||
| CN2012105133458A CN102940595A (en) | 2012-12-04 | 2012-12-04 | Mouthwash with functions of antibiosis, antiphlogosis and deodorization, and preparation method and use thereof |
| CN201310648219.8A CN103860423B (en) | 2012-12-04 | 2013-12-03 | A kind of have collutory of anti-inflammation deodorize and its production and use |
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| CN201310648219.8A Active CN103860423B (en) | 2012-12-04 | 2013-12-03 | A kind of have collutory of anti-inflammation deodorize and its production and use |
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| CN102940595A (en) * | 2012-12-04 | 2013-02-27 | 中国科学院新疆理化技术研究所 | Mouthwash with functions of antibiosis, antiphlogosis and deodorization, and preparation method and use thereof |
| CN106177923B (en) * | 2016-07-12 | 2020-08-18 | 方雅悯 | Oral ulcer film agent and preparation method thereof |
| CN106237337B (en) * | 2016-08-05 | 2019-11-08 | 河南中帅医药科技股份有限公司 | A kind of liquid pharmaceutical formulation anticorrosive composite |
| CN107595696B (en) * | 2017-09-26 | 2020-12-15 | 昆明理工大学 | Mouthwash for preventing and treating periodontitis and preparation method thereof |
| CN107693430A (en) * | 2017-11-08 | 2018-02-16 | 江西草珊瑚消毒用品有限公司 | Draft nurses one's health antibacterial gargle |
| CN108379152A (en) * | 2018-04-16 | 2018-08-10 | 新乡医学院三全学院 | A kind of oral nursing liquid and preparation method |
| CN110357900A (en) * | 2019-08-02 | 2019-10-22 | 中国科学院新疆理化技术研究所 | A kind of intermediate processing obtaining ellagic acid from pomegranate rind extract |
| CN111346151A (en) * | 2020-04-10 | 2020-06-30 | 广东药科大学 | Application of calyx seu fructus physalis extract in preparing medicine for treating oral ulcer |
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| CN1059095C (en) * | 1995-09-21 | 2000-12-06 | 侯逢春 | Medicine for treatment of infectious oral disease |
| JP2009167122A (en) * | 2008-01-16 | 2009-07-30 | Hiroshi Fukutomi | Gargle |
| US8114448B2 (en) * | 2008-01-21 | 2012-02-14 | Himalaya Global Holdings Limited | Herbal dental care composition, method of manufacturing the same and use thereof |
| CN101283962A (en) * | 2008-05-30 | 2008-10-15 | 美晨集团股份有限公司 | Chinese herbal medicine mouthwash containing Chinese herbal medicine active composition for resisting helicobacter pylori and preparing method thereof |
| DE102009050407A1 (en) * | 2009-10-22 | 2011-06-22 | Bionorica SE, 92318 | Anti-inflammatory agent containing hydrolysates from plant extracts |
| CN101700257B (en) * | 2009-10-26 | 2011-10-19 | 中国科学院新疆理化技术研究所 | Pomegranate peel polyphenol antibiosis and antiphlogosis effervescent tablet as well as preparation method and application thereof |
| AU2012256033B2 (en) * | 2011-05-16 | 2015-07-02 | Colgate-Palmolive Company | Oral care composition for treating dry mouth |
| CN102940595A (en) * | 2012-12-04 | 2013-02-27 | 中国科学院新疆理化技术研究所 | Mouthwash with functions of antibiosis, antiphlogosis and deodorization, and preparation method and use thereof |
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