CN103728304A - Focusing method for pathological section scanner - Google Patents
Focusing method for pathological section scanner Download PDFInfo
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- CN103728304A CN103728304A CN201310704180.7A CN201310704180A CN103728304A CN 103728304 A CN103728304 A CN 103728304A CN 201310704180 A CN201310704180 A CN 201310704180A CN 103728304 A CN103728304 A CN 103728304A
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Abstract
The invention provides a focusing method for a pathological section scanner, and is applied to the focusing scanning of the pathological section scanner over section tissues. The method comprises the following steps that a lens reciprocally moves relative to the section tissues on a workbench, and obtains a focal length relative to a certain point on the section tissues during reciprocal movement; 2, the lens moves relative to the section tissues to obtain focal lengths relative to certain points on the section tissues while reciprocally moving. According to the technical scheme, the lens and the section tissues simultaneously and continuously move, and the focal lengths are sought for focusing during reciprocal movement, so that the focusing speed is increased to increase the whole pathological section scanning speed, and the quality of a scanned image is well improved.
Description
Technical field
The invention belongs to mechanical manufacturing field, relate to a kind of focusing method, relate in particular to a kind of focusing method for pathological section scanner.
Background technology
Pathological section is the one of Pathologic specimen.During making, part is had to the processing of tissue or the internal organs various chemicals of process and the burying storage of pathology, make it fixing sclerosis, on microtome, thinly slice, stick on slide, dye with shades of colour, for checking under the microscope, to observe pathological change, make pathological diagnosis, for clinical diagnosis and treatment are offered help.Pathological section scanner is that pathological section is changed into digitized a kind of instrument, substitutes microscope.
The focusing scanning technique that existing pathological section scanner is used is all in section, to select some points, then mobile camera moving is arrived to this point, calculates focus value, then calculates successively the focus value of other points, finally according to these focus value, scans one by one.Corresponding picture scanning out.Like this, if focus on manyly just greatly affected scanning bulk velocity, if focus on few picture quality that just affected.
Summary of the invention
In view of this, the present invention is by utilize camera lens and biopsy tissues to move and uninterruptedly simultaneously, in a lot of round movements, find focal length and focus, thereby not only improved focusing speed, improve pathological section scanning bulk velocity, and well improved scanning picture quality out.
For achieving the above object, concrete technical scheme is as follows:
A kind of focusing method for pathological section scanner, be applicable to the focusing scanning of pathologic section slicer to biopsy tissues, described pathologic section slicer comprises camera lens and worktable, and described biopsy tissues is placed on described worktable, for the focusing method of pathological section scanner, comprises the following steps:
Step 1, described camera lens moves back and forth with respect to the biopsy tissues on described worktable, and in camera lens described in described to-and-fro movement with respect to described biopsy tissues on certain any focal length;
Step 2, moved with respect to described biopsy tissues in the reciprocating while of described camera lens, obtained the focal length of the camera lens of some point on described biopsy tissues.
Preferably, the described camera lens in described step 1 does straight reciprocating motion with respect to described biopsy tissues.
Preferably, the described biopsy tissues in described step 2 is divided into several rows according to the sweep limit of described camera lens, and described biopsy tissues moves line by line with respect to described camera lens in described camera lens straight reciprocating motion.
Preferably, it is moving that the movement line by line of the described biopsy tissues in described step 2 comprises that X-axis moves with y-axis shift, and it is vertical that described X-axis moves the moving direction moving with y-axis shift.
When preferably, described biopsy tissues X-axis moves, at the uniform velocity move.
Preferably, the described camera lens in described step 1 is along Z axis straight reciprocating motion, and it is vertical that the direction that described camera lens Z axis moves and described biopsy tissues X-axis move the moving direction moving with y-axis shift.
When preferably, the described camera lens Z axis in described step 1 moves, at the uniform velocity move.
With respect to prior art, the advantage of technical scheme of the present invention has:
Technical scheme of the present invention is by utilize camera lens and biopsy tissues to move and uninterruptedly simultaneously, in a lot of round movements, finding focal length focuses, thereby not only improved focusing speed and improved pathological section scanning bulk velocity, and well improved scanning picture quality out.
Accompanying drawing explanation
The accompanying drawing that forms a part of the present invention is used to provide a further understanding of the present invention, and schematic description and description of the present invention is used for explaining the present invention, does not form inappropriate limitation of the present invention.In the accompanying drawings:
Fig. 1 is the structural representation of embodiments of the invention;
Fig. 2 is the schematic diagram of lens moving track in Fig. 1;
Fig. 3 is the schematic diagram of the pictures taken of embodiments of the invention camera lens.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Based on the embodiment in the present invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
It should be noted that, in the situation that not conflicting, the feature in embodiment and embodiment in the present invention can combine mutually.
Below with reference to accompanying drawing, embodiments of the invention are done to concrete explaination.
A kind of focusing method for pathological section scanner of embodiments of the invention as shown in Figure 1, is applicable to the focusing scanning of pathologic section slicer to biopsy tissues.
Pathologic section slicer comprises camera lens 1 and worktable, and biopsy tissues 2 is placed on worktable.As shown in Figures 1 and 2, for the focusing method of pathological section scanner, comprise the following steps:
Step 1, camera lens 1 moves back and forth with respect to the biopsy tissues 2 on worktable, and in to-and-fro movement camera lens 1 with respect to certain any focal length on biopsy tissues 2;
Step 2, moved with respect to biopsy tissues 2 in the reciprocating while of camera lens 1, obtained the focal length of the camera lens of some point on biopsy tissues 2.
Embodiments of the invention are by utilize camera lens and biopsy tissues to move and uninterruptedly simultaneously, in a lot of round movements, finding focal length focuses, thereby not only improved focusing speed and improved pathological section scanning bulk velocity, and well improved scanning picture quality out.
As shown in Figure 1, in an embodiment of the present invention, due to when making pathological section, the position that biopsy tissues 2 and section are put can not be level, so cut into slices under 20X or 40X, during scanning, focal length is different.If set up coordinate system, shown in Fig. 1, suppose that perpendicular to the direction of Fig. 1 be Y direction, the center line of camera lens 1 is Z-direction, biopsy tissues 2 is laterally X-direction.
And in conjunction with as shown in Figure 2, embodiments of the invention preferably include camera lens 2 along Z axis constantly up and down at the uniform velocity linear reciprocation move, in movement, camera lens 1 is less than or equal to this focal length with respect to camera lens 1 apart from the bee-line of any point on biopsy tissues 2.Then biopsy tissues 2 moves simultaneously line by line, and be preferably along X-axis and at the uniform velocity move, and first motionless along Y-axis, then move line by line.As shown in Figure 2, in the half period of moving at the Z axis of camera lens 1, always there is a correct focus value.In figure, broken line shown in 2 represents the movement locus of camera lens 1.Square point on broken line represents the focal length Z of Z axis when certain X value of section.
In an embodiment of the present invention, known camera lens 1, along the speed of Z axis and the top/bottom latitude that moves along Z axis, supposes that speed is Vz, and upper scope is Pzmin, and lower scope is Pzmax, and wherein Pzmax is the reference position of camera lens 1 along Z axis.Known biopsy tissues 2 is along the translational speed of X-axis, and reference position, supposes that speed is Vx, and reference position is Pxs.In Fig. 3 picture that to be camera lens 1 start to capture at T=0 wherein one.The frequency of known line camera is that a bar line is per second.Suppose that in Fig. 3, the lines 3 clear positions of presentation video are the b line capturing.Y-axis invariant position, is assumed to be py,
Precondition: the movement that guarantees camera lens 1 and biopsy tissues 2 starts simultaneously, is at the same time namely.
A, by the frequency of camera lens 1, and the camera lens 1 figure position clearly of grabbing.T=b/a；
B, according to T, calculates px(biopsy tissues 2 along the moving distance of y-axis shift)=T × Vx+Pxs;
C:1. how long the cycle that first calculates needs, t '=| Pzmin-Pzmax|/Vz;
2. according to T, calculating curve is at first transition, or last transition.And then calculate the remainder of T/t '.Suppose that remainder is c;
3. calculate pz=Pzmin-c × Vz or pz=Pzmax+c × Vz.
Finally calculating the focal length that (px, py) is corresponding is pz.
Above specific embodiments of the invention be have been described in detail, but it is as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that this practicality is carried out and alternative also all among category of the present invention.Therefore, equalization conversion and the modification done without departing from the spirit and scope of the invention, all should contain within the scope of the invention.
Claims (7)
1. for a focusing method for pathological section scanner, be applicable to the focusing scanning of pathologic section slicer to biopsy tissues, described pathologic section slicer comprises camera lens and worktable, and described biopsy tissues is placed on described worktable, it is characterized in that, comprises the following steps:
Step 1, described camera lens moves back and forth with respect to the biopsy tissues on described worktable, and in camera lens described in described to-and-fro movement with respect to described biopsy tissues on certain any focal length;
Step 2, moved with respect to described biopsy tissues in the reciprocating while of described camera lens, obtained the focal length of the camera lens of some point on described biopsy tissues.
2. the focusing method for pathological section scanner as claimed in claim 1, is characterized in that, the described camera lens in described step 1 does straight reciprocating motion with respect to described biopsy tissues.
3. the focusing method for pathological section scanner as claimed in claim 2, it is characterized in that, described biopsy tissues in described step 2 is divided into several rows according to the sweep limit of described camera lens, and described biopsy tissues moves line by line with respect to described camera lens in described camera lens straight reciprocating motion.
4. the focusing method for pathological section scanner as claimed in claim 3, is characterized in that, it is moving that the movement line by line of the described biopsy tissues in described step 2 comprises that X-axis moves with y-axis shift, and it is vertical that described X-axis moves the moving direction moving with y-axis shift.
5. the focusing method for pathological section scanner as claimed in claim 4, is characterized in that, described biopsy tissues X-axis at the uniform velocity moves while moving.
6. the focusing method for pathological section scanner as claimed in claim 5, it is characterized in that, described camera lens in described step 1 is along Z axis straight reciprocating motion, and it is vertical that the direction that described camera lens Z axis moves and described biopsy tissues X-axis move the moving direction moving with y-axis shift.
7. the focusing method for pathological section scanner as claimed in claim 6, is characterized in that, the described camera lens Z axis in described step 1 at the uniform velocity moves while moving.
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| CN201310704180.7A CN103728304A (en) | 2013-12-18 | 2013-12-18 | Focusing method for pathological section scanner |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104390981A (en) * | 2014-12-12 | 2015-03-04 | 宁波江丰生物信息技术有限公司 | Method for rapidly and accurately focusing and scanning pathological section tissue on basis of image collection device |
| CN107561075A (en) * | 2017-07-26 | 2018-01-09 | 山东颐泽天泰医疗科技有限公司 | The fast scan imaging method of cell DNA ploidy analysis three-dimensional movement platform |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1229931A (en) * | 1998-03-25 | 1999-09-29 | 全友电脑股份有限公司 | Automatic focusing method |
| JP2001059935A (en) * | 1999-06-14 | 2001-03-06 | Olympus Optical Co Ltd | Automatic focus detector, automatic focus detecting method and automatic focus detecting program-recorded recording medium |
| JP2004191846A (en) * | 2002-12-13 | 2004-07-08 | Olympus Corp | Laser scanning type microscope |
| WO2004095360A1 (en) * | 2003-04-17 | 2004-11-04 | Aperio Technologies | Method and apparatus for pre-focus in a linear array based slide scanner |
| CN1967245A (en) * | 2005-09-30 | 2007-05-23 | 希森美康株式会社 | Microscope and auto focusing method for a microscope |
| CN101603821A (en) * | 2008-06-13 | 2009-12-16 | 鸿富锦精密工业（深圳）有限公司 | The focusing method of image measurer and image measurer |
| CN102427502A (en) * | 2011-10-01 | 2012-04-25 | 麦克奥迪实业集团有限公司 | Scanning method and device for microscopic section |
| CN102566023A (en) * | 2012-01-11 | 2012-07-11 | 麦克奥迪实业集团有限公司 | Digital slide real time scanning automatic focusing system and method thereof |
| CN102998787A (en) * | 2012-07-24 | 2013-03-27 | 中华人民共和国南京出入境检验检疫局 | Method for acquiring digital focused images of fiber cross section slices under microscope |
| CN103163638A (en) * | 2011-12-09 | 2013-06-19 | 姚伯元 | Microscope automatic focusing method based on sample determination surface plane equation |
| CN103189779A (en) * | 2010-10-22 | 2013-07-03 | 威廉·吉尔博特 | Automated slide scanning system for a microscope |
-
2013
- 2013-12-18 CN CN201310704180.7A patent/CN103728304A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1229931A (en) * | 1998-03-25 | 1999-09-29 | 全友电脑股份有限公司 | Automatic focusing method |
| JP2001059935A (en) * | 1999-06-14 | 2001-03-06 | Olympus Optical Co Ltd | Automatic focus detector, automatic focus detecting method and automatic focus detecting program-recorded recording medium |
| JP2004191846A (en) * | 2002-12-13 | 2004-07-08 | Olympus Corp | Laser scanning type microscope |
| WO2004095360A1 (en) * | 2003-04-17 | 2004-11-04 | Aperio Technologies | Method and apparatus for pre-focus in a linear array based slide scanner |
| CN1967245A (en) * | 2005-09-30 | 2007-05-23 | 希森美康株式会社 | Microscope and auto focusing method for a microscope |
| CN101603821A (en) * | 2008-06-13 | 2009-12-16 | 鸿富锦精密工业（深圳）有限公司 | The focusing method of image measurer and image measurer |
| CN103189779A (en) * | 2010-10-22 | 2013-07-03 | 威廉·吉尔博特 | Automated slide scanning system for a microscope |
| US20130222895A1 (en) * | 2010-10-22 | 2013-08-29 | William Gelbart | Automated slide scanning system for a microscope |
| CN102427502A (en) * | 2011-10-01 | 2012-04-25 | 麦克奥迪实业集团有限公司 | Scanning method and device for microscopic section |
| CN103163638A (en) * | 2011-12-09 | 2013-06-19 | 姚伯元 | Microscope automatic focusing method based on sample determination surface plane equation |
| CN102566023A (en) * | 2012-01-11 | 2012-07-11 | 麦克奥迪实业集团有限公司 | Digital slide real time scanning automatic focusing system and method thereof |
| CN102998787A (en) * | 2012-07-24 | 2013-03-27 | 中华人民共和国南京出入境检验检疫局 | Method for acquiring digital focused images of fiber cross section slices under microscope |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104390981A (en) * | 2014-12-12 | 2015-03-04 | 宁波江丰生物信息技术有限公司 | Method for rapidly and accurately focusing and scanning pathological section tissue on basis of image collection device |
| CN107561075A (en) * | 2017-07-26 | 2018-01-09 | 山东颐泽天泰医疗科技有限公司 | The fast scan imaging method of cell DNA ploidy analysis three-dimensional movement platform |
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Application publication date: 20140416 |