CN103609908B - Immune preparation for improving immune function of grass carp - Google Patents
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- CN103609908B CN103609908B CN201310630290.3A CN201310630290A CN103609908B CN 103609908 B CN103609908 B CN 103609908B CN 201310630290 A CN201310630290 A CN 201310630290A CN 103609908 B CN103609908 B CN 103609908B
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Abstract
The invention provides a traditional Chinese medicine immune preparation for improving the immune function of grass carp. The traditional Chinese medicine immune preparation comprises the following raw materials of grifola, acanthopanax, atractylodes macrocephala koidz, ginseng leaves, cordate houttuynia, eucommia ulmoides, eclipta, rhizoma drynariae, cassia twig, isatis root, hawthorn, endothelium corneum gigeriae galli, campsis grandiflora, Chinese yam, caulis spatholobi, spina gleditsiae, dogwood, peach juvenile branchlet, fructus cnidii, Chinese-date, radix stemonae, semen euryales, fructus galangae, areca-nut, pittosporm root and horseweed herb. The traditional Chinese medicine immune preparation provided by the invention has lots of advantages such as high efficiency, low toxic and side effects, high safety and low residual, and is wide in source and low in cost; the traditional Chinese medicine immune preparation is capable of adjusting the immune system of fish, improving the anti-stress and immune functions of aquatic livestock, thus preventing and treating fish diseases.
Description
Technical field
The present invention relates to technical field of Chinese medicine, particularly relating to a kind of feed for improving grass carp immunity function.
Background technology
Along with the development of aquatic products intensive degree, aquatic animal disease progressively presents the feature of multiple, complicated, high harm, and the deterioration of cultivation water environment also causes fish immunity power to decline.For reply fish disease spreading trend, a large amount of splashing of fish insect killing medicine is used, abuse and the external sterilization medicine of Multiple Classes of Antibiotics are splashed, although have certain control action to disease, but often cause the drug resistance of pathogenic microorganisms, medicament residue, even cause whole water ecological setting to balance and destroy.
In recent years, grass carp (
ctenopha ryngodon idellus) because its efficiency of feed utilization is high, aquaculture cost is cheap, it is rapid, best in quality to grow, deeply like by broad masses, developed into the govern-house-variety of China's freshwater aquiculture.Grass carp is one of China " four large Chinese carps ", but due to the incidence of disease (average about 50 % of grass carp'disease, sometimes up to 90 more than %), case fatality rate (generally at 90 more than %) is very high, popular very wide, fish production is made a big impact.Prevent and treat problem in order to what solve grass carp'disease, need the immunity function conscientiously improving grass carp.
Chinese herbal medicine is a kind of desirable natural, environment-friendly medicine; have efficient, toxic and side effect is little, security is high, the residual plurality of advantages such as few; and wide material sources; cost is low, adjusts the immune system of fish, improves the dynamic anti-stress ability of aquatic products and immunity function; preventing and curing of fish disease; Chinese herbal medicine, as disease prevention and cure medicine or additive of bait, is particularly useful for the needs of intensive, the large-scale production of current culture fishery, is convenient to the colony's control carrying out cultured fishes disease.And application herbal control fish disease meets the nuisanceless aquatic products industry of development completely, produces the disease control criterion of green aquatic product.By the immunity using raising aquatic livestock of Chinese herbal medicine, improve animal disease resistant power, the incidence of disease reducing aquatic livestock is the focus studied in recent years.Advocating healthy aquaculture under the new situation at present comprehensively, promoting herbal control fish disease, be extremely important.
The present invention is exactly on this basis, a kind of Traditional Chinese medicine immune preparation for improving grass carp immunity function is proposed, it has efficiently, toxic and side effect is little, security is high, remain the plurality of advantages such as few, and wide material sources, cost is low, the immune system of fish is adjusted, improves the dynamic anti-stress ability of aquatic products and immunity function, thus preventing and curing of fish disease.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Traditional Chinese medicine immune preparation for improving grass carp immunity function, it has efficiently, toxic and side effect is little, security is high, remain the plurality of advantages such as few, and wide material sources, cost is low, the immune system of fish is adjusted, improve the dynamic anti-stress ability of aquatic products and immunity function, thus preventing and curing of fish disease.
For solving the problems of the technologies described above, the invention provides a kind of Traditional Chinese medicine immune preparation for improving grass carp immunity function, its bulk drug comprises umbellate pore furgus, wilsonii, the bighead atractylodes rhizome, folium panacis japonici cum caule, cordate houttuynia, the bark of eucommia, eclipta, the rhizome of davallia, cassia twig, Radix Isatidis, hawthorn, the membrane of a chicken's gizzard, Chinese trumpet creeper, Chinese yam, reticulate millettia, spina gleditsiae, Fructus Corni, peach branch, frutus cnidii, date, the tuber of stemona, Gorgon fruit, Galanga Galangal Seed, betel nut, pittosporum illicioides and Horseweed Herb.
Wherein, in described Traditional Chinese medicine immune preparation, the weight of each bulk drug is respectively umbellate pore furgus 25g ~ 35g, wilsonii 45g ~ 55g, bighead atractylodes rhizome 35g ~ 45g, folium panacis japonici cum caule 60g ~ 70g, cordate houttuynia 25g ~ 35g, bark of eucommia 30g ~ 40g, eclipta 35g ~ 45g, rhizome of davallia 30g ~ 40g, cassia twig 25g ~ 35g, Radix Isatidis 35g ~ 45g, hawthorn 35g ~ 45g, the membrane of a chicken's gizzard 25g ~ 35g, Chinese trumpet creeper 20g ~ 30g, Chinese yam 25g ~ 35g, reticulate millettia 35g ~ 45g, spina gleditsiae 25g ~ 35g, Fructus Corni 30g ~ 40g, peach branch 25g ~ 35g, frutus cnidii 35g ~ 45g, date 30g ~ 40g, tuber of stemona 20g ~ 30g, Gorgon fruit 25g ~ 35g, Galanga Galangal Seed 20g ~ 30g, betel nut 25g ~ 35g, pittosporum illicioides 35g ~ 45g and Horseweed Herb 30g ~ 40g.
Wherein, in described Traditional Chinese medicine immune preparation, the weight of each bulk drug is respectively umbellate pore furgus 30g, wilsonii 50g, bighead atractylodes rhizome 40g, folium panacis japonici cum caule 65g, cordate houttuynia 30g, bark of eucommia 35g, eclipta 40g, rhizome of davallia 35g, cassia twig 30g, Radix Isatidis 40g, hawthorn 40g, the membrane of a chicken's gizzard 30g, Chinese trumpet creeper 25g, Chinese yam 30g, reticulate millettia 40g, spina gleditsiae 30g, Fructus Corni 35g, peach branch 30g, frutus cnidii 40g, date 35g, tuber of stemona 25g, Gorgon fruit 30g, Galanga Galangal Seed 25g, betel nut 30g, pittosporum illicioides 40g and Horseweed Herb 35g.
Present invention also offers the preparation method of above-mentioned Traditional Chinese medicine immune preparation, be specially:
Each bulk drug in Traditional Chinese medicine immune preparation is uniformly mixed, send in pulverizer and be crushed and screened into 20 order ~ 40 object powder, put into marmite subsequently, add the water of 4 ~ 6 times of quality, soak 2 ~ 4 hours, then 1 ~ 2 hour is decocted, concoction is poured out, again add the water relative to powder quality 2 ~ 4 times, decoct 30 minutes ~ 1 hour, filter, the concoction obtained and the concoction obtained above mixing will be filtered, the water of reduced pressure concentration removing 70% ~ 80% volume, add ethyl acetate subsequently to extract, the ethyl acetate solution of acquisition is carried out reduced pressure concentration removing ethyl acetate solvent wherein, dry subsequently, dry body is sent in pulverizer again and pulverizes and be screened into 20 order ~ 40 object powder, with the starch relative to its quality 3 ~ 5 times, mixing, granulate with 14 mesh sieves, toast 30 minutes in 60 DEG C ~ 70 DEG C baking ovens, obtain Traditional Chinese medicine immune preparation.
Present invention also offers a kind of feed for improving grass carp immunologic function, it comprises rape oil, dregs of beans, fish meal, salt, wheat flour, Traditional Chinese medicine immune preparation, bacillus subtilis, mineral element additive, vitamine supplement and calcium dihydrogen phosphate.
Wherein, in described feed, the weight of each raw material is respectively rape oil 50g ~ 70g, dregs of beans 900g ~ 1100g, fish meal 50g ~ 70g, salt 10g ~ 20g, wheat flour 200g ~ 300g, Traditional Chinese medicine immune preparation 100g ~ 150g, bacillus subtilis 3g ~ 5g, mineral element additive 10g ~ 20g, vitamine supplement 20g ~ 30g, calcium dihydrogen phosphate 5g ~ 10g.
Wherein, in described feed, the weight of each raw material is respectively rape oil 60g, dregs of beans 1000g, fish meal 60g, salt 15g, wheat flour 250g, Traditional Chinese medicine immune preparation 130g, bacillus subtilis 4g, mineral element additive 15g, vitamine supplement 25g, calcium dihydrogen phosphate 8g.
The preparation method of the above-mentioned feed that the present invention also provides, is specially:
The first step, each bulk drug in Traditional Chinese medicine immune preparation is uniformly mixed, send in pulverizer and be crushed and screened into 20 order ~ 40 object powder, put into marmite subsequently, add the water of 4 ~ 6 times of quality, soak 2 ~ 4 hours, then 1 ~ 2 hour is decocted, concoction is poured out, again add the water relative to powder quality 2 ~ 4 times, decoct 30 minutes ~ 1 hour, filter, the concoction obtained and the concoction obtained above mixing will be filtered, the water of reduced pressure concentration removing 70% ~ 80% volume, add ethyl acetate subsequently to extract, the ethyl acetate solution of acquisition is carried out reduced pressure concentration removing ethyl acetate solvent wherein, dry subsequently, dry body is sent in pulverizer again and pulverizes and be screened into 20 order ~ 40 object powder, with the starch relative to its quality 3 ~ 5 times, mixing, granulate with 14 mesh sieves, toast 30 minutes in 60 DEG C ~ 70 DEG C baking ovens, obtain Traditional Chinese medicine immune preparation,
Second step, other each components in the Chinese medicine composition of first step acquisition and described feed are sent into stirrer for mixing in proportion even, mixture homogeneity is CV%≤10%;
3rd step, the material mixed is modulated, and temperature controls at 90 ~ 95 DEG C, and modulating time 55 ~ 65 seconds, granulates subsequently, cooling, and pellet composition cools, and temperature controls at 20 ~ 25 DEG C, packaging.
Beneficial effect of the present invention:
Traditional Chinese medicine immune preparation provided by the invention has efficiently, toxic and side effect is little, security is high, remain the plurality of advantages such as few, and wide material sources, cost is low, adjusts the immune system of fish, improve the dynamic anti-stress ability of aquatic products and immunity function, thus preventing and curing of fish disease.
Detailed description of the invention
The invention provides a kind of Traditional Chinese medicine immune preparation for improving grass carp immunity function, its bulk drug comprises umbellate pore furgus, wilsonii, the bighead atractylodes rhizome, folium panacis japonici cum caule, cordate houttuynia, the bark of eucommia, eclipta, the rhizome of davallia, cassia twig, Radix Isatidis, hawthorn, the membrane of a chicken's gizzard, Chinese trumpet creeper, Chinese yam, reticulate millettia, spina gleditsiae, Fructus Corni, peach branch, frutus cnidii, date, the tuber of stemona, Gorgon fruit, Galanga Galangal Seed, betel nut, pittosporum illicioides and Horseweed Herb.
Further, described Traditional Chinese medicine immune preparation is only made up of above-mentioned raw materials medicine.
The pharmacology of each bulk drug is as follows:
Umbellate pore furgus: taste is sweet, light, property is put down, and enters kidney, urinary bladder channel, has the effect of clearing damp and promoting diuresis, be mainly used in the oedema of retention of water-damp, difficult urination, and the having loose bowels of water retention due to hypofunction of the spleen, vomits; The card such as leukorrhea, gonorrhoea of damp invasion of lower energizer.
Wilsonii: latin name
acanthopanacis Senticosi Radix et Rhizomaseu Caulis, taste is pungent, bitter, micro-sweet, warm in nature, enter spleen, kidney, the heart channel of Hang-Shaoyin, have and replenish qi to invigorate the spleen, tonify the kidney to relieve mental strain, the effect of dispelling rheumatism, soft for wind-cold-dampness arthralgia, lumbocrural pain, muscles and bones impotence, be slow in action, the card such as body void is thin and weak, traumatic injury, fracture, oedema, beriberi, uncontrolled urination are itched.
The bighead atractylodes rhizome: latin name
attractylodis Macrocephalae Rhizoma, taste is sweet, bitter, warm in nature, enters spleen, stomach warp, has tonifying spleen profit gas, eliminating dampness Li Shui, strengthening exterior and reducing sweat, the fortune spleen effect of moisturizing, and cures mainly the caused ruffian of the dysfunction of the spleen in transport, QI failing to transform into body fluid and has one's mind filled with and rush down, phlegm and retained fluid oedema; The vexed lazyness speech of the deficiency of qi in middle-jiao, metrorrhagia and metrostaxis, proctoptosis with chronic dysentery; The cards such as solid in the playing of deficiency of the spleen causing weakness of QI, abnormal sweating spontaneous perspiration, the bighead atractylodes rhizome can promote gastric secretion, strengthens the absorption of small intestine, has regulating action to gastrointestinal function.
Folium panacis japonici cum caule: bitter, sweet, cold in nature, attach to the lung and stomach meridians, have tonifying Qi, beneficial lung, drives away summer heat, and the effect of promoting the production of body fluid, cures mainly qi-asthenia cough, and hot summer weather is irritated, and Tianjin wound is thirsty, and the head is unclear, and four limbs are tired wearyly waits card.
Cordate houttuynia: taste is pungent, cold nature, enters lung, urinary bladder channel, has clearing heat and detoxicating, removing pus and relieving carbuncle, effect of inducing diuresis for treating strangurtia, and for lung carbuncle pyemesis, phlegm heat is coughed and breathed heavily, toxic-heat and blood stasis, damp-heat gonorrhoea, hot carbuncle carbuncle, and snakeworm wants first-class card.
The bark of eucommia: taste is sweet, micro-pungent, warm in nature, enters liver, kidney channel, has hypotensive, filling liver kidney, strengthening the bones and muscles, the effect of antiabortive gas, for caused by liver and kidney deficiency, card see dizzy, soreness of waist and knee joint, muscles and bones impotence are weak; The unconsolidation of the kidney-qi, frequent micturition or dripping urination, uncontrolled urination are itched, impotence, pregnant woman are weak, fetal irritability or waist pendant pain; The cards such as chronic joint diseases, bone tuberculosis, dysmenorrhoea, dysfunctional uterine bleeding, chronic pelvic inflammatory disease.
Eclipta: taste is sweet, sour, cold in nature, returns liver, kidney channel, plays and tonify the liver and kidney, the effect of cooling blood and hemostasis, for having a dizzy spell caused by the deficiency of liver-yin and kidney-yin, blurring of vision, and soreness and weakness of waist and knees; Be suitable for the deficiency of Yin and have hot person, be used for the treatment of the haematemesis of accumulated heat in stomach, the cards such as the hemoptysis of heat impairing pulmonary collateral.
The rhizome of davallia: bitter, warm in nature, enter liver, kidney channel, there is nourishing disney and strengthening bone, continuous effect of hindering pain relieving, for soreness of waist and knee joint of suffering from a deficiency of the kidney, flaccidity and weakness of the lower limbs, the deficiency of liver-yin and kidney-yin, empty positive rising and the deafness and tinnitus that causes, tooth mobility pain or bleeding gums, speen-kidney dificiency and cause and rush down the cards such as protracted dysentery for a long time.
Cassia twig: taste is pungent, sweet, warm in nature, the thoughts of returning home, lung, urinary bladder channel, there is sweating expelling pathogenic factors from muscles and skin, warming meridians and promoting circulation of qi, help yang transforming qi, the effect of eliminating cold to stop pain, cassia twig warming meridians and promoting circulation of qi, horizontal logical podomere, for illness that has not attacked the vital organs of the human body of catching cold, no matter have sweat, losslessly all can to apply, also for arthralgia due to wind-dampness, stomach cold stomachache, through closing, dysmenorrhoea, arthralgia due to wind-dampness, the cards such as shoulder arm podomere crymodynia.
Radix Isatidis: latin name
isatidis Radix,bitter, cold in nature, enter the heart, liver, courage, stomach warp, have the effect of clearing heat and detoxicating, cool blood relieve sore throat, be used for the treatment of febrile virulent maculae; High heat headache; Major part pestilence; Tongue is secretly dark reddish purple, scarlet fever; Erysipelas; Mumps; Larynx numbness; Sore is swollen, carbuncle swells; Varicella; Measles; Hepatitis; Influenza, epidemic meningitis, encephalitis, pneumonia, coma tells nosebleed, and pharynx is swollen, pinkeye, sore rash; The cards such as Japanese Type-B encephalitis, acute, chronic hepatitis, mumps, osteomyelitis can be prevented and treated.
Hawthorn: taste acid, sweet, slightly warm in nature, returns spleen, stomach, Liver Channel, and for food digesting stomach fortifying, promoting the circulation of qi is fallen apart the stasis of blood, and be mainly used to treat accumulation of food in the stomach and intes tine due to indigestion, have loose bowels, dysentery, hawthorn be the staple for the treatment of indigestion, and hawthorn can increase the secretion of peptic digest enzyme, promotes to digest; To shigella dysenteriae, Escherichia coli, Pseudomonas aeruginosa, staphylococcus aureus etc., there is obvious inhibitory action, the effect that the active ingredient hawthorn fruit total flavone in hawthorn and hawthorn total triterpene acids have good reducing blood lipid, reduce cholesterol, increase Gastric juice digestion enzyme.
The membrane of a chicken's gizzard: taste is sweet, cold in nature, return spleen, stomach, small intestine, urinary bladder channel, there is food digesting stomach fortifying, effect of arresting seminal emission, cure mainly dyspepsia turgor, vomiting gastric disorder causing nausea, rushes down dysentery, infantile malnutrition due to digestive disturbances or intestinalparasites, quench one's thirst, lose and drown, larynx numbness acute tonsillitis, the cards such as noma aphtha, the membrane of a chicken's gizzard has promotion gastric secretion, effect of gastric acidity and enteral digestion absorbability.
Chinese trumpet creeper: latin name
campsis Flos, taste is sweet, sour, cold in nature, returns liver, pericardium channel, has promoting circulation of blood and goes the stasis of blood, effect of cooling blood, expelling wind, and for Jing Bi wei lump in the abdomen, postpartum, breast was swollen, and rubella is rubescent, pruitus, the cards such as acne.
Chinese yam: taste is sweet, property is put down, enter lung, spleen, kidney channel, have strengthening the spleen and tonifying the lung, beneficial stomach kidney tonifying, benefit of reinforcing the kidney essence, hearing-improing and eyesight improving, effect of helping the five internal organs, strengthening the bones and muscles, long will to calm the nerves, promote longevity, cure mainly weakness of the spleen and the stomach, lassitude, poor appetite, chronic diarrhea and dysentery, Lung Qi deficiency are dry, asthma due to excessive phlegm cough, kidney qi loss, soreness and weakness of waist and knees, lower limb impotence are weak, frequent micturition of quenching one's thirst, seminal emission premature ejaculation, band descend the red card such as swollen, fat of gonorrhoea, skin.
Reticulate millettia: bitter, sweet, warm in nature, return liver, kidney channel, have hemangiectasis, enrich blood and invigorate blood circulation, menstruation regulating, the effect of stimulating the circulation of the blood and cause the muscles and joints to relax, cure mainly the irregular menstruation that the deficiency of blood has silt, limbs pain is meciless, the card such as soreness and weakness of waist and knees, pain.
Spina gleditsiae: taste is pungent, warm in nature, return lung channel, cure mainly swollen ulcer drug, scrofula, sore rash stubborn dermatitis, hypogalactia after delivery, retention of afterbirth, the cards such as pestilence wind.
Fructus Corni: taste is sour, puckery, and slightly warm in nature, returns liver, kidney channel, have and tonify the liver and kidney, effect that puckery essence takes off admittedly.For dizzy tinnitus, soreness of waist and knee joint, impotence and seminal emission, enuresis frequent micturition, under uterine bleeding band, profuse sweating is collapsed.Interior heat such as to be quenched one's thirst at the card.
Peach branch: latin name
persicae Ramulus, bitter, property is put down, and the thoughts of returning home, Liver Channel, have activating blood to promote menstruation, effect of removing toxic substances desinsection, for trusted subordinate's shouting pain, arthralgia due to wind-dampness, the cards such as traumatic injury.
Frutus cnidii: taste is pungent, bitter, warm in nature, enters kidney channel, has warming kidney to invigorate yang, removing dampness and destroying parasites, the effect of dispellingging wind and arresting itching, and the impotence in male caused for decline of kidney-YANG, woman Gong Leng are infertile, under cold-dampness band or the card such as waist knee crymodynia.
Date: taste is sweet, warm in nature, enter spleen, stomach warp, there is the effect of air making-up and spleen enlivening, nourishing blood and tranquilization, harmonizing yingfen and weifen, the mitigation property of medicine, for weakness of the spleen and the stomach, fatigue and asthenia, anorexia and loose stool, the cards such as insufficiency of vital energy and blood, has a headache and fever, sweating hot blast.
The tuber of stemona: taste is sweet, bitter, slightly warm in nature enters lung channel, have and moisten the lung and relieve the cough, the effect of delousing desinsection, tuber of stemona temperature embellishes but not dry, and property is kept and do not walk, there is out again the merit of drop-down gas, be mainly used in newly all for a long time coughing, be especially suitable for the hot chronic cough person of labor, the modern times are used for the cough preventing and treating chronic bronchitis, the dry cough of pulmonary tuberculosis, the cards such as pertussis.
Gorgon fruit: taste is sweet, puckery, property is put down.Return spleen, kidney channel.There is supplementing the kidney to control the nocturnal, tonifying spleen antidiarrheal, the function that clearing damp is only with, cure mainly emission, splenasthenic diarrhea, insufficiency of the spleen gonorrhoea, be with inferior card.
Galanga Galangal Seed: latin name
hedysari Radix, taste is pungent, warm in nature, returns spleen, lung channel, has eliminating dampness in temperature, and eliminating dampness is loose cold, effect helped digestion of being amusing, and cures mainly coldness and pain in the epigastrium, the card such as dyspepsia turgor, vomiting are had loose bowels, drinking too much.
Betel nut: bitter, pungent, warm in nature, returns stomach, large intestine channel, has desinsection, disappear long-pending, promoting the circulation of qi, Li Shui, effect of preventing malaria, cures mainly abdominal pain due to enterositosis, stagnates and rushes down dysentery, tenesmus, oedema beriberi, the cards such as malaria, betel nut can promote that stomach moves, and improves the secretion of motilin and vasoactive intestinal peptide.
Pittosporum illicioides: bitter, pungent, warm in nature, have activating collaterals to relieve pain, heart-calming kidney-nourishing, effect of removing toxic substances, cures mainly arthralgia due to wind-dampness, fracture, stomachache, insomnia, seminal emission, the cards such as venomous snake bite.
Horseweed Herb: mildly bitter flavor, pungent, cool in nature, have clearing heat and promoting diuresis, effect of removing toxicity for detumescence, cures mainly dysentery, enteritis, hepatitis, cholecystitis, tympanitis, conjunctivitis, traumatic injury, treating rheumatic ostealgia, traumatism and bleeding, the cards such as eczema.
Wherein, in described Traditional Chinese medicine immune preparation, the weight of each bulk drug is respectively umbellate pore furgus 25g ~ 35g, wilsonii 45g ~ 55g, bighead atractylodes rhizome 35g ~ 45g, folium panacis japonici cum caule 60g ~ 70g, cordate houttuynia 25g ~ 35g, bark of eucommia 30g ~ 40g, eclipta 35g ~ 45g, rhizome of davallia 30g ~ 40g, cassia twig 25g ~ 35g, Radix Isatidis 35g ~ 45g, hawthorn 35g ~ 45g, the membrane of a chicken's gizzard 25g ~ 35g, Chinese trumpet creeper 20g ~ 30g, Chinese yam 25g ~ 35g, reticulate millettia 35g ~ 45g, spina gleditsiae 25g ~ 35g, Fructus Corni 30g ~ 40g, peach branch 25g ~ 35g, frutus cnidii 35g ~ 45g, date 30g ~ 40g, tuber of stemona 20g ~ 30g, Gorgon fruit 25g ~ 35g, Galanga Galangal Seed 20g ~ 30g, betel nut 25g ~ 35g, pittosporum illicioides 35g ~ 45g and Horseweed Herb 30g ~ 40g.
Wherein, in described Traditional Chinese medicine immune preparation, the weight of each bulk drug is respectively umbellate pore furgus 30g, wilsonii 50g, bighead atractylodes rhizome 40g, folium panacis japonici cum caule 65g, cordate houttuynia 30g, bark of eucommia 35g, eclipta 40g, rhizome of davallia 35g, cassia twig 30g, Radix Isatidis 40g, hawthorn 40g, the membrane of a chicken's gizzard 30g, Chinese trumpet creeper 25g, Chinese yam 30g, reticulate millettia 40g, spina gleditsiae 30g, Fructus Corni 35g, peach branch 30g, frutus cnidii 40g, date 35g, tuber of stemona 25g, Gorgon fruit 30g, Galanga Galangal Seed 25g, betel nut 30g, pittosporum illicioides 40g and Horseweed Herb 35g.
Present invention also offers the preparation method of above-mentioned Traditional Chinese medicine immune preparation, be specially:
Each bulk drug in Traditional Chinese medicine immune preparation is uniformly mixed, send in pulverizer and be crushed and screened into 20 order ~ 40 object powder, put into marmite subsequently, add the water of 4 ~ 6 times of quality, soak 2 ~ 4 hours, then 1 ~ 2 hour is decocted, concoction is poured out, again add the water relative to powder quality 2 ~ 4 times, decoct 30 minutes ~ 1 hour, filter, the concoction obtained and the concoction obtained above mixing will be filtered, the water of reduced pressure concentration removing 70% ~ 80% volume, add ethyl acetate subsequently to extract, the ethyl acetate solution of acquisition is carried out reduced pressure concentration removing ethyl acetate solvent wherein, dry subsequently, dry body is sent in pulverizer again and pulverizes and be screened into 20 order ~ 40 object powder, with the starch relative to its quality 3 ~ 5 times, mixing, granulate with 14 mesh sieves, toast 30 minutes in 60 DEG C ~ 70 DEG C baking ovens, obtain Traditional Chinese medicine immune preparation.
Present invention also offers a kind of feed for improving grass carp immunologic function, it comprises rape oil, dregs of beans, fish meal, salt, wheat flour, above-mentioned Traditional Chinese medicine immune preparation, bacillus subtilis, mineral element additive, vitamine supplement and calcium dihydrogen phosphate.
Further, described feed is only made up of above-mentioned raw materials.
Wherein, in described feed, the weight of each raw material is respectively rape oil 50g ~ 70g, dregs of beans 900g ~ 1100g, fish meal 50g ~ 70g, salt 10g ~ 20g, wheat flour 200g ~ 300g, Traditional Chinese medicine immune preparation 100g ~ 150g, bacillus subtilis 3g ~ 5g, mineral element additive 10g ~ 20g, vitamine supplement 20g ~ 30g, calcium dihydrogen phosphate 5g ~ 10g.
Wherein, in described feed, the weight of each raw material is respectively rape oil 60g, dregs of beans 1000g, fish meal 60g, salt 15g, wheat flour 250g, Traditional Chinese medicine immune preparation 130g, bacillus subtilis 4g, mineral element additive 15g, vitamine supplement 25g, calcium dihydrogen phosphate 8g.
Described vitamine supplement can be various vitamin, as vitamin A, vitamin C, vitamin E etc., preferred vitamin premix, described vitamin premix can be FV-102 vitamin compound premix (Zhongmu Industry Co., Ltd's production), and it contains, and vitamin C is 100g/kg, vitamin E is 50,000 IU/kg.
The immunity function of mineral element to aquatic livestock has a very important role, it is by acting on the immune organ of aquatic livestock and affecting its immunologic function and health status with factors such as Ia enzymes, when the mineral element in feed and water body lacks or is excessive, the immunologic function of capital infringement aquatic livestock, increases its neurological susceptibility to disease.Therefore, in aquatic livestock, the appropriate mineral element that uses can play growth promoting effects, improve the effect of immunity and resistance against diseases, mineral substance premix provided by the present invention can be containing zinc, iron, copper, the compound of the trace element such as manganese or the mixture of amino-acid chelate, also the existing mineral substance premix additive that can buy on the market can be selected, as the micro-composite trace element premix additive of excellent profit that Beijing You Libao Bioisystech Co., Ltd produces, its main component is ferrous sulfate, Copper lysinate, zinc methionine, manganese sulfate, sodium selenite, Potassiumiodate, cobalt chloride etc., carrier is medical stone, wherein in every kg additive, iron content is 30g, copper content 17g, Zn content 30g, Fe content 20g.
The preparation method of the above-mentioned feed that the present invention also provides, is specially:
The first step, each bulk drug in Traditional Chinese medicine immune preparation is uniformly mixed, send in pulverizer and be crushed and screened into 20 order ~ 40 object powder, put into marmite subsequently, add the water of 4 ~ 6 times of quality, soak 2 ~ 4 hours, then 1 ~ 2 hour is decocted, concoction is poured out, again add the water relative to powder quality 2 ~ 4 times, decoct 30 minutes ~ 1 hour, filter, the concoction obtained and the concoction obtained above mixing will be filtered, the water of reduced pressure concentration removing 70% ~ 80% volume, add ethyl acetate subsequently to extract, the ethyl acetate solution of acquisition is carried out reduced pressure concentration removing ethyl acetate solvent wherein, dry subsequently, dry body is sent in pulverizer again and pulverizes and be screened into 20 order ~ 40 object powder, with the starch relative to its quality 3 ~ 5 times, mixing, granulate with 14 mesh sieves, toast 30 minutes in 60 DEG C ~ 70 DEG C baking ovens, obtain Traditional Chinese medicine immune preparation,
Second step, other each components in the Chinese medicine composition of first step acquisition and described feed are sent into stirrer for mixing in proportion even, mixture homogeneity is CV%≤10%;
3rd step, the material mixed is modulated, and temperature controls at 90 ~ 95 DEG C, and modulating time 55 ~ 65 seconds, granulates subsequently, cooling, and pellet composition cools, and temperature controls at 20 ~ 25 DEG C, packaging.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.
The preparation of embodiment 1 Traditional Chinese medicine immune preparation 1
By umbellate pore furgus 30g, wilsonii 50g, bighead atractylodes rhizome 40g, folium panacis japonici cum caule 65g, cordate houttuynia 30g, bark of eucommia 35g, eclipta 40g, rhizome of davallia 35g, cassia twig 30g, Radix Isatidis 40g, hawthorn 40g, the membrane of a chicken's gizzard 30g, Chinese trumpet creeper 25g, Chinese yam 30g, reticulate millettia 40g, spina gleditsiae 30g, Fructus Corni 35g, peach branch 30g, frutus cnidii 40g, date 35g, tuber of stemona 25g, Gorgon fruit 30g, Galanga Galangal Seed 25g, betel nut 30g, pittosporum illicioides 40g and Horseweed Herb 35g is uniformly mixed, send in pulverizer and be crushed and screened into 40 object powder, put into marmite subsequently, add the water of 4.5kg, soak 3 hours, then decoct 2 hours, concoction is poured out, again add the water of 2.5kg, decoct 1 hour, filter, will the concoction obtained and the concoction obtained mixing be filtered above, reduced pressure concentration removes the water of 80% volume, add ethyl acetate subsequently to extract, the ethyl acetate solution of acquisition is carried out reduced pressure concentration removing ethyl acetate solvent wherein, dry subsequently, dry body is sent in pulverizer again and pulverizes and be screened into 20 object powder, with the starch relative to its quality 4 times, mixing, granulates with 14 mesh sieves, toast 30 minutes in 60 DEG C ~ 70 DEG C baking ovens, obtain Traditional Chinese medicine immune preparation 1.
The preparation of embodiment 2 Traditional Chinese medicine immune preparation 2
By 25g, wilsonii 45g, bighead atractylodes rhizome 35g, folium panacis japonici cum caule 60g, cordate houttuynia 25g, bark of eucommia 30g, eclipta 35g, rhizome of davallia 30g, cassia twig 25g, Radix Isatidis 35g, hawthorn 35g, the membrane of a chicken's gizzard 25g, Chinese trumpet creeper 20g, Chinese yam 25g, reticulate millettia 35g, spina gleditsiae 25g, Fructus Corni 30g, peach branch 25g, frutus cnidii 35g, date 30g, tuber of stemona 20g, Gorgon fruit 25g, Galanga Galangal Seed 20g, betel nut 25g, pittosporum illicioides 35g and Horseweed Herb 30g is uniformly mixed, send in pulverizer and be crushed and screened into 40 object powder, put into marmite subsequently, add the water of 4kg, soak 3 hours, then decoct 2 hours, concoction is poured out, again add the water of 2.2kg, decoct 1 hour, filter, will the concoction obtained and the concoction obtained mixing be filtered above, reduced pressure concentration removes the water of 80% volume, add ethyl acetate subsequently to extract, the ethyl acetate solution of acquisition is carried out reduced pressure concentration removing ethyl acetate solvent wherein, dry subsequently, dry body is sent in pulverizer again and pulverizes and be screened into 20 object powder, with the starch relative to its quality 4 times, mixing, granulates with 14 mesh sieves, toast 30 minutes in 60 DEG C ~ 70 DEG C baking ovens, obtain Traditional Chinese medicine immune preparation 2.
The preparation of embodiment 3 Traditional Chinese medicine immune preparation 3
By umbellate pore furgus 35g, wilsonii 55g, bighead atractylodes rhizome 45g, folium panacis japonici cum caule 70g, cordate houttuynia 35g, bark of eucommia 40g, eclipta 45g, rhizome of davallia 40g, cassia twig 35g, Radix Isatidis 45g, hawthorn 45g, the membrane of a chicken's gizzard 35g, Chinese trumpet creeper 30g, Chinese yam 35g, reticulate millettia 45g, spina gleditsiae 35g, Fructus Corni 40g, peach branch 35g, frutus cnidii 45g, date 40g, tuber of stemona 30g, Gorgon fruit 35g, Galanga Galangal Seed 30g, betel nut 35g, pittosporum illicioides 45g and Horseweed Herb 40g is uniformly mixed, send in pulverizer and be crushed and screened into 40 object powder, put into marmite subsequently, add the water of 5kg, soak 3 hours, then decoct 2 hours, concoction is poured out, again add the water of 3kg, decoct 1 hour, filter, will the concoction obtained and the concoction obtained mixing be filtered above, reduced pressure concentration removes the water of 80% volume, add ethyl acetate subsequently to extract, the ethyl acetate solution of acquisition is carried out reduced pressure concentration removing ethyl acetate solvent wherein, dry subsequently, dry body is sent in pulverizer again and pulverizes and be screened into 20 object powder, with the starch relative to its quality 4 times, mixing, granulates with 14 mesh sieves, toast 30 minutes in 60 DEG C ~ 70 DEG C baking ovens, obtain Traditional Chinese medicine immune preparation 3.
Embodiment 4 feed 1
By rape oil 60g, dregs of beans 1000g, fish meal 60g, salt 15g, wheat flour 250g, Traditional Chinese medicine immune preparation 130g prepared by embodiment 1, bacillus subtilis 4g, the excellent profit of mineral element additive micro-(production of Beijing You Libao Bioisystech Co., Ltd) 15g, vitamine supplement FV-102 vitamin compound premix (Zhongmu Industry Co., Ltd's production) 25g, it is even that calcium dihydrogen phosphate 8g sends into stirrer for mixing, mixture homogeneity is CV%≤10%, the material mixed is modulated, temperature controls at 90 ~ 95 DEG C, modulating time 55 ~ 65 seconds, granulate subsequently, cooling, pellet composition cools, temperature controls at 20 ~ 25 DEG C, packaging, obtain feed 1.
Comparative example 1 feed 2
By rape oil 60g, dregs of beans 1000g, fish meal 60g, salt 15g, wheat flour 250g, bacillus subtilis 4g, the excellent profit of mineral element additive micro-(production of Beijing You Libao Bioisystech Co., Ltd) 15g, vitamine supplement FV-102 vitamin compound premix (Zhongmu Industry Co., Ltd's production) 25g, it is even that calcium dihydrogen phosphate 8g sends into stirrer for mixing, mixture homogeneity is CV%≤10%, the material mixed is modulated, temperature controls at 90 ~ 95 DEG C, modulating time 55 ~ 65 seconds, granulate subsequently, cooling, pellet composition cools, temperature controls at 20 ~ 25 DEG C, packaging, obtain feed 2.
Effect test
Test fish
Test uses grass carp from farm, Qingdao, fish 750 tail (average body quality 43g) of neat specification, physique stalwartness is therefrom selected after supporting temporarily, after the common salt aqueous solution sterilization of 3%, be divided at random 30 groups (often organizing 25 tails), often organize 3 repetitions, put into 30 (1.0m × 1.0m × 0.5m) net cages.If 3 control groups, remaining 7 is effect group.Net cage is placed in the cement pit of 7.5m × 7.5m × 1.5m.
Test feed
The feed 1 of test group feeding embodiment 4 preparation, the feed 2 of control group feeding comparative example 1 preparation.
Feeding and management
Duration of test, water temperature 17 ~ 25 DEG C.Every day when 9:00 and 16:00 time throw something and feed, feeding rate is 5% of weight, according to water temperature, situation of ingesting adjustment.Blowdown every day, change water 1 time, whole day is inflated, and dissolved oxygen remains on 5 more than mg/L, NH3-N<0.3 mg/L.
Sero-immunity index determining
5 tail fishes got by every case, tail vein blood, and the centrifugal 5min of 3000 r/min, gets serum for subsequent use.Superoxide dismutase (SOD), catalase (CAT), alkaline phosphatase (AKP) activity all adopts the assay method building up the kit that biotechnology research is produced by Nanjing to measure.
Data analysis and process
Result of the test represents with mean value ± standard error, and employing SPSS13.0 statistical software carries out one-way analysis of variance to test data and Dun-can ' s Multiple range test carries out statistical analysis, and the level of signifiance is 0.05.
As a result, in table 1.
Table 1 Traditional Chinese medicine immune preparation is on the impact of Grass Carp Serum SOD, CAT and AKP activity
| Group | SOD(U·mL - 1) | CAT(U·mL - 1) | AKP (King unit 100mL - 1) |
| Control group | 113.91±1.39 | 1.73±0.19 | 7.17±0.39 |
| Effect group | 135.86±1.21 | 2.62±0.21 | 9.23±0.26 |
SOD is one of antioxidase of key in Fish, it is the natural remover of superoxide radical, free radical that can be unnecessary in purged body, exempt its damage etc. to biomolecule, therefore the immune level of SOD and organism is closely related, to the immunologic function strengthening cytophagous defence capability and body, there is important function, as can be seen from Table 1, adopt Traditional Chinese medicine immune preparation provided by the invention can improve SOD level in grass carp body, CAT is the important antioxidant enzyme removing body oxygen radical, in the fish body of health, in its environment, the generation of free radical and elimination are in dynamic equilibrium.When CAT activity reduces, fish interior free yl too much can destroy some important biochemical processes in body, cause metabolic disorder, normal physiological function is lacked of proper care, in body, immune level declines, potential cause of disease is activated, fish body is fallen ill, as can be seen from Table 1, adopt Traditional Chinese medicine immune preparation provided by the invention can improve CAT level in grass carp body, AKP is a kind of important hydrolase, the hydrolysis of energy catalytic phosphatase monoester and the transfer reaction of phosphate group, to the digestion degraded of the foreign matters such as bacterium in lysosome, there is important function, and play a role in immune response, the existence of animal is had great importance, AKP is widely studied in animal immunology by as an important immune indexes all the time, as can be seen from Table 1, adopt Traditional Chinese medicine immune preparation provided by the invention can improve AKP level in grass carp body, therefore, adopt immune formulation of the present invention can significantly improve the immunologic function of grass carp.
All above-mentioned this intellectual properties of primary enforcement, not setting restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (4)
1. for improving an immune formulation for grass carp immunity function, it is characterized in that: bulk drug is made up of umbellate pore furgus, wilsonii, the bighead atractylodes rhizome, folium panacis japonici cum caule, cordate houttuynia, the bark of eucommia, eclipta, the rhizome of davallia, cassia twig, Radix Isatidis, hawthorn, the membrane of a chicken's gizzard, Chinese trumpet creeper, Chinese yam, reticulate millettia, spina gleditsiae, Fructus Corni, peach branch, frutus cnidii, date, the tuber of stemona, Gorgon fruit, Galanga Galangal Seed, betel nut, pittosporum illicioides and Horseweed Herb;
The weight of each bulk drug is respectively umbellate pore furgus 25g ~ 35g, wilsonii 45g ~ 55g, bighead atractylodes rhizome 35g ~ 45g, folium panacis japonici cum caule 60g ~ 70g, cordate houttuynia 25g ~ 35g, bark of eucommia 30g ~ 40g, eclipta 35g ~ 45g, rhizome of davallia 30g ~ 40g, cassia twig 25g ~ 35g, Radix Isatidis 35g ~ 45g, hawthorn 35g ~ 45g, the membrane of a chicken's gizzard 25g ~ 35g, Chinese trumpet creeper 20g ~ 30g, Chinese yam 25g ~ 35g, reticulate millettia 35g ~ 45g, spina gleditsiae 25g ~ 35g, Fructus Corni 30g ~ 40g, peach branch 25g ~ 35g, frutus cnidii 35g ~ 45g, date 30g ~ 40g, tuber of stemona 20g ~ 30g, Gorgon fruit 25g ~ 35g, Galanga Galangal Seed 20g ~ 30g, betel nut 25g ~ 35g, pittosporum illicioides 35g ~ 45g and Horseweed Herb 30g ~ 40g.
2. immune formulation as claimed in claim 1, is characterized in that: the weight of each bulk drug is respectively umbellate pore furgus 30g, wilsonii 50g, bighead atractylodes rhizome 40g, folium panacis japonici cum caule 65g, cordate houttuynia 30g, bark of eucommia 35g, eclipta 40g, rhizome of davallia 35g, cassia twig 30g, Radix Isatidis 40g, hawthorn 40g, the membrane of a chicken's gizzard 30g, Chinese trumpet creeper 25g, Chinese yam 30g, reticulate millettia 40g, spina gleditsiae 30g, Fructus Corni 35g, peach branch 30g, frutus cnidii 40g, date 35g, tuber of stemona 25g, Gorgon fruit 30g, Galanga Galangal Seed 25g, betel nut 30g, pittosporum illicioides 40g and Horseweed Herb 35g.
3. immune formulation as claimed in claim 1 or 2, it is characterized in that: the preparation method of described immune formulation is specially and is uniformly mixed by each bulk drug in described immune formulation, send in pulverizer and be crushed and screened into 20 order ~ 40 object powder, put into marmite subsequently, add the water of 4 ~ 6 times of quality, soak 2 ~ 4 hours, then 1 ~ 2 hour is decocted, concoction is poured out, again add the water relative to powder quality 2 ~ 4 times, decoct 30 minutes ~ 1 hour, filter, the concoction obtained and the concoction obtained above mixing will be filtered, the water of reduced pressure concentration removing 70% ~ 80% volume, add ethyl acetate subsequently to extract, the ethyl acetate solution of acquisition is carried out reduced pressure concentration removing ethyl acetate solvent wherein, dry subsequently, dry body is sent in pulverizer again and pulverizes and be screened into 20 order ~ 40 object powder, with the starch relative to its quality 3 ~ 5 times, mixing, granulate with 14 mesh sieves, toast 30 minutes in 60 DEG C ~ 70 DEG C baking ovens, obtain Traditional Chinese medicine immune preparation.
4. the preparation method of immune formulation described in claim 1 or 2, is characterized in that:
Each bulk drug in described immune formulation is uniformly mixed, send in pulverizer and be crushed and screened into 20 order ~ 40 object powder, put into marmite subsequently, add the water of 4 ~ 6 times of quality, soak 2 ~ 4 hours, then 1 ~ 2 hour is decocted, concoction is poured out, again add the water relative to powder quality 2 ~ 4 times, decoct 30 minutes ~ 1 hour, filter, the concoction obtained and the concoction obtained above mixing will be filtered, the water of reduced pressure concentration removing 70% ~ 80% volume, add ethyl acetate subsequently to extract, the ethyl acetate solution of acquisition is carried out reduced pressure concentration removing ethyl acetate solvent wherein, dry subsequently, dry body is sent in pulverizer again and pulverizes and be screened into 20 order ~ 40 object powder, with the starch relative to its quality 3 ~ 5 times, mixing, granulate with 14 mesh sieves, toast 30 minutes in 60 DEG C ~ 70 DEG C baking ovens, obtain Traditional Chinese medicine immune preparation.
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| CN101991024A (en) * | 2010-11-24 | 2011-03-30 | 广州市海维饲料有限公司 | Immune reinforced type grass carp feed |
| CN102805273A (en) * | 2012-08-31 | 2012-12-05 | 山东新希望六和集团有限公司 | Fish feed for mixed culture and preparation method of fish feed |
| CN102987094A (en) * | 2012-11-30 | 2013-03-27 | 珠海海龙生物科技有限公司 | Chinese herbal medicament immunopotentiator for high-density breeding of grass carp and preparation method thereof |
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| CN101991024A (en) * | 2010-11-24 | 2011-03-30 | 广州市海维饲料有限公司 | Immune reinforced type grass carp feed |
| CN102805273A (en) * | 2012-08-31 | 2012-12-05 | 山东新希望六和集团有限公司 | Fish feed for mixed culture and preparation method of fish feed |
| CN102987094A (en) * | 2012-11-30 | 2013-03-27 | 珠海海龙生物科技有限公司 | Chinese herbal medicament immunopotentiator for high-density breeding of grass carp and preparation method thereof |
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